Chemical methods in bacterial systematics

Chemical methods in bacterial systematics

BOOK 611 REVIEWS scientist. There is also a delightful lecture, previously unpublished, pointing out that there are still some advocates of the ben...

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BOOK

611

REVIEWS

scientist. There is also a delightful lecture, previously unpublished, pointing out that there are still some advocates of the beneficial effects of ionising radiation and what flimsy evidence their theories are based on, quoting as an example the case of the thermal baths of Bad Gastein. The volume is brought to a close by a 2%page biography, written by his son, and by a complete list of Engelbert Broda’s publications. This reviewer was a personal friend of Professor Broda and the perusal of this volume brought again home the loss his death was for his friends, the scientific community and especially for his country, Austria. MICHAEL

Journal of Chromatography, 351 (1986) 611 Elsevier Science Publishers B.V.. Amsterdam CHROM.

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Printed

LEDERER

in The Netherlands

18 307

Book Review

Chemical methods in bacterial systematics, edited by M. Goodfellow

and D. E. Minnikin; Academic Press, London, New York, Tokyo, 1985, 412 pp., price U.S. $69.50, E45.00, ISBN 0-12-289675-O.

The basis of this volume is a poster session on the topic “The Use of Chemotaxonomic Methods for Bacteria”, held during the Summer Conference of the Society of Applied Bacteriology at Heriot-Watt University, Edinburgh, in July 1983. It makes interesting reading, not only for the bacteriologist, but also for the chromatographer. The nineteetrchapters (by various authors) show in numerous examples how chromatographic methods can be used in chemosystematics. The reviewer is impressed that most of the methods used are flat-bed methods, both thin-layer chromatographic and electrophoretic methods, and usually one-dimensional, e.g., for whole-cell protein patterns of streptococcus or for patterns of complex mycobacterial lipids (here also superb two-dimensional patterns are shown). Gas chromatography is employed for examining sugar patterns, fatty acids and headspace techniques for volatile end products. High-performance liquid chromatography seems to have found relatively little use (so far), but some chromatograms are shown, e.g., for the ubiquinones from Legionella pneumophila and hydrogenated menaquinones from M. echinospora. One chapter is devoted to pyrolysismass spectrometry. This seems still to be in an early stage in bacteriology but has promise as it provides results very rapidly.