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Corrigendum to “β-Glucosylation of chitooligomers by galactosyltransferase” [Carbohydr. Res. 305 (1998) 517–523]
Carbohydrate Research 401 (2015) 132
Contents lists available at ScienceDirect
Carbohydrate Research journal homepage: www.elsevier.com/locate/carres
Corrigendum
Corrigendum to ‘‘b-Glucosylation of chitooligomers by galactosyltransferase’’ [Carbohydr. Res. 305 (1998) 517–523] Vladimír Krˇen a,⇑, Jana Dvorˇáková a, Ulrike Gambert b, Petr Sedmera a, Vladimír Havlícˇek a, Joachim Thiem b, Karel Bezouška c a
Institute of Microbiology, Academy of Sciences of the Czech Republic, Laboratory of Biotransformation, Vídenˇská 1083, Prague 4 CZ 142 20, Czech Republic Institute of Organic Chemistry, University of Hamburg, Martin-Luther-King-Platz 6, Hamburg D-20146, Germany c Faculty of Science, Charles University Prague, Department of Biochemistry, Hlavova 8, Prague 2 CZ 128 40, Czech Republic b
This paper describes the finding that galactosyltransferase from bovine milk is able to utilise UDP-Glc to transfer Glc onto GlcNAc and chitooligomers [–b-GlcNAc-(1–4)–]n, n = 2–4. In this work four novel oligosaccharides were prepared in good yield; all compounds were carefully characterized by the NMR and MS spectra and chiroptical methods. This work also demonstrated for the first time the ability of galactosyltransferase to transfer glucose on a semipreparatory scale. b-Glucosylated products were used in binding studies with NKR-P1A protein cloned from rat natural killer cells. In this corrigendum we now report that the data on the NKRP1A protein binding to the carbohydrates cannot be reproduced (cf.
DOI of original article: http://dx.doi.org/10.1016/S0008-6215(97)00248-6
⇑ Corresponding author.
E-mail address: [email protected] (V. Krˇen). http://dx.doi.org/10.1016/j.carres.2014.05.019
re-evaluation study1) and, therefore, no longer should be regarded as valid. More specifically, we retract all results contained in and related to the Figure 1 and all discussions concerning this. Reference 1. Rozbesky´, D.; Krejzová, J.; Krˇenek, K.; Prchal, J.; Hrabal, R.; Kozˇíšek, M.; Weignerová, L.; Fiore, M.; Dumy, P.; Renaudet, O.; Krˇen, V. Reevaluation of Binding Properties of Recombinant Lymphocyte Receptors NKR-P1A and CD69 to Chemically Synthesized Glycans and Peptides Int. J. Mol. Sci. 2014, 15, 1271–1283.
Contents lists available at ScienceDirect
Carbohydrate Research journal homepage: www.elsevier.com/locate/carres
Corrigendum
Corrigendum to ‘‘b-Glucosylation of chitooligomers by galactosyltransferase’’ [Carbohydr. Res. 305 (1998) 517–523] Vladimír Krˇen a,⇑, Jana Dvorˇáková a, Ulrike Gambert b, Petr Sedmera a, Vladimír Havlícˇek a, Joachim Thiem b, Karel Bezouška c a
Institute of Microbiology, Academy of Sciences of the Czech Republic, Laboratory of Biotransformation, Vídenˇská 1083, Prague 4 CZ 142 20, Czech Republic Institute of Organic Chemistry, University of Hamburg, Martin-Luther-King-Platz 6, Hamburg D-20146, Germany c Faculty of Science, Charles University Prague, Department of Biochemistry, Hlavova 8, Prague 2 CZ 128 40, Czech Republic b
This paper describes the finding that galactosyltransferase from bovine milk is able to utilise UDP-Glc to transfer Glc onto GlcNAc and chitooligomers [–b-GlcNAc-(1–4)–]n, n = 2–4. In this work four novel oligosaccharides were prepared in good yield; all compounds were carefully characterized by the NMR and MS spectra and chiroptical methods. This work also demonstrated for the first time the ability of galactosyltransferase to transfer glucose on a semipreparatory scale. b-Glucosylated products were used in binding studies with NKR-P1A protein cloned from rat natural killer cells. In this corrigendum we now report that the data on the NKRP1A protein binding to the carbohydrates cannot be reproduced (cf.
DOI of original article: http://dx.doi.org/10.1016/S0008-6215(97)00248-6
⇑ Corresponding author.
E-mail address: [email protected] (V. Krˇen). http://dx.doi.org/10.1016/j.carres.2014.05.019
re-evaluation study1) and, therefore, no longer should be regarded as valid. More specifically, we retract all results contained in and related to the Figure 1 and all discussions concerning this. Reference 1. Rozbesky´, D.; Krejzová, J.; Krˇenek, K.; Prchal, J.; Hrabal, R.; Kozˇíšek, M.; Weignerová, L.; Fiore, M.; Dumy, P.; Renaudet, O.; Krˇen, V. Reevaluation of Binding Properties of Recombinant Lymphocyte Receptors NKR-P1A and CD69 to Chemically Synthesized Glycans and Peptides Int. J. Mol. Sci. 2014, 15, 1271–1283.