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Chitosan scaffold improved by blending with peg and CMC
S592
Abstracts / Journal of Biotechnology 136S (2008) S589–S601
References
VI6-P-009
Cozzolino, R., Malvagn, P., 2006. Structural analysis of the polysaccharides from Echinacea angustifolia radix. Carbohyd. Polym. 65, 263–272. Ishurd, O., Kermagi, A., Elghazoun, M., Kennedy, J.F., 2006. Structural of a glucomannan from Lupinus varius seed. Carbohyd. Polym. 65, 410–413. Wang, Z.J., Luo, D.H., Liang, Z.Y., 2004. Structure of polysaccharides from the fruiting body of Hericium erinaceus. Carbohyd. Polym. 57, 241–247.
Chitosan scaffold improved by blending with peg and CMC
doi:10.1016/j.jbiotec.2008.07.1193 VI6-P-008 Biosynthesis and characterization of polyhydroxyalkanoates in the marine bacterium Pseudoaltermonas sp. SM9913 Qian Wang, Hanxing Zhang, Quan Chen, Xiulan Chen, Yuzhong Zhang, Qingsheng Qi ∗ State Key Laboratory of Microbial Technology, Life Science School, Shandong University, Jinan 250100, PR China Poly(hydroxyalkanoate)s (PHAs) are biodegradable and biocompatible polyesters produced by many bacteria as storage compounds for carbon and energy under unbalanced growth conditions. They have attracted much attention as environmentally friendly “green” thermoplastic to be used in a wide range of agriculture, marine and medical application. Recently, PHAs have been identified in several kinds of marine microorganisms. The marine bacterium Pseudoaltermonas sp. SM9913 was investigated for the synthesis of polyhydroxyalkanoates (PHAs), using glucose, decanoate or olive oil as the sole source of carbon, respectively, in a sea water based LB medium culture. The biopolymer accumulated in Pseudoaltermonas sp. SM9913 cells was detected by Nile red staining and transmission electron microscope (TEM) analysis. From GC–MS analysis, it was found that this strain produced a copolymer of 3-hydroxydecanoate(HD) and 3hydroxydodecanoate(HDD) when any of the three carbon sources was supplied. The cellular polymer concentration was 3.10%, 1.89%, 2.57% of the CDW when glucose, decanoate and olive oil were provided as carbon sources, respectively. 3-HD unit and 3-HDD unit contribute to approximately 15% and 85% of the mole fractions in the polymer. This work is the first report determining the capacity of Pseudoaltermonas sp. SM9913 to synthesize PHAs. References Alvarez, H.M., Pucci, O.H., Steinbüchel, A., 1997. Lipid storage compounds in marine bacteria. Appl. Microbiol. Biotechnol. 47, 132–139. Chen, X.L., Zhang, Y.Z., Wang, Y.T., Gao, P.J., Luan, X.W., 2001. Psychrotrophilic alkaline protease from a deep sea psychrotrophilic strain Pseudomonas sp. SM9913. Mar. Sci. 25 (1), 4–8. Madison, L., Huisman, G.W., 1999. Metabolic engineering of poly(3-hydroxyalkanoates):from DNA to plastic. Microbiol. Mol. Biol. Rev. 63, 21–53.
doi:10.1016/j.jbiotec.2008.07.1194
Hua Hong, Changsheng Liu ∗ , Wenjing Wu Key Laboratory for Ultrafine Materials of Ministry of Education, Engineering Research Center for Biomedical Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, China E-mail address: [email protected] (C. Liu). Inferior compliant ability and low biodegradability limited chitosan usage, especially as GTR scaffold. The objective of this work was to firstly enhance the flexibility of chitosan scaffold via mixing chitosan with polyethylene glycol (PEG) and secondly accelerates the degradation rate by blending binary chitosan-PEG mixture with carboxymethyl chitosan (CMC) (Ponticiello et al., 2000). The degradation studies were performed at 37 ◦ C at 240 rpm with permanent stirring to mimic the physiologic conditions in physiological media. During different period of degradation, the morphology, tensile strength and biocompatibility for the samples were measured, respectively (Yan et al., 2005). Degradation for 5 weeks, the blend scaffold shape maintained integrity which enabled the scaffold functioned as a physical supporter. The SEM showed that CPM blend scaffold displayed more defects and holes in comparison with the chitosan scaffold after degrading for 3 weeks in degradation medium. At the end of degradation about 5 weeks, the tensile strength for the CPM blend scaffold was about 12 MPa. The max tensile strength for human periodontal ligament was 5 × 104 Pa appeared at the tooth cervix (Xu et al., 2004). So the strength of blend scaffold made on this paper could meet the need of practicality. The mechanical tests also showed that CPM scaffold more compliant than chitosan one. The compliance in extension was 1.6 × 10−2 for chitosan scaffold and 3.1 × 10−2 for CPM scaffold. The cell (C2C12) proliferation results showed that the CPM scaffold presented good biocompatibility and cell growth very well. The adding of PEG improved the flexibility and appearance of chitosan scaffold. The CMC enhanced weight loss and accelerated the degradation rate of chitosan scaffold, it was an effectively way to control the degradation rate and to improve flexibility of chitosan scaffold by changing the amount of PEG and CMC. CPM scaffold showed significant improvement in degradation and flexibility comparable to chitosan membrane. References Ponticiello, M.S., Schinagl, R.M., Kadiyala, S., Barry, F.P., 2000. Gelatin-based resorbable sponge as a carrier matrix for human mesenchymal stem cells in cartilage regeneration therapy. J. Biomed. Mater. Res. 52, 246–255. Xu, Y., Hong, H., Qian, Y., Liu, C.S., 2004. Preparation and characterisation of biomedical chitosan film. J. Funct. Polym. 3, 55–60. Yan, H., Stella, O., Sundararajan, V., 2005. In vitro characterization of chitosan–gelatin scaffolds for tissue engineering. Biomaterials 26, 7616–7627.
doi:10.1016/j.jbiotec.2008.07.1195
Abstracts / Journal of Biotechnology 136S (2008) S589–S601
References
VI6-P-009
Cozzolino, R., Malvagn, P., 2006. Structural analysis of the polysaccharides from Echinacea angustifolia radix. Carbohyd. Polym. 65, 263–272. Ishurd, O., Kermagi, A., Elghazoun, M., Kennedy, J.F., 2006. Structural of a glucomannan from Lupinus varius seed. Carbohyd. Polym. 65, 410–413. Wang, Z.J., Luo, D.H., Liang, Z.Y., 2004. Structure of polysaccharides from the fruiting body of Hericium erinaceus. Carbohyd. Polym. 57, 241–247.
Chitosan scaffold improved by blending with peg and CMC
doi:10.1016/j.jbiotec.2008.07.1193 VI6-P-008 Biosynthesis and characterization of polyhydroxyalkanoates in the marine bacterium Pseudoaltermonas sp. SM9913 Qian Wang, Hanxing Zhang, Quan Chen, Xiulan Chen, Yuzhong Zhang, Qingsheng Qi ∗ State Key Laboratory of Microbial Technology, Life Science School, Shandong University, Jinan 250100, PR China Poly(hydroxyalkanoate)s (PHAs) are biodegradable and biocompatible polyesters produced by many bacteria as storage compounds for carbon and energy under unbalanced growth conditions. They have attracted much attention as environmentally friendly “green” thermoplastic to be used in a wide range of agriculture, marine and medical application. Recently, PHAs have been identified in several kinds of marine microorganisms. The marine bacterium Pseudoaltermonas sp. SM9913 was investigated for the synthesis of polyhydroxyalkanoates (PHAs), using glucose, decanoate or olive oil as the sole source of carbon, respectively, in a sea water based LB medium culture. The biopolymer accumulated in Pseudoaltermonas sp. SM9913 cells was detected by Nile red staining and transmission electron microscope (TEM) analysis. From GC–MS analysis, it was found that this strain produced a copolymer of 3-hydroxydecanoate(HD) and 3hydroxydodecanoate(HDD) when any of the three carbon sources was supplied. The cellular polymer concentration was 3.10%, 1.89%, 2.57% of the CDW when glucose, decanoate and olive oil were provided as carbon sources, respectively. 3-HD unit and 3-HDD unit contribute to approximately 15% and 85% of the mole fractions in the polymer. This work is the first report determining the capacity of Pseudoaltermonas sp. SM9913 to synthesize PHAs. References Alvarez, H.M., Pucci, O.H., Steinbüchel, A., 1997. Lipid storage compounds in marine bacteria. Appl. Microbiol. Biotechnol. 47, 132–139. Chen, X.L., Zhang, Y.Z., Wang, Y.T., Gao, P.J., Luan, X.W., 2001. Psychrotrophilic alkaline protease from a deep sea psychrotrophilic strain Pseudomonas sp. SM9913. Mar. Sci. 25 (1), 4–8. Madison, L., Huisman, G.W., 1999. Metabolic engineering of poly(3-hydroxyalkanoates):from DNA to plastic. Microbiol. Mol. Biol. Rev. 63, 21–53.
doi:10.1016/j.jbiotec.2008.07.1194
Hua Hong, Changsheng Liu ∗ , Wenjing Wu Key Laboratory for Ultrafine Materials of Ministry of Education, Engineering Research Center for Biomedical Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, China E-mail address: [email protected] (C. Liu). Inferior compliant ability and low biodegradability limited chitosan usage, especially as GTR scaffold. The objective of this work was to firstly enhance the flexibility of chitosan scaffold via mixing chitosan with polyethylene glycol (PEG) and secondly accelerates the degradation rate by blending binary chitosan-PEG mixture with carboxymethyl chitosan (CMC) (Ponticiello et al., 2000). The degradation studies were performed at 37 ◦ C at 240 rpm with permanent stirring to mimic the physiologic conditions in physiological media. During different period of degradation, the morphology, tensile strength and biocompatibility for the samples were measured, respectively (Yan et al., 2005). Degradation for 5 weeks, the blend scaffold shape maintained integrity which enabled the scaffold functioned as a physical supporter. The SEM showed that CPM blend scaffold displayed more defects and holes in comparison with the chitosan scaffold after degrading for 3 weeks in degradation medium. At the end of degradation about 5 weeks, the tensile strength for the CPM blend scaffold was about 12 MPa. The max tensile strength for human periodontal ligament was 5 × 104 Pa appeared at the tooth cervix (Xu et al., 2004). So the strength of blend scaffold made on this paper could meet the need of practicality. The mechanical tests also showed that CPM scaffold more compliant than chitosan one. The compliance in extension was 1.6 × 10−2 for chitosan scaffold and 3.1 × 10−2 for CPM scaffold. The cell (C2C12) proliferation results showed that the CPM scaffold presented good biocompatibility and cell growth very well. The adding of PEG improved the flexibility and appearance of chitosan scaffold. The CMC enhanced weight loss and accelerated the degradation rate of chitosan scaffold, it was an effectively way to control the degradation rate and to improve flexibility of chitosan scaffold by changing the amount of PEG and CMC. CPM scaffold showed significant improvement in degradation and flexibility comparable to chitosan membrane. References Ponticiello, M.S., Schinagl, R.M., Kadiyala, S., Barry, F.P., 2000. Gelatin-based resorbable sponge as a carrier matrix for human mesenchymal stem cells in cartilage regeneration therapy. J. Biomed. Mater. Res. 52, 246–255. Xu, Y., Hong, H., Qian, Y., Liu, C.S., 2004. Preparation and characterisation of biomedical chitosan film. J. Funct. Polym. 3, 55–60. Yan, H., Stella, O., Sundararajan, V., 2005. In vitro characterization of chitosan–gelatin scaffolds for tissue engineering. Biomaterials 26, 7616–7627.
doi:10.1016/j.jbiotec.2008.07.1195