Coagulation and colon cancer

287 Vestibular

disorders

in primary

Thrombocytemia

Famri B.‘, DC Gccio M.‘, Casani A.‘. Casahno P.‘. Sagripanll A?, Chilardi P.L.’ I ClinIca Ominolaringoiavica,

Univcrsila di Piu

2 Uniti Opcnliva di Emalolagia, Univcniti

Dtizziness and vertigo represent some of the most frequent symptoms in patients suffering from thrombocytemia. Origin and causes of such symptoms are not yet well established. In our study we evaluated Ihe state of vwibular system in 33 patients who had been suffering from thrombocytemia for a period ranging in time from I month up to 14 years. Nineteen patients showed central involvement signs:

hyperreflexia (Vm=609sec),

with remarkable increase smooth pursuit

magnitude=15.7“/sec,

Vm

gak0.78.

in

angular

impairments Vm THD=34.1%)

velocity (Vm and

pathological voluntary saccades. Seven patients showed peripheral involvement signs (in 3 cases we observed vestibular paresis and 4 cases presented vestibular

hyporeflexia).

The examination of ves~ibular spinal reflex by means of posturography mainly found signs of central involvement (increased surface of statokinesigram). Otoneurological

evaluation

showed

perceptive

hypoacousia

in cochlear seat. A high incidence of Central Vestibular impairments might be explained on the basis of an increased availability of serotonine (5HT) which does not depend on patient’s pisstrinosis, but occurs because of platelets activation. Infacts. 5HT represents an important neurotransmitter in cerebellar medial and inferior vestibular nuclei.

POLYCYTHEMIA VERA AND RECOMBINANT a2aINTERFERON : CLINICAL AND BIOLOGICAL EVALUATION BY MEANS OF FOURIER-TRANSFORM-INFRARED MICROSPECTROSCOPY. PAPINESCHI ‘-E , BUCALOSSI 2 A , CAPOCHIANI ’ E , BENEDElTl ’ E , BRAMANTI 3 E , G , DISPENSA 2 E BENEDElTl 3 E , SPREMOLLA 1 G .l Hematology Unit, i st Medical Chnic University of Pisa , Italy.2 Hematology Department , University of Siena , Italy.3 Laboratory of Infrared Spectroscopy, Department of Chemistry , University of Plsa,Ilaly. Polycythemia Vera ( PV ) is a chronic myeloproliferative disease. The chemotherapy and especially 32P although have a good conlrol of the disease , unfortunately determines an increased risk of leukemic transformation The rewmbinanl u2a Interferon ( IFN) therapy in this disease is a new and original approach. The eleven cases that we evaluated represent the largest casuisbc nowadays evaluable and with the longest period of follow-up performed. For the first time we applied a spectroscopic parameter ( A1 I A2 ). that is , the ratio of integrated areas of bands at 1080 cm-l and at 1540 cm1 due to DNA and proteic components respectively , calculated on the spectra of single megakaryocyles (MKs) In previous studies , we have pointed out that MKs in PV have a surprisingly strong myeloproliferative impulse when compared lo MKs from other chronic myeloproliferative diseases. Nine patients out of eleven showed a good clinical data response and red mass cell control with IFN treatment Eight patients had a good accordance of this parameter with the laboratory data. The infrared parameter by us proposed results to be an original and sure approach for the molecular evaluation of the recombinant a2a IFN responsiveness of these diseases

COAGULATION AND COLON CANCER Marcello

Camici,Oaniela

Barsacchi

2nd Medical Clinic.Clinical Fisiology ano Biochemistry.Pisa

and Lorenro

Evangelisti

Methodology and Department University. Italy.

of

The specificity of reactions between neoplasia and coagulation system remains uncertain.The aim of this stuay is to investigate the mechanism of coagulation activation oy neoplastic cells.Normel titrated plasma uas incubated with preparations of tissue extract(norma and pathological 30 )rl)obtained from patients operated for colon canter in order to determine their influence on the recalcification time(Houel1 Time/seconds.CaC12/0.02 rl)and platelet aggregation(Born method) induced by ADP/S pM.The tissue was cooled in liquid nitrogen and powdered in Microdismembra tor(8rauoMelsungen.Germany)at 50 vibrations/seconds.The tissue powder was suspended in Tris-HCl pH 7.4;homogeinizea by Potter-Elvhym homogenizer ano centrifuged (d°C) at 3O.uUO g/45 minutes.The surnatant was keepea at -80°C ana unfreezed just as use.Every experience yes repeated in

aup1icate.Afte.r is significantly (from

la c 0.M 2.3liO.31) 0.z) (-;-I INO I- ; -I InP:~*.m:cno:~o.a5: B% D 4.0 6.% Il.111 4.86 iO.S4-1.14116:% il3:3D-15301

0

I 03

0.31 i - : - I

22:Q .I - ; - 1. 1” L)(O.6;1 n C:,%rn:

ff w

I 0.01 6.37 lZ.nl6.s (0.~l.Wl c 0.06 3.43 Il.661 0.S ( - ; - I

e.oJ aai

14~38(lZ5t1):011 In D$O.m;ln C$O.& II:44 ( - ; - I I II D.pM.0:C I bpa.6;

W 0 @[email protected] ll.LO 0.A IO&1.181 1%46(1I:Yl):ll M Tka Qk idiak lkL aigitiml c@a. RI&~ k clkial m. PI !J tad W cimdiu rb)th aams ia B ptiats. lb @e shift in tb Bii t%7tb 1041k

98.6+3.6

5 minutes

of

incubation

Howell

Tima

by pathological tissue extract secondslbut not by normal tisaggregation is not affected by norm

decreased to 59.6+6.2

sue extracf.Platelet al or pathological tissue.Standard heparin(Liquemin.Roche MUZlS.OUU.Final concentration 0.5 U/ml)sianificantlv prolongs HT shortened by pathologieal~tiSSu~ extra&jrom 594.2 to 125.5+21.9 secondslbut pentosanpolysulphate (Sanoii Recherche.~oulouse.France.MW~3.700.Final concentration 300 ug/ml)doesn’t prolong it.These results suggest that ilT decrease obtsined by incubation with neoplastic tissue(procoagulant activity)is sustained by direct prothrombin activators.ln fact the high affinity of hepa rin fro antithrombin III prolongs HT shortened and the pentasaccharide of synthesis(pentosanpolysulphate)without affinity for antithrombin 111,doesn’t prolong HT shortened.Further efforts to characterize the activator of coagulation with activity-activating prothrombin and clarify biological and clinical significance appear to be necessary.