- Email: [email protected]
Chromatographic methods
Jorrrrrnl of ClrrornNtr~~raplry. I 14 (1975) 302 0 Elscvicr Scientific Publishing Company, Amsterdam CHROM.
Book
-
Printed
in The Netherlands
8443
Review
tndto& by R. Stock and C. B. F. Rice, Science Paperbacks, Chapman & Hall, London, 3rd ed., 1974, VIII + 383 pp,, price f2.90 (paperback), f 5.25 (hardbound).
Cltrornacograpkic
The enlarged and revised third edition of this book provides a wealth of useful practical detail, although this is somewhat dated. It also illustrates the principles of the variety of chromatographic techniques including zone electrophorcsis. But it is astonishing that so many incorrect or misleading statements should be made throughout the body of the book. This means that the reader has to exercise extreme care in using this book for serious study. For example, it is stated on p. IS and again on p. 86 that “plate number gives a measure of the separating power of the column”. In fact plate number describes the bond dispersion which would be expected of a given column; it has no effect on the separation per SC’,this being a function of column selectivity. On p. 89 it is stated that because the pressure in a liquid chromatographic (LC) column is hydrostatic, little pressure drop is experienced across the column and hence the mobile phase linear velocity is virtually constant. In fact, the pressure drop across an LC column can be very high (6000 p.s.i.), but the reason for a nominally constant linear flow throughout the length of the column is that liquids are substantially incompressible up to the quoted figure. P. 91 states that LC columns can be up to 50-60 m in length. The reviewer knows of no work which has been done where the column has been longer than 5 m. The pressure drop required would be completely outside present day capability. The impression gained on reading this book is that little thought has been given to the organisation of the subject matter, so that the same material is reiterated in the various sections. The lack of adequate presentation of the material in the book and the numerous errors in fact make this book unsuitable for serious study. Briglttou
(Greaf
&itch)
C. F. SIMPSON
Book
-
Printed
in The Netherlands
8443
Review
tndto& by R. Stock and C. B. F. Rice, Science Paperbacks, Chapman & Hall, London, 3rd ed., 1974, VIII + 383 pp,, price f2.90 (paperback), f 5.25 (hardbound).
Cltrornacograpkic
The enlarged and revised third edition of this book provides a wealth of useful practical detail, although this is somewhat dated. It also illustrates the principles of the variety of chromatographic techniques including zone electrophorcsis. But it is astonishing that so many incorrect or misleading statements should be made throughout the body of the book. This means that the reader has to exercise extreme care in using this book for serious study. For example, it is stated on p. IS and again on p. 86 that “plate number gives a measure of the separating power of the column”. In fact plate number describes the bond dispersion which would be expected of a given column; it has no effect on the separation per SC’,this being a function of column selectivity. On p. 89 it is stated that because the pressure in a liquid chromatographic (LC) column is hydrostatic, little pressure drop is experienced across the column and hence the mobile phase linear velocity is virtually constant. In fact, the pressure drop across an LC column can be very high (6000 p.s.i.), but the reason for a nominally constant linear flow throughout the length of the column is that liquids are substantially incompressible up to the quoted figure. P. 91 states that LC columns can be up to 50-60 m in length. The reviewer knows of no work which has been done where the column has been longer than 5 m. The pressure drop required would be completely outside present day capability. The impression gained on reading this book is that little thought has been given to the organisation of the subject matter, so that the same material is reiterated in the various sections. The lack of adequate presentation of the material in the book and the numerous errors in fact make this book unsuitable for serious study. Briglttou
(Greaf
&itch)
C. F. SIMPSON