Cumulative subject index for volumes 176–183

ANALYTICAL

183,335-375

BIOCHEMISTRY

(1989)

Cumulative

Subject Index for Volumes

176-l

ACV synthetase o-phthaldialdehyde-derivatized,

A

HPLC

(Strepfomyces

clauuligeru~),

178,399

Absorbance ultraviolet, fying 180,314 Acetic acid -urea-Triton sional Acetylcholine HPLC and in solutions bilized

with diaminobenzoic acid fluorescence assay, and ascertaining purity of DNA preparation

X-100, in protein gel electrophoresis,

gel electrophoresis 177,388

after

GC-MS (human, rat), 180,85 and tissue extracts, assay by flow injection enzymes and fluorometric or luminometric

in quanti(maize),

two-dimen-

with immodetection,

176,221 Acetylcholine nicotinic,

receptors fiber-optic

evanescent

fluorosensor,

characterization,

182,353 Acetylcholinesterase inhibition in microassay of low levels of toxic organophosphorus compounds, 180,380 molecular forms in skeletal muscle, assay in crude extracts (rat),

180,227 Acetyl-CoA ‘Y-labeled, synthesis Acetylcysteine adducts of xenobiotics,

from

sodium

structural

acetate, analysis

176,82 by FAB

and tandem

derived isoindoles, formation and degradation, tometric study, 180,172 N-Acetylglucosamine-6-sulfatase estimation with 0-(ol-N-acetylglucosamine-6-sulfate)-(1 ]6-aH]idonic acid: diagnosis of SanfilippoD

kinetic-spectropho-

syndrome

+ 3)L(human),

176,244 N-Acetyl-/3-D-glucosaminidase spectrophotometric assay with 3,4-dinitrophenyl N-acetyl-fl-D-glucosaminide, 183,245 N-Acetyl-P-D-hexosaminidase spectrophotometric assay with 3,4-dinitrophenyl N-acetyl-fl-D-glucosaminide, 183,245 N-Acetylneuraminic acid and mono-0-acetylated derivatives, assay in sera by HPLC with fluorometry (human, rat), 179,162 Acidemia lactic, differential diagnosis by determination of [U-n’C]glucose turnover into various metabolite pools (human), 176,382 al-Acid glycoprotein galactosylation variants, immunofluorometric assay with immobilized Bandeiraea .simpEicifoZin II lectin, 177, 383 Acid phosphatase visualization on nitrocellulose after electroblotting of polyacrylamide gels, 179,194 Actin binding proteins, two-dimensional gel electrophoresis with SDS (rabbit skeletal muscle), 178,32 histidine residue methylation, reverse-phase HPLC analysis (chicken, mammals), 178,194 numbers

indicate

appropriate

0003s2697/89 $3.00 Copyright 0 1989 by Academic Press, Inc. All rights of reproduction in any form reserved.

volume;

lightface

Acylcarnitines in blood, tissues, and urine, FAB/MS and liquid chromatography/ MS (human), 180,331 and free carnitine, in urine, assay by FAR/MS (human), 176,85 Acylphosphatase activity staining after gel electrophoresis, in analysis of isozyme tissue distribution (chicken, pig), 183,46 Adenosine deaminase fluorescence-grade, production by laser photobleaching (bovine intestinal mucosa), 181,383 Adenosine %phosphosulfate nonhydrolyzable analog /s’-methylene APS, synthesis and structural confirmation, 177,67 S-Adenosylmethionine in small samples of cerebrospinal fluid and brain extract, HPLCelectrochemical assay (human, mouse), 181,331 Adipose tissue lipolysis studies, application of glycerol luminometric kinetic assay (human), 177,132 cu,-Adrenergic receptors hepatic, solubilization and partial purification by CHAPS, role of glycerol, sodium phosphate, and sodium chloride (rat), 176,

375

MS,

183.94

’ Boldface

83'

numbers

Adsorbents thiophilic, for one-step HPLC purification of monoclonal antibodies, 180,66 Adsorption proteins onto glass surfaces, effect on intensity of circular dichroism spectra, 177,178 salt-promoted thiophilic, proteins onto sulfur-containing agarose, implications for covalent chromatography, 176,330 Aeration and mixing, cell and organelle suspensions during NMR, device,

182,151 Affinity chromatography affinity ligands immobilized on 3-isothiocyanatopropyltriethoxysilane-activated nonporous monodisperse silicas, 179,171 boronic acids for, spectral methods for determinations of ionization and diol-binding constants, 178, 125 GMP-based, mutant hypoxanthine phosphoribosyltransferase,

178,148 high-performance immobilized-metal-ion, in evaluation of peptide interaction with Cu(II), Ni(II), and Zn(II), 183,159 high-performance liquid, glucose-containing tetrasaccharide (human serum, urine), 182,48 and ion-pair HPLC, in analysis of tissue folate composition (bovine, human, rat), 182,84 polyclonal antisera on keratin affinity columns, improvement of immunoblot quality (human, rat), 182,193 protein-serine kinases with synthetic peptide substrates (rabbit muscle), 180,237 single-stranded DNA for dideoxy sequencing after polymerase chain reaction, 178,239 indicate

pagination. 335

336

CUMULATIVE

SUBJECT

Affinity gels antibody-linked, elecroelution of immobilized antigens, 177,314 Agar media screening of mutant penicillin producer strains on, application of spectrophotometric assay of hydroxylated by-products of penicillin V fermentation, 179,288 Agarose gels acidic, for isoelectric focusing, calibration with parvalbumins, 178, 121 DNA-intercalated ethidium bromide on, quantitative fluorescence,

181,197 electrophoretic isolation of high-molecular-weight DNA, 181, 12 with ethidium bromide, plate assay for cDNA synthesis, 178, 269 sulfur-containing, salt-promoted thiophilic adsorption of proteins, implications for covalent chromatography, 176,330 Albumin serum, see Serum albumin Alcohol dehydrogenase in amplification of spectrophotometric assay for NAD+ and NADH,

179,382 class I, II, and III isozymes, ELBA (human liver), 176,48 in continuous spectrophotometric assay of phospholipase Az hydrolyzing plasmalogens, 176,180 in detection of ethyl alcohol in urine, media, utility of superabsorbent polymers (human), 182,257 fluorometric assay of isozymes (human), 178,57 Alcohol oxidase solid, in calorimetric enzymatic assay of ethanol and formaldehyde vapors, 182,109 Aldose reductase immunoquantitation in tissues, ELISA (human), 176,66 Alkaline phosphatase detection by dual-enzyme cascade, 179,229 -oligonucleotide hybridization probes, preparation with 5’-oligonucleotide hydrazide derivatives, 178,43 Alkylation sulfhydryl groups by N-iodoacetyltyramine, in iz51 labeling of compounds, 179,389 Alkyl esters preparation from peptides by liquid secondary-ion mass spectrometry, 182,165 Alkyl sulfates in protein solutions, analysis by isocratic and gradient ion chromatography, 182,103 Alumina -phosphate complexes for immobilization of biomolecules, review,

180,l Amination reductive, in immobilization of proteins on oxidized crosslinked Sepharose preparations, 180,114 Amines trace, dopamineand norepinephrine-related, assay by prechromatographic derivatization with naphthalene-2,3-dicarboxaldehyde (human urine), 180,279 Amino acid analysis in determination of molecular ratio of conjugates between proteins using least-squares computer method, 179,158 direct, proteins electroblotted onto polyvinylidene difluoride membranes from SDS-polyacrylamide gel, 181,75 free amino acids in whole peanut seeds, 180,264 high-sensitivity, by derivatization with o-phthalaldehyde and 9fluorenylmethyl chloroformate and fluorescence detection: application to protein structure determination, 178,227 orthophthalaldehyde as reagent, instability, 181,135 peptides electroblotted onto polyvinylidene difluoride membranes from Tricine-SDS-PAGE: application to human complement component C3,181,33

INDEX

phenylthiohydantoin-amino acid, subpicomole chromatographic, evaluation, 183,290 phosphorylated proteins immobilized on polyvinylidene difluoride membrane, 177,407 at picomole level, application of reverse-phase HPLC of dimethylaminobenzene sulfonyl and thiocarbamoyl derivatives, 178, 107 by precolumn HPLC with l-naphthylisocyanate as derivat; ‘ation reagent, 180,291 proteins electroblotted onto polyvinylidene difluoride membr .;nes,

179,50 by reverse-phase HPLC trophenyl-5-L-alanine Amino acid decarboxylase aliphatic forms, ion-pair

after derivatization amide, 178,17 reverse-phase

with

HPLC

1-fluoro-2,4-dini-

with

fluorometry,

179,182 spectrophotometric indicator assay (Escherichiu coli), 181,59 L-Amino-acid oxidase in enzyme cycling assay for phenylpyruvate in tissue extracts (rat),

183,210 Amino acid receptors excitatory, autoradiographic analysis with L-[3H]glutamate (rat brain), 177,150 Amino acids derivatives, molecular imprinting at O’C with photolytic homolysis of azobisnitriles, 177, 144 derived isoindoles, formation and degradation, kinetic-spectrophotometric study, 180,172 4-N,N-dimethylaminoazobenzene C’-thiohydantoin derivatives, HPLC analysis at sub-picomole levels: simultaneous manual sequencing of picomole quantities of several polypeptides, 177, 46 dimethylaminobenzene sulfonyl and thiocarbamoyl derivatives, reverse-phase HPLC for amino acid analysis and microsequencing at picomole level, 178,107 free assay in whole peanut seeds, 180,264 crystalline N-tert-butyloxycarbonyl 0-phenacyl derivatives, gas chromatographic analysis (fruit fly), 176,368 HPLC with electrochemical detection after precolumn derivatization with naphthalenedialdehyde/cyanide, 178,202 labile, high recovery during hydrolysis of proteins and peptides in hermetically sealed microcapillary tube (bovine, chicken), 182, 383 phenylthiocarbamyl, identification for compositional analysis by thermospray liquid chromatography-MS, 176,269 o-phthalaldehyde derivatives, formation and instability, review,

178,l u-Amino acids boroxazolidone formation, evaluation of stability, 178,276 &(L-u-Aminoadipyl)-L-cysteinyl-D-valine synthetase, see ACV synthetase y-Aminobutyric acid radiolabeled, release from synaptosomes in vitro, measurement by superfusion system on subsecond time scale (rat brain), 178,8 L-cu-Amino-c-caprolactam hydrolase spectrophotometric assay (Cryptococcus hrentii), 181,297 Amino groups on polymer supports, spectrophotometric estimation, 180,253; er-

ratum,183,197 Aminopeptidase active-site titrations, oxazolones as substrates, 181,321 4-(Aminosulfonyl)-7-Auoro-2,1,3-benzoxadiazole fluorescent labeling of cyst(e)ine-containing proteins prior to structural analyses, 180,231 Aminotransferase one- and two-step fluorometric assay methods with glutamate dehydrogenase (mouse, rat), 182,129

CUMULATIVE

SUBJECT

spectrophotometric estimation with algal NADPH-linked glutamate dehydrogenase, 180,99 Ammonia assimilating enzymes from cyanobacteria, HPLC assay in uitro and in situ, 180,152 Ammonium sulfate -t-butanol-water, in three-phase partitioning isolation of cathepsin D (bovine spleen), 180,169 AMI‘ (adenosine 5’-monophosphate) FAH/MS, low-nanogram detection, 178,102 Amperometric detection with immobilized enzyme assay, creatine kinase, 176,234 pulsed and high-pH anion-exchange chromatography, in separation of branched sialylated oligosaccharides from fetuin (bovine),

182,l and membrane reactor, in reverse-phase HPLC assay for cyclodextrins and branched forms, 179,336 Ampholine columns, in preparative isoelectric focusing of leukoregulin, comparison with immobiline polyacrylamide gels, 177,358 pH gradient, in preparative free solution isoelectric focusing of uroporphyrinogen I synthase (human erythrocytes), 182,217 Ampicillin resistance gene in chimeric plasmids of yeast cells: iodometric detection of p-lactamase, 177,165 Amyloid fibrils fluorometric assay in vitro with thioflavine T (murine liver), 177, 244 Androgen aromatase fast liquid chromatography (human placenta), 181,167 Angiotensin-converting enzyme, see Dipeptidyl carboxypeptidase I Antibiotics isotopic labeling by reductive methylation, 181,90 Antibodies affinity-purified, in tyrosine hydroxylase spot blot immunoassay (bovine, rat), 181,259 -antigen interactions, immunoassay with surface-enhanced Raman spectroscopy, 182,388 anti-p2lc-N-ra.s-specific, generation and characterization, eluted from nitrocellulose blots, assay with gold reagents: tion to electron microscopic immunocytochemistry

180,158 applica(rabbit),

183,225 immobilization on silica with thiol-terminal silanes and heterobifunctional crosslinkers, 178,408 labeling with metal ions: synthesis of dlethylenetriaminetetraacetic acid activated N-benzyl derivatives as alternative reagents,

176,319 linked

affinity

matrices,

electroelution

of immobilized

antigens,

177,314 Antigens -antibody interactions, immunoassay with surface-enhanced Raman spectroscopy, 182,388 epitopes, and mRNAs, simultaneous localization: extracellular matrix gene expression by cultured cells (human), 178,184 immobilized on antibody-linked affinity matrices, electroelution,

177,314 labeled,

immunoprecipitation

with

Eupergit

ClZ

acrylic

beads,

180,72 simultaneous quantification by immunoblot assay (rat brain), 177,203 visualization on nitrocellulose by oxidative coupling reaction of N,N’-dimethyl-p-phenylendiamine and 4-chloro-1-naphthol,

183,9 Antioxidants scavenging activity, qualitative fluorometric tification as efficient scavenger, 180,326

assay: ovothiol

A iden-

337

INDEX

Antisera polyclonal, ment

chromatography of immunoblot

on keratin affinity quality (human, rat),

columns: 182,193

improve-

Aphidicolin in large-scale synchronization of L1210 cell cultures, and study of subsequent cell cycle regulation of thymidylate synthase and dihydrofolate reductase (mouse), 182,338 Apolipoprotein A-I adsorption onto glass surfaces, effect on intensity of circular dichroism spectra (human), 177,178 purification from precipitates obtained by cold ethanol fractionation of plasma (human), 178,301 Apolipoprotein A-II purification from precipitates obtained by cold ethanol fractionation of plasma (human), 178,301 Apolipoprotein B different species, SDS-agarose gel electrophoresis and immunoblotting, 181,86 Apolipoprotein E adsorption onto glass surfaces, effect on intensity of circular dichroism spectra (human), 177,178 Aqueous two-phase systems liquid-liquid partition chromatographic fractionation of immunoglobulins (human, rabbit), 183,305 Arachidonic acid and phospholipid classes, HPLC separation on cyanopropyl columns (bovine, chicken, rat, soybean), 182,32 Arginine decarboxylase spectrophotometric indicator assay (Escherichia coli), 181,59 Arsanilic acid diazotized, modified amino acids in proteins and peptides, identification, 177,183 Arsenazo III loaded liposomes, in quantitation of Ca2’ uptake, 177,402 Arylesterase hydrolysis of active metabolites of chlorpyrifos and parathion, spectrophotometric assays (human plasma), 180,242 Arylsulfotransferase purified, assay suitability for reactions yielding unstable sulfuric acid esters, 183,320 Ascorbic acid assay by HPLC with coulometric electrochemical detection (bovine, human), 181,276 effects on deconjugation of noradrenaline and dopamine in plasma (human), 182,262 Asparagine linked oligosaccharides of glycoproteins, epitopes on, determination, 182,200 Astrocytes in monolayer culture, protein determination by bicinchoninic acid method (rat brain), 177,41 ATPase H+-, plasma membrane-associated, analysis by SDS-PAGE (Neurospora crassa), 176,284 Na+,K+directional immobilization by wheat germ agglutinin linked to microtiter plates, 180,362 total activity, assay in isolated or cultured cells (rat), 183,215 ATP-sulfurylase, see Sulfate adenylyltransferase Atria1 natriuretic factor in chromaffin cell cultures, bidimensional reverse-phase HPLC (bovine), 179,24 AuroDye comparison with FerriDye and India ink staining of proteins in subnanogram and nanogram range, 182,44 Autoradiography excitatory amino acid receptor analysis with radiolabeled glutamate, biochemical characterization, 177,150

338

CUMULATIVE

SUBJECT

proteins in polyacrylamide gels, improvement by gel miniaturiza. tion, 177,287 Avermectins binding sites, chemiluminescent assay, 177,291 Azafulvenium salt formed by indole-3-methanol coupling with p-dimethylaminocinnamaldehyde, in indole-3-methanol spectrophotometric assay, 183,172 Azobenzene arsonate binding sites in proteins and peptides, sequential allotment, 177, 183 Azobisnitriles photolytic homolysis, in molecular imprinting of amino acid derivatives at O’C, 177,144 EJ Bacteria Bacillus subtilis, cell lysis by dimethyl sulfoxide, in preparation of DNA, 176,464 Clostridium absonum, derived 7&hydroxysteroid dehydrogenase, in Auorometric assay for ursodeoxycholic acid in human serum,

179,341 Escherichia coli cell lysis by dimethyl sulfoxide, in preparation of DNA, 176,464 growth and assay of &galactosidase activity, semiautomated microtiter plate-based system, 181,40 inner and outer membranes separated by Sephacryl S-500 chromatography, effects of stressors, 179,202 plasmid DNA, preparation by sucrose-mediated detergent lysis, 176,344 purification and characterization of recombinant rat interferon y,

176,326 purification of plasmid expressed protein lacking functional assay systems, 181,336 ribosomal protein, estimation by product of integrated peak area and flow rate, 182,295 spectrophotometric indicator assay for amino acid decarboxylases, 181,59 vitamin Kz biosynthesis, HPLC assay of enzymes involved in osuccinylbenzoic acid synthesis, 178,306 Halobacterium hulobium, [2Hs]tryptophan-hacteriorhodopsin, deuterated tryptophan assay by HPLC-thermospray MS, 181,271 identification by nucleic acid hybridization after sample labeling,

177,85 lysis

and

DNA

hybridization,

assay

with

oligonucleotide

probe,

181,23 Staphylococcus aureus, plasmid DNA, preparation by sucrose-mediated detergent lysis, 176,344 Streptomyces aureofaciens, tetracycline biosynthesis, terminal reaction kinetics, analysis by direct-injection reverse-phase HPLC micromethod, 181,125 Synechococcus PCC 6301 and Synechocystis PCC 6803, ammonia assimilating enzymes, HPLC assay in ui@o and in situ, 180, 152 Thermoanaerobacter finnii, intracellular pyridine nucleotide levels, bioluminometric assay, 180,17 Zymomonas mobilis, assay for hopanoids by HPLC with uv detection, 181,120 Bacteriophage X cosmids carrying two cohesive end sites arrayed in tandem, construction and characterization, 176,117 gtll, DNA purification by hybridization size screening for isolation of longest length cDNA clones, 183,89 large-scale preparation by tangential flow ultrafiltration for isolation of DNA, 176,373 Bacteriorhodopsin [2H,]tryptophan-laheled, deuterated tryptophan assay by HPLCthermospray MS (Halobacterium halobium), 181,271

INDEX

Bathophenanthroline -Bu(II) complexes, application as nonradioactive labels in dideoxy DNA sequencing, 181,216 Benedict test quantitative, application of 2,2’-bicinchoninic acid, 182,54 Benzamidine immobilized on 3-isothiocyanatopropyltriethoxysilane-activated nonporous monodisperse silicas, affinity chromatography, 178, 171 Benzo[a]pyrene-4,5-oxide metabolizing cytosolic glutathione S-transferase and microsomal epoxide hydrolase, reverse-phase HPLC, 178,153 Benzyldimethyl-n-hexadecylammonium chloride and SDS, in 2-D gel electrophoretic purification of proteins, 178, 457; erratum, 180,194 Benzyl esters preparation from peptides by liquid secondary-ion mass spectrome-

try,182,165 Bicinchoninic acid in protein analysis in astrocyte monolayer cultures (rat brain), 177, 41 protein assay based on, elimination of interfering substances, 180, 136 in quantitative Benedict test, 182,54 Bile bilirubin conjugates, reverse-phase HPLC (dog), 183,269 Bile acids CoA:glycine/taurine N-acyltransferase, radioassay with n-hutanol solvent extraction (human liver), 182,360 inhibition of carnitine acetyltransferase, implications for carnitine analysis (rat), 179,262 Bilirubin conjugates, reverse-phase HPLC (canine bile), 183,269 Binding assay flow-injection, speed increase, role of substrates for peroxidase,

181,379 glutamate receptors, microbial methodological artifacts, 177,250 ligand-receptor, application of small volume equilibrium dialysis system, 176,157 parameters, evaluation of uncertainties by sum-of-squares profile and Monte Carlo method, 176,161 with rhodamine B-labeled thyroid hormone, artifacts: formation of high-molecular-weight aggregates in solution, 176,440 serine hydroxymethyltransferase (rat lens, liver), 180,120 solid-phase, with neoglycoenzymes, lectin detection (jack bean seeds), 182,447 Binding constants measurement by titration calorimeter, 179,131 Biochemical systems complex, algorithms for physicochemical parameters, 181,220 Bioluminescent assay with glucose-6-phosphate dehydrogenase, application to biotin and streptavidin detection, 180,43 intracellular pyridine nucleotide levels (Z’hermoanaerobacter @xii), 180,17 metabolic intermediates of lactose synthesis in milk and plasma (human), 176,449 Biomolecules immobilization by alumina-phosphate complexes, review, 180,l Biotin detection, application of bioluminescent assays with glucose-6phosphate dehydrogenase, 180,43 labeled proteoglycans, electrophoretic separation on minigels and blotting onto nylon membranes before and after enzymatic digestion (human aorta), 179,245 RNA probes with, sensitivity in detection of DNA targets, effects of derivatization extent, 182,309 -streptavidin interaction, inhibition by nonfat dried milk, 181,318

CUMULATIVE 5-(Biotinamido)pentylamine in calorimetric assay of cellular transglutaminase (human, rat), 182,170 Biotinidase in dried blood spots, assay with microtiter transfer plates: identification of biotinidase deficiency and heterozygosity (human),

179,371 Biotinylation deoxyuridine

5’-triphosphate,

in nonradioactive

labeling

of DNA,

179,77 RNA degree, estimation by gel retardation assay, 179,98 in DNA immunodetection: monoclonal antibody reactivity to DNA-RNA hybrids, 181,96 2’,7’-Biscarboxyethyl-5(6)-carboxyfluorescein in estimation of mitochondrial matrix pH (bovine heart), 178,348 Bis(l-methylethyl) phosphorofluoridate, see Isoflurophate Blood, see also Plasma, Serum acylcarnitines, assay by FAB/MS and liquid chromatography/MS (human), 180,331 bound selenomethionine, trace levels, indirect determination (human), 178,77 cyanide and thiocyanate, voltammetric assay in small samples (human), 181,190 dried filter paper spots, biotinidase activity, assay with microtiter transfer plates: identification of biotinidase deficiency and heterozygosity (human), 179,371 pantothenic acid, radiometric-microbiological assay (human), 178, 63 whole fluorine forms and distribution, analysis (human), 182,371 genomic DNA, purification with sodium perchlorate in place of phenol (human), 180,276 Blots peroxidase-stained, visualization, IOO-fold sensitivity enhancement with ultraviolet light, 182, 176 Blotting, see also specific types biotin-labeled electrophoresed proteoglycans onto nylon membranes before and after enzymatic digestion (human aorta),

179,245 on cationic nylon, in assay for radiolabeled glycosaminoglycans in crude cell extracts (mouse), 179,361 colony, in detection of specific RNA in eukaryotes, 176, 78 enzyme products, for nondestructive assay of catalytic function in polyacrylamide gels, 178,22 proteins, y-globulin-derived standards for determination of molecular weights, transfer, and immunodetection efficiencies, 176,

249 Bolton-Hunter reagent iodinated, catalytic activities and conjugation to proteins, measurement by iodide microassay, 179,138 Bone total cellular RNA, isolation and analysis (rat), 183,301 Book reviews Advances in Electrophoresis, Vol. 1. A. Chrambach et al. (Eds.), 1987,176,199 Advances in Electrophoresis, Vol. 2. A. Chrambach et al. (Eds.),

181,195 Analysis of Sterols and Other Biologically Significant Steroids. W. D. Nes and E. J. Parish (Eds.), 1989,183,325 Analytical Isotachophoresis. P. Bocek et al., 1988, 176,296 Basic Histochemistry. B. E. H. Sumner, 1988,178,432 Chemiluminescence. Principles and Applications in Biology and Medicine. A. K. Campbell, 1988,182,457 IBRO Handbook Series: Methods in the Neuroseiences, Vol. 11, Neuropeptides: A Methodology. G. Fink and J. Harmar (Ed.%), 1989,

182.458

SUBJECT

339

INDEX

Introduction

to Practical

Molecular

Biology.

P. D. Darbre,

1988,

180,396 Laboratory thetic

Techniques Polypeptides

in Biochemistry as Antigens.

M.

and Molecular Biology, H. V. VanRegenmortel

Synet

al.,1988,179,418 Methods in Cell Biology, Vols. 29 and 30, Fluorescence Microscopy of Living Cells in Culture, Parts A and B. Y. L. Wang and D. L. Taylor (Eds.), 1989,180,192 Methods in Enzymology, Vol. 143, Sulfur and Sulfur Amino Acids. W. B. Jakoby and 0. W. Griffith (Eds.), 1987,179,418 Methods in Enzymology, Vol. 156, Biomembranes, Part P, ATPDriven Pumps and Related Transport: The Na,K-Pump. S. Fleischer and B. Fleischer (Eds.), 1988,178,431 Methods in Enzymology, Vol. 157, Biomembranes, Part Q, ATPDriven Pumps and Related Transport: Calcium, Proton, and Potassium Pumps. S. Fleischer and B. Fleischer (Eds.), 1988, 178, 431 Methods in Enzymology, Vol. 159, initiation and Termination of Cyclic Nucleotide Action. J. D. Corbin and R. A. Johnson (Eds.), 1988,178,431 Methods in Enzymology, Vol. 161, Biomass, Part B, Lignin, Pectin, and Chitin. W. A. Wood and S. T. Kellogg (Eds.), 1988, 178,432 Methods in Enzymology, Vol. 162, Immunochemical Techniques, Part L, Chemotanis and Inflammation. G. Di Sabato (Ed.), 1988,

179,419 Methods in Enzymology, Vol. 167, Cyanobacteria. L. Packer and A. N. Glazer (Eds.), 1988,180,192 Methods in Enzymology, Vol. 168, Hormone Action, Part K, Neuroendocrine Peptides. P. M. Conn (Ed.), 1989, 182,458 Methods in Plant Molecular Biology. M. A. Schuler and R. E. Zielinski, 1989, 179,418 Neuromethods, Vol. 7, Lipids and Related Compounds. A. Boulton et al. (Eds.), 1988, 181,195 Neuromethods, Vol. 10, Analysis of Psychiatric Drugs. A. Boulton et al. (Eds.), 1988, 178,432 Recombinant DNA Laboratory Manual. J. W. Zyskind and S. I. Bernstein, 1989, 183,325 Scientists in Conference. V. Neuhoff, 1987, 176,199 Subcellular Fractionation and Centrifugation: A Strategic Approach. K. R. Smith and B. D. Hames, 180,193 Techniques in Cell Cycle Analysis. J. W. Gray and Z. Darzynkiewicz (Eds.), 180,396 Troubleshooting LC Systems--A Comprehensive Approach to Troubleshooting LC Equipment and Separations. J. W. Dolan and L. R. Snyder, 1989,182,457 Boronic acids for affinity chromatography: spectral methods for determinations of ionization and diol-binding constants, 178, 125 Boroxazolidones formation from a-amino acids, evaluation of stability, 178,276 Brain carboxypeptidase H, radiometric assay (rat), 178,27 3,4-dihydroxyphenylglycolaldehyde, HPLC detection (human),

180,79 excitatory amino acid receptors, autoradiographic analysis [3H]glutamate (rat), 177,150 extracts crude, identification of clathrin assembly protein AP180 dimensional SDS-PAGE (bovine), 179,352 deproteinized, enzymatic cycling assay for phenylpyruvate

with

L-

by two(rat),

183,210 small samples, S-adenosylmethionine assay by HPLC-electrochemistry (mouse), 181,331 forebrain proteins, isolation and sequence analysis with 2-D SDSPAGE coupled to high-pressure liquid extrusion (mouse), 180, 340 polyphosphoinositides, derivatization with 9-anthryldiazomethane, reverse-phase HPLC (rat), 179,127

340

CUMULATIVE

ribonuclease inhibitor, purification, characterization, and direct spectrophotometric assay (pig), 176,175 N-Bromoacetyl groups peptides with, automated synthesis, in preparation of synthetic peptide polymers, peptide-protein conjugates, and cyclic peptides,

177,373 5-Bromo-4-chloro-3-indolylphosphate -nitroblue tetrazolium, as chromogenic substrate in hepatitis B virus DNA hybridization assay, comparison with luminescent substrate, MO,95 5-Bromoindoxyl-@-D-cellobioside based assay for fungal cellulase in polyacrylamide gels: high sensitivity and resolution, 182,250 Buffers volatile, in ion-exchange chromatography of oligonucleotides, 178, 320 n-Butanol solvent extraction, in radioassay of bile acid CoA:glycine/taurine Nacyltransferase (human liver), 182,360 t-Butanol -water-ammonium sulfate, in three-phase partitioning isolation of cathepsin D (bovine spleen), 180,169 C Calcitonin immunodetection on nitrocellulose, 183,250 Calcium uptake arsenazo III-loaded liposomes, assay, 177,402 Calcium tartrate as adsorbent for gel chromatography, preparation and properties,

179,86 Calmodulin recombinant, metabolically %-labeled, in detection of calmodulinbinding proteins, 178,141 Capillary electrophoresis free-solution, peptide electrophoretic mobility in, semiempirical model, 179.28 -MS, and electrospray ionization-MS, in analysis of polypeptides and proteins, 179,404 Carbohydrates periodate oxidation analysis, application of iodate and periodate capillary zone electrophoresis, 177,62 reducing, as strong uv-absorbing and electrochemically sensitive 3methyl-1-phenyl-5pyrazolone derivatives, HPLC, 180,351 Carbonate dehydratase sequential SDS-PAGE and reverse-phase HPLC (bovine erythrocytes), 178,385 Carbon dioxide W-labeled, metabolically generated, microassay, application to ornithine decarboxylase measurement, 178,52 minimized escape from solution, effects on reaction rate linearity in vitamin K-dependent carboxylase assay, 182,207 Carbonic anhydrase, see Carbonate dehydratase Carbonyl groups in oxidatively modified proteins, determination by reduction with tritiated sodium borohydride, 177,419 Carboxylase vitamin K-dependent, assay accuracy: effects of minimized escape of COs from solution on reaction rate linearity, 182,207 Carboxylesterase active-site titrations, oxazolones as substrates, 181,321 Carboxylic acids porphyrin, and zinc protoporphyrin, simultaneous assay in urine by reverse-phase HPLC with fluorometry (human), 183,134 Carboxypeptidase A fluorescent oligopeptide energy transfer assay (bovine pancreas),

183,50

SUBJECT

INDEX

Carboxypeptidase H and other carboxypeptidase B-like enzymes, radiometric assay (rat brain, pancreas, pituitary), 178,27 Carboxypeptidase MI1 degradation of peptides, in C-terminal sequence determination with ‘%f plasma desorption mass spectrometry, 180,28 Carboxypeptidase Y, see Serine carboxypeptidase Carnitine free, and acylcarnitines, urinary levels, determination by FAB MS (human), 176,85 implications of carnitine acetyltransferase inhibition by bile acids (rat), 179,262 Carnitine acetyltransferase inhibition by bile acids, implications for carnitine analysis (rat),

179,262 Carnitine dehydrogenase in amplification of spectrophotometric

assay for NAD+

and NADH,

179,382 Cartilage articular, DNA, fluorophotometric assay with Hoechst 33258, 176, 128 total cellular RNA, isolation and analysis (rat), 183,301 Casein digestion, measurement by spectrofluorometry (bovine milk), 176, 239 Casein kinase II affinity purification with synthetic peptide substrates (rabbit muscle), 180,237 Catalase disk flotation assay, correlation with spectrophotometry (bovine liver), 178,404 in enzyme cycling assay for phenylpyruvate in tissue extracts (rat),

183,210 Catechol oxidase chromogenic assay based on addition of L-proline to quinones, 375 Cathepsin D detection wit.h self-quenched fluorogenic protein substrates, 261 isolation by three-phase partitioning in t-butanol/water/ammonium sulfate (bovine spleen), 180,169 labeled, immunoprecipitation with Eupergit ClZ acrylic

179, 176,

beads,

180,72 Cations effects on soluble and membrane-bound binding to liposomes, radiochemical

glutamate microassay

decarboxylase (rat brain),

182,64 Cell cultures astrocytes, protein analysis by bicinchoninic acid method (rat brain), 177,41 bacterial, growth and P-galactosidase assay, semiautomated microtiter plate-based system, 181,40 chromaffin cells, atria1 natriuretic factor, bidimensional reversephase HPLC (bovine), 179,24 erythroleukemia cells, small numbers, DNA and RNA simultaneous purification (human, mouse), 180,303 leukemia cells, large-scale synchronization with aphidicolin and study of cell cycle regulation of thymidylate synthase and dihydrofolate reductase (mouse), 182,338 mammalian microliter quantities, orthophosphate selective extraction, concentration, and calorimetric assay, 181,130 serum-free conditioned media, calorimetric assay of plasminogen activators and plasminogen activator inhibitors, 177,341 total Na+,K+-ATPase assay, 183,215 transfection by electroporation, optimization, 180,269 methylcellulose/agar suspension, NRK fibroblasts: direct biochemical analysis of anchorage-independent growth, 177,95

CUMULATIVE prostate carcinoma PC-3 cells, viable density, say, 181,149 suspension, device for aeration and mixing description, 182,151 various, sphingosine levels, enzymatic assay

chemiluminescent during (human,

NMR mouse),

SUBJECT asstudies,

183,

177 Cell cycle regulation of thymidylate synthase and dihydrofolate reductase after large-scale synchronization of cell cultures with aphidicolin (murine leukemia cells), 182,338 Cell lysates RNA, assay by noise-free molecular hybridization (human mononuclear cells), 181,371 Cell systems mammalian, viability assays, review, 179,l Cellulase continuous photometric assay with 4-methylumbelliferyl-~-D-cellobioside as substrate (Trichoderma reesei), 179,186 detection in polyacrylamide gels with 5-bromoindoxyl+D-cellobioside (Sporotrichum puluerulentum, Trichoderma spp.), 182, 250 fluorescence assay (Trichoderma uiride), 177,ll Cellulose acetate Titan III, based electrophoresis, with Auorometry, polyamines in urine and tissues (rat), 180,307 Cellulose nitrate recovery of active ribosomal complexes (Escherichia coli), 176,278 Centrifugation analytical, with preparative ultracentrifuges, review, 176,209 Percoll gradient, in isolation of distinct renal cortical cell populations for chemical cytotoxicity studies (rat), 182,271 sedimentation equilibrium, thermal convection in, stabilization by metrizamide, 178,198 Cephaloridine cell-type specific cytotoxicity (rat renal cortex), 182,271 Cerebroside sulfotransferase anion-exchange chromatography (rat kidney), 182,9 Cerebrospinal fluid small samples, S-adenosylmethionine assay by HPLC-electrochemistry (human), 181,331 Cesium thiocyanate -potassium thiocyanate high-resolution equilibrium gradients, in separation of density-labeled and unlabeled proteins, 177,333 Charge macromolecular, direct assay by equilibrium electrophoresis (equine heart), 182,377 Chemiluminescent assay avermectin binding sites, 177,291 in flow injection method for enzymatic assay of inorganic phosphate, 182,366 viable cell density of yeast, mammalian, and plant cells, 181, 149 Chemotaxis quantitation in vitro by computer-assisted scanning densitometer (rat fibroblasts, smooth muscle cells), 177, 72 Chitinase microbial, plant, or animal, uv detection after PAGE, 178,362 Chloramphenicol acetyltransferase in transfected cells, mixed-phase assay (frog, human), 179,19 transient gene expression assays, variation, role of transfection efficiency (mouse), 182,411 Chlorate ‘“Cl-labeled, production and purification by electrolytic oxidation and aqueous column chromatography, use in plant nitrate transport studies, 182,146 Chloride sensitive N-substituted quinoline compounds, synthesis and characterization as fluorescent indicators for biological applications, 178,355

INDEX

341

Chloroform and phenol in extraction of DNA in silica gel polymer-containing tubes, 183, 13 high-temperature mixtures, in preparation of RNA from unspheroplasted yeast cells, 176,303 I-Chloro-1-naphthol oxidative coupling to N,N’-dimethyl-p-phenylendiamine, in visualization of antigens on nitrocellulose, 183.9 l-p-Chlorophenyl-4,4-dimethyl-5-diethylamino-l-penten-3-one hydrobromide selective reactions with protein thiols and small-molecular-weight thiols, characterization, 177,237 Chloroplasts biogenesis, spectrofluorometric assay of monovinyl and divinyl chlorophyll(ide) a and b (corn, cucumber), 178,294 Chlorpyrifos active metabolite, hydrolysis by plasma paraoxonase/arylesterase, spectrophotometric assay (human), 180,242 3-(3-Cholamidylpropyl)-dimethylammonio-l-propanesulfonate in solubilization and partial purification of hepatic ol,-adrenergic receptors, role of glycerol, sodium phosphate, and sodium chloride (rat), 176,375 Cholecystokinin octapeptide sulfated, competitive ELISA, analysis, 183, 283 Cholesterol endogenous and exogenous [4-‘%I-labeled, as substrate in simultaneous reverse-phase HPLC assay ofcholesterol 7a-hydroxylase (rat liver), 182,212 side-chain cleavage by cytochrome P450,,,, HPLC assay, 182,327 Cholesterol 7ai-hydroxylase simultaneous reverse-phase HPLC assay with endogenous and exogenous [4-Wlcholesterol as substrate (rat liver), 182,212 Choline HPLC and GC-MS assays (human, rat), 180,85 in solutions and tissue extracts, assay by flow injection with immobilized enzymes and Auorometric or luminometric detection,

176,221 Cholinesterase semimicrotitration assay device (rat liver, plasma), 179,120 Choloyl CoA:glycine/taurine N-acyltransferase radioassay with n-butanol solvent extraction (human liver), 182, 360 Chromaffin granules adrenomedullary, ascorbic acid quantitation by HPLC with coulometric electrochemical detection (bovine), 181, 276 Chromatography, see also specific techniques agarose exclusion, low-percentage, in large-scale purification of plasmid insert DNA sequences, 179,167 aqueous column, in purification of 36C10; for plant nitrate transport studies, 182, 146 covalent, proteins, implications of salt-promoted thiophilic adsorp tion on sulfur-containing agarose, 176,330 fast protein liquid, plasmid DNA on Superose 6 preparative grade column, 177,378 gel permeation, double-stranded DNA, size-dependent fractionation: inverted elution order, 178,336 hydrophobic interaction, in alkaline pH, evaluation of salts for,

182,266 isocratic and gradient ion, in analysis of alkyl sulfates in protein solutions, 182,103 liquid-liquid partition, in aqueous two-phase systems, fractionation of immunoglobulins (human, rabbit), 183,305 metal ion-IDA Sepharose, serum proteins (human), 183,108 Sephacryl S-500, separated inner and outer membranes, effects of stressors (Escherichia coli), 179,202

342

CUMULATIVE

size-exclusion in analysis of ZO@hydroxysteroid dehydrogenase denaturation by urea and renaturation, 180,181 macromolecules, universal calibration of gel columns, 177,50 Chromogenic assay withparu-nitroaniline-derivatized peptide substrate, in detection of plasmin generated hy plasminogen activator immobilized on nitrocellulose, 177,78 Chromomycin A3 interaction with DNAs of different base composition and conformation, 179,401 Chromosomes metaphase, in situ hybridization of rRNA probes to, optimization (human), 182,25 Chymotrypsin detection with self-quenched fluorogenic protein substrates, 176, 261 a-Chymotrypsin active-site titrations, oxazolones as substrates, 181,321 carboxyl-terminal peptides, isolation by diagonal electrophoresis,

181,212 fluorescent oligopeptide energy transfer assay (bovine), 183,50 Circular dichroism in characterization of diastereoisomers of phosphate-ethylated dinucleoside monophosphates, 178,93 in determination of absolute configuration of sugar residues of complex polysaccharides, 179,209 protein spectral intensity, effect of protein adsorption onto glass surfaces, 177,178 Clathrin assembly protein AP180, identification in crude brain extracts by two-dimensional SDS-PAGE (bovine), 179,352 Cloning cosmids carrying two cohesive end sites of X phages arrayed in tandem, 176,117 Collagen mRNAs, base changes, detection and localization by chemical cleavage method (human, mouse), 183,263 nonisotopic evaluation by reverse-phase chromatography with flu.orometry (human fihroblasts), 176,395 polypeptide chains, peptide mapping: cyanogen bromide cleavage in polyacrylamide gels, 176,365 type I, peroxidase-labeled, in spectroscopic collagenase assay, 176, 150 Collagenase spectroscopic assay with peroxidase-labeled collagen (Clostridium histolyticum), 176,150 Colloidal gold in assay of antibodies eluted from nitrocellulose blots: application to electron microscopic immunocytochemistry, 183,225 stained Western-blotted nitrocellulose membranes, associated artifact appearance, 180,55 Colorimetry anchorage-dependent cells in tissue culture plates and on microcarriers, 181,209 with 5-(biotinamido)pentylamine, in cellular transglutaminase assay (human, rat), 182,170 free and bound L-fucose, 177,172 D-glucose consumption by cultured cells (bovine, human), 177,156 nitroblue tetrazolium-based, superoxide dismutase in tissue homogenates (rat mammary gland), 179,8 orthophosphate from microliter quantities of cultured mammalian cells, 181,130 with periodate-Schiff staining, in assay of mucus glycoproteins on nitrocellulose membranes, 182,160 plasma phenylalanine with L-phenylalanine dehydrogenase: application to monitoring phenylketonuria, 180,91

SUBJECT

INDEX

plasminogen activators and plasminogen activator inhibitors in serum-free cell culture-conditioned media, 177,341 pyrophosphate in presence of orthophosphate excess, application to mitochondrial pyrophosphate metabolism, 176,390 with solid alcohol oxidase, in enzymatic assay of ethanol and formaldehyde vapors, 182,109 Columns, see also specific types of columns gel-permeation HPLC, reverse-phase trace-enrichment loading (bovine, human pituitary), 179,222 Complement C3, peptide mapping after electroblotting onto polyvinylidene difluoride membranes from Tricine-SDS-PAGE (human),

181,33 Complex C recovery from

cellulose

nitrate

membranes

(Escherichia

coli),

176,

278 Computer applications control of pulsed-field gel electrophoresis at short switching intervals, 183,80 ISOTER program, analysis of TerTer enzyme kinetic mechanisms by simulation of isotope exchange at chemical equilibrium,

177,268 in least-squares method for determination of protein lecular ratio after amino acid analysis, 179,158 logarithmic timebase for stopped-flow data acquisition

conjugate

mo-

and analysis,

176,313 pre-steady-state solution to complex kinetic mechanisms, 176,427 quantitation of chemotaxis in vitro by computer-assisted scanning densitometer (rat fibroblasts, smooth muscle cells), 177, 72 Concanavalin A immobilized on 3-isothiocyanatopropyltriethoxysilane-activated nonporous monodisperse silicas, affinity chromatography, 179, 171 Condensing enzyme of microsomal fatty acid chain elongation system, spectrophotometric assay (rat), 179,251 Conjugation and simultaneous purification of antigenic peptides, synthesis strat-

egy,181,389 Convection thermal, in sedimentation equilibrium, stabilization by metrizamide, 178,198 Coomassie blue in assay of anchorage-dependent cells in tissue culture plates and on microcarriers, 181,209 protein assays assessment of nonlinearity, 179,198 detergent effects on sensitivity and variability, 178,263 refinement, 177,100 stained polyacrylamide gels preparation by one-step low-background procedure, 182,157 proteins Western blotted from, luminescent immunodetection,

179,206 Copper ionic,

interaction with peptides, evaluation by high-performance immobilized metal ion affinity chromatography, 183,159 quantitative Benedict test with 2,2’-bicinchoninic acid, 182,54 Copper chloride stained proteins on SDS-polyacrylamide gels, Western blotting,

180,311 Copper iodide staining of protein blots on nitrocellulose membranes, 181,250 Cosmids carrying two cohesive end sites of X phages arrayed in tandem, construction and characterization, 176,117 Cotinine idiotype-anti-idiotype immunoassay (human saliva), 182,187

CUMULATIVE

SUBJECT

Creatine kinase immobilized enzyme assay with amperometric detection, 176,234 microtiter plate assay with thio-NAD at 405 nm (murine myoblasts), 182,399 Crosslinkers heterobifunctional, in antibody immobilization on silica, 178,408 Crosslinking fibrinogen, inter- and intramolecularly after irradiation with visible light in presence of fluorescein, 179,154 glutaraldehyde with immobilized lectin columns, matrix stabilization, 177,168 Crystallins quantitative analysis by second derivative absorption spectroscopy (bovine lens), 181,79 Cyanide -naphthalenedialdehyde, precolumn derivatization of desmosine, isodesmosine, and other amino acids, 178,202 voltammetric assay in small biological samples (human blood, tissues), 181,190 Cyanogen bromide cleavage disulfide bond-containing polypeptides at methionine residues,

177,161 partial, proteins

in peptide mapping in polyacrylamide

of proteins gels: peptide

by SDS-PAGE, 178,391 mapping of collagen chains,

176,365 Cyanopropyl columns in HPLC separation of phospholipid classes and arachidonic acid (bovine, chicken, rat, soybean), 182,32 Cyclic nucleotides competitive ELISA, analysis, 183, 283 Cyclodextrin glucanotransferase, see Cyclomaltodextrin glucanotransferase Cyclodextrins and branched forms, reverse-phase HPLC with pulsed amperometric detection and membrane reactor, 179,336 Cyclomaltodextrin glucanotransferase cyclization activity, spectrophotometric assay (Bacillus subtilis),

181,6 Cystamine on solid supports, chemistry: definition of chemical events and ligand titers, 181, 242 Cysteine adducts of xenobiotics, structural analysis by FAB and tandem MS,

183,94 assay of SH groups

by electron

spin

resonance

(murine,

rat blood),

182,58 -cystine-containing proteins, reduction and fluorescent labeling prior to structural analyses, 180,231 plus half-cystine, determination in proteins after hydrochloric acid hydrolysis with disulfide compound as additive, 177,318 rich proteins, histochemical localization by tissue printing on nitrocellulose (barley), 182,334 Cystine -cysteine-containing proteins, reduction and fluorescent labeling prior to structural analyses, 180,231 half-, see Half-cystine Cytidine 2’-monophosphate binding to ribonuclease A active site, analysis with titration calorimeter, 179,131 Cytochrome c assay in testicular and somatic tissues from developing rats, 181, 140 charge, direct assay by equilibrium electrophoresis (equine heart),

182,377 sequential SDS-PAGE and 178,385 Cytochrome-c oxidase and pyruvate dehydrogenase,

reverse-phase

deficiencies,

HPLC

distinction

(equine

heart),

by determina-

INDEX

343

tion of (U-‘3C]glucose metabolism (human fibroblasts), 176, 382 Cytochrome oxidase, see Cytochrome-c oxidase Cytochrome P450r rates of de nouo synthesis, and steady-state levels, simultaneous assay by immunoblotting (chicken embryo hepatocytes), 179, 284 Cytochrome P450,, catalytic activity, assay by HPLC with uv absorption (bovine adrenal cortex), 182,327 Cytolysis and DNA hybridization in various bacteria, assay with oligonucleotide probe, 181,23 Cytoskeleton mitotic, preparation and characterization (Strongylocentrotus frunciscanus embryo), 181,51 Cytotoxicity cephaloridine, cell-type specificity (rat renal cortex), 182,271 D Data acquisition digital system for analytical ultracentrifuge, description, 180,319 Data analysis data from electrophoresis of gingival crevicular fluid, refinement method (human), 179,106 DEAE-cellulose bound W,N’“-methylene tetrahydrofolate, in assay for serine hydroxymethyltransferase (rat lens, liver), 180,120 filter disk, in assay for tRNA sulfurtransferase (Escherichia coli),

176,57 Decarboxylase and diaminopimelate epimerase, simultaneous assay by reversephase HPLC (Bacillus subtilis), 180,298 Deconjugation noradrenaline and dopamine in plasma, effects of ascorbic acid (human), 182,262 Dehydrogenases pyridine nucleotide-linked, staining and preparative electrophoresis in polyacrylamide gels, 178,243 Denaturation DNA, comparative effects of microwaves and boiling, 182,222 20@-hydroxysteroid dehydrogenase by urea, and renaturation, sizeexclusion chromatographic analysis, 180,181 Densitometry aldose reductase immunoquantitation in tissues (human), 176,66 dried polyacrylamide gels for, preparation, 179,242 scanning computer-assisted, in quantitation of chemotaxis in vitro (rat fibroblasts, smooth muscle cells), 177, 72 ultraviolet, in detection, quantitation, and preparative elution of protein bands in unstained gels, 176,400 Deoxygenation aqueous and organic solutions for transient studies, apparatus evaluation, 180,384 Deoxyribonuclease I genetic analysis by immunoblotting and zymogram method after isoelectric focusing (human urine), 183,84 and plasmin, simultaneous assay with radiolabeled human fibroblasts on microcarriers, 182,ZO Deoxyribonucleoproteins ultraviolet-irradiated, isolation of modified histone H3 by reversephase HPLC, 178,88 Deoxyribonucleoside triphosphates enzymatic assay with synthetic oligonucleotides as template primers, 180,222 Deoxyuridine 5’-triphosphate biotinylated, in nonradioactive labeling of DNA, 179,77

344

CUMULATIVE

in thymidylate synthase-inhibited cell extracts, radioimmunoassay (human lung carcinoma cells), 177,347 Derivative assay in analysis of fast components of DNA rejoining kinetics (human malignant glioma cells), 182,233 Derivatization ACV synthetase with o-phthaldialdehyde, for HPLC (Streptomyces clauuligerus), 178,399 amino acids with 1-fluoro-2,4-dinitrophenyl-5-L-alanine amide, subsequent analysis by reverse-phase HPLC, 17817 with 1-naphthylisocyanate for precolumn HPLC, 180,291 with o-phthalaldehyde and 9-fluorenylmethyl chloroformate, and fluorescence detection: application to protein structure determination, 178,227 cerebral polyphosphoinositides with 9-anthryldiazomethane, in reverse-phase HPLC (rat), 179,127 extent, effects on sensitivity of detection of DNA targets with biotinylated and fluoresceinated RNA probes, 182,309 y-glutamyl semialdehyde residues in oxidized proteins by Auoresceinamine, 182,226 hydrophilic peptides at picomole level by liquid secondary-ion mass spectrometry, 182,165 2-methyl5(4H)-thiazolones for HPLC, in thioacetylation method of protein sequencing, 181,113 nitrocellulose-bound proteins with Auorescein isothiocyanate, in situ method, 177,263 postcolumn, and ion-exchange chromatography, in amino acid analysis, 177,318 prechromatographic, with naphthalene-2,3dicarboxaldehyde, in assay of dopamine, norepinephrine, and related trace amines (human urine), 180,279 precolumn, desmosine, isodesmosine, and other amino acids with naphthalenedialdehyde/cyanide, 178,202 N-succinimidyl-2-(S)-methoxy-2-phenylacetic acid ester as aminedirected chiral reagent, suitability for enzymatic-scale resolutions, 178,177 trimethylsilyl, mononucleotides, effect on FAB MS detection of lownanogram amounts, 178,102 Desferrioxamine available iron, assay in tissues by reverse-phase HPLC with uv detection (rabbit), 180,126 Desmosine HPLC with electrochemical detection after precolumn derivatization with naphthalenedialdehyde/cyanide, 178,202 Detergents embryo lysis, in preparation and characterization of mitotic cytoskeleton, (Strongylocentrotusfranciscanus), 181,51 polyoxyethylene, nonionic, application in studies of phosphatidylinositol kinase, 179,66 DFP, see Isoflurophate Diacetyl spectrophotometric assay (rat), 180,349 Diacylglycerol kinase in sphingosine assay in cultured cells (human, mouse), 183,177 Dialysis drop, technique modification for larger volume samples, 179,75 equilibrium, small volume system, in protein-ligand binding assays,

176,157 4’,6-Diamidino-2-phenylindole -DNA complex, fluorescence assay, limitation in presence of excess tRNA, 176,416 interaction with DNAs of different base composition and conformation, 179,401 Diaminobenzoic acid fluorescence assay, with uv absorbance, in quantifying and ascertaining purity of DNA preparation (maize), 180,314

SUBJECT

INDEX

2,3-Diamino-2,3-dideoxy-D-glucuronic acid cation-exchange chromatography with fluorometric postcolumn derivatization, 176,63 1,6-Diamino-3,4-dihydroxyhexane on solid supports, chemistry: definition of chemical events and ligand titers, 181,242 meso-Diaminopimelate decarboxylase spectrophotometric assay (Escherichia coli), 181,297 Diaminopimelate epimerase and decarboxylase, simultaneous assay by reverse-phase HPLC (Bacillus subtilis), 180,298 tryptic peptides, amino acid analysis by derivatization and fluorescence detection, 178,227 Diastereoisomers phosphate-ethylated dinucleoside monophosphates, isolation and characterization, 178,93 Diethylenetriaminetetraacetic acid activated N-benzyl derivatives, synthesis and use as alternative reagents for antibody labeling with metal ions, 176,319 Digestion controlled partial restriction, DNA, by competitive methyltransferase/endonuclease reactions, 179,357 Dihydrofolate reductase binding to NADPH: logarithmic timebase for stopped-flow data acquisition and analysis, 176,313 cell cycle regulation after large-scale synchronization of cell cultures with aphidicolin (murine leukemia cells), 182,338 Dihydroneopterin triphosphate synthesis by GTP cyclohydrolase I repetitive recycling, 176,15 Dihydroorotase reverse-phase chromatography with radioactivity detection, 176, 105 Dihydropyridines PN200-110, binding to skeletal muscle membranes: characterization of ion channel activity (rabbit), 183,31 5,6-Dihydrouridine identification in normal human urine by GS-MS, 181,302 L-3,4-Dihydroxyphenylalanine in enzymatic-HPLC assay of plasma pyridoxal 5’phosphate (human), 181,267 3,4-Dihydroxyphenylglycolaldehyde cerebral, HPLC detection (human), 180,79 1,25-Dihydroxyvitamin Ds receptors immunoradiometric assay, 183,57 4,4-N,N-Dimethylaminoazobenzene-4’-isothiocyanate application to protein sequence analysis, improvements, 180,374 4-N,N-Dimethylaminoazobenzene 4’-thiohydantoin derivatized amino acids, HPLC analysis at sub-picomole levels: simultaneous manual sequencing of picomole quantities of several polypeptides, 177,46 p-Dimethylaminocinnamaldehyde in spectrophotometric assay of indole-3-methanol in small samples,

183,172 N,N’-Dimethyl-p-phenylendiamine oxidative coupling to 4-chloro-1-naphthol, in visualization of antigens on nitrocellulose, 183,9 5,5-Dimethyl-l-pyrroline-l-oxide spin trapping of oxygen-centered free radicals in biological systems, associated problems, review, 177,l Dimethyl sulfoxide bacterial cell lysis, in preparation of bacterial DNA, 176,464 2,4-Dinitrophenyl photoactivatable, labeling of DNA probes, evaluation, 177,392 3,4-Dinitrophenyl N-acetyl-fl-D-glucosaminide in spectrophotometric assay of N-acetyl-B-D-glucosaminidase or Nacetyl$D-hexosaminidase, 183,245

CUMULATIVE Dinucleoside monophosphates phosphate-ethylated, diastereoisomers, isolation tion, 17893 Dipeptidyl carboxypeptidase I fluorescent oligopeptide energy transfer assay

SUBJECT

and characteriza-

(human

kidney),

183,50 Diphenylamine reaction and flow cytometry, in quantitation of total DNA per cell in exponentially growing population (Trypanosoma cruzi), 177,353 l,l-Diphenylborinic acid reaction with a-amino acids: boroxazolidone formation and stability, 178,276 Disaccharides composition in heparin and heparan sulfate, analysis by ion-exchange chromatography, 176,96 unsaturated, from glycosaminoglycuronan, HPLC, 177,327 Disk flotation catalase, correlation with spectrophotometry (bovine liver), 178, 404 Dissociation constants enzyme-competitive inhibitor, experimental determination from displacement curves: application to human renin with fluorescence energy transfer to synthetic dansylated inhibitor peptide

181,172 Disulfide bonds polypeptides with, cyanogen bromide cleavage at methionine residues, 177,161 Disulfides as additive in hydrochloric acid hydrolysis of proteins, subsequent determination of cysteine plus half-cystine, 177,318 5,5’-Dithiobis(2-nitrobenzoic acid) in glutathione reductase assay with microplate reader, 183,195 Dithiothreitol interference in Lowry assay of proteins, elimination procedure,

183,275 Divinyl types

chlorophyll(ide) a and b, spectrofluorometric biogenesis (corn, cucumber),

assay, 178,294

in analysis

of chloroplast

DNA in articular cartilage, fluorophotometric assay with Hoechst 33258 (rabbit), 176,128 assays, application of guanine determination by HPLC with electrochemical detection (rat), 180,38 A-T content, determination by second-derivative ultraviolet spectroscopy, 176,265 bacterial, preparation by dimethyl sulfoxide-induced cell lysis, 176, 464 bacteriophage X double-stranded, size-dependent chromatographic fractionation: inverted elution order, 178,336 gtll, purification by hybridization size screening for isolation of longest length cDNA clones, 183,89 immobilization on nylon membranes and labeling by nick translation, 178,260 base composition and conformation, effect on dye spectrofluorometry, 179,401 binding proteins, and specific genomic DNA target sites, simultaneous characterization by South Western blot mapping (mouse), 179,299 cellular, t3H]thymidine labeling, in quantitation of anchorage-independent growth in suspension culture system (rat fibroblasts),

177,95 circular rapid

amplification

from

small

amounts

of unfractionated

RNA,

177,7 size estimation sis, 177,110

by y irradiation

and pulsed-field

gel electrophore-

INDEX

345

complementary protein S, site-specific mutagenesis by modification of Thermus aquaticus polymerase chain reaction (human), 180,147 synthesis, ethidium bromide-agarose plate assay, 178,269 controlled partial restriction digestions by competitive methyltransferase/endonuclease reactions, 179,357 denaturation, comparative effects of microwaves and boiling, 182, 222 -4’,6-diamidino-2-phenylindole complex, fluorescence assay, limitation in presence of excess tRNA, 176,416 extraction with phenol:chloroform, utilization of silica gel polymercontaining tubes, 183,13 fragmentation, assay with fiberglass filters, 183,42 fragments I-50-kilobase, pulsed-field gel electrophoresis techniques, 177, 282 produced by restriction enzymes, detection methods, 177,199 synthetic, placement of synthesized N7-ethyldeoxyguanosine 5’. triphosphate in specific sites, 179.60 gel electrophoresis, Lumpkin-DeJardin-Zimm model, extension and evaluation, 183,122 genomic, purification from whole blood with sodium perchlorate in place of phenol (human), 180,276 hepatitis B virus, hybridization assay, comparison of chemiluminescent and calorimetric substrates, 180,95 high-molecular-weight, agarose gel electrophoresis isolation, 181, 12 human immunodeficiency virus type 1, nonisotopic hybridization assay, 177,21 hybridization glucose-6-phosphate dehydrogenase as label for, 180,50 various bacteria, assay with oligonucleotide probe, 181,23 immunodetection with biotinylated probes: monoclonal antibody reactivity to DNA-RNA hybrids, 181,96 intercalated ethidium bromide on agarose gels, quantitative fluerescence, 181,197 y-irradiation-induced single-strand breaks, fast components of rejoining kinetics, derivative assay (human malignant glioma cells), 182,233 isolation from bacteriophage X prepared by tangential flow ultrafiltration,

176,373 from yeasts, 178,82 labeling nonradioactive method using biotin, 179,77 with photoactivatable hapten, evaluation, 177,392 large molecules, asymmetric field inversion gel electrophoresis,

178,172 mapping with photobiotin-labeled probes, cross-hybridization method, 178,273 plasmid fast protein liquid chromatography on Superose 6 preparative grade column, 177,378 insert sequences, large-scale purification by low-percentage agarose exclusion chromatography, 179,167 preparation from gram-positive and -negative bacteria by sucrose-mediated detergent lysis, 176,344 supercoiled, bidirectional sequencing, 183, 258 as template, and phosphorothioate-modified nucleotide, in oligonucleotide-directed mutagenesis technique, 179,309 primer-directed amplification with Thermus aquaticus-DNA polymerase, instrumentation, 176,33 probes in cell colony hybridization screening (murine fibroblasts), 179, 217 complementary to variable number tandem repeat locus, enzymatic synthesis (human), 179,280 radiolabeled, generation by polymerase chain reaction, 177,90

346

CUMULATIVE

SUBJECT

purification, solid-phase extraction procedure, 181,66 pyrimidine dimers, assay by HPLC/GC with electron capture detection, 182,424 quantity and purity of preparation, determination by diaminobenzoic acid fluorescence assay with uv absorbance (maize), 180, 314 restriction endonuclease co-digested fragments, discriminatory 32P 3’-end labeling, 1’77,413 -RNA hybrids enzyme immunoassay for nonisotopic detection of RNA, 181, 153 monoclonal antibodies to, in detection of unusual nucleic acids,

178,373 specific detection on nylon membranes by modified primer extension method, 179,366 and RNA, simultaneous purification from small numbers of tissue culture cells (human, mouse), 180,303 single-stranded affinity generation for dideoxy sequencing after polymerase chain reaction, 178,239 in gene assembly from partially overlapping fragments, 181,106 oligodeoxynucleotide-directed cleavage and repair: site-specific mutagenesis, 177,120 small molecules, hairpin structures, nondenaturing polyacrylamide gel electrophoresis, 183,152 solution-phase detection with interacting fluorescent labels and competitive hybridization, 183,231 targets, detection with biotinylated and Auoresceinated RNA probes: effects of derivatization extent on assay sensitivity,

182,309 total,

quantitation per cell in exponentially growing population, use of diphenylamine reaction and flow cytometry (T~panosoma cruzi), 177,353 very large molecules, separation by computer-controlled discontinuous rotating gel electrophoresis (yeast), 181,227 Z-, monoclonal antibodies to, in detection of unusual nucleic acids,

178,373 DNA methyltransferase -endonuclease competitive reactions, in controlled partial restriction DNA digestions, 179,357 DNA polymerase chain reaction, subsequent affinity generation of single-stranded DNA for dideoxy sequencing, 178,239 in enzymatic assay for deoxyribonucleoside triphosphates with synthetic oligonucleotides as template primers, 180,222 in primer-directed DNA amplification, instrumentation (Thermus

quaticw),176,33 DNase I, see Deoxyribonuclease DNA sequencing bidirectional, supercoiled dideoxy hathophenanthroline-Ru(II)

I plasmid

DNA,

183,258

complexes

as nonradioactive

labels,

181,216 after polymerase chain reaction, affinity stranded DNA for, 178,239 and footprinting in uiuo, review, 176,201 DNA topoisomerase fluorometric assays with low concentrations

generation

of ethidium

of single-

bromide,

182,284 Dopamine deconjugation in plasma, effect of ascorbic acid (human), 182,262 and related trace amines, assay by prechromatographic derivatization with naphthalene-2,3dicarboxaldehyde (human urine),

180,279 Dot assay human recombinant erythropoietin

erythropoietin as probe (murine

receptor, application spleen cells), 182,182

of lZ51-

INDEX

Dot-blot assay nonradioactive, protein-tyrosine kinase (human breast), 182,98 quantitative immunoenzyme, proteins on nitrocellulose, enhancement by SDS, 177,212 Dyes fluorescent, interaction with DNAs of different base composition and conformation, spectrofluorometric study, 179,401 E Ecdysone competitive ELISA, analysis, 183,283 Electroblotting myoglobin onto polyvinylidene difluoride membranes, subsequent gas-phase sequencing for assignment of correct molecular weights to markers (equine heart, sperm whale), 183,l peptides onto polyvinylidene difluoride membranes from TricineSDS-PAGE: application to human complement component C3,

181,33 proteins high-molecular-weight myofibrillar, onto nitrocellulose, affecting factors (bovine, rabbit), 180,205 onto nitrocellulose after fractionation on polyacrylamide gels, in visualization of acid phosphatase, 179,194 onto polyvinylidene difluoride membranes, subsequent amino acid analysis, 179,50 very-low-density and low-density, from composite gradient gels onto nylon membranes (human), 180,358 Electrochemical detection coulometric, with HPLC, ascorbic acid quantitation (bovine, human), 181,276 with enzymatic amplification, NAD+ and NADH, 179,382 glucose oxidase in plasma and tissues (mouse), 178,427 with HPLC in assay of small samples of S-adenosylmethionine in cerebrospinal fluid and brain extract (human, mouse), 181, 331 desmosine, isodesmosine, and other amino acids after precolumn derivatization with naphthalenedialdehyde/cyanide, 178,202 guanine, application to DNA and RNA assays (rat), 180,38 homocysteine and penicillamine and related symmetrical and mixed disulfides (human plasma, urine), 180,259 with liquid chromatography, endogenous platelet serotonin, in quantitation of paf-acether (rabbit), 182,419 Electroelution antigens immobilized on antibody-linked affinity matrices, 177, 314 Electron capture detection with HPLC/GC, DNA pyrimidine dimers, 182,424 Electron microscopy immunocytochemical, application of gold reagent assay of antibodies eluted from nitrocellulose blots, 183,225 Electron paramagnetic resonance low-temperature, in titration of oxygen-sensitive proteins, description of spectroelectrochemical cell, 181,283 Electron spin resonance assay of SH groups in low- and high-molecular-weight compounds (murine, rat blood), 182,58 Electrophoresis, see also specific techniques apparatus for concentration of dilute solutions of macromolecules, description, 182,315 capillary zone insulin and growth hormone (human), 177,20 reducing monosaccharides as borate complexes of N-S-pyridylglycamines, 176,72 simultaneous assay of iodate and periodate, application to carbohydrate analysis, 177,62 diagonal, carboxyl-terminal peptides from proteins, application to bacterial entomocidal toxin, 181,212

CUMULATIVE equilibrium,

direct

assay of macromolecular

charge

(equine

SUBJECT heart),

182,377 free-flow, hepatocyte plasma membrane domains (rat), 176,338 paper strip, radioactive assay of ATP-sulfurylase in crude extracts with sulfate or selenate as substrate, 176,294 Electroporation nonuniform electrical field, cylinder design (murine fibroblasts, leukemia cells), 182,253 for transfection of mammalian cell lines, optimization, 180,269 Electrospray ionization -MS, and capillary electrophoresis-MS, in analysis of polypeptides and proteins, 179,404 Electrotransfection parameters, testing with fluorescent dye Lucifer yellow CH (human cells), 181,309 ELISA, see Enzyme-linked immunosorbent assay Elongation factors EF-Tu, GTP analogs, bulk preparation (Escherichia coli), 177, 188 Embryo mitotic cytoskeleton, preparation and characterization (Strongylocentrotrcsframkcanu.s), 181,51 Endo-1.4~/3-glucanase, see Cellulase Endoglycosidases in characterization of glycoproteins and associated oligosaccharides, review, 180,195 Endonuclease fluorometric assays with low concentrations of ethidium bromide,

182,284 -methyh.ransferase competitive reactions, in controlled partial restriction DNA digestions, 179,357 P-Endorphin immunodetection on nitrocellulose, 183,250 Endothelial cells apical and luminal surfaces, selective radioiodination with iodogencoated Sephadex G-25, in vitro and in situ studies (bovine, rabbit aorta), 179,274 D-ghCose consumption in culture, calorimetric assay (bovine cornea), 177, 156 Energy transfer assay fluorescent oligopeptides, applications for neutral proteases, 183, 50 Enterokinase, see Enteropeptidase Enteropeptidase activation of trypsinogen, size-exclusion HPLC assay (bovine),

176,132 Enucleation erythrocytes in mixing chamber, in preparation of plasma membranes (chicken, trout), 182,71 Enzymatic assay mitochondrial tricarboxylate carrier (bovine liver), 177,323 Enzymatic cycling assay phenylpyruvate in deproteinized tissue extracts (rat brain, kidney, liver), 183,210 Enzyme immunoassay homogeneous, with surfactant reversed micellar systems, in analysis of thyroxine, 181,145 nonisotopic RNA detection with monoclonal antibody in DNARNA hybrids, 181,153 Enzyme-linked immunoblot assay discrimination of RAS ~21 species: technique for dissection of gene family expression, 180, 158 Enzyme-linked immunosorbent assay alcohol dehydrogenase class I, II, and III isozymes (human liver),

176,48 application to peptides, steroids, and asparagine-linked oligosaccharides mination of associated epitopes, endpoint, fructose 2,6-bisphosphate

cyclic nucleotides, from glycoproteins, 182,200 in 96-well plates,

183,283 in deter-

178,324

347

INDEX

in estimation of small numbers of cells (human), 177,259 guanine nucleotide-binding protein, 176,185 Enzyme-linked immunosorbent-inhibition assay hyaluronic acid in serum and synovial fluid (human), 178,367 Enzymes coupling, theory, practical aspects, review, 176,1 isolation by reversed micelles, review, 178,217 Ter-Ter kinetic mechanisms, analysis by computer simulation of isotope exchange at chemical equilibrium: development and application of ISOTER program, 177,268 Epidermal growth factor transferred to nylon membranes for Western blot analysis, increase of retention by glutaraldehyde fixation, 178,255 Epithelial cells crude extracts, radiolabeled glycosaminoglycans in, identification by cationic nylon blot assay (mouse), 179,361 Epitopes on asparagine-linked oligosaccharides of glycoproteins, determination, 182,200 and mRNAs, simultaneous localization: extracellular matrix gene expression by cultured cells (human), 178,184 Epoxide hydrolase microsomal, and cytosolic glutathione S-transferase, activities toward benzo[o]pyrene-4,5-oxide, reverse-phase HPLC, 178,153 Epoxyeicosatrienoic acid enantiomers, resolution by chiral-phase HPLC, 182,300 Erythrocytes enucleation in mixing chamber in preparation of plasma membranes (chicken, trout), 182,71 glutathione SH groups, assay by electron spin resonance (mouse, rat), 182,58 lipids, one-step extraction and rapid assay of fatty acids (human),

181,341 membranes,

preparation

by filtration

through

hollow-fiber

system,

179,190 “‘P-labeled, inositol phosphate production, analysis by ion-pair reverse-phase HPLC (human), 179,90 uroporphyrinogen-I synthase, in Ampholine pH gradient, preparative free solution isoelectric focusing (human), 182,217 Erythropoietin receptors dot assay with human recombinant ““I-erythropoietin (murine spleen cells), 182,182 Ethanol cold, in fractionation method for purification of plasma apolipoproteins A-I and A-II (human), 178,301 detection in urine by enzymatic assays with superabsorbent polymers (human), 182,257 effect on inner and outer membranes separated by Sephacryl S-500 chromatography (Es&erichia coli), 179,202 vapors, calorimetric enzymatic assay with solid alcohol oxidase, 182,109 Ethidium bromide -agarose plate assay, for nonradioactive detection of cDNA synthesis, 178,269 DNA-intercalated, Auorometric assay on agarose gels, 181,197 low levels in Auorometric assays for DNA topoisomerase and endonuclease, 182,284 O-Ethyl S-[2-]bis(l-methylethyl)amino]ethyl]methylphosphonothielate, see VX N7-Ethyldeoxyguanosine 5’-triphosphate synthesis and placement in specific site in DNA, 179,60 Ethylene glycol pretreated spinal cord stored at -7O”C, isolation of motoneuron cell bodies (frog, rat), 180,286 N7-Ethylguanine placement in specific site in synthetic DNA, 179,60 Eupergit ClZ beads, in immunoprecipitation of labeled antigens, 180,72

348

CUMULATIVE

Europium labeling of antibodies: synthesis of diethylenetriaminetetraacetic acid activated N-benzyl derivatives as alternative reagents,

176,319 Exons excision, in mapping introns, 183,64 Extinction coefficients proteins, calculation from amino acid sequence data, 182,319 Extraction DNA with phenokchloroform, utilization of silica gel polymer-containing tubes, 183,13 erythrocyte lipids by isopropanol, and rapid assay of fatty acid composition (human), 181,341 inositol phosphates from tissues, review, 183,199 NAD and NADH from rapidly sampled cardiac tissue by Karp and Klingenberg methods, comparison (dog), 182,304 plant nucleotides, in combination with pre-HPLC purification and HPLC analysis, procedural evaluation, 183,312 proteins from green plant tissues for two-dimensional electrophoresis, 177,33 solid-phase, in DNA purification, 181,66 solvent with n-butanol, in radioassay of bile acid CoA:glycine/taurine Nacyltransferase (human liver), 182,360 selective, orthophosphate from microliter quantities of cultured mammalian cells, 181, 130 Extrusion high-pressure liquid, with 2-D SDS-PAGE, in protein isolation and sequence analysis (murine forebrain), 180,340

F FAB/MS, see Fast atom bombardment mass spectrometry Fast atom bombardment mass spectrometry free and acylcarnitines in urine (human), 176,85 and liquid chromatography/MS, application to analysis of acylcarnitines in blood, tissue, and urine (human), 180,331 negative ion, native gangliosides, use of neutral matrix, 177,296 nucleotides, low-nanogram detection, 178,102 and tandem MS, in structural analysis of cysteine, N-acetylcysteine, and glutathione adducts of xenobiotics, 183,94 Fatty acids binding to ‘j3Ni, in assay for nanomole quantities of lipase, 179,413 composition, rapid assay on isopropanol-extracted lipids (human erythrocytes), 181,341 covalently bound to acylatedproteins immobilized on nitrocellulose, identification, 183,220 microsomal, chain elongation system, condensing enzyme activity, spectrophotometric assay (rat), 179,251 pyrene-labeled, acylated lipids with, HPLC, 178,166 Fermentation penicillin V, spectrophotometric assay of hydroxylated by-products: application to screening of mutant penicillin producer strains onagar, 179,288 FerriDye comparison with AuroDye and India ink staining of proteins in subnanogram and nanogram range, 182,44 Fiberglass filters in assay of DNA fragmentation, 183,42 Fibrinogen generation of stable protein-protein crosslinks after irradiation with visible light in presence of Auorescein (human plasma),

179,154 Fibrinolysin and DNase, simultaneous blasts on microcarriers, generated by plasminogen

assay with radiolabeled human fibro182,20 activator immobilized on nitrocellulose,

SUBJECT

INDEX

chromogenic assay with para-nitroaniline-derivatized peptide substrate, 177,78 Fibroblast growth factor basic, transferred to nylon membranes for Western blot analysis, increase of retention by glutaraldehyde fixation, 178,255 Fibroblasts anchorage-independent growth, quantitation in suspension culture system by radiolabeling of cellular DNA, 177,95 chemotaxis in u&o, quantitation by computer-assisted scanning densitometry (rat), 177,72 colony hybridization with DNA probes (mouse), 179,217 electroporation with nonuniform electrical field, cylinder design (mouse), 182,253 expression of extracellular matrix genes in u&o: simultaneous localization of mRNAs and antigenic epitopes (human), 178,184 D-&cOSe consumption in culture, calorimetric assay (bovine cornea), 177,156 lysosomal membrane-bound glucocerebrosidase, purification by gel permeation HPLC (human), 179,312 radiolabeled, on microcarriers, in simultaneous assay for plasmin and DNase (human), 182,20 small numbers, estimation by ELISA (human), 177,259 transfected, chloramphenicol acetyltransferase activity, mixedphase assay (Xenopus laeuis), 179,19 Fibronectin adsorption onto glass surfaces, effect on intensity of circular dichroism spectra (bovine plasma), 177,178 Filtration erythrocyte hemolysate mixture through hollow-fiber system, in preparation of membranes, 179,190 Firefly luciferase, see Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing) Fixation glutaraldehyde-mediated, increase of retention by low-molecularweight growth factors transferred to nylon membranes for Western blot analysis, 178,255 proteins and peptides on nitrocellulose, improvement of indii ink staining sensitivity, 174,97; erratum, 178,215 Flow cytometry and diphenylamine reaction, in quantitation of total DNA per cell in exponentially growing population (Trypanosoma cruzi),

177,353 fluorescein-methotrexate-based bioassay for plasma methotrexate and trimetrexate levels in cancer patients, 178,287 Flow injection analysis binding assays with, speed increase, role of substrates for peroxidase, 181,379 with immobilized enzymes and chemiluminescence detection, in assay of inorganic phosphate, 182,366 and fluorometric or luminometric detection, in assay of acetylcholine and choline in solutions and tissue extracts, 176,221 Flow radioactivity detection with HPLC fatty acids covalently bound to acylated proteins, 183,220 oligosaccharides in picomole amounts, 178,378 Flow rate and integrated peak area, product of, in protein estimation (Escherichiu coli), 182,295 9-Fluorenylmethyl chloroformate derivatized amino acids, high-sensitivity analysis with fluorescence detection: application to protein structure determination, 178, 227 Fluorescein conjugated methotrexate, in flow cytometric bioassay of plasma methotrexate and trimetrexate levels in cancer patients, 178, 287

CUMULATIVE

SUBJECT

hydrophilic derivatives, application as reagents for liposome immunolytic assays, 180,140 photosensitized fibrinogen irradiation reaction, generation of stable protein-protein crosslinks (human), 179,154 RNA probes with, sensitivity in detection of DNA targets, effects of derivatization extent, 182,309 Fluoresceinamine derivatization of y-glutamyl semialdehyde residues in oxidized proteins, 182, 226 Fluorescein isothiocyanate derivatization in situ, in detection of nitrocellulose-bound proteins,

177,263 Fluorescence 2’,7’-biscarboxyethyl-5(6)-carboxyfluorescein, in estimation of mitochondrial matrix pH (bovine heart), 178,348 diaminobenzoic acid, with uv absorbance, in assay for quantity and purity of DNA preparation (maize), 180,314 grade adenosine deaminase, production by laser photobleaching (bovine intestinal mucosa), 181,383 phycoerythrin, in assay for peroxy radicals: screen for biologically relevant protective agents, 177,300 quantitative, DNA-intercalated ethidium bromide on agarose gels,

181,197 Fluorescence energy transfer renin to synthetic dansylated inhibitor peptide, in application of assay for enzyme-competitive inhibitor dissociation constants from displacement curves (human), 181,172 Fluorescent labeling cyst(e)ine-containing proteins for structural analyses, 180,231 interacting labels, and competitive hybridization, in solution-phase detection of polynucleotides, 183,231 polypeptides in polyacrylamide gels for laser excitation, 179,291 Fluorine distribution and forms in whole blood and serum, analysis (human),

182,371 5-Fluoro-2’-deoxyuridine-5’-monophosphate FAB/MS, low-nanogram detection, 178,102 l-Fluoro-2,4-dinitrophenyl+L-alanine amide derivatized amino acids, analysis by reverse-phase HPLC, 178,17 Fluorography with sodium salicylate, tritium in thin-layer chromatograms and trocellulose blots, 178,327 Fluorometry alcohol dehydrogenase isozymes (human), 178,57 amyloid fibrils in vitro with thioflavine T (murine liver), 177,244 with bacterial 7&hydroxysteroid dehydrogenase, ursodeoxycholic acid in serum (human), 179,341 with cation-exchange chromatography, 2,3-diamino-2,3-dideoxy-oglucuronic acid, 176,63 chloride levels in biological samples: synthesis and characterization of sensitive indicators, 178,355 derivatized amino acids, high-sensitivity analysis: application protein structure determination, 178,227 DNA-4’,6-diamidino-2-phenylindole complex, assay limitation presence of excess tRNA, 176,416 with electrophoresis on Titan III cellulose acetate, polyamines urine and tissues (rat), 180,307 enzymes generating reducing sugars, 177, 11 and flow injection with immobilized enzymes, in assay of acetylcholine and choline, 176,221 glutathione after HPLC separation from GSH-orthophthalaldehyde adduct, 179,236 with HPLC N-acetylneuraminic acid and mono-O-acetylated derivatives sera (human, rat), 179, 162 hepatic folates (rat), 176,406 homocvsteine in olasma (human). 178.208

ni-

to in in

in

INDEX

349

in nonisotopic evaluation of collagen in fibroblast cultures (human), 176,395 physostigmine in plasma (human), 177,207 platelet-activating factor, 180,131 in simultaneous assay of zinc protoporphyrin and porphyrin carboxylic acids in urine (human), 183,134 with low concentrations of ethidium bromide, in DNA topoisomerase and endonuclease assays, 182,284 one- and two-step methods for assay of general aminotransferases with glutamate dehydrogenase (mouse, rat), 182,129 phospholipase A2 continuous assay with pyrene-labeled phospholipids in presence of serum albumin, 177,103 protease detection with self-quenched Auorogenic protein substrates, 176,261 qualitative assay of tyrosyl radical scavenging activity: ovothiol A identification as efficient scavenger, 180,326 in viability assay for studies of reactive drug intermediates, 177, 364 Fluorophotometry DNA in articular cartilage, assay with Hoechst 33258 (rabbit), 176, 128 Folates hepatic, reverse-phase chromatography with fluorometric detection (rat), 176,406 tissue, composition, analysis by affinity chromatography and ionpair HPLC (bovine, human, rat), 182,84 Footprinting in uiuo, and DNA sequencing, review, 176,201 Formaldehyde vapors, calorimetric enzymatic assay with solid alcohol oxidase,

182,109 Formate dehydrogenase in amplification of spectrophotometric and electrochemical assays for NAD+ and NADH, 179,382 Formycin triphosphate reaction with heavy meromyosin: logarithmic timebase for stoppedflow data acquisition and analysis, 176, 313 Fourier transform infrared spectroscopy diffuse reflectance, silica-immobilized N-hydroxysuccinimide active ester crosslinking agent and precursors, 176,137 liposomal lipids, 181,28 with microscopy, in assay of membrane lipids immobilized to silica: leaching and stability of bonded phases, 182,237 Fractionation immunoglobulins by liquid-liquid partition chromatography in aqueous two-phase systems (human, rabbit), 183,305 mitochondria with polycarbonate membrane filters (rat liver), 179, 114 plasma by cold ethanol method, in purification of apolipoproteins A-I and A-II (human), 178,301 Fragmentation DNA, assay with fiberglass filters, 183,42 Free radicals oxygen-centered, spin trapping in biological systems, associated problems, review, 177,l peroxy, phycoerythrin fluorescence-based assay: screen for biologically relevant protective agents, 177,300 Freeze-clamping prepared tissue samples, homogenization method of preparation for metabolite analyses (rat heart), 18 1,239 Freezing -7O”C, spinal cord after ethylene glycol treatment, subsequent isolation of motoneuron cell bodies (frog, rat), 180,286 Fructose 2,6-bisphosphate endpoint ELISA performed in 96-well plates, 178,324 L-Fucose free and bound, calorimetric assay, 177, 172 Fucosyltransferase in spermatogenic cells, TLC (mouse), 178,421

350

CUMULATIVE

Fungi Streptomyces clauuligerus, ACV synthetase after derivatization with o-phthaldialdehyde,

in cell extracts, 178,399

SUBJECT

HPLC

G GABA, see y-Aminobutyric acid &Galactosidase bacterial assay in semiautomated microtiter plate-based system, 181,40 chemical glycosylation for lectin solid-phase assays and glycohistochemistry (jack bean seeds, human carcinoma tissue), 182, 447 fluorescent-labeled, laser excitation in polyacrylamide gels, 179, 291 testicular, purification of fractions with high affinity for phosphomannosyl receptors (bovine), 182,432 Galactosylation glycoprotein variants, immunofluorometric assay with immobilized Bandeiraea simplicifolia II lectin, 177,383 Galactosyltransferase in spermatogenic cells, TLC (mouse), 178,421 Galacturonic acid oligomers from 3 to over 25 residues in length, ion-exchange chromatography, 178,248 Gamma irradiation in estimation of circular DNA size, 177, 110 Gangliosides native, FAB/MS with neutral matrix, 177,296 Ganglioside synthase anion-exchange chromatography (rat liver), 182,9 Gas chromatography with flame photometry, protein-associated sulfur (human, rat),

178,394 free amino acids as crystalline N-tert-butyloxycarbonyl 0-phenacyl derivatives (fruit fly), 176,368 and GC-MS, in characterization of permethylated oligosaccharides with molecular mass to 2300 (porcine intestinal mucosa), 182, 438 -HPLC, with electron capture detection, assay of DNA pyrimidine dimers, 182,424 inositol phosphates after ion-exchange chromatography, 176,109 Gas chromatography-mass spectrometry choline and choline metabolite levels in plasma and tissues (human, rat), 180,85 5,6-dihydrouridine in urine (human), 181,302 and GC, in characterization of permethylated oligosaccharides with molecular mass to 2300 (porcine intestinal mucosa), 182,438 negative ion chemical ionization, histamine and iV’-methylhistamine in plasma (dog, human), 178,414 negative ion, serum leukotriene B, assay (human), 179,304 Gases enzymatic analysis, calorimetric method: assay of ethanol and formaldehyde vapors with solid alcohol oxidase, 182,109 Gas-liquid systems computation of physicochemical parameters, algorithms, 181,220 Gas-phase sequencing myoglobin electroblotted on polyvinylidene difluoride, in assignment of correct molecular weights to markers (equine heart, sperm whale), 183,l GC-MS, see Gas chromatography-mass spectrometry Gel electrophoresis acylphosphatase on polyacrylamide, subsequent activity staining, in analysis of isozyme tissue distribution (chicken, pig), 183,46 apolipoprotein B species on SDS-agarose, 181,86 asymmetric field inversion, large DNA molecules, 178,172 chitinase on polyacrylamide, subsequent uv detection, 178,362

INDEX

clathrin assembly protein AP180 in crude brain extracts on twodimensional SDS-polyacrylamide (bovine), 179,352 computer-controlled discontinuous rotating, in separation of very large DNA molecules (yeast), 181,227 DNA high-molecular-weight, in agarose gels, 181,12 Lumpkin-DiJardin-Zimm model, extension and evaluation,

183,122 ethidium bromide, DNA-intercalated, on agarose gels, Auorometric analysis, 181,197 mini-two-dimensional, associated removal of gels from narrow glass tubes, 179,347 nondenaturing, hairpin structures of small DNAs on polyacrylamide, 183,152 peptides electroblotted onto polyvinylidene difluoride membranes, by Tricine-SDS-PAGE: application to human complement component C3,181,33 on SDS-polyacrylamide, micropreparative procedure for highsensitivity sequencing, review, 177,221 plasma membrane H+-ATPase on SDS-polyacrylamide (Neurosporu craw), 176,284 proteins on acetic acid/urea/Triton X-100, after two-dimensional gel electrophoresis, 177,388 gingival crevicular fluid, data refinement by clustering (human),

179,106 myofibrillar, high-molecular-weight, affecting factors (bovine, rabbit), on SDS-polyacrylamide micropreparative procedure for view, 177,221 subsequent peptide production, subsequent reverse-phase HPLC, proteoglycans on agarose-polyacrylamide with synovial fluid), 180,211 biotin-labeled, on SDS-polyacrylamide onto nylon membranes before

on SDS-polyacrylamide, 180,205 high-sensitivity

sequencing,

re-

178,233

178,385 triple

immunoblotting

(human

minigels, with blotting and after enzymatic digestion,

179,245 pulsed-field control at short switching intervals by microcomputer, 183,80 DNA fragments of 1 to 50 kilobases, various techniques, 177,282 in estimation of circular DNA size, 177,110 pyridine nucleotide-linked dehydrogenases on polyacrylamide,

178,243 thymic and nuclear extracts on ultrathin microgels, protein immunoblotting by unidirectional diffusion, 176,350 two-dimensional with high-pressure liquid extrusion, in protein isolation and sequence analysis (murine forebrain), 180,340 proteins extracted from green plant tissues, 177,33 plasma membrane, on SDS-polyacrylamide (Dictyostelium dkcoideum), 179,37 on polyacrylamide in presence of benzyldimethyl-n-hexadecylammonium chloride and sodium dodecyl sulfate, 176, 457; erratum, 180,194 and subsequent acetic acid/urea/Triton X-100 gel electrophoresis, 177,388 with SDS, actin-binding proteins, 178,32 Gel retardation assay in estimation of degree of RNA biotinylation, 179,98 Gels, see also specific gels calcium tartrate, preparation and chromatographic properties, 179, 86 composite gradient, electroblotting of low-density and very-lowdensitv linonroteins to nvlon membranes (human). 180.358

CUMULATIVE removal from narrow glass tubes phoresis, 179,347 in size-exclusion chromatography

in mini-two-dimensional columns,

universal

electrocalibration,

177,50 Genes ampicillin resistance, in chimeric plasmids of yeast cells: iodometric detection of fl-lactamase, 177,165 assembly from partially overlapping fragments with single-stranded DNA and sequence-specific synthetic oligodeoxynucleotides, 181,106 DNase I, polymorphism, analysis by immunohlotting and zymogram method after isoelectric focusing (human urine), 183,84 extracellular matrix, expression by cultured cells: simultaneous localization of mRNAs and antigenic epitopes (human), 178,184 prothymosin o, intron mapping by exon excision (human), 183,64 RAS, encoded p21 species, discrimination by enzyme-linked immunoblot assay: technique for dissection of gene family expression, 180,158 reporter, firetly luciferase, application to interleukin-2 gene promoter, 176,28 transient expression, enzymatic assays, variation (mouse), 182, 411 usg, hi.sT operon, plasmid-expressed product lacking functional assay systems, purification (Escherichia coli), 181,336 variable number tandem repeat locus, complementary DNA probes, enzymatic synthesis (human), 179,280 Gingival crevicular fluid electrophoresis data, refinement by clustering (human), 179,106 Glass protein adsorption, effect on intensity of circular dichroism spectra,

177,178 y-Globulin derived standards for determination of molecular weights, transfer, and immunodetection efficiencies in protein blotting procedures, 176,249 Glucagon adsorption onto glass surfaces, effect on intensity of CD spectra,

177,178 P-1,3-Glucanase isozymes, direct detection on polyacrylamide electrophoresis and isoelectrofocusing gels (tobacco), 182, 136 Glucocerebrosidase, see Glucosylceramidase Glucose bioluminescent microanalysis in milk and plasma (human), 176, 449 consumption by cultured cells, calorimetric assay (bovine, human),

177,156 radiometric assay, 180, 11 turnover detection of exogenous metabolites in presence of endogenous pools by multiple ‘%-labeling strategies and ‘sC NMR (human), 176,307 into various metabolite pools, determination for differential diagnosis of lactic acidemias (human fibroblasts), 176,382 Glucose dehydrogenase in amplification of spectrophotometric assay for NAD+ and NADH,

179,382 Glucose oxidase in assay for oxygen direct electrochemical

concentration quantitation

absolute value, 179, 124 in plasma and tissues (mouse),

178,427 Glucose l-phosphate bioluminescent microanalysis (human milk, plasma), 176,449 Glucose B-phosphate bioluminescent microanalysis (human milk, plasma), 178,449 Glucose-6-phosphate dehydrogenase application as label for nucleic acid hybridization reactions, 180, 50 in bioluminescent assays: application to hiotin and streptavidin detection, 180,43

SUBJECT

INDEX

351

coupled assay, for study of mammalian hexokinase regulation, 177, 396 a-Glucosidase type I, microplate assay (porcine kidney), 181,109 Glucosinolate biosynthesis, associated glucosyltransferase and sulfotransferase, radioassay (cabbage), 178,137 Glucosylceramidase lysosomal membrane-bound, purification from cultured fibroblasts by gel permeation HPLC (human), 179,312 Glucosyltransferase involved in glucosinolate biosynthesis, radioassay (cabbage), 178, 137 Glucuronides steroid, hydrolysis (equine urine), 182,289 L-Glutamate “H-labeled, autoradiographic analysis of excitatory amino acid receptors (rat brain), 17’7,150 Glutamate-ammonia ligase acid hydrolysis, in isolation of fluoresceinamine-derivatized oxidized y-glutamyl semialdehyde residue, 182,226 cyanobacterial, reverse-phase HPLC in oitro and in situ, 180,152 oxidatively modified, carbonyl groups, determination by reduction with tritiated sodium horohydride, 177,419 Glutamate decarboxylase ion-pair HPLC with fluorometry (Escherichiu coli), 179, 182 soluble and membrane-bound, binding to liposomes, radiochemical microassay: effects of cations, lipid composition, and pyridoxal 5’-phosphate (rat brain), 182,64 spectrophotometric indicator assay (Escherichia coli), 181,59 Glutamate dehydrogenase based method for assay of L-glutamic acid: formation of pyridine nucleotide fluorescent derivatives, 180,248 cyanohacterial, reverse-phase HPLC in vitro and in situ, 180,152 NADPH-linked, in spectrophotometric measurement of oxoglutarate and estimation of aminotransferase activities (Stichococcus bacillaris), 180,99 in one- and two-step fluorometry of aminotransferases (mouse, rat),

182,129 Glutamate receptors binding assays, microbial methodological artifacts, 177,250 Glutamate synthase cyanobacterial, reverse-phase HPLC in vitro and in situ, 180,152 L-Glutamic acid assay based on glutamate dehydrogenase: formation of pyridine nucleotide fluorescent derivatives, 180,248 Glutamine-phenylpyruvate aminotransferase in enzyme cycling assay for phenylpyruvate in tissue extracts (rat),

183,210 Glutamine synthetase, see Glutamate-ammonia ligase Glutamine transaminase K, see Glutamine-phenylpyruvate aminotransferase y-Glutamyl semialdehyde residues, derivatization in oxidized proteins by fluoresceinamine, 182,226 Glutaraldehyde fixation, increase of retention of low-molecular-weight growth factors transferred to nylon membranes for Western blot analysis,

178,255 in stabilization of immobilized lectin columns by crosslinking, 168 Glutathione adducts of xenobiotics, structural analysis by FAB and tandem

177, MS,

183,94 HPLC

with fluorometric detection of GSH-orthophthalaldehyde adduct, 179,236 monoesters, improved preparation methods, 183,16 monoethyl ester, HPLC analysis and direct preparation of free base form, 183,21

352

CUMULATIVE

SH groups, ESR assay (murine, rat erythrocytes), 182,58 Glutathione reductase (NAD(P)H) assay with 5,5’-dithiobis(2nitrobenzoic acid), application of microplate reader, 183,195 Glutathione S-transferase cytosolic, and microsomal epoxide hydrolase, activities toward benzo[a]pyrene-4,5-oxide, reverse-phase HPLC, 178,153 Glycerol luminometric assay application of linear plot for standard curves in kinetic substrate assays extended above Michaelis-Menten constant, 177,125 application to lipolysis studies (human adipose tissue), 177,132 as matrix in FAB/MS for native gangliosides, 177,296 radiometric assay, 180,ll role in solubilization and partial purification of a,-adrenergic receptors by CHAPS (rat liver), 176,375 Glycerol kinase immobilized on collagen membrane, in assay of creatine kinase with amperometric detection, 176,234 product blot for nondestructive assay of catalytic function in polyacrylamide gels, 17822 Glycerol-S-phosphate dehydrogenase in assay for picomole levels of phosphatidate (rat hepatocytes),

HO,24 Glycerol-3-phosphate oxidase immobilized on collagen membrane, in assay of creatine kinase with amperometric detection, 176,234 Glycerophosphocholine HPLC and GC-MS assay (human, rat), 180,&S Glycine hydroxymethyltransferase binding assay (rat lens, liver), 180,120 Glycogen NADH-linked luciferase assay: preparation of glycogen-free viable eccrine sweat glands (human), 176,228 radiometric assay, 180,ll Glycogen synthase R form in liver extract, assay (rat), 178,311 Glycogen synthase kinase-3 affinity purification with synthetic peptide substrates (rabbit muscle), 180,237 Glycohistochemistry ductal carcinoma tissue, application of neoglycoenzymes (human),

182,447 Glycoproteins and associated oligosaccharides, characterization with endoglycosidases, review, 180,195 derived asparagine-linked oligosaccharides, epitopes, determination, 182,200 detection with polyacrylic polyhydrazides, 181,182 galactosylation variants, immunofluorometric assay with immobilized Bandeiraea simplicifolia II lectin, 177,383 mucins, calorimetric assay with periodate-Schiff staining on nitrocellulose membranes, 182,160 variant surface, oligosaccharide components of glycosylphosphatidylinositol anchors, preparation and purification (Trypanosoma brucei), 181, 1 Glycosaminoglycans derived oligosaccharides, analysis by reverse-phase ion-pairing and ion-exchange HPLC with suppressed conductivity detection,

181,288 radiolabeled, identification in crude cell extracts by cationic nylon blots (mouse), 179,361 unsaturated disaccharides from, HPLC, 177,327 Glycosidase glycoprotein-processing, microplate assay (porcine kidney), 181,lO Glycosphingolipids underivatized, HPLC/atmospheric pressure ionization MS assay (bovine, human), 182,405

SUBJECT

INDEX

Glycosylphosphatidylinositol anchoring membrane proteins, oligosaccharide components, preparation and purification (Trypanosoma brucei), 181,l GMP (guanosine 5’-monophosphate) affinity chromatography with, for purification of mutant hypoxanthine phosphoribosyltransferase, 178, 148 Gold colloidal, see Colloidal gold Golgi apparatus membrane intravesicular contents, method for replacement with air-driven ultracentrifuge (rat liver), 180,216 Gradients Ampholine pH, uroporphyrinogen-I synthase in, preparative free solution isoelectric focusing (human erythrocytes), 182,217 high-resolution KSCN/CsSCN equilibrium, in separation of density-labeled and unlabeled proteins, 177,333 Growth hormone, see Somatotropin GTP (guanosine 5’-triphosphate) -elongation factor Tu analogs, bulk preparation (Escherichiu coli),

177,188 GTP cyclohydrolase repetitive recycling

I for synthesis

of dihydroneopterin

triphosphate,

176,15 Guanidine sulfate evaluation for hydrophobic interaction chromatography pH, 182,266 Guanidium -lithium chloride-based simultaneous purification RNA from small numbers of tissue culture mouse), 180,303 Guanine HPLC with electrochemical detection: application RNA assays (rat), 180,38 Guanine nucleotides binding protein G., competitive ELISA, 176,185

in alkaline

of DNA and cells (human,

to DNA

and

H Half-cystine plus cysteine, determination in proteins after hydrochloric acid hydrolysis with disulfide compound as additive, 177,318 Heart freeze-clamped tissue samples, homogenization procedure for metabolite analyses (rat), 181,239 NAD and NADH values in rapidly sampled tissues by Karp and Klingenberg extraction methods (dog), 182,304 Heat DNA denaturation by boiling, comparison with microwaves, 182, 222 measurement by titration calorimeter, 179,131 Heating time in sample buffer, effect on PAGE of high-molecular-weight myofibrillar proteins (bovine, rabbit), 180,205 Heat shock effect on inner and outer membranes separated by Sephacryl S-500 chromatography (Escherichia coli), 179,202 Hemoglobin methylated, assay by methyl ester hydrolysis to methanol (ferret, human), 179,326 R + T transition kinetics, measurement with stopped-flow frontface fluorometer, 176,19 Heparan sulfate disaccharide composition, analysis by ion-exchange chromatography, 176,96 Heparin disaccharide composition, analysis by ion-exchange chromatography, 176,96

CUMULATIVE

SUBJECT

Hepatitis DNA,

B virus hybridization assay, comparison of chemiluminescent and calorimetric substrates, 180,95 Hepatocytes, see also Liver cultured, pyridine dinucleotides, reverse-phase HPLC (rat), 179, 34 32P-labeled, inositol phosphate production, analysis by ion-pair reverse-phase HPLC, 179,90 plasma membrane domains, free-flow electrophoresis (rat), 176, 338 transfected, chloramphenicol acetyltransferase activity, mixedphase assay (human), 179,19 6-0-(N-Heptylcarbamoyl)-methyl-cY-~-glucopyranoside as surfactant for membrane studies, synthesis and characterization, 179,145 Hexokinase mammalian, regulation, study by two kinetic methods, 177,396 product blot for nondestructive assay of catalytic function in polyacrylamide gels, 17822 Hexoses estimation by phenol-sulfuric acid reaction, reevaluation, 181,lS High-performance liquid chromatography ACV synthetase derivatized with o-phthaldialdehyde (Streptomyces clauuligerus), 178,399 acylated lipids containing pyrene-labeled fatty acids, 178,166 in analysis of amino acids, precolumn derivatization with l-naphthylisocyanate, 180,291 4-N,N-dimethylaminoazobenzene 4’thiohydantoin amino acids at subpicomole levels: simultaneous manual sequencing of picomole quantities of several polypeptides, 177,46 glutathione monoethyl ester, 183,21 strictosidine synthase from plant cell cultures, 176,412 anion-exchange, see Ion-exchange chromatography with atmospheric pressure ionization MS, in assay of underivatized glycosphingolipids (bovine, human), 182,405 based semicontinuous assay for stromelysin, 180,110 chiral-phase, in resolution of epoxyeicosatrienoic acid enantiomers,

182,300 choline and choline metabolite levels in plasma and tissues rat), 180,85 derivatized 2-methyl-5(4H)-thiazolones, in thioacetylation of protein sequencing, 181,113 detection of 3,4-dihydroxyphenylglycolaldehyde (human

(human, method

INDEX

353

physostigmine (human plasma), 177,207 platelet-activating factor, 180,131 -gas chromatography, with electron capture detection, assay of DNA pyrimidine dimers, 182,424 gel permeation in purification of lysosomal membrane-bound glucocerebrosidase from cultured fibroblasts (human), 179,312 reverse-phase trace-enrichment loading of columns (bovine, human pituitary), 179,222 hydroperoxides, assay by iodometric technique, 176,360 inositol phosphates, review, 183,199 in one-step purification of monoclonal antibodies, thiophilic adsorbent for, 180,66 peptides and proteins, micropreparative procedure for high-sensitivity sequencing, review, 177,221 phospholipid classes and arachidonic acid on cyanopropyl columns (bovine, chicken, rat, soybean), 182,32 plant nucleotides, prior purification in combination with extraction and HPLC analysis, evaluation, 183,312 with postcolumn reaction system, assay of thiobarbituric acid-reactive substances in oxidized lipids (rat liver), 182,116 protein estimation by product of integrated peak area and flow rate (Escherichia coli), 182,295 with radiochemical assay, urate oxidase (human plasma), 176,290 reducing carbohydrates as strong uv-absorbing and electrochemitally sensitive 3-methyl-l-phenyl-5-pyrazolone derivatives,

180,351 reverse-phase, see Reverse-phase chromatography selenomethionine metabolites, 179,396 size-exclusion, in assay of enterokinase activation of trypsinogen (bovine), 176,132 subpicomole, and standard phenyl isothiocyanate reagent, in automated polypeptide sequencing, 183,290 supports made of membrane-forming lipids, synthesis and characterization, 176,36 thermospray, with MS deuterated tryptophan in [‘H&yptophan-bacteriorhodopsin (Halobacterium halobium), 181,271 in identification of phenylthiocarbamyl amino acids for compositional analysis, 176,269 oxidative warfarin metabolites, 177,307 two-step, myo-inositol and sorbitol in glomerular extract (rat kid-

ney),178,331 brain),

180,79 with electrochemical detection in assay of S-adenosylmethionine in small samples of brain extract and cerebrospinal fluid (human, mouse), 181,331 coulometric, in ascorbic acid quantitation (bovine, human), 181, 276 desmosine, isodesmosine, and other amino acids after precolumn derivatization with naphthalenedialdehyde/cyanide, 178,202 guanine: application to DNA and RNA assays (rat), 180,36 -enzymatic assay of plasma pyridoxal .Fphosphate (human), 181, 267 enzymatic-scale resolutions, suitability of N-succinimidyl-2-(S)methoxy-2-phenylacetic acid ester as amine-directed chiral derivatizing reagent, 178,177 enzymes involved in o-succinylbenzoic acid synthesis: analysis of vitamin K2 biosynthesis (Escherichia coli), 178,306 in presence of small amounts of product, 178,437 with flow radioactivity detection, oligosaccharides in picomole amounts, 178,378 with fluorometry N-acetylneuraminic acid and mono-0-acetylated derivatives (human, rat serum), 179,162 glutathione from GSH-orthophthalaldehyde adduct, 179,236 homocysteine (human plasma), 178,208

with ultraviolet absorption cytochrome P450,, activity, 182,327 hopanoids in bacterial cell cultures, 181,120 unsaturated disaccharides from glycosaminoglycuronan, 177,327 High-pressure liquid chromatography, see High-performance liquid chromatography Histamine plasma levels, GC-MS (dog, human), 178,414 Histidine methylation in actin, reverse-phase HPLC analysis (chicken, mammals), 178,194 residues, identification in proteins and peptides modified by arsanilit acid, 177,183 Histones H3, modified, isolation from uv-irradiated deoxyribonucleoprotein by reverse-phase HPLC, 178,68 Hoechst 33258 in fluorophotometric assay of articular cartilage DNA (rabbit), 176, 128 interaction with DNAs of different base composition and conformation, 179,401 Homocysteine HPLC with fluorescence detection (human plasma), 178,208 and related symmetrical and mixed disulfides, reverse-phase HPLC with electrochemical detection (human plasma, urine), 180, 259

354

CUMULATIVE

Homogenization freeze-clamped tissue samples for metabolite analyses, evaluation (rat heart), 181,239 unspheroplasted yeast cells in high-temperature phenol:chloroform mixtures, for RNA preparation, 176,303 Homolysis photolytic, azobisnitriles, in molecular imprinting of amino acid derivatives at O”C, 177,144 Hopanoids HPLC/uv detection assay (Zymomonas mobilis), 181,120 HPLC, see High-performance liquid chromatography Human immunodeficiency virus type 1, DNA, nonisotopic hybridization assay, 177,27 Hyaluronic acid enzyme-linked immunosorbent-inhibition assay (human serum, synovial fluid), 178,367 Hybridization in assay for DNA-RNA hybrids: DNA immunodetection with biotinylated RNAprobes, 181,96 hepatitis B virus DNA, comparison of chemiluminescent and colorimetric substrates, MO,95 cell colonies with DNA probes for screening (murine fibroblasts),

179,217 competitive, and interacting fluorescent labels, in solution-phase detection of polynucleotides, 183,231 cross-, with photobiotin-labeled probes, for DNA mapping, 178, 273 DNA by dot blots, glucose-6-phosphate dehydrogenase as label, 180, 50 from various bacteria, assay with oligonucleotide probe, 181,23 enzyme-nucleic acid probes, preparation with 5’-oligonucleotide hydrazide derivatives, 178,43 in isolation of recombinant bacteriophage h gt DNA, 183,89 noise-free molecular, for measurement of RNA in cell lysates (human leukocytes), 181,371 nonisotopic, in assay of human immunodeficiency virus type 1 DNA, 177,27 nucleic acids assays employing dA-tailed capture probes, multiple capture methods (Listerie monocytogenes), 181,345,360 in identification of microorganisms after labeling of test samples,

177,85 on nitrocellulose filters (Escherichiu coli), 182,280 in situ, rRNA probes to metaphase chromosomes, optimization (human), 182,25 Hydrocarbons content of silica-based bonded phases, Fourier transform infrared assay, 182,237 Hydrochloric acid hydrolysis associated hydroxyamino acid reactions, 181,315 of proteins with disulfide compound as additive, subsequent determination of cysteine plus half-cystine, 177,318 Hydrogen peroxide effect on inner and outer membranes separated by Sephacryl S-500 chromatography (Escherichia coli), 179,202 Hydrolases active-site titrations, oxazolones as substrates, 181,321 Hydrolysis acid and base, Immobilon-bound phosphoproteins, facilitation of phosphoamino acid analysis after gel fractionation, 176,22 chlorpyrifos and parathion active metabolites by plasma paraoxonase/arylesterase, spectrophotometric assays (human), 180, 242 hydrochloric acid, associated hydroxyamino acid reactions, 181, 315 limited, trypsin inhibitors, role in trypsin inhibition assay, 178,159

SUBJECT

INDEX

methyl esters to methanol, in assay of methylated hemoglobin (ferret, human), 179,326 one-step method for steroid sulfate and glucuronyl conjugates protL;r urine), 182,289 in hydrochloric acid, with disulfide compound as additive, subsequent determination of cysteine plus half-cystine, 177,318 and peptides in hermetically sealed microcapillary tube: high recovery of labile amino acids (bovine, chicken), 182,383 sulfoconjugated noradrenaline and dopamine in plasma, effects of ascorbic acid (human), 182,262 Hydroperoxides iodometric estimation by continuous-flow automated assay, 176, 353 spectrophotometric and HPLC assays by iodometric technique,

176,360 L-3-Hydroxyacyl-CoA dehydrogenase assay with substrates of different

chain

lengths

(porcine

heart),

180,105 Hydroxyamino acids reactions during hydrochloric acid hydrolysis, 181,315 4-Hydroxybenzoate 3-monooxygenase in assay for oxygen concentration absolute value, 179,124 8-Hydroxyquinoline -magnesium reaction, in measurement of stopped-flow fluorometer dead time, 181,234 fO/%Hydroxysteroid dehydrogenase denaturation by urea and renaturation, size-exclusion chromatographic analysis, 180,181 7&Hydroxysteroid dehydrogenase (NADP+) in fluorometric assay for ursodeoxycholic acid in human serum (Clostridium absonum), 179,341 N-Hydroxysuccinimide active ester, crosslinking agent and precursors, diffise reflectance infrared Fourier transform spectroscopy, 176,137 Hypoxanthine phosphoribosyltransferase mutant, GMP-affinity chromatography, 178,148

Idiotypes -anti-idiotype reaction, application to hapten immunoassay with cotinine (human saliva), 182,187 Immobilines polyacrylamide gels with, in preparative isoelectric focusing of leukoregulin, comparison with ampholine columns, 177,358 Immobilization biomolecules, alumina-phosphate complexes for, review, 180,l directional, Na,K-ATPase by wheat germ agglutinin linked to microtiter plates, 180,362 glycoprotein-derived asparagine-linked oligosaccharides on aminobonded high-performance TLC plate or poly-L-lysine, in determination of associated epitopes, 182,200 membrane lipids on silica, in Fourier transform infrared spectroscopic assay: leaching and stability of bonded phases, 182,237 proteins on oxidized crosslinked Sepharose preparations by reductive amination, 180,114 Immobilon bound phosphoproteins, acid/base hydrolysis, facilitation of phosphoamino acid analysis after gel fractionation, 176,22 Immunoassay, see also specific techniques generic liposome reagent, evaluation, 176,420 hapten, idiot-e-anti-idiotype, in cotinine assay (human saliva),

182,187 luminescence-based, Western-blotted proteins from Coomassie blue-stained polyacrylamide gels, 179,206 spot-blot, with affinity-purified antibodies, tyrosine hydroxylase (bovine, rat), 181,259

CUMULATIVE streptavidin-biotin, inhibition by nonfat dried milk, 181,318 with surface-enhanced Raman spectroscopy, in measurement of binding between biomolecules with mutual affinity, 182,388 Immunoblots apolipoprotein B species on SDS-agarose, 181,86 DNase I, after isoelectric focusing: enzyme genetic analysis (human urine), 183,84 improvement by chromatography of polyclonal antisera on keratin affinity columns (human, rat), 182,193 low-molecular-weight polypeptides on nitrocellulose, 183,250 in simultaneous assay of de nouo protein synthesis and steady-state levels (chicken embryo hepatocytes), 179,284 several antigens (rat brain), 177,203 thymus and nuclear extracts by unidirectional diffusion from ultrathin microgels, 176,350 triple, proteoglycan on nitrocellulose, and gel electrophoresis, resulting profiles (human synovial fluid), 180,2 11 with vertical slab gel focusing, in evaluation of protein phosphorylation state, 183,144 Immunocytochemistry electron microscopic, application of gold reagent assay of antibodies eluted from nitrocellulose blots, 183,225 Immunofluorometry with immobilized Bandeiraea simplicifolia II lectin, in determination of glycoprotein galactosylation variants, 177,383 Immunoglobulins IgA, IgG, and IgM, fractionation by liquid-liquid partition chromatography in aqueous two-phase systems (human), 183,305 IgG, eluted from nitrocellulose blots, assay with gold reagents: application to electron microscopic immunocytochemistry, 183, 225 Immunogold in assay of antibodies eluted from nitrocellulose blots: application to electron microscopic immunocytochemistry, 183,225 Immunolytic assay with liposomes, application of hydrophilic fluorescein derivatives as reagents, 180,140 Immunoprecipitation in measurement of 06-methylguanine and O’-methylthymine removal from oligodeoxynucleotides, 183, 74 solid-phase, labeled antigens with Eupergit Cl2 acrylic beads, 180,

72 Imprinting molecular, amino acid derivatives at 0°C with photolytic homolysis of azobisnitriles, 177, 144 India ink staining of proteins in nanogram and subnanogram range, comparison with AuroDye and FerriDye, 182,44 and peptides on nitrocellulose, sensitivity improvement by fixation, 174,97; erratum, 178, 215 Indole-3-methanol spectrophotometric assay in small samples with p-dimethylaminocinnamaldehyde, 183, 172 Initiation factors eIF-2, phosphorylation state, evaluation by combined vertical slab gel focusing and immunoblotting, 183,144 Inosine 3H-labeled, as substrate for purine-nucleoside phosphorylase isotopic assay, 183,279 Inositol benzoylated, reverse-phase HPLC, in assay of L-myo-inositol-lphosphate synthetase, 178,135 and sorbitol, in glomerular extract, two-step HPLC (rat kidney),

178,331 Inositol 1,2-cyclic partial chemical

4,5-trisphosphate synthesis, 183.26

SUBJECT

INDEX

355

Inositol phosphates analytical methods, review, 183,199 ion-exchange chromatography and GC, 176,109 32P-labeled, ion-pair reverse-phase HPLC: optimization of method and biological applications, 179,90 L-myo-Inositol-l-phosphate synthetase assay based on reverse-phase HPLC of benzoylated inositol, 178, 135 Inositol 1,4,5-trisphosphate levels in Dictyostelium discoideum, isotope dilution assay, 177, 115 Instrumentation for aeration and mixing of cell and organelle suspensions during NMR, description, 182,151 apparatus for primer-directed DNA amplification with Thermus aquaticus-DNA polymerase, 176,33 cation-exchange membrane reactor, with pulsed amperometric detection, in reverse-phase HPLC of cyclodextrins and branched forms, 179,336 chamber design for nonuniform electrical field electroporation,

182,253 cyclotherm apparatus for automated polymerase chain reaction, construction, 181,163 digital data acquisition system for analytical ultracentrifuge, 180, 319 electrophoresis apparatus computer-controlled discontinuous rotating, for separation of very large DNA molecules, 181, 227 for concentration of dilute solutions of macromolecules, description, 182,315 fiber-optic evanescent fluorosensor, acetylcholine receptor-based, characterization, 182,353 glass chamber for facilitation of gel removal from narrow glass tubes in mini-two-dimensional electrophoresis, 179,347 glass vessel for deoxygenation of solutions for transient studies,

180,384 hermetically sealed microcapillary tube for hydrolysis of proteins and peptides: high recovery of labile amino acids (bovine, chicken), 182,383 hollow-fiber filtration system for preparation of erythrocyte membranes, 179,190 incubation chamber for microassay of cellular secretory physiology: application to parietal cells (rabbit), 182,346 microplate-based system for semiautomated growth and assay of pgalactosidase activity of bacterial cells, 181,40 microplate reader, in assay of glutathione reductase with 5,5’-dithiobis(2-nitrobenzoic acid), 183,195 mixing chamber for enucleation of erythrocytes in preparation of plasma membranes (chicken, trout), 182,71 osmometer for analysis of aqueous biopolymer solutions, 176,217 pulse-switching device controlled by microcomputer, for pulsedfield gel electrophoresis at short switching intervals, 183,80 reverse-phase HPLC system combined with on-line trace enrichment, in analysis of cultured chromaffin cell atria1 natriuretic factor (bovine), 179,24 semimicrotitration device, in cholinesterase assay (rat liver, plasma), 179,120 spectroelectrochemical cell for low-temperature electron paramagnetic resonance titration of oxygen-sensitive proteins, description, 181,283 stopped-flow Auorometer dead time measurement by Mg”+ -8.hydroxyquinoline reaction,

181,234 front-face, for measurement of hemoglobin R + T transition kinetics, 176,19 superfusion system for measuring release of [3H]GABA from synaptosomes in vitro on subsecond time scale (rat brain), 178,8 titration calorimeter, for measurement of binding constants and heats, 179, 131

356

CUMULATIVE

SUBJECT

ultracentrifuge air-driven, in replacement of intravesicular contents of Golgi vesicles (rat liver), 180,216 preparative, application to analytical centrifugation, review, 178, 209 Insulin adsorption onto glass surfaces, effect on intensity of CD spectra,

branched

oligosaccharides

from

fetuin

(bovine),

inositol phosphates review, 183,199 subsequent GC assay, 176,109 and postcolumn derivatization, in amino acid analysis, 177,318 proteins on reversibly converted octadecyl-bonded silica column,

176,191

176,28 Introns mapping by exon excision, 1!33,64 Iodate andperiodate, simultaneous assay by capillary zone electrophoresis, application to carbohydrate analysis, 177,62 Iodide microassay, application to measurement of protein iodination and catalytic activities of iodo compounds, 179,138 N-Iodoacetyltyramine preparation and use in iZ51 labeling by alkylation of sulfhydryl groups, 179,389 Iodogen coated Sephadex G-25, in selective radioiodination of vascular endothelial cell apical and luminal surfaces in uitro and in situ (bovine, rabbit aorta), 179,274 Iodometry hydroperoxides continuous-flow automated assay, 176,353 spectrophotometric and HPLC assays, 176,360 &lactamase detection in yeast cells with ampicillin resistance gene in chimeric plasmids, 177,165 4-Iodophenol catalytic activities, measurement by iodide microassay, 179,138 Ion channels in skeletal muscle membranes, characterization by PN200-110 and ryanodine binding (rabbit), 183,31 Ion-exchange chromatography in analysis of disaccharide composition of heparin and heparan sulfate, 176, 96 plant nucleotides, in combination with extraction and pre-HPLC purification, procedural evaluation, 183,312 DEAE-cellulose, in assay for phosphatidylserine decarboxylase (yeast), 182,452 in denaturing solvents, in preparation of melittin depleted of phospholipase AZ, 178,118; erratum, 181,196 detection of myristoyl Co&protein N-myristoyltransferase activity on CM-Sepharose (murine brain), 181,254 with fluorometry, 2,3-diamino-2,3-dideoxy-D-glucuronic acid, 176, 63 galacturonic acid oligomers from 3 to over 25 residues in length,

178,248 synthase and cerebroside 182,9 with pulsed amperometric

sialylated

182,l

177,178 capillary zone electrophoresis (human), 177,!20 immunodetection on nitrocellulose, 183,250 Insulin-like growth factor I transferred to nylon membranes for Western blot analysis, increase of retention by glutaraldehyde fixation, 178,255 Interferon aA fusion with IFN--y: protein construction and phosphorylation (human), 178,342 Interferon y fusion with IFN-aA: protein construction and phosphorylation (human), 178,342 recombinant, purification from Escherichia coli and structural characterization (rat), 176,326 Interleukin 2 gene promoter, application of firefly luciferase as reporter gene,

ganglioside liver), high-pH,

INDEX

sulfotransferase detection,

(rat

kidney,

separation

of

in

purification of testicular &galactosidase fractions with high affinity for phosphomannosyl receptors (bovine), 182,432 purine precursors (murine L1210 cells), 182,121 on sulfoethyl aspartamide column, in peptide mapping of Staphylococcus aureus V6 protein digests, 176,255 with suppressed conductivity detection, and reverse-phase ion-pairing HPLC, in assay of glycosaminoglycan-derived oligosaccharides, 181,233 with volatile buffer system, oligonucleotides, 178,320 Ionization electrospray, see Electrospray ionization Iron desferrioxamine-available, assay in tissues by reverse-phase HPLC with uv detection (rabbit), 180,126 Irradiation fibrinogen with visible light in presence of fluorescein, generation of stable protein-protein crosslinks (human), 179,154 gamma, see Gamma irradiation Isodesmosine HPLC with electrochemical detection after precolumn derivatization with naphthalenedialdehyde/cyanide, 178,202 Isoelectric focusing agarose, with PhastSystem, plasma low- and very-low-density lipoproteins (human), 182,94 DNase I on polyacrylamide gel: enzyme genetic analysis by immunoblotting or zymogram method (human urine), 183,84 gels acidic, calibration with parvalbumins, 178, 121 direct detection of @-1,3-glucanase isozymes (tobacco), 182,136 vertical slab, with immunoblotting, in evaluation of protein phosphorylation state, 183,144 preparative free solution, erythrocyte uroporphyrinogen-I synthase in Ampholine pH gradient (human), 182,217 leukoregulin in ampholine columns and immobiline polyacrylamide gels, comparison, 177,358 Isoelectric point proteins, theoretical determination, 179,319 Isoflurophate low levels, microassay based on acetylcholinesterase inhibition,

180,380 Isoindoles derived from amino acids, o-phthalaldehyde, and N-acetyl-L-cysteine, formation and stability, kinetic-spectrophotometric study,

180,172 Isopropanol, see Isopropyl alcohol Isopropyl alcohol extraction of erythrocyte lipids allowing rapid assay of fatty acid composition (human), 181,341 3-Isothiocyanatopropyltriethoxysilane activated nonporous monodisperse silicas, with immobilized ligands, in affinity chromatography, 179,171 Isotope dilution assay inositol 1,4,5-trisphosphate levels (Dictyostehm discoideum), 177, 115 Isotope exchange at chemical equilibrium, simulation by ISOTER program in analysis of TerTer enzyme kinetic mechanisms, 177,268

CUMULATIVE Isotopic assay purine-nucleoside phosphorylase, strate and release of tritium, Ivermectin-luminol synthesis for chemiluminescent

based 183,279 assay

on [2-3H]inosine

of avermectin

binding

SUBJECT

as sub-

sites,

P-Lactamase in yeast cells with ampicillin resistance iodometric detection, 177,165 $Lactoglobulin amino acid analysis by derivatization

gene in chimeric

and fluorescence

plasmids,

detection,

178,227

177,291 K Keratin affinity columns, for chromatography of polyclonal antisera: improvement of immunoblot quality (human, rat), 182,193 Keratinocytes cultured, expression of extracellular matrix genes: simultaneous localization of mRNAs and antigenic epitopes (human), 178,184 Kidney bound selenomethionine, trace levels, indirect determination (pig), 178,77 cortex, distal and proximal tubular cells, isolation and efficacy in study of chemical-induced toxicity (rat), 182,271 deproteinized extracts, enzymatic cycling assay for phenylpyruvate (rat), 183,210 glomerular extract, myo-inositol and sorbitol, two-step HPLC (rat), 178,331 Kinetics complex mechanisms, general pre-steady-state solution with Laplace transforms, 176,427 isotope exchange, in TerTer enzyme system at chemical equilibrium, simulation by ISOTER program, 177,268 rejoining, DNA, analysis of fast components by derivative assay (human malignant glioma cells), 182,233 R -+ T transition, hemoglobin, measurement with stopped-flow front-face fluorometer, 176,19 -spectrophotometric studies on formation and stability of isoindoles derived from amino acids, o-phthalaldehyde, and N-acetyl-L-cysteine, 180, 172 terminal reactions of tetracycline biosynthesis, analysis by directinjection reverse-phase HPLC micromethod (Streptomyces aureofaciens), 181,125 L Labeling, see also specific techniques antibodies with metal ions: synthesis of diethylenetriaminetetraacetic acid activated N-benzyl derivatives as alternative reagents, 176,319 cell-surface, various techniques, in 2-D gel electrophoretic identification of plasma membrane proteins (Dictyostelium discoideum), 179,37 *%, multiple strategies, and ‘% NMR, in detection of exogenous metabolites in presence of endogenous pools: glucose turnover measurements (human), 176,307 DNA by nick translation after immobilization on nylon membranes,

178,260 probes with photoactivatable hapten, evaluation, 177,392 nonradioactive with bathophenanthroline-Ru(I1) complexes, application to dideoxy DNA sequencing, 181,216 DNA with biotinylated deoxyuridine 5’-triphosphate, 179,77 nucleic acids with glucose-6-phosphate dehydrogenase, for hybridization reactions, 180,50 photochemical, test sample, in identification of microorganisms by nucleic acid hybridization, 177,85 32P 3’ end of restriction endonuclease co-digested DNA fragments,

' 177,413 ?S recombinant ’ 141

357

INDEX

calmodulin,

for detection

of binding

proteins,

178,

hydrophobic interaction chromatography in alkaline pH, appropriate salts for, evaluation, 182,266 Lactose synthesis, metabolic intermediates, microanalysis in milk and plasma by bioluminescence (human), 176,449 Lanthanum chloride precipitation of nucleoside monophosphates, in assay for thymidine kinase, 178,38 Laser techniques excitation of fluorescent-labeled polypeptides in polyacrylamide gels, 179,291 Lavage fluid bronchoalveolar, oxidatively modified proteins, carbonyl group determination by reduction with tritiated sodium borohydride (dog, human), 177,419 Lectins Bandeiraea simplicifolia II, in immunofluorometric assay of glycoprotein galactosylation variants, 177,383 detection with neoglycoenzymes in solid-phase assays (jack bean seeds), 182,447 immobilized columns, stabilization by crosslinking with glutaraldehyde, 177,168 Leukocytes polymorphonuclear, see Neutrophils Leukoregulin biologically active, preparative isoelectric focusing in ampholine columns and immobiline polyacrylamide gels, comparison,

177,358 Leukotriene B, negative ion GC-MS assay (human serum), 179,304 Ligands binding to proteins, assay by small volume equilibrium dialysis system, 176,157 Linked enzyme assay double mixing technique, application to reaction kinetics, 176,444 Lipase, see Triacylglycerol lipase Lipids acylated, containing pyrene-labeled fatty acids, HPLC, 178,166 composition, effect on soluble and membrane-bound glutamate decarboxylase binding to liposomes, radiochemical microassay (rat brain), 182,64 liposomal, Fourier transform infrared spectroscopy, 181,28 membrane analysis by 500-MHz NMR (sea urchin), 178,67 composed chromatography supports, synthesis with lecithin and characterization, 176,36 silica-immobilized, Fourier transform infrared assay: leaching and stability of bonded phases, 182,237 one-step extraction and rapid fatty acid assay (human erythrocytes), 181,341 oxidized, thiobarbituric acid-reactive substances in, assay by HPLC with postcolumn reaction system (rat liver), 182,116 Lipolysis evaluation in adipose tissue, application of luminometric kinetic assay of glycerol (human), 177,132 Lipoproteins high-density, concentration of dilute solutions electrophoresis, description of apparatus (human plasma), 182,315 low-density and very-low-density agarose isoelectric focusing with PhastSystem (human plasma),

182,94

358

CUMULATIVE

SUBJECT

electroblotting from composite gradient gels to nylon membranes (human), 180,358 Liposomes arsenazo III-loaded, in quantitation of Car+ uptake, 177,402 associated lipids, Fourier transform infrared spectroscopy, 181,28 based immunolytic assays, application of hydrophilic fluorescein derivatives as reagents, 180,140 derivatized with antibodies, application as generic reagent for immunoassays, 176,420 glutamate decarboxylase binding, radiochemical microassay, effects of cations, lipid composition, and pyridoxal 5’-phosphate (rat brain), 182,64 Liquid chromatography derived fractions, detection of proteinase inhibitors, 179,349 with electrochemical detection, endogenous platelet serotonin, in quantitation of paf-acether (rabbit), 182,419 fast, androgen aromatase (human placenta), 181,167 -MS and FAB/MS, application to analysis of acylcarnitines in blood, tissue, and urine (human), 180,331 and reverse-phase HPLC, tocopherol and tocotrienol isomers, 180,

368 Lithium endogenous levels in serum and urine, electrothermal atomic absorption spectrophotometric assay: clinical applications (human), 182,245 Lithium chloride guanidium-based simultaneous purification of DNA and RNA from small numbers of tissue culture cells (human, mouse),

180,303 Lithium 3,5-diiodosalicylate catalytic activities, measurement by iodide microassay, 179,138 Liver, see also Hepatocytes acylcarnitines, assay by FAB/MS and liquid chromatography/MS (human), 180,331 a,-adrenergic receptors, solubilization and partial purification by CHAPS, role of glycerol, sodium phosphate, and sodium chloride (rat), 176,375 extracts deproteinized, enzymatic cycling assay for phenylpyruvate (rat),

183,210 glycogen synthase R assay (rat), 178,311 folates reverse-phase chromatography with fluorometric

detection

(rat),

176,406 tissue, composition, analysis by affinity chromatography and ionpair HPLC (rat), 182,84 pyruvate carboxylase and methylcrotonoyl-CoA carboxylase, identification by transblot method (rat), 177,57 Luciferase bacterial, NADH-linked, in glycogen assay: preparation of glycogen-free viable eccrine sweat glands (human), 176,228 firefly, see Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing) Lucifer yellow CH in testing for electrotransfection parameters (human cells), 181,

309 Luminometric assay with flow injection with immobilized enzymes, acetylcholine and choline, 176,221 glycerol application of linear plot for standard curves in kinetic substrate assays extended above Michaelis-Menten constant, 177, 125 in lipolysis studies (human adipose tissue), 177,132 Lymphoblastoid cells electrotransfection parameters, testing with Lucifer yellow CH (human), 181,309

INDEX

Lysine residues, identification in proteins and peptides modified by arsanilic acid, 177,183 Lysophosphatidylcholine HPLC and GC-MS (human, rat), 180,85 Lysoplasmalogenase in continuous spectrophotometric assay of phospholipase A2 hydrolyzing plasmalogens, 176,180 Lysozyme labeled, immunoprecipitation with Eupergit ClZ acrylic beads,

180,72 M Macromolecules charge, direct

assay by equilibrium

electrophoresis

(equine

heart),

182,377 dilute

solutions, electrophoretic concentration, description of apparatus (bovine, human), 182,315 size-exclusion chromatography, universal calibration of gel columns, 177,50 Magnesium -8-hydroxyquinoline reaction, in measurement of stopped-flow fluorometer dead time, 181,234 sensitivity of acetylcholinesterase molecular forms, in assay in skeletal muscle crude extracts (rat), 180,227 Magnetic separation pinocytic vesicles of defined age (Entamoebo histolytica), 182,77 Malondialdehyde free, in oxidized lipids, assay by HPLC with postcolumn reaction system (rat liver), 182,116 Mammary gland plasma membranes, isolation by two-phase polymer partitioning (rat), 177,194 superoxide dismutase activity in homogenates, nitroblue tetrazolium-based assay (rat), 179,8 Mapping techniques introns by exon excision, 183,64 tryptic digest of 23-kDa recombinant human growth hormone by plasma desorption MS, 183,190 Mass spectrometry atmospheric pressure ionization, with HPLC, in assay of underivatized glycosphingolipids (bovine, human), 182,405 with capillary electrophoresis and electrospray ionization, in analysis of polypeptides and proteins, 179,404 *‘rCf plasma desorption, peptides degraded with carboxypeptidases Y and MII, in C-terminal sequence determination, 180,28 fast atom bombardment, see Fast atom bombardment mass spectrometry liquid secondary-ion, in preparation of alkyl and benzyl esters from peptides at picomole level, 182,165 peptide synthesis by-products, 180,387 plasma desorption, in mapping of tryptic digest of 23-kDa recombinant human growth hormone, 183,190 tandem, and FAB/MS, in structural analysis of cysteine, N-acetylcysteine, and glutathione adducts of xenobiotics, 183,94 thermospray, with HPLC in assay of deuterated tryptophan in [‘H&ryptophan-bacteriorhodopsin (Halobacterium halobium), 181,271 in identification of phenylthiocarbamyl amino acids for compositional analysis, 176,269 oxidative warfarin metabolites, 177,307 Melittin depleted of phospholipase A*, preparation by ion-exchange chromatography in denaturing solvents, 178,118; erratum, 181,196 Membranes erythrocyte, preparation by filtration through hollow-fiber system,

179,190

CUMULATIVE hepatocyte,

domain

separation

by free-flow

electrophoresis

SUBJECT (rat),

176,338 immobilized artificial, for chromatography: supports composed of membrane lipids, 176,36 inner and outer, separated by Sephacryl S-500 chromatography, effects of stressors (Escherichia coli), 179,202 lipids analysis by 500 MHz NMR (sea urchin embryo), 17867 silica-immobilized, Fourier transform infrared assay: leaching and stability of bonded phases, 182,237 lysosomal, bound glucosylceramidase, purification from cultured fibroblasts by gel permeation HPLC (human), 179,312 mammary gland, isolation by two-phase polymer partitioning (rat),

177,194 preparation by erythrocyte enucleation in mixing chamber (chicken, trout), 182,71 proteins identification by cell surface labeling and 2-D PAGE (Dictyostelium discoideum), 179,37 surfactant 6-O-(N-heptylcarbamoyl)methyl-cu-~-glucopyranoside for analysis, synthesis and characterization, 179, 145 skeletal muscle, ion channel activity, characterization by PN200110 and ryanodine binding (rabbit), 183,31 2-Mercaptoethanol in upper reservoir buffer, effect on PAGE and electroblotting of high-molecular-weight myofibrillar proteins (bovine, rabbit),

180,205 Meromyosin heavy, reaction with formycin triphosphate: logarithmic timebase for stopped-flow data acquisition and analysis, 176,313 Metabolite analysis in freeze-clamped tissue, sample preparation method (rat heart), 181,239 Metal ions immobilized on stationary phases, in chromatographic separations of proteins (human serum), 183,108 Metal salts stained proteins on SDS-polyacrylamide gels, Western blots from,

180,311 Methanol release via methyl ester hydrolysis, in assay of methylated hemoglobin (ferret, human), 179,326 Methionine residues in polypeptides containing disulfide bonds, site of cyanogen bromide cleavage, 177,161 Methotrexate plasma levels in cancer patients, fluorescein-methotrexate-based flow cytometric bioassay, 178,287 4-Methoxy-1-naphthaldehyde as fluorogenic substrate in alcohol dehydrogenase fluorometric assay (human), 178,57 6-Methoxy-2-naphthaldehyde as fluorogenic substrate in alcohol dehydrogenase fluorometric assay (human), 178,57 Methylation histidine in actin, reverse-phase HPLC analysis (chicken, mammals), 178,194 reductive, in isotopic labeling of antibiotics, 181,90 Methylcrotonoyl-CoA carboxylase identification by transblot method (rat liver), 177,57 N5,Nio-Methylene tetrahydrofolate binding to DEAE-cellulose, in assay for serine hydroxymethyltransferase (rat lens, liver), 180,120 Methyl esters hydrolysis to methanol, in assay of methylated hemoglobin (ferret, human), 179,326

359

INDEX

Oe-Methylguanine removal from oligodeoxynucleotides, measurement by immunoprecipitation, 183,74 N’-Methylhistamine plasma levels, GC-MS (dog, human), 178,414 N-Methylphenazonium methosulfate and NADH, mediated reduction of nitroblue tetrazolium chloride, role of superoxide anions, 176,170 3-Methyl-1-phenyll5pyrazolone derivatives of reducing carbohydrates, strong uv-absorbing and electrochemically sensitive, HPLC, 180,351 04-Methylthymine removal from oligodeoxynucleotides, measurement by immunoprecipitation, 183,74 4-Methylumbelliferyl-/3-D-cellobioside as substrate in continuous photometric assay of cellulase (Trichoderma reesei), 179, 186 Metrizamide stabilization against convection in sedimentation equilibrium, 178,

198 Micelles reversed in homogeneous enzyme immunoassay for thyroxine, 181,145 in isolation of enzymes, review, 178,217 Michaelis-Menten constant kinetic substrate assays extended above, linear plot for standard curves: application to luminometric assay of glycerol, 177,125 Microassay cellular secretory physiology: application to parietal cells (rabbit),

182,346 ‘%O,,

metabolically generated, application to ornithine decarboxylase measurement, 178,52 iodide, application to measurement of protein iodination and catalytic activities of iodo compounds, 179,138 radiochemical, soluble and membrane-bound glutamate decarboxylase binding to liposomes, effects of cations, lipid composition, and pyridoxal5’-phosphate (rat brain), 182,64 Microbiological assay with radiometric assay, pantothenic acid in blood and milk (human), 178,63 Microcapillary tube hermetically sealed, for hydrolysis of proteins and peptides: high recovery of labile amino acids (bovine, chicken), 182,383 Microscopy interfaced with Fourier transform infrared spectrometer, in assay of silica-immobilized membrane lipids, 182, 237 Microsomes fatty acid chain elongation system, condensing enzyme activity, spectrophotometric assay (rat), 179,251 oxidized, thiobarbituric acid-reactive substances in, assay by HPLC with postcolumn reaction system (rat liver), 182,116 Microtiter assay glutathione reductase with 5,5’-dithiobis(2-nitrobenzoic acid), application of microplate reader, 183,195 microtiter plate-based adaptation of Nelson-Somogyi reducing-sugar assay, 182,192 biotinidase activity in dried blood spots: identification of biotinidase deficiency and heterozygosity (human), 179,371 for glycoprotein-processing glycosidases (porcine kidney), 181, 109 with thio-NAD, creatine kinase at 405 nm (murine myoblasts),

182,399 semiautomated, P-galactosidase activity of bacterial Microtiter plates 96-well in cell quantification with crystal violet staining 16

cells,

181,40

(human),

182,

360

CUMULATIVE

SUBJECT

covalently linked wheat germ agglutinin, directional immobilization of Na,K-ATPase, 180,362 Microwaves DNA denaturation, comparison with boiling, 182,222 Milk folate composition, analysis by affinity chromatography and ionpair HPLC (bovine, human), 182,84 metabolic intermediates of lactose synthesis, bioluminescent microanalysis (human), 176,449 nonfat dried, inhibition of streptavidin-biotin interaction, 181,318 pantothenic acid, radiometric-microbiological assay (human), 178, 63 Mithramycin interaction with DNAs of different base composition and conformation, 179,401 Mitochondria fractionation and analysis with polycarbonate membrane filters (rat liver), 179,114 matrix pH estimation with fluorescent probe (bovine heart), 178, 348 pyrophosphate metabolism, application of calorimetric assay for pyrophosphate in presence of orthophosphate excess (rat liver),

176,390 Mixed-phase assay chloramphenicol acetyltransferase in transfected eukaryotic cells (human, Xenopus laeuis), 179,19 Models computation of physicochemical parameters, in complex biochemical and gas-liquid systems, algorithms, 181,220 Lumpkin-DbJardin-Zimm, for DNA gel electrophoresis, extension and evaluation, 183,122 semiempirical, peptide electrophoretic mobility in free-solution capillary electrophoresis, 179,28 Molecular weight myoglobin markers, assignment by gas-phase sequencing after electroblotting on polyvinylidene difluoride (equine heart, sperm whale), 183,l protein, y-globulin-derived standards for determination in protein blotting procedures, 176,249 Monoclonal antibodies in detection of low-molecular-weight polypeptides on nitrocellulose,

183,250 to DNA-RNA in enzyme 153 reactivity:

hybrids immunoassay DNA

for nonisotopic

immunodetection

with

detection biotinylated

of RNA, RNA

18 1, probes,

181,96 and triplexes, poly(ADP-ribose), and Z-DNA, detection of unusual nucleic acids, 178,373 idiotype-anti-idiotype reaction, application to hapten immunoassay for cotinine (human saliva), 182,187 one-step HPLC purification, thiophilic adsorbent for, 180,66 Monoesters glutathione, improved preparation methods, 183,16 Mononuclear cells RNA in cell lysates, noise-free molecular hybridization (human),

181,371 Monosaccharides reducing, simultaneous assay as borate complexes of N-Z-pyridylglycamines by capillary zone electrophoresis, 176,72 Monosodium aspatiate evaluation for hydrophobic interaction chromatography in alkaline pH, 182,266 Monosodium glutamate evaluation for hydrophobic interaction chromatography in alkaline pH, 182,266

INDEX

Monovinyl chlorophyll(ide) types a and b, spectrofluorometric assay, in analysis of chloroplast biogenesis (corn, cucumber), 178,294 Mucosa intestinal, oligosaccharides with molecular mass to 2300, characterization by GC and GC-MS (swine), 182,438 Mucus calorimetric assay with periodate-Schiff staining on nitrocellulose membranes, 182,160 Muscles acylcarnitines, assay by FAB/MS and liquid chromatography/MS (human), 180,331 skeletal acetylcholinesterase molecular forms, estimation in crude extracts (rat), 180,227 membrane fractions, ion channel activity, characterization by PN200-110 and ryanodine binding (rabbit), 183,31 smooth, cell chemotaxis in vitro, quantitation by computer-assisted scanning densitometry (rat), 177,72 Mutagenesis oligonucleotide-directed, technique with plasmid DNA template and phosphorothioate-modified nucleotide, 179,309 site-specific general method involving modification of Thermus aqua&us polymerase chain reaction, 180,147 oligodeoxynucleotide-directed cleavage and repair of singlestranded vector, 177,120 Mutants penicillin producer strains, screening on agar, application of spectrophotometric assay of hydroxylated by-products of penicillin V fermentations, 179,288 Mutations point, in mRNAs, detection and localization by chemical cleavage method (human, mouse, rat), 183,263 Myelin central and peripheral nervous system, reverse-phase HPLC (rat), 180,59 Myeloperoxidase labeled, immunoprecipitation with Eupergit ClZ acrylic beads,

180,72 Myoblasts microtiter plate creatine kinase assay at 405 nm (mouse), 182,399 Myocytes total Na+,K+-ATPase, assay (rat), 183,215 Myofibrils associated high-molecular-weight proteins, factors affecting PAGE and electroblotting (bovine, rabbit), 180,205 Myoglobin electroblotted on polyvinylidene difluoride, assignment of correct molecular weights to markers by gas-phase sequencing (equine heart, sperm whale), 183,l sequential SDS-PAGE and reverse-phase HPLC (equine muscle),

178,385 Myristoyl CoA:protein N-myristoyltransferase detection by ion-exchange chromatography rine brain), 181,254

on CM-Sepharose

(mu-

N NAD (nicotinamide adenine dinucleotide) thioamido derivative, in microtiter plate creatine kinase assay at 405 nm (murine myoblasts), 182,399 values in rapidly sampled heart tissues by different extraction methods (dog), 182,304 NAD’ (oxidized nicotinamide adenine dinucleotide) and NADH combined levels, spectrophotometric and electrochemical assays, enzymatic amplification for, 179,382 NAD+ glycohydrolase, see NAD+ nucleosidase

CUMULATIVE

SUBJECT

NADH (reduced nicotinamide adenine dinucleotide) and NAD+ combined levels, spectrophotometric and electrochemical assays, enzymatic amplification for, 179,382 and phenazine methosulfate, mediated reduction of nitroblue tetrazolium chloride, role of superoxide anions, 176,170 values in rapidly sampled heart tissues by different extraction methods (dog), 182,304 NAD+ nucleosidase HPLC assay in presence of small amounts of product, 176,437 NAD(P)+ (oxidized nicotinamide adenine dinucleotide phosphate) intracellular levels, bioluminometric assay (Thermoanaerobacter

finnii),

180,17

NADPH (reduced nicotinamide adenine dinucleotide phosphate) binding to dihydrofolate reductase: logarithmic timebase stopped-flow data acquisition and analysis, 176,313 intracellular levels, bioluminometric assay (Thermoanaerobacter

for

finnii),180,17 linked

glutamate dehydrogenase, in spectrophotometric measurement of oxoglutarate and estimation of aminotransferase activities (Stichococclcs bacillaris), 180,SS Naphthalenedialdehyde -cyanide, precolumn derivatization of desmosine, isodesmosine, and other amino acids, 178,202 Naphthalene-2,3-dicarboxaldehyde in prechromatographic derivatization for assay of dopamine, norepinephrine, and related trace amines (human urine), 180,279 1-Naphthylisocyanate in precolumn HPLC derivatization for amino acid analysis, 180, 291 NelsonSomogyi assay for reducing sugars, adaptation to microtiter plate-based microassay, 182,192 Neoglycoenzymes application in lectin detection in solid-phase assays and glycohistochemistry (jack bean seeds, human carcinoma tissue), 182,447 Nervous system central and peripheral, major myelin proteins, separation by reverse-phase HPLC (rat), 180,59 Neurons motor, cell bodies, isolation from spinal cord stored at -70°C in ethylene glycol (frog, rat), 180,286 Neuropeptides cell cultures, bidimensional reverse-phase HPLC (bovine), 179,24 Neurophysin immunodetection on nitrocellulose, 183,250 Neutrophils ascorbic acid quantitation by HPLC with coulometric electrochemical detection (human), 181,276 Nickel ionic, interaction with peptides, evaluation by high-performance immobilized metal ion affinity chromatography, 183,159 ““Ni, binding to fatty acid, in assay for nanomole quantities of lipase,

179,413 Nick translation DNA immobilized on nylon Ninhydrin acid, in spectrophotometric

membranes, assay

178,260

of tryptophan

in intact

proteins,

177,16 reagent, acidic, in direct assay for bound sialic acids in sialoglycoproteins (mouse), 179,332; erratum, 182,459 Nitrates transport in plants, assay: WlO; for application as tracer, modified production method, 182,146 Nitroblue tetrazolium in assay of superoxide dismutase in tissue homogenates (rat mammary gland), 179,8 -5-bromo-4-chloro-3-indolylphosphate, as chromogenic substrate

INDEX

361

in hepatitis B virus DNA hybridization assay, comparison with luminescent substrate, 180,95 chloride salt, reduction mediated by NADH and phenazine methosulfate, role of superoxide anions, 176,170 Nitrocellulose antibodies eluted from blots, assay with gold reagents: application to electron microscopic immunocytochemistry, 183,225 antigen visualization by oxidative coupling reaction of N,N’-dimethyl-p-phenylendiamine and 4-chloro-l-naphthol, 183,s blotted mucus glycoproteins, calorimetric assay with periodateSchiff staining, 182,160 blotted proteins acylated, identification of covalently bound fatty acids, 183,220 colloidal gold staining, subsequent artifact appearance, 180,55 copper iodide staining, 181,250 detection by derivatization in situ with fluorescein isothiocyanate, 177,263 ‘H-labeled, detection by sodium salicylate-based Auorography,

178,327 quantitative immunoenzyme dot-blot assays, enhancement by SDS, 177,212 immobilized plasminogen activator, generation of plasmin, chromogenic assay with para-nitroaniline-derivatized peptide substrate, 177, 78 immunodetection of low-molecular-weight polypeptides, 183,250 unusual nucleic acids, 178,373 india ink staining of proteins and peptides, sensitivity improvement by fixation, 174,97; erratum, 178,215 quantitative nucleic acid hybridization on (Escherichia coli), 182, 280 tissue printing on, in histochemical localization of cysteine-rich proteins (barley), 182,334 visualization of acid phosphatase after electroblotting of polyacrylamide gels, 179,194 Noradrenaline, see Norepinephrine Norepinephrine deconjugation in plasma, suppression by ascorbic acid (human),

182,262 and related trace amines, assay by prechromatographic tion with naphthalene-2,3-dicarboxaldehyde

derivatiza(human urine),

180,279 Nuclear magnetic resonance 13C, and multiple ‘“C-labeling strategies, in detection of exogenous metabolites in presence of endogenous pools: glucose turnover measurements (human), 176,307 device for aeration and mixing of cell and organelle suspensions during, 182,151 500-MHz, in analysis of membrane lipids (sea urchin), 178,67 “P, in characterization of diastereoisomers of phosphate-ethylated dinucleoside monophosphates, 178,93 Nuclei large-scale isolation from oocytes (Xenopus laeuis), 180, 177 Nucleic acids hybridization assays employing dA-tailed capture probes: multiple capture methods (Wisteria monocytogenes), 181,345,360 in identification of microorganisms after labeling of test samples,

177,85 quantitative, on nitrocellulose filters (Escherichia coli), 182,280 reactions, glucose-6-phosphate dehydrogenase as label, 180,50 unusual, detection with monoclonal antibodies specific for triplexes, poly(ADP-ribose), DNA-RNA hybrids, and Z-DNA, 178,373 visualization on nylon membranes by uv shadowing, 178,41 Nucleoside monophosphates precipitation with lanthanum chloride, in assay for thymidine kinase, 178,38

362

CUMULATIVE

Nucleotides FAB/MS, low-nanogram detection, 178,102 phosphorothioate-modified, and plasmid DNA template, for oligonucleotide-directed mutagenesis technique, 179,309 plant, extraction, pre-HPLC purification, and HPLC analysis, procedural evaluation, 183,312 purine, precursors, chromatographic assay (murine Ll2lO cells),

182,121 Nylon blotting of biotin-labeled electrophoresed proteoglycans before and after enzymatic digestion (human aorta), 179,245 cationic, blots, in solid-phase assay for radiolabeled glycosaminoglycans in crude cell extracts (mouse), 179,361 electroblotting of low-density and very-low-density lipoproteins from composite gradient gels (human), 180,358 immobilized DNA, nick translation, 178,260 immobilized DNA-RNA hybrids, specific detection by modified primer extension method, 179,366 transferred low-molecular-weight growth factors, increase of retention by glutaraldehyde fixation, 178,255 visualization of nucleic acids by uv shadowing, 178,41 Zeta Probe, in immunodetection of unusual nucleic acids, 178, 373

SUBJECT

INDEX proteins,

preparation

and purification

(Trypanosoma

brucei),

181,l glucose-containing tetrasaccharide, affinity HPLC assay (human serum, urine), 182,48 glycosaminoglycan-derived, analysis by reverse-phase ion-pairing and ion-exchange HPLC with suppressed conductivity detection, 181,288 permethylated, with molecular mass to 2300, characterization by GC and GC-MS (porcine intestinal mucosa), 182,438 picomole amounts, HPLC with flow radioactivity detection, 178, 378 Olivomycins interaction with DNAs of different base composition and conformation, 179,401 Oocytes nuclei, large-scale isolation (Xenopus laeuis), 180,177 Organelle suspensions device for aeration and mixing during NMR studies, description,

182,151 Organophosphorus compounds low levels, microassay based

on acetylcholinesterase

inhibition,

180,380 Ornithine decarboxylase measurement by microassay

for

metabolically

generated

r4C02,

in glutathione

assay,

178,52 Orthophthalaldehyde -glutathione adduct, Octopamine dopamineand norepinephrine-related, assay by prechromatographic derivatization with naphthalene-2,3-dicarboxaldehyde (human urine), 180,279 Oligodeoxynucleotides directed cleavage and repair of single-strand vector: site-specific mutagenesis, 177,120 removal of Os-methylguanine and 04-methylthymine, measurement by immunoprecipitation, 183,74 sequence-specific synthetic, in gene assembly from partially overlapping fragments, 181,106 Oligomers galacturonic acid, from 3 to over 25 residues in length, ion-exchange chromatography, 178,248 5’-Oligonucleotide hydrazide derivatives, synthesis and use in preparation of enzyme-nucleic acid hybridization probes, 178,43 Oligonucleotides -alkaline phosphatase hybridization probes, preparation with 5’-oligonucleotide hydrazide derivatives, 178,43 dA-tailed capture probes, in nucleic acid hybridization assays: multiple capture methods (Wisteria monocytogenes), 181,345,360 directed mutagenesis, technique with plasmid DNA template and phosphorothioate-modified nucleotide, 179,309 ion-exchange chromatography with volatile buffer system, 178,320 synthetic as probe in assay of lysis and DNA hybridization in various bacteria, 181,23 as template primers in enzymatic assay for deoxyribonucleoside triphosphates, 180,222 Oligopeptides fluorescent, energy transfer assay, applications for neutral proteases, 183,50 Oligosaccharides asparagine-linked, from glycoproteins, determination of epitopes,

182,200 and

associated glycoproteins, characterization with endoglycosidases, review, 180,195 branched sialylated, from fetuin, high-pH anion-exchange chromatography with pulsed amperometric detection (bovine), 182,l components of glycosylphosphatidylinositol anchors of membrane

HPLC

with

Auorometry

179,236 as reagent in amino acid analysis, instability, 181,135 Osmotic pressure aqueous biopolymer solutions, osmometric analysis, 176,217 Ovalbumin hydrophobic interaction chromatography in alkaline pH, appropriate salts for, evaluation, 182,266 ‘I iodination, measurement by iodide microassay, 179, 138 Ovothiol A identification as efficient tyrosyl radical scavenger by qualitative fluorometric assay, 180,326 Oxazolones as substrates in active-site titrations of hydrolases, 181,321 Oxidation electrolytic, and aqueous column chromatography, purification and production of 36C10; for plant nitrate transport studies, 182, 146 Oxoglutarate spectrophotometric assay with algal NADPH-linked glutamate dehydrogenase, 180,99 Oxygen concentration in aqueous solutions, absolute value, enzymatic assay, 179,124

P

Paf-acether liquid chromatographic/electrochemical detection assay based on release of endogenous platelet serotonin (rabbit), 182,419 Palmitic acid tritium-labeled, TLC and sodium salicylate-based fluorographic detection, 178,327 Pancreas carboxypeptidase H, radiometric assay (rat), 178,27 Pantothenic acid in blood and milk, radiometric-microbiological assay (human),

178,63 Papain HPLC

assay in presence

of small

amounts

of product,

176,437

CUMULATIVE Paraoxon low levels,

microassay

based

on acetylcholinesterase

SUBJECT

micropreparative procedures for high-sensitivity sequencing, review, 177,221 in nanogram and subnanogram range, staining with AuroDye, FerriDye, and India ink, comparison, 182,44 para-nitroaniline-derivatized, in chromogenic assay of plasmin generated by plasminogen activator immobilized on nitrocellulose,

inhibition,

180,380 Paraoxonase, see Arylesterase Parathion active metabolite, hydrolysis by plasma paraoxonase/arylesterase, spectrophotometric assay (human), 180,242 Parathyroid hormone receptors characterization by preparation of photoreactive analog of bovine parathyroid hormone as ligand, 179,268 Parietal cells secretory physiology, microassay (rabbit), 182,346 Partitioning polymer, aqueous two-phase system, in isolation of plasma membranes from mammary gland (rat), 177,194 three-phase, in t-butanol/water/ammonium sulfate, in cathepsin D isolation (bovine spleen), 180,169 Parvalbumins in calibration of acidic isoelectric focusing gels (amphibian, fish),

178,121 Peak area integrated, and flow rate, product, in protein estimation (Escherichia coli), 182, 295 Penicillamine and related symmetrical and mixed disulfides, reverse-phase HPLC with electrochemical detection (human plasma, urine), 180, 259 Penicillin V hydroxylated by-products of fermentation, spectrophotometric assay: application to mutant penicillin producer strains on agar,

179,288 Pentoses estimation by phenol-sulfuric Peptide mapping collagen chains: cyanogen

acid reaction, bromide

cleavage

reevaluation, in polyacrylamide

181,18 gels,

176,365 complement component C3 after electroblotting onto polyvinylidene difluoride membranes from Tricine-SDS-PAGE, 181,33 by cyanogen bromide fragmentation, application of SDS-polyacrylamide minigel system, 178,391 proteolytic digests by ion-exchange chromatography on sulfoethyl aspartamide column, 176,255 Peptides antigenic, simultaneous purification-conjugation, synthesis strat-

egy,181,389 N-bromoacetyl-modified, automated synthesis, in preparation of synthetic peptide polymers, peptide-protein conjugates, and cyclic peptides, 177,373 carboxyl-terminal, isolation from proteins by diagonal electrophoresis, application to bacterial entomocidal toxin, 181,212 competitive ELISA, analysis, 183,283 degraded with carboxypeptidases MI1 and Y and analyzed by ‘%f plasma desorption mass spectrometry, C-terminal sequence determination, 180,28 electrophoretic mobility in free-solution capillary electrophoresis, semiempirical model, 179,28 generation from proteins separated by SDS-PAGE, 178,233 hydrolysis in hermetically sealed microcapillary tube: high recovery of labile amino acids (bovine, chicken), 182,383 hydrophilic, derivatization for liquid secondary-ion mass spectrometry at picomole level, 182, 165 immobilized synthetic, in affinity purification of protein-serine kinases (rabbit muscle), 180,237 india ink staining on nitrocellulose, sensitivity improvement by fixation, 174,97; erratum, 178,215 interaction with Cu(II), Ni(II), and Zn(II), evaluation by high-performance immobilized metal ion affinity chromatography, 183, 159

363

INDEX

177,78 synthesis, by-products, identification by MS, 180,387 synthetic dansylated inhibitor, fluorescence energy transfer of human renin to, in application of assay for enzyme-competitive inhibitor dissociation constants from displacement curves,

181,172 Percoll gradients isolation of distinct renal cortical toxicity studies (rat), 182,271 Periodate and iodate, simultaneous assay application to carbohydrate Peroxidase labeled collagen, in spectroscopic stained blots, sensitivity, lOO-fold der ultraviolet light, 182,176 substrates, role in speed increase

cell populations

for chemical

by capillary zone analysis, 177,62 collagenase enhancement

electrophoresis,

assay, 176,150 of visualization

of flow-injection

cyto-

binding

unassays,

181,379 PH alkaline, in hydrophobic interaction chromatography, evaluation of salts for, 182,266 Ampholine gradient, uroporphyrinogen-I synthase in, preparative free solution isoelectric focusing (human erythrocytes), 182,

217 computation in complex biochemical and gas-liquid systems, algorithms, 181,220 matrix, estimation in mitochondria with fluorescent probe (bovine heart), 178,348 of resolving gel, effect on PAGE of high-molecular-weight myofibrillar proteins (bovine, rabbit), 180, 205 PhastSystem in agarose isoelectric focusing of plasma low- and very-low-density lipoproteins (human), 182,94 Phenazine methosulfate, see N-Methylphenazonium methosulfate Phenol -chloroform mixtures in extraction of DNA in silica gel polymer-containing tubes, 183, 13 high-temperature, in preparation of RNA from unspheroplasted yeast cells, 176,303 -sulfuric acid reaction for estimation of hexoses and pentoses, reevaluation, 181, 18 Phenylalanine plasma, calorimetric assay with L-phenylalanine dehydrogenase: application to monitoring phenylketonuria, 180,91 L-Phenylalanine dehydrogenase in calorimetric assay for plasma phenylalanine: application to monitoring phenylketonuria, 180,91 in enzyme cycling assay for phenylpyruvate in tissue extracts (rat),

183,210 Phenylalanine hydroxylase mRNA base changes, detection and localization by chemical cleavage method (rat), 183,263 Phenylboronic acid immobilized on 3-isothiocyanatopropyltriethoxysilane-activated nonporous monodisperse silicas, affinity chromatography, 179, 171 Phenyl isothiocyanate standard reagent, and subpicomole HPLC, in automated polypeptide sequencing, 183,290

364

CUMULATIVE

Phenylketonuria monitoring, application of calorimetric assay for plasma phenylalanine with L-phenylalanine dehydrogenase (human), 180,91 Phenylpyruvate enzymatic cycling assay in deproteinized tissue extracts (rat brain, kidney, liver), 183,210 Phosphate -alumina complexes for immobilization of biomolecules, review,

180,l excess, pyrophosphate calorimetric assay in presence of, application to mitochondrial pyrophosphate metabolism, 176,390 inorganic assay by flow injection with immobilized enzymes and chemiluminescence detection, 182,366 selective extraction, concentration, and calorimetric assay from microliter quantities of cultured mammalian cells, 181,130 Phosphatidate picomole levels, enzymatic assay (rat hepatocytes), 180,24 Phosphatidylcholine HPLC and GC-MS assay (human, rat), 180,85 Phosphatidylinositol4,5-bisphosphate derivatization with 9-anthryldiazomethane, reverse-phase HPLC (rat brain), 179,127 Phosphatidylinositol kinase interaction with nonionic polyoxyethylene detergents, 179,66 Phosphatidylinositol4-phosphate derivatization with 9-anthryldiazomethane, reverse-phase HPLC (rat brain), 179,127 Phosphatidylserine decarboxylase DEAE-cellulose chromatographic assay (yeast), 182,452 Phosphoamino acids analysis in gel-fractionated phosphoproteins, facilitation by acid/ base hydrolysis on Immobilon, 176,22 resolution in protein hydrolysate by TLC, 177,138 Phosphocholine HPLC and GC-MS assay (human, rat), 180,85 Phospholipase A, continuous fluorometric assay with pyrene-labeled phospholipids in presence of serum albumin, 177,103 plasmalogen-hydrolyzing, continuous spectrophotometric assay with coupling enzymes (snake venom), 176,180 Phospholipids assay with Victoria blue R and B (Micrococc~~ lysodeiktieus), 180, 186 classes, and arachidonic acid, HPLC separation on cyanopropyl columns (bovine, chicken, rat, soybean), 182,32 interference in Lowry assay of proteins, elimination procedure,

183,275 pyrene-labeled, in continuous fluorometric assay for phospholipase A2 in presence of serum albumin, 177,103 Phosphomannosyl receptors testicular P-galactosidase fractions with high affinity for, purification (bovine), 182,432 Phosphoproteins gel-fractionated, phosphoamino acid analysis, facilitation by acid/ base hydrolysis on Immobilon, 176,22 Phosphorothioate modified nucleotide, andplasmidDNA template, in oligonucleotidedirected mutagenesis technique, 179,309 Phosphorylation fusion protein IFN-aA/y (human), 178,342 proteins, evaluation by combined vertical slab gel focusing and immunoblotting, 183,144 Pbotinus-luciferin 4-monooxygenase (ATP-hydrolyzing) application to interleukin-2 gene promoter, reporter gene, 176,28 Photobiotin labeled restriction fragments, in cross-hybridization method for DNA mapping, 178,273

SUBJECT

INDEX

Photobleaching laser, in production of fluorescence-grade adenosine deaminase (bovine intestinal mucosa), 18 1,383 Photographic method in detection of DNA fragments produced by restriction enzymes,

177,199 Photometry in continuous assay of cellulase with 4-methylumbelliferyl-~-D-cellobioside as substrate (Trichoderma reesei), 179,186 o-Phthalaldehyde derivatized amino acids formation and instability, review, 178,l high-sensitivity analysis with fluorescence detection: application to protein structure determination, 178,227 derived isoindoles, formation and degradation, kinetic-spectrophotometric study, 180,172 o-Phthaldialdehyde derivatized ACV synthetase, HPLC (Streptomyces chuligerus),

178,399 Phycoerythrin fluorescence, in assay for peroxy radicals: screen for biologically evant protective agents, 177,300 Physostigmine in plasma, HPLC with fluorescence detection (human), 207 Pituitary carboxypeptidase H, radiometric assay (rat), 178,27 Plants barley, seed tissues, printing on nitrocellulose, in histochemial calization of cysteine-rich proteins, 182,334 cabbage, glucosyltransferase and sulfotransferase, radioassay,

rel-

177,

lo-

178,

137 jack bean, solid-phase assay with neoglycoenzyme, 182,447 nucleotides, extraction, pre-HPLC purification, and HPLC analysis, procedural evaluation, 183,312 radish, viable cell density, chemiluminescent assay, 181,149 various, bound selenomethionine, trace levels, indirect determination, 178,77 Plasma, see also Blood, Serum apolipoproteins A-I and A-II, purification from precipitates obtained by cold ethanol fractionation (human), 178,301 choline and metabolite levels, HPLC and GC-MS assay (human, rat), 180,85 deconjugation of noradrenaline and dopamine, effects of ascorbic acid (human), 182,262 glucose oxidase, direct electrochemical quantitation (mouse), 178, 427 glutathione SH groups, assay by electron spin resonance (mouse, rat), 182,58 histamine and W-methylhistamine, GC-MS (dog, human), 178, 414 homocysteine HPLC with fluorescence detection (human), 178,208 and penicillamine and related symmetrical and mixed disulfides, reverse-phase HPLC with electrochemical detection (human),

180,259 lipoproteins, low- and very-low-density, agarose isoelectric focusing with PhastSystem (human), 182,94 metabolic intermediates of lactose synthesis, bioluminescent microanalysis (human), 176,449 methotrexate and trimetrexate levels in cancer patients, flow cytometric bioassay with fluorescein-conjugated methotrexate,

178,287 paraoxonase/arylesterase hydrolysis of active pyrifos and parathion, spectrophotometric

metabolites assays

of chlor(human),

180,242 phenylalanine, calorimetric assay with L-phenylalanine nase: application to monitoring phenylketonuria, phenylpyruvate, enzymatic cycling assay (rat), 183,210

dehydroge-

180,91

CUMULATIVE physostigmine,

HPLC

with

fluorescence

detection

(human),

SUBJECT 177,

207 protein-associated

sulfur,

GC with

flame

photometry

(human,

rat),

178,394 pyridoxal5’-phosphate, enzymatic-HPLC assay (human), 181,267 urate oxidase, radiochemical assay with HPLC (human), 176,290 Plasmalogens choline, hydrolyzing phospholipase AZ, continuous spectrophotometric assay with coupling enzymes, 176,180 Plasmids chimeric, with ampicillin resistance gene, in yeast cells, p-lactamase iodometric detection, 177, 165 expressed proteins lacking functional assay systems, purification (Escherichiacoli), 181,336 insert DNA sequences, large-scale purification by low-percentage agarose exclusion chromatography, 179,167 supercoiled DNA, bidirectional sequencing, 183,258 Plasmin, see Fibrinolysin Plasminogen activator immobilized on nitrocellulose, generation of plasmin, chromogenic assay with paru-nitroaniline-derivatized peptide substrate,

177,78 tissue-type and urokinase-type, in serum-free cell culture-conditioned media, simultaneous calorimetric assay, 177,341 urokinase-type, HPLC assay in presence of small amounts of product, 176,437 Plasminogen activator inhibitors and plasminogen activators, simultaneous calorimetric assay in serum-free conditioned culture media, 177,341 Platelet-activating factor HPLC/fluorescence detection assay, 180,131 Platelets 32P-labeled, inositol phosphate production, analysis by ion-pair reverse-phase HPLC, 179,90 serotonin release, in paf-acether assay by liquid chromatography with electrochemical detection (rabbit), 182,419 Polyacrylamide gels Coomassie staining one-step low-background procedure, 182,157 proteins Western-blotted from, luminescent immunodetection,

179,206 detection

of fungal

cellulase

with

5-bromoindoxyl-P-D-cellobioside,

182,250 direct detection dried, preparation enzyme catalytic

of &1,3-glucanase isozymes (tobacco), 182,136 for densitometry and/or storage, 179,242 function in, nondestructive assay by product

blot,

178,22 fluorescent labeling of polypeptides for laser excitation, 179,291 immobiline-containing, in preparative isoelectric focusing of leukoregulin, comparison with ampholine columns, 177,358 miniaturization, improvement of protein visualization and autoradiographic detection, 177,287 multiwell substrate-immobilized, in assay of solid-phase tyrosinespecific protein kinase, 182,37 peptide mapping of collagen chains: cyanogen bromide cleavage method, 176,365 protein detection, quantitation, and preparative elution by ultraviolet scanning densitometry, 176,400 proteins fractionated on, electroblotting on nitrocellulose, in visualization of acid phosphatase, 179,194 selective silver staining of urease (Canaualia erhsiformis), 177,37 sodium dodecyl sulfate-, see Sodium dodecyl sulfate-polyacrylamide gels staining of pyridine nucleotide-linked dehydrogenases, 178,243 Poly(ADP-ribose) monoclonal antibodies, in detection of unusual nucleic acids, 178,

373

365

INDEX

Polyamines fluorometric assay with electrophoresis on Titan III cellulose acetate (rat tissues, urine), 180,307 Polycarbonate membranes in fractionation and analysis of mitochondria (rat liver), 179,114 Polyethylene glycol miniaturization of polyacrylamide gels for improvement of protein visualization and autoradiographic detection, 177,287 Polyhydrazides polyacrylic, as reagents for glycoprotein detection, 181,182 Polymerase chain reaction automated, construction of cyclotherm apparatus for, 181,163 generation of radiolabeled DNA and RNA probes, 177,90 modification, in general method of site-specific mutagenesis (Ther-

musaquaticus),180,147 Polymers peptide, synthetic, preparation with N-bromoacetyl-modified peptides, 177,373 superabsorbent, in enzymatic assays for ethyl alcohol in urine (human), 182,257 supported amino groups, spectrophotometric estimation, 180,253; erratum, 183,197 Polynucleotides solution-phase detection with interacting fluorescent labels and competitive hybridization, 183, 231 Polyoxyethylenes as nonionic detergents in studies of phosphatidylinositol kinase, analysis, 179,66 Polypeptides automated sequencing with standard phenyl isothiocyanate reagent and subpicomole HPLC, evaluation, 183,290 containing disulfide bonds, cyanogen bromide cleavage at methionine residues, 177,161 electrospray ionization-MS and capillary electrophoresis-MS,

179,404 fluorescent-labeled, 291 low-molecular-weight,

laser

excitation

in polyacrylamide

immunodetection

gels,

on nitrocellulose,

179, 183,

250 picomole quantities, simultaneous manual sequencing, application of HPLC analysis of 4-N,N-dimethylaminoazobenzene 4’-thiohydantoin amino acids at subpicomole levels, 177,46 Polysaccharides complex bacterial, sugar residues, absolute configuration, determination by circular dichroism spectroscopy, 179,209 Polyvinylidene difluoride membranes in assay of cellular transglutaminase (human, rat), 182,170 immobilized proteins, phosphoamino acid analysis, 177,407 myoglobin electroblotted on, gas-phase sequencing for assignment of correct molecular weights to markers (equine heart, sperm whale), 183, 1 peptide electroblotting from Tricine-SDS-PAGE: application to human complement component C3,181,33 proteins electroblotted from SDS-polyacrylamide gel, amino acid analysis, 179,50, 181, 75 Porphyrin carboxylic acids, and zinc protoporphyrin, simultaneous assay in urine by reverse-phase HPLC with fluorometry (human), 183,

134 Postcolumn reaction with HPLC, thiobarbituric acid-reactive substances in oxidized lipids (rat liver), 182,116 Potassium thiocyanate -cesium thiocyanate high-resolution equilibrium gradients, in separation of density-labeled and unlabeled proteins, 177,

333 Precipitation nucleoside monophosphates with thymidine kinase, 178,38

lanthanum

chloride,

in assay

for

366

CUMULATIVE

Primer extension modified procedure for specific detection of DNA-RNA hybrids on nylon membranes, 179,366 L-Proline addition to quinones, in chromogenic assay for catechol oxidases,

179,375 Pro-opiomelanocortin pituitary, extraction and purification, application of trace-enrichment technique for loading gel-permeation HPLC columns (bovine, human pituitary), 179,222 Protease digested proteins, peptide mapping by strong-cation-exchange chromatography on sulfoethyl aspartamide column (Staphylococcus aureus V8), 176,255 fluorescent oligopeptide energy transfer assay (Astacus fluuiatilis),

183,50 Proteinase inhibitors detection in fractions after liquid chromatography, 179,349 Protein determination bicinchoninic acid method in astrocyte monolayer cultures (rat brain), 177,41 elimination of interfering substances, 180,136 Bradford method, sensitivity and variability, effects of detergents,

178,263 Coomassie blue method nonlinearity assessment, 179,198 refinement, 177,100 by fourth-derivative absorption spectroscopy during purification (bovine, Neisseria meningitidis), 182,141 Lowry method, elimination of interferences, 183,275 Protein-glutamine y-glutamyltransferase cellular, calorimetric assay (human, rat), 182,170 Protein kinase calmodulin-dependent type II, detection in SDS-polyacrylamide gel, 183,139 tyrosine-specific, solid-phase assay in multiwell substrate-immobilized polyacrylamide gel, 182,37 Proteins actin-binding, two-dimensional gel electrophoresis with SDS, 178, 32 acylated, immobilized on nitrocellulose, identification of covalently bound fatty acids, 183,220 affinity chromatography on 3-isothiocyanatopropyltriethoxysilaneactivated nonporous monodisperse silicas with immobilized ligands, 179,171 aqueous solutions, osmometric analysis, 176,217 associated sulfur, GC with flame photometry (human, rat), 178,394 binding of ligands, assay by small volume equilibrium dialysis system, 176,157 blotted on nitrocellulose, copper iodide staining, 181,250 blotting procedures, y-globulin-derived standards for determination of molecular weights, transfer, and immunodetection efficiencies, 176,249 calmodulin-binding, detection with metabolically %-labeled recombinant calmodulin, 178,141 carboxyl-terminal peptides, isolation by diagonal electrophoresis, application to bacterial entomocidal toxin, 181,212 chromatographic separations on immobilized metal ion stationary phases (human serum), 183, 108 reversible conversion of octadecyl-bonded silica to ion-exchange surfaces, 176,191 clathrin assembly, AP180, identification in crude brain extracts by two-dimensional SDS-PAGE (bovine), 179,352 conjugates, molecular ratio, determination by amino acid analysis and least-squares computer method, 179,158 cyst(e)ine-containing, reduction and fluorescent labeling prior to structural analyses, 180,231

SUBJECT

INDEX

cysteine and half-cystine content, determination after hydrochloric acid hydrolysis with disulfide compound as additive, 177,318 cysteine-rich, histochemical localization by tissue printing on nitrocellulose (barley), 182,334 density-labeled and unlabeled, separation by high-resolution KSCN/CsSCN equilibrium gradients, 177,333 deuterium-labeled tryptophan content, assay by HPLC-thermospray MS (Halobacterium halobium), 181,271 digestion by Staphylococcus aureus V8 protease, peptide mapping by strong-cation-exchange chromatography on sulfoethyl aspartamide column, 176,255 DNA binding, and specific genomic DNA target sites, simultaneous characterization by South Western blot mapping (mouse), 179,

299 electroblotted onto polyvinylidene polyacrylamide gel, amino electrospray ionization-MS

difluoride acid analysis, and capillary

membranes

from

179,50;

181,75

SDS-

electrophoresis-MS,

179,404 extinction

coefficients,

calculation

from

amino

acid sequence

data,

182,319 extraction from green plant tissues for two-dimensional resis, 177, 33 fusion, IFN-oA/r, construction and phosphorylation

electropho(human),

178,342 in gingival crevicular fluid, electrophoresis: data refinement by clustering (human), 179,106 guanine nucleotide-binding G., competitive ELISA, 176,185 hydrolysate, phosphotyrosine, phosphoserine, and phosphothreonine resolution by TLC, 177,138 hydrolysis in hermetically sealed microcapillary tube: high recovery of labile amino acids (bovine, chicken), 182,383 immobilized on nitrocellulose, detection by derivatization in situ with fluorescein isothiocyanate, 177,263 oxidized crosslinked Sepharose preparations by reductive amination, 180,114 polyvinylidene difluoride membranes, phosphoamino acid analysis, 177, 407 india ink staining on nitrocellulose, sensitivity improvement by fixation, 174,97; erratum, 178,215 intact, tryptophan spectrophotometric assay by acid ninhydrin method, 177,16 iodination, measurement by iodide microassay, 179,138 isoelectric points, theoretical determination, 179,319 isolation and sequence analysis with 2-D gel electrophoresis coupled to high-pressure liquid extrusion (murine forebrain), 180,340 major, central and peripheral nervous system myelin, reverse-phase HPLC (rat), 180,59 micropreparative procedures for high-sensitivity sequencing, review, 177,221 mixtures, quantitative analysis by second-derivative absorption spectroscopy (bovine lens), 18 1,79 modification by diazotized arsanilic acid: identification of modified amino acids, 177,183 myofibrillar, high-molecular-weight, factors affecting PAGE and electroblotting (bovine, rabbit), 180,205 in nanogram and subnanogram range, staining with AuroDye, FerriDye, and India ink, comparison, 182,44 nuclear sequential two-dimensional and acetic acid/urea/Triton X-100 gel electrophoresis (Caenorhubditis elegant), 177,388 and thymic, microgel immunoblotting by unidirectional diffusion,

176,350 oxidatively modified, carbonyl groups, determination by reduction with tritiated sodium borohydride, 177,419 oxidized, y-glutamyl semialdehyde residues in, derivatization by fluoresceinamine, 182,226 oxygen-sensitive, low-temperature electron paramagnetic reso-

CUMULATIVE nance spectroelectrochemical cell titration, apparatus description, 18 1,283 -peptide conjugates, preparation with N-bromoacetyl-modified peptides, 177,373 phosphorylation state, evaluation by combined vertical slab gel focusing and immunoblotting, 183,144 plasma membrane, identification by cell surface labeling and 2-D PAGE (Dictyostelium discoideum), 179,37 plasmid-expressed, lacking functional assay systems, purification (Escherichia coli), 181,336 -protein stable crosslinks, generation by fluorescein-photosensitized reaction (human), 179,154 p21”, enzyme-linked immunoblot assay: technique for dissection of gene family expression, 180,158 quantitative immunoenzyme dot-blot assays on nitrocellulose, enhancement by SDS, 177,212 ribosomal, estimation by product of integrated peak area and flow rate (Escherichia coli), 182,295 salt-promoted thiophilic adsorption on sulfur-containing agarose, implications for covalent chromatography, 176,330 S, cDNA, site-specific mutagenesis by modification of Thermus aquaticus polymerase chain reaction (human), 180,147 SDS-PAGE, subsequent peptide production, 178,233 as self-quenched fluorogenic substrates, in zymography of cathepsin D, trypsin, and chymotrypsin, preparation, 176,261 solutions, alkyl sulfates in, analysis by isocratic and gradient ion chromatography, 182,103 stained by metal salts on SDS-polyacrylamide gels, Western blotting, 180,311 structure determination, application of amino acid analysis by derivatization with o-phthalaldehyde and 9-fluorenylmethyl chloroformate and fluorescence detection, 178,227 thiols, irreversible reaction with l-p-chlorophenyl-4,4-dimethyl-5diethylamino-1.penten-3.one hydrobromide, characterization,

177,237 tritium-labeled, nitrocellulose-blotted, sodium salicylate-based fluorographic detection, 178,327 two-dimensional polyacrylamide gel electrophoresis in presence of benzyldimethyl-n-hexadecylammonium chloride and sodium dodecyl sulfate, 176,457; erratum, 180,194 unfolded, sequential SDS-PAGE and reverse-phase HPLC, 178, 385 in unstained gels, detection, quantitation, and preparative elution by ultraviolet scanning densitometry, 176,400 visualization and autoradiographic detection, improvement bypolyacrylamide gel miniaturization, 177,287 Western-blotted from Coomassie blue-stained polyacrylamide gels, luminescent immunodetection, 179,206 Western immunoblotting, temperature-dependent reduction in background staining (human milk), 177,256 Protein sequencing amino-terminal, after electroblotting onto polyvinylidene difluoride membranes from Tricine-SDS-PAGE: application to human complement component C3,181,33 application of 4,4-N,N-dimethylaminoazobenzene-4’-isothiocyanate micromethod, improvements, 180,374 2-D SDS-PAGE with high-pressure liquid extrusion (murine forebrain), 180,340 automated, with standard phenyl isothiocyanate reagent and subpicomole HPLC, evaluation, 183,290 C-terminal, peptides degraded with carboxypeptidases Y and MB and analyzed by plasma desorption mass spectrometry, 180,28 derived data, in calculation of protein extinction coefficients, 182, 319 high-sensitivity, micropreparative procedures, review, 177,221 at picomole level, application of reverse-phase HPLC of dimethyl-

SUBJECT

INDEX

367

aminobenzene sulfonyl and thiocarbamoyl derivatives of amino acids, 178,107 thioacetylation method: derivatization of 2-methyll5(4H)-thiazolones for HPLC, 181,113 Protein synthesis de nouo rates, and steady-state levels, simultaneous measurements by immunoblotting (chicken embryo hepatocytes), 179,284 Protein-tyrosine kinase nonradioactive dot-blot assay (human breast), 182,98 Proteoglycans biotin-labeled, electrophoretic separation on minigels and blotting onto nylon membranes before and after enzymatic digestion (human aorta), 179,245 synovial fluid, profiles obtained by electrophoresis and triple immunoblotting (human), 180,211 Proteolysis casein, measurement by spectrofluorometry (bovine milk), 176,239 Prothymosin a gene intron mapping by exon excision (human), 183,64 Protozoa E&amoeba histolytica, pinocytic vesicles of defined age, magnetic separation, 182,77 Trypanosoma cruzi, exponentially growing population, total DNA per cell, quantitation by diphenylamine reaction and flow cytometry, 177,353 Purine-nucleoside phosphorylase immobilized, and chemiluminescence detection, in flow injection assay of inorganic phosphate, 182,366 isotopic assay based on [2-3H]inosine as substrate and release of tritium, 183,279 Purines precursors in L1210 leukemia cells, ion-exchange chromatography (mouse), 182,121 Putrescine fluorometric assay with electrophoresis on Titan III cellulose acetate (rat tissues, urine), 180,307 Pyrene labeled fatty acids, acylated lipids with, HPLC, 178,166 labeled phospholipids, in continuous fluorometric assay for phospholipase A, in presence of serum albumin, 177,103 Pyridine dinucleotides in hepatocyte cultures, reverse-phase HPLC (rat), 179,34 Pyridine nucleotides fluorescent derivatives, formation during glutamate dehydrogenasebased assay for L-glutamic acid, 180,248 Pyridoxal5’-phosphate effect on soluble and membrane-bound glutamate decarboxylase binding to liposomes, radiochemical microassay (rat brain),

182,64 enzymatic-HPLC assay (human plasma), 181,267 N-2-Pyridylglycamines borate complexes, reducing monosaccharides as, capillary zone electrophoresis, 176, 72 Pyrimidines dimers, in DNA, assay by HPLC/GC with electron capture detection, 182,424 Pyrophosphate calorimetric assay in presence of orthophosphate excess, application to mitochondrial pyrophosphate metabolism, 176,390 Pyruvate carboxylase identification by transblot method (rat liver), 177,s~ Pyruvate dehydrogenase and cytochrome-c oxidase, deficiencies, distinction by determination of [U-%]glucose metabolism (human fibroblasts), 176, 382

368

CUMULATIVE

Pyruvate kinase coupled assay, for study 396 HPLC assay in presence

of mammalian of small

hexokinase

amounts

regulation,

of product,

SUBJECT

177,

176,437

Q

INDEX

Renaturation 20/3-hydroxysteroid dehydrogenase denatured by urea, size-exclusion chromatographic analysis, 180,181 Renin fluorescence energy transfer to synthetic dansylated inhibitor peptide, in application of assay for enzyme-competitive inhibitor dissociation constants from displacement curves (human),

181,172 Quinoline N-substituted terization

chloride-sensitive as fluorescent

compounds, synthesis indicators for biological

and characapplications,

178,355 Quinone addition

of L-proline,

in chromogenic

assay

for catechol

oxidases,

179,375 R Radioassay bile acid CoA:glycine/taurine N-acyltransferase with n-butanol solvent extraction (human liver), 182,360 glucosyltransferase and sulfotransferase involved in glucosinolate biosynthesis (cabbage), 178,137 trypsin and trypsin-like activities, 179,56 Radiochemical assay with HPLC, urate oxidase (human plasma), 176,290 Radioimmunoassay 1,25-dihydroxyvitamin Ds receptor, 183,57 dUTP in thymidylate synthase-inhibited cell extracts (human lung carcinoma cells), 177,347 Radioiodination with N-iodoacetyltyramine by alkylation of sulfhydryl groups, procedures, 179,389 selective, endothelial cell apical and luminal surfaces with iodogencoated Sephadex G-25, in oitro and in situ studies (bovine, rabbit aorta), 179,274 Radioisotopic assay plasmin and DNase with labeled human fibroblasts on microcarriers, 182, 20 Radiolabeling in analysis of inositol phosphates, review, 183,199 DNA with [3H]thymidine, in quantitation of anchorage-independent growth in suspension culture system (rat fibroblasts), 177,95 and RNA with s*P: generation of labeled probes by polymerase chain reaction, 177,90 Radiometric assay carboxypeptidase H and other carboxypeptidase B-like enzymes (rat brain, pancreas, pituitary), 178,27 glycerol, glucose, and glycogen, 180,ll with microbiological assay, pantothenic acid in blood and milk (human), 178,63 Raman spectroscopy surface-enhanced, in immunoassay for binding between biomolecules with mutual affinity, 182,386 Reconstitution mitochondrial tricarboxylate carrier, subsequent spectroscopic enzymatic assay (bovine liver), 177,323 Recycling repetitive, GTP cyclohydrolase I for synthesis of dihydroneopterin triphosphate, 176,15 Reduction cyst(e)ine-containing proteins with tributyl phosphine before struttural analyses, 180,231 in isotopic labeling of antibiotics, 181,90 oxidatively modified proteins with tritiated sodium borohydride, in determination of carbonyl groups, 177,419

Restriction endonucleases codigested DNA fragments, 413 Restriction mapping DNA, cross-hybridization

discriminatory

method

with

32P 3’-end labeling, photobiotin-labeled

177, probes,

178,273 Reverse-phase chromatography amino acids after derivatization with 1-fluoro-2,4-dinitrophenyl-5L-alanine amide, 178,17 a-amino acids after boroxazolidone formation, 178,276 ammonia-assimilating enzymes from cyanobacteria in vitro situ, 180,152 benzoylated inositol, in assay of L-myo-inositol-l-phosphate thetase, 178,135 bidimensional, atria1 natriuretic factor (bovine chromaffin

and in syncells),

179,24 bilirubin conjugates (canine bile), 183,269 cholesterol 7a-hydroxylase, simultaneous assay with endogenous and exogenous [4-“‘C]cholesterol as substrate (rat liver), 182, 212 cytosolic glutathione S-transferase and microsomal epoxide hydrolase activities toward benzo[a]pyrene-4,5-oxide, 178,153 diaminopimelate epimerase and decarboxylase simultaneous assay (Bacillussubtilis), 180, 298 diastereoisomers of phosphate-ethylated dinucleoside monophosphates, 178,93 dimethylaminobenzene sulfonyl and thiocarbamoyl amino acid derivatives for amino acid analysis and microsequencing at picomole level, 178,107 direct-injection, microassay for kinetics of terminal reactions of tetracycline biosynthesis (Streptomyces aureofaciens), 181,125 with electrochemical detection, homocysteine and penicillamine and related symmetrical and mixed disulfides (human plasma, urine), 180,259 with Auorometry in collagen nonisotopic evaluation in fibroblast cultures (human),

176,395 hepatic folates (rat), 176,406 zinc protoporphyrin and porphyrin carboxylic acids in urine, simultaneous asay (human), 183,134 ion-pair and affinity chromatography, in analysis of tissue folate composition (bovine, human, rat), 182,84 with fluorometry, aliphatic amino acid decarboxylases, 179,182 and ion-exchange HPLC with suppressed conductivity detection, in assay of glycosaminoglycan-derived oligosaccharides, 181, 288 isocratic, in assay of purified aryl sulfotransferase, suitability for reactions yielding unstable sulfuric acid esters, 183,320 [32P]inositol phosphates: optimization and biological applications, 179,90 with radioactivity detection, dihydroorotase, 176,105 in isolation of modified histone H3 from uv-irradiated deoxyribonucleoprotein, 178,88 major proteins of nervous system myelin (rat), 180,59 methylated histidine in actin (chicken, mammals), 178,194 narrow-bore, with 2-D SDS-PAGE, in protein isolation and sequence analysis (murine forebrain), 180,340 and normal HPLC, tocopherol and tocotrienol isomers, 180, 368

CUMULATIVE

SUBJECT

polyphosphoinositides derivatized with 9-anthryldiazomethane (rat brain), 179,127 with pulsed amperometric detection and membrane reactor, assay of cyclodextrins and branched forms, 179,336 pyridine dinucleotides in hepatocyte cell cultures (rat), 179,34 with radioactive flow detector, in identification of fatty acids covalently bound to acylated proteins, 183,220 unfolded proteins after SDS-PAGE, 178,385 with uv detection, desferrioxamine-available iron in tissues (rabbit), 180,126 Rhodamine B labeled thyroid hormone, formation of high-molecular-weight aggregates in solution: possible source of artifacts in binding experiments, 176,440 Ribonuclease A active site, binding to &dine 2’-monophosphate, analysis with titration calorimeter, 179,131 carboxyl-terminal peptides, isolation by diagonal electrophoresis,

181,212 hydrophobic interaction chromatography ate salts for, evaluation. 182.266 Ribonuclease inhibitor purification, characterization, and direct (porcine brain), 176,175 Ribosomes complex C, recovery from cellulose nitrate

in alkaline

pH,

spectrophotometric

membranes

appropri-

assay

(Escherichia

coli),176,278 protein, estimation by product of integrated peak area and flow rate (Escherichia coli), 182,295 RNA assays, application of guanine determination by HPLC with electrochemical detection (rat), 180,38 base changes, detection and localization by chemical cleavage method (human, mouse, rat), 183,263 biotinylated biotin molecule number and distribution, estimation by gel retardation assay, 179,98 and fluoresceinated, as probes for detection of DNA targets: effects of derivatization extent on assay sensitivity, 182,309 probes in DNA immunodetection: monoclonal antibody reactivity to DNA-RNA hybrids, 181,96 in cell lysates, assay by noise-free molecular hybridization (human mononuclear cells), 181,371 -DNA hybrids enzyme immunoassay for nonisotopic detection of RNA, 181, 153 monoclonal antibodies, in detection of unusual nucleic acids,

178,373 specific detection on nylon membranes by modified primer extension method, 179,366 and DNA, simultaneous purification from small numbers of tissue culture cells (human, mouse), 180,303 eukaryote, assay by colony blot technique, 176,78 messenger, and antigenic epitopes, simultaneous localization: extracellular matrix gene expression by cultured cells (human), 178, 184 preparation from unspheroplasted yeast cells by homogenization in high-temperature phenol:chloroform mixtures (Saccharomyces cereuisiae), 176,303 quantitative hybridization on nitrocellulose filters (Escherichia coli), 182,280 radiolabeled probes, generation by polymerase chain reaction, 177, 90 ribosomal, in situ hybridization to metaphase chromosomes, optimization (human), 182,25 total cellular, from skeletal tissues, isolation and analysis (rat),

183,301

INDEX

369

transfer, interference with DNA-4’,6-diamidino-2-phenylindole complex, fluorescence assay, 176,416 unfractionated, microgram quantities, rapid amplification of circular DNA from, 177,7 Ruthenium(B) -bathophenanthroline complexes, use as nonradioactive labels in dideoxy DNA sequencing, 181,216 Ryanodine binding to skeletal muscle membranes: characterization of ion channel activity (rabbit), 183,31

s Saliva cotinine, idiotype-anti-idiotype immunoassay (human), 182,187 Sanfilippo D syndrome diagnosis by estimation of acetylglucosamine-6-sulfatase with 0-(a-N-acetylglucosamine+sulfate)-(1 --f 3)-L-[6-3H]idonic acid (human), 176,244 Sedimentation equilibrium thermal convection in, stabilization by metrizamide, 178,198 Seeds whole, free amino acid assay (peanut), 180,264 Selenate as substrate in radioactive electrophoretic assay of ATP-sulfurylase in crude extracts, 176,294 Selenomethionine bound, trace levels, indirect determination in plants and biological materials, 178,77 metabolites, synthesis and HPLC analysis, 179,396 Semimicrotitration cholinesterase assay device (rat liver, plasma), 179,120 Sephadex G-25 iodogen-coated, in selective radioiodination of endothelial cell apical and luminal surfaces in oitro and in situ (bovine, rabbit aorta), 179,274 Sepharose bonded cleavable assayable linking molecules, chemistry: definition of chemical events and ligand titers, 181,242 oxidized crosslinked preparations, immobilization of proteins by reductive amination, 180,114 Serine carboxypeptidase degradation of peptides, in C-terminal sequence determination with %f plasma desorption mass spectrometry, 180,28 Serine hydroxymethyltransferase, see Glycine hydroxymethyltransferase Serotonin release from platelets, in paf-acether assay by liquid chromatography with electrochemical detection (rabbit), 182,419 Serum, see also Blood, Plasma N-acetylneuraminic acid and mono-0-acetylated derivatives, assay by HPLC with fluorometry (human, rat), 179,162 endogenous lithium levels, electrothermal atomic absorption spectrophotometric assay: clinical applications (human), 182,245 fluorine forms and distribution, analysis (human), 182,371 glucose-containing tetrasaccharide, high-performance liquid affinity chromatography (human), 182,48 hyaluronic acid, enzyme-linked immunosorbent-inhibition assay (human), 178,367 leukotriene B,, negative ion GC-MS assay (human), 179,304 ursodeoxycholic acid, fluorometric assay with bacterial 7P-hydroxysteroid dehydrogenase (human), 179,341 Serum albumin adsorption onto glass surfaces, effect on intensity of circular dichroism spectra (bovine), 177, 178 associated sulfur, GC with flame photometry (human), 178,394 dilute solutions, concentration by electrophoresis, description of apparatus (bovine), 182,315

370

CUMULATIVE

iodination, measurement by iodide microassay (human), 179,138 phospholipase AZ fluorometric assay in presence of, application of pyrene-labeledphospholipids, 177,103 quantitation during purification by fourth-derivative absorption spectroscopy (bovine), 182,141 sequential SDS-PAGE and reverse-phase HPLC (bovine), 178, 385 Sialic acids bound, in sialoglycoproteins, direct acidic ninhydrin assay (mouse), 179,332; erratum, 182,459 Sialoglycoproteins bound sialic acids, direct acidic ninhydrin assay (mouse), 179,332; erratum, 182,459 Silanes thiol-terminal, in antibody immobilization on silica, 178,408 Silica, see Silicon dioxide Silica gel immobilized N-hydroxysuccinimide active ester crosslinking agent and precursors, diffuse reflectance infrared Fourier transform spectroscopy, 176,137 particulates, in solid-phase extraction of DNA, 181,66 polymers, in DNA extraction with phenol:chloroform, 183,13 Silicon dioxide immobilization of antibodies with thiol-terminal silanes and heterobifunctional crosslinkers, 178,408 immobilized membrane lipids, Fourier transform infrared assay: leaching and stability of bonded phases, 182,237 nonporous monodisperse types, 3-isothiocyanatopropyltriethoxysilane-activated, with immobilized ligands, in affinity chromatography, 179,171 octadecyl-bonded, reversible conversion to ion-exchange surfaces for protein separations, 176,191 thiophilic, as adsorbent in one-step HPLC purification of monoclonal antibodies, development, 180,66 Silver staining urease in polyacrylamide gels (Cunaualia ensiformis), 177,37 Slime molds Dictyosteelium discoideum, inositol 1,4,5-trisphosphate levels, isotope dilution assay, 177,115 specific RNA, assay by colony blot technique, 176,78 Sodium acetate “C-labeled, in synthesis of [i4C]acetyl-CoA, 176,82 Sodium borohydride tritiated, reduction of oxidatively modified proteins, in determination of carbonyl groups, 177,419 Sodium chloride role in solubilization and partial purification of hepatic ai-adrenergic receptors by CHAPS (rat), 176,375 Sodium dodecyl sulfate and benzyldimethyl-n-hexadecylammonium chloride, in two-dimensional gel electrophoretic purification of proteins, 176, 457; erratum, 180,194 effects on sensitivity and variability of Bradford protein assay, 178, 263 enhancement of quantitative immunoenzyme dot-blot assays on nitrocellulose, 177,212 and protein solutions, alkyl sulfates in, analysis by isocratic and gradient ion chromatography, 182,103 in two-dimensional gel electrophoresis of actin-binding proteins,

178,32 Sodium dodecyl sulfate-polyacrylamide gels detection of calmodulin-dependent protein kinase II, 183,139 fractionated phosphoproteins, phosphoamino acid analysis, facilitation by acid/base hydrolysis on Immobilon, 176,22 minigel system in peptide mapping by cyanogen bromide fragmentation, 178, 391 separation of biotin-labeled proteoglycans for blotting onto nylon

SUBJECT

INDEX

membranes before and after enzymatic digestion (human aorta), 179,245 proteins electroblotted on polyvinylidene difluoride membranes, amino acid analysis, 181,75 stained with metal salts, Western blots of proteins from, 180,311 ultrathin, immunoblotting of thymus and nuclear extracts by unidirectional diffusion, 176,350 Sodium perchlorate in purification of genomic DNA from whole blood (human), 180, 276 Sodium phosphate role in solubilization and partial purification of hepatic ai-adrenergic receptors by CHAPS (rat), 176,375 Sodium salicylate in tritium Auorography in thin-layer chromatograms and nitrocellulose blots, 178,327 Sodium sulfate evaluation for hydrophobic interaction chromatography in alkaline pH, 182,266 Solutions aqueous and organic, deoxygenation apparatus for transient studies,

180,384 Solvents denaturing, in ion-exchange chromatographic preparation tin depleted of phospholipase A*, 178,118; erratum, Soman low levels, microassay based on acetylcholinesterase

of melit-

181,196 inhibition,

180,380 Somatotropin capillary zone electrophoresis (human), 177,20 recombinant 23-kDa, tryptic digest, mapping by plasma desorption MS (human), 183,190 Sorbitol and myo-inositol in glomerular extract, two-step HPLC (rat kidney), 178,331 Southie blotting technique for cell colony hybridization with DNA probes (murine fibroblasts), 179,217 South Western blot mapping simultaneous characterization of DNA binding proteins and specific genomic DNA target sites (mouse), 179,299 Spectrofluorometry in casein digestion assay (bovine milk), 176,239 dyes in interaction with DNAs of different base composition and conformation, 179,401 monovinyl and divinyl chlorophyll(ide) a and b, in analysis of chloroplast biogenesis (corn, cucumber), 178,294 Spectrophotometry N-acetyl-b-D-glucosaminidase or N-acetyl$-D-hexosaminidase with 3,4-dinitrophenyl N-acetyl-fi-D-glucosaminide, 183, 245 anchorage-dependent cells in tissue culture plates and on microcarriers, 181,209 in assays for plasma paraoxonase/arylesterase hydrolysis of active metabolites of chlorpyrifos and parathion (human), 180,242 catalase, correlation with disk flotation assay (bovine liver), 178, 404 condensing enzyme activity of microsomal fatty acid chain elongation system (rat), 179,251 cyclodextrin glucanotransferase cyclization activity (Bacillus sub-

tilis), 181,6 diacetyl (rat), 180,349 meso-diaminopimelate decarboxylase hydrolase (Cryptococcus laurentii, electrothermal atomic absorption, in levels in serum and urine: clinical 245 with enzymatic amplification, NAD+

and L-cY-amino-t-caprolactam Escherichia coli), 181,297 assay of lithium endogenous applications (human), 182, and NADH,

179,382

CUMULATIVE in estimation of amino groups on polymer supports, 180,253; erratum, 183,197 hydroperoxides assay by iodometric technique, 176,360 continuous-flow automated assay, 176,353 hydroxylated by-products of penicillin V fermentation, application to screening of mutant penicillin producer strains on agar, 179, 288 in indicator assay for amino acid decarboxylases (Escherichia coli), 181,59 indole-3-methanol in small samples withp-dimethylaminocinnamaldehyde, 183,172 -kinetic studies on formation and stability of isoindoles derived from amino acids, o-phthalaldehyde, and N-acetyl-L-cysteine, 180,172 microtiter plate system, assay for low levels of toxic organophosphorus compounds with acetylcholinesterase inhibition, 180,380 with NADPH-linked glutamate dehydrogenase from algae, assay of oxoglutarate and estimation of aminotransferase activities, 180,99 phospholipase A2 hydrolyzing plasmalogens, assay with coupling enzymes, 176,180 ribonuclease inhibitor (porcine brain), 176,175 tryptophan assay in intact proteins by acid ninhydrin method, 177, 16 Spectroscopy, see also specific techniques collagenase assay with peroxidase-labeled collagen, 176,150 in enzymatic assay of mitochondrial tricarboxylate carrier (bovine liver), 177,323 fourth-derivative absorption, in protein quantitation during purification (bovine, Neisseria meningitidis), 182,141 second-derivative absorption, in quantitative analysis of protein mixtures (bovine lens), 181,79 second-derivative ultraviolet, in determination of DNA A-T content, 176,265 Spermatocytes glycosyltransferases, TLC (mouse), 178,421 Spermidine fluorometric assay with electrophoresis on Titan III cellulose acetate (rat tissues, urine), 180,307 Spermine fluorometric assay with electrophoresis on Titan III cellulose acetate (rat tissues, urine), 180,307 Sphingosine enzymatic assay in cultured cells (human, mouse), 183,177 Spinal cord stored at -70°C in ethylene glycol, isolation of motoneuron cell bodies (frog, rat), 180, 286 Spin trapping oxygen-centered free radicals in biological systems, associated problems, review, 177, 1 3(2’-Spiroadamantane)-4-methoxy-4-(3”-phosphoryloxy)phenyl-1,2dioxetane as chemiluminescent substrate in hepatitis B virus DNA hybridization assay, comparison with calorimetric substrate, 180,95 Staining acylphosphatase activity after gel electrophoresis, in analysis of isozyme tissue distribution (chicken, pig), 183,46 background, in Western immunoblotting, temperature-dependent reduction, 177,256 cells cultured on 96well plates, crystal violet assay (human), 182, 16 colloidal gold, see Colloidal gold Coomassie blue, polyacrylamide gels, one-step low-background procedure, 182,157 immuno-, antigens on nitrocellulose, application of oxidative coupling reactions, 183,9

SUBJECT

INDEX

371

immunoblots with peroxidase, subsequent visualization, loo-fold enhancement of sensitivity with ultraviolet light, 182,176 mucus glycoproteins on nitrocellulose membranes, periodate-Schiff and calorimetric assays, 182,160 proteins with copper iodide on nitrocellulose membranes, 181,250 and peptides, with india ink on nitrocellulose, sensitivity improvement by fixation, 174,97; erratum, 178,215 in subnanogram and nanogram range with AuroDye, FerriDye, and India ink, 182,44 pyridine nucleotide-linked dehydrogenases on polyacrylamide gels, 178,243 silver, see Silver staining Stationary phases containing immobilized metal ion, chromatographic separations of proteins (human serum), 183,106 Statistical methods sum-of-squares profile and Monte Carlo method, in evaluation of uncertainties for parameters in binding studies, 176,161 Steroids competitive ELISA, analysis, 183,283 cytochrome P450,, activity, HPLC assay, 182,327 sulfate and glucuronyl conjugates, hydrolysis (equine urine), 182, 289 Storage dried polyacrylamide gels for, preparation, 179,242 Streptavidin -biotin interaction, inhibition by nonfat dried milk, 181,318 detection, application of bioluminescent assays with glucose-6phosphate dehydrogenase, 180,43 in gel retardation assay for estimation of degree of RNA biotinylation, 179,98 Strictosidine synthase from plant cell cultures, assay by HPLC, 176,412 Stromelysin semicontinuous HPLC-based assay, 180,110 Styrofoam modified cup as membrane filter holder for drop dialysis technique using larger volume samples, 179,75 N-Succinimidyl-2-(S)-methoxy-2-phenylacetic acid ester as amine-directed chiral derivatizing reagent suitable for enzymatic-scale resolutions, synthesis. 178,177 o-Succinylbenzoic acid enzymes involved in overall synthesis, HPLC: analysis of vitamin K, biosynthesis (Escherichia coli), 178,306 2-Succinyl-6-hydroxy-2,4-cyclohexadiene-l-carboxylic acid synthase HPLC in analysis of vitamin K2 biosynthesis (Eschwichia coli), 178,306 Sucrose mediated detergent lysis, in preparation of plasmid DNA from gram-positive and -negative bacteria, 176,344 Sugars reducing, fluorescence assay, 177,ll reduction by Nelson-Somogyi assay, adaptation to microtiter platebased microassay, 182,192 residues of complex polysaccharides, absolute configuration, determination by circular dichroism spectroscopy, 179, 209 Sulfamethoxazole hydroxylamine cytotoxicity, evaluation by fluorescence-based viability assay, 177, 364 Sulfatase hydrolysis of sulfoconjugated noradrenaline and dopamine in plasma, effects of ascorbic acid (human), 182,262 Sulfate steroid, hydrolysis (equine urine), 182,289 as substrate in radioactive electrophoretic assay of ATP-sulfurylase in crude extracts, 176,294

372

CUMULATIVE

SUBJECT

Sulfate adenylyltransferase in crude extracts, radioactive electrophoretic assay with sulfate or selenate as substrate, 1’76,294 Sulfhydryl groups alkylation by N-iodoacetyltyramine, in iZ51 labeling of compounds, assay

179,389 in low- and high-molecular-weight

compounds by electron spin resonance (murine, rat blood), 182,58 Sulfobromophthalein %-labeled, of high specific activity, preparation, 179,72 Sulfoethyl aspartamide in ion-exchange chromatography for peptide mapping of Stuphylococcus aureus V8 protein digests, 176,255 Sulfonation DNA, in detection of DNA fragments produced by restriction enzymes, 177,199 Sulfotransferase involved in glucosinolate biosynthesis, radioassay (cabbage), 178, 137 Sulfur protein-associated, GC with flame photometry (human, rat), 178, 394 Sulfuric acid -phenol reaction for estimation of hexoses and pentoses, reevaluation, 181,lS unstable esters, assay for aryl sulfotransferase in associated reactions, evaluation, 183,320 Superose 6 preparative grade column, in fast protein liquid chromatography of plasmid DNA, 177,378 Superoxide anions role in nitroblue tetrazolium chloride reduction mediated by NADH andphenazine methosulfate, 176,170 Superoxide dismutase in tissue homogenates, nitroblue tetrazolium-based assay (rat mammary gland), 179,s supports solid, chemistry of cleavable assayable linking molecules: definition of chemical events and ligand titers, 181,242 Sweat glands eccrine, glycogen-free viable, preparation, application of NADHlinked luciferase assay for glycogen (human), 176,228 Synaptosomes release of [y-3H]aminobutyric acid in uitro, measurement by superfusion system on subsecond time scale (rat brain),

178,s Synchronization large-scare, cultured L1210 cells with aphidicolin, study quent cell cycle regulation of thymidylate synthase drofolate reductase (mouse), 182,338 Synovial fluid hyaluronic acid, enzyme-linked immunosorbent-inhibition (human), 178,367

of subseand dihy-

assay

T Tabun low

levels,

microassay

based

on acetylcholinesterase

inhibition,

180,380 T4 DNA ligase based synthesis of DNA probes complementary to variable number tandem repeat locus (human), 179,280 Temperature incubation, effect on background staining of Western blots with monoclonal antibodies, 177,256 Testis cytochrome c, assay in tissues from developing rats, 181,140

INDEX

Tetracycline biosynthesis, kinetics tion reverse-phase

of terminal reactions, HPLC micromethod

analysis by direct-injec(Streptomyces aureofu-

ciens), 181,125 Thin-layer chromatography glycosyltransferases in spermatogenic cells (mouse), 178,421 inositol phosphates, review, 183,199 phosphotyrosine, phosphoserine, and phosphothreonine in protein hydrolysate, 177,138 [%]sulfobromophthalein of high specific activity, 179,72 tritium-labeled palmitic acid, subsequent sodium salicylate-based Auorographic detection, 178,327 Thioacetylation based method for protein sequencing: derivatization of L-methyl5(4H)-thiazolones for HPLC, 181,113 Thiobarbituric acid reactive substances in oxidized lipids, assay by HPLC with postcolumn reaction system (rat liver), 182,116 Thiocyanate voltammetric assay in small biological samples (human blood, tissues), 181,190 Thioflavine T in fluorometric assay of amyloid fibrils in vitro (murine liver), 177, 244 Thiols protein and small-molecular-weight, selective reactions with 1-p-chlorophenyl-4,4-dimethyl-5-diethylamino-1 -penten-3-one hydrobromide, characterization, 177,237 Thymidine kinase assay based on precipitation of nucleoside monophosphates with lanthanum chloride, 178,38 Thymidylate synthase cell cycle regulation after large-scale synchronization of cell cultures with aphidicolin (murine leukemia cells), 182,338 inhibited cells, dUTP radioimmunoassay in extracts (human lung carcinoma cells), 177,347 Thymine and uracil dimers in DNA, assay by HPLC/GC with electron capture detection, 182,424 Thyroxine catalytic activities, measurement by iodide microassay, 179,138 homogeneous enzyme immunoassay with reversed micellar systems,

181,145 Tissues acylphosphatase isozyme distribution, analysis by activity staining after gel electrophoresis (chicken, pig), 183,46 aldose reductase immunoquantitation (human), 176,66 choline and metabolite levels, HPLC and GC-MS assay (rat), 180, 85 cultured attached cells in culture plates and on microcarriers, colorimetric assay, 181,209 cyanide and thiocyanate, voltammetric assay in small samples (human), 181,190 desferrioxamine-available iron, reverse-phase HPLC assay with uv detection (rabbit), 180,126 freeze-clamped, homogenization preparation technique for metaboIite analyses (rat heart), 181,239 glucose oxidase, electrochemical quantitation (mouse), 178,427 green plant, protein extraction for two-dimensional electrophoresis,

177,33 polyamines, fluorometric assay with electrophoresis on Titan III cellulose acetate (rat), 180,307 printing on nitrocellulose, in histochemical localization of cysteinerich proteins (barley), 182,334 protein-associated sulfur, GC with flame photometry (rat), 178, 394 skeletal, total cellular RNA, isolation and analysis (rat), 183,301

CUMULATIVE testicular and somatic, from developing rats, associated cytochromes c, assay, 181,140 Titration active-site, hydrolases, oxazolones as substrates, 181,321 low-temperature electron paramagnetic resonance, oxygen-sensitive proteins, description of spectroelectrochemical cell, 181, 283 Tocopherols and tocotrienol, isomers, normal and reverse-phase HPLC, 180, 368 Tocotrienol and tocopherols, isomers, normal and reverse-phase HPLC, 180, 368 Toxins entomocidal, assay of C-terminus by diagonal electrophoresis (Ba-

cillus thuringiensis),

181,212

Transblotting in identification of pyruvate carboxylase and methylcrotonoyl-CoA carboxylase (rat liver), 177,57 Transfection efficiency, role in variation in enzymatic transient gene expression assays (mouse), 182,411 mammalian cell lines by electroporation, optimization, 180,269 Transferrins galactosylation variants, immunofluorometric assay with immobilized Bandeiraea simplicifolia II lectin, 177,383 Transforming growth factor type n, transferred to nylon membranes for Western blot analysis, increase of retention by glutaraldehyde fixation, 178,255 Transglutaminase, see Protein-glutamine y-glutamyltransferase Translation nick, see Nick translation Trehalase stable preparation, in determination of trehalose in biological samples, 176,432 Trehalose determination in biological samples by stable trehalase preparation,

176,432 Triacylglycerol lipase nanomole quantities, assay by s3Ni binding to fatty acid, 178,413 Tri-L-alanine immobilized on 3-isothiocyanatopropyltriethoxysilane-activated nonporous monodisperse silicas, affinity chromatography, 179, 171 Tributyl phosphine reduction of cyst(e)ine-containing proteins prior to structural anal-

yses,180,231 Tricarboxylate carrier mitochondrial, reconstitution and spectroscopic enzymatic assay (bovine liver), 177,323 Tricyclic nucleoside-5’-monophosphate FAB/MS, low-nanogram detection, 178, 102 Triiodothyronine rhodamine B-labeled, formation of high-molecular-weight aggregates in solution: possible source of artifacts in binding experiments, 176,440 Trimetrexate plasma levels in cancer patients, fluorescein-methotrexate-based flow cytometric bioassay, 178,287 2,3,5-Triphenyltetrazolium chloride direct detection of P-1,3-glucanase isozymes on polyacrylamide electrophoresis and isoelectrofocusing gels (tobacco), 182,136 Tritium release, in purine-nucleoside phosphorylase isotopic assay, 183, 279 sodium salicylate-based fluorographic detection in thin-layer chromatograms and nitrocellose blots, 178,327

SUBJECT

INDEX

373

Triton X-100 -acetic acid-urea, in protein gel electrophoresis after two-dimensional gel electrophoresis, 177,388 effects on sensitivity and variability of Bradford protein assay, 178, 263 interference in Lowry protein assay, elimination procedure, 183, 275 tRNA sulfurtransferase DEAE-cellulose filter disk assay (Eschrichia coli), 176,57 Trypsin active-site titrations, oxazolones as substrates, 181,321 detection with self-quenched fluorogenic protein substrates, 176, 261 digested 23-kDa recombinant human growth hormone, fragment mapping by plasma desorption MS, 183,190 fluorescent oligopeptide energy transfer assay (bovine pancreas),

183,50 inhibition assay, role of limited hydrolysis of inhibitors, 178,159 and trypsin-like enzymes, radioassay, 179,56 Trypsin inhibitors limited hydrolysis, role in trypsin inhibition assay, 178,159 Trypsinogen activation by enterokinase, size-exclusion HPLC assay (bovine),

176,132 Tryptophan deuterium-labeled, HPLC-thermospray MS in proteins (Halobacterium hulobium), 181,271 in intact proteins, spectrophotometric assay by acid ninhydrin method, 177,16 Tubule cells distal and proximal, isolation from renal cortex and efficacy in study of chemical-induced cytotoxicity (rat), 182,271 Tumor cells breast cancer protein-tyrosine kinase, nonradioactive dot-blot assay (human),

182,98 transfected, chloramphenicol acetyltransferase activity, mixedphase assay (human), 179,19 ductal carcinoma, giycohistochemistry, application of neoglycoenzyme (human), 182,447 electrotransfection parameters, testing with Lucifer yellow CH (human), 181,309 glioma, malignant, DNA single-strand breaks, fast components of rejoining kinetics, derivative assay (human), 182,233 intestinal adenocarcinoma, small numbers, estimation by ELISA (human), 177,259 leukemia electroporation with nonuniform electrical field, cylinder design (mouse), 182,253 large-scale synchronization with aphidicolin: study of cell cycle regulation of thymidylate synthase and dihydrofolate reductase (mouse), 182,338 purine precursors, chromatographic assay, 182,121 lung carcinoma, thymidylate synthase-inhibited, dUTP radioimmunoassay in extracts (human), 177,347 Tyramine dopamineand norepinephrine-related, assay by prechromatographic derivatization with naphthalene-2,3-dicarboxaldehyde (human urine), 180,279 Tyrosine residues, in proteins and peptides modified by arsanilic acid, identification, 177,183 Tyrosine decarboxylase apoenzyme, streptococcal, in HPLC assay of plasma pyridoxal 5’phosphate (human), 181,267 Tyrosine hydroxylase spot-blot immunoassay with affinity-purified antibodies (bovine, rat), 181,259

374

CUMULATIVE

Tyrosyl radicals scavenging activity of antioxidants, say: ovothiol A identification

qualitative fluorometric asas efficient scavenger, 180,326

U UDP-galactose bioluminescent microanalysis in milk and plasma (human), 176, 449 UDP-glucose bioluminescent microanalysis in milk and plasma (human), 176, 449 Ultracentrifugation air-driven, for replacement of Golgi vesicle intravesicular contents (rat liver), 180,216 associated digital data acquisition system, description, 180,319 Ultrafiltration tangential flow, in large-scale preparation of bacteriophage X for isolation of DNA, 176,373 Ultraviolet detection chitinase after PAGE, 178,362 with HPLC in assay of cytochrome P450,, activity, 182,327 hopanoids in bacterial cell cultures, 181,120 with reverse-phase HPLC, desferrioxamine-available iron in tissues (rabbit), 180, 126 Ultraviolet light absorbance with diaminobenzoic acid fluorescence assay, in quantifying and ascertaining purity of DNA preparation (maize), 180,314 irradiation of deoxyribonucleoprotein, subsequent isolation of modified histone H3 by reverse-phase HPLC, 178,88 100-fold enhancement of sensitivity in visualization of peroxidasestained blots, 182, 176 Ultraviolet shadowing nucleic acids on nylon membranes, 178,41 Uracil and thymine dimers in DNA, assay by HPLC/GC with electron capture detection, 182,424 Urate oxidase immobilized, and chemiluminescence detection, in flow injection assay of inorganic phosphate, 182,366 in plasma, radiochemical assay with HPLC (human), 176,290 Urea -acetic acid-Triton X-100, in protein gel electrophoresis after twodimensional gel electrophoresis, 177,388 denaturation of 20fi-hydroxysteroid dehydrogenase, and enzyme renaturation, size-exclusion chromatographic analysis, 180, 181 effects on sensitivity and variability of Bradford protein assay, 178, 263 Urease selective silver staining in polyacrylamide gels (Canauolia ensi-

SUBJECT

INDEX

homocysteine and penicillamine and related symmetrical and mixed disulfides, reverse-phase HPLC with electrochemical detection (human), 180,259 polyamines, fluorometric assay with electrophoresis on Titan III cellulose acetate (rat), 180,307 steroid sulfate and glucuronyl conjugates, hydrolysis (horse), 182, 289 zinc protoporphyrin and porphyrin carboxylic acids, simultaneous assay by reverse-phase HPLC with fluorometry (human), 183, 134 Urokinase, see Plasminogen activator Uroporphyrinogen-I synthase in Ampholine pH gradient, preparative free solution isoelectric focusing (human erythrocytes), 182,217 Ursodeoxycholic acid serum, fluorometric assay with bacterial 7@-hydroxysteroid dehydrogenase (human), 179,341

V Vasopressin immunodetection on nitrocellulose, 183,250 Vesicles Golgi membrane, contents, method for replacement with air-driven ultracentrifuge (rat liver), 180, 216 outer membrane, quantitation during purification by fourth-derivative absorption spectroscopy (Neisseria meningitidis), 182,141 pinocytic, of defined age, magnetic separation (Entamoeba histolytica), 182,77 Viability assay fluorescence-based, for studies of reactive drug intermediates, 177, 364 in mammalian cell systems, review, 179, 1 Victoria blue types B and R, in assay of phospholipids (Micrococcw lysodeikticus),

180,186 Vitamin K1 dependent carboxylase, assay accuracy: effects of minimized escape of CO2 from solution on reaction rate linearity, 182,207 Vitamin K, biosynthesis, HPLC assay of enzymes involved in o-succinylbenzoic acid synthesis (Escherichia coli), 178,306 Voltammetry cyanide and thiocyanate in small biological samples (human blood, tissues), 181,190

vx low

levels,

microassay

based

on acetylcholinesterase

inhibition,

180,380 W

formis),177,37 Urine acylcarnitines, assay by FAB/MS and liquid chromatography/MS (human), 180,331 bound selenomethionine, trace levels, indirect determination (human), 178,77 5,6-dihydrouridine, identification by GS-MS (human), 181, 302 endogenous lithium levels, electrothermal atomic absorption spectrophotometric assay: clinical applications (human), 182,245 ethyl alcohol, enzymatic assays with superabsorbent polymers (human), 182,257 free and acylcarnitine levels, determination by FAB/MS (human), 176,85 glucose-containing tetrasaccharide, high-performance liquid affinity chromatography (human), 182,48

Warfarin oxidative metabolites, thermospray HPLC with MS, 177,307 Water -t-butanol-ammonium sulfate, in three-phase partitioning isolation of cathepsin D (bovine spleen), 180,169 Western blotting with affinity-purified antibodies, in tyrosine hydroxylase assay (bovine, rat), 181,259 low-molecular-weight growth factors on nylon membranes, increase of retention by glutaraldehyde fixation, 178,255 proteins background staining with monoclonal antibodies, temperaturedependent reduction (human milk), 177,256 from Coomassie blue-stained polyacrylamide gels, in luminescent immunodetection technique, 179,206

CUMULATIVE

SUBJECT

on nitrocellulose, and gold staining, subsequent artifact appearance, 180,55 from SDS-polyacrylamide gels stained by metal salts, 180,311 Wheat germ agglutinin linked to microtiter plates, directional immobilization of Na,KATPase, 180,362 X Xanthine oxidase immobilized, and chemiluminescence detection, say of inorganic phosphate, 182,366 Xenobiotics cysteine, N-acetylcysteine, and glutathione analysis by FAB and tandem MS, 183,94

375

INDEX

phosphatidylserine decarboxylase, DEAE-cellulose chromatographic assay, 182,452 unspheroplasted, preparation of RNA by homogenization in hightemperature phenobchloroform mixtures, 176,303 viable cell density, chemiluminescent assay, 181,149 specific RNA, application of colony blot technique, 176,78 Z

in flow injection

adducts,

as-

structural

interaction immobilized Zinc chloride stained proteins

with metal

peptides, ion affinity

on SDS-polyacrylamide

evaluation by high-performance chromatography, 183,159 gels, Western

blots

from,

180,311

Y Yeasts DNA isolation, 178,82 Saccharomyces cerevisiae cells with ampicillin resistance gene in chimeric metric detection of p-lactamase, 177,165

Zinc ionic,

plasmids,

iodo-

Zinc protoporphyrin and porphyrin carboxylic acids, simultaneous assay in urine by reverse-phase HPLC with Huorometry (human), 183,134 Zymogram method application to DNase I after isoelectric focusing: enzyme genetic analysis (human urine), 183,84