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Correlation between a dry chemistry immunoassay and FPIA in therapeutic drug monitoring
Pharmacological
275
Research, Vol. 26, Supplement I, 1992
PHARMACOLOGICAL EVALUATION OF ELCATONIN: HYPOCALCAEMIC ACTIVITY IN RATS Giovanni Femi and Maurizio Valente ISF - Laboratories for Biomedical Research (SmithKline Beecham Italy) Via Leonardo da Vinci, 1 - 20090 Trezzano s/N (Milan) Italy Elcatonin (ECT) is a synthetic analogue of eel calcitonin, in which the disulfide bridge has been replaced by an ethylenic one. ECT is currently employed in therapy for the prevention and treatment of osteoporosis and other metabolic bone diseases, As a first index of its pharmacological activity, the ability of ECT and a number of other natural calcitonins to lower total serum calcium one hour post i.v. administration to intact rats was measured. The dose-response characteristics of the hypocalcaemic effect of ECT and the calcitonins were compared using a wide range of doses (3-30,000 rig/kg i.v.). Overall, the rank order of activity was ECT 2 eel = salmon 2 chicken > pig 2 man. The time course (30-180 n-tin) of the effect of ECI showed that the maximal effect was reached between 30 and 120 n-tin and this was delayed in time with increasing doses (3100 r&kg i.v.). In addition, the ability of ECT to counteract the hypercalcaemia induced by vitamin A analogues in thyroparathyroidectomized rats was assessed.Administration of daily doses of ECT, 240-2,400 n&g i.m., for three days prevented the increase in total semm calcium, in a dose dependent manner.
CORRELATION BETWEEN A DRY CHEMISTRY IMMUNOASSAY AND FPIA IN THERAPEUTIC DRUG MONITORING Domenico Capone, Nicola Pisanti and Vincenzo De Marino Dpt of Human Communicative Sciences-Sect of Pharmacology, II Faculty of Medicine - Naples A dry chemistry multilayer immunoassay technique on OPUS system (PB Diagnostics) has been recently developed for therapeutic drug monitoring. We compared the OPUS to the TDx (FPIA, Abbott) system in dosing carbamazepine, phenobarbital, phenytoin, theophylline and valproic acid. Using both methods we analyzed a total of 114 specimens during 3 months. Analyses were performed in duplicate and at the same time. Results are shown in the table. A good agreement (correlation coefficient between 0.95 and 1.00) between OPUS and TDx immunoassay was found for all drugs tested. Y
Carbamazepine Phenobarbital Phenytoin Theophylline Valproic acid y=OPUS ; x=TDx
0.985x-0.16 1.10x+0.69 1.02x+0.42 0.97x-0.27 0.99x+3.27
n 21 25 23 20 25
r 0.98 0.96 0.95 1.00 0.98
7 7.61 26.31 16.26 17.13 78.47
r 7.89 23.27 15.49 17.98 75.88
275
Research, Vol. 26, Supplement I, 1992
PHARMACOLOGICAL EVALUATION OF ELCATONIN: HYPOCALCAEMIC ACTIVITY IN RATS Giovanni Femi and Maurizio Valente ISF - Laboratories for Biomedical Research (SmithKline Beecham Italy) Via Leonardo da Vinci, 1 - 20090 Trezzano s/N (Milan) Italy Elcatonin (ECT) is a synthetic analogue of eel calcitonin, in which the disulfide bridge has been replaced by an ethylenic one. ECT is currently employed in therapy for the prevention and treatment of osteoporosis and other metabolic bone diseases, As a first index of its pharmacological activity, the ability of ECT and a number of other natural calcitonins to lower total serum calcium one hour post i.v. administration to intact rats was measured. The dose-response characteristics of the hypocalcaemic effect of ECT and the calcitonins were compared using a wide range of doses (3-30,000 rig/kg i.v.). Overall, the rank order of activity was ECT 2 eel = salmon 2 chicken > pig 2 man. The time course (30-180 n-tin) of the effect of ECI showed that the maximal effect was reached between 30 and 120 n-tin and this was delayed in time with increasing doses (3100 r&kg i.v.). In addition, the ability of ECT to counteract the hypercalcaemia induced by vitamin A analogues in thyroparathyroidectomized rats was assessed.Administration of daily doses of ECT, 240-2,400 n&g i.m., for three days prevented the increase in total semm calcium, in a dose dependent manner.
CORRELATION BETWEEN A DRY CHEMISTRY IMMUNOASSAY AND FPIA IN THERAPEUTIC DRUG MONITORING Domenico Capone, Nicola Pisanti and Vincenzo De Marino Dpt of Human Communicative Sciences-Sect of Pharmacology, II Faculty of Medicine - Naples A dry chemistry multilayer immunoassay technique on OPUS system (PB Diagnostics) has been recently developed for therapeutic drug monitoring. We compared the OPUS to the TDx (FPIA, Abbott) system in dosing carbamazepine, phenobarbital, phenytoin, theophylline and valproic acid. Using both methods we analyzed a total of 114 specimens during 3 months. Analyses were performed in duplicate and at the same time. Results are shown in the table. A good agreement (correlation coefficient between 0.95 and 1.00) between OPUS and TDx immunoassay was found for all drugs tested. Y
Carbamazepine Phenobarbital Phenytoin Theophylline Valproic acid y=OPUS ; x=TDx
0.985x-0.16 1.10x+0.69 1.02x+0.42 0.97x-0.27 0.99x+3.27
n 21 25 23 20 25
r 0.98 0.96 0.95 1.00 0.98
7 7.61 26.31 16.26 17.13 78.47
r 7.89 23.27 15.49 17.98 75.88