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Cytogenetic effect of uranyl nitrate and cadmium chloride on V79 Chinese hamster cells
288 2.9%). The frequency of chromosome aberrations detected in the mouse bone marrow cells and in spermatogonia as well as the number of detected sperm abnormalities give a clear-cut evidence that ECHH was mutagenic for mice at 5 mg/m 3. Vachkova-Petrova, R., Institute of Nutrition, Sofia, Bulgaria
Mutagenicity study of endodan in rats Endodan, etem (ethylene thiuram monosulphide), is a new fungicide with good prospects for agriculture. The mutagenic activity of technical endodan was evaluated with the m e t h o d of cytogenetic analysis of rat bone marrow after acute and short-term oral exposure. In the acute study groups of 6 male and female animals were treated with endodan at a dose of 1/5 LDs0 and then killed 6, 12, 24 and 36 h following administration. In the short-term studies several doses of endodan were administered to groups of 6 animals (a) twice at an interval of 24 h, (b) for 5 successive days. The animals were killed 6 h after the last administration. The cells in metaphase are analysed for aneuploidy and aberrations. Preliminary evaluation of the results failed to reveal mutagenic effects that could be attributed to the chemical. Horvat, Dj., R. Rozgaj, A. Bauman and J. Ra~id, Institute for Medical Research and Occupational Health, 41000 Zagreb (Yugoslavia)
Cytogenetic effect of uranyl nitrate and cadmium chloride on V79 Chinese hamster cells In technological processes where fossil fuels are used for energy production chemical and radiochemical contaminations are present. The most frequent pollutants are heavy metals and uranium compounds. The purpose of this work was to establish an in vitro model system in a mammalian cell culture. Two mutagenic agents, uranyl nitrate (UO~(NO3)2 × 6 H~O, 0.18 mg/ml and. 0.26 mg/ml) and cadmium chloride (CdC12, 10 -6 M and 10 -s M) were added to the nutrient medium. Chromosome aberrations were investigated after the agents were applied separately or simultaneously in different concentrations. In each experimental group of 300 analysed metaphases a significant increase of chromatid and chromosome damages was found. Djordjevid, O., and Lj. Kostid, Institute of Nuclear Sciences "Boris Kidri~", Radiobiological Laboratory, Beograd (Yugoslavia)
Potentiation of the effect of cytostatics in ~ m m R l i a n cells in vitro The present study was undertaken to investigate the effect of cytostatic drugs on the induction of chromosomal aberrations and the possibility of
Mutagenicity study of endodan in rats Endodan, etem (ethylene thiuram monosulphide), is a new fungicide with good prospects for agriculture. The mutagenic activity of technical endodan was evaluated with the m e t h o d of cytogenetic analysis of rat bone marrow after acute and short-term oral exposure. In the acute study groups of 6 male and female animals were treated with endodan at a dose of 1/5 LDs0 and then killed 6, 12, 24 and 36 h following administration. In the short-term studies several doses of endodan were administered to groups of 6 animals (a) twice at an interval of 24 h, (b) for 5 successive days. The animals were killed 6 h after the last administration. The cells in metaphase are analysed for aneuploidy and aberrations. Preliminary evaluation of the results failed to reveal mutagenic effects that could be attributed to the chemical. Horvat, Dj., R. Rozgaj, A. Bauman and J. Ra~id, Institute for Medical Research and Occupational Health, 41000 Zagreb (Yugoslavia)
Cytogenetic effect of uranyl nitrate and cadmium chloride on V79 Chinese hamster cells In technological processes where fossil fuels are used for energy production chemical and radiochemical contaminations are present. The most frequent pollutants are heavy metals and uranium compounds. The purpose of this work was to establish an in vitro model system in a mammalian cell culture. Two mutagenic agents, uranyl nitrate (UO~(NO3)2 × 6 H~O, 0.18 mg/ml and. 0.26 mg/ml) and cadmium chloride (CdC12, 10 -6 M and 10 -s M) were added to the nutrient medium. Chromosome aberrations were investigated after the agents were applied separately or simultaneously in different concentrations. In each experimental group of 300 analysed metaphases a significant increase of chromatid and chromosome damages was found. Djordjevid, O., and Lj. Kostid, Institute of Nuclear Sciences "Boris Kidri~", Radiobiological Laboratory, Beograd (Yugoslavia)
Potentiation of the effect of cytostatics in ~ m m R l i a n cells in vitro The present study was undertaken to investigate the effect of cytostatic drugs on the induction of chromosomal aberrations and the possibility of