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Coronary microangiography in the guinea pig
91MYOCAROIAL CONTENT OF Darrecott-Cankovic, Rayne Institute, Oedema efter cardiec in the clinical developed interference photographic of the scan. content of interfibrillar the mass of Water content interferometry workin ret 37'C Pn=121 intrafibrillar reperfusion. cardioplegia. the operating in clinicel cannot, its
OEOEMA: A DUANTITATIVE METHOD FOR VISUALISING CHANGES IN DISTRIBUTION AND MYOCARDIAL WATER DURING EXPERIMENTAL AND CLINICAL PROCEDURES. S. Cardiothoracic Research Unit, The H.V. Braimbridge, J. Cheyen. St. Themes' Hospital, London SEI, U.K. of the Left ventricle 1LVI has been shown to occur during reparfusion arrest. Conventional wet end dry weight [W/O) analysis is not feasible We heve situation because of the smell size of tissue semples. a technique whereby a fresh cryostat section is placed under an microscope, en area of Longitudinal muscle fibres is photographed, the negative is passed under e scanning densitometer which records e trace Muscle fibres appear as peaks on the trace, height varying with weter fibres. the Troughs to beseline between muscle fibres, correspond to wetar. An integrator etteched to the densitometer gives a value for any area of tissue scenned. Normal ret LV has e water content of 76%. of rat LV samples ln=131 was assessed by W/O and by microscopic (MI); a coefficient of correletion of 0.67 was obtained. In isolated heerts W/D revealed 62% water following 35 minutes cardioplegic arrest at which increased to 64% following reperfusion [n=7]. MI showed oedeme following ischeemia, and gross interfibrillar oedema following In contrast, of 40 patients undergoing heart surgery protected by cold significant incraese in water occurred in only one patient who died on table. NI enebles quentitetive meesurement of myocsrdiel water content end experimental situations and reveals, as the usual W/O measurements intraand interfibriltar distribution.
92EPICARDIAL DISTRIBUTION
IMAGE AND
ANALYSIS USING NADH-FLUORESCENCE
DESK-TOP COMPUTERPATTERN. R.Rosen,
COMPARISON
B.Panzner,
OF EPICARDIAL
W.Klaus.
FLOW
Pharmacol.
Inst.Univ.Kdln, Gleueler Str.24, D-5000 Kijln 41 Fluorescence pictures of epicardial surface of paced Lanqendorff-perfused rabbit hearts were taken on high sensitivity films (Kodak Tri-X Pan,27 DIN or' Polaroid Typ 667 36 DIN) with a Rolleiflex camera after flash excitation (Xe-flash;100 Joule;120 usec) triggered by the stimulator.Endogenous NADH-fluorescence and indicator fluorescence (fluorescein, short term infusion) were monitored as indicators for oxigenation and perfusion pattern of epicardial tissue resp.. Disturbancies in both parameters were induced by ligation of a branch of the left coronary artery.Pictures were digitized with respect to grey levels (O-255) using a video-A/D-converter (Hamamatsu) into 256 x 256 pixels and stored on floppy disks. Data processing on a 64K desk-top computer (Apple) was performed using fast machine routines embedded in Basic proqrams. Distribution curves of the grey values of all pixels or of selected areas were analysed by Fourier-analysis for determination of the ranqe of qrey values correspondinq to special structures and construction of topographic maps. The number of pixels'in a predetermined region of a defined grey-range directly corresponds .to the area. On account of the reproducibility of the video-A/D-conversion (+5$) direct comparison of different pictures like flow distribution pattern (indicator fluorescence) and metabolic parameters (NADH-fluorescence) of the same heart may be performed. Supported by the Deutsche Forschungsgesellschaft SFB 68/A13 93
CORONARY MICROANGIOGRAPHY IN THE GUINEA PIG. N. Flores, R. Davies, W. Penny, D. Sheridan. Departments of Cardiology and Radiology, Welsh National School of Medicine, Cardiff, United Kingdom. We have developed a microangiographic technique for studying the coronary circulation in isolated buffer perfused guinea pig hearts. After control perfusion or desired intervention perfusion is switched to a suspension of contrast medium in aqueous buffer prewarmed to 37'C. Optimum filling and contrast were achieved using 20% barium sulphate (Baritop G powder), 3% gelatin and 11, tween-40. After 3 minutes filling hearts are arrested in the ice cool aqueous buffer and fixed for 24 hours in buffered formaldehyde. Hearts were then sectioned transverselv (2 mm thickness) and X-rayed on fine grain film (DuPont NDT45) using an X-ray defraction tube with copper target at 30 kV. The radiographs obtained may be enlarged X20 without loss of clarity. The images obtained using this technique indicate that the coronary circulation in guinea pig hearts is usually balanced or left dominant. the left anterior descending coronary~artery is usually-intramyocardial, and most interestingly is invariably continuous with the posterior descending coronary artery, forming a large apical collateral vessel Preliminary studies indicate that pharmacologically induced (methoxamine lO%l) coronary constriction may also be readily identified using the technique. The technique should be widely applicable to the study of coronary arteries in small animals.
OEOEMA: A DUANTITATIVE METHOD FOR VISUALISING CHANGES IN DISTRIBUTION AND MYOCARDIAL WATER DURING EXPERIMENTAL AND CLINICAL PROCEDURES. S. Cardiothoracic Research Unit, The H.V. Braimbridge, J. Cheyen. St. Themes' Hospital, London SEI, U.K. of the Left ventricle 1LVI has been shown to occur during reparfusion arrest. Conventional wet end dry weight [W/O) analysis is not feasible We heve situation because of the smell size of tissue semples. a technique whereby a fresh cryostat section is placed under an microscope, en area of Longitudinal muscle fibres is photographed, the negative is passed under e scanning densitometer which records e trace Muscle fibres appear as peaks on the trace, height varying with weter fibres. the Troughs to beseline between muscle fibres, correspond to wetar. An integrator etteched to the densitometer gives a value for any area of tissue scenned. Normal ret LV has e water content of 76%. of rat LV samples ln=131 was assessed by W/O and by microscopic (MI); a coefficient of correletion of 0.67 was obtained. In isolated heerts W/D revealed 62% water following 35 minutes cardioplegic arrest at which increased to 64% following reperfusion [n=7]. MI showed oedeme following ischeemia, and gross interfibrillar oedema following In contrast, of 40 patients undergoing heart surgery protected by cold significant incraese in water occurred in only one patient who died on table. NI enebles quentitetive meesurement of myocsrdiel water content end experimental situations and reveals, as the usual W/O measurements intraand interfibriltar distribution.
92EPICARDIAL DISTRIBUTION
IMAGE AND
ANALYSIS USING NADH-FLUORESCENCE
DESK-TOP COMPUTERPATTERN. R.Rosen,
COMPARISON
B.Panzner,
OF EPICARDIAL
W.Klaus.
FLOW
Pharmacol.
Inst.Univ.Kdln, Gleueler Str.24, D-5000 Kijln 41 Fluorescence pictures of epicardial surface of paced Lanqendorff-perfused rabbit hearts were taken on high sensitivity films (Kodak Tri-X Pan,27 DIN or' Polaroid Typ 667 36 DIN) with a Rolleiflex camera after flash excitation (Xe-flash;100 Joule;120 usec) triggered by the stimulator.Endogenous NADH-fluorescence and indicator fluorescence (fluorescein, short term infusion) were monitored as indicators for oxigenation and perfusion pattern of epicardial tissue resp.. Disturbancies in both parameters were induced by ligation of a branch of the left coronary artery.Pictures were digitized with respect to grey levels (O-255) using a video-A/D-converter (Hamamatsu) into 256 x 256 pixels and stored on floppy disks. Data processing on a 64K desk-top computer (Apple) was performed using fast machine routines embedded in Basic proqrams. Distribution curves of the grey values of all pixels or of selected areas were analysed by Fourier-analysis for determination of the ranqe of qrey values correspondinq to special structures and construction of topographic maps. The number of pixels'in a predetermined region of a defined grey-range directly corresponds .to the area. On account of the reproducibility of the video-A/D-conversion (+5$) direct comparison of different pictures like flow distribution pattern (indicator fluorescence) and metabolic parameters (NADH-fluorescence) of the same heart may be performed. Supported by the Deutsche Forschungsgesellschaft SFB 68/A13 93
CORONARY MICROANGIOGRAPHY IN THE GUINEA PIG. N. Flores, R. Davies, W. Penny, D. Sheridan. Departments of Cardiology and Radiology, Welsh National School of Medicine, Cardiff, United Kingdom. We have developed a microangiographic technique for studying the coronary circulation in isolated buffer perfused guinea pig hearts. After control perfusion or desired intervention perfusion is switched to a suspension of contrast medium in aqueous buffer prewarmed to 37'C. Optimum filling and contrast were achieved using 20% barium sulphate (Baritop G powder), 3% gelatin and 11, tween-40. After 3 minutes filling hearts are arrested in the ice cool aqueous buffer and fixed for 24 hours in buffered formaldehyde. Hearts were then sectioned transverselv (2 mm thickness) and X-rayed on fine grain film (DuPont NDT45) using an X-ray defraction tube with copper target at 30 kV. The radiographs obtained may be enlarged X20 without loss of clarity. The images obtained using this technique indicate that the coronary circulation in guinea pig hearts is usually balanced or left dominant. the left anterior descending coronary~artery is usually-intramyocardial, and most interestingly is invariably continuous with the posterior descending coronary artery, forming a large apical collateral vessel Preliminary studies indicate that pharmacologically induced (methoxamine lO%l) coronary constriction may also be readily identified using the technique. The technique should be widely applicable to the study of coronary arteries in small animals.