- Email: [email protected]
Comparison of routine diagnostic thyrotropin assays: ELISA and Chemiluminescence assay
Abstracts
via binding to the nucleation site within the hydrophobic region of amylin (FGAIL). Conclusion: Although the mechanism by which EUK-8 and EUK134 inhibit amyloid formation from IAPP in vitro remains unclear; they could be key compounds for the development of therapeutics for T2DM. Keywords: Islet amyloid polypeptide (IAPP), Amyloid formation, EUK-8, EUK-134, 20–29 fragment
doi:10.1016/j.clinbiochem.2011.08.106
E Poster – [A-10-588-3] Effect of azithromycin, a chemical macrolide in reducing tissue destruction of patients with chronic periodontitis Banihashem Rad Seyed Ali, Mohammad Hasan Najafi Nesheli, Forouzanfar Ali Department of Periodontology, School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran E-mail addresses: [email protected] (B.R.S. Ali), [email protected] (M.H. Najafi Nesheli), [email protected] (F. Ali) Introduction: Chronic periodontitis is a common infectious disease of the mouth affecting the periodontal tissues. Its primary etiology is bacterial plaque, which can initiate destruction of the gingival tissues and periodontal attachment apparatus. Azithrymicin is a macrolid antibiotic which has been used in the treatment of several bacterial infections. The aim of this study was to evaluate the effect of azithromycin followed by scaling and roots planning (SRP) in the treatment of chronic periodontitis to introduce an adjunctive therapy to reduce tissue destruction in periodontal patients. Materials and methods: Forty nine patients with chronic periodontitis took part in this double-blinded randomized clinical trial. Patients of the test group received 500 mg azithromycin oraly one day before SRP and then continued it as 250 mg per day for four days. In control group placebo was applied. Periodontal indices; such as pocket depth (PD), clinical attachment loss (CAL), gingival index (GI) and plaque index (PI) have been evaluated in each patient before and 3, 13, and 25 weeks after treatment and data analyzed by statistical tests. Results: The results of this study demonstrated that using azithromycin in conjuction with SRP reduced tissue destruction resulted by chronic periodontitis. Conclusion: Azithromycin is active against gram positive organisms; it is excreted in high concentrations in saliva and is used as an adjunct to periodontal treatment. This study showed periodontal benefits, as measured by the gingival index, the plaque index and pocket depth. In addition, it is a safe, nontoxic drug with few and infrequent side effects. Keywords: Azithromycin, Chronic, Periodontitis doi:10.1016/j.clinbiochem.2011.08.107
E Poster – [A-10-591-2] Comparison of routine diagnostic thyrotropin assays: ELISA and Chemiluminescence assay Beiranvand Asghar, Rostami Rahim, Nouroozzadeh Jafar Medical Faculty-Naslu-Urmia-West Azarbaijan, Iran E-mail addresses: [email protected] (B. Asghar), [email protected] (R. Rahim), [email protected] (N. Jafar)
S51
Introduction: Thyroid dysfunctions are among common health problems. Thyrotropin (TSH) measurement is the first line thyroid function test. Since the 1990's new generations of fully automated TSH assay methods have been introduced. However, they are not feasible in many lab centers yet. Objective: Comparing ELISA and Chemiluminescence assays for TSH measurement. Methods: TSH values for 89 pregnant women(<12th week) were measured using two common trade ELISA kits against chemiluminescence assay as the gold standard. Results: TSH mean(CI95%), using chemilominescence method, was 1.32(1.13-1.5)IU/mL. the respected values for kit A and B were 1.07 (0.89-1.26)IU/mL, 2.1(1.74-2.45)IU/mL in order. ELISA method results showed good correlation with chemiluminescence results (P < 0.001). Assuming the central 95% of TSH values as normal range, kappa contingency coefficients were 0.65 and 0.27 for kit A and B respectively. Positive and negative predicted values for kit A were 100% and 98% respectively. The respected values for kit B were 15.3% and 100%. Conclusion: The importance of lab method precision, especially about common tests like TSH, highlights the necessity of evaluating the simple and cost-effective assays. Our findings revealed better concordance between kit A and the gold standard. Newly developed lab methods are not available at considerable part of health system centers. Therefore, more studies are needed to evaluate common at hand methods. Keywords: TSH, ELISA, Chemiluminescence, Diagnosis doi:10.1016/j.clinbiochem.2011.08.108
E Poster – [A-10-602-1] Association of serum antibody titers against heat shock protein 27 and prooxidant- antioxidant balance with the severity of coronary artery disease and metabolic syndrome Sahebkar Amirhossein, Ghayour-Mobarhan Majid, Pourghadamyari Hossein, Moohebati Mohsen, Parizadeh Seyyed Mohammad Reza, Tavallaie Shima, Paydar Roghayeh, Ferns Gordon Mashhad University of Medical Sciences, Mashhad, Iran E-mail address: [email protected] (S. Amirhossein) Introduction: Antibody titers to several heat shock proteins (antiHsps) and oxidative stress have been reported to be associated with the severity and progression of cardiovascular disease. However, there are little data regarding anti-Hsp27 titers and pro-oxidant antioxidant balance (PAB) in patients with coronary artery disease (CAD) and metabolic syndrome. Materials and methods: A total of 400 patients with suspected CAD were recruited. Based on the results of coronary angiography, these patients were classified into CAD+ (n = 300) and CAD- (n= 100) groups defined as patients with ≥50% and <50% stenosis of any major coronary artery, respectively. Eighty three healthy subjects were also recruited as the control group. Results: CAD+ patients had significantly higher anti-Hsp27 titers and PAB compared to both CAD- and control groups. Anti-Hsp27 titers and PAB were also higher in the CAD- group compared to the control group. With regard to the number of affected vessels in the CAD+ group, patients with 3 vessel disease (3VD) had higher anti-Hsp27 titers compared to both 2VD and 1VD subgroups, PAB did not differ significantly among patients with different vascular disease (SVD, 2VD and 3VD). Furthermore, the CAD+ patients were divided into metabolic syndrome and non-metabolic syndrome patients. Metabolic syndrome patients had significantly higher anti-HSP27 titer than non metabolic syndrome patients (p= 0.021). However, PAB did not differ significantly between them (p= 0.85).
via binding to the nucleation site within the hydrophobic region of amylin (FGAIL). Conclusion: Although the mechanism by which EUK-8 and EUK134 inhibit amyloid formation from IAPP in vitro remains unclear; they could be key compounds for the development of therapeutics for T2DM. Keywords: Islet amyloid polypeptide (IAPP), Amyloid formation, EUK-8, EUK-134, 20–29 fragment
doi:10.1016/j.clinbiochem.2011.08.106
E Poster – [A-10-588-3] Effect of azithromycin, a chemical macrolide in reducing tissue destruction of patients with chronic periodontitis Banihashem Rad Seyed Ali, Mohammad Hasan Najafi Nesheli, Forouzanfar Ali Department of Periodontology, School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran E-mail addresses: [email protected] (B.R.S. Ali), [email protected] (M.H. Najafi Nesheli), [email protected] (F. Ali) Introduction: Chronic periodontitis is a common infectious disease of the mouth affecting the periodontal tissues. Its primary etiology is bacterial plaque, which can initiate destruction of the gingival tissues and periodontal attachment apparatus. Azithrymicin is a macrolid antibiotic which has been used in the treatment of several bacterial infections. The aim of this study was to evaluate the effect of azithromycin followed by scaling and roots planning (SRP) in the treatment of chronic periodontitis to introduce an adjunctive therapy to reduce tissue destruction in periodontal patients. Materials and methods: Forty nine patients with chronic periodontitis took part in this double-blinded randomized clinical trial. Patients of the test group received 500 mg azithromycin oraly one day before SRP and then continued it as 250 mg per day for four days. In control group placebo was applied. Periodontal indices; such as pocket depth (PD), clinical attachment loss (CAL), gingival index (GI) and plaque index (PI) have been evaluated in each patient before and 3, 13, and 25 weeks after treatment and data analyzed by statistical tests. Results: The results of this study demonstrated that using azithromycin in conjuction with SRP reduced tissue destruction resulted by chronic periodontitis. Conclusion: Azithromycin is active against gram positive organisms; it is excreted in high concentrations in saliva and is used as an adjunct to periodontal treatment. This study showed periodontal benefits, as measured by the gingival index, the plaque index and pocket depth. In addition, it is a safe, nontoxic drug with few and infrequent side effects. Keywords: Azithromycin, Chronic, Periodontitis doi:10.1016/j.clinbiochem.2011.08.107
E Poster – [A-10-591-2] Comparison of routine diagnostic thyrotropin assays: ELISA and Chemiluminescence assay Beiranvand Asghar, Rostami Rahim, Nouroozzadeh Jafar Medical Faculty-Naslu-Urmia-West Azarbaijan, Iran E-mail addresses: [email protected] (B. Asghar), [email protected] (R. Rahim), [email protected] (N. Jafar)
S51
Introduction: Thyroid dysfunctions are among common health problems. Thyrotropin (TSH) measurement is the first line thyroid function test. Since the 1990's new generations of fully automated TSH assay methods have been introduced. However, they are not feasible in many lab centers yet. Objective: Comparing ELISA and Chemiluminescence assays for TSH measurement. Methods: TSH values for 89 pregnant women(<12th week) were measured using two common trade ELISA kits against chemiluminescence assay as the gold standard. Results: TSH mean(CI95%), using chemilominescence method, was 1.32(1.13-1.5)IU/mL. the respected values for kit A and B were 1.07 (0.89-1.26)IU/mL, 2.1(1.74-2.45)IU/mL in order. ELISA method results showed good correlation with chemiluminescence results (P < 0.001). Assuming the central 95% of TSH values as normal range, kappa contingency coefficients were 0.65 and 0.27 for kit A and B respectively. Positive and negative predicted values for kit A were 100% and 98% respectively. The respected values for kit B were 15.3% and 100%. Conclusion: The importance of lab method precision, especially about common tests like TSH, highlights the necessity of evaluating the simple and cost-effective assays. Our findings revealed better concordance between kit A and the gold standard. Newly developed lab methods are not available at considerable part of health system centers. Therefore, more studies are needed to evaluate common at hand methods. Keywords: TSH, ELISA, Chemiluminescence, Diagnosis doi:10.1016/j.clinbiochem.2011.08.108
E Poster – [A-10-602-1] Association of serum antibody titers against heat shock protein 27 and prooxidant- antioxidant balance with the severity of coronary artery disease and metabolic syndrome Sahebkar Amirhossein, Ghayour-Mobarhan Majid, Pourghadamyari Hossein, Moohebati Mohsen, Parizadeh Seyyed Mohammad Reza, Tavallaie Shima, Paydar Roghayeh, Ferns Gordon Mashhad University of Medical Sciences, Mashhad, Iran E-mail address: [email protected] (S. Amirhossein) Introduction: Antibody titers to several heat shock proteins (antiHsps) and oxidative stress have been reported to be associated with the severity and progression of cardiovascular disease. However, there are little data regarding anti-Hsp27 titers and pro-oxidant antioxidant balance (PAB) in patients with coronary artery disease (CAD) and metabolic syndrome. Materials and methods: A total of 400 patients with suspected CAD were recruited. Based on the results of coronary angiography, these patients were classified into CAD+ (n = 300) and CAD- (n= 100) groups defined as patients with ≥50% and <50% stenosis of any major coronary artery, respectively. Eighty three healthy subjects were also recruited as the control group. Results: CAD+ patients had significantly higher anti-Hsp27 titers and PAB compared to both CAD- and control groups. Anti-Hsp27 titers and PAB were also higher in the CAD- group compared to the control group. With regard to the number of affected vessels in the CAD+ group, patients with 3 vessel disease (3VD) had higher anti-Hsp27 titers compared to both 2VD and 1VD subgroups, PAB did not differ significantly among patients with different vascular disease (SVD, 2VD and 3VD). Furthermore, the CAD+ patients were divided into metabolic syndrome and non-metabolic syndrome patients. Metabolic syndrome patients had significantly higher anti-HSP27 titer than non metabolic syndrome patients (p= 0.021). However, PAB did not differ significantly between them (p= 0.85).