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Characterization of a human ovarian carcinoma cell line: UCI 101
Citations from the Literature
ONCOLOGY Establishment and characterization of six new human endometrial adenocarcinoma cell lines Mobus V.; Gerharz C.-D.; Mitze M.; Moll R.; Pollow K.; Kother T.; Knapstein P.-G.; Kreienberg R. DEU GYNECOL ONCOL 1993 4813 (370-383) The endometrial carcinoma cell lines EC-MZ-I, 2, 3, 5, 9, and I I were established between 1986 and 1990. Four cell cultures were started from endometrial tissue, one from ascites, and one from a lymph node metastasis. Lines have to date been subcultured up to 180 times and the doubling time varies between 26 h and 3 weeks. Immunocytochemically the coexpres-
233
qualinium chloride, etoposide, 5-fluorouracil, taxol, and tumor necrosis factor was examined. Intraperitoneal transplantation of the cells into athymic mice resulted in foci of tumor on all peritoneal surfaces including the viscera and diaphragm ultimately leading to solid bulky disease with massive production of ascites. High levels of CA125 (>500 units/ml) were detected in the serum of tumor-bearing mice. Cytogenetic analysis of cultured cells shows several marker chromosomes containing deletions, duplications, and translocations. Cytologic and histologic evaluation of the xenograft revealed morphological characteristics
identical
to those of the original
tumor
Studies on ras oncogene activation in endometrial carcinoma Fujimoto 1.; Shimizu Y.; Hirai Y.; Chen J.-T.; Teshima H.;
cytokeratin (predominantly sion of simple-epithelial cytokeratin polypeptides) and vimentin intermediate filaments was detectable in all cell lines, but three lines (EC-MZ-5, 9, 1 I) expressed vimentin only at low level. By transmission electron
Hasumi K.; Masubuchi K.; Takahashi M. JPN GYNECOL ONCOL 1993 48/2 (196-202) The frequency of K-ras point mutation(PM)
microscopy the tumor cells exhibited features of epithelial differentiation. After subcutaneous transplantation into nude mice three lines (EC-MZ-I, 2, 5) produced slow-growing tumors. The histological classification of these tumors ranged
studied in 45 patients with endometrial carcinoma. In vitro amplification of target sequences of DNA extracted from endometrial cancer tissues by polymerase chain reaction and dot blotting with oligonucleotide hybridization were performed. Ten of 45 endometrial carcinomas disclosed K-ras PM at codon 12 (22.2%). Transition from GGT to GAT was most frequent in PM(41.7%). Simultaneously, double PM (GAT/GCT) were also detected in 2 cases. No relationship appeared to be present between PM and clinical prognosis such as clinical stage, histological type, histological grade of differentiation, depth of myometrial invasion, and ascitic cytology. The positive rates of lymph node metastasis tended to be higher in the group with positive PM than in the group without PM. K-ras and C-myc gene amplifications were found in 2 (5.1%) and 3 (7.7%) of 39 cases, respectively. No PM of H-ras at codons I2 and 61 was detected. Our results showed that the PM of K-ras gene at codon I2 was a fairly common event in genetic abnormality and suggested it would have some role in the progression of carcinogenesis in endometrial carcinoma.
from moderately differentiated adenocarcinoma to undifferentiated carcinoma and closely corresponded to the original tumor. Even after long-term in vitro culture, without any addition of estrogens to the culture medium, the moderately differentiated receptor-positive cell line (EC-MZ-2) retained its morphological differentiation. The cells were propagated without estrogens in the culture medium. The estrogen and progesterone receptor levels of cultured cells were determined. Three lines (EC-MZ-I, 2, 3) were positive for the progesterone receptor in low passage number only, the other cell lines were negative for both receptors. The transplantable lines were investigated for hormonal receptor expression in ovariectomized nude mice. In the moderately differentiated cell line (EC-MZ-2) we observed an enhanced expression of the estrogen receptor under optimal stimulation of the nude mouse with estradiol benzoate. There was no effect on the expression of the progesterone receptor. Characterization of a human ovarian carcinoma cell line: UC1 101 Fuchtner C.; Emma D.A.; Manetta A.; Gamboa G.; Bernstein R.; Liao S.Y. USA GYNECOL ONCOL 1993 48/2 (203-209) A new epithelial ovarian carcinoma cell line (UC1 101) has been established from the ascitic fluids and solid tumor of a patient with progressive papillary adenocarcinoma of the ovary shown previously to be refractory to combination chemotherapy consisting of cyclophosphamide, Adriamycin, and cisplatin as well as single-agent chemotherapy of taxol and high-dose cisplatin. The UC1 IO1 cell line grows well with an in vitro doubling time of 24 h. The cell line expresses the B 72.3 (Tag 72) CA125, MH99 (ESA), and E29 (EMA) cell surface antigens and AEIIAE3 cytokeratins. This cell line overexpresses (as determined by immunocytochemistry) both p-glycoprotein and the epidermal growth factor receptor. The in vitro drug response to single agents including Adriamycin, cisplatin, de-
at codon
I2 was
Combined antiproliferntive activity of Sfluorouracil and mitomyci& against primary human ovarian tumors and cell lines in a clonogenic assay Higashihara J.; Berens M.E.; Collins L.A.; Homesley H.D.; Welander C.E. USA GYNECOL ONCOL 1993 4812 (171-179) Ovarian tumors from 389 patients were successfully grown in a human tumor clonogenic assay (HTCA). Specimens from patients with or without prior chemotherapy showed similar chemosensitivity patterns. Excluding drugs commonly used for first-line chemotherapy, 5-fluorouracil (5FU) and mitomycinC (MMC) are among the active second-line agents, based upon HCTA data. Using this in vitro information from primary tumor specimens, two human ovarian cancer cell lines were used to study in detail the interactions of combined 5-FU and MMC. Drug scheduling which maximized combined antitumor activity was studied. Combined 5-FU and MMC revealed positive drug interactions most consistently when both drugs were used simultaneously with long-term exposure. Pulse treatment with MMC (I h) followed by continuous 5-FU exposure W J Gynecol Obstet 43
ONCOLOGY Establishment and characterization of six new human endometrial adenocarcinoma cell lines Mobus V.; Gerharz C.-D.; Mitze M.; Moll R.; Pollow K.; Kother T.; Knapstein P.-G.; Kreienberg R. DEU GYNECOL ONCOL 1993 4813 (370-383) The endometrial carcinoma cell lines EC-MZ-I, 2, 3, 5, 9, and I I were established between 1986 and 1990. Four cell cultures were started from endometrial tissue, one from ascites, and one from a lymph node metastasis. Lines have to date been subcultured up to 180 times and the doubling time varies between 26 h and 3 weeks. Immunocytochemically the coexpres-
233
qualinium chloride, etoposide, 5-fluorouracil, taxol, and tumor necrosis factor was examined. Intraperitoneal transplantation of the cells into athymic mice resulted in foci of tumor on all peritoneal surfaces including the viscera and diaphragm ultimately leading to solid bulky disease with massive production of ascites. High levels of CA125 (>500 units/ml) were detected in the serum of tumor-bearing mice. Cytogenetic analysis of cultured cells shows several marker chromosomes containing deletions, duplications, and translocations. Cytologic and histologic evaluation of the xenograft revealed morphological characteristics
identical
to those of the original
tumor
Studies on ras oncogene activation in endometrial carcinoma Fujimoto 1.; Shimizu Y.; Hirai Y.; Chen J.-T.; Teshima H.;
cytokeratin (predominantly sion of simple-epithelial cytokeratin polypeptides) and vimentin intermediate filaments was detectable in all cell lines, but three lines (EC-MZ-5, 9, 1 I) expressed vimentin only at low level. By transmission electron
Hasumi K.; Masubuchi K.; Takahashi M. JPN GYNECOL ONCOL 1993 48/2 (196-202) The frequency of K-ras point mutation(PM)
microscopy the tumor cells exhibited features of epithelial differentiation. After subcutaneous transplantation into nude mice three lines (EC-MZ-I, 2, 5) produced slow-growing tumors. The histological classification of these tumors ranged
studied in 45 patients with endometrial carcinoma. In vitro amplification of target sequences of DNA extracted from endometrial cancer tissues by polymerase chain reaction and dot blotting with oligonucleotide hybridization were performed. Ten of 45 endometrial carcinomas disclosed K-ras PM at codon 12 (22.2%). Transition from GGT to GAT was most frequent in PM(41.7%). Simultaneously, double PM (GAT/GCT) were also detected in 2 cases. No relationship appeared to be present between PM and clinical prognosis such as clinical stage, histological type, histological grade of differentiation, depth of myometrial invasion, and ascitic cytology. The positive rates of lymph node metastasis tended to be higher in the group with positive PM than in the group without PM. K-ras and C-myc gene amplifications were found in 2 (5.1%) and 3 (7.7%) of 39 cases, respectively. No PM of H-ras at codons I2 and 61 was detected. Our results showed that the PM of K-ras gene at codon I2 was a fairly common event in genetic abnormality and suggested it would have some role in the progression of carcinogenesis in endometrial carcinoma.
from moderately differentiated adenocarcinoma to undifferentiated carcinoma and closely corresponded to the original tumor. Even after long-term in vitro culture, without any addition of estrogens to the culture medium, the moderately differentiated receptor-positive cell line (EC-MZ-2) retained its morphological differentiation. The cells were propagated without estrogens in the culture medium. The estrogen and progesterone receptor levels of cultured cells were determined. Three lines (EC-MZ-I, 2, 3) were positive for the progesterone receptor in low passage number only, the other cell lines were negative for both receptors. The transplantable lines were investigated for hormonal receptor expression in ovariectomized nude mice. In the moderately differentiated cell line (EC-MZ-2) we observed an enhanced expression of the estrogen receptor under optimal stimulation of the nude mouse with estradiol benzoate. There was no effect on the expression of the progesterone receptor. Characterization of a human ovarian carcinoma cell line: UC1 101 Fuchtner C.; Emma D.A.; Manetta A.; Gamboa G.; Bernstein R.; Liao S.Y. USA GYNECOL ONCOL 1993 48/2 (203-209) A new epithelial ovarian carcinoma cell line (UC1 101) has been established from the ascitic fluids and solid tumor of a patient with progressive papillary adenocarcinoma of the ovary shown previously to be refractory to combination chemotherapy consisting of cyclophosphamide, Adriamycin, and cisplatin as well as single-agent chemotherapy of taxol and high-dose cisplatin. The UC1 IO1 cell line grows well with an in vitro doubling time of 24 h. The cell line expresses the B 72.3 (Tag 72) CA125, MH99 (ESA), and E29 (EMA) cell surface antigens and AEIIAE3 cytokeratins. This cell line overexpresses (as determined by immunocytochemistry) both p-glycoprotein and the epidermal growth factor receptor. The in vitro drug response to single agents including Adriamycin, cisplatin, de-
at codon
I2 was
Combined antiproliferntive activity of Sfluorouracil and mitomyci& against primary human ovarian tumors and cell lines in a clonogenic assay Higashihara J.; Berens M.E.; Collins L.A.; Homesley H.D.; Welander C.E. USA GYNECOL ONCOL 1993 4812 (171-179) Ovarian tumors from 389 patients were successfully grown in a human tumor clonogenic assay (HTCA). Specimens from patients with or without prior chemotherapy showed similar chemosensitivity patterns. Excluding drugs commonly used for first-line chemotherapy, 5-fluorouracil (5FU) and mitomycinC (MMC) are among the active second-line agents, based upon HCTA data. Using this in vitro information from primary tumor specimens, two human ovarian cancer cell lines were used to study in detail the interactions of combined 5-FU and MMC. Drug scheduling which maximized combined antitumor activity was studied. Combined 5-FU and MMC revealed positive drug interactions most consistently when both drugs were used simultaneously with long-term exposure. Pulse treatment with MMC (I h) followed by continuous 5-FU exposure W J Gynecol Obstet 43