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Consistent chromosomal translocation in alveolar rhabdomyosarcoma
LETTER TO THE EDITOR Consistent Chromosomal Translocation in Alveolar Rhabdomyosarcoma
We performed a GTG- and RHG-banded chromosome analysis of cells from a metastatic ascitic effusion of an intraabdominal tumor in a 32-year-old male patient. Pathologic and electron microscopic diagnosis was alveolar r h a b d o m y o s a r c o m a (RS). In all the cells karyotyped, a reciprocal translocation involving band q37 of chromosome # 2 and band q14 of chromosome #13 was observed (Fig. 1); i.e., t(2;13)(q37;q14), according to international nomenclature [1]. A d d i t i o n a l chromosome changes were observed in a small proportion of the cells. The m o d a l chromosome n u m b e r was 46. Only four cases of RS with chromosome banding patterns have been p u b l i s h e d [2-5]. With regard to their histologic types [6], two were of the embryonal subtype [2, 5] and one was not specified [4]. Though n u m e r o u s chromosome changes were reported in each of these three cases, no consistent features were observed. The fourth case was classified as an alveolar subtype [3]. Among other anomalies, a marker 2 q + was described and interpreted as the result of a t(2;13)(q37;q14) with subsequent loss of the der(13). This translocation was similar to the one found in our RS case, classified as alveolar subtype; we suggest that t(2;13)(q37;q14) may be a new consistent chromosome change in h u m a n tumors, characteristic of alveolar RS. It must be e m p h a s i z e d that band 13q14 involved in retinoblastoma was also involved in RS. CLAUDE TURC-CAREL SARAB LIZARD-NACOL EVE JUSTRABO
MARIE FAVROT THIERRY PHILIP ERIC TABONE
Facult~ de M~decine Laboratory of Cytogenetics Dijon, France Facult~ de M6decine Laboratory of Anatomopathology Dijon, France Centre L~on B~rard Department of Pediatrics Lyon, France Centre L~on B6rard Cellular Morphology Unit Lyon, France
Address requests for reprints to Dr. Claude Turc-Carel, Facult~ de M~decine, Laboratoire de Cytog~nOtique, 21033 Dijon Cedex, France. Received April 25, 1985; accepted May 29, 1985. 361 1986 Elsevier Science Publishing Co., Inc. 52 Vanderbilt Ave., New York, NY 10017
©
Cancer Genet Cytogenet 19:361-362(1986)
0165-4608/86/$03.50
362
( : T u r c - C a r e l e! al.
|
der2
der13 13
F i g u r e 1. Partial karyotype showing the rcp(2;13) (q37;q14) translocation in an alveolar rhabdomyosarcoma. (a) GTG-banded; (b) RHG-banded.
Supported by grants from La Ligue Bourguignonne contre le Cancer, L'Association pour la Recherche sur le Cancer, Villejuif.
REFERENCES 1. ISCN (1981): An International System for Human Cytogenetic Nomenclature. Birth Defects 17(5). 2. Nelson-Rees WA, Flandermeyer RR, Hawthorne PK (1975): Distinctive banded marker chromosomes of h u m a n tumor cell lines. Int J Cancer 16:74-82. 3. Seidal T, Mark J, Hagmar B, Angervall L (1982): Alveolar rhabdomyosarcoma: A cytogenetic and correlated cytological and histological study. Acta Path Microbiol l m n m n o l Scand Sect A 90:345-354. 4. Becher R, Wake N, Gibas Z, Ochi H, Sandberg AA (1984): Chromosome changes in soft tissue sarcomas. J Natl Cancer Inst 72:823-831. 5. Potluri VR, Gilbert F (1985): A cytogenetic study of embryonal rhabdomyosarcoma. Cancer Genet Cytogenet 14:169-173. 6. Enzinger FM, Lattes R, Torloni H (1969): Histological typing of soft tissue tumors. International Classification of Tumors No. 3, WHO, Geneva.
We performed a GTG- and RHG-banded chromosome analysis of cells from a metastatic ascitic effusion of an intraabdominal tumor in a 32-year-old male patient. Pathologic and electron microscopic diagnosis was alveolar r h a b d o m y o s a r c o m a (RS). In all the cells karyotyped, a reciprocal translocation involving band q37 of chromosome # 2 and band q14 of chromosome #13 was observed (Fig. 1); i.e., t(2;13)(q37;q14), according to international nomenclature [1]. A d d i t i o n a l chromosome changes were observed in a small proportion of the cells. The m o d a l chromosome n u m b e r was 46. Only four cases of RS with chromosome banding patterns have been p u b l i s h e d [2-5]. With regard to their histologic types [6], two were of the embryonal subtype [2, 5] and one was not specified [4]. Though n u m e r o u s chromosome changes were reported in each of these three cases, no consistent features were observed. The fourth case was classified as an alveolar subtype [3]. Among other anomalies, a marker 2 q + was described and interpreted as the result of a t(2;13)(q37;q14) with subsequent loss of the der(13). This translocation was similar to the one found in our RS case, classified as alveolar subtype; we suggest that t(2;13)(q37;q14) may be a new consistent chromosome change in h u m a n tumors, characteristic of alveolar RS. It must be e m p h a s i z e d that band 13q14 involved in retinoblastoma was also involved in RS. CLAUDE TURC-CAREL SARAB LIZARD-NACOL EVE JUSTRABO
MARIE FAVROT THIERRY PHILIP ERIC TABONE
Facult~ de M~decine Laboratory of Cytogenetics Dijon, France Facult~ de M6decine Laboratory of Anatomopathology Dijon, France Centre L~on B~rard Department of Pediatrics Lyon, France Centre L~on B6rard Cellular Morphology Unit Lyon, France
Address requests for reprints to Dr. Claude Turc-Carel, Facult~ de M~decine, Laboratoire de Cytog~nOtique, 21033 Dijon Cedex, France. Received April 25, 1985; accepted May 29, 1985. 361 1986 Elsevier Science Publishing Co., Inc. 52 Vanderbilt Ave., New York, NY 10017
©
Cancer Genet Cytogenet 19:361-362(1986)
0165-4608/86/$03.50
362
( : T u r c - C a r e l e! al.
|
der2
der13 13
F i g u r e 1. Partial karyotype showing the rcp(2;13) (q37;q14) translocation in an alveolar rhabdomyosarcoma. (a) GTG-banded; (b) RHG-banded.
Supported by grants from La Ligue Bourguignonne contre le Cancer, L'Association pour la Recherche sur le Cancer, Villejuif.
REFERENCES 1. ISCN (1981): An International System for Human Cytogenetic Nomenclature. Birth Defects 17(5). 2. Nelson-Rees WA, Flandermeyer RR, Hawthorne PK (1975): Distinctive banded marker chromosomes of h u m a n tumor cell lines. Int J Cancer 16:74-82. 3. Seidal T, Mark J, Hagmar B, Angervall L (1982): Alveolar rhabdomyosarcoma: A cytogenetic and correlated cytological and histological study. Acta Path Microbiol l m n m n o l Scand Sect A 90:345-354. 4. Becher R, Wake N, Gibas Z, Ochi H, Sandberg AA (1984): Chromosome changes in soft tissue sarcomas. J Natl Cancer Inst 72:823-831. 5. Potluri VR, Gilbert F (1985): A cytogenetic study of embryonal rhabdomyosarcoma. Cancer Genet Cytogenet 14:169-173. 6. Enzinger FM, Lattes R, Torloni H (1969): Histological typing of soft tissue tumors. International Classification of Tumors No. 3, WHO, Geneva.