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CLINICAL APPLICATION OF A SOFTWARE DEVELOPED FOR OBTAINING ELECTROCARDIOGRAPHIC SIGNALS
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known coronary artery disease (67%), hypertension (83%); diabetes mellitus (65%), and past or current cigarette smoking (32%). The mean HbA1c ¼ 7.0%. Majority of the patients (92.0%) were taking statins. The mean cholesterol levels were: Total cholesterol 4.2 mmol/L, LDL-C 2.1 mmol/L, HDL-C 1.3 mmol/L, non-HDL-C 2.6 mmol/L and triglyceride of 1.6 mmol/L. The mean Apo B levels was 0.8 g/L. The table below shows the correlation coefficients for Apo B and each of the cholesterol parameters. In patients with diabetes mellitus the correlation coefficient for Apo B and LDL-C was 0.92. Comparison was also made between males and females, and there was no difference between the two groups with respect to Apo B and LDL-C.
CONCLUSION:
In SA with a high prevalence of coronary artery disease and statin use, Apo B correlated strongly with LDL-C and non-HDL-C levels. A similar high level of correlation was seen between Apo B and LDL-C in patients with diabetes mellitus. Our findings further extend the current knowledge of the relationship between Apo B and LDL-C to include South Asians, a population at high risk for atherosclerosis and underrepresented in clinical trails.
052 CLINICAL APPLICATION OF A SOFTWARE DEVELOPED FOR OBTAINING ELECTROCARDIOGRAPHIC SIGNALS
Canadian Journal of Cardiology Volume 30 2014
steps: 1. the image is converted to grayscale; 2. the background image is removed by thresholding; 3. the waveform is obtained by converting the values of pixels in an arbitrary amplitude scale; 4. interpolation is applied to the obtained signal in order to ensure there are no gaps in the signal; 5. the signal is normalized and sampled in accordance with parameters pre-set by the user. Printed electrocardiograms must be first converted into digital images for further manipulation in the program. The validation of this method was carried out with ECG records of 40 patients. The printed ECGs were scanned and electrocardiographic signals obtained from the images. It was compared the measurements of the manual values obtained to P wave duration, QRS duration and T wave duration in the printed ECG and the values of the obtained digital ECG. It was performed the t-test for a sample mean to compare the values; obtained manually and those obtained from the developed software. The obtained results showed that the developed software is a useful and relevant tool for the reconstruction of digital ECG from printed waveforms. It was obtained a p-value greater than 0.05 (p > 0.05) and the null hypothesis was accepted, therefore the statistical analysis, comparing estimates from digital ECG and manual measurements, suggested no relevant differences in the estimate of the duration of the P wave, QRS interval and T wave. CONCLUSION: The developed software is an efficient method for obtaining electrocardiographic signals and it can be a useful application for clinical use, cardiovascular researches and signal processing.
053 CELASTROL PREVENTS ADVERSE CARDIAC REMODELING OF THE ISCHEMIC MYOCARDIUM S DerSarkissian, J Cailhier, M Borie, L Stevens, L Gaboury, S Mansour, N Noiseux
L Resende, A Andrade, E Resende
Montréal, Québec
Uberlândia, Brazil
BACKGROUND:
BACKGROUND:
The electrocardiogram (ECG) is widely used in diagnosis, planning of cardiovascular therapeutic intervention and evaluation of heart diseases. The aim of this study is to present clinical application and validation of a software developed for obtaining electrocardiographic signals from printed ECGs. The relevance of the developed software is related to the importance of free tools to obtain electrocardiographic signs, especially as a method to support researches, in order to allow the computational processing, the development of tools and Methods for analysis of these signals. This research presents the software development and the applications related to its use in clinical practice. METHOD AND RESULTS: The software for obtaining electrocardiographic signals was developed in Matlab, it has a graphical interface for rapid and easy use by health professionals. General steps of image processing to extract the waveform of the electrocardiogram consist of the following
We reported that Celastrol a natural product from the root bark of a traditional oriental medicinal plant “Thunder God Vine” triggers a heat shock response in rat cardiomyoblast cells with nuclear translocation and activation of transcription factor HSF1, leading to upregulation of several cardioprotective heat shock proteins (HSP) including HO-1 and HSP70. Celastrol induced a rapid activation of PI3K/Akt and MAPK signaling pathways, which were partially dependent on radical oxygen species (ROS). The upregulation of HSP was dependent of HSF1 and ROS signaling. Functional analysis demonstrated a dose-response protection against cell death during hypoxia and oxidative stress involving HSF1/HO-1 pathways. The objective hereby is to characterize the beneficial effects of Celastrol in ischemic myocardium and determine the safety of the treatment regimen. METHODS AND RESULTS: Sprague-Dawley rats underwent myocardial infarct (MI) by coronary artery occlusion, and
known coronary artery disease (67%), hypertension (83%); diabetes mellitus (65%), and past or current cigarette smoking (32%). The mean HbA1c ¼ 7.0%. Majority of the patients (92.0%) were taking statins. The mean cholesterol levels were: Total cholesterol 4.2 mmol/L, LDL-C 2.1 mmol/L, HDL-C 1.3 mmol/L, non-HDL-C 2.6 mmol/L and triglyceride of 1.6 mmol/L. The mean Apo B levels was 0.8 g/L. The table below shows the correlation coefficients for Apo B and each of the cholesterol parameters. In patients with diabetes mellitus the correlation coefficient for Apo B and LDL-C was 0.92. Comparison was also made between males and females, and there was no difference between the two groups with respect to Apo B and LDL-C.
CONCLUSION:
In SA with a high prevalence of coronary artery disease and statin use, Apo B correlated strongly with LDL-C and non-HDL-C levels. A similar high level of correlation was seen between Apo B and LDL-C in patients with diabetes mellitus. Our findings further extend the current knowledge of the relationship between Apo B and LDL-C to include South Asians, a population at high risk for atherosclerosis and underrepresented in clinical trails.
052 CLINICAL APPLICATION OF A SOFTWARE DEVELOPED FOR OBTAINING ELECTROCARDIOGRAPHIC SIGNALS
Canadian Journal of Cardiology Volume 30 2014
steps: 1. the image is converted to grayscale; 2. the background image is removed by thresholding; 3. the waveform is obtained by converting the values of pixels in an arbitrary amplitude scale; 4. interpolation is applied to the obtained signal in order to ensure there are no gaps in the signal; 5. the signal is normalized and sampled in accordance with parameters pre-set by the user. Printed electrocardiograms must be first converted into digital images for further manipulation in the program. The validation of this method was carried out with ECG records of 40 patients. The printed ECGs were scanned and electrocardiographic signals obtained from the images. It was compared the measurements of the manual values obtained to P wave duration, QRS duration and T wave duration in the printed ECG and the values of the obtained digital ECG. It was performed the t-test for a sample mean to compare the values; obtained manually and those obtained from the developed software. The obtained results showed that the developed software is a useful and relevant tool for the reconstruction of digital ECG from printed waveforms. It was obtained a p-value greater than 0.05 (p > 0.05) and the null hypothesis was accepted, therefore the statistical analysis, comparing estimates from digital ECG and manual measurements, suggested no relevant differences in the estimate of the duration of the P wave, QRS interval and T wave. CONCLUSION: The developed software is an efficient method for obtaining electrocardiographic signals and it can be a useful application for clinical use, cardiovascular researches and signal processing.
053 CELASTROL PREVENTS ADVERSE CARDIAC REMODELING OF THE ISCHEMIC MYOCARDIUM S DerSarkissian, J Cailhier, M Borie, L Stevens, L Gaboury, S Mansour, N Noiseux
L Resende, A Andrade, E Resende
Montréal, Québec
Uberlândia, Brazil
BACKGROUND:
BACKGROUND:
The electrocardiogram (ECG) is widely used in diagnosis, planning of cardiovascular therapeutic intervention and evaluation of heart diseases. The aim of this study is to present clinical application and validation of a software developed for obtaining electrocardiographic signals from printed ECGs. The relevance of the developed software is related to the importance of free tools to obtain electrocardiographic signs, especially as a method to support researches, in order to allow the computational processing, the development of tools and Methods for analysis of these signals. This research presents the software development and the applications related to its use in clinical practice. METHOD AND RESULTS: The software for obtaining electrocardiographic signals was developed in Matlab, it has a graphical interface for rapid and easy use by health professionals. General steps of image processing to extract the waveform of the electrocardiogram consist of the following
We reported that Celastrol a natural product from the root bark of a traditional oriental medicinal plant “Thunder God Vine” triggers a heat shock response in rat cardiomyoblast cells with nuclear translocation and activation of transcription factor HSF1, leading to upregulation of several cardioprotective heat shock proteins (HSP) including HO-1 and HSP70. Celastrol induced a rapid activation of PI3K/Akt and MAPK signaling pathways, which were partially dependent on radical oxygen species (ROS). The upregulation of HSP was dependent of HSF1 and ROS signaling. Functional analysis demonstrated a dose-response protection against cell death during hypoxia and oxidative stress involving HSF1/HO-1 pathways. The objective hereby is to characterize the beneficial effects of Celastrol in ischemic myocardium and determine the safety of the treatment regimen. METHODS AND RESULTS: Sprague-Dawley rats underwent myocardial infarct (MI) by coronary artery occlusion, and