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Corrigendum to “organization of NMDA receptors at extrasynaptic locations”
Neuroscience 176 (2011) 472
CORRIGENDUM CORRIGENDUM TO “ORGANIZATION OF NMDA RECEPTORS AT EXTRASYNAPTIC LOCATIONS” [Neuroscience 167 (2010) 68 – 87] R. S. PETRALIA,a* Y. X. WANG,a F. HUA,a Z. YI,a A. ZHOU,a L. GE,a F. A. STEPHENSONb AND R. J. WENTHOLDa† a Laboratory of Neurochemistry, National Institute on Deafness and Other Communication Disorders/National Institutes of Health (NIDCD/NIH), Bethesda, MD, USA b School of Pharmacy, University of London, 29/39 Brunswick Square, London WC1N 1AX, UK
On page 72, Fig. 2 legend, the author noted an error in the text corresponding to panels G and H. The correct figure legend is shown below. High magnifications of parts of micrographs (additionally processed for brightness and contrast) from triple immunofluorescence labeling of hippocampal cultures with (1) live surface labeling of NR2A (red; 555 for D, E; 647 [STED] for G, H) or NR2B (red-555; A–C, F), (2) the presynaptic marker VGLUT (blue; 647 for A, D–F; 488 for B, C, G, H) and (3) a third protein (green; 488 for A, D–F; 555 for G, H; 647 for B, C) including PSD-95/93 (B, C), SAP102 (Neuromab; A, D, E, G, H), or catenin (F). (A) NR2B/SAP102: note colocalization in areas devoid of VGLUT labeled terminals (shown also alone in the inset)-some appear to be real associations (*) while others may be coincidental (**). (B, C) NR2B/PSD-95/93: NR2B forms in a perisynaptic ring (**) around synaptic PSD-95/93 and the terminal, and forms a ring of three puncta around an extrasynaptic punctum of PSD-95/93 (*); (C) is the same image, with the NR2B labeling shown in higher contrast. (D) NR2A/SAP102: this is an enlarged region found along a thin, distal dendrite. Note how NR2A labeling is spread in the perisynaptic regions surrounding two synapses (*)-SAP102 forms around the enlargement in conjunction with both the synaptic and extrasynaptic (**) NR2A; the right image is a high contrast version of the left one. In the diagram, the outline of the thin dendrite is shown as black lines. (E) NR2A/SAP102: NR2A forms a partial perisynaptic ring around synaptic SAP102 (**); in another example (*), the colocalization of NR2A with an elongate punctum of SAP102 may be coincidental (see text). (F) NR2B/catenin: 2 NR2B puncta, one synaptic and one extrasynaptic, associate with an elongate punctum of catenin (*; seen alone in the inset). (G, H) NR2A[STED]/SAP102: synaptic or perisynaptic puncta (*) of NR2A can be seen at slightly higher resolution than in the confocal images. Scale bars are 500 nm. (Available online 28 December 2010)
DOI of original article: 10.1016/j.neuroscience.2010.01.022. † Deceased. *Corresponding author. Tel: ⫹1-301-496-3804. E-mail address: [email protected] (R. S. Petralia). 0306-4522/11 $ - see front matter. Published by Elsevier Ltd on behalf of IBRO. doi:10.1016/j.neuroscience.2010.12.020
472
CORRIGENDUM CORRIGENDUM TO “ORGANIZATION OF NMDA RECEPTORS AT EXTRASYNAPTIC LOCATIONS” [Neuroscience 167 (2010) 68 – 87] R. S. PETRALIA,a* Y. X. WANG,a F. HUA,a Z. YI,a A. ZHOU,a L. GE,a F. A. STEPHENSONb AND R. J. WENTHOLDa† a Laboratory of Neurochemistry, National Institute on Deafness and Other Communication Disorders/National Institutes of Health (NIDCD/NIH), Bethesda, MD, USA b School of Pharmacy, University of London, 29/39 Brunswick Square, London WC1N 1AX, UK
On page 72, Fig. 2 legend, the author noted an error in the text corresponding to panels G and H. The correct figure legend is shown below. High magnifications of parts of micrographs (additionally processed for brightness and contrast) from triple immunofluorescence labeling of hippocampal cultures with (1) live surface labeling of NR2A (red; 555 for D, E; 647 [STED] for G, H) or NR2B (red-555; A–C, F), (2) the presynaptic marker VGLUT (blue; 647 for A, D–F; 488 for B, C, G, H) and (3) a third protein (green; 488 for A, D–F; 555 for G, H; 647 for B, C) including PSD-95/93 (B, C), SAP102 (Neuromab; A, D, E, G, H), or catenin (F). (A) NR2B/SAP102: note colocalization in areas devoid of VGLUT labeled terminals (shown also alone in the inset)-some appear to be real associations (*) while others may be coincidental (**). (B, C) NR2B/PSD-95/93: NR2B forms in a perisynaptic ring (**) around synaptic PSD-95/93 and the terminal, and forms a ring of three puncta around an extrasynaptic punctum of PSD-95/93 (*); (C) is the same image, with the NR2B labeling shown in higher contrast. (D) NR2A/SAP102: this is an enlarged region found along a thin, distal dendrite. Note how NR2A labeling is spread in the perisynaptic regions surrounding two synapses (*)-SAP102 forms around the enlargement in conjunction with both the synaptic and extrasynaptic (**) NR2A; the right image is a high contrast version of the left one. In the diagram, the outline of the thin dendrite is shown as black lines. (E) NR2A/SAP102: NR2A forms a partial perisynaptic ring around synaptic SAP102 (**); in another example (*), the colocalization of NR2A with an elongate punctum of SAP102 may be coincidental (see text). (F) NR2B/catenin: 2 NR2B puncta, one synaptic and one extrasynaptic, associate with an elongate punctum of catenin (*; seen alone in the inset). (G, H) NR2A[STED]/SAP102: synaptic or perisynaptic puncta (*) of NR2A can be seen at slightly higher resolution than in the confocal images. Scale bars are 500 nm. (Available online 28 December 2010)
DOI of original article: 10.1016/j.neuroscience.2010.01.022. † Deceased. *Corresponding author. Tel: ⫹1-301-496-3804. E-mail address: [email protected] (R. S. Petralia). 0306-4522/11 $ - see front matter. Published by Elsevier Ltd on behalf of IBRO. doi:10.1016/j.neuroscience.2010.12.020
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