Comparison of field desorption and Fast Atom Bombardment mass spectrometry

International

Journal of Mass Spectrometry

and Ioon Physics,

46 (1983)

379

379-382

Elsetier Scientific Publishing Company, Amsterdam - Printed in The Netherlands

OF

COkPARISON

FIELD

AND

DESORPTION

FAST

BOMBARDMENT

ATOM

MASS

SPECTkOMETRY

J.

uer

van

Greef

and

Institutes

CIVO

ten

M.C.

TNO,

Noever

de

Box

360,

P.O.

Brauw 3700

AJ

Zeist

(The

Netherianas)

A6 STRACT Investigations carried out to compare fast atom bombardment in positive and negative ion mode - combined w%th low or high resolution - with field desorption snowed that the former technique is more easily feasible. It proauces more fragment ions as compared with field aesorption, which is a but a drawback in favouraole aspect as regards structure elucidation, the difference in information obtained with mixture analysis. Considering tne two tecnniques, it seems more practicable to use a combination than to apply only one of them.

INTRODUCTIOh Field 'soft

desorption

the

analysis

for

reasons

of

for

pared

to

sults

than

the

largely to

number

it

a

are

also

a

well

known

successfully Fast

been

(ref. of

FABMS

in

atom

proved

FDMS

in

analytical

this

bom-

suitable

the

near

of

the com-

better

that

future

re-

FABMS (ref.

of with

obtained

will Tn

2).

applicability

results

is

as

produce

to

the

One

simplicity

its

suggested

into

technique

publications. is

therefore

insight

l),

seems

FABMS

was

better

several

FABIVIS,

of

cases

supersede

obtain

techniques

increasing

and

used

compounds. has

recently

some

being

is

compounds.

popularity

FDMS

is

only

In

(FDMS)

polar (FABMS)

these

an

FDMS.

probably

and

of

introduced

subject

order

spectrometry

analysis

the

that

non-volatile

of

mass

Although the

technique

ionization'

bardment

spectrometry

mass

FDMS oath

discussed.

METHODS FD

and

Finnigan

System

Spectra and

FAB MAT

data

spectra 212 SS

mass 100

were

obtained

with

spectrometer, and

SS

200

a

Varian

MAT

were

used

for

731 A

respectively. data

acquisition

processing_

0020-7381/83/0000-0000/$03.00

0

and

Finnigan

1983 ElsevierScientific J?ublishingCompany

a

380

KESltLTS Identification

anu

quailtit?itiOri

of

1

solution

A FAB

in

mode

tne

positive

(-FD,-FAb)(ref.

were

obtaineii

ana

Na2F

+

for

ions

peaks

+

87

-FAn

+FAB

%

ground isotope

These

With it

was

hampered

by

a

the and

signals

currents

for

Na+

this

about

of

peaks

of

only

dominating

1:4('0

6=19.8

%,

pg.

The

glycerol

the

I0 B

back-

containing

87

197

(BF4-L

TNa(BF4121-,

provide

much

-FD

Moreover

can

and

250

the

and

and

glycerol

('"BF4-)

m/z

not

of

-FD

and two

.

-FAB

anionic

Na+,

only

aDout

and

does

quantitation

that

86

BF41-,

salt.

the

witn

of

[Na2(BFG)3]-

+FAB,

of +

NazBFq]

limit

183)

+FD

of

signals

peaKs:

307

whereas

to

found

ion

+FC

m/z

ratio

that

show

respect

at

+

and

information

salt.

and

negative

spectrum

tollowing

identification

provide

FD

lasting

strong

simple

[Glycerol

information,

the

ion

sy

the

+

-FD

91

the

results

teristic

High

detection

89,

179

[Glycerol-H+NaBF4]-

A

besides

peaks,

201

short

in

a a

71,

[NaBF5]-,

and

[Glycerol

contains

129

and

ions.

with

with

59,

analyzed

+FAB)

weak

+

ions.

in

(m/z

was

(+FD,

j+,

and

)

salt

resulted

obtained

spectrum

3)

iM+Naj

a NaBF4

observed.

( l'8Fq-)

-5=8".2

for

Only

Na21SFL:~+

is

m/z

3).

iNa2BF4

tilycerol

ot

moae

witn

Na2F+,

of

PI-'

ion

the

were

Experiments

iz

119

are

both

be

uSed

ppb

at

of

-FAB the

levels

since

background

and

part

at

useful

+FA&

cationic

BF4-

charac-

very

(ref.

+/-FAB

these

are

low

con-

centrations.

Detection

of

F DNS

an

organoarsenic

has

(CH3)++CH2COO-) (ref.

Hecent marine

weak

very

LM+H]'

ions

of

other

spectrum

of

pure a

contains

few

However,

no

significant

(0.1-l

in

more

provides

isolated

oe

products

and

tions

can

were

detected.

fragment

too

This

products

as

cases

was

due

no to

The spectrum,

compared

to

or the

'FAB but

the

+FD

information.

obtained

since

from

isolated some

+FD

structural be

plaice

fractions. the

peaks

could

low.

in

isolated

more

spectra marine

that

resembles

therefore

from Vg)

showed

the

from

fractions

on 5)

arsenobetaine

spectrum

tities

(ref.

arsenabetaine

isolated

measurements

+FD

identify

to

fractions

products

presence

it

used in

4).

various

COInpOUnd

been

the

from available

the

fracquan-

381

problem

if

situated hign

in

the

protonated The

at

m/z

using

to

record

in

found,

of LM+H]*

i.e.

doublet

arising analyzed

obtained

at

for

the

this

as

of

of

fractions

which

this

The

fraction

problem, analyzer around

[2Fi+H]+

isolated

Figure

in

1.

the

peak

high gave

of

FD

crab were

peaks

an

unresolved

Various

resolution poor

from

Two

and

background.

and

is

reference.

a

glycerol

interest

region

arsenobetaine

method

a

multichannel mass

179.0053.

stiown

peak

from by

m/z

especially

of

circumvent

counting

\nias used

are

is

region

relevant

analysis

way

the To

ion

the

ion

the

tnis

-

region. an

185.1OiS

results

obtained

were

FAE

molecular

glycerol

case

this mass

I ow

used

was

i 11

as

resolution

system

be

-

background

glycerol

witn

interference

The

fractions

data

could

even

results.

.. *s

c5 “12 02 179.M53

‘.

.._

i .,. . .: ..

-_

-:..

Figure

1:

High from

Analysis

of

Interesting study tration given

of by

using

isomeric

fumaric

acid acid).

of

was in

acids of

(trans-butenedioic +FD

an

fraction

isolated

analyzer.

obtained

behaviour

Upon

a

carboxylic

carboxylic

differences

(cis-butenedioic

analysis

a multichannel

information

unsaturated

on

FAB

unsaturated

‘.. I..

.

resolution crab

I_

__ .. z,:;:

‘.,

M+*

acids with

(ref. cis

FD 6).

and acid)

peak

for

and A

good

trans and

FAD

in illus-

isomers maleic

fumaric

a

acid

is acid

382 and

LM+Hj+

an

reflects

the

explained

by

carboxylic This

the

aifference

and

acid

upon

simi

mode fumaric

Iat-

acid

at

results is

strong

high

be

bound

is

also

(M+Hj+ fUmariC

is

can

spectrum

of

fumaric

be

the in

twc

gives negative

-FAB

acid

no

its

+FAB-spectrum.

-f for

the

the

than

both

observed

hardly

In but

better

shown

acid

obtainer,

considerably

which

between

peak

concentrations.

are

result

This

acid

sigion

spectrum l

+FAB

the are

even

found.

isomer. isomers

wnereas

+FAB,

peaks,

can

cis two

A

was maleic

of

proton

the

the

experiments.

nificant

a

in

between

-FAB

maleic

that

groups

acid

affinity

proton fact

acid

maleic

for

peak

higher

indication

of

M

of In

.

ions

found.

CONCLUSIONS The

application

easier

is

and

presence FD

not

more

a

+FAB

analysis.

observed

with

in merits

an

by

This

which

can

background the

Moreover,

selective

effected

fumaric

by

are

acid,

true

in be

used

as

with by

not

references.

FD, a

of

to

2 3 4 5 6

FAB is

a in

components heating.

In

affinity, occur

as

with

high

applied

at

compounds. it

may

be

the

field

of

and

defects.

observed polar

that non-volatile

FD

and

FAB

-

as

compounds

REFERENCES 1

be

disadvantage

proton

expected

might

which

controlled low

the

compounds.

with but

for is

but

other

mode

also

respect

resorption

FD

caused

holds

ion

this

of

compared

determination

negative

prob’tems,

presence

structure

be

and

advantage

as

problems

weight

conclusion

present own

can

experiments

molecular

analysis.

fragmentions in

mixture

FD

severe

strongly

advantage

mixture

positive

peaks

by

ion

in

while

solvent

more

generates

in

the hampereti

influenced clear

than

work,

of

is

FAB

faster

resolution

hign

In

of

U. J. Surman and J.C. Vickerman, J. Chem. Sot. Chem. Commun., (1981) 324 M. Barber, B.S. Bordoli, R.D. Sedgwick and A.N. Tyler, ibid (lYB1) 325. Bradley, G. Williams, C. Bojesen, S. Santikarn and D.H. L.C.E. Taylor, 3. Am. Ch-em. Sac. 103 (1981) 5700. 3. van der Greef, M-C.. Ten Noever de Brauw, J.J. Zwinselman and N.M.M. Nibbering, Biomed, Mass Spectrom., in press. J.B. Luten, G. Riekwel-Booy and A. Rauchbaar, in preparation. J_ van der Greef and M.C. Ten J.B. Luten, G, Riekwel-3ooy, Noever de Brauw, in preparation. J.W. Dallinga, N.M.M. Nibbering, J. van der Greef and M-C. Ten Noever de Brauw, in preparation.

have

their