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Abstracts / Atherosclerosis 235 (2014) e192–e301
A. Pereira, F.O.C. Pereira, T.C. Pereira, M.C. Elias, S.S.M. Ihara, C.A. Machado, R.D. Santos Filho, T.L.R. Martinez
assessed (10-9 – 3x10-5 mol/L). 14 mM sections from aortic arch and SMA were stained with dihydroethidium and in situ superoxide anion production was measured by confocal microscopy. Results: There was significant elevation in serum lipids and nitrites of HF fed animals. 1% and 5% RBEE supplementation reduced such increase (p<0.05 and p<0.01, respectively) while total cholesterol decreased only in HF 5% group (p<0.05). HDL cholesterol and HDL/total cholesterol ratio augmented in HF 1% and HF 5% (p<0.05 and p<0.001, respectively). Endothelial function assessed by ACh-induced dilatation was impaired in HF while partially maintained in ST group. Supplement of 1% or 5% RBEE restored such impairment in HF groups (p<0.001). Experiments in the presence of NOS inhibitor L-NAME revealed that NO participation was enhanced in HF1% and 5% whereas EDHF remained unaffected. HF increased vascular superoxide production in SMA and aorta, being 1% RBBE able to reduce such superoxide release. RBEE supplementation also reduced superoxide production in aorta from ST treated groups (p<0.01). Conclusion: RBEE supplemented diet improved lipid profile, restored endothelium dependant dilatation and decreased superoxide production showing its interest as functional food in atherosclerosis disease.
Cardiology, HCFMUSP, Sao Paulo, Brazil
65 - Dietary and bariatric interventions
Objectives: The process of nutritional transition that occurred in the twentieth century , led to changes in dietary habits increasing CVD Risk.
EAS-0994. EVALUATION OF DIETARY HABITS AND THE NUTRITIONAL CONTENT OF THE INTAKE FOOD TO OVERWEIGHT AND OBESE PATIENTS
Miniaturas de anexos Vídeos detectados neste e-mail Baixar todos os anexos Todos os anexos Só multimídias Só documentos Só executáveis Outros Todas as mídias embutidas neste email Exibir todas as fotos (slideshow) Atenção: anexos podem danificar e expor seu computador a riscos. Saiba mais. Conclusion: The correlation with food consumption above recommended and inappropriate percentiles of waist circumference , reveals population risk factor for cardiovascular disease . the future of our country . 65 - Dietary and bariatric interventions EAS-1010. COMPARISON BETWEEN TOTAL DIETARY INTAKE AND SERUM CHOLESTEROL LEVELS IN ELEMENTARY AND MIDDLE SCHOOL STUDENTS
Based on this fact , we sought to identify an association between dietary intake and serum cholesterol level in public school students. Methods: A prospective cross sectional study of 276 students of both genders , aged 6-13 years who were divided into 2 groups according to the extent of cholesterol , group 1 < 170 mg / dL (n ¼ 186)and 2 170 mg / dL (n ¼ 86) . We assessed dietary intake record by 24 hours Nutwin software program . TCholesterol was obtained by finger tip sample assay. Statistics: Student's t test in normal distribution and Mann Whitney in non normal . Results: The results , although in accordance with the recommended daily intakes (RDI, 2002) , showed increased protein intake (18.64 6.37 %) and lower carbohydrate (52.46 7.34 %) in group 1 (p ¼ 0.015 and 0.025 respectively) , suggesting increased consumption of animal protein with high levels of saturated fat . The other variables analyzed results with no statistical significance was observed , although reported to be inadequate fiber intake in both groups . (group 1 ¼ 19.71 9.17 g and group 2 ¼ 19.81 7.82 g) . Conclusion: We found a relationship between higher intakes of protein and less carbohydrates with high levels of serum cholesterol. Based on these results we can conclude the importance of intervention starting early in life. 65 - Dietary and bariatric interventions EAS-0307. RICE BRAN ENZYMATIC EXTRACT REDUCES OXIDATIVE STRESS AND RESTORES MESENTERIC ENDOTHELIUM DEPENDANT DILATATION IN APO E (-/-) MICE C. Perez Ternero, C. Claro, R. Rodriguez-Rodriguez, M. Alvarez de Sotomayor, M.D. Herrera Department of Pharmacology School of Pharmacy, University of Seville, Seville, Spain Objectives: Rice bran is a byproduct of rice milling rich in antioxidants, sterols and g-oryzanol that is underused mainly due to its low water solubility. A novel enzymatic extraction method gives rise to 5 fold goryzanol content and improves water solubility. We aimed to evaluate the effect of a rice bran enzymatic extract (RBEE) supplemented diet on atherosclerosis. Methods: Seven week-old male apolipoprotein E knockout mice were fed standard (ST) or high fat (HF) diet supplemented or not with 1% or 5% RBEE for 23 weeks. Serum lipids and nitrites were measured using spectrophotometry and Griess reaction, respectively. Small mesenteric arteries (SMA) were mounted in a pressure myograph and ACh response was
M. Poseaa, A. Dragomirb, E. Rusua, R. Nana, R. Draguta, H. Popescua, F. Stoicescua, I. Teodoruc, A. Hancub, M. Stoiand, G. Raduliana a Diabetes II, National Institute of Diabetes Nutrition and Metabolic Diseases, Bucharest, Romania; b Diabetes, University of Medicine and Pharmacy "Carol Davila", Bucharest, Romania; c Dialysis, National Institute of Diabetes Nutrition and Metabolic Diseases, Bucharest, Romania; d Internal Medicine, Clinical Hospital "Dr. I Cantacuzino", Bucharest, Romania
Objectives: To document the nutritional content of food intake in overweight and obese people, in order to change dietary habits for acquiring the normal weight Methods: To a sample of 124 overweight and obese people and another one of 33 patients with normal weight we assessed the nutritional contents of the intake food: calories, vitamins and minerals among these patients. We also measured abdominal circumference, percent of body fat and resting metabolic rate Results: Overweight and obese patients, compare to normalweight people, have a significant increased intake of vitamin A (p¼0.002), vitamin B1 (p¼0.037), vitamin B5 (p¼0.047), vitamin C (p¼0.029) and vitamin D (p¼0.015) and eat more sodium (p¼0.036), iron (p¼0.032) and selenium (p¼0.007). The normalweight patients eat more than 2 g/day sodium. The percent of body fat in obese persons is increased (p¼0.00001) and very close to abnormal in patients with no weight problems. An increased food intake, above resting metabolic rate calculated is associated with a slower metabolism and a rapid resting metabolic rate is often associated with food intake below resting metabolic rate (p¼0.002). Conclusion: All the patients had imbalanced intake of vitamins and minerals. The normal weight group didn't achieve the dietary recommendations for vitamins and minerals and we found that this patients had risk factors for cardiac diseases like increased abdominal circumference, body fat and sodium intake. 65 - Dietary and bariatric interventions EAS-1103. FUNCTIONAL OLIVE OILS FUNCTIONS R. Solaa, R.M. Vallsb, M. Farrasc, M. Suarezd, S. Fernandez-Castillejoe, M. Fitof, V. Konstantinidoue, A. Pedrete, F. Fuentesg, J. Lopez-Mirandag, M. Giralth, M.I. Covasc, M.J. Motilvad a Internal Medicine, Facultat de Medicina i Ciències de la Salut. Universitat Rovira i Virgili., Reus, Spain; b Research Unit on Lipids and Atherosclerosis,