- Email: [email protected]
Comparison differentiated osteoblast from adipose stem cells and normal osteoblast
Abstracts
Interventional Cardiology, School of medicine, Mashhad University of Medical Sciences, Mashhad, Iran E-mail address: [email protected] (M.S. Mahmoud)
S65
Keywords: Dilated cardiomyopathy, Apoptosis, Heart failure, tachycardia doi:10.1016/j.clinbiochem.2011.08.134
Introduction: We aimed on seeking coronary artery involvement in mustard gas-intoxicated patients. Methods: This is a case-control study conducted on 40 mustard gasintoxicated patients whom had undergone coronary artery angiography due to cardiac pain. It was performed during a 3-year period from 2008 to 2010 on patients referring to Qaem, Imam Reza and Razavi hospitals, Mashad, Iran. The control group consisted of 40 normal individuals whom had undergone angiography for the same reasons. The primary outcome measure was coronary artery involvement and its location. Required data was collected through studying the angiography films. Results: Among the 40 studied intoxicated patients, 15 (37.5%) had coronary artery ectasia, mainly in the Left Anterior Descending artery while 25 (62.5%) did not. The same values were 2 (5%) and 38 (95%) in the control group, respectively which showed a significant difference when compared to the study group (p = 0.001). The odds ratio was 11.40. Conclusions: The prevalence of coronary artery ectasia in the mustard-intoxicated patients was 7.5 times more than the controls. Considering the proposed odds ratio, coronary artery ectasia occurs around 11.4 times more in mustard gas-intoxicated veterans. This is the first study suggesting a strong correlation between mustard gas intoxication and coronary artery ectasia. Keywords: Coronary artery ectasia, Aneurysm, Mustard gas doi:10.1016/j.clinbiochem.2011.08.133
Poster – [A-10-1348-1] Involvement of cardiomyocytes apoptosis in a canine model of chronic heart failure induced by tachycardia M. Mahmoudabadya, R. Naeijeb, K. McEnteeb a Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran b Laboratory of Physiology, Université Libre de Bruxelles, Brussels, Belgium E-mail address: [email protected] (M. Mahmoudabady) Introduction: Apoptosis has been observed in cardiomyopathic conditions. However, the participation of apoptosis during the development of pacing- induced dilated cardiomyopathy is not fully understood. Methods: In our model of 7 weeks rapid right ventricular pacing , gene expressions of Bax, Bcl-2 and Caspase-3 were measured by RTQPCR from interventricular septum biopsies that were taken weekly in 21 beagle dogs during the development of heart failure. We evaluated protein levels of these genes by Western Blot and DNA fragmentation by TUNEL method from autopsy samples. 8 normal beagle dogs were used as control group. Results: Gene expression of Bax remained unchanged during the pacing period, Bcl-2 mRNA expression transiently decreased in moderate heart failure and their ratio (Bcl-2/Bax) was not significantly altered. Caspase-3 gene expression increased in heart failure. Compared to the control group, expression of Bax and Bcl-2 proteins and their ratio were increased in dogs after 4 weeks of pacing, but not after 7 weeks of pacing. No band of activated caspase was found in the normal nor in the paced myocardium. In the TUNEL assay there was no significant difference between numbers of apoptotic cells in all groups, although a few TUNEL-positive cells were detected in the dogs paced for 4 and 7 weeks. Conclusion: Altogether and in spite of previous studies our results are not in favour of apoptosis as a major mechanism in the pathogenesis of heart failure.
E Poster – [A-10-1352-1] High prevalence of metabolic syndrome in Iran in comparison to France What are the explanatory components? M. Azimi-Nezhada, Herbetha, G. Siesta, H. Esmailib, S.J. Hosseinic, M. Ghayour-Mobarhanc, S. Visvikis-Siestaa a Unité de recherche “Génétique Cardiovasculaire”, EA-4373, Université Henri Poincaré-Nancy 1, Faculté de Pharmacie, 30, rue Lionnois, 54000 Nancy, France b Department of Biostatistics and Epidemiology, Faculty of Health, Mashhad University of Medical Sciences, 9138813944 Mashhad, Iran c Biochemistry and Nutrition Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, 9138813944 Mashhad, Iran E-mail address: [email protected] (M. Azimi-Nezhad) Introduction: Several studies have shown a high prevalence of metabolic syndrome (MetS) and low HDL-C levels in Iranian populations. The aim of this study was to investigate the differences in the prevalence of MetS and of its components between an Iranian and a French population and give hypotheses for the origin of these differences. Methods and results: Prevalence of MetS and related abnormalities was estimated according to ATPIII definition in 1386 adults from France and 1194 adults from Iran of both sexes. MetS prevalence was the highest in Iranian women (55.0%), followed by Iranian men (30.1%), French men (13.7%) and French women (6.6%); all the prevalences were significantly different from each other. Iranian women were characterized by high rates of abdominal obesity (65.0%), hypertension (52.1%), hypertriglyceridemia (43.1%) and low HDL-cholesterol (92.7%). Iranian men were characterized by high rates of hypertension (48.9%), hypertriglyceridemia (42.8%) and low HDL-cholesterol (81.8%). French men had high rates of hypertension (44.7%) and mild rates of hypertriglyceridemia (28.6%) and hyperglycemia (23.9%). Pearson correlation coefficients between waist circumference and other MetS components were generally stronger in French individuals in comparison to Iranian, whatever the sex, for systolic blood pressure, HDL-cholesterol and fasting glucose and in French men only for triglycerides and diastolic blood pressure. Conclusion: The main finding of this study is a high prevalence of low HDL-C concentration in the Iranian population especially in Iranian women compared to French women. Other differences in metabolic syndrome criteria between the two countries may be mainly due to the environmental differences including life style, physical activity, dietary pattern and probably genetic factors of the two populations. Keywords: Metabolic syndrome prevalence, Metabolic syndrome component, Waist, Blood pressure, HDL cholesterol, Triglyceride, Glucose, Age, Country differences, Iran, France doi:10.1016/j.clinbiochem.2011.08.135
Histochemistry E Poster – [A-10-85-1] Comparison differentiated osteoblast from adipose stem cells and normal osteoblast Jafary Farzanehab, Esmaeili Nafiseha, Sadeghi Farzaneha, Jafary Faribaa a Department of Biology, Faculty of Basic Sciences, Alzahra University, Tehran, Iran
S66
Abstracts
b
Department of Anatomical Sciences, School of Medicine, Isfahan University of Medicine Sciences, Isfahan, Iran E-mail address: [email protected] (J. Farzaneh) Introduction: Bone is a strange tissue playing critical role in vital organs, blood production, mineral storage and homeostasis, blood PH setting and others. Bone tissue consists of mineralized components [such as calcium-phosphate mineral called hydroxyapatite], marrow, nerves, blood vessels, cartilage and three types of cells: osteoblast, osteocytes and osteoclasts. Osteoblasts are cells that have differentiated from mesenchymal stem cells and important roles in produce and secretes organic and inorganic bone matrix. Therefore osteoblast is a requisite cell in produced extra cellular matrix and regeneration of bone in many diseases such osteoporosis, osteomyelitis, osteoarthritis. Thus differentiation of osteoblast from stem cell is the main subject in tissue engineering. Method: In this study mesenchymal stem cells were isolated from adipose tissues by enzymatic digestive and were differentiated to osteoblast using osteogenic medium then these cells were caparisoned in protein levels, mineral matrix and morphology with normal osteoblasts were isolated from calvarias. Result: This result indicated that protein and mineral material production were not significant different in normal and differentiated osteoblast cells. Conclusion: Therefore according to the result we can use differentiated osteoblast from stem cell instead of bone transplant or nature osteoblast in bone defects. Keywords: Stem cell, Osteoblast, Cell differentiated doi:10.1016/j.clinbiochem.2011.08.136
E Poster – [A-10-782-1] Distribution pattern of some glycoconjugates of hair follicle regeneration in rat Saeed Modaghegh Samane Razavi Hospital, Mashhad, Iran E-mail address: [email protected] Introduction: Regeneration of skin wounds that cause alopsia is one of human problems after accidents or diseases. So introduction of effective drugs or procedures for hair follicle regeneration is important. Tissue regeneration occurs due to cell to cell interactions via the presence of macromolecules such as glycoconjugates that act as cellular receptors in biological events. The aim of present research was identification of some glycoconjugates of regenerating hair follicle. Methods: Ten Sprague rats were anesthetized with pentobarbital intraperitoneally and skin wounds of 4 mm were induced on each rat. These tissues were used as nature intact samples. Repairing wounds were sampled each 5 days until 50th day of post surgery. Paraffin sections were stained with PAS and Alcian blue pH 1, pH 2.5. In microscopic observation color intensity of staining was reported in tables (according to 0–5 gradient). Result: Pas and Alcian blue positive responses were observable (weak intensity) from 15th day in fibrous root sheath and dermal papilla of follicles. High color intensity was seen gradually until 40th day. Conclusion: In this research neutral, sulfated and carboxylated acidic glycoproteins appeared increasingly from mid stage of repairing in some layers of hair follicle. This specific stage distribution is probably related to different cellular activities that improve healing processes. Understanding of molecular aspects of tissue regeneration may be used in pharmacology or tissue engineering. Keywords: Hair follicle, Regeneration, Glycoconjugates
E Poster – [A-10-765-1] The effects of sodium metasilicate on the spinal motoneuron recovery following sciatic nerve injury Nikravesh Mohammad Reza Mashhad University of Medical Sciences, Iran E-mail address: [email protected] Introduction: The anti-oxidant effects of sodium metasilicate (SM) inhibit many oxidative enzymes in liver and kidney. The goal of this research was to study the protective effects of SM on the central degeneration of alpha motoneuron (AM) in the ventral horn of lumber spinal cord of rats which underwent compressive sciatic nerve lesion. Material and methods: The lesioned rats were divided into control, sham and experimental groups and the experimental group treated by 6 mg/kg.ip SM. The rats were kept for a month and then were sacrificed and perfused cardially by 10% formaldehyde and the lumber segments of their spinal cord dissected and sampled. After histological processing, by using a stereological technique, the dissector, the numerical density (Nv) of AM in the ventral horn of lumber spinal cord was estimated in all groups. Results and conclusion: The results showed that although the Nv of AM of sham group is significantly lower than the control (p < 0.001), the Nv of AM of experimental group is higher than shams (p < 0.05). Moreover there is no significant difference between experimental group and control. These results indicate that administration of SM has a beneficial effect on the survival of AM and may prevent or delay the central degeneration. Keywords: Sodium metasilicate, Spinal motoneurons, Sciatic nerve
doi:10.1016/j.clinbiochem.2011.08.137
doi:10.1016/j.clinbiochem.2011.08.138
E Poster – [A-10-785-1] The comparison of the mesenchymal progenitor cell coating state on the MBA and DBM as the biochemical scaffolds Hashemi Zahra Sadat, Forouzandeh Mahdi, Soleimani Masoud Medical Sciences,Tarbiat Modares University, Tehran, Iran E-mail addresses: [email protected] (H.Z. Sadat), [email protected] (F. Mahdi), [email protected] (S. Masoud) Introduction: 3D cultures in the tissue engineering field are a rapidly growing research by using biochemical material as scaffold. These biochemical materials are biocompatible to improve efficiency in the culture. These are used as a delivery vehicle. The most commonly used scaffolds in the bone marrow and cartilage are MBA and DBM. These are based on the bone matrix but the only difference between them is the lack of mineralized materials in DBM by the acid extraction method. Our goal is to understand how important is the presence of the mineral materials for the coating of the cells. Materials and methods: Human placenta mesenchymal progenitor cells were isolated and characterized. For 8 weeks, these cells were cultured in the specific media. Consequently the two scaffolds were immersed in the media culture for 2 hours. After that, cells were added. This suspension was rotated for 30 minutes during the incubation. After 3 days, the media was changed and the granules of two MPC-coating scaffolds were sent for Scanning Electron Microscope. Results: SEM images show the better coating on the demineralized bone matrix (DBM). We had nearly 50% coating cells on the DBM but 30% for MBA (mineralized bone allograft).
Interventional Cardiology, School of medicine, Mashhad University of Medical Sciences, Mashhad, Iran E-mail address: [email protected] (M.S. Mahmoud)
S65
Keywords: Dilated cardiomyopathy, Apoptosis, Heart failure, tachycardia doi:10.1016/j.clinbiochem.2011.08.134
Introduction: We aimed on seeking coronary artery involvement in mustard gas-intoxicated patients. Methods: This is a case-control study conducted on 40 mustard gasintoxicated patients whom had undergone coronary artery angiography due to cardiac pain. It was performed during a 3-year period from 2008 to 2010 on patients referring to Qaem, Imam Reza and Razavi hospitals, Mashad, Iran. The control group consisted of 40 normal individuals whom had undergone angiography for the same reasons. The primary outcome measure was coronary artery involvement and its location. Required data was collected through studying the angiography films. Results: Among the 40 studied intoxicated patients, 15 (37.5%) had coronary artery ectasia, mainly in the Left Anterior Descending artery while 25 (62.5%) did not. The same values were 2 (5%) and 38 (95%) in the control group, respectively which showed a significant difference when compared to the study group (p = 0.001). The odds ratio was 11.40. Conclusions: The prevalence of coronary artery ectasia in the mustard-intoxicated patients was 7.5 times more than the controls. Considering the proposed odds ratio, coronary artery ectasia occurs around 11.4 times more in mustard gas-intoxicated veterans. This is the first study suggesting a strong correlation between mustard gas intoxication and coronary artery ectasia. Keywords: Coronary artery ectasia, Aneurysm, Mustard gas doi:10.1016/j.clinbiochem.2011.08.133
Poster – [A-10-1348-1] Involvement of cardiomyocytes apoptosis in a canine model of chronic heart failure induced by tachycardia M. Mahmoudabadya, R. Naeijeb, K. McEnteeb a Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran b Laboratory of Physiology, Université Libre de Bruxelles, Brussels, Belgium E-mail address: [email protected] (M. Mahmoudabady) Introduction: Apoptosis has been observed in cardiomyopathic conditions. However, the participation of apoptosis during the development of pacing- induced dilated cardiomyopathy is not fully understood. Methods: In our model of 7 weeks rapid right ventricular pacing , gene expressions of Bax, Bcl-2 and Caspase-3 were measured by RTQPCR from interventricular septum biopsies that were taken weekly in 21 beagle dogs during the development of heart failure. We evaluated protein levels of these genes by Western Blot and DNA fragmentation by TUNEL method from autopsy samples. 8 normal beagle dogs were used as control group. Results: Gene expression of Bax remained unchanged during the pacing period, Bcl-2 mRNA expression transiently decreased in moderate heart failure and their ratio (Bcl-2/Bax) was not significantly altered. Caspase-3 gene expression increased in heart failure. Compared to the control group, expression of Bax and Bcl-2 proteins and their ratio were increased in dogs after 4 weeks of pacing, but not after 7 weeks of pacing. No band of activated caspase was found in the normal nor in the paced myocardium. In the TUNEL assay there was no significant difference between numbers of apoptotic cells in all groups, although a few TUNEL-positive cells were detected in the dogs paced for 4 and 7 weeks. Conclusion: Altogether and in spite of previous studies our results are not in favour of apoptosis as a major mechanism in the pathogenesis of heart failure.
E Poster – [A-10-1352-1] High prevalence of metabolic syndrome in Iran in comparison to France What are the explanatory components? M. Azimi-Nezhada, Herbetha, G. Siesta, H. Esmailib, S.J. Hosseinic, M. Ghayour-Mobarhanc, S. Visvikis-Siestaa a Unité de recherche “Génétique Cardiovasculaire”, EA-4373, Université Henri Poincaré-Nancy 1, Faculté de Pharmacie, 30, rue Lionnois, 54000 Nancy, France b Department of Biostatistics and Epidemiology, Faculty of Health, Mashhad University of Medical Sciences, 9138813944 Mashhad, Iran c Biochemistry and Nutrition Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, 9138813944 Mashhad, Iran E-mail address: [email protected] (M. Azimi-Nezhad) Introduction: Several studies have shown a high prevalence of metabolic syndrome (MetS) and low HDL-C levels in Iranian populations. The aim of this study was to investigate the differences in the prevalence of MetS and of its components between an Iranian and a French population and give hypotheses for the origin of these differences. Methods and results: Prevalence of MetS and related abnormalities was estimated according to ATPIII definition in 1386 adults from France and 1194 adults from Iran of both sexes. MetS prevalence was the highest in Iranian women (55.0%), followed by Iranian men (30.1%), French men (13.7%) and French women (6.6%); all the prevalences were significantly different from each other. Iranian women were characterized by high rates of abdominal obesity (65.0%), hypertension (52.1%), hypertriglyceridemia (43.1%) and low HDL-cholesterol (92.7%). Iranian men were characterized by high rates of hypertension (48.9%), hypertriglyceridemia (42.8%) and low HDL-cholesterol (81.8%). French men had high rates of hypertension (44.7%) and mild rates of hypertriglyceridemia (28.6%) and hyperglycemia (23.9%). Pearson correlation coefficients between waist circumference and other MetS components were generally stronger in French individuals in comparison to Iranian, whatever the sex, for systolic blood pressure, HDL-cholesterol and fasting glucose and in French men only for triglycerides and diastolic blood pressure. Conclusion: The main finding of this study is a high prevalence of low HDL-C concentration in the Iranian population especially in Iranian women compared to French women. Other differences in metabolic syndrome criteria between the two countries may be mainly due to the environmental differences including life style, physical activity, dietary pattern and probably genetic factors of the two populations. Keywords: Metabolic syndrome prevalence, Metabolic syndrome component, Waist, Blood pressure, HDL cholesterol, Triglyceride, Glucose, Age, Country differences, Iran, France doi:10.1016/j.clinbiochem.2011.08.135
Histochemistry E Poster – [A-10-85-1] Comparison differentiated osteoblast from adipose stem cells and normal osteoblast Jafary Farzanehab, Esmaeili Nafiseha, Sadeghi Farzaneha, Jafary Faribaa a Department of Biology, Faculty of Basic Sciences, Alzahra University, Tehran, Iran
S66
Abstracts
b
Department of Anatomical Sciences, School of Medicine, Isfahan University of Medicine Sciences, Isfahan, Iran E-mail address: [email protected] (J. Farzaneh) Introduction: Bone is a strange tissue playing critical role in vital organs, blood production, mineral storage and homeostasis, blood PH setting and others. Bone tissue consists of mineralized components [such as calcium-phosphate mineral called hydroxyapatite], marrow, nerves, blood vessels, cartilage and three types of cells: osteoblast, osteocytes and osteoclasts. Osteoblasts are cells that have differentiated from mesenchymal stem cells and important roles in produce and secretes organic and inorganic bone matrix. Therefore osteoblast is a requisite cell in produced extra cellular matrix and regeneration of bone in many diseases such osteoporosis, osteomyelitis, osteoarthritis. Thus differentiation of osteoblast from stem cell is the main subject in tissue engineering. Method: In this study mesenchymal stem cells were isolated from adipose tissues by enzymatic digestive and were differentiated to osteoblast using osteogenic medium then these cells were caparisoned in protein levels, mineral matrix and morphology with normal osteoblasts were isolated from calvarias. Result: This result indicated that protein and mineral material production were not significant different in normal and differentiated osteoblast cells. Conclusion: Therefore according to the result we can use differentiated osteoblast from stem cell instead of bone transplant or nature osteoblast in bone defects. Keywords: Stem cell, Osteoblast, Cell differentiated doi:10.1016/j.clinbiochem.2011.08.136
E Poster – [A-10-782-1] Distribution pattern of some glycoconjugates of hair follicle regeneration in rat Saeed Modaghegh Samane Razavi Hospital, Mashhad, Iran E-mail address: [email protected] Introduction: Regeneration of skin wounds that cause alopsia is one of human problems after accidents or diseases. So introduction of effective drugs or procedures for hair follicle regeneration is important. Tissue regeneration occurs due to cell to cell interactions via the presence of macromolecules such as glycoconjugates that act as cellular receptors in biological events. The aim of present research was identification of some glycoconjugates of regenerating hair follicle. Methods: Ten Sprague rats were anesthetized with pentobarbital intraperitoneally and skin wounds of 4 mm were induced on each rat. These tissues were used as nature intact samples. Repairing wounds were sampled each 5 days until 50th day of post surgery. Paraffin sections were stained with PAS and Alcian blue pH 1, pH 2.5. In microscopic observation color intensity of staining was reported in tables (according to 0–5 gradient). Result: Pas and Alcian blue positive responses were observable (weak intensity) from 15th day in fibrous root sheath and dermal papilla of follicles. High color intensity was seen gradually until 40th day. Conclusion: In this research neutral, sulfated and carboxylated acidic glycoproteins appeared increasingly from mid stage of repairing in some layers of hair follicle. This specific stage distribution is probably related to different cellular activities that improve healing processes. Understanding of molecular aspects of tissue regeneration may be used in pharmacology or tissue engineering. Keywords: Hair follicle, Regeneration, Glycoconjugates
E Poster – [A-10-765-1] The effects of sodium metasilicate on the spinal motoneuron recovery following sciatic nerve injury Nikravesh Mohammad Reza Mashhad University of Medical Sciences, Iran E-mail address: [email protected] Introduction: The anti-oxidant effects of sodium metasilicate (SM) inhibit many oxidative enzymes in liver and kidney. The goal of this research was to study the protective effects of SM on the central degeneration of alpha motoneuron (AM) in the ventral horn of lumber spinal cord of rats which underwent compressive sciatic nerve lesion. Material and methods: The lesioned rats were divided into control, sham and experimental groups and the experimental group treated by 6 mg/kg.ip SM. The rats were kept for a month and then were sacrificed and perfused cardially by 10% formaldehyde and the lumber segments of their spinal cord dissected and sampled. After histological processing, by using a stereological technique, the dissector, the numerical density (Nv) of AM in the ventral horn of lumber spinal cord was estimated in all groups. Results and conclusion: The results showed that although the Nv of AM of sham group is significantly lower than the control (p < 0.001), the Nv of AM of experimental group is higher than shams (p < 0.05). Moreover there is no significant difference between experimental group and control. These results indicate that administration of SM has a beneficial effect on the survival of AM and may prevent or delay the central degeneration. Keywords: Sodium metasilicate, Spinal motoneurons, Sciatic nerve
doi:10.1016/j.clinbiochem.2011.08.137
doi:10.1016/j.clinbiochem.2011.08.138
E Poster – [A-10-785-1] The comparison of the mesenchymal progenitor cell coating state on the MBA and DBM as the biochemical scaffolds Hashemi Zahra Sadat, Forouzandeh Mahdi, Soleimani Masoud Medical Sciences,Tarbiat Modares University, Tehran, Iran E-mail addresses: [email protected] (H.Z. Sadat), [email protected] (F. Mahdi), [email protected] (S. Masoud) Introduction: 3D cultures in the tissue engineering field are a rapidly growing research by using biochemical material as scaffold. These biochemical materials are biocompatible to improve efficiency in the culture. These are used as a delivery vehicle. The most commonly used scaffolds in the bone marrow and cartilage are MBA and DBM. These are based on the bone matrix but the only difference between them is the lack of mineralized materials in DBM by the acid extraction method. Our goal is to understand how important is the presence of the mineral materials for the coating of the cells. Materials and methods: Human placenta mesenchymal progenitor cells were isolated and characterized. For 8 weeks, these cells were cultured in the specific media. Consequently the two scaffolds were immersed in the media culture for 2 hours. After that, cells were added. This suspension was rotated for 30 minutes during the incubation. After 3 days, the media was changed and the granules of two MPC-coating scaffolds were sent for Scanning Electron Microscope. Results: SEM images show the better coating on the demineralized bone matrix (DBM). We had nearly 50% coating cells on the DBM but 30% for MBA (mineralized bone allograft).