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Cytogenetic analysis of follicular and papillary thyroid tumors
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Abstracts n o n - r a n d o m events a s s o c i a t e d with UM. These changes are d i s t i n c t from those a s s o c i a t e d with cutaneous malignant melanoma. Molecular investigations are r e q u i r e d to further elucidate the genetic a l t e r a t i o n s at these sites w h i c h may c o n t r i b u t e to the i n i t i a t i o n and p r o g r e s s i o n of urea1 melanoma.
B 1 3 LOSS OF HETEROZYGOSITY STUDIES IN THYROID NEOPLASIA Marie A. Herrmann, Steven R. Ritland, Ian D. Hay and Robert B. Jenkins. Mayo Clinic, Rochester, MN. Consistent loss of hem-ozygosity (LOH) of specific chromosome loci may suggest the presence of recessive oncogenes important for the development or pro~'ession of particular tumors. C y ~ genetic studies of papillary and follicular carcinomas, the most frequently occurring primary thyroid cancers, have shown abnormalities of chromosomes I0 and 3, respectively, and a papillary thyroid carcinoma (PTC) oncogene has been identified on chromosome 10q. Using ~ight DNA probes specific for chromosome 3 and nine DNA probes specific for chromosome I0 we have carried out restriction fragment length polymorphism analysis on tumors from 6 patients with follicular carcinoma, 3 patients with follicular adenoma, and 12 patients with papillary carcinoma. Single DNA probes for loci on chromosomes I, 16 and 17 were used as controls since cytogenetic abnormalities of these chromosomes are infrequent in thyroid cancer. LOH of all informative loci on chromosome 3 was fotmd in all six follicular carcinomas; in addition, two follicular carcinomas had LOH of loci on chromosome 10, and one had LOH of loci on chromosomes 10, 16 and 17. No LOH was observed for loci mapped to chromosomes 1, 3, 10, 16 or 17 in the follicular adenomas or papillary carcinomas, with the exception of one adenoma with loss of a locus on 16p. Our results suggest that there is a recessive oncogene present on chromosome 3 important for the development or progression of follicular thyroid carcinoma.
B 14
CYTOGENETIC ANALYSIS OF FOLLICULAR AND PAPILLARY TnX~0ID TUMORS. R.R. Schreck, T. Ross, J.A. FaEin, Cedars-Sinal Medical Center, Los Angeles, CA.
Cytogenetic analysis of tu~ors has proven to be a valuable ray of i d e n t i f y i n g the l o c a t i o n of genes involved in the development of aaliEnancy. Specific types o f ~ r o m o s o m e aberrations ~ v e been ~ s o c i a ~ e d v ~ h s p e c i f i c ~ene a l t e r a t i o n s (e.g., ~r~slocat~ons vi~h E ~ e a r r o g a t e s in ~ L ~ d deletions v ~ h ~he loss of ~ o r re~nobl~o~ ~d ~tlm's ~or).
B u r k ~ ' s lympho~, suppressor genes in ~ l~t~le ts
c u r r ~ t l y ~ o ~ abou~ ~he E ~ e ~ i c ~ e s involved ~n ~he me~ism of ~hyroid c~cer, cy~ogenetic invest~gation of ~hese ~ o r s should provide valuable ~nfo~lon. To da~e, we ~ v e ~ l n ~ nine ~hyro~d ~umors, s~x f o l l i c u l a r a d e n o ~ s , ~ d ~hree p a p i l l a r y
Abstracts n o n - r a n d o m events a s s o c i a t e d with UM. These changes are d i s t i n c t from those a s s o c i a t e d with cutaneous malignant melanoma. Molecular investigations are r e q u i r e d to further elucidate the genetic a l t e r a t i o n s at these sites w h i c h may c o n t r i b u t e to the i n i t i a t i o n and p r o g r e s s i o n of urea1 melanoma.
B 1 3 LOSS OF HETEROZYGOSITY STUDIES IN THYROID NEOPLASIA Marie A. Herrmann, Steven R. Ritland, Ian D. Hay and Robert B. Jenkins. Mayo Clinic, Rochester, MN. Consistent loss of hem-ozygosity (LOH) of specific chromosome loci may suggest the presence of recessive oncogenes important for the development or pro~'ession of particular tumors. C y ~ genetic studies of papillary and follicular carcinomas, the most frequently occurring primary thyroid cancers, have shown abnormalities of chromosomes I0 and 3, respectively, and a papillary thyroid carcinoma (PTC) oncogene has been identified on chromosome 10q. Using ~ight DNA probes specific for chromosome 3 and nine DNA probes specific for chromosome I0 we have carried out restriction fragment length polymorphism analysis on tumors from 6 patients with follicular carcinoma, 3 patients with follicular adenoma, and 12 patients with papillary carcinoma. Single DNA probes for loci on chromosomes I, 16 and 17 were used as controls since cytogenetic abnormalities of these chromosomes are infrequent in thyroid cancer. LOH of all informative loci on chromosome 3 was fotmd in all six follicular carcinomas; in addition, two follicular carcinomas had LOH of loci on chromosome 10, and one had LOH of loci on chromosomes 10, 16 and 17. No LOH was observed for loci mapped to chromosomes 1, 3, 10, 16 or 17 in the follicular adenomas or papillary carcinomas, with the exception of one adenoma with loss of a locus on 16p. Our results suggest that there is a recessive oncogene present on chromosome 3 important for the development or progression of follicular thyroid carcinoma.
B 14
CYTOGENETIC ANALYSIS OF FOLLICULAR AND PAPILLARY TnX~0ID TUMORS. R.R. Schreck, T. Ross, J.A. FaEin, Cedars-Sinal Medical Center, Los Angeles, CA.
Cytogenetic analysis of tu~ors has proven to be a valuable ray of i d e n t i f y i n g the l o c a t i o n of genes involved in the development of aaliEnancy. Specific types o f ~ r o m o s o m e aberrations ~ v e been ~ s o c i a ~ e d v ~ h s p e c i f i c ~ene a l t e r a t i o n s (e.g., ~r~slocat~ons vi~h E ~ e a r r o g a t e s in ~ L ~ d deletions v ~ h ~he loss of ~ o r re~nobl~o~ ~d ~tlm's ~or).
B u r k ~ ' s lympho~, suppressor genes in ~ l~t~le ts
c u r r ~ t l y ~ o ~ abou~ ~he E ~ e ~ i c ~ e s involved ~n ~he me~ism of ~hyroid c~cer, cy~ogenetic invest~gation of ~hese ~ o r s should provide valuable ~nfo~lon. To da~e, we ~ v e ~ l n ~ nine ~hyro~d ~umors, s~x f o l l i c u l a r a d e n o ~ s , ~ d ~hree p a p i l l a r y