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Abstracts / Journal of Biotechnology 256S (2017) S17–S43
cis-regulation for hotspot activity, and despite being influenced by the trans-factor PRDM9, it is not the major regulator for this hotspot. http://dx.doi.org/10.1016/j.jbiotec.2017.06.650 Medicine & Biotechnology Computerized extraction of BI-RADS breast mass location descriptors from breast volume segmented on MR images Dogan Deniz Demirgunes 1,∗ , Gokhan Ertas 2 , Ziya Telatar 3 , Osman Erogul 4 1 Biomedical Engineering Center, Gulhane Training and Research Hospital, Ankara, Turkey 2 Department of Biomedical Engineering, Yeditepe University, I˙ stanbul, Turkey 3 Department of Electric and Electronics Engineering, Ankara University, Ankara, Turkey 4 Department of Biomedical Engineering, TOBB ETU University, Ankara, Turkey
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[email protected] (D.D. Demirgunes). With the recent advances in the field of computer aided detection (CAD) systems for breast MRI, computerized evaluations of the breasts aiming breast density assessment or lesion localization have been commonly used in the breast clinics. However, there is a need for computerized systems to report the findings according to the Breast Imaging-Reporting and Data System MRI lexicon developed by the American College of Radiology. In this study, we present a framework to computerize the extraction of breast mass location descriptors required in BI-RADS for MR-Mammography. This framework uses breast volume segmented on MR images as an input and outputs breast mass location descriptors automatically. The descriptors are the breast (left or right breast), the quadrant and the clockface in which the lesion is present, and the distance and the depth of the lesion from the nipple. Experiments on breast MR images with artificially placed breast masses show that the framework potentially provides a beneficial computerized tool adjunct to computer aided evaluations at breast clinics. http://dx.doi.org/10.1016/j.jbiotec.2017.06.651
Computer-based method for measuring the anteversion of the acetabular component on pelvic radiographs Seda Sahin 1,∗ , Emin Akata 1 , Osman Erogul 2 , Hatice Tuba Sanal 3 , Cemil Yildiz 4 , Cengiz Tuncay 5 1
Department of Electrical and Electronics Engineering, Baskent University, Ankara, Turkey 2 Department of Biomedical Engineering, TOBB University of Economics and Technology, Ankara, Turkey 3 Department of Radiology, Health Sciences University, Gulhane Medical Faculty, Ankara, Turkey 4 Department of Orthopaedic and Traumatology, Health Sciences University, Gulhane Medical Faculty, Turkey 5 Department of Orthopedics and Traumatology, Baskent University Hospital, Ankara, Turkey E-mail address:
[email protected] (S. Sahin). Malposition of the acetabular component is very important and has a preventable risk factor for the dislocation after Total Hip Artroplasty (THA). Therefore, the acetabular orientation and femoral orientation are crucial concepts of THA and to search the optimal placement of acetabular cup is the main aim for acetabular orientation. Besides the inclination angle, the anteversion angle is another significant parameter to determine the acetabular cup orientation. In this study, an automated anteversion angle measurement method is proposed to detect and validate the acetabular orientation after the surgery, to increase acetabular cup orientation on hip radiographs. The new method is applied on 30 hip radiographs and the manual measurements are performed by three clinicians and then results are compared with the automated method for each side of hip radiographs. The mean average difference between the manual method and automated method (leftside: 1.9 ± 3◦ ) (rightside: 2 ± 2.5◦ ) show that the proposed method can successfully be used for the measurement of anteversion angle after THA. http://dx.doi.org/10.1016/j.jbiotec.2017.06.652 Gene transfer studies by laboratory-made diode laser device Pinar Kuce Cevik 1,∗ , Gokhan Coral 2 , Ayla Celik 1 , Ibrahim Kucukkara 3 1 Faculty of Science and Letters, Department of Biology, Mersin University, Mersin, Turkey 2 Faculty of Science and Letters, Department of Biotechnology, Mersin University, Mersin, Turkey 3 Faculty of Science and Letters, Department of Physics, Mersin University, Mersin, Turkey
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[email protected] (P.K. Cevik). The aim of this study is to investigate of potential uses of diodelaser as gene transfer device in prokaryotic and eukaryotic cells. At 654 nm wavelength, 0.4-0.8-1.6-3.2 and 6.4 mW doses were used and exposure times were determined as 200, 400, 800, 1600 and 3200 ms for each dose. AMES, SCE, MN, Comet and cytotoxicity tests were performed to determine possible effects of laser light on the cells [1,2]. While pUC18 and phMGFP were used as vectors, E. coli JM109 and peripheral blood lymphocytes were used as recipients for transfection experiments. Transformed lymphocyte cells were determined by fluorescence attachment microscope and transformation efficiencies were calculated. -galactosidase