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Collagen based scaffold for biomedical applications
Special Abstracts / Journal of Biotechnology 150S (2010) S1–S576
[C.7] Collagen based scaffold for biomedical applications Monica Sandri 1,∗ , Anna Tampieri 1 , Luca Salvatore 2 , Alessandro Sannino 2 , Jung Hee Levialdi Ghiron 3 , Gianluigi Condorelli 3 1
Institute of Science and Technology for Ceramics, National Research Council, Italy 2 Department of Innovation Engineering, University of Lecce, Italy 3 Institute of Biomedical Technologies, National Research Council, Italy Keywords: Collagen; Biomimetic scaffold; Spin casting; Biomedical applications Next generation of biomaterials with enhanced functionalities requires the integration of material science and molecular biology, in order to identify bio-inspired designing concepts. New families of collagen based 3D scaffolds with biomimetic chemical-physical properties were obtained thanks to complex fabrication techniques able to generate specific geometries and pore structures in compliance with the specific regenerating tissue. Collagen is a significant constituent of the natural extracellular matrix (ECM) thus collagenous scaffolds have been used extensively in a variety of tissue engineering applications for their useful properties: hemostatic, low antigenicity, appropriate mechanical stiffness, promotion of cell and tissue attachment and growth. The remarkable biofunctional properties of tissues such as tendons/ligaments, depend on their hierarchic structures which is an organized assembly of structural units at increasing size levels. Tendon substitutes were prepared thanks to a special casting technique able to produce hole cylinders with controlled pores direction and dimensions. A pH-controlled fibration process using reinforcing natural nano-fibers was applied to obtain an artificial collagenous supramolecular assembly and appropriate mechanical properties. The process requires: i) the optimization of homogeneous and stable slurries and ii) the selection of effective operative parameters. Reticulation techniques were also employed to modifying the collagen surfaces and to achieve good resistance to physiological environment.
S29
Regenerative medicine has been also shown to be a potential alternative to cardiac transplantation for patients with severe heart failure. In this view a modified tape-casting technique was used to obtain thin preformed collagenous scaffolds/membranes to be tested as a potential vector for the transplantation of cardiomyocytes. Indeed the efficacy of the scaffolds endowed with contracting cardiac cell sheets was evaluated after application onto the injured area of rat infarcted myocardium in order to improve cardiac function. doi:10.1016/j.jbiotec.2010.08.084 [C.8] Drug profiling and biomarker discovery spectrometry-based proteomics technologies
using
mass
A. Jenne ∗ , A. Tebbe, C. Schaab, K. Godl, M. Kaminski, S. Müller KINAXO Biotechnologies GmbH, Germany Keywords: Phosphoproteomics; Chemical proteomics; Biomarker; Targeted drugs System-wide approaches integrating drug target identification and global phosphoproteome analysis provide detailed insights into the cellular mode of action of targeted cancer drugs. Proteome labeling methods such as stable isotope labeling by amino acids in cell culture (SILAC) are employed for quantitative mass spectrometry analysis of cell line and tissue samples. Combining these methods with state-of-the-art chemical proteomics technologies help reveal a compound’s protein affinities and its impact on signal transduction pathways. KINAXO has applied this approach to sorafenib (NexavarTM , Bayer Healthcare), a targeted drug approved for the treatment of kidney and liver cancer. Our findings suggest a previously unpublished mode of action for sorafenib through inhibition of the mTOR pathway in prostate cancer cells (PC-3).
doi:10.1016/j.jbiotec.2010.08.085
[C.7] Collagen based scaffold for biomedical applications Monica Sandri 1,∗ , Anna Tampieri 1 , Luca Salvatore 2 , Alessandro Sannino 2 , Jung Hee Levialdi Ghiron 3 , Gianluigi Condorelli 3 1
Institute of Science and Technology for Ceramics, National Research Council, Italy 2 Department of Innovation Engineering, University of Lecce, Italy 3 Institute of Biomedical Technologies, National Research Council, Italy Keywords: Collagen; Biomimetic scaffold; Spin casting; Biomedical applications Next generation of biomaterials with enhanced functionalities requires the integration of material science and molecular biology, in order to identify bio-inspired designing concepts. New families of collagen based 3D scaffolds with biomimetic chemical-physical properties were obtained thanks to complex fabrication techniques able to generate specific geometries and pore structures in compliance with the specific regenerating tissue. Collagen is a significant constituent of the natural extracellular matrix (ECM) thus collagenous scaffolds have been used extensively in a variety of tissue engineering applications for their useful properties: hemostatic, low antigenicity, appropriate mechanical stiffness, promotion of cell and tissue attachment and growth. The remarkable biofunctional properties of tissues such as tendons/ligaments, depend on their hierarchic structures which is an organized assembly of structural units at increasing size levels. Tendon substitutes were prepared thanks to a special casting technique able to produce hole cylinders with controlled pores direction and dimensions. A pH-controlled fibration process using reinforcing natural nano-fibers was applied to obtain an artificial collagenous supramolecular assembly and appropriate mechanical properties. The process requires: i) the optimization of homogeneous and stable slurries and ii) the selection of effective operative parameters. Reticulation techniques were also employed to modifying the collagen surfaces and to achieve good resistance to physiological environment.
S29
Regenerative medicine has been also shown to be a potential alternative to cardiac transplantation for patients with severe heart failure. In this view a modified tape-casting technique was used to obtain thin preformed collagenous scaffolds/membranes to be tested as a potential vector for the transplantation of cardiomyocytes. Indeed the efficacy of the scaffolds endowed with contracting cardiac cell sheets was evaluated after application onto the injured area of rat infarcted myocardium in order to improve cardiac function. doi:10.1016/j.jbiotec.2010.08.084 [C.8] Drug profiling and biomarker discovery spectrometry-based proteomics technologies
using
mass
A. Jenne ∗ , A. Tebbe, C. Schaab, K. Godl, M. Kaminski, S. Müller KINAXO Biotechnologies GmbH, Germany Keywords: Phosphoproteomics; Chemical proteomics; Biomarker; Targeted drugs System-wide approaches integrating drug target identification and global phosphoproteome analysis provide detailed insights into the cellular mode of action of targeted cancer drugs. Proteome labeling methods such as stable isotope labeling by amino acids in cell culture (SILAC) are employed for quantitative mass spectrometry analysis of cell line and tissue samples. Combining these methods with state-of-the-art chemical proteomics technologies help reveal a compound’s protein affinities and its impact on signal transduction pathways. KINAXO has applied this approach to sorafenib (NexavarTM , Bayer Healthcare), a targeted drug approved for the treatment of kidney and liver cancer. Our findings suggest a previously unpublished mode of action for sorafenib through inhibition of the mTOR pathway in prostate cancer cells (PC-3).
doi:10.1016/j.jbiotec.2010.08.085