Journal of Biochemical and Biophysical Method~. 22 (1991 ) 339--341 ~-;'.1991 Elsevier Science Publishers B.V. 0165-022X/91/503.50
339
Contents of Volume 22 No. I Review
S.E. Brolin and G. Wettermark (Sweden) Analytical applications of bioluminescence - a matter of proper kinetic design and recording
Research Articles
R.L.P. Adams, A. Rinaldi and C. Setwvright (UK) Microassay for D N A methyltransferase
19
R A&n, E.A. Bayer and M. Wilchek (Israel) A coupled enzyme assay for measurement of sialidase activity
23
D. Grenier (Canada) Assay for protease inhibitors: qualitative measurement and detection in polyacrylamide gel electrophoretograms
35
P.A. Janrney (L~S.A.) A torsion pendulum for measurement of the viscoelasticity of biopolymers and its application to actin networks
41
R.C. YashRoy (Canada) Protein heat denaturation and study of membrane lipid-protein interactions by spin label ESR
55
1. 5:afa~ik (Czechoslocakia) Spectrophotometric determination of amylase activity in colourcd soh, tions
61
IV. Daseler, II.-J. Steinhoff and A. Redhardt (F.R.G.) A new method for the determination of the permittivity of small samples in the microwave range and its application to hemoglobin single crystals
69
Short Notes
A.S.W. Li and C.F. Chignell (U.S.A.) The N o H value in EPR spin trapping: a new parameter for the identification of 5.5-dimethyl-1pyrroline-N-oxide spin adducts
83
D.J. Pillion, S.-J. Kim and E. Mee=an (U.S.A.) Increased sensitivity of 32p and lZSl counted in the presence of a solid scintillator: detection of protein bands separated by polyacrylamide gel electrophoresis
89
No. 2 Research Articles
J. Kambayashi, M. Yokota, M. Sakon, E. Shiba, T. KawasakL 7~ Mori, M. Tsacho'a, It. Oishi and S. Matsuura (Japan)
340 A novel endotoxin-specific assay by turbidimetry with Limulua" amoebocyte lysate containing fl-giucan
93
L.-E. Briggner and I. Wad~6 (Sweden) Test and calibration processes for microcalorimeters, with special reference to heat conduction instruments used with aqueous systems
101
R.J. Rouwenhorst, J. Frank Jzn, !4~d. Scheffers and J.P. Van Di/ken (The Netherlands) Determination of protein concentration by total organic carbon analysis
119
M. l'rihout, S. Paredes, J.M. Gonz6lez-?vlaiias and t':M. Gohi (Belgium, Spain) Binding of Triton X-100 to bovine serum albumin as studied by surface tension measurements
129
M. Scarpa, P. Vighno, F. Momo, lq Bracco, L. Battistin and A. Rigo (haly) NMR method for superoxide dismutasc assay in brain and liver homogenates
135
S.-P. Hm'n, R.W. Tilhnann, M. Egger and It.E. Gaub ([qR.G.) A miniaturized rmcro-fluorescence film balance for protein-containing lipid monolayers spread from a vesicle suspension
145
K.B. Hendil and [4~ Uerkvitz (Denmark) The human muhicatalytic proteinase: affinity purification using a monoclonal antibody
159
Short Notes J. Suh, B.K itwang, L Jang and E. Oh (Korea) Spectral titration of active site of carboxypeptidase A
167
N. Ramesh and R.K. Mishra (India) Screening of clones containing altered DNA conformations by plasmid topoprofile shift
171
A. Mir& M.T. lterndndez, M.J. Costas and d. (L Cameselle (Spain) Enzyme saturation and inhibition kinetics studied from multiple progress curves recorded spectrophotometrically from single reaction mixtures for ADP-ribose pyrophosphatase
I77
Announcement from the Publisher
iii
No. 3
Announcement from the Publisher
iii
Research Articles A. Breier, V. Duri.~ovdt, 7-. Stankovi~ov{~, D. Misloei~ovc~ and P. Gemeiner (Czechoslo~:akia) Application of adsorption kinetics for estimation of dissociation constants
185
C Vasseur, .~: Galacteros, P. Groff and ti. Wajcman (France, Luxembourg) Cleavage by protease from Staphylococcus aureus VS: an improvement in the sequence analysis of human hemoglobin variants
195
M. L.M. Anderson, D.A. Spandidos and J.R. Coggins ( U. K , Greece) Electroporation of lymphoid cells: factors affecting the efficiency of transfection
207
G.E. Wise and bi Lm (L(S.A.) Transfer of silver-stained proteins from polyacrylamide gels to pol3Mnylidene difluoride membranes
223
341 P.F. Lindholm, S.J. Marriott, S.D. Gitlin and J.N. Brad)' (U.S.A.) Differential precipitation and zinc chelate chromatography' purification of biologically active t-ITI,V-I l a x 1 expressed in E. coli
233
G. Tigvi and I. Parker (US.A.) Microinjection into Xenop~9 oocytes: a precise semi-automatic instrument and optimal parameters for injection of mRNAs
243
7£ llisabori, K lnoue, Y. Akahane. S. hvakami and K. Manahe (Japan) Two-dimensional gel clectrophoresis of the membrane-bound protein complexes, including photosystem 1. of thylakoid membranes in the presence of sodium oligooxyethylene alkyl ether sulfate/dimcthyl dodecylamine oxide and sodium dodecyl sulfate
253
Announcement
261
No. 4
Announcement from the Publisher
iii
Research Articles
H. Kajiwara, tl. Hirano and K. Oono (Japan) Binding shift assay of parvalbumin, calmodulin and carbonic anhydrase by high-performance capillary electrophorcsis
263
E. l~ Vandenberg and U.J. Krull (Canadaj Tile prevention of adsorption of interferents to radiolabelled protein by Tween 20
269
M. Guthridge, J. Bertolini, J. Schmitt and M.T.W. Hearn (Australia) Detection of FGF-/3 mRNA in chondrosarcoma cells by a new in situ hybridization technique with synthetic oligonncleotide probes
279
D.-P. lliider and K. Vogel (F.R.G.) Interactive image analysis system to determine the motility and velocity' of cyanobacterial filaments
289
M.J. l,arnmi and M, Tamrni (Finland) Autoradiographic quantitation of radiolabeled proteoglyeans
301
J.-p. 1,i and S. tljertFn (Sweden) High-performance liquid chromatography of proteins on deformed nonporous agarose beads: immuno-affinity chromatography, exemplified with human growth hormone as ligand and a combination of ethylene glycol and salt for desorption of the antibodies
311
L. ttolmquist and M. Lewin (Sweden) Separation of glutaraldehyde and some of its aldol condensation products by hydroxyl-aldehyde group affinity chromatography
321
Short Note
P. LOnnbro and 1. WadsO (Sweden) Effect of dimethyl sulphoxide and some antibiotics on cultured human T-lymphoma cells as measured by microcalorimetry
331
Announcements
337
Contents of Volume 22
339
Author Index
343
Subject Index
347