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Chromosomal alterations in a case of ganglioglioma
ELSEVIER
LETTERS TO THE EDITOR Chromosomal Alterations in a Case of Ganglioglioma
Gangliogliomas are low-grade primary CNS tumors composed of mixed populations of glial elements and an admixture of neuroblasts [1]. In this study, we present a 51-year-old woman, who underwent surgical resection of a cerebral tumor (2.5-×-3.0 -cm in size) of the right parietal region in June 1992. The patient remains asymptomatic. Based on histopathology and ultrastructure, the tumor was diagnosed as a ganglioglJoma (Fig. 1). Cytogenetic analysis of the tumor tissue was ]performed after short-term culturo Figure I Histopathology of tumor, a) Synaptophysin immunohistochemistry showing nuraerous immunopositive neuronal cells; × 100. b) Electronmicrograph of tumor cells showing a neuronal process containing abundant dense-core vesicles surrounded by astrocytic processes; × 12000.
ing, harvesting, and G-banding as previously described [2]. The subsequent karyotypic analysis and nomenclature met the criteria of the ISCN [3]. The karyotype 43,XX, der(1)t(1;5)(q21;q12),- 5,der(8;13)(q10;q10),- 9,i(10)(q10) was found in seven cells (Fig. 2), five cells displayed nonclonal chromosome changes, and the remaining 14 mitoses were normal. Gangliogliomas, clinically benign tumors of the complex entity, have been reported to display a predominantly normal karyotype [4-6}. In our tumor, the chromosomal changes display some similarity to those frequently seen in the malignant gliomas. Particularly, the changes leading to the partial or total loss of chromosomes 9 and 10 are frequently seen in tumors of astrocytic origin [7]. Loss of chromosome 10 precedes transition from benign astrocytoma to glioblastoma [8]. In addition, partial m o n o s o m y of chromosome 9 may also represent a chromosome change associated with tumor progression, but chromosome 9 abnormalities are not exclusive to high-grade astrocytic tumors [9]. Gangliogliomas are benign tumors but occasionally they undergo malignant transformation [1]. It is a glial component that can progress into malignant tumor. Since our patient remains asymptomatic, one may speculate that the loss of the chromosome 9 and the short arm of chromosome 10 (the consequence of isochromosome formation), were not critical for the progression of glial cells bearing these changes to the malignant ones. Follow-up of our patient is pending. This paper was supported by the research grant from the Polish State Research Committee. MARIA DEBIEC-RYCHTER PAWEL P. LIBERSKI JANUSZ ALWASIAK ANDRZEJ KLIMEK
Laboratory of Tumor Biology Department of Tumor Pathology, Medical Academy of Lodz, Department of Neurology, Regional Multidisciplinary Hospital (Copernicus Hospital), Lodz, Poland.
REFERENCES 1. Russel DC, Rubinstein LJ (1989): Pathology of Brain Tumors of
the Nervous system, 5th Ed. Edward Arnold, London, Melbourne, Auckland. 2. Debiec-Rychter M, Saryusz-Wolska H, Salagierski M (1992): Cytogenetic analysis of renal angiomyolipoma. Genes Chrom Cancer 4:101-103. Cancer Genet Cytogenet 85:155--156 (1995) © Elsevier Science Inc., 1995 655 Avenue of the Americas, New York, NY 10010
0165-4608/95/$9.50 SSDI 0165-4608(95)00146-G
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Figure 2 GTG-banded karyotype displaying three marker chromosomes: M1, der(1)t(1;5)(q21;q12); M2,der (8;13) (ql0;ql0); and M3, i(lO)(qlO).
3. ISCN (1991): Guidelines for Cancer Cytogenetics. Supplement to An International System for Human Cytogenetic Nomenclature, Mitelman F (ed), S. Karger, Basel. 4. Mitelman F (1991): Catalogue of Chromosome Aberrations in Cancer, 4th Ed. Alan R. Liss, New York. 5. Neumann E, Kalousek DK, Norman MG, Steinbok P, Cochrane DD, Goddard K (1993): Cytogenetic analysis of 109 pediatric central nervous system tumors. Cancer Genet Cytogenet 71:4049. 6. Vagner-Capodano AM, Gentet JC, Gamberelli D, Pellissier JF, Gouzian M, Lena G, Genitori L, Choux M, Raybaud C (1992):
Cytogenetic studies in 45 pediatric brain tumors. Pediatr Hematol Oncol 9:223-235. 7. Bigner SH, Vogelstein B (1990): Cytogenetics and molecular genetics of malignant gliomas and medulloblastomas. Brain Pathol 1:12-18. 8. Rasheed BKA, Bigner SH (1991): Genetic alterations in glioma and medulloblastoma. Cancer Met Rev 10:286-299. 9. Bello MJ, de Campos JM, Vaquero J, Kusak ME., Sarasa JL, Pestana A, Rey JA (1994): Molecular and cytogenetic analysis of chromosome 9 deletions in 75 malignant gliomas. Genes Chrom Cancer 9:33-41.
LETTERS TO THE EDITOR Chromosomal Alterations in a Case of Ganglioglioma
Gangliogliomas are low-grade primary CNS tumors composed of mixed populations of glial elements and an admixture of neuroblasts [1]. In this study, we present a 51-year-old woman, who underwent surgical resection of a cerebral tumor (2.5-×-3.0 -cm in size) of the right parietal region in June 1992. The patient remains asymptomatic. Based on histopathology and ultrastructure, the tumor was diagnosed as a ganglioglJoma (Fig. 1). Cytogenetic analysis of the tumor tissue was ]performed after short-term culturo Figure I Histopathology of tumor, a) Synaptophysin immunohistochemistry showing nuraerous immunopositive neuronal cells; × 100. b) Electronmicrograph of tumor cells showing a neuronal process containing abundant dense-core vesicles surrounded by astrocytic processes; × 12000.
ing, harvesting, and G-banding as previously described [2]. The subsequent karyotypic analysis and nomenclature met the criteria of the ISCN [3]. The karyotype 43,XX, der(1)t(1;5)(q21;q12),- 5,der(8;13)(q10;q10),- 9,i(10)(q10) was found in seven cells (Fig. 2), five cells displayed nonclonal chromosome changes, and the remaining 14 mitoses were normal. Gangliogliomas, clinically benign tumors of the complex entity, have been reported to display a predominantly normal karyotype [4-6}. In our tumor, the chromosomal changes display some similarity to those frequently seen in the malignant gliomas. Particularly, the changes leading to the partial or total loss of chromosomes 9 and 10 are frequently seen in tumors of astrocytic origin [7]. Loss of chromosome 10 precedes transition from benign astrocytoma to glioblastoma [8]. In addition, partial m o n o s o m y of chromosome 9 may also represent a chromosome change associated with tumor progression, but chromosome 9 abnormalities are not exclusive to high-grade astrocytic tumors [9]. Gangliogliomas are benign tumors but occasionally they undergo malignant transformation [1]. It is a glial component that can progress into malignant tumor. Since our patient remains asymptomatic, one may speculate that the loss of the chromosome 9 and the short arm of chromosome 10 (the consequence of isochromosome formation), were not critical for the progression of glial cells bearing these changes to the malignant ones. Follow-up of our patient is pending. This paper was supported by the research grant from the Polish State Research Committee. MARIA DEBIEC-RYCHTER PAWEL P. LIBERSKI JANUSZ ALWASIAK ANDRZEJ KLIMEK
Laboratory of Tumor Biology Department of Tumor Pathology, Medical Academy of Lodz, Department of Neurology, Regional Multidisciplinary Hospital (Copernicus Hospital), Lodz, Poland.
REFERENCES 1. Russel DC, Rubinstein LJ (1989): Pathology of Brain Tumors of
the Nervous system, 5th Ed. Edward Arnold, London, Melbourne, Auckland. 2. Debiec-Rychter M, Saryusz-Wolska H, Salagierski M (1992): Cytogenetic analysis of renal angiomyolipoma. Genes Chrom Cancer 4:101-103. Cancer Genet Cytogenet 85:155--156 (1995) © Elsevier Science Inc., 1995 655 Avenue of the Americas, New York, NY 10010
0165-4608/95/$9.50 SSDI 0165-4608(95)00146-G
156
M. Debiec-Rychter et al.
6
13
19
7
14
s M2
9
M3
16
15
20
10
21
11
12
18
17
22
××
Figure 2 GTG-banded karyotype displaying three marker chromosomes: M1, der(1)t(1;5)(q21;q12); M2,der (8;13) (ql0;ql0); and M3, i(lO)(qlO).
3. ISCN (1991): Guidelines for Cancer Cytogenetics. Supplement to An International System for Human Cytogenetic Nomenclature, Mitelman F (ed), S. Karger, Basel. 4. Mitelman F (1991): Catalogue of Chromosome Aberrations in Cancer, 4th Ed. Alan R. Liss, New York. 5. Neumann E, Kalousek DK, Norman MG, Steinbok P, Cochrane DD, Goddard K (1993): Cytogenetic analysis of 109 pediatric central nervous system tumors. Cancer Genet Cytogenet 71:4049. 6. Vagner-Capodano AM, Gentet JC, Gamberelli D, Pellissier JF, Gouzian M, Lena G, Genitori L, Choux M, Raybaud C (1992):
Cytogenetic studies in 45 pediatric brain tumors. Pediatr Hematol Oncol 9:223-235. 7. Bigner SH, Vogelstein B (1990): Cytogenetics and molecular genetics of malignant gliomas and medulloblastomas. Brain Pathol 1:12-18. 8. Rasheed BKA, Bigner SH (1991): Genetic alterations in glioma and medulloblastoma. Cancer Met Rev 10:286-299. 9. Bello MJ, de Campos JM, Vaquero J, Kusak ME., Sarasa JL, Pestana A, Rey JA (1994): Molecular and cytogenetic analysis of chromosome 9 deletions in 75 malignant gliomas. Genes Chrom Cancer 9:33-41.