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Characterization of the Heterogeneity of R3327 Rat Prostatic Tumors Derived From Single-Cell Clones
0022-534 7 /86/1352-0441$02.00/0 \/ol. 135, February
THE JOURNAL OF UROLOGY
Copyright© 1986 by The Wiiliams & Wilkins Co.
Printed in U.S.A.
ABSTRACTS ONCOLOGY AND CHEMOTHERAPY Thiotepa Versus Adriamycin Versus Cis-Platinum in the Intravesical Prophylaxis of Superficial Bladder Tumors B. LLOPIS, J. GALLEGO, J. A. MOMPO, F. BORONAT AND J. F. JIMENEZ, Department of Urology, La Fe Hospital, Valencia, Spain Eur. Urol., 11: 73-78 (Mar.-Apr.) 1985 Life expectancy for patients with superficial bladder tumors is high, at an estimated 60 to 70 per cent after 5 years. The main problem with these tumors is their high tendency to relapse, with 70 per cent of the patients having recurrence if treated only with transurethral resection and with progression of invasive carcinoma in 10 per cent. The authors used intravesical chemotherapy for 184 patients with superficial bladder cancer (stages pTa and pTl): 92 with primary and 92 with recurrent tumors. After transurethral resection of all visible lesions, endovesical chemotherapy with thiotepa, doxorubicin or cis-platinum was started 15 and 30 days postoperatively. There were no statistically significant differences among any of the 3 chemotherapies at the dose intervals used (50 mg. doxorubicin and cis-platinum weekly for 4 weeks and 50 mg. monthly for 11 months, and 50 mg. thiotepa weekly for 8 weeks). Cystoscopic revision was done every 3 months. When disease recurred a transurethral resection was performed again and the patients continued with the same protocol. Only cisplatinum proved to be slightly more effective in patients with primary high grade tumors. W. J. C. 8 tables, 41 references
Malignant Mesothelioma of Testicular Tunica I.
VAKALIKOS, E. DESTOUNI, K. VALASSIS, C. EFTICHIADOU, A. CHARALAMBOPOULOS AND N. SALEM, Departments of Urology and Radiology, Theagenio Cancer Institute, Thessaloniki, Greece
instability as a mechanism of heterogeneity using tissue culture lines derived from single cells of the Dunning R3327 prostatic cell culture. Four cell lines were developed and injected into castrate or intact rats. Morphological and cytometric means were used to study resultant tumors. Only slight differences were seen among cell cultures by transmission or electron microscopy. Cultured cells uniformly were diploid by staining and flow cytometric techniques. On the other hand, tumors showed morphological and genetic heterogeneity. The authors conclude that changes in genotype and phenotype occurred in tumors derived from single cells. These changes may have occurred while cells still were in culture. J. H. N. 22 figures, 17 references
The Relationship of Natural Killer Cells to Metastasis of a Transplantable Prostate Adenocarcinoma A. C. WADE, M. A. SCHMIDT AND M. POLLARD, Lobund Laboratories, University of Notre Dame, Notre Dame, Indiana Prostate, 7: 53-61, 1985 Natural killer cells have been proposed to have a significant role in the inhibition of metastasis. The prostatic adenocarcinoma (P A-11) in the Lobund-Wistar rat provides a unique model of spontaneous metastasis in which to study natural killer response. Cultured PA-11 tumor cells were shown to be resistant to natural killer lysis in vitro, and enhancement or inhibition of natural killer reactivity in vivo using drugs or antiserum did not change the rate or extent of metastasis evident at autopsy. Exposure to PA-11 tumor cells, supernatants from cultured tumor cells or sera from rats with advanced P A-11 in vitro did not result in inhibition of natural killer activity. Exposure to PA-11 tumor cells in vivo also did not cause suppression of natural killer activity. These data indicate that in the PA-11/Lobund-Wistar rat system metastasis is independent of natural killer activity. W. W. K. 1 figure, 3 tables, 17 references
J. Surg. Oncol., 29: 264 (Aug.) 1985
A 26-year-old man with malignant mesothelioma of the testicular tunica is presented. An operation was followed by local radiotherapy. The patient remained free of disease 1 year after the initial diagnosis. W. W. K. 2 figures, 3 references
Characterization of the Heterogeneity of R3327 Rat Prostatic Tumors Derived From Single-Cell Clones S. A.
THOMPSON, M. P. JOHNSON, P. M. HEIDGER, JR. AND D. M. LUBAROFF, Departments of Anatomy, Urology
and Microbiology, College of Medicine, University of Iowa, Iowa City, Iowa Prostate, 6: 369-387, 1985 The Dunning R3327 rat prostatic carcinoma provides a frequently used model for the study of human prostatic cancer. Cellular adaptation (host factors) and/or genetic changes in tumor cells may be responsible for diversity (heterogeneity) in cellular morphology and behavior. The authors studied genetic 441
Pro§tatic Carcinoma Metastatic to Bone: Sensitivity and Specificity of Prostate-Specific Antigen and Prostatic Acid Phosphatase in Decafoified Material
N. T. SHAH, S. E. TUTTLE, S. L. STROBEL AND L. GANDHI, Department of Pathology, The Ohio State University Hospitals, Columbus, Ohio J. Surg. Oncol., 29: 265-268 (Aug.) 1985 Decalcified bone marrow biopsies containing metastatic tumor from 36 patients were stained for prostate-specific antigen and prostatic acid phosphatase with the avidin biotin complex immunoperoxidase technique. Of these patients 22 had known prostatic and 10 had known nonprostatic primaries, and 4 female patients had unknown primaries. Prostate-specific antigen was identified in 19 of 22 metastatic prostatic carcinomas (86 per cent), while prostatic acid phosphatase was present in only 8 (36 per cent). None of the 14 patients with nonprostatic or unknown primaries had positive staining for either antigen. lmmunoperoxidase staining for prostate-specific antigen was sensitive and specific in the diagnosis of metastatic prostatic
THE JOURNAL OF UROLOGY
Copyright© 1986 by The Wiiliams & Wilkins Co.
Printed in U.S.A.
ABSTRACTS ONCOLOGY AND CHEMOTHERAPY Thiotepa Versus Adriamycin Versus Cis-Platinum in the Intravesical Prophylaxis of Superficial Bladder Tumors B. LLOPIS, J. GALLEGO, J. A. MOMPO, F. BORONAT AND J. F. JIMENEZ, Department of Urology, La Fe Hospital, Valencia, Spain Eur. Urol., 11: 73-78 (Mar.-Apr.) 1985 Life expectancy for patients with superficial bladder tumors is high, at an estimated 60 to 70 per cent after 5 years. The main problem with these tumors is their high tendency to relapse, with 70 per cent of the patients having recurrence if treated only with transurethral resection and with progression of invasive carcinoma in 10 per cent. The authors used intravesical chemotherapy for 184 patients with superficial bladder cancer (stages pTa and pTl): 92 with primary and 92 with recurrent tumors. After transurethral resection of all visible lesions, endovesical chemotherapy with thiotepa, doxorubicin or cis-platinum was started 15 and 30 days postoperatively. There were no statistically significant differences among any of the 3 chemotherapies at the dose intervals used (50 mg. doxorubicin and cis-platinum weekly for 4 weeks and 50 mg. monthly for 11 months, and 50 mg. thiotepa weekly for 8 weeks). Cystoscopic revision was done every 3 months. When disease recurred a transurethral resection was performed again and the patients continued with the same protocol. Only cisplatinum proved to be slightly more effective in patients with primary high grade tumors. W. J. C. 8 tables, 41 references
Malignant Mesothelioma of Testicular Tunica I.
VAKALIKOS, E. DESTOUNI, K. VALASSIS, C. EFTICHIADOU, A. CHARALAMBOPOULOS AND N. SALEM, Departments of Urology and Radiology, Theagenio Cancer Institute, Thessaloniki, Greece
instability as a mechanism of heterogeneity using tissue culture lines derived from single cells of the Dunning R3327 prostatic cell culture. Four cell lines were developed and injected into castrate or intact rats. Morphological and cytometric means were used to study resultant tumors. Only slight differences were seen among cell cultures by transmission or electron microscopy. Cultured cells uniformly were diploid by staining and flow cytometric techniques. On the other hand, tumors showed morphological and genetic heterogeneity. The authors conclude that changes in genotype and phenotype occurred in tumors derived from single cells. These changes may have occurred while cells still were in culture. J. H. N. 22 figures, 17 references
The Relationship of Natural Killer Cells to Metastasis of a Transplantable Prostate Adenocarcinoma A. C. WADE, M. A. SCHMIDT AND M. POLLARD, Lobund Laboratories, University of Notre Dame, Notre Dame, Indiana Prostate, 7: 53-61, 1985 Natural killer cells have been proposed to have a significant role in the inhibition of metastasis. The prostatic adenocarcinoma (P A-11) in the Lobund-Wistar rat provides a unique model of spontaneous metastasis in which to study natural killer response. Cultured PA-11 tumor cells were shown to be resistant to natural killer lysis in vitro, and enhancement or inhibition of natural killer reactivity in vivo using drugs or antiserum did not change the rate or extent of metastasis evident at autopsy. Exposure to PA-11 tumor cells, supernatants from cultured tumor cells or sera from rats with advanced P A-11 in vitro did not result in inhibition of natural killer activity. Exposure to PA-11 tumor cells in vivo also did not cause suppression of natural killer activity. These data indicate that in the PA-11/Lobund-Wistar rat system metastasis is independent of natural killer activity. W. W. K. 1 figure, 3 tables, 17 references
J. Surg. Oncol., 29: 264 (Aug.) 1985
A 26-year-old man with malignant mesothelioma of the testicular tunica is presented. An operation was followed by local radiotherapy. The patient remained free of disease 1 year after the initial diagnosis. W. W. K. 2 figures, 3 references
Characterization of the Heterogeneity of R3327 Rat Prostatic Tumors Derived From Single-Cell Clones S. A.
THOMPSON, M. P. JOHNSON, P. M. HEIDGER, JR. AND D. M. LUBAROFF, Departments of Anatomy, Urology
and Microbiology, College of Medicine, University of Iowa, Iowa City, Iowa Prostate, 6: 369-387, 1985 The Dunning R3327 rat prostatic carcinoma provides a frequently used model for the study of human prostatic cancer. Cellular adaptation (host factors) and/or genetic changes in tumor cells may be responsible for diversity (heterogeneity) in cellular morphology and behavior. The authors studied genetic 441
Pro§tatic Carcinoma Metastatic to Bone: Sensitivity and Specificity of Prostate-Specific Antigen and Prostatic Acid Phosphatase in Decafoified Material
N. T. SHAH, S. E. TUTTLE, S. L. STROBEL AND L. GANDHI, Department of Pathology, The Ohio State University Hospitals, Columbus, Ohio J. Surg. Oncol., 29: 265-268 (Aug.) 1985 Decalcified bone marrow biopsies containing metastatic tumor from 36 patients were stained for prostate-specific antigen and prostatic acid phosphatase with the avidin biotin complex immunoperoxidase technique. Of these patients 22 had known prostatic and 10 had known nonprostatic primaries, and 4 female patients had unknown primaries. Prostate-specific antigen was identified in 19 of 22 metastatic prostatic carcinomas (86 per cent), while prostatic acid phosphatase was present in only 8 (36 per cent). None of the 14 patients with nonprostatic or unknown primaries had positive staining for either antigen. lmmunoperoxidase staining for prostate-specific antigen was sensitive and specific in the diagnosis of metastatic prostatic