Contents volume 33

THERIOGENOLOGY

CONTENTS VOLUME

Number

1,

January

33

1990

JOHN DENNIS BIGGERS, RECIPIENT OF THE 1990 EMBRYO TRANSFER PIONEER AWARD . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1 SYMPOSIUM

ON QUALITY CONTROL IN EMBRYO TRANSFER J.F. Hasler, Chairman

QUALITY CONTROL IN A LARGE-SCALE EMBRYO TRANSFER PROGRAM UNDER FARM CONDITIONS IN THE ARGENTINE REPUBLIC: QUALITY CONTROL FACTORS AND RESULTS C.J. Munar, M.A. Nigro, E.R. Burry, R.A. Vautier and C. Argerich . . . . . . . . . . . . . . . . . . . . . . . . . .5 QUALITY CONTROL MEASURES IN AN EMBRYO RESEARCH PROGRAM M.C. Schiewe, P.M. Schmidt, D.E. Wildt and W.F. Ball . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9 QUALITY CONTROL IN THE IN VITRO FERTILIZATION LABORATORY W.R. Boone and S.S. Shapiro . . . . . . . . . . . . . . . . . . . . . . . . . .23 DESIGN ANALYSIS OF RESEARCH ON INFECTIOUS DISEASE TRANSMISSION BY EMBRYOS W.C.D. Hare . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .51 CERTIFICATION IN THE AMERICAN EMBRYO TRANSFER ASSOCIATION R.D. Baker and J.R. Webb . . . . . . . . . . . . . . . . . . . . . . . . . . . . .59 OVA AND EMBRYOS FOR QUALITY CONTROL PROCEDURES IN REPRODUCTIVE BIOLOGY S.P. Leibo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . . ..67 (continued)

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THERIOGENOLOGY MAIN PROGRAM N.L. First, Chairman; J. Parrish,

Assistant

Chairman

PRODUCTION OF EMBRYOS IN VITRO AND ITS IMPACT ON LIVESTOCK PRODUCTION I. Gordon and K.H. Lu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .77 DEVELOPMENTAL CAPACITY OF MOUSE OOCYTES THAT GROW AND MATURE IN CULTURE: THE EFFECT OF MODIFICATION OF THE PROTOCOL J.J. Eppig, A.C. Schroeder, J.J. van de Sandt, C.A. Ziomek and B.D. Bavister . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .89 NEW TECHNIQUES FOR ASSISTED FERTILIZATION C. Reefer . . . . . . . . . . .. . . . . . . ..**.*...................

101

METHODS OF GENE TRANSFER AND THEIR POTENTIAL USE TO MODIFY MILK COMPOSITION I. Wilmut, A.L. Archibald, S. Harris, M. McClenaghan, J.P. Simons, C.B.A. Whitelaw and A.J. Clark......................113 DERIVATION AND PRELIMINARY CHARACTERIZATION OF PLURIPOTENT CELL LINES FROM PORCINE AND BOVINE BLASTOCYSTS M.J. Evans, E.. Notarianni, S. Laurie and R.M. Moor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .125 PUBLIC POLICY ASPECTS OF PATENTING TRANSGENIC ANIMALS L.J. Raines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .129 EARLY CLEAVAGE AND THE MATERNAL ZYGOTIC TRANSITION IN BOVINE EMBRYOS F.L. Barnes and W.H. Eyestone....................... FACTORS AFFECTING THE VIABILITY OF NUCLEAR TRANSPLANTED EMBRYOS L.C. Smith and I. Wilmut . . . . . . . . . . . . . . . . . . . . . . . . . . . .153 PRODUCTION OF IDENTICAL BOVINE OFFSPRING BY NUCLEAR TRANSFER K.R. Bondioli, M.E. Westhusin and C.R. Looney . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .165 MATERNAL RECOGNITION OF PREGNANCY AND EMBRYONIC LOSS R.M. Roberts, T. Schalue-Francis, H. Francis and D. Keisler . . . . . . . . . . . . . . . . . . . . . . . . . . .175

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*Denotes abstracts

ABSTRACTS entered in student competition

DIFFERRENCES IN TYPE OF MITOCHONDRIAL DNA (mtDNA) BETWEEN DONORS AND RECIPIENTS DO NOT AFFECT PREGNANCY RATES AFTER TRANSFER OF RAT EMBRYOS A.A. Alcivar and M.H. Pineda........................ SUPEROVULATION USING DIFFERENT DOSES OF PMSG AND MONOCLONAL ANTI-PMSG IN CATTLE M.M. Alfuraiji, P.J. Broadbent, J.S.M. Hutchinson, D.F. Dolman and T. Atkinson . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ..186 SYNTHESIS AND RELEASE OF PEPTIDES PRODUCED BY EQUINE TROPHOBLASTIC VESICLES IN VITRO M. Altschul, G. Ignotz, W.B. Currie and B.A. Ball . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .187 SIZE DISTRIBUTION AND MEIOTIC COMPETENCE OF BOVINE PRIMARY OOCYTES FROM TWO LOCATIONS IN THE OVARY *T.M. Arlotto, M.L. Leibfried-Rutledge and N.L. First . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .188 IN VITRO DEVELOPMENT OF 4- TO 8-CELL EQUINE EMBRYOS COCULTURED WITH TROPHOBLASTIC VESICLES OR OVIDUCTAL EXPLANTS B.A. Ball and M. Altschul . . . . . . . . . . . . . . . . . . . . . . . . . . .189 CERVICAL EMBRYO COLLECTION IN SHEEP AFTER RIPENING OF THE CERVIX WITH PROSTAGLANDIN E2 AND ESTRADIOL *D.M. Barry, C.H. van Niekerk, J. Rust and T. van der Walt . . . . . . . . . . . . ..*.................. 190 IMMUNOCYTOCHEMICAL EVIDENCE THAT LH AND FSH ARE PRODUCED BY DISTINCT CELLS IN BOVINE PITUITARY E. Bastings, A. Beckers, M. Resnik, F. Ectors and J.F. Beckers . . . . . . . . . . . . . . . . . . . . . . . . . .191 ARE PORCINE FOLLICLE STIMULATING HORMONE ANTIBODIES ASSOCIATED WITH DECREASED SUPEROVULATORY RESPONSE IN THE GOAT? J.F. Beckers, G. Baril, J.C. Vallet, D. Chupin, B. Remy and J. Saumande..................l92

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THERIOGENOLOGY DEVELOPMENT AND VIABILITY OF ONE- AND TWO-CELL PORCINE EMBRYOS CULTURED THROUGH THE "FOUR-CELL BLOCK" *L.s. Beckmann, T.C. Cantley, A.R. Rieke and B.N. Day .. . . . . . . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .193 ZONA-PELLUCIDA MICROSURGERY USING A NITROGEN LASER D.D. Beetem, D. Huffman and R.A. Godke..............l94 DEVELOPMENTAL RATES OF IN VITRO PRODUCED IVM-IVF BOVINE OOCYTES IN DIFFERENT CELL CO-CULTURE SYSTEMS U. Berg and G. Brem . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .195 ONE STEP FREEZING OF RABBIT EMBRYOS B. Zhu, G. Wang, B. Ma and J. Wang..................196 LIVE BIRTHS FOLLOWING TRANSFER OF CAPRINE EMBRYOS CULTURED FOR 72 HOURS IN DOMESTIC CHICKEN EGGS E.G. Blakewood, J.F. Prichard, S.H. Pool and R.A. Godke . . . . . . . . . . . . . . . . . . . . . . . . . . . .197 THE EFFECT OF SYNCHRO-MATE-B EAR IMPLANT AND ESTRADIOL TREATMENT ON SUPEROWLATORY RESPONSE IN THE COW *G.A. Bo, A. Garcia, F. Schumann, R.A. Pierson and R.J. Mapletoft.....................l98 THE 50% FRACTION OF BOVINE SPERMATOZOA WHICH SELECTIVELY BINDS PHYTOHAEMAGGLUTININ (PHA-L) DOES NOT CORRELATE WITH SEX GENOTYPE M.P. Bradley, K.I. Matthaei, C.M. Herr, S. Beaton and K.C. Reed . . . . . . . . . . . . . . . . . . . . . . . . . . . . .199 THE EFFECTS OF MONOJECT AND AIR-TITE SYRINGES ON EQUINE SPERMATOZOA J.R. Broussard, J.D. Roussel, M. Hibbard, J.K. Thibodeaux, J.D. Moreau, S.D. Goodeaux and L.L. Goodeaux . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .200 SUPEROVULATION IN DALL'S SHEEP (Ovis dalli dalli) B.C. Buckrell, C.J. Gartley, K.G. Mehren and K.L. Goodrowe . . . . . . . . . . . . . . . . . . . . . . . ..*......... 201 A PRELIMINARY REPORT ON THE EFFICACY OF BIODEGRADABLE MICROSPHERES FOR THE CONTROLLED RELEASE OF PROGESTERONE AND ESTRADIOL FOR SYNCHRONIZATION OF OVULATION IN MARES P.J. Burns, B.A. Ball, T.R. Tice, D.W. Mason and D.F. Love . . . . . . . . . . . . . . . . . . . . . . . . . . . .202

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THERIOGENOLOGY EFFECT OF INSULIN ON GLUCOSE UPTAKE BY MURINE EMBRYOS IN LOW GLUCOSE MEDIUM J.E. Butler and J.E. Williams.......................203 MURINE EMBRYO DEVELOPMENT IN VITRO: EFFECT OF THERMAL STRESS ON BLASTOCYSTS J.S. Capehart and K.L. White........................204 EFFECT OF PROTEIN SUPPLEMENTS ON IN VITRO MATURATION, FERTILIZATION AND CULTURE TO BLASTOCYST AND HATCHING STAGE OF BOVINE OOCYTES H.B. Chen-Lu and K.H. Lu . . . . . . . . . . . . . . . . . . . . . . .. . . . .205 EFFECT OF CRYOPRESERVATION MEDIUM AND STRAW SIZE ON POST-THAW SURVIVAL OF MOUSE EMBRYOS IN VITRO G.D. Corbin, C.E. Pope and B.L. Dresser.............206 INTRAVULVO-SUBMUCOSAL INJECTIONS OF LUPROSITIOL MAY REACH CORPORA LUTEA BY A LOCAL, UNILATERAL PATHWAY IN CATTLE S.A. Cordova, F.K. Jimenez and A. Villa-Godoy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .207 SUPEROVULATION WITH THREE DIFFERENT COMMERCIAL PITUITARY EXTRACTS IN THE COW M.R. Del Campo, F. Becerra, M. Gonzalez, B.D. Murphy and R.J. Mapletoft......................208 THAWING AND GLYCEROL REMOVAL FROM FROZEN BOVINE EMBRYOS M.R. Del Campo, F. Becerra and R.J. Mapletoft . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .209 PERIOVULATORY ENDOCRINE PROFILE IN MURRAH BUFFALOES UNDER TWO SUPEROVULATORY REGIMES L. Deshpande, S.P. Singal, G.C. Georgie and 1-S. Lohan......................... BEHAVIOR OF THYMOCYTE NUCLEI WHEN TRANSPLANTED INTO MATURING MOUSE OOCYTES B.A. Didion and C.L. Markert........................211 INFLUENCE OF POLYETHYLENE GLYCOL ON MOTILITY AND ACROSOME REACTION OF RABBIT AND MOUSE SPERM B.A. Didion and C.N. Graves.........................212 DEVELOPMENT OF VITRIFIED RABBIT EMBRYOS J.R. Dobrinsky, J.J. Balise and J.M. RobI........... EMBRYO PRODUCTION BY SUPER-OV AND FSH-P L.E. Donaldson . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .214

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THERIOGENOLOGY FSH-P BATCH VARIATION L.E. Donaldson . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .215 VESICLES AS A MARKER FOR BOVINE EMBRYO QUALITY M. Dorland, H. de Boer-Brouwer, T. Otter, J.M.C. Reinders, A.A. Bosma and Th.A.M. Kruip . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .216 SUPEROVULATION AND EMBRYO RECOVERY IN THE AMERICAN BISON (Bison bison) C.G. Dorn, W.B. Foxworth, P.D. Butler, G.C. Olsen, B.A. Wolfe, D.S. Davis, T.R. Simpson and D.C. Kraemer.......................217 MAINTENANCE OF CALVING INTERVAL IN SUPEROVULATED CATTLE C.G. Dorn, L.A. Martin, J.M. Farr and D.C. Kraemer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .218 XENOGENOUS FERTILIZATION OF GOAT OVULATED OOCYTES IN THE RABBIT OVIDUCT E.K. Duan, G.Y. Wang, B.H. Ma and J.C. Wang . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .219 PARTIAL CHARACTERIZATION OF THE IMMUNOSUPPRESSIVE PROPERTIES OF PREGNANCY-SPECIFIC PROTEIN B (PSPB) *M.M. Dunbar, T.S. Wong, C.A. Ruder-Montgomery, B.P. Chew and R.G. Sasser . . . . . . . . . . . . . . . . . . . . . . . . . . .220 SEMEN CHARACTERISTICS OF THE PRZEWALSKI'S STALLION (Equus przewalskii) B.S. Durrant . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .221 RELATIONSHIPS AMONG CIRCULATING CONCENTRATIONS OF INHIBIN, FSH AND LH IN CATTLE AT ESTRUS S.E. Echternkamp and B.D. Schanbacher...............222 IN VITRO DEVELOPMENTAL POTENTIAL OF BOVINE ZYGOTES IN OVIDUCT EPITHELIAL CELL CO-CULTURE SYSTEMS J.E. Ellington, P.B. Farrell, E.W. Carney, M.E. Simkin and R.H. Foote . . . . . . . . . . . . . . . . . . . . . . . . . .223 BOVINE ZYGOTE DEVELOPMENT IN OVIDUCT CELL COCULTURE VERSUS RABBIT OVIDUCTS J.E. Ellington, P.B. Farrell, M.E. Simkin, E.E. Goldman and R.H. Foote.........................224 THE USE OF A GNRH ANALOG IN AN EMBRYO TRANSFER FIELD TRIAL J. Ellington, R. Foote, J. Webb, J. Hasler and A. McGrath . . . . . . . . . . . . . . . . . . . . . . . . . . . .225

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THERIOGENOLOGY THE USE OF OVIDUCT-CONDITIONED MEDIUM FOR CULTURE OF BOVINE OOCYTES TO THE BLASTOCYST STAGE W.H. Eyestone, J.M. Jones and N.L. First............226 GALACTOSYLTRANSFERASE CONCENTRATIONS IN THE PLASMA MEMBRANES OF BOVINE AND EQUINE SPERM CELLS R.A. Fayrer-Hosken, B.D. Shur and A.B. Caudle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ..227 AN EVALUATION OF MEIOTIC STAGES AND POLYSPERMY IN UNCLEAVED OOCYTES FOLLOWING IN VITRO FERTILIZATION IN DOMESTIC CATS E.J. Gelwicks, C.E. Pope, J.L. Turner, G.L. Keller and B.L. Dresser........................228 USE OF PMSG PLUS NEUTRA-PMSG IN DAIRY COWS TREATED REPEATEDLY FOR SUPEROWLATION J.Th. Gielen, G.H. Roerink, R.E. Atoon, C.A. Vonk Noordegraaf, J. Pasman, M.J.H. Hoeijmakers, R.H.M. Steeg, A. Coert, D. Aguer and T. Nell......................229 COMPARISON OF A PROGESTERONE INTRAVAGINAL DEVICE (CIDR) WITH FLUNIXINE MEGLUMINE (FINADYNE) FOR REDUCING THE EFFECTS OF CORPORA LUTEA REGRESSION IN THE GOAT D.E. Gilbert, S.A. Coonrod, C.J. Whiting and R.L. Pashen . . . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .230 AN ATTEMPT TO SUPEROVULATE A SOUTHERN WHITE RHINOCEROS (Ceratotherium simum simum) R.W. Godfrey, C.E. Pope, B.L. Dresser, B.D. Bavister, J.C. Andrews and J.H. Olson..........231 SUPEROVULATION AND EMBRYO COLLECTION IN JAMUNAPARI GOATS A.K. Goel and K.P. Agrawal . . . . . . . . . . . . . . . . . . . . . . . . . .232 EVALUATIONN OF A NONSURGICAL UTERINE FLUSHING TECHNIQUE IN SIX SPECIES OF NONHUMAN PRIMATES L.L. Goodeaux, C.A. Anzalone, R.A. Lowstetter, M.R. Cranfield, J.D. Moreau, J.K. Thibodeaux and J.D. ROUSSel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .233 NONSURGICAL COLLECTION AND TRANSFER OF RHESUS MONKEY EMBRYOS L.L. Goodeaux, C.A. Anzalone, S.A. Voelkel, Y. Menezo, J.K. Thibodeaux and J.D. ROUSSel....................234

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THERIOGENOLOGY THE EFFECT OF CMRL-1066 AND MENEZO B2 MEDIA ON COCULTURE OF RHESUS EMBRYOS L.L. Goodeaux, C.A. Anzalone, S.A. Voelkel, Y. Menezo, J.K. Thibodeaux and J.D. Roussel....................235 DEVELOPMENTAL STAGES OF MACACA MULATTA EMBRYOS BASED ON DAY OF NONSURGICAL COLLECTION L.L. Goodeaux, C.A. Anzalone, S.A. Voelkel, J.K. Thibodeaux and J.D. RousSel . . . . . .. . . . . . . . . . . . . . . . . ..*.......... 236 MEIOTIC MATURATION OF DOMESTIC CAT OVARIAN OOCYTES IN VITRO K.L. Goodrowe, K.G. Mehren and W.A. King............237 FERTILIZATION BY SPERM INJECTION IN CATTLE K. Goto, A. Kinoshita, Y. Takuma and K. Ogawa . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ..- 238 SUPEROVULATION IN HEIFERS GIVEN FSH INITIATED EITHER AT DAY 2 OR AT MID LUTEAL PHASE OF THE OESTROUS CYCLE *D. Goulding, D.H. Williams, P. Duify, M.P. Boland and J. F. Roche.........................239 INFLUENCE OF A GNRH ANALOGUE ON FOLLICULAR DYNAMICS IN COWS PRETREATED OR NOT WITH FSH-P L.A. Guilbault, J.G. Lussier, F. Grass0 and P. Matton . . . . . . . . . . . . . . . . . . . .. . . . . . . . .240 ESTRUS SYNCHRONIZATION AND SUPEROVULATION OF MATURE RATS G.S. Hamilton and D.T. Armstrong....................241 DEEP FREEZING OF MURINE I-CELL EMBRYOS AND OOCYTES J.J. Hernandez-Ledezma and R.W. Wright, Jr..........242 FREEZING RATES AND CRYOPROTECTANT INTERACTIONS ON THE CRYOPRESERVATION OF EARLY MOUSE BLASTOCYSTS J.J. Hernandez-Ledezma and R.W. Wright,

Jr..........243

INCREASED NUMBER OF PREGNANCIES PER COLLECTED EMBRYO BY BISSECTION OF BLASTOCYST STAGE OVINE EMBRYOS C. Herr, N. Holt. Y. Pietrzak, K. Old and K. Reed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ..244

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THERIOGENOLOGY A RAPID Y-CHROMOSOME-DETECTING OVINE EMBRYO SEXING ASSAY C.M. Herr, K.I. Matthaei, U. Petrzak and K.C. Reed . . . . . . . . . . . . . . . . . . . . . . . . . . . .245 RAPID Y-CHROMOSOME-ASSAY SEXING OF PERIPHERAL BLOOD LYMPHOCYTES FROM BOVINAE OF KNOWN PHENOTYPIC SEX C.M. Herr, K.I. Matthaei, T. Steel and K.C. Reed . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . .246 SEX OF PROGENY FROM BOVINE EMBRYOS SEXED WITH A RAPID Y-CHROMOSOME-DETECTION ASSAY C.M. Herr, N.A. Holt, K.I. Matthaei and K.C. Reed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .247 A TRIAL TO STIMULATE INSULIN LIKE GROWTH FACTOR 1 LEVELS TO IMPROVE SUPEROVULATORY RESPONSE IN DAIRY COWS A. Herrler, E. Farries and H. Niemann...............248 BULL SPECIFIC EFFECT ON FERTILIZATION AND EMBRYO DEVELOPMENT IN VITRO F.L Hillery, J.J. Parrish and N.L. First............249 EMBRYO TRANSFER IN DANISH ANGORA GOATS P. Holm, B.A. Petersen, K. Krogh, F. Dagnaes-Hansen and I.A. Laursen..................250 TRANSFER OF ANGORA GOAT EMBRYOS IMPORTED INTO DENMARK FROM NEW ZEALAND UNDER QUARANTINE CONDITIONS P. Holm, B.A. Petersen, J. Hepburn, K. Krogh, F. Dagnaes-Hansen and H. Callesen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .251 TRANSCRIPTIONAL ACTIVITY OF PORCINE BLASTOMERE NUCLEI INTRODUCED INTO IN VITRO MATURED OOPLASM P. Hyttel, R. Prochazka, S. Smith, J. Kanka, T. Greve and R. Hoier.....................252 THE EFFECT OF PMSG ANTIPMSG ON THE PERFORMANCE OF RABBIT EMBRYOS M.J. Illera, C. Rodriguez de Sadia, I. Munoz and M. Illera . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .253 DEVELOPMENT OF IN VITRO FERTILIZED BOVINE EMBRYOS IN THE COW UTERUS H. Itakura, K. Kameyama, T. Takeda and T. Onihara . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .254 LOW DOSES OF CALCIUM IONOPHORE A23187 STIMULATE MOTILITY IN MURINE SPERMATOZOA K.A. Ivani and G.E. Seidel, Jr......................255

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THERIOGENOLOGY EFFECT OF GONADOTROPIN PRIMING ON THE SUPEROVULATORY ESTRUS AND PLASMA PROGESTERONE PROFILE AMONG BUFFALOES S. Jailkhani, B.S. Prakash, S.K. Singla and M.L. Madan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ...256 BOVINE AMNIOTIC AND ALLANTOIC FLUIDS FOR THE CULTURE OF MURINE EMBRYOS M.H. Javed and R.W. Wright, Jr......................257 EFFECTS OF PMSG, INSULIN, OSMOLALITY AND OESTROUS COW SERUM ON DEVELOPMENT OF IVF EARLY BOVINE EMBRYOS CULTURED ON GRANULOSA CELL MONOLAYERS H.S. Jiang, W.L. Wang, K.H. Lu and I. Gordon........258 THE USE OF ULTRASONOGRAPHY TO INCREASE RECIPIENT EFFICIENCY THROUGH EARLY PREGNANCY DIAGNOSIS A.L. Jones, D.E. Marek, J.M. Wilson and C.R. Looney . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259 EXPERIMENTS ON IN VITRO CAPACITATION OF EJACULATED SPERM AND IN VITRO FERTILIZATION IN THE RABBIT X. Jun, Q. Jufen, Z. Yong and W. Jianchen...........260 PREGNANCY SUCCESS IN NORMAL CYCLING MICE BRED AFTER EXPOSURE TO RU486 S.C. Juneja, M.G. Dodson and J.L. Robertson.........261 SEMEN CRYOPRESERVATION AND THE FIRST SUCCESSFUL ARTIFICIAL INSEMINATION OF GAUR (Bos gaurus) S.M. Junior, D.L. Armstrong, S.H. Hopkins, L.G. Simmons, M.C. Schiewe and T.S. Gross...........262 IN VITRO MATURATION OF BOVINE OOCYTES FOLLOWING BUFFER MICROINJECTION INTO GERMINAL VESICLE OR CYTOPLASM J. Jura, Z. Smorag, L. Katska and M. Bak............263 PREGNANCY RATES AND BIRTHS AFTER CO-CULTURE OF CUMULUS CELLS WITH BOVINE EMBRYOS DERIVED FROM IN VITRO FERTILIZATION OF IN-VITRO MATURED FOLLICULAR OOCYTES Y. Kajihara, N. Kometani, S. Kobayashi, Y. Shitanaka, Y. Koshiba, K. Hishiyama, K. Shiraiwa and K. Goto . . . . . . . . . . . . . . . . . . . . . . . . . . . . .264 EFFECTS OF STORAGE METHODS OF FETAL CALF SERUM ON THE DEVELOPMENT OF MOUSE EMBRYOS K. Kameyama, T. Takeda and T. Onihara...............265

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THERIOGENOLOGY MURINE EMBRYO BIOPSY & FULL-TERM DEVELOPMENT FOLLOWING TRANSFER OF BIOPSIED EMBRYOS J.G. Kim, W.E. Roudebush, M.G. Dodson and B.S. Minhas . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .266 STUDIES ON THE IN VITRO MATURATION AND FERTILIZATION RATE OF BOVINE FOLLICULAR OOCYTES S.K. Kim and H.K. Park . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .267 STUDIES IN THE PRODUCTION OF CHIMAERIC CATTLE EMBRYOS BY AGGREGATION OF BLASTOMERES FROM EMBRYOS DERIVED FROM OOCYTES MATURED AND FERTILIZED IN VITRO A. Kinis, E. Vergos, I. Gordon, A. Gordon and M. Gallagher.......................

268

INCREASED SUCCESS RATE OF IVM AND IVF IN THE BOVINE AFTER SONOGRAPHIC GUIDED TRANSVAGINAL COLLECTIONS OF THE OOCYTES Th.A.M. Kruip, M.C. Pieterse, Th.H. van Beneden, P. Vos, Y.A. Wurth and M.A.M. Taverne....................

269

FOLLICULAR DYNAMICS IN HEIFERS AFTER INJECTION OF PGF2 DURING THE FIRST WAVE OF FOLLICULAR DEVELOPMENT M. Lavoir and J.E. Fortune.......................

270

TRANSFER OF FRESH DAIRY EMBRYOS TO KOREAN NATIVE CATTLE AFTER LONG DISTANCE TRANSPORTATION IN KOREA K.W. Lee, D.S. Son, I.H. Kim and D.K. Jeon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .271 IN VITRO CULTURE AND CRYOPRESERVATION OF FARM ANIMALS' PRIMORDIAL GERM CELLS F. Leichthammer and G. Brem.........................272 EFFECT OF CYTOSKELETAL INHIBITOR ON FUSION AND DEVELOPMENT OF BOVINE NUCLEAR TRANSFER EMBRYOS M.J. Levanduski and M.E. Westhusin.....

............

273

RABBIT OVIDUCT EPITHELIAL CELL CULTURE AS A POSSIBLE MODEL FOR CAPACITATING SPERM J. Li, E. Carney, Y-Q. Chen, X. Yang and R.H. Foote . . . . . . . . . . . . . . . . . . . . . . . . . .......... ..27 4 FREQUENCY OF CLEAVAGE AND DEVELOPMENT IN VITRO OF BOVINE OOCYTES FERTILIZED IN DIFFERENT NUMBERS IN DROPS WITH DIFFERENT SPERM CONCENTRATIONS 971; Z.J. Ling and K.H. Lu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ..a..#

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THERIOGENOLOGY FACTORS INFLUENCING THE DEVELOPMENTAL COMPETENCE OF INTRASPECIFIC MURINE CHIMERAS PRODUCED BY MULTIPLE EMBRYO AGGREGATION N.M. Loskutoff and D.C. Kraemer.....................276 INTRASPECIFIC BLASTOCYST RECONSTITUTION IN MICE USING GIANT TROPHECTODERMAL VESICLES PRODUCED BY MULTIPLE EMBRYO AGGREGATION N.M. Loskutoff and D.C. Kraemer....................277 PREGNANCIES ESTABLISHED IN CATTLE BY TRANSFER OF FRESH AND FROZEN EMBRYOS DERIVED FROM IN VITRO MATURATION AND FERTILIZATION OF OOCYTES AND THEIR SUBSEQUENT CULTURE IN VITRO K.H. Lu, H.S. Jiang, W.L. Wang and I. Gordon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . . .278 ANTIBODY RESPONSES OF EWES TO CHIMERIC SHEEP <--) GOAT CONCEPTUSES ENCLQSED IN OVINE TROPHOBLAST *L.A. MacLaren, G.B. Anderson, R.H. BonDurant, T.L. Roth and D. Bernoco . . . . . . . . . . . . . . . . . . . . . . . . . . . .279 FACTORS LIMITING SUPEROVULATION RESPONSES IN EMBRYO TRANSFER PROGRAMME AMONG BUFFALOES M.L. Madan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .280 RESOLUTION OF CATTLE PEDIGREES BY DNA GENOTYPING D.A. Mann, K.I. Matthaei and K.C. Reed..............281 COMPARISON OF FOUR DIFFERENT GONADOTROPIN TREATMENTS FOR INDUCING SUPEROWLATION IN COWS WITH I:29 TRANSLOCATION R.J. Mapletoft, V. Pawlyshyn, A. Garcia, G.A. Bo, N. Willmott, J. Saunders and s. schmutz .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .282 NUCLEAR TRANSPLANTATION IN THE BOVINE: EFFECT OF DONOR EMBRYO AGE ON SUBSEQUENT EMBRYO PRODUCTION D.E. Marek, J.H. Pryor, T.H. Whitesell and C.R. Looney . . . . . . . . . . . . . ..*..................... 283 CHARACTERIZATION OF FERTILIZATION AND EARLY EMBRYONIC DEVELOPMENT OF NATURALLY OVULATED PIG OVA M.J. Martin, B.A. Didion and C.L. Markert...........284 ANALYSIS OF THE SEX CHROMOSOME CONSTITUTION OF SINGLE BOVINE SPERMATOZOA BY POLYMERASE CHAIN REACTION (PCR) K.I. Matthaei, C.M. Herr, M.P. Bradley and K.C. Reed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .285

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THERIOGENOLOGY CHANGES IN FOLLICULAR HORMONES AFTER PROSTAGLANDIN INJECTION IN SUPEROVULATED BEEF HEIFERS R.R. Maurer and T.H. Wise . . . . . . . . . . . . . . . . . ..*...*... 286 VITRIFICATION OF OVINE EMBRYOS L.K. McGinnis and C.R. Youngs.......................287 THE EFFECTS OF TIME-OF-EXPOSURE OF ALGINATECOATED RAT EMBRYOS TO CALCIUM CHLORIDE ON IN VITRO DEVELOPMENT AND PREGNANCY MAINTENANCE IN EWES WITH ALGINATE-COATED 4- OR 8-CELL EMBRYOS S. Meredith, D.K. Nicks and D.O. Kiesling...........288 MULTIPLE OVULATION AND EMBRYO TRANSFER IN INDIAN BUFFALOES (BUBALUS BUBALUS) A.K. Misra, B.V. Joshi, P.L. Agrawala, R. Kasiraj, S. Sivaiah, N.S. Randareddi and M.U. Siddiqui . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .289 THE USE OF HEMASTIX AS A DIAGNOSTIC AID IN THE DETECTION OF MENSTRUATION IN THE CERCOPITHECUS AETHIOPS AND MACACA MULATTA FEMALES J.D. Moreau, J.D. Roussel, J.K. Thibcdeaux, C.A. Anzalone and L.L. GoodeaUX . . . . . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . .290 THE EFFECT OF DIFFERENT MEDIA AND SERUM SUPPLEMENTS UPON PORCINE OOCYTE MATURATION Y. Nakanishi, Y. Uto, K. Goto and K. Ogawa..........291 EMBRYO TRANSFER TECHNOLOGY AS A TOOL TO ESTABLISH BOVINE GENETIC RESOURCES P. Nienhaus, B. Sacher, D. Smidt and H. Niemann . . . . . . . . . . . .. . . . . . . . . . . . . . . . . . . . . . . . . .292 EFFECTS OF FSH AND HEPARIN ON IN VITRO MATURATION, FERTILIZATION -AND DEVELOPMENT OF BOVINE OOCYTES *S.E. Olson, A. Romero, W.K. Thomas and G.E. Seidel, Jr . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .293 THE EFFECT OF METHODS OF THAWING AND GLYCEROL REMOVAL ON SURVIVAL OF FROZEN MOUSE AND BOVINE EMBRYOS A. Palasz, M.R. Del Campo and R.J. Mapletoft . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .294 SUBSTITUTION OF CHEMICALLY DEFINED MACROMOLECULES FOR PROTEIN IN MURINE EMBRYO FREEZING MEDIUM A. Palasz, S. Alkemade and R.J. Mapletoft...........295

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EFFECTS OF ACETATE, CITRATE AND MALATE ON DEVELOPMENT OF IN VITRO FERTILIZED BOVINE EMBRYOS T.T. Peura . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .296 THE USEFULNESS OF ULTRASONOGRAPHY IN COMPARISON WITH RECTAL PALPATION FOR THE DETECTION OF BOVINE CORPORA LUTEA M.C. Pieterse, M.A.M. Taverne, Th.A.M. Kruip and A.H. Willemse.....................297 EFFECTS OF ENDOTHELIAL CELL GROWTH FACTOR ON CULTURED AND CO-CULTURED PORCINE EMBRYOS J.L. Pipkin, S.D. Prien and J.R. Clark..............298 IN VITRO FERTILIZATION IN THE DOMESTIC CAT: EFFECT OF MEDIUM ON DEVELOPMENT DURING A FOUR-DAY CULTURE PERIOD IN VITRO C.E. Pope, E.J. Gelwicks, J.L. Turner, G.L.. Keller and B.L. Dresser.......................299 CULTURE OF EARLY-STAGE CAPRINE EMBRYOS USING GOAT OVIDUCTAL AND UTERINE CELL MONOLAYERS *J.F. Prichard, S.H. Pool, E.G. Blakewood and R.A. Godke . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .300 BEHAVIOR OF PIG BLASTOMERE NUCLEI INTRODUCED INTO IN VITRO MATURED OOPLASM BY ELECTRICALLY INDUCED FUSION R. Prochazka, S. Smith, P. HYttel and T. Greve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .301 REGULATED EXPRESSION OF THE MOUSE WHEY ACIDIC PROTEIN GENE IN TRANSGENIC SWINE V.G. Pursel, R.J. Wall, L. Hennighausen, C.W. Pittius and D. King . . . . .. . . . . . . . . . . . . . . . . . . . . . .302 CORRELATIONNS BETWEEN DAY FIVE MILK PROGESTERONE AND SUPEROVULATORY RESPONSE IN THE BOVINE V. Rainio . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .303 MICROINJECTION OF RECOMBINANT P-TRANSPOSABLE ELEMENTS INTO PRONUCLEI OF ONE-CELL MOUSE EMBRYOS M.L. Reed, L.C. Skow and D.C. Kraemer...............304 PRODUCTION OF CELL COLONIES FROM OVINE BLASTOCYSTS C.E. Rexroad, Jr . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .305

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THERIOGENOLOGY THE EFFECT OF CO-TREATMENT WITH RECOMBINANT BOVINE SOMATROPHIN (rBST) ON THE SUPEROVULATORY RESPONSE IN HOLSTEIN HEIFERS D. Rieger, J.S. Walton, M.L. Goodwin and W.H. Johnson . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .306 THE EFFECT OF TREATMENT WITH AN LHRH ANTAGONIST ON THE QUALITY OF DAY-7 EMBRYOS COLLECTED FROM SUPEROVULATED HOLSTEIN HEIFERS D. Rieger, J.S. Walton, W.H. Johnson and D.H. Coy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .307 BOVINE AND OVINE SUPEROWLATION RESPONSE TO FSH-P (LABORCLIN) PRODUCED IN BRAZIL J.L. Rodrigues, J.S. Stefani, M.D. Palha, L. Christmann, S.R. Lisboa and M.C. Silveira . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .308 A BILAYERED FETAL-CELL CO-CULTURE SYSTEM FOR CULTURING BOVINE EMBRYOS H.F. Rodriguez, K.W. Wiemer, R.S. Denniston and R.A. Godke.......................309 FOLLICULAR FLUID OBTAINED AFTER THE LH SURGE STIMULATES MEIOTIC MATURATION OF BOVINE OOCYTES *A. Romero, W.K. Thomas, S.E. Olson and G.E. Seidel, Jr . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .310 EFFECTS OF CULTURE ON THE POST-THAW VIABILITY OF CRYOPRESERVED, IN VITRO FERTILIZED BOVINE EMBRYOS R.W. Rorie, K.P. Xu and K.J. Betteridge.............311 SUPPRESSION OF MITOGEN-INDUCED HORSE LYMPHOCYTE PROLIFERATION BY PREIMPLANTATION HORSE CONCEPTUS-CONDITIONED MEDIUM T.L. Roth, K.L. White, D.L. Thompson Jr., B.E. Barry, J.S. Capehart, D.R. Colborn and M.H. Rabb . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . .312 INFLUENCE OF A DOMINANT FOLLICLE ATRESIA AND ESTRADIOL RELEASE BY OVARIAN FOLLICLES DURING SUPEROVULATION IN CATTLE *p. Rouillier, P. Matton, L. Guilbault, F. Grass0 and J. Lussier . . . . . . . . . . . . . . . . . . . . . . . . . . . .313 COMPARING EARLY EMBRYO MORTALITY IN DAIRY COWS DURING HOT AND COOL SEASONS OF THE YEAR D.P. Ryan, J.F. Prichard, E. Kopel and R.A. Godke . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .314

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THE USE OF FOLLICLE STIMULATING HORMONE (FSH) TO STIMULATE FOLLICLE DEVELOPMENT FOR IN VITRO FERTILIZATION DURING THE FIRST TRIMESTER OF PREGNANCY IN CATTLE *D.P. Ryan, E.G. Blakewood, W.F. Swanson, H. Rodriguez and R.A. Godke . . . . . . . . . . . . . . . . . . . . . . ...315 ARE FETAL CALF SERUM AND HORMONES NECESSARY DURING IN VITRO MATURATION OF CATTLE OOCYTES FOR SUBSEQUENT DEVELOPMENT K. Saeki, M.L. Leibfried-Rutledge and N.L. First . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .316 IN VITRO DEVELOPMENT OF BLASTOMERES ISOLATED FROM 8- AND 16-CELL PORCINE EMBRYOS ON EXTRACELLULAR MATRICES S. Saito and H. Niemann . . . . . . . . ..*.................. 317 STIMULATORY ACTION OF EPIDERMAL GROWTH FACTOR (EGF) ON BOVINE OOCYTE MATURATION IN SERUM FREE DEFINED MEDIUM A. Sanbuissho, S. Coskun and Y.C. Lin...............318 DOSE TITRATION OF FOLLTROPIN IN THE COW J. Saunders, N. Willmott, A. Palasz and R.J. Mapletoft . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .319 ENDOCRINE EVALUATION OF SUPEROVULATION IN WATER BUFFALOES E. Schallenberger, H.G. Wagner, R. Papa, P. Hart1 and H. Tenhumberg . . . . . . . . . . . . . . . . . . . . . . . . . .320 IN SITU STRAW DILUTION OF OVINE EMBRYOS CRYOPRESERVED BY CONVENTIONAL FREEZING OR VITRIFICATION M.C. Schiewe, W.F. Rall, L.D. Stuart and D.E. Wildt . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .321 CRYOPRESERVATION OF BOVINE EMBRYOS IN MEDIA WITH CHEMICALLY DEFINED MACROMOLECULES G.E. Seidel, Jr., R.P. Elsden and Z. Brink..........322 PRODUCTION OF HIGH QUALITY VIABLE BLASTOCYSTS FROM HAMSTER 8-CELL EMBRYOS USING HAMSTER EMBRYO CULTURE MEDIUM -2 P.B. Seshagiri and B.D. Bavister....................323 EFFECTS OF BULLS ON FERTILIZATION OF BOVINE OOCYTES AND THEIR SUBSEQUENT DEVELOPMENT IN VITRO D.S_ Shi, K.H. Lu and I. Gordon.....................324 ESTRUS BEHAVIOUR AND OVARIAN RESPONSE TO SUPEROWLATION AMONG PREPUBERAL BUFFALOES C. Singh and M.L. Madan . . . . . . . . . . . . . . . . . . . . . . . . . . . . .325

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PLASMA PROLACTIN IN RELATION TO ESTRUS INDUCTION DURING SYNCHRONIZATION AND SUPEROVULATION AMONG PERI-PUBERTAL BUFFALOES C. Singh and M.L. Madan . . . . . . . . . . . . . . . . . . . . . . . . . . . . .326 RESPONSE OF SUPEROVULATION IN BUFFALOES (Bubalus bubalis) WITH SUPER-OV AND FSH-P S.K. Singla and M.L. Madan . . . . . . . . . . . . . . . . . . . . . . . . . .327 COMPARATIVE SUPEROVULATORY RESPONSES IN CROSSBRED DAIRY CATTLE (Bos indicus x Bos taurus) WITH FSH-P AND SUPER-OV S.K. Singla and M.L. Madan . . . . . . . . . . . . . . . . . . . . . . .. . .328 SINGLE OVULATION AND EMBRYO TRANSFER (Soet) IN NON-SUPEROVULATED BUFFALOES S.K. Singla and M.L. Madan . ..****.*.................

329

LAPAROSCOPIC VERSUS SURGICAL TRANSFER OF OVINE EMBRYOS J.S. Stefani, M.D.C. Palha, L. Christmann, J.M. Rosa, M.C. Silveira and J.L. Rodrigues.........330 TRYPSIN TREATMENT OF BOVINE EMBRYOS AFTER IN VITRO EXPOSURE TO INFECTIOUS BOVINE RHINOTRACHEITIS VIRUS OR BOVINE HERPESVIRUSD.A. Stringfellow, L.H. Lauerman, K.B. Nasti and P.K. Galik . . . . . . . . . . . . . . . . . . . . .. . . . . .331 EVALUATION OF HUMAN AND BOVINE EMBRYOS BY MEASUREMENTS OF PAF IN THE CULTURE MEDIUM A.E. Stock, R. Axtell, H. Jones, G. Jones and W. Hansel . . . . . . . . . . . .. . . . . . . . . . . . . . . . . .332 THE EFFECT OF PENICILLAMINE, HYPOTAURINE, EPINEPHRINE AND SODIUM METABISULFITE ON BOVINE IN VITRO FERTILIZATION J.L. Susko-Parrish, M.B. Wheeler, R.L. Ax, N.L. First and J.J. Parrish................333 COMPARISON OF ONE STEP SUCROSE DILUTION AND DIRECT TRANSFER OF FROZEN EMBRYOS IN GLYCEROL 1,2_PROPANEDIOL T. Suzuki, M. Yamamoto, M. Ooe, A. Sakata and K. Matsuoka . . . . . . . . . . . . . . . . . . . . . . . . . . .334 EFFECTS OF DIFFERENT OESTRUS STAGES OF OVARIES AND SIZES OF FOLLICLES ON GENERATION OF BOVINE EMBRYOS IN VITRO S.J. Tan and K.H. Lu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .335

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THE EVALUATION OF PREGNANCY ASSOCIATED THROMBOCYTOPENIA AS AN INITIAL RESPONSE TO PREGNANCY IN CATTLE J.K. Thibodeaux, J.D. ROuSSel, R.W. Adkinson and L.L. Goodeaux.....................336 GNRH DOWN-REGULATION IN A SUPEROVULATION REGIME FOR NONHUMAN PRIMATES J.K. Thibcdeaux, J.D. Roussel, Y. Menezo, M.R. Cranfield, C.A. Anzalone, J.D. Moreau, R. Harrison and L.L. Goodeaux.......................337 HUMAN EMBRYOS EVALUATION BY ANALYSIS OF CULTURE MEDIA X. Tian, A. Stock, H. Jones, G. Jones and W. Hansel . . . . . . . . . . . . . . . . . . . . . . . . . .. . . .338 MEASUREMENT OF THE ACTIVITY OF THE PENTOSE PHOSPHATE PATHWAY IN SEXED BOVINE EMBRYOS G. Tiffin, D. Rieger, K.J. Betteridge, B.R. Yadav and W.A. King . . . . . . . . . . . . . . . . . . . . . . . . . . . .339 PROGESTERONE AND ESTROGEN PROFILE DURING SUPEROVULATION IN BUFFALOES D.K. Tuyen, Sujata Jailkhani, M.L. Madan, B.S. Prakash and S.K. Singla........................340 PRODUCTION OF CATTLE IDENTICAL TWINS BY SPLITTING BLASTOCYSTS USING A METAL MICROBLADE K. Utsumi and A. Iritani . . . . . . . . . . . . . . . . . . . . . . . . . . . .341 IN VITRO CULTURE OF SHEEP ZYGOTES FOLLOWING PRONUCLEAR MIOCROINJECTION S.K. Walker, T.M. Heard, P.J. Verma, C.S. Bawden, A.V. Sivaprasad, G.E. Rogers and R.F. Seamark . . . . . . . . . . . . . . . . . . . . . ...342 EFFECT OF CONDITION MEDIUM AND GLUCOSE CONCENTRATION ON THE IN VITRO DEVELOPMENT OF EARLY BOVINE EMBRYOS W.L. Wang, H.S. Jiang, K.H. Lu and I. Gordon........................ COCULTURE OVIDUCTAL J.A. D.K.

. ............ ...34 3

OF DAY-2 EQUINE EMBRYOS WITH OR UTERINE TISSUE Weber, D.A. Freeman, Vanderwall and G.L. Woods,...... . ........... ...34 4 4

EFFECT OF SERUM ON IN VITRO MATURATION OF EQUINE OOCYTES *p. Willis, R.A. Fayrer-Hosken and A.B. Caudle . . . . . . . . . . . . . . . . . . . . . . . ............ ..34 5

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THERIOGENOLOGY THE EFFECT OF BOVINE FOLLICULAR FLUID PRETREATMENT ON SUPEROVULATION IN THE COW *N. Willmott, J. Lussier, V. Pawlyshyn, A. Palasz, B.D. Murphy and R.J. Mapletoft...........346 THE EFFECT OF FSH/LH RATIO IN PITUITARY EXTRACTS ON SUPEROVULATORY RESPONSE IN THE COW N. Willmott, J. Saunders, G.A. Bo, A. Palasz, R.A. Pierson and R.J. Mapletoft..........347 CERVICAL SOFTENING WITH PROSTAGLANDIN E2 AND ESTRADIOL CYPIONATE FOR EMBRYO COLLECTION IN GOATS C.H. van Niekerk, D.M. Barry, J.M. Rust, T. van der Walt and J. Langenhoven..................348 INFLUENCE OF A DOMINANT FOLLICLE ON THE SUPEROVULATORY RESPONSE J.M. Wilson, A.L. Jones and D.R. Miller.............349 PREIMPLANTATION DEVELOPMENT OF EMBRYOS PRODUCED BY INTERGENERIC NUCLEAR TRANSPLANTATION B.A. Wolfe, M.E. Westhusin, M.J. Levanduski, K.R. Bondioli and D.C. Kraemer......................350 PREGNANCY RATES FOLLOWING TRANSFER OF BOVINE EMBRYOS PRODUCED BY IN VITRO MATURATION, FERTILIZATION AND CO-CULTURE K.P. Xu, J.W. Pollard, R.W. Rorie, L. Plante, W.A. King and K.J. Betteridge............351 IN VITRO FERTILIZATION (IVF) AND CULTURE (IVC) OF BOVINE OOCYTES FROM STORED OVARIES N.S. Yang, K.H. Lu and I. Gordon....................352 ISOLATION AND CULTURE OF SINGLE a-CELL BLASTOMERES IN RABITS WITH A NOVEL APPROACH X. Yang, L. Zhang, A. Kovacs, C. Tobback and R.H. Foote . . . . . . . . . . . . . . . . . . . . . . . . . . .353 PREGNANCY RATE IN THE RABBIT MODEL FOLLOWING HYPEROSMOTIC SUCROSE TREATMENT OF EMBRYOS X. Yang and R.H. Foote . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .354 THE INFLUENCE OF BOVINE OOCYTE TYPE ON IN VITRO FERTILIZATION AND SUBSEQUENT DEVELOPMENT IN VITRO Y.B. Yang and K.H. Lu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .355 SEX RATIO OF BOVINE EMBRYOS PRODUCED BY FERTILIZATION IN VIVO AND IN VITRO B.R. Yadav, W.A. King, K.P. Xu, L. Picard and K.J. Betteridge.......................356

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DIFFERENCE OF SUPEROWLATION ACCORDING TO DONORS FROM WHICH TRANSFERABLE EMBRYOS WERE OR WERE NOT OBTAINED BY A SINGLE FLUSHING M. Yamamoto, M. Ooe and T. Suzuki....

. . . . . . . . . ...357

A PRELIMINARY STUDY ON THE USE OF A LONG-ACTING GNRH AGONIST PRIOR TO OVARIAN STIMULATION FOR OOCYTE RETRIEVAL IN THE WESTERN LOWLAND GORILLA B. Yee, N.M. Loskutoff, R.C. Cambre, P.P. Call@, R.R. Chacon, T.R. Bowsher and D.C. Kraemer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .358 EFFECT OF EMBEDMENT METHODS ON FREEZING OF MOUSE DEMI-EMBRYOS Y. Zhang AND J. Wang . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .359 PRODUCTION OF MONOZYGOTIC GOAT TWINS BY BISECTION OF FROZEN-THAWED HATCHED BLASTOCYSTS Y. Zhang, J. Wang and J. Xu.........................360 PRODUCTION OF MONOZYGOTIC GOAT TWINS BY TRANSFER OF FROZEN-THAWED DEMI-EMBRYOS Y. Zhang and J. Wang . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .361 IN VITRO DEVELOPMENT OF BOVINE OOCYTES INTO MORULAE AND BLASTOCYSTS *A.I. Younis and B.G. Brackett......................362 EXPRESSION OF PATERNAL MHC CLASS I ANTIGENS RAT PLACENTAE FOLLOWING NATURAL MATING OR EMBRYO TRANSFER X. Zhang, D.T. Armstrong, H.W. Kunz, A. Kanbour-Shakir and T.J. Gill.....................363 IN VITRO MATURATION OF FOLLICULAR OOCYTES FROM THE MOUSE AND DAIRY GOAT Z. Hao, J. Wang and J. Qian.........................364 ULTRARAPIDLY FREEZING OF FOLLICULAR OOCYTES IN MICE 2. Hao, J. Wang and J. Qian.........................365 ISOLATION OF AN OVINE PREGNANCY SPECIFIC PROTEIN A.P. Zoli, J.F. Beckers and F. Ectors...............366 AUTHOR INDEX . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .367

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1990

ACROSOME REACTION AND CONCENTRATION OF PROSTAGLANDIN E2 IN SEMEN OF RAMS TREATED WITH FLUNIXIN MEGLUMINE (BANAMINE) L. Archbald, R.R. Gronwall, E.L. Pritchard and T. Tran . . . . ..a..................................

373

SONOGRAPHIC IMAGING OF THE BOVINE FETUS W. Kahn . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .385 EFFECTS OF SUCKLING MODE ON ENDOCRINE CONTROL OF REPRODUCTIVE ACTIVITY RESUMPTION IN TEXEL EWES LAMBING IN JULY OR NOVEMBER S.N.M. Mandiki, J.L. Bister and R. Paquay........... RAPID MILK PROGESTERONE ASSAY AS A TOOL FOR THE SELECTION OF POTENTIAL DONOR COWS PRIOR TO SUPEROVULATION A. Herrler, F. Elsaesser and H. Niemann.............415 PRELIMANRY CHARACTERIZATION OF PYRUVATE UPTAKE BY ONE-CELL OVINE EMBRYOS J.E. Butler and J.E. Williams.......................423 MATURATION, FERTILIZATION AND DEVELOPMENT OF BOVINE OOCYTES IN VITRO USING TCM199 AND A SIMPLE DEFINED MEDIUM WITH CO-CULTURE C.I. Kim, J.E. Ellington and R.H. Foote.............433 THE EFFECT OF TREATMENT WITH ESTRADIOL VALERATE ON ENDOCRINE CHANGES AND OVARIAN FOLLICLE POPULATIONS IN DAIRY COWS R. Rajamahendran and J.S. Walton....................441 SUPEROVULATORY RESPONSE OF RIVER BUFFALO (Bubalus bubalis) C. Karaivanov, D. Kacheva, M. Petrov, K. Vlahov and E. E. Sapundjiev......................453 THE EFFECT OF POSTBREEDING UTERINE LAVAGE ON PREGNANCY RATES IN MARES S.P. Brinsko, D.D. Varner, T.L. Blanchard and S.A. Meyers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ..465 (continued)

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THERIOGENOLOGY IN VITRO FERTILIZATION AND CLEAVAGE OF BOVINE OOCYTES MATURED IN MEDIUM SUPPLEMENTED WITH ESTROUS COW SERUM K. Schellander, F. Fuhrer, B.G. Brackett, H. Korb and W. Schleger . . . . . . . . . . . . . . . . . . . . . . . . . . . . .477 FERTILIZATION AND EMBRYO LOSS IN BOOROOLA MERINO X SOUTH AUSTRALIAN MERINO EWES: EFFECT OF THE F GENE D.O. Kleeman, S.K. Walker, J.R.W. Walkley, D.H. Smith, R.J. Grimson and R.F. Seamark........... ENCAPSULATION OF PORCINE SPERMATOZOA IN POLY-LYSINE MICROSPHERES K.L. Esbenshade and R.L. Nebel .~.~~.........1..~.~.. 499 INFLUENCE OF NALOXONE AND YOHIMBINE ADMINISTRATION ON PULSATILE LH SECRETION IN LUTEAL PHASE BEEF COWS H.M. Schoenemann, M.W. Richards, S. Sangiah and R.P. Wettemann . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .509 SUPEROVULATION OF BEEF HEIFERS WITH FOLLTROPIN: A NEW FSH PREPARATION CONTAINING REDUCED LH ACTIVITY A. Gonzalez, J.G. Lussier, T.D. Carruthers, B.D. Murphy and R.J. Mapletoft............,..........519 PRENATAL DIAGNOSIS OF SEX IN BOVINE FETUSES BY AMNIOCENTESIS S.P. Leibo and W.F. Rail .*..**......................

531

IN VITRO EXPOSURE OF PREIMPLANTATION PORCINE EMBRYOS TO PORCINE PARVOVIRUS D.P. Bane, J.E. James, C.M. Gradil and T.W. Molitor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .553 SEQUENTIAL UTERINE HORN VERSUS SIMULTANEOUS TOTAL UTERINE FLUSH TO RECOVER BOVINE EMBRYOS NONSURGICALLY J.H. Hay, D.A. Phelps, D.R. Hanks and W.D. Foote . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ..563 EFFECT OF UTERINE FLUID ON IN VITRO ACROSOME REACTION OF BUFFALO (Bubalis bubalis) SPERMATOZOA K.S. Sidhu, A. Bassi, N. Singh and S.S. Guraya . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .569

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Volume

33, Number

3, March 1990

COMMUNICATION BETWEEN THE CORPUS CAVERNOSUM PENIS AND THE CORPUS SPONGIOSUM PENIS IN A BULL DIAGNOSED BY MODIFIED CONTRAST CAVERNOSOGRAPHY: A CASE REPORT R.O. Gilbert and S.S. van den Berg..................577 ONTOGENY OF THE GONADAL RECEPTOR FOR LUTEINIZING HORMONE IN THE PIG A.J. Ziecik, K.L. Esbenshade, H.J. Howard and J.H. Britt . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .583 EFFECTS OF CO-CULTURE, MEDIUM COMPONENTS AND GAS PHASE ON IN VITRO CULTURE OF IN VITRO MATURED AND IN VITRO FERTILIZED BOVINE EMBRYOS H. Nakao and N. Nakatsuji . . . . . . . . . . . . . . . . . . . . . . . . . . .591 THE EFFECTS OF XYLAZINE AND APLHA-ADRENORECEPTOR ANTAGONISTS ON BOVINE UTERINE CONTRACTILITY IN VITRO J.C.H. Ko, W.H. Hsu and L.E. Evans..................601 PARTURITION IN BEEF COWS FOLLOWING ADMINISTRATION OF PORCINE RELAXIN AT TEN DAYS PREPARTUM R.W. Caldwell, J.C. Whittier, M.F. Smith, R.E. Morrow and R.V. Anthony........................613 EFFECTS OF EQUILIBRATION TIME, PRECOOLING AND DEVELOPMENTAL STAGE ON THE SURVIVAL OF MOUSE EMBRYOS CRYOPRESERVED BY VITRIFICATION C.A. Valdez, 0. Abas Mazni, Y. Takahashi, M. Hishinuma and H. Kanagawa........................627 SITE OF ACTION FOR ENDOTOXIN-INDUCED CORTISOL RELEASE IN THE SUPPRESSION OF PREOVULATORY LUTEINIZING HORMONE SURGES A.T. Peter, J.E. Simon, C.W. Luker and W.T.K. BOSU . . . . . . . . . . .. . . . . . . .. . . . . . . . . . . . . . . . . .637 THE EFFECT OF ECHERICHIA COLI ENDOTOXIN ON LUTEAL FUNCTION IN HOLSTEIN HEIFERS R.O. Gilbert, W.T.K. Bosu and A.T. Peter.. . . ...... ..64 5 EFFECTS OF FEEDING ENDOPHYTE-INFECTED TALL FESCUE SEED ON REPRODUCTIVE PERFORMANCE IN MALE CD-l MICE BY COMPETITIVE BREEDING P.M. Zavos, M.R. Siegel, R.J. Grove, R.M. Hemken and D.R. Varney...............

. . ...... ..65 3

(continued)

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THERIOGENOLOGY RESPONSE OF PREPUBERTAL Bos taurus and Bos indicus x Bos taurus HEIFERS TO MELENGESTROL ACETATE WITH OR WITHOUT GONADOTROPIN-RELEASING HORMONE D.J. Patterson, L.R. Corah and J.R. Brethour . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . .661 CELL NUMBER AND INCIDENCE OF CHROMOSOMAL ANOMALIES IN BOVINE BLASTOCYSTS FERTILIZED IN VITRO FOLLOWED BY CULTURE IN VITRO OR IN VIVO IN RABBIT OVIDUCTS S. Iwasaki and T. Nakahara . . . . . . . . . . . . . . . . . . . . . . . . . .669 WILL THE FIRST DOMINANT FOLLICLE OF THE ESTROUS CYCLE OF HEIFERS OVULATE FOLLOWING LUTEOLYSIS ON DAY 71 J.D. Savio, M.P. Boland, N. Hynes, M.R. Mattaicci and J.F. Roche.......................677 PISUM SATIWM AGGLUTININ AS AN ACROSOMAL STAIN OF PORCINE AND CAPRINE SPERM T. Berger . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .689 EARLY PREGNANCY DIAGNOSIS IN CATTLE BY MEANS OF LINEAR-ARRAY REAL-TIME ULTRASOUND SCANNING OF THE UTERUS AND A QUALITATIVE AND QUANTITATIVE MILK PROGESTERONE TEST M.C. Pieterse, 0. Szenci, A.H. Willemse, C.S.A. Bajcsy, S.J. Dieleman and M.A.M. Taverne . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .697 LIMITATIONS OF OVIDUCTAL TRANSFERS IN SWINE D.M. Broermann, S. Xie, K.P. Nephew and W.F. Pope . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .709 LYSOZYME, ALKALINE PHOSPHATASE AND NEUTROPHILS IN UTERINE SECRETIONS OF MARES WITH DIFFERING RESISTANCE TO ENDOMETRITIS T. Katila, T.F. Lock, W.E. Hoffman and A.R. Smith . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .723 EFFECT OF DIALYSIS OR CENTRIFUGATION OF POST-THAW MOTILITY AND FERTILITY OF SANTA GERTRUDIS BULL SEMEN COLLECTED BY ELECTROEJACULATION A. Tibary, E.F. Graham, A. Asri, R. Boukhliq and R. Deyo . . . . . . . . . . . . . . . . . . . . . . . . . . . . .733 THE EFFECT OF SUCROSE AND TREHALOSE ON VIABILITY OF ONE- AND TWO-CELL RABBIT EMBRYOS Z. Smorag, Y. Heyman, V. Garnier and B. Gajda . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .741

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33, Number

4, April 1990

EFFECT OF TIME AND TEMPERATURE OF INCUBATION OF HEPARINIZED CAPRINE BLOOD ON CONCENTRATIONS OF PROGESTERONE DETECTED IN PLASMA H. Navarro, L. Zarco, A. Ducoing, G. Flores and J. Valencia . . . . . . . . . . . . . . . . . . . . . . . . . . .749 TEMPORARY INHIBITION OF MEIOSIS RESUMPTION IN VITRO BY ADENYLATE CYCLASE STIMULATION IN IMMATURE BOVINE OOCYTES M.A. Sirard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .757 OVARIAN DEVELOPMENT IN MEISHAN PIGS T. Miyano, J. Akamatsu, S. Kato, I. Nanjo and S. Kanda . . . . . . . . . . . . . . . . . . ..*.......... 769 THE SURVIVAL OF WHOLE AND BISSECTED RABBIT MORULAE AFTER CRYOPRESERVATION BY THE VITRIFICATION METHOD K. Kobayashi, H. Nagashima, H. Yamakawa, Y. Kate and S. Ogawa . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .777 SHORT-TERM HYPOTONIC TREATMENT FOR CHROMOSOME PREPARATION OF INTACT AND ZONA-PENETRATED MOUSE EMBRYOS M. Yoshizawa, S. Nakamoto, Y. Ysunoda and T. Muramatsu . . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .789 VARIATION IN GLYCOGEN AND MUCINS IN THE EQUINE UTERUS RELATED TO PHYSIOLOGIC AND PATHOLOGIC CONDITIONS K.P. Freeman, J.F. Roszel, S.H. Slusher and M. Castro . . . . . . . . . . . . . ..*.......................

799

INDOMETHACIN INFLUENCES ARGININE VASOTOCIN-INDUCED PARTURITION AND OVIPOSITION IN LIZARDS (Sceloporus jarrovi and Sceloporus undulatus) L.J. Guillette, Jr., A. Pfrimmer Hensley, J.M. Matter and P.H. Jaffe *.*.*......*.*......**.**.

809

THE EFFICIENCY OF PRODUCTION, CENTRIFUGATION, MICROINJECTION AND TRANSFER OF ONE- AND TWO-CELL BOVINE OVA IN A GENE TRANSFER PROGRAM T.G. McEvoy and J.M. Sreenan........................819 (continued)

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THERIOGENOLOGY AND COMPARISON OF THE EFFECTS OF PGF2 BROMOCRYPTINE IN PREGNANT BEAGLE BITCHES J.J. Witchel, M.D. Whitacre, D.J. Yates and S.D. Van Camp . . . . . . . . . . . . . . . . . . . . . . . . ..*........ 829 INFLUENCE OF GESTATION ON JUGLAR PLASMA THYROXINE LEVELS IN MURRAH BUFFALO AND KARAN SWISS COWS N.N. Hung and B.S. Prakash . . . . . . . . . . .. . . . . . . . . . . .. . .837 EFFECT OF SIDE OF INSEMINATION ON TRANSUTERINE TRANSPORT OF SPERMATOZOA IN SUPEROVULATED DAIRY CATTLE F. Lopez-Glatius and J. Camon_Urgel.................843 BACTERIAL CONTAMINATION OF THE UTERUS AFTER PARTURITION AND ITS EFFECT ON THE REPRODUCTIVE PERFORMANCE OF COWS ON LARGE-SCALE DAIRY FARMS T. Takacs, I. Gathy, 2. Machaty and E. Bajmocy......851 ULTRASONIC EXAMINATION AND MEASUREMENT OF RAM TESTICLES R.E. Cartee, P.F. Rumph, S. Abuzaid and R. Carson . . . . . . . . . . . . . . . . . . . . . . . ..*............. 867 THE EFFECT OF PROSTAGLANDIN ADMINISTRATION TO HOLSTEIN-FRIESIAN COWS AT DAY 26 POSTPARTUM ON CLINICAL FINDINGS, AND HISTOLOGICAL AND BACTERIOLOGICAL RESULTS OF ENDOMETRIAL BIOPSIES AT DAY 40 B.N. Bonnett, W.G. Etherington, S.W. Martin and W.H. Johnson . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . .. . . .877 ENDOCRINE RESPONSES IN THE LLAMA TO COPULATION P.W. Bravo, M.E. Fowler, G.H. Stabenfeldt and B. Lasley . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .891 A METHOD FOR CULTIVATING MORPHOLOGICALLY UNDIFFERENTIATED EMBRYONIC STEM CELLS FROM PORCINE BLASTOCYSTS R.M. Strojek, M.A. Reed, T.L. Hoover, and T.E. Wagner . . . .. . . . . . . . .. . . . . . . . . . . .. . .. . . . . . . . .901 MONITORING OVARIAN CYCLICITY IN POSTPARTUM MURRAH BUFFALO THROUGH MILK PROGESTERONE ENZYMEIMMUNOASSAY Y.P. Sharma and M.L. Kaker . . . . . . . . . . . . . . . . . . . . . . . . . .915 ELEVATION OF MORPHOLOGICAL ABNORMALITIES OF SPERMATOZOA IN THE SEMEN OF ZEBU BULLS CONSEQUENT TO Trypanosoma vivax AND Trypanosoma congolense INFECTIONS V.O. Sekoni, D.I. Saror, C.O. Njoko and J. Kumi-Diaka . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .925

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THERIOGENOLOGY Volume

33, Number

5,

May 1990

A SIMPLE TECHNIQUE THAT MAY IMPROVE WORK IN PROGRESS: THE RATE OF EMBRYO RECOVERY ON UTERINE FLUSHING IN MARES K. Hinrichs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .937 SYNCHRONIZATION OF ESTRUS AFTER TREATMENT WITH LUPROSITOL IN BEEF COWS AND IN BEEF AND DAIRY HEIFERS N.I. Plata, J.C. Spitzer, C.E. Thompson, D.M. Hendricks, M.P. Reid and T.J. Newby............ CLONED OVARIAN CELLS FROM SHEEP: CHOLESTEROL DEPENDENCY OF PROGESTERONE SYNTHESIS M.J.D. Thatcher, B. Charreau, A. Paris, M. Terqui, A. Paraf and W.W. Thatcher . . . . . . . . . . . . . . . . . . . . . . . . . .953 FACTORS INFLUENCING LAMB SURVIAL IN A HIGH FECUNDITY BOOROOLA MERINO X SOUTH AUSTRALIAN MERINO FLOCK D.O. Kleeman, S.K. Walker, J.R.W. Walkley, D.H. Smith, R.W. Ponzoni and R.F. Seamark........... THE STRUCTURE OF THE CERVICAL CANAL OF THE EWE G.W. Halbert, H. Dobson, J.S. Walton and B.C. Buckrell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .977 A TECHNIQUE FOR TRANSCERVICAL INTRAUTERINE INSEMINATION OF EWES G.W. Halbert, H. Dobson, J.S. Walton and B.C. Buckrell . . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .993 ABSORPTION OF (Escherichia coli) ENDOTOXIN (LIPOPOLYSACCHARIDE) FROM THE UTERI OF POSTPARTUM DAIRY COWS A.T. Peter, W.T.K. Bosu and R-0, Gilbert...........1011 INHIBITION OF IMPLANTATION IN VITRO OF FROZEN-THAWED MOUSE EMBRYOS BY THE SIGNAL MOLECULE DIADENOSINE TETRAPHOSPHATE P.J. Chan, I. Henig, S.G. Prough and D.R. Tredway . . . . . ..*...............................

1015

MIGRATION OF SPERMATOZOA IN THE OVIDUCT OF HENS FOLLOWING INTRAVAGINAL, INTRAMAGNAL AND INTRAABDOMINAL INSEMINATION Jean-Pierre Brillard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1021 COMPARISON OF RAM SPERM INTERACTION WITH BOVINE CERVICAL MUCUS J.R. Mole and J.A. Fitzgerald......................1031 (continued)

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THERIOGENOLOGY EFFECTS OF BLOOD MONOCYTES AND LYMPHOCYTES ON PROGESTERONE SECRETION BY GRANULOSA CELLS IN THE PIG R. Maier and B.P. Chew . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1045 THE EFFECTS OF METHYLTESTOSTERONE ON REPRODUCTIVE FUNCTION IN MALE GREYHOUNDS J.L. Freshman, P.N. Olson, R.P. Amann, E.D. Carlson, D.C. Twedt and R.A. Bowen............ ON THE PATHOGENESIS OF ABORTION IN ACUTE NITRATE TOXICOSIS OF PREGNANT DAIRY COWS A.Th. van 't Klooster, M.A.M. Taverne, A. Malestein and E.M. Akkersdijk...................lO75 PROGESTERONE DETERMINATIONS AND CLINICAL EXAMINATIONS OF REPRODUCTIVE ORGANS IN PUREBRED AND CROSSBRED FEMALE ZEBU CATTLE M. Garcia and L-E. Edqvist.........................lO91 MILK PROGESTERONE PROFILES IN CROSSBRED BROWN SWISS X NELLORE CATTLE CATTLE FOLLOWING NATURAL SERVICE M. Garcia . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1105 THE EFFECT OF INFECTIOUS BOVINE RHINOTRACHEITIS VACCINE ON REPRODUCTIVE EFFICIENCY IN CATTLE VACCINATED DURING ESTRUS B.C. Chlang, P.C. Smith, K.E. Nusbaum and D.A. Stringfellow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1113 THE EFFECT OF POSTPARTUM UTERINE LAVAGE ON FOAL HEAT PREGNANCY RATE P.M. McCue and J.P. Hughes.........................1121 MULTIPLE OWLATION AND EMBRYO TRANSFER IN INDIAN BUFFALO (Bubalus bubalis) A.K. Misra, B.V. Joshi, P.L. Abrawala, R. Kasiraj, S. Sivaiah, N.S. Rangareddi and M.U. Siddiqui......ll31 INCREASING LAMBING PERFORMANCE OF EWES BY ACTIVE IMMUNIZATION WITH POLYANDROALBUMIN R.K. Daroliya, S.K. Verma and R.K. Chandolia.......ll43 THE EFFECT OF PMSG-PRIMING ON SUBSEQUENT SUPEROVULATORY RESPONSE IN DAIRY COWS J. Petr, J. Mika and F. Jilek......................ll51

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THERIOGENOLOGY Number 6, June 1990 EFFECTS OF PHOTOPERIOD ON REPRODUCTION IN TURKEY BREEDER HENS B.T. Slaugh, N.P. Johnston and R.K. Bramwell......

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PARTHENOGENETIC ACTIVATION OF MOUSE AND PIG OOCYTES MATURED IN VITRO B.A. Didion, M.J. Martin and C.L. Markert..........ll65 DIMETHYL SULFOXIDE INTRAUTERINE THERAPY IN THE MARE: EFFECT UPON UTERINE-DERIVED NEUTROPHIL FUNCTION W.B. Ley, D.L. Ochs, E.S. Metcalf, H. Pyle, B.J. Purswell and J.M. Bowen....................... VIABILITY OF ONE-CELL BOVINE EMBRYOS CULTURED IN SYNTHETIC OVIDUCT FLUID MEDIUM K.J. McLaughlin, D.M. McLean, G. Stevens, R.A. Ashman, P.A. Lewis, B.D. Bartsch and R.F. Seamark . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1191 MANUAL STIMULATION COLLECTION OF SEMEN FROM STALLIONS: TRAINING TIME, SEXUAL BEHAVIOR AND SEMEN S.M. McDonnell and C.C. Love.......................1201 FLOW CYTOMETRIC EVALUATION OF CRYOPRESERVED BOVINE SPERMATOZOA PROCESSED USING A NEW ANTIBIOTIC COMBINATION S.A. Ericsson, D.L. Garner, L.A. Johnson, D. Redelman and K. Ahmad . . . . .. . . . . . . . . . . . . . . . . . . . . .1211 BEHAVIOUR OF LIVING PRIMORDIAL GERM CELLS OF LIVESTOCK IN VITRO F. Leichthammer, E. Baunack and G. Brem............1221 FIELD EVALUATION OF A TECHNIQUE FOR TRANSCERVICAL INTRAUTERINE INSEMINATION OF EWES G.W. Halbert, H. Dobson, J.S. Walton, P. Sharpe and B.C. Buckrell........................l231 EFFECT OF EMBRYO QUALITY AND STAGE OF DEVELOPMENT ON THE SURVIVAL OF ZONA PELLUCIDA-FREE CATTLE DEMI-EMBRYOS T.G. McEvoy and J.M. Sreenan.......................l245 (continued)

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THERIOGENOLOGY NORMAL OFFSPRING PRODUCED EMBRYOS GROWN FROM TWO TO CHEMICALLY-DEFINED CULTURE S.A. Schini and A.D.

AFTER TRANSFER OF HAMSTER EIGHT CELLS IN A MEDIUM Bavister......................l255

EFFECT OF ASPIRATION OF FOLLICULAR FLUID ON SUBSEQUENT LUTEAL FUNCTION IN THE MARE E.D. Watson and P.L. Sertich.......................l263 RELATIONSHIP BETWEEN ULTRASONIC ASSESSMENT OF THE CORPUS LUTEUM AND PLASMA PROGESTERONE CONCENTRATIONS IN HEIFERS J.P. Kastelic, D.R. Bergfelt and O.J. Ginther......l269 THE EFFECT OF PROGESTAGEN TYPE, PMSG DOSAGE AND TIME OF RAM INTRODUCTION ON REPRODUCTIVE PERFORMANCE IN EWE LAMBS J.V. O'Doherty and T.F. Crosby.....................1279 INFLUENCE OF PARITY AND LITTER SIZE ON ESTROUS CYCLES AND PROGESTERONE PATTERNS IN SOWS R.C. Tubbs, D.K. Hardin, N.M. Cox and A.W. Grace . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . . . . .1287 THE APPLICATION OF PROGESTERONE-CONTAINING CIDR DEVICES TO SUPEROVULATED EWES J.G.E. Thompson, A.C. Simpson, R.W. James and H.R. Tervit.........................l297 THE EFFECT OF A POTENTIAL GnRH AGONIST ON GONADAL AND SEXUAL ACTIVITY IN THE HORSE S.M. Montavon, P.F. Daels, J. Rivier, J.P. Hughes, G.H. Stabenfelt and B.L. Lasley . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. .1305 THE RESPONSE OF EWES FROM THREE LOCAL GREEK BREEDS TO A LONG-DAY SKELETON PHOTOPERIOD DURING THE WINTER D. Amir and A. Zaralis . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1323

DESCRIPTIVE EPIDEMIOLOGY AND TREATMENT OF POSTPARTUM ANESTRUS IN DAIRY BUFFALO UNDER SMALL FARM CONDITIONS S. Nasir Hussain Shah, A.H. Willemse and D.F.M. Van de Weil . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1333 INDEX

(Volume 33) ...............................

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SUBJECT INDEX

(Volume 33) ...............................

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(Volume 33) ....................................

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AUTHORS

CONTENTS

JUNE199OVOL.33NO.6

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