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Continuing Medical Education examination
Reviews and feature articles
Continuing Medical Education examination
The role of activation-induced cytidine deaminase in antibody diversification, immunodeficiency, and B-cell malignancies Instructions for category 1 Continuing Medical Education credit The American Academy of Allergy, Asthma and Immunology is accredited as a provider of Continuing Medical Education (CME) by the Accreditation Council for Continuing Medical Education. Test ID no.: mai0044 Contact hours: 1.0 Expiration date: September 30, 2005 Category 1 credit can be earned by reading the text material and taking this CME examination online. For complete instructions, visit the Journal’s Web site at www.mosby.com/jaci.
Learning objectives: ‘‘The role of activation-induced cytidine deaminase in antibody diversification, immunodeficiency, and B-cell malignancies’’ 1. To understand the role of activation-induced cytidine deaminase (AID) in somatic hypermutation and class switch recombination. 2. To understand why somatic hypermutation and class switch recombination are required to generate a diverse and useful antibody response to infectious agents. 3. To understand why mutations in the AID gene cause immunodeficiency and predispose to B-cell malignancies.
CME items Question 1. Which of the following processes occurs in the B cells in the dark zone of the germinal centers? A. V(D)J rearrangement B. somatic hypermutation and class switch recombination C. positive selection of B-cell clones that have high affinity to a specific antigen D. soluble antibody secretion Question 2. Which of the following features is unique for class switch recombination but not for somatic hypermutation? A. Transcription is required. B. Point mutations are often found in the DNA sequence involved. C. Activation-induced cytidine deaminase (AID) plays a key role in this process. D. There is recombination between the donor and recipient switch regions, with the looping-out of a piece of DNA fragment containing some of the constant region genes.
736
October 2004
Question 3. The C-terminal deletion mutant of AID lost the ability to carry out class switch recombination but retained the ability to carry out somatic hypermutation. Which of the following hypotheses does this piece of information support? A. One or more cofactors are probably needed to target AID to the switch region in order to initiate class switch recombination. B. AID is a cytidine deaminase like APOBEC-1; RNA is the only possible substrate of AID. C. AID is a secreted protein. D. AID causes mutations in the V region but not in SRs. Question 4. Which of the following lymphomas is considered to have derived from the plasma-cells stage of B-cell development? A. follicular lymphoma B. Burkitt’s lymphoma C. multiple myeloma D. diffuse large B-cell lymphoma
J ALLERGY CLIN IMMUNOL
Continuing Medical Education examination
The role of activation-induced cytidine deaminase in antibody diversification, immunodeficiency, and B-cell malignancies Instructions for category 1 Continuing Medical Education credit The American Academy of Allergy, Asthma and Immunology is accredited as a provider of Continuing Medical Education (CME) by the Accreditation Council for Continuing Medical Education. Test ID no.: mai0044 Contact hours: 1.0 Expiration date: September 30, 2005 Category 1 credit can be earned by reading the text material and taking this CME examination online. For complete instructions, visit the Journal’s Web site at www.mosby.com/jaci.
Learning objectives: ‘‘The role of activation-induced cytidine deaminase in antibody diversification, immunodeficiency, and B-cell malignancies’’ 1. To understand the role of activation-induced cytidine deaminase (AID) in somatic hypermutation and class switch recombination. 2. To understand why somatic hypermutation and class switch recombination are required to generate a diverse and useful antibody response to infectious agents. 3. To understand why mutations in the AID gene cause immunodeficiency and predispose to B-cell malignancies.
CME items Question 1. Which of the following processes occurs in the B cells in the dark zone of the germinal centers? A. V(D)J rearrangement B. somatic hypermutation and class switch recombination C. positive selection of B-cell clones that have high affinity to a specific antigen D. soluble antibody secretion Question 2. Which of the following features is unique for class switch recombination but not for somatic hypermutation? A. Transcription is required. B. Point mutations are often found in the DNA sequence involved. C. Activation-induced cytidine deaminase (AID) plays a key role in this process. D. There is recombination between the donor and recipient switch regions, with the looping-out of a piece of DNA fragment containing some of the constant region genes.
736
October 2004
Question 3. The C-terminal deletion mutant of AID lost the ability to carry out class switch recombination but retained the ability to carry out somatic hypermutation. Which of the following hypotheses does this piece of information support? A. One or more cofactors are probably needed to target AID to the switch region in order to initiate class switch recombination. B. AID is a cytidine deaminase like APOBEC-1; RNA is the only possible substrate of AID. C. AID is a secreted protein. D. AID causes mutations in the V region but not in SRs. Question 4. Which of the following lymphomas is considered to have derived from the plasma-cells stage of B-cell development? A. follicular lymphoma B. Burkitt’s lymphoma C. multiple myeloma D. diffuse large B-cell lymphoma
J ALLERGY CLIN IMMUNOL