- Email: [email protected]
Cholecystokinin (CCK) induces short-lasting excitation in repetitive firing neurons of the rat nucleus tractus solitarius (NTS)
Sl6
615
CENTRAL
ANTIDIURETIC ACTION VIA TACHYKININ NK-3 RECEPTOR IN RAT TA PARAVENTRICULAR NUCLEUS. SAITO’ , YASlJO TAKEDA”. YUKIO TAKANO’ AND HIRO-0 KAMIYA’. ‘Dept. of Pharmacol.. Faculty of Pharmaceutical
Sci.. Fukuoka Univ.. Fukuoka 814-80, 2Dept. of Physiol.. Sch. of Med.. Keio Univ.. Tokyo 160
!J.aM!L we have previously reported that intracerebroventricular (i.c.v.) injections of senktide, a tachykinin NK-3 agonist, showed an antidiuretic action in water-loaded rats (4.5 % body weight)(l). In the present study, we examined the site of action of senktide in the brain on antidiuretic actions in conscious rats. The microinjection of a tachykinin NK-3 agonist, senktide (0.2 - 0.002 nmol) into the paraventricular nucleus (PVN) inhibited urine output in a dose-dependent manner. But substance P (0.2 nmol), tachykinin NK-1 agonist did not inhibit urine output. The antidiuretic effect of senktide was inhibited by pretreatment with a non-peptide vasopressin V2 antagonist (i.v.), OPC-31260. These results suggest that the site of action of the NK-3 agonists for its antidiuretic effect may be PVN of the hypothalamus and that its action may be due to stimulation of (I) Neurosci.Lett.159: 187-190 (1993). vasopressin secretion from the pituitary gland.
616
SIMULTANEOUS MEASUREMENT OF ACETYLCHOLINE AND CHOLECYSTOKININ IN RAT FRONTAL CORTEX WITH AN IN VIVO MICRODIALYSIS. JIAN KAI J,IU AND .
-
IJmYokohama Yokohama 736. JaDan
City
Universitv.
.
32-2
Set0
wawa-ku.
Acetylcholine (ACh) and cholecystokinin (CCK) in mammalian frontal cortex have important physiological roles on memory and learning. Systemic treatment with muscarinic antagonist scopolamine and cerebral activator indeloxazine increased ACh output in rat frontal cortex studied with an in vivo microdialysis method. To detect CCK immunoreactivity (CCKLI) in the dialysate of rat frontal cortex, we a highly sensitive enzyme immunoassay using C terminal-directed developed gastrin/CCK antiserum. This assay method was detectable as little as 0.2 Pg CCKa/tube. These results that this method is applicable suggest assay simultaneously to determine both ACh and CCKLI output in the dialysate.
617
CHOLECYSTOKININ(CCK)INDUCESSHORT-LASTING EXCITATION INREPETITIVEFIRING NEURONS OF THE RAT NUCLEUS TRACTUS SOLITARIUS (NTS). KENJI SUZUKI’. SHUJI AOU’, NOBUAKI SHIMIZU2eAND, 'Deut. ofPhvsiol., Kvushu Univ.. Facultv of Medicine. Fukuoka 8 12, Jaoan. 2Deot. of Chem. & Chem. Ene.. Kanazawa Univ.. Kanazawa 920. Jauan.
The medial NTS (mNTS) neurons predominantly receive gastrointestinal inputs and possess CCKA receptors which mediate asatietysignal. We performed extracellularand intracellular recordings fromneurons of the mNTS in vitroto characterize their electrophysiological properties and CCK sensitivity. CCK (
615
CENTRAL
ANTIDIURETIC ACTION VIA TACHYKININ NK-3 RECEPTOR IN RAT TA PARAVENTRICULAR NUCLEUS. SAITO’ , YASlJO TAKEDA”. YUKIO TAKANO’ AND HIRO-0 KAMIYA’. ‘Dept. of Pharmacol.. Faculty of Pharmaceutical
Sci.. Fukuoka Univ.. Fukuoka 814-80, 2Dept. of Physiol.. Sch. of Med.. Keio Univ.. Tokyo 160
!J.aM!L we have previously reported that intracerebroventricular (i.c.v.) injections of senktide, a tachykinin NK-3 agonist, showed an antidiuretic action in water-loaded rats (4.5 % body weight)(l). In the present study, we examined the site of action of senktide in the brain on antidiuretic actions in conscious rats. The microinjection of a tachykinin NK-3 agonist, senktide (0.2 - 0.002 nmol) into the paraventricular nucleus (PVN) inhibited urine output in a dose-dependent manner. But substance P (0.2 nmol), tachykinin NK-1 agonist did not inhibit urine output. The antidiuretic effect of senktide was inhibited by pretreatment with a non-peptide vasopressin V2 antagonist (i.v.), OPC-31260. These results suggest that the site of action of the NK-3 agonists for its antidiuretic effect may be PVN of the hypothalamus and that its action may be due to stimulation of (I) Neurosci.Lett.159: 187-190 (1993). vasopressin secretion from the pituitary gland.
616
SIMULTANEOUS MEASUREMENT OF ACETYLCHOLINE AND CHOLECYSTOKININ IN RAT FRONTAL CORTEX WITH AN IN VIVO MICRODIALYSIS. JIAN KAI J,IU AND .
-
IJmYokohama Yokohama 736. JaDan
City
Universitv.
.
32-2
Set0
wawa-ku.
Acetylcholine (ACh) and cholecystokinin (CCK) in mammalian frontal cortex have important physiological roles on memory and learning. Systemic treatment with muscarinic antagonist scopolamine and cerebral activator indeloxazine increased ACh output in rat frontal cortex studied with an in vivo microdialysis method. To detect CCK immunoreactivity (CCKLI) in the dialysate of rat frontal cortex, we a highly sensitive enzyme immunoassay using C terminal-directed developed gastrin/CCK antiserum. This assay method was detectable as little as 0.2 Pg CCKa/tube. These results that this method is applicable suggest assay simultaneously to determine both ACh and CCKLI output in the dialysate.
617
CHOLECYSTOKININ(CCK)INDUCESSHORT-LASTING EXCITATION INREPETITIVEFIRING NEURONS OF THE RAT NUCLEUS TRACTUS SOLITARIUS (NTS). KENJI SUZUKI’. SHUJI AOU’, NOBUAKI SHIMIZU2eAND, 'Deut. ofPhvsiol., Kvushu Univ.. Facultv of Medicine. Fukuoka 8 12, Jaoan. 2Deot. of Chem. & Chem. Ene.. Kanazawa Univ.. Kanazawa 920. Jauan.
The medial NTS (mNTS) neurons predominantly receive gastrointestinal inputs and possess CCKA receptors which mediate asatietysignal. We performed extracellularand intracellular recordings fromneurons of the mNTS in vitroto characterize their electrophysiological properties and CCK sensitivity. CCK (