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Cytogenetic studies of 35 meningiomas and their clinical significance
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Abstracts A 21 month old male presented with lower extremity paresis. A retroperitoneal tumor mass was found to involve the spinal canal, causing cord compression. Electron miero6copic analysis of the tumor and cells from passage 12 of the eel] line revealed diagnostic whorls of intermediate cytoplasmic filaments, dilated RER, cytoplasmic glycogen and cilia. No thick or thin filaments, microtubules or neuroseeretory granules were seen. Additionally, cells from the original tumor and the cell line stained positive for vimentin, EMA, S-100, neuron specific enolase and negative for desmin, let~kocyte common antigen, cytokeratin and alpha-feto protein. Cells from the patient's retroperitoneal tumor mass and from a pleural effusion were cultured using an extracellular matrix system end were harvested at peak mitotic activity. Twenty three of 30 cells from the retroparitoneal mass were normal,
, 818
CYTOGENETIC STUDIES OF 35 MENINGIOMAS AND THEIR CLINICAL SIGNIFICANCE.
Poulsg~rd, L. and Rsnne M. Department of Neurosurgery, Odense University Hospital and Institute of Anatomy and Cytolo~y, Odense University, Denmark. The histological appearance of meningiomas, which constitute about 15% of all primary brain tumors, is of little prognostic value. Meningiomas are considered to be a benign neoplasm, although malignant behavior is occasionally observed. Meningiomas represent tumors with a remarkably uniform chromosomal aberration. Partial or total monosomy 22 is present in most of the meningiomas with abnormal karyotype, although reports on frequencies 'of meningiomas with abnormal karyotype have varied considerably (from 56% to 96%). In this study cytogenetic analyses of 35 meningiomas are reported. Chromosomal abnormalities were found in all cases with monosomy 22 stemlines present in the majority. In some cases with additional sidelines the tumors were characterized by more a ~ r e s s i v e growth pattern than usually observed in meningiomas. In the additional sidelines hypodiploidy was m o s t often observed, and in a meningioma with frequent relapses we found a hyperhaploid stemline. Our observations thus seem to confirm that the general ~endency of clonal evolution in meningiomas is hypodiploidy, which may be correlated to a more aggressive behaviour of the tumor.
Abstracts A 21 month old male presented with lower extremity paresis. A retroperitoneal tumor mass was found to involve the spinal canal, causing cord compression. Electron miero6copic analysis of the tumor and cells from passage 12 of the eel] line revealed diagnostic whorls of intermediate cytoplasmic filaments, dilated RER, cytoplasmic glycogen and cilia. No thick or thin filaments, microtubules or neuroseeretory granules were seen. Additionally, cells from the original tumor and the cell line stained positive for vimentin, EMA, S-100, neuron specific enolase and negative for desmin, let~kocyte common antigen, cytokeratin and alpha-feto protein. Cells from the patient's retroperitoneal tumor mass and from a pleural effusion were cultured using an extracellular matrix system end were harvested at peak mitotic activity. Twenty three of 30 cells from the retroparitoneal mass were normal,
, 818
CYTOGENETIC STUDIES OF 35 MENINGIOMAS AND THEIR CLINICAL SIGNIFICANCE.
Poulsg~rd, L. and Rsnne M. Department of Neurosurgery, Odense University Hospital and Institute of Anatomy and Cytolo~y, Odense University, Denmark. The histological appearance of meningiomas, which constitute about 15% of all primary brain tumors, is of little prognostic value. Meningiomas are considered to be a benign neoplasm, although malignant behavior is occasionally observed. Meningiomas represent tumors with a remarkably uniform chromosomal aberration. Partial or total monosomy 22 is present in most of the meningiomas with abnormal karyotype, although reports on frequencies 'of meningiomas with abnormal karyotype have varied considerably (from 56% to 96%). In this study cytogenetic analyses of 35 meningiomas are reported. Chromosomal abnormalities were found in all cases with monosomy 22 stemlines present in the majority. In some cases with additional sidelines the tumors were characterized by more a ~ r e s s i v e growth pattern than usually observed in meningiomas. In the additional sidelines hypodiploidy was m o s t often observed, and in a meningioma with frequent relapses we found a hyperhaploid stemline. Our observations thus seem to confirm that the general ~endency of clonal evolution in meningiomas is hypodiploidy, which may be correlated to a more aggressive behaviour of the tumor.