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Copper, selenium, zinc and chromium status in occupational manganese exposed workers
Abstracts / Toxicology Letters 238S (2015) S56–S383
organization and preservation of the myelin envelope. The purpose of this study is to evaluate the blood levels of folate and B12 in lead exposed patients and compare them with control subjects. Methods: 1179 lead exposed and 1200 nonexposed workers were included in this study. Measurements of blood lead levels were determined by Ankara Occupational Diseases Hospital Toxicology Laboratory using Agilent ICP-MS. Folate and Vitamin B12 levels were determined by Architect I˙ 2000 SR. Results: The mean blood lead levels of lead exposed group and control subjects were 26.05 g/dl (16–50) and 2.5 g/dl (0.30–8.50) respectively. The mean blood vitamin B12 level of lead exposed group was significantly lower than non-exposed group (210 pg/ml vs. 270 pg/ml), p < 0.05. Also the mean blood folate level of lead exposed group was significantly lower than non-exposed (6.22 ng/ml vs. 6.85 ng/ml), p < 0.001. Conclusions: The results showed, folate and Vitamin B12 levels were significantly lower in lead exposed group. This suggests lead alters folate and vitamin B12 metabolism and may be some of its neurologic effects are arise from this pathways. Prospective studies must be performed to establish the link.
Results: The mean blood Mn level of exposed group and nonexposed group were 21.5 g/L (18.7–66) and 5.8 g/L (4.8–7.9) respectively (p < 0.001). The mean blood copper level of Mn exposed group was significantly higher than non-exposed group, 102 ± 19.16 g/dl vs. 94.75 ± 16.64 g/dl (p < 0.05). The blood mean levels of selenium, zinc and chromium were not significantly different between Mn exposed group and non-exposed group. Conclusions: The results show that copper levels were significantly higher in manganese exposed group. This suggests that toxic metals can deteriorate the levels of essential minerals. The indifference with other metals may be due to insufficient number of patients. Prospective studies must be performed with larger study groups to establish the link and causality. http://dx.doi.org/10.1016/j.toxlet.2015.08.460
P05-022 The trace element pattern in occupational lead exposed workers
http://dx.doi.org/10.1016/j.toxlet.2015.08.459
M.E. Alagüney 1 , C. Bal 2 , A. Hocaoglu 3,∗ , M. Büyüks¸ekerci 4 , L. Tutkun 5 , O.H. Yılmaz 6
P05-021 Copper, selenium, zinc and chromium status in occupational manganese exposed workers
1
M. Büyüks¸ekerci 1 , C. Bal 2 , A. Hocaoglu 3,∗ , M.E. Alagüney 4 , O.H. Yılmaz 5 , E. Tutkun 6 1 Ankara Occupational Disease Hospital, Pharmacology, Ankara, Turkey 2 Ankara Occupational Disease Hospital, Biochemistry, Ankara, Turkey 3 Ankara University Institute of Forensic Science, Ankara, Turkey 4 Hacettepe University, Internal Medicine, Ankara, Turkey 5 Yıldırım Beyazıt University, Public Health, Ankara, Turkey 6 Ankara Occupational Disease Hospital, Toxicology, Ankara, Turkey
Question: Manganese (Mn) is a naturally occurring element found in many types of rocks or soil and forms compounds with oxygen, sulfur and chlorine. Mn is utilized widely in industry; mostly in steel production to improve hardness, stiffness and strength. It is also used in many products including fireworks, drycell batteries, paints, fertilizer, cosmetics, and as medical imaging agent. It is an essential element and is required for normal development and body function in humans. Mn either regulates/binds to a variety of enzymes such as superoxide dismutase, arginase, pyruvate carboxylase. Occupational exposure to Mn occurs via inhalation of metal fume and dust which leads to adverse health effects. Exposure to high levels of airborne Mn in industries including welding, mining, Mn alloy and dry battery production can cause manganism; a neurodegenerative extrapyramidal syndrome by psychiatric symptoms. Trace elements have been contributed genuine insights into developmental neurotoxicity and they serve as paradigms for such adverse effects. In this study we aimed to investigate the effect of occupationally exposure of Mn on the content of trace elements such as copper (Cu), selenium (Se), zinc (Zn) and chromium (Cr). Methods: 125 manganese exposed and 155 nonexposed workers were included in this study. Measurements of blood manganese, copper, zinc, selenium and chromium levels were determined by Ankara Occupational Diseases Hospital Toxicology Laboratory using Agilent ICP-MS.
S147
Hacettepe University, Internal Medicine, Ankara, Turkey Ankara Occupational Disease Hospital, Biochemistry, Ankara, Turkey 3 Ankara University Institute of Forensic Science, Ankara, Turkey 4 Ankara Occupational Disease, Pharmacology, Ankara, Turkey 5 Hacettepe University, Ankara, Turkey 6 Yıldırım Beyazıt University, Public Health, Ankara, Turkey 2
Question: Lead (Pb) have long been used by man and today industrial demand for Pb is still rising. Lead interacts with some essential elements including copper, zinc, selenium and chromium. Copper binds to metallothionein-like proteins and is involved in caeruloplasmin which is responsible for oxidation of ferrous to ferric iron and catalyses the transport of iron to transferrin. Lead binds either to caeruloplasmin or transferrin and may disrupt iron metabolism. Zinc (Zn) reduces the oxidative stress induced by Pb as it is a component of superoxide dismutase and Zn also competes with lead for similar binding sites. Lead–selenium interaction is also observed. Selenium plays important role in antioxidant system as a cofactor of glutathione peroxidase which protects cellular components such as DNA, RNA and proteins from oxidative damage. The formation of inactive selenium–lead complexes reduces the availability of free lead ions in body. The aim of this study is to determine the effect of occupational exposure to lead on blood levels of copper, zinc, selenium and chromium. Methods: 1179 lead exposed and 1200 nonexposed workers were included in this study. Measurements of blood lead, copper, zinc, selenium and chromium levels were determined by Ankara Occupational Diseases Hospital Toxicology Laboratory using Agilent ICP-MS. Results: The mean blood Pb level of lead exposed group and control subjects were 26.05 g/dl (16–50) and 2.5 g/dl (0.30–8.50) respectively. The mean blood copper level of lead exposed group was significantly lower than non-exposed control group, 91 g/dl (71–134) vs. 97 g/dl (55–197), p < 0.005. Likewise blood mean zinc level of lead exposed group was significantly lower than in non-exposed group, 93 g/l (60–200) vs. 97 g/l (54–171), p < 0.01. However mean blood levels of selenium and chromium of lead exposed group and non-exposed group were not significantly different. Conclusions: The results show, copper and zinc levels were significantly lower in lead exposed group. This suggests that lead
organization and preservation of the myelin envelope. The purpose of this study is to evaluate the blood levels of folate and B12 in lead exposed patients and compare them with control subjects. Methods: 1179 lead exposed and 1200 nonexposed workers were included in this study. Measurements of blood lead levels were determined by Ankara Occupational Diseases Hospital Toxicology Laboratory using Agilent ICP-MS. Folate and Vitamin B12 levels were determined by Architect I˙ 2000 SR. Results: The mean blood lead levels of lead exposed group and control subjects were 26.05 g/dl (16–50) and 2.5 g/dl (0.30–8.50) respectively. The mean blood vitamin B12 level of lead exposed group was significantly lower than non-exposed group (210 pg/ml vs. 270 pg/ml), p < 0.05. Also the mean blood folate level of lead exposed group was significantly lower than non-exposed (6.22 ng/ml vs. 6.85 ng/ml), p < 0.001. Conclusions: The results showed, folate and Vitamin B12 levels were significantly lower in lead exposed group. This suggests lead alters folate and vitamin B12 metabolism and may be some of its neurologic effects are arise from this pathways. Prospective studies must be performed to establish the link.
Results: The mean blood Mn level of exposed group and nonexposed group were 21.5 g/L (18.7–66) and 5.8 g/L (4.8–7.9) respectively (p < 0.001). The mean blood copper level of Mn exposed group was significantly higher than non-exposed group, 102 ± 19.16 g/dl vs. 94.75 ± 16.64 g/dl (p < 0.05). The blood mean levels of selenium, zinc and chromium were not significantly different between Mn exposed group and non-exposed group. Conclusions: The results show that copper levels were significantly higher in manganese exposed group. This suggests that toxic metals can deteriorate the levels of essential minerals. The indifference with other metals may be due to insufficient number of patients. Prospective studies must be performed with larger study groups to establish the link and causality. http://dx.doi.org/10.1016/j.toxlet.2015.08.460
P05-022 The trace element pattern in occupational lead exposed workers
http://dx.doi.org/10.1016/j.toxlet.2015.08.459
M.E. Alagüney 1 , C. Bal 2 , A. Hocaoglu 3,∗ , M. Büyüks¸ekerci 4 , L. Tutkun 5 , O.H. Yılmaz 6
P05-021 Copper, selenium, zinc and chromium status in occupational manganese exposed workers
1
M. Büyüks¸ekerci 1 , C. Bal 2 , A. Hocaoglu 3,∗ , M.E. Alagüney 4 , O.H. Yılmaz 5 , E. Tutkun 6 1 Ankara Occupational Disease Hospital, Pharmacology, Ankara, Turkey 2 Ankara Occupational Disease Hospital, Biochemistry, Ankara, Turkey 3 Ankara University Institute of Forensic Science, Ankara, Turkey 4 Hacettepe University, Internal Medicine, Ankara, Turkey 5 Yıldırım Beyazıt University, Public Health, Ankara, Turkey 6 Ankara Occupational Disease Hospital, Toxicology, Ankara, Turkey
Question: Manganese (Mn) is a naturally occurring element found in many types of rocks or soil and forms compounds with oxygen, sulfur and chlorine. Mn is utilized widely in industry; mostly in steel production to improve hardness, stiffness and strength. It is also used in many products including fireworks, drycell batteries, paints, fertilizer, cosmetics, and as medical imaging agent. It is an essential element and is required for normal development and body function in humans. Mn either regulates/binds to a variety of enzymes such as superoxide dismutase, arginase, pyruvate carboxylase. Occupational exposure to Mn occurs via inhalation of metal fume and dust which leads to adverse health effects. Exposure to high levels of airborne Mn in industries including welding, mining, Mn alloy and dry battery production can cause manganism; a neurodegenerative extrapyramidal syndrome by psychiatric symptoms. Trace elements have been contributed genuine insights into developmental neurotoxicity and they serve as paradigms for such adverse effects. In this study we aimed to investigate the effect of occupationally exposure of Mn on the content of trace elements such as copper (Cu), selenium (Se), zinc (Zn) and chromium (Cr). Methods: 125 manganese exposed and 155 nonexposed workers were included in this study. Measurements of blood manganese, copper, zinc, selenium and chromium levels were determined by Ankara Occupational Diseases Hospital Toxicology Laboratory using Agilent ICP-MS.
S147
Hacettepe University, Internal Medicine, Ankara, Turkey Ankara Occupational Disease Hospital, Biochemistry, Ankara, Turkey 3 Ankara University Institute of Forensic Science, Ankara, Turkey 4 Ankara Occupational Disease, Pharmacology, Ankara, Turkey 5 Hacettepe University, Ankara, Turkey 6 Yıldırım Beyazıt University, Public Health, Ankara, Turkey 2
Question: Lead (Pb) have long been used by man and today industrial demand for Pb is still rising. Lead interacts with some essential elements including copper, zinc, selenium and chromium. Copper binds to metallothionein-like proteins and is involved in caeruloplasmin which is responsible for oxidation of ferrous to ferric iron and catalyses the transport of iron to transferrin. Lead binds either to caeruloplasmin or transferrin and may disrupt iron metabolism. Zinc (Zn) reduces the oxidative stress induced by Pb as it is a component of superoxide dismutase and Zn also competes with lead for similar binding sites. Lead–selenium interaction is also observed. Selenium plays important role in antioxidant system as a cofactor of glutathione peroxidase which protects cellular components such as DNA, RNA and proteins from oxidative damage. The formation of inactive selenium–lead complexes reduces the availability of free lead ions in body. The aim of this study is to determine the effect of occupational exposure to lead on blood levels of copper, zinc, selenium and chromium. Methods: 1179 lead exposed and 1200 nonexposed workers were included in this study. Measurements of blood lead, copper, zinc, selenium and chromium levels were determined by Ankara Occupational Diseases Hospital Toxicology Laboratory using Agilent ICP-MS. Results: The mean blood Pb level of lead exposed group and control subjects were 26.05 g/dl (16–50) and 2.5 g/dl (0.30–8.50) respectively. The mean blood copper level of lead exposed group was significantly lower than non-exposed control group, 91 g/dl (71–134) vs. 97 g/dl (55–197), p < 0.005. Likewise blood mean zinc level of lead exposed group was significantly lower than in non-exposed group, 93 g/l (60–200) vs. 97 g/l (54–171), p < 0.01. However mean blood levels of selenium and chromium of lead exposed group and non-exposed group were not significantly different. Conclusions: The results show, copper and zinc levels were significantly lower in lead exposed group. This suggests that lead