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Clinicopathological Significance of MMP-25 Immunoexpression in Oral Carcinogenesis
ORAL AND MAXILLOFACIAL PATHOLOGY e202 Abstracts desmoplastic, and 2 (3%) peripheral ameloblastomas. The posterior (74%) and anterior (13%) mandible were the sites most often affected. The mean size of the radiological images was 45.9 mm, and they were unilocular in 42% and multilocular in 53% of the cases. Follicular and plexiform subtypes were the two most common histological patterns. Conclusion: Clinicopathological characteristics of this sample were similar to the most common findings worldwide. Financial support: FAPERJ.
PE-362 - CLINICOPATHOLOGICAL SIGNIFICANCE OF MMP-25 IMMUNOEXPRESSION IN ORAL CARCINOGENESIS. REBECA BARROS NASCIMENTO, FERNANDA CAIRES BORBA, LORENA AGUIAR SANTOS FARIA, LIA PONTES ARRUDA PORTO, KATIÚCIA BATISTA SILVA PAIVA, JEAN NUNES DOS SANTOS, FLÁVIA CALÓ DE AQUINO XAVIER. UNIVERSIDADE FEDERAL DA BAHIA. Biological markers to improve diagnosis and prognosis may be a valuable tool. MMP-25 is a GPI-anchored MMP on the cell membrane. Its role in cancer remains unclear. This study evaluated MMP-25 immunoexpression in oral carcinogenesis. Study Design: Immunohistochemical analysis for MMP-25 was performed in 13 normal oral mucosa (NOM), 14 oral epithelial dysplasia (OED), and 24 oral squamous cell carcinoma (OSCC) formalin-fixed paraffin-embedded specimens. Clinicopathological data (age, gender, smoking habit, site, duration, and pathological grade) were also evaluated. Results: All NOM showed MMP-25 immunoexpression. Both OEDs (n ¼ 8/14) and OSCCs (n ¼ 19/ 24) demonstrated immunoexpression predominantly distributed in strong and moderate nuclear/cytoplasmatic staining. There was a trend toward MMP-25 immunoexpression in OSCC patients over age 60 years (p ¼ 0.15, Fisher exact test), and female patients (p ¼ 0.13) with hyperkeratosis (p ¼ 0.07). Conclusion: MMP-25 may participate in some lesion phenotypes, but additional studies are needed to better understand its participation in oral carcinogenesis.
PE-363 - COMPARATIVE STUDY BETWEEN PLEOMORPHIC ADENOMA AND CARCINOMA EX-PLEOMORPHIC ADENOMA BY ARRAY-COMPARATIVE GENOMIC HYBRIDIZATION. FERNANDA VIVIANE MARIANO, ROGÉRIO DE OLIVEIRA GONDAK, LUIZ PAULO KOWALSKI, RICARDO DELLA COLETTA, OSLEI PAES DE ALMEIDA, ANA CRISTINA VICTORINO KREPISCHI, ALBINA ALTEMANI. FCM-UNICAMP, FOP-UNICAMP, HOSPITAL AC CAMARGO, CIPE-AC CAMARGO. The comparison between pleomorphic adenoma (PA) and carcinoma ex-pleomorphic adenoma (CXPA) can be important in understanding the malignant transformation process. Materials and Methods. Five PAs and eight CXPAs were evaluated by array-comparative genomic hybridization (array-CGH). Results: The PAs showed a prevalence of gain in chromosome 8 and loss in 17q, 19q. All alterations in PAs were maintained in CXPAs, with additional gains and losses. A different genomic profile was found in all CXPAs. More changes did not occur with increased tumor invasiveness. The most-often repeated alterations in CXPAs were gains and losses in chromosomes 2, 5, and 8. Conclusion: In comparison to PAs, CXPAs showed an increase of genomic alterations. The changes in chromosomes 2 and 5 may be important for malignant transformation. It was impossible to identify a particular genotypic pattern for each histological subtype and stage of carcinogenesis. However, more cases need to be investigated.
OOOO February 2014 PE-364 - COMPARATIVE STUDY OF CD8+ LYMPHOCYTES IN SALIVARY GLANDS AND LIVER OF PATIENTS WITH CHRONIC HEPATITIS C. KARLA RACHEL OLIVEIRA E SILVA, PATRÍCIA CARLOS CALDEIRA, PAULA VIEIRA TEIXEIRA VIDIGAL, MARIA AUXILIADORA VIEIRA DO CARMO. FACULDADE DE ODONTOLOGIA DA UNIVERSIDADE FEDERAL DE MINAS GERAIS.
This study compared the percentage of CD8+ lymphocytes in the salivary glands and liver of patients with chronic hepatitis C, considering the distribution pattern of inflammation. Methods: Sixty-one samples of salivary glands and 59 of liver were tested immunohistochemically for CD8+. Infiltrate was classified as diffuse or focal. Results: CD8+ levels were higher in liver than in salivary glands. Salivary glands showed higher CD8+ infiltration within focal infiltrate than in diffuse infiltrate, but a lower value than liver with focal infiltrate. Salivary glands with diffuse infiltrate presented a lower value than liver with diffuse infiltrate. Liver with diffuse infiltrate showed a slightly higher index than liver with focal infiltrate. Comparison of the salivary glands with positive and negative HCV RNA showed no statistical differences. Conclusion: CD8+ lymphocytes are found in salivary glands in a lower proportion than in liver, but their presence did not depend on the local presence of HCV RNA. Support: FAPEMIG.
PE-365 - COMPARATIVE STUDY OF STYLOID LIGAMENT OSSIFICATION AMONG PATIENTS SUBJECT TO TONSILLECTOMY. ORLANDO IZOLANI NETO, MARCUS VINICIUS DE SOUZA, ANAMARIA DE LIMA LARANJEIRA, MILENA BORTOLOTTO FELIPPE SILVA, SILVIA CRISTINA MAZETI TORRES, JOSÉ LUIZ CINTRA JUNQUEIRA, CAIO VINÍCIUS BARDI MATAI. CPO - SÃO LEOPOLDO MANDIC. Styloid ligament ossification is a common finding when evaluating radiographic images. This study established an epidemiological profile of styloid ligament ossification from the observation of panoramic radiographs in patients undergoing tonsillectomy and compared them with those of patients not having this intervention. The sample included 200 patient radiographs considered to show styloid ossified ligaments that extended beyond the anatomic location of the earlobe. A total of 170 patients reported not having undergone tonsillectomy. Of these, 25.3% had ligament ossification, 69.7% had bilateral cases, and 67.4% were from female patients. The remaining 30 radiographs were from patients with a history of palatal tonsil removal, and 26.6% showed ossification, all bilateral; 62.5% were from male patients. Ossification was found on an average of 25% of the images assessed. There were no differences between patients who underwent surgery to remove the tonsils and those who did not undergo this intervention.
PE-366 - CORRELATION BETWEEN SALIVARY ANTIHCV ANTIBODIES AND HCV RNA IN SALIVA AND SALIVARY GLANDS OF PATIENTS WITH CHRONIC HEPATITIS C. PATRÍCIA CARLOS CALDEIRA, KARLA RACHEL OLIVEIRA E SILVA, TARCÍLIA APARECIDA SILVA, MARIA AUXILIADORA VIEIRA DO CARMO. FACULDADE DE ODONTOLOGIA DA UNIVERSIDADE FEDERAL DE MINAS GERAIS. This study investigated the correlation between anti-HCV antibodies in saliva and HCV RNA in saliva and salivary glands of patients with chronic hepatitis C. Methods: A total of 131
PE-362 - CLINICOPATHOLOGICAL SIGNIFICANCE OF MMP-25 IMMUNOEXPRESSION IN ORAL CARCINOGENESIS. REBECA BARROS NASCIMENTO, FERNANDA CAIRES BORBA, LORENA AGUIAR SANTOS FARIA, LIA PONTES ARRUDA PORTO, KATIÚCIA BATISTA SILVA PAIVA, JEAN NUNES DOS SANTOS, FLÁVIA CALÓ DE AQUINO XAVIER. UNIVERSIDADE FEDERAL DA BAHIA. Biological markers to improve diagnosis and prognosis may be a valuable tool. MMP-25 is a GPI-anchored MMP on the cell membrane. Its role in cancer remains unclear. This study evaluated MMP-25 immunoexpression in oral carcinogenesis. Study Design: Immunohistochemical analysis for MMP-25 was performed in 13 normal oral mucosa (NOM), 14 oral epithelial dysplasia (OED), and 24 oral squamous cell carcinoma (OSCC) formalin-fixed paraffin-embedded specimens. Clinicopathological data (age, gender, smoking habit, site, duration, and pathological grade) were also evaluated. Results: All NOM showed MMP-25 immunoexpression. Both OEDs (n ¼ 8/14) and OSCCs (n ¼ 19/ 24) demonstrated immunoexpression predominantly distributed in strong and moderate nuclear/cytoplasmatic staining. There was a trend toward MMP-25 immunoexpression in OSCC patients over age 60 years (p ¼ 0.15, Fisher exact test), and female patients (p ¼ 0.13) with hyperkeratosis (p ¼ 0.07). Conclusion: MMP-25 may participate in some lesion phenotypes, but additional studies are needed to better understand its participation in oral carcinogenesis.
PE-363 - COMPARATIVE STUDY BETWEEN PLEOMORPHIC ADENOMA AND CARCINOMA EX-PLEOMORPHIC ADENOMA BY ARRAY-COMPARATIVE GENOMIC HYBRIDIZATION. FERNANDA VIVIANE MARIANO, ROGÉRIO DE OLIVEIRA GONDAK, LUIZ PAULO KOWALSKI, RICARDO DELLA COLETTA, OSLEI PAES DE ALMEIDA, ANA CRISTINA VICTORINO KREPISCHI, ALBINA ALTEMANI. FCM-UNICAMP, FOP-UNICAMP, HOSPITAL AC CAMARGO, CIPE-AC CAMARGO. The comparison between pleomorphic adenoma (PA) and carcinoma ex-pleomorphic adenoma (CXPA) can be important in understanding the malignant transformation process. Materials and Methods. Five PAs and eight CXPAs were evaluated by array-comparative genomic hybridization (array-CGH). Results: The PAs showed a prevalence of gain in chromosome 8 and loss in 17q, 19q. All alterations in PAs were maintained in CXPAs, with additional gains and losses. A different genomic profile was found in all CXPAs. More changes did not occur with increased tumor invasiveness. The most-often repeated alterations in CXPAs were gains and losses in chromosomes 2, 5, and 8. Conclusion: In comparison to PAs, CXPAs showed an increase of genomic alterations. The changes in chromosomes 2 and 5 may be important for malignant transformation. It was impossible to identify a particular genotypic pattern for each histological subtype and stage of carcinogenesis. However, more cases need to be investigated.
OOOO February 2014 PE-364 - COMPARATIVE STUDY OF CD8+ LYMPHOCYTES IN SALIVARY GLANDS AND LIVER OF PATIENTS WITH CHRONIC HEPATITIS C. KARLA RACHEL OLIVEIRA E SILVA, PATRÍCIA CARLOS CALDEIRA, PAULA VIEIRA TEIXEIRA VIDIGAL, MARIA AUXILIADORA VIEIRA DO CARMO. FACULDADE DE ODONTOLOGIA DA UNIVERSIDADE FEDERAL DE MINAS GERAIS.
This study compared the percentage of CD8+ lymphocytes in the salivary glands and liver of patients with chronic hepatitis C, considering the distribution pattern of inflammation. Methods: Sixty-one samples of salivary glands and 59 of liver were tested immunohistochemically for CD8+. Infiltrate was classified as diffuse or focal. Results: CD8+ levels were higher in liver than in salivary glands. Salivary glands showed higher CD8+ infiltration within focal infiltrate than in diffuse infiltrate, but a lower value than liver with focal infiltrate. Salivary glands with diffuse infiltrate presented a lower value than liver with diffuse infiltrate. Liver with diffuse infiltrate showed a slightly higher index than liver with focal infiltrate. Comparison of the salivary glands with positive and negative HCV RNA showed no statistical differences. Conclusion: CD8+ lymphocytes are found in salivary glands in a lower proportion than in liver, but their presence did not depend on the local presence of HCV RNA. Support: FAPEMIG.
PE-365 - COMPARATIVE STUDY OF STYLOID LIGAMENT OSSIFICATION AMONG PATIENTS SUBJECT TO TONSILLECTOMY. ORLANDO IZOLANI NETO, MARCUS VINICIUS DE SOUZA, ANAMARIA DE LIMA LARANJEIRA, MILENA BORTOLOTTO FELIPPE SILVA, SILVIA CRISTINA MAZETI TORRES, JOSÉ LUIZ CINTRA JUNQUEIRA, CAIO VINÍCIUS BARDI MATAI. CPO - SÃO LEOPOLDO MANDIC. Styloid ligament ossification is a common finding when evaluating radiographic images. This study established an epidemiological profile of styloid ligament ossification from the observation of panoramic radiographs in patients undergoing tonsillectomy and compared them with those of patients not having this intervention. The sample included 200 patient radiographs considered to show styloid ossified ligaments that extended beyond the anatomic location of the earlobe. A total of 170 patients reported not having undergone tonsillectomy. Of these, 25.3% had ligament ossification, 69.7% had bilateral cases, and 67.4% were from female patients. The remaining 30 radiographs were from patients with a history of palatal tonsil removal, and 26.6% showed ossification, all bilateral; 62.5% were from male patients. Ossification was found on an average of 25% of the images assessed. There were no differences between patients who underwent surgery to remove the tonsils and those who did not undergo this intervention.
PE-366 - CORRELATION BETWEEN SALIVARY ANTIHCV ANTIBODIES AND HCV RNA IN SALIVA AND SALIVARY GLANDS OF PATIENTS WITH CHRONIC HEPATITIS C. PATRÍCIA CARLOS CALDEIRA, KARLA RACHEL OLIVEIRA E SILVA, TARCÍLIA APARECIDA SILVA, MARIA AUXILIADORA VIEIRA DO CARMO. FACULDADE DE ODONTOLOGIA DA UNIVERSIDADE FEDERAL DE MINAS GERAIS. This study investigated the correlation between anti-HCV antibodies in saliva and HCV RNA in saliva and salivary glands of patients with chronic hepatitis C. Methods: A total of 131