- Email: [email protected]
Cystic Lesion in the Adrenal Gland of a Beagle Dog
J. Comp. Path. 2017, Vol. 156, 54e141
ESVP and ECVP Proceedings 2016
131
RECURRENT MALIGNANT PERIPHERAL NERVE SHEATH TUMOUR IN AN AFRICAN HEDGEHOG (ATELERIX ALBIVENTRIS) J.G. Xavier *,y, F.B. Santos y, B.F. Monteiro y, P.M.C. Souza y and M.A. Lallo* *Environmental and Experimental Pathology Post-Graduation, Paulista University and yEscola de Ci^encias Medicas e da Saude, Universidade Metodista de S~ao Paulo, Brazil Introduction: Soft tissue sarcomas are a heterogeneous population of mesenchymal tumours arising preferentially in middle to older aged animals. Malignant peripheral nerve sheath tumours (MPNSTs) arise from Schwann cells, perineurial fibroblasts, or both. Here we describe the histological and immunohistochemical features of a recurrent MPNST in a hedgehog. Materials and Methods: A 3-year-old female African hedgehog (Atelerix albiventris) developed a recurrent mass in the vagina. Tissue samples were collected, fixed in 10% neutral buffered formalin, processed routinely and embedded in paraffin wax. Sections (4 mm) were stained with haematoxylin and eosin. An immunohistochemical panel consisting of vimentin, a-smooth muscle actin (a-SMA), glial fibrillary acid protein (GFAP), neuron-specific enolase, CD10 and S100 protein was applied. Results: The histopathological findings were high cellularity, with variably pleomorphic, plump spindle cells arranged mostly in interlacing bundles, with bizarre mitotic figures. Areas of palisading and whorls were observed. The tumour cells were positive for vimentin, enolase, S100 and CD10 with negativity for a-SMA and GFAP. The morphological and immunohistochemical characteristics were consistent with the diagnosis of MPNST. Despite the high number of tumours in hedgehogs, this is only the second report of a vaginal MPNST tumor in an African hedgehog. Conclusions: The histogenesis of soft tissue tumours can be difficult to determine on the basis of routine histological analysis. Immunohistochemistry is a complementary method for the differential diagnosis of MPNST and we have shown cross-reactivity of some human antibodies with hedgehog tissues.
URACHAL CARCINOMA IN A YOUNG ADULT DOG M. Rondena *, C. Giudice y, D. Stefanello y, D. Zani y, V. Giacobbi * and V. Baldassarre* *Laboratorio d’Analisi Veterinarie San Marco, Padova and yDepartement of Veterinary Medicine, University of Milan, Milano, Italy Introduction: Tumours arising from embryonal remnants are rare; among these urachal carcinoma (UC) is reported sporadically in man, mostly in adult and aged patients, and less frequently in children and adolescents. To our knowledge UC has never been reported in dogs. Materials and Methods: An 18-month-old, male, Labrador retriever dog was referred for abdominal pain. Ultrasonography revealed multiple large (up to 25 cm) cystic abdominal masses, adherent to the omentum and close to the pancreas and spleen. The largest mass was removed surgically and formalin fixed for histology. Immunohistochemistry with antibodies against cytokeratin and uroplakin III was also performed. Computed tomography examination did not reveal findings compatible with metastatic lesions. A postoperative change in chemotherapy was performed. Results: Histologically, a large, multinodular, partially encapsulated, infiltrating, cystic neoplasm, involving mesenteric fat was observed. The neoplasm was composed of small cystic structures lined either by simple columnar epithelium with intermingled goblet cells (intestinal type) or by pseudostratified epithelium with 4e5 rows of nuclei and occasionally superficial luminal ‘umbrella’ cells (urothelial type). Immunohistochemically, all neoplastic cells were intensely and diffusely cytokeratin positive. In urothelial type areas, neoplastic cells also showed intense intracytoplasmic expression of uroplakin III. Twelve months after surgery, the dog is still alive and in very good clinical condition without relapse. Conclusions: UC is rare in human medicine and is characterized by double intestinal (colonic) and urothelial differentiation. Metastatic cases are reported, with variable fates. The present case is the first report of UC in dogs. The diagnosis was possible based on both histology and immunohistochemistry.
CYSTIC LESION IN THE ADRENAL GLAND OF A BEAGLE DOG E. Riccardi *, G. Di Gallo y, F. Marchesi z, A.M. Giusti x, P. Roccabianca { and J. McKay* *IDEXX Laboratories, Wetherby, UK, yAccelera Srl, Nerviano, Italy, zSchool of Veterinary Medicine, University of Glasgow, UK, xRoche Innovation Center, Z€urich, Switzerland and {Department of Veterinary Sciences and Public Health, University of Milan, Italy Introduction: Cystic lesions of the adrenal gland are uncommon in man and only a few cases have been reported. In domestic animals, the occurrence of epithelial cysts has been reported in the adrenal glands of non-human primates, but never reported in the dog. Materials and Methods: In the context of a chronic repeat dose toxicity study, a single spherical cystic lesion, 1 mm in diameter, was observed in the cortex of one adrenal gland from a male beagle dog. No clinical signs or other relevant gross changes were recorded for this dog. Histological analysis, special stains and immunohistochemical analysis were performed in order to characterize the lesion. Results: Histologically, the cyst was lined by a stratified epithelium composed of cuboidal to columnar cells, occasionally vacuolated. The cyst was filled by moderate amounts of pale eosinophilic amorphous material and red blood cells. PAS and alcian blue-positive material was present in the cytoplasmic vacuoles. Epithelial cells expressed CK19 and CK20. Conclusions: The histological, histochemical and immunohistochemical analysis suggested an intestinal origin of this adrenal cyst. The lesion was interpreted as an intestinal choristoma. The occurrence of intestinal choristoma in the dog has been reported as a single instance only in the subcutis of the flank.
A CASE REPORT OF HEPATIC MYELOLIPOMA IN A FERRET (MUSTELA PUTORIUS FURO) E.E. Scaglione *, L. Starvaggi Cucuzza *, A. Sereno *, M. Ferri y and E. Bollo* *Department of Veterinary Science, University of Turin and yVeterinary Practitioner, Italy Introduction: Myelolipoma is a rare benign mesenchymal tumour primarily composed of mature adipose tissue with scattered haemopoietic elements in different proportions. Myelipomas are typically non-functioning tumours and are therefore often asymptomatic. Several cases of myelolipoma have been reported in man and animals, but only a splenic myelolipoma has been reported in the ferret. Materials and Methods: A 6-year-old neutered male white ferret (Mustela putorius furo) was referred with sudden depression and anorexia. Abdominal ultrasonography revealed two hepatic masses, which were removed surgically. Specimens were fixed in 10% phosphate-buffered formalin for histological examination. Results: Histologically, the mass showed mature adipocytes associated with haemopoietic elements, represented by granulocytic, erythrocytic and megakaryocytic series at different stages of maturation. These findings were consistent with a diagnosis of myelolipoma. This is the first report of a hepatic myelolipoma in a ferret. Conclusions: The pathogenesis of myelolipoma remains unclear: it is considered to be a hormonally induced tumour of stromal cells or primitive mesenchymal cells. Another hypothesis is related to a possible activation of dormant haemopoietic stem cells in the peritoneum that had been active during embryonic development. Myelolipomas are also speculated to be derived from bone marrow emboli lodging in different organs. Others authors suggest that myelolipoma is a choristoma, arising from normal haemopoietic stem cells, misplaced during embryogenesis. The few case reports in veterinary literature suggest that myelolipoma is a disease of the geriatric animal, with a similar scenario to that reported in man.
ESVP and ECVP Proceedings 2016
131
RECURRENT MALIGNANT PERIPHERAL NERVE SHEATH TUMOUR IN AN AFRICAN HEDGEHOG (ATELERIX ALBIVENTRIS) J.G. Xavier *,y, F.B. Santos y, B.F. Monteiro y, P.M.C. Souza y and M.A. Lallo* *Environmental and Experimental Pathology Post-Graduation, Paulista University and yEscola de Ci^encias Medicas e da Saude, Universidade Metodista de S~ao Paulo, Brazil Introduction: Soft tissue sarcomas are a heterogeneous population of mesenchymal tumours arising preferentially in middle to older aged animals. Malignant peripheral nerve sheath tumours (MPNSTs) arise from Schwann cells, perineurial fibroblasts, or both. Here we describe the histological and immunohistochemical features of a recurrent MPNST in a hedgehog. Materials and Methods: A 3-year-old female African hedgehog (Atelerix albiventris) developed a recurrent mass in the vagina. Tissue samples were collected, fixed in 10% neutral buffered formalin, processed routinely and embedded in paraffin wax. Sections (4 mm) were stained with haematoxylin and eosin. An immunohistochemical panel consisting of vimentin, a-smooth muscle actin (a-SMA), glial fibrillary acid protein (GFAP), neuron-specific enolase, CD10 and S100 protein was applied. Results: The histopathological findings were high cellularity, with variably pleomorphic, plump spindle cells arranged mostly in interlacing bundles, with bizarre mitotic figures. Areas of palisading and whorls were observed. The tumour cells were positive for vimentin, enolase, S100 and CD10 with negativity for a-SMA and GFAP. The morphological and immunohistochemical characteristics were consistent with the diagnosis of MPNST. Despite the high number of tumours in hedgehogs, this is only the second report of a vaginal MPNST tumor in an African hedgehog. Conclusions: The histogenesis of soft tissue tumours can be difficult to determine on the basis of routine histological analysis. Immunohistochemistry is a complementary method for the differential diagnosis of MPNST and we have shown cross-reactivity of some human antibodies with hedgehog tissues.
URACHAL CARCINOMA IN A YOUNG ADULT DOG M. Rondena *, C. Giudice y, D. Stefanello y, D. Zani y, V. Giacobbi * and V. Baldassarre* *Laboratorio d’Analisi Veterinarie San Marco, Padova and yDepartement of Veterinary Medicine, University of Milan, Milano, Italy Introduction: Tumours arising from embryonal remnants are rare; among these urachal carcinoma (UC) is reported sporadically in man, mostly in adult and aged patients, and less frequently in children and adolescents. To our knowledge UC has never been reported in dogs. Materials and Methods: An 18-month-old, male, Labrador retriever dog was referred for abdominal pain. Ultrasonography revealed multiple large (up to 25 cm) cystic abdominal masses, adherent to the omentum and close to the pancreas and spleen. The largest mass was removed surgically and formalin fixed for histology. Immunohistochemistry with antibodies against cytokeratin and uroplakin III was also performed. Computed tomography examination did not reveal findings compatible with metastatic lesions. A postoperative change in chemotherapy was performed. Results: Histologically, a large, multinodular, partially encapsulated, infiltrating, cystic neoplasm, involving mesenteric fat was observed. The neoplasm was composed of small cystic structures lined either by simple columnar epithelium with intermingled goblet cells (intestinal type) or by pseudostratified epithelium with 4e5 rows of nuclei and occasionally superficial luminal ‘umbrella’ cells (urothelial type). Immunohistochemically, all neoplastic cells were intensely and diffusely cytokeratin positive. In urothelial type areas, neoplastic cells also showed intense intracytoplasmic expression of uroplakin III. Twelve months after surgery, the dog is still alive and in very good clinical condition without relapse. Conclusions: UC is rare in human medicine and is characterized by double intestinal (colonic) and urothelial differentiation. Metastatic cases are reported, with variable fates. The present case is the first report of UC in dogs. The diagnosis was possible based on both histology and immunohistochemistry.
CYSTIC LESION IN THE ADRENAL GLAND OF A BEAGLE DOG E. Riccardi *, G. Di Gallo y, F. Marchesi z, A.M. Giusti x, P. Roccabianca { and J. McKay* *IDEXX Laboratories, Wetherby, UK, yAccelera Srl, Nerviano, Italy, zSchool of Veterinary Medicine, University of Glasgow, UK, xRoche Innovation Center, Z€urich, Switzerland and {Department of Veterinary Sciences and Public Health, University of Milan, Italy Introduction: Cystic lesions of the adrenal gland are uncommon in man and only a few cases have been reported. In domestic animals, the occurrence of epithelial cysts has been reported in the adrenal glands of non-human primates, but never reported in the dog. Materials and Methods: In the context of a chronic repeat dose toxicity study, a single spherical cystic lesion, 1 mm in diameter, was observed in the cortex of one adrenal gland from a male beagle dog. No clinical signs or other relevant gross changes were recorded for this dog. Histological analysis, special stains and immunohistochemical analysis were performed in order to characterize the lesion. Results: Histologically, the cyst was lined by a stratified epithelium composed of cuboidal to columnar cells, occasionally vacuolated. The cyst was filled by moderate amounts of pale eosinophilic amorphous material and red blood cells. PAS and alcian blue-positive material was present in the cytoplasmic vacuoles. Epithelial cells expressed CK19 and CK20. Conclusions: The histological, histochemical and immunohistochemical analysis suggested an intestinal origin of this adrenal cyst. The lesion was interpreted as an intestinal choristoma. The occurrence of intestinal choristoma in the dog has been reported as a single instance only in the subcutis of the flank.
A CASE REPORT OF HEPATIC MYELOLIPOMA IN A FERRET (MUSTELA PUTORIUS FURO) E.E. Scaglione *, L. Starvaggi Cucuzza *, A. Sereno *, M. Ferri y and E. Bollo* *Department of Veterinary Science, University of Turin and yVeterinary Practitioner, Italy Introduction: Myelolipoma is a rare benign mesenchymal tumour primarily composed of mature adipose tissue with scattered haemopoietic elements in different proportions. Myelipomas are typically non-functioning tumours and are therefore often asymptomatic. Several cases of myelolipoma have been reported in man and animals, but only a splenic myelolipoma has been reported in the ferret. Materials and Methods: A 6-year-old neutered male white ferret (Mustela putorius furo) was referred with sudden depression and anorexia. Abdominal ultrasonography revealed two hepatic masses, which were removed surgically. Specimens were fixed in 10% phosphate-buffered formalin for histological examination. Results: Histologically, the mass showed mature adipocytes associated with haemopoietic elements, represented by granulocytic, erythrocytic and megakaryocytic series at different stages of maturation. These findings were consistent with a diagnosis of myelolipoma. This is the first report of a hepatic myelolipoma in a ferret. Conclusions: The pathogenesis of myelolipoma remains unclear: it is considered to be a hormonally induced tumour of stromal cells or primitive mesenchymal cells. Another hypothesis is related to a possible activation of dormant haemopoietic stem cells in the peritoneum that had been active during embryonic development. Myelolipomas are also speculated to be derived from bone marrow emboli lodging in different organs. Others authors suggest that myelolipoma is a choristoma, arising from normal haemopoietic stem cells, misplaced during embryogenesis. The few case reports in veterinary literature suggest that myelolipoma is a disease of the geriatric animal, with a similar scenario to that reported in man.