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Correlation between serum selenium and liver fibrogenesis
252
CORRELATION BETWEEN SERUM SELENIUM AND LIVER FIBROGENESIS.
G a b r ~ e ~ GB. C~arX~ M. B e ~ o l a G. Capra F. Barace~no F. Bonazzi L. FaZezza GC. anti Co,,w.ocher R. C ~ e d r e Patologia Medica e Chim/ca C l l n l c a , U ~ Z v ~ i t ~ di Verona. Low serum s ~ Z e n ~ (S-Se) i s associated with d ~ e r e ~ t pathologieaZ eondi~ons in hamans, such as m a l i g n a n c y , ~ o v a s c u l o J t and / / v e t disease~. The ~ o~ p r i e s t study was to e l a r ~ y whether a r e l a t i o ~ h i p e x i s t s between Se and ae..tive ~ b r o s i a i n ~ c o h o l i c l i v e r disease~ (ALD). In 62 pat.~ents w/th ALD (41 c i r r h o s i s (C), 21 non e ~ o t ~ c ALD (NC}} we studied, s ~ Se concentration by proton induced X ray ~ s i o n , p/asma ~ b r o n e e ~ n (FN) by immunon~ph~om~try and N-peptv~de or( procollagen I l l (NP3P) by radioX~muno assay. S-Se in C was lower than in NC p ~ e n t s and in 47 controls (47.3, SD 15.0, 56.8, SD 14.5; 72.4, SD 12.6 ng/ml r ~ p e c t ~ v ~ y ; F=36.16}. FN was lower i n C (23.2, SD 11.8 mg/dl} than in co~trols (38.1, SD 9.6 mg/dl) (F= 26.8) whi~e NC were within norm~ range (35.6, SD 11.1 mg/dl). NP3P was i n ~ e a s ~ in C (63.5, SD 120.7 ng/ml) compared with controls (13.4, SD 2.51 ng/ml) and NC (17.2, SD 8.7 ng/ml} (F= 6.40) In ~ i r r h o t i c pat/ents NP3P was n e g a t i v ~ y correZated with S-Se (r=-0.409, p 50 ng/ml had a s i g n i ~ c a ~ t l y higher NP3P 133.16 SD 19.04 us 20.3, SD 12.8 ng/ml, p
253
TAURINE AND GLYCINE CONJUGATIONAND SULFATION OF LITHOCHOLICACID IN THE LIVER CELL P.Galle, L.Theilmann, R.Raedsch, G.RudoIph, A.Stiehl, Department of Medicine, GI Unit, University of Heidelberg, Med. Univ.-Klinik, Heidelberg.
In man and rat l i t h o c h o l i c acid is conjugated at i t s carboxyl group with the amino acids taurine and glycine and in part is sulfated at the 3-hydroxyl group. The present study was performed to find out whether sulfated bile acids are always conjugated with taurine or glycine and whether taurine and glycine conjugation of sulfates parallels that of the non-sulfates. Methods: Rat hepatocytes were obtained after perfusion of the l i v e r s with collagenase and were cultured on collagen coated dishes (Meth.Cell.Biol.13,29,1976). 24 hours later 10 nmoles 14C-lithocholic acid (5700 DPM/nmol) was added as marker. Bile acids were separated by column chromatography (Clin.Chim.Acta 123,275,1982). Results: After incubation for 1 hour 0.575 nmol/lOOcells were sulfated; of these 80.7 % were taurine and 2.4 % glycine conjugated, 16.9% were not amidated. 9.11 nmol/lO6 cells were amidated but not sulfated~ of these 93.8% were taurine and 6.2% glycine conjugated. After 8 hours 1.853 nmol/lOo cells were sulfated; of these 93.8% were taurine and 4.6% glycine conjugated and 1.6% were not amidated. 7.530 nmol/lO6 cells were amidated but not sulfated; of these 93.6% were taurine and 6.4% glycine conjugated. The results indicate that bile acids which are sulfated in the l i v e r cell are mostly also conjugated with taurine or glycine. The ratio of taurine to glycine conjugation of bile acid sulfates and non-sulfates was similar. We conclude: 1. Taurine and glycine conjugation of sulfated and non-sulfated bile acids follow the same principles 2. Taurine is the prefered substrate also for sulfated bile acids. 3. The r e l a t i v e l y large amounts of non amidated bile acid sulfates in intestine and urine (Hepatology 5,492,1985) are not formed by the l i v e r but most l i k e l y by bacteria in the intestine.
S132
CORRELATION BETWEEN SERUM SELENIUM AND LIVER FIBROGENESIS.
G a b r ~ e ~ GB. C~arX~ M. B e ~ o l a G. Capra F. Barace~no F. Bonazzi L. FaZezza GC. anti Co,,w.ocher R. C ~ e d r e Patologia Medica e Chim/ca C l l n l c a , U ~ Z v ~ i t ~ di Verona. Low serum s ~ Z e n ~ (S-Se) i s associated with d ~ e r e ~ t pathologieaZ eondi~ons in hamans, such as m a l i g n a n c y , ~ o v a s c u l o J t and / / v e t disease~. The ~ o~ p r i e s t study was to e l a r ~ y whether a r e l a t i o ~ h i p e x i s t s between Se and ae..tive ~ b r o s i a i n ~ c o h o l i c l i v e r disease~ (ALD). In 62 pat.~ents w/th ALD (41 c i r r h o s i s (C), 21 non e ~ o t ~ c ALD (NC}} we studied, s ~ Se concentration by proton induced X ray ~ s i o n , p/asma ~ b r o n e e ~ n (FN) by immunon~ph~om~try and N-peptv~de or( procollagen I l l (NP3P) by radioX~muno assay. S-Se in C was lower than in NC p ~ e n t s and in 47 controls (47.3, SD 15.0, 56.8, SD 14.5; 72.4, SD 12.6 ng/ml r ~ p e c t ~ v ~ y ; F=36.16}. FN was lower i n C (23.2, SD 11.8 mg/dl} than in co~trols (38.1, SD 9.6 mg/dl) (F= 26.8) whi~e NC were within norm~ range (35.6, SD 11.1 mg/dl). NP3P was i n ~ e a s ~ in C (63.5, SD 120.7 ng/ml) compared with controls (13.4, SD 2.51 ng/ml) and NC (17.2, SD 8.7 ng/ml} (F= 6.40) In ~ i r r h o t i c pat/ents NP3P was n e g a t i v ~ y correZated with S-Se (r=-0.409, p
253
TAURINE AND GLYCINE CONJUGATIONAND SULFATION OF LITHOCHOLICACID IN THE LIVER CELL P.Galle, L.Theilmann, R.Raedsch, G.RudoIph, A.Stiehl, Department of Medicine, GI Unit, University of Heidelberg, Med. Univ.-Klinik, Heidelberg.
In man and rat l i t h o c h o l i c acid is conjugated at i t s carboxyl group with the amino acids taurine and glycine and in part is sulfated at the 3-hydroxyl group. The present study was performed to find out whether sulfated bile acids are always conjugated with taurine or glycine and whether taurine and glycine conjugation of sulfates parallels that of the non-sulfates. Methods: Rat hepatocytes were obtained after perfusion of the l i v e r s with collagenase and were cultured on collagen coated dishes (Meth.Cell.Biol.13,29,1976). 24 hours later 10 nmoles 14C-lithocholic acid (5700 DPM/nmol) was added as marker. Bile acids were separated by column chromatography (Clin.Chim.Acta 123,275,1982). Results: After incubation for 1 hour 0.575 nmol/lOOcells were sulfated; of these 80.7 % were taurine and 2.4 % glycine conjugated, 16.9% were not amidated. 9.11 nmol/lO6 cells were amidated but not sulfated~ of these 93.8% were taurine and 6.2% glycine conjugated. After 8 hours 1.853 nmol/lOo cells were sulfated; of these 93.8% were taurine and 4.6% glycine conjugated and 1.6% were not amidated. 7.530 nmol/lO6 cells were amidated but not sulfated; of these 93.6% were taurine and 6.4% glycine conjugated. The results indicate that bile acids which are sulfated in the l i v e r cell are mostly also conjugated with taurine or glycine. The ratio of taurine to glycine conjugation of bile acid sulfates and non-sulfates was similar. We conclude: 1. Taurine and glycine conjugation of sulfated and non-sulfated bile acids follow the same principles 2. Taurine is the prefered substrate also for sulfated bile acids. 3. The r e l a t i v e l y large amounts of non amidated bile acid sulfates in intestine and urine (Hepatology 5,492,1985) are not formed by the l i v e r but most l i k e l y by bacteria in the intestine.
S132