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COMPARATIVE STUDY AMONG HISTOLOGIC GRADUATION SYSTEMS OF ORAL EPITHELIAL DYSPLASIAS
ABSTRACTS
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IMMUNOHISTOCHEMICAL COMPARATIVE STUDY OF PERIPHERAL, PERI-IMPLANT, AND CENTRAL GIANT CELL LESIONS. FABIO RAMOA PIRES, THAYNA MELO DE LIMA MORAIS, CIRO DANTAS SOARES, JOSE MANUEL AGUIRRE URIZAR, JAVIER ALBERDI NAVARRO and, OSLEI PAES DE ALMEIDA Central giant cell lesions (CGCL) and peripheral giant cell lesions (PGCL) are reactive lesions of unknown etiopathogenesis and similar histologic features. PGCL affects the gingiva and alveolar mucosa, but some rare cases can be associated with dental implants. Objective: In the present study, we compared the expression of smooth muscle actin, Bcl-2, GLUT-1, CD68, OPG, RANK, Ki67, and CD34 in a series of CGCL and conventional and periimplant PGCL. Study Design: Clinical data were retrieved from the laboratory records and histologic analysis was performed in hematoxylin and eosin (H&E)-stained sections. Immunohistochemical reactions for detecting the above mentioned antibodies were performed and digitally scored. Results: The digital scores for CD68 and Bcl-2 were higher in conventional PGCL and CGCL than in peri-implant PGCL (P = .033 for CD68 and P <.0001 for Bcl-2). Microvessel density was higher in the conventional PGCL than in CGCL and periimplant PGCL (P = .002). Conclusions: The current results demonstrated some differences in microvessel density, proliferative activity, and expression of CD68 and Bcl-2 among conventional PGCL, periimplant PGCL and CGCL. Further studies are encouraged to better understanding of these early findings.
COMPARATIVE STUDY AMONG HISTOLOGIC GRADUATION SYSTEMS OF ORAL EPITHELIAL DYSPLASIAS. JULIANA COSTA DE OLIVEIRA, GABRIELA MEDEIROS DE CERQUEIRA, e JOAO FIGUEIRA SCARINI, PRISCILLA RODRIGUES ^ CAMARA, REBECA DE SOUZA AZEVEDO, DECIO DOS SANTOS PINTO JUNIOR and, RENATA TUCCI Objective: The objective of this study was to evaluate the histologic grading systems for oral epithelial dysplasia (ODE). Study Design: One hundred slides of ODE containing cases selected from 2 distinct oral pathology services were examined by 4 evaluators, who classified each case according to the architectural and cytologic criteria and were diagnosed according to the World Health Organization (WHO) system and binary. In addition to these 2 systems, a third form of analysis was considered, taking into account the presence or absence of ODE. Results: The results were statistically analyzed in a descriptive way and by the correlation methods (Kendall tau-B and Phi test) and concordance (kappa). The WHO graduation system presented lower agreement among the evaluators; the best agreement among the evaluators occurred when considering the presence or absence of dysplasia; and in addition, it has been observed that less experienced oral pathologists tend to have more severe diagnoses than those with a longer career.
OOOO January 2020 Conclusions: The lack of interobserver agreement was evident, highlighting the need for further studies, due to the great importance and relevance of OEDs.
EOSINOPHILIC ULCER OF THE ORAL MUCOSA: A CLINICOPATHOLOGIC STUDY OF 30 CASES. MARIA FERNANDA CALAZANS FERNANDES, BEATRIZ CORDEIRO, ELLEN BRILHANTE DE ALBUQUERQUE CORTEZZI, ROMAN CARLOS, BRUNO AUGUSTO BENEVENUTO DE ANDRADE, MICHELLE AGOSTINI and, MARIO JOSE e ROMANACH Objective: The aim of this study was to describe the clinicopathologic features of 30 new cases of oral eosinophilic ulcers. Study Design: Clinical data concerning sex, age, affected site, and clinical presentation of 30 patients from Latin America were collected from the files of 2 Oral Pathology Laboratories in a period between 1982 and 2018. Results: The mean age of the patients was 53.3 years, with slight predilection for female patients (1.2:1). Medical history of one-third of patients included psychiatric disorders, alcohol drinking, diabetes mellitus, and human immunodeficiency virus (HIV) infection. The tongue was the most common affected site (76.7%) by lesions with mean size of 1.8 cm and mean evolution of 5.4 months. Raised edges, pain and clinical suspicion of squamous cell carcinoma (SCC) were reported in almost half of cases. Microscopically, all cases revealed a connective tissue covered by fibrinopurulent membrane, containing many capillaries with prominent endothelial cells and reactive inflammatory infiltrate. Variable number of eosinophils was observed, mainly located in the lamina propria, but also deeply between muscle fibers. Conclusions: Eosinophilic ulcer is likely a reactive painful lesion that mainly affects the tongue of adults, which may clinically mimic oral SCC.
PREVALENCE OF ORAL LESIONS IN INDIVIDUALS UP TO 19 YEARS OF AGE: ANALYSIS OF BIOPSIED CASES FROM A SINGLE e VICTOR SILVA BETT, GILBERTO INSTITUTION. JOAO ^ DE SOUZA MELO, ELIS ANGELA BATISTELLA and, ELENA RIET CORREA RIVERO Objective: To determine and compare the prevalence of oral lesion in patients aging from 0 to 9 years and 10 to 19 years. Study Design: Records of biopsies from a single-institution laboratory of oral pathology were screened and data regarding sex, age, and diagnosis were collected. Pearson chi-squared test was applied to determine significant differences on prevalence of lesions between the 2 groups. Results: At total, 296 lesions were assessed; 84 cases from individuals younger than 9 years old and 212 from individuals from 10 to 19 years of age. Overall, mucocele and pyogenic granuloma were the most common lesions diagnosed. A significantly higher prevalence of intraosseous lesions, odontogenic cysts, reactive lesions, and salivary gland lesions were found in individuals aging from 10 to 19 years compared to the younger group (P .05). Conclusions: Mucocele and pyogenic granuloma were the most frequently observed lesions in this study. Individuals aging from 10 to 19 years of age presented significantly higher prevalence
e178
IMMUNOHISTOCHEMICAL COMPARATIVE STUDY OF PERIPHERAL, PERI-IMPLANT, AND CENTRAL GIANT CELL LESIONS. FABIO RAMOA PIRES, THAYNA MELO DE LIMA MORAIS, CIRO DANTAS SOARES, JOSE MANUEL AGUIRRE URIZAR, JAVIER ALBERDI NAVARRO and, OSLEI PAES DE ALMEIDA Central giant cell lesions (CGCL) and peripheral giant cell lesions (PGCL) are reactive lesions of unknown etiopathogenesis and similar histologic features. PGCL affects the gingiva and alveolar mucosa, but some rare cases can be associated with dental implants. Objective: In the present study, we compared the expression of smooth muscle actin, Bcl-2, GLUT-1, CD68, OPG, RANK, Ki67, and CD34 in a series of CGCL and conventional and periimplant PGCL. Study Design: Clinical data were retrieved from the laboratory records and histologic analysis was performed in hematoxylin and eosin (H&E)-stained sections. Immunohistochemical reactions for detecting the above mentioned antibodies were performed and digitally scored. Results: The digital scores for CD68 and Bcl-2 were higher in conventional PGCL and CGCL than in peri-implant PGCL (P = .033 for CD68 and P <.0001 for Bcl-2). Microvessel density was higher in the conventional PGCL than in CGCL and periimplant PGCL (P = .002). Conclusions: The current results demonstrated some differences in microvessel density, proliferative activity, and expression of CD68 and Bcl-2 among conventional PGCL, periimplant PGCL and CGCL. Further studies are encouraged to better understanding of these early findings.
COMPARATIVE STUDY AMONG HISTOLOGIC GRADUATION SYSTEMS OF ORAL EPITHELIAL DYSPLASIAS. JULIANA COSTA DE OLIVEIRA, GABRIELA MEDEIROS DE CERQUEIRA, e JOAO FIGUEIRA SCARINI, PRISCILLA RODRIGUES ^ CAMARA, REBECA DE SOUZA AZEVEDO, DECIO DOS SANTOS PINTO JUNIOR and, RENATA TUCCI Objective: The objective of this study was to evaluate the histologic grading systems for oral epithelial dysplasia (ODE). Study Design: One hundred slides of ODE containing cases selected from 2 distinct oral pathology services were examined by 4 evaluators, who classified each case according to the architectural and cytologic criteria and were diagnosed according to the World Health Organization (WHO) system and binary. In addition to these 2 systems, a third form of analysis was considered, taking into account the presence or absence of ODE. Results: The results were statistically analyzed in a descriptive way and by the correlation methods (Kendall tau-B and Phi test) and concordance (kappa). The WHO graduation system presented lower agreement among the evaluators; the best agreement among the evaluators occurred when considering the presence or absence of dysplasia; and in addition, it has been observed that less experienced oral pathologists tend to have more severe diagnoses than those with a longer career.
OOOO January 2020 Conclusions: The lack of interobserver agreement was evident, highlighting the need for further studies, due to the great importance and relevance of OEDs.
EOSINOPHILIC ULCER OF THE ORAL MUCOSA: A CLINICOPATHOLOGIC STUDY OF 30 CASES. MARIA FERNANDA CALAZANS FERNANDES, BEATRIZ CORDEIRO, ELLEN BRILHANTE DE ALBUQUERQUE CORTEZZI, ROMAN CARLOS, BRUNO AUGUSTO BENEVENUTO DE ANDRADE, MICHELLE AGOSTINI and, MARIO JOSE e ROMANACH Objective: The aim of this study was to describe the clinicopathologic features of 30 new cases of oral eosinophilic ulcers. Study Design: Clinical data concerning sex, age, affected site, and clinical presentation of 30 patients from Latin America were collected from the files of 2 Oral Pathology Laboratories in a period between 1982 and 2018. Results: The mean age of the patients was 53.3 years, with slight predilection for female patients (1.2:1). Medical history of one-third of patients included psychiatric disorders, alcohol drinking, diabetes mellitus, and human immunodeficiency virus (HIV) infection. The tongue was the most common affected site (76.7%) by lesions with mean size of 1.8 cm and mean evolution of 5.4 months. Raised edges, pain and clinical suspicion of squamous cell carcinoma (SCC) were reported in almost half of cases. Microscopically, all cases revealed a connective tissue covered by fibrinopurulent membrane, containing many capillaries with prominent endothelial cells and reactive inflammatory infiltrate. Variable number of eosinophils was observed, mainly located in the lamina propria, but also deeply between muscle fibers. Conclusions: Eosinophilic ulcer is likely a reactive painful lesion that mainly affects the tongue of adults, which may clinically mimic oral SCC.
PREVALENCE OF ORAL LESIONS IN INDIVIDUALS UP TO 19 YEARS OF AGE: ANALYSIS OF BIOPSIED CASES FROM A SINGLE e VICTOR SILVA BETT, GILBERTO INSTITUTION. JOAO ^ DE SOUZA MELO, ELIS ANGELA BATISTELLA and, ELENA RIET CORREA RIVERO Objective: To determine and compare the prevalence of oral lesion in patients aging from 0 to 9 years and 10 to 19 years. Study Design: Records of biopsies from a single-institution laboratory of oral pathology were screened and data regarding sex, age, and diagnosis were collected. Pearson chi-squared test was applied to determine significant differences on prevalence of lesions between the 2 groups. Results: At total, 296 lesions were assessed; 84 cases from individuals younger than 9 years old and 212 from individuals from 10 to 19 years of age. Overall, mucocele and pyogenic granuloma were the most common lesions diagnosed. A significantly higher prevalence of intraosseous lesions, odontogenic cysts, reactive lesions, and salivary gland lesions were found in individuals aging from 10 to 19 years compared to the younger group (P .05). Conclusions: Mucocele and pyogenic granuloma were the most frequently observed lesions in this study. Individuals aging from 10 to 19 years of age presented significantly higher prevalence