- Email: [email protected]
Comment on the article “Investigation of Fluorescence Resonance Energy Transfer between Fluorescein and Rhodamine 6G”
Accepted Manuscript Comment on the article “Investigation of Fluorescence Resonance Energy Transfer between Fluorescein and Rhodamine 6G”
Neeraj Kumar Joshi, Sanjay Pant, Hem Chandra Joshi PII: DOI: Reference:
S1386-1425(16)30677-1 doi: 10.1016/j.saa.2016.11.023 SAA 14783
To appear in:
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
Received date: Accepted date:
23 June 2015 15 November 2016
Please cite this article as: Neeraj Kumar Joshi, Sanjay Pant, Hem Chandra Joshi , Comment on the article “Investigation of Fluorescence Resonance Energy Transfer between Fluorescein and Rhodamine 6G”. The address for the corresponding author was captured as affiliation for all authors. Please check if appropriate. Saa(2016), doi: 10.1016/ j.saa.2016.11.023
This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
ACCEPTED MANUSCRIPT Comment on the article “Investigation of Fluorescence Resonance Energy Transfer between Fluorescein and Rhodamine 6G”
Neeraj Kumar Joshia,#, Sanjay Panta*, Hem Chandra Joshib* Photophysics Laboratory, Department of Physics, DSB Campus, Kumaun University,
IP
Nainital 263002, Uttarakhand, India
T
a
b
CR
Institute for Plasma Research, Laser Diagnostics Division, Bhat, Near Indira Bridge,
AN
US
Gandhinagar 382428, Gujarat, India
M
Abstract
In this comment we, report the missing of relevant literature regarding Forster energy transfer (FRET)
ED
between fluorescein and rhodamine 6G in a recent paper (Spectrochim. Acta A, 149 (2015) 143–149)
PT
.In this paper, the authors claim that “a new FRET pair” has been identified, which is absolutely incorrect. In fact, studies on FRET in this dye pair under different conditions have been done earlier. Further, the
CE
estimated critical transfer distance may have uncertainty because of donor quantum yield which is not
AC
clarified in the paper.
Key Words: FRET; dye pair; quantum yield
*e-mail: [email protected](Sanjay Pant), [email protected] (H. C. Joshi) #
Present Address: Laser Chemistry Group, Department of Chemistry, University of
Saskatchewan, Saskatoon S7N 5C9, Canada
ACCEPTED MANUSCRIPT
In a recent paper [1], Saha et al. report FRET between the dye pair from fluorescein (Flu) and Rhodamine 6G (Rh6G) in aqueous solution in presence as well as in absence of synthetic clay laponite. In the introduction section they mention that “to the best our knowledge, FRET
IP
T
between Flu and Rh6G has never been reported”. In the conclusion section also, they say that “a new FRET pair has been identified”. This is far from actual facts and it appears that the authors
CR
did not do the proper survey of the literature in FRET in this system [2-6]. In fact it’s a known
US
FRET dye pair and has been exploited extensively. Therefore, the conclusion that “a new FRET pair has been identified” is unwarranted.
AN
Moreover, authors have studied the ET efficiency of Flu-R6G pair at different pH ranging
M
from 1.8 to 8. Various reports have been published regarding fluorescence properties e.g
ED
quantum yield and life time of FL depending on the pH [7-10]. Various ionic species will be present in aqueous solution depending on the pH and the quantum yield varies substantially
PT
[7,9,10]. In view of this, measured R0 values (from Eq. 4) at different pH (in table 3) may have
CE
uncertainty as Eq. 4 contains a term of quantum yield of donor (although it has 1/6 power dependence with quantum yield). However, authors do not mention anything about the quantum
AC
yield at different pH values except in aqueous solution (at pH =7) only.
ACCEPTED MANUSCRIPT
References [1]
J. Saha, A. D. Roy, D. Dey, S. Chakraborty, D. Bhattacharjee, P.K. Paul, S. A. Hussain,
T
Spectrochim Acta Part A,149 (2015) 143–149. R. P. S. Kushwaha, D. D. Pant, Indian J. of Pure and Appl. Phys., 23 (1985) 442-446.
[3]
K. K. Pandey, H.C. Joshi and T.C. Pant, Chem. Phys. Lett., 148 (1988) 472 – 478.
[4]
P. Bojarski, L. Kułak, J. Kubicki, Chem. Phys. Lett., 318 (2000) 379–384.
[5]
V. Misra, H. Mishra, J. Chem. Phys.,127 (2007) 094511-10.
[6]
A. Tsuchida, Y. Ohi, S. Takahashi, H. Kimura, T. Okubo, Macromol. Symp., 2010, 288,
AN
US
CR
IP
[2]
M
87–94.
M. Martin, L. Lindquist, J. Lumin., 10 (1975) 381-390.
[8]
E. Martin, A. Pardo, M.S. Guijarro, J.I. Fernandez-Alonso, J. of Mol. Struc., 142 (1986)
ED
[7]
PT
197–200.
R. Sjoback, J. Nygren, M. Kubista, Spectrochim Acta A, 51 (1995) L7-L21.
[10]
S. Pant, H.B. Tripathi, D.D. Pant, J. Photochem. Photobiol. A, 81 (1994) 7-11.
AC
CE
[9]
ACCEPTED MANUSCRIPT
AC
CE
PT
ED
M
AN
US
CR
IP
T
Graphical Abstract
Neeraj Kumar Joshi, Sanjay Pant, Hem Chandra Joshi PII: DOI: Reference:
S1386-1425(16)30677-1 doi: 10.1016/j.saa.2016.11.023 SAA 14783
To appear in:
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
Received date: Accepted date:
23 June 2015 15 November 2016
Please cite this article as: Neeraj Kumar Joshi, Sanjay Pant, Hem Chandra Joshi , Comment on the article “Investigation of Fluorescence Resonance Energy Transfer between Fluorescein and Rhodamine 6G”. The address for the corresponding author was captured as affiliation for all authors. Please check if appropriate. Saa(2016), doi: 10.1016/ j.saa.2016.11.023
This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
ACCEPTED MANUSCRIPT Comment on the article “Investigation of Fluorescence Resonance Energy Transfer between Fluorescein and Rhodamine 6G”
Neeraj Kumar Joshia,#, Sanjay Panta*, Hem Chandra Joshib* Photophysics Laboratory, Department of Physics, DSB Campus, Kumaun University,
IP
Nainital 263002, Uttarakhand, India
T
a
b
CR
Institute for Plasma Research, Laser Diagnostics Division, Bhat, Near Indira Bridge,
AN
US
Gandhinagar 382428, Gujarat, India
M
Abstract
In this comment we, report the missing of relevant literature regarding Forster energy transfer (FRET)
ED
between fluorescein and rhodamine 6G in a recent paper (Spectrochim. Acta A, 149 (2015) 143–149)
PT
.In this paper, the authors claim that “a new FRET pair” has been identified, which is absolutely incorrect. In fact, studies on FRET in this dye pair under different conditions have been done earlier. Further, the
CE
estimated critical transfer distance may have uncertainty because of donor quantum yield which is not
AC
clarified in the paper.
Key Words: FRET; dye pair; quantum yield
*e-mail: [email protected](Sanjay Pant), [email protected] (H. C. Joshi) #
Present Address: Laser Chemistry Group, Department of Chemistry, University of
Saskatchewan, Saskatoon S7N 5C9, Canada
ACCEPTED MANUSCRIPT
In a recent paper [1], Saha et al. report FRET between the dye pair from fluorescein (Flu) and Rhodamine 6G (Rh6G) in aqueous solution in presence as well as in absence of synthetic clay laponite. In the introduction section they mention that “to the best our knowledge, FRET
IP
T
between Flu and Rh6G has never been reported”. In the conclusion section also, they say that “a new FRET pair has been identified”. This is far from actual facts and it appears that the authors
CR
did not do the proper survey of the literature in FRET in this system [2-6]. In fact it’s a known
US
FRET dye pair and has been exploited extensively. Therefore, the conclusion that “a new FRET pair has been identified” is unwarranted.
AN
Moreover, authors have studied the ET efficiency of Flu-R6G pair at different pH ranging
M
from 1.8 to 8. Various reports have been published regarding fluorescence properties e.g
ED
quantum yield and life time of FL depending on the pH [7-10]. Various ionic species will be present in aqueous solution depending on the pH and the quantum yield varies substantially
PT
[7,9,10]. In view of this, measured R0 values (from Eq. 4) at different pH (in table 3) may have
CE
uncertainty as Eq. 4 contains a term of quantum yield of donor (although it has 1/6 power dependence with quantum yield). However, authors do not mention anything about the quantum
AC
yield at different pH values except in aqueous solution (at pH =7) only.
ACCEPTED MANUSCRIPT
References [1]
J. Saha, A. D. Roy, D. Dey, S. Chakraborty, D. Bhattacharjee, P.K. Paul, S. A. Hussain,
T
Spectrochim Acta Part A,149 (2015) 143–149. R. P. S. Kushwaha, D. D. Pant, Indian J. of Pure and Appl. Phys., 23 (1985) 442-446.
[3]
K. K. Pandey, H.C. Joshi and T.C. Pant, Chem. Phys. Lett., 148 (1988) 472 – 478.
[4]
P. Bojarski, L. Kułak, J. Kubicki, Chem. Phys. Lett., 318 (2000) 379–384.
[5]
V. Misra, H. Mishra, J. Chem. Phys.,127 (2007) 094511-10.
[6]
A. Tsuchida, Y. Ohi, S. Takahashi, H. Kimura, T. Okubo, Macromol. Symp., 2010, 288,
AN
US
CR
IP
[2]
M
87–94.
M. Martin, L. Lindquist, J. Lumin., 10 (1975) 381-390.
[8]
E. Martin, A. Pardo, M.S. Guijarro, J.I. Fernandez-Alonso, J. of Mol. Struc., 142 (1986)
ED
[7]
PT
197–200.
R. Sjoback, J. Nygren, M. Kubista, Spectrochim Acta A, 51 (1995) L7-L21.
[10]
S. Pant, H.B. Tripathi, D.D. Pant, J. Photochem. Photobiol. A, 81 (1994) 7-11.
AC
CE
[9]
ACCEPTED MANUSCRIPT
AC
CE
PT
ED
M
AN
US
CR
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Graphical Abstract