- Email: [email protected]
Data on nine STR loci used for forensic and paternity testing in the Greek Cypriot population of Cyprus
Forensic Science International 123 (2001) 225±226
Announcement of population data
Data on nine STR loci used for forensic and paternity testing in the Greek Cypriot population of Cyprus Evy Bashiardesa, Panayiotis Manolia, Bruce Budowleb, Marios A. Carioloua,* a
DNA Identi®cation Laboratory, Cyprus Institute of Neurology and Genetics, Nicosia, Cyprus b Laboratory Division, FBI Academy, Quantico, VA, USA
Received 4 July 2000; received in revised form 16 October 2000; accepted 18 October 2000
Abstract Allele frequencies for the nine STRs included in the AMPF`STR kit were obtained from a sample of 152 unrelated Greek Cypriot from the Mediterranean island of Cyprus # 2001 Elsevier Science Ireland Ltd. All rights reserved. Keywords: Short tandem repeats; Population data; AMPF`STR pro®ler; Greek Cypriot; Cyprus
Population: Unrelated Greek Cypriot individuals. Extraction: Phenol±chloroform±isoamyl alcohol procedure [1]. PCR: The 1±2 ng target DNA following manufacturer's instructions [AMPF`STR pro®ler plus PCR ampli®cation kit (PE Applied Biosystems)]. Typing: ABI PRISM 310 Genetic Analyzer and reference sequenced ladders (PE Applied Biosystem). Results: See Table 1. Quality control: Pro®ciency testing through GEDNAP and ENFSI DNA WG.
Analysis of data: Statistical package provided by Ranjit Chakraborty (University of Texas School of Biomedical Sciences, Houston, TX, USA). Access to the data: Data available on CD-ROM or diskette upon request. Other remarks: A Greek Cypriot population database for nine loci has been developed to resolve forensic and paternity cases in Cyprus.
* Corresponding author. Tel.: 357-2-392654/392651; fax: 357-2-358237. E-mail address: [email protected] (M.A. Cariolou).
0379-0738/01/$ ± see front matter # 2001 Elsevier Science Ireland Ltd. All rights reserved. PII: S 0 3 7 9 - 0 7 3 8 ( 0 1 ) 0 0 5 3 6 - 9
226
E. Bashiardes et al. / Forensic Science International 123 (2001) 225±226
Table 1 Allele frequencies for AMPF`STR pro®ler plus loci in the Greek Cypriot population of Cyprusa Allele
D3S1358 N:152
VWA N:152
FGA N:152
D8S1179 N:152
D21S11 N:152
D18S51 N:152
D5S818 N:152
D13S317 N:152
D7S820 N:152
6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 21.2 22 22.2 23 23.2 24 25 26 26.2 27 27.2 28 28.2 29 30 30.2 31 31.2 32 32.2 33.2 H PD CE P
± ± ± ± ± ± ± ± 0.046 0.234 0.224 0.293 0.191 0.013 ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± 0.796 0.903 0.549 0.434
± ± ± ± ± ± ± ± 0.069 0.086 0.194 0.309 0.237 0.102 0.003 ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± 0.796 0.923 0.588 0.481
± ± ± ± ± ± ± ± ± ± ± ± 0.020 0.079 0.082 0.118 ± 0.204 0.003 0.161 ± 0.151 0.105 0.063 ± 0.007 ± ± ± 0.007 ± ± ± ± ± ± ± 0.908 0.962 0.732 0.678
± ± 0.026 0.010 0.076 0.089 0.102 0.243 0.207 0.181 0.043 0.023 ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± 0.809 0.952 0.679 0.468
± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± 0.007 ± ± 0.020 0.003 0.125 ± 0.220 0.220 0.036 0.063 0.111 0.010 0.135 0.049 0.842 0.957 0.698 0.938
± ± ± ± 0.016 0.016 0.168 0.158 0.181 0.138 0.102 0.085 0.059 0.029 0.036 0.009 ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± 0.875 0.966 0.740 0.609
± ± 0.013 0.052 0.138 0.224 0.345 0.224 0.003 ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± 0.809 0.888 0.536 0.083
± ± 0.128 0.036 0.063 0.312 0.302 0.134 0.023 ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± 0.724 0.915 0.562 0.638
0.003 0.013 0.161 0.095 0.276 0.257 0.168 0.026 ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± 0.763 0.924 0.594 0.195
a
H: observed heterozygosity; PD: power of discrimination; CE: a priori chance of exclusion; P: Hardy±Weinberg equilibrium; exact test based on 2000 shuf¯ings.
Reference [1] F.M. Auxbel, R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith, et al., Current Protocols in Molecular Biology, Wiley, New York, 1987.
Announcement of population data
Data on nine STR loci used for forensic and paternity testing in the Greek Cypriot population of Cyprus Evy Bashiardesa, Panayiotis Manolia, Bruce Budowleb, Marios A. Carioloua,* a
DNA Identi®cation Laboratory, Cyprus Institute of Neurology and Genetics, Nicosia, Cyprus b Laboratory Division, FBI Academy, Quantico, VA, USA
Received 4 July 2000; received in revised form 16 October 2000; accepted 18 October 2000
Abstract Allele frequencies for the nine STRs included in the AMPF`STR kit were obtained from a sample of 152 unrelated Greek Cypriot from the Mediterranean island of Cyprus # 2001 Elsevier Science Ireland Ltd. All rights reserved. Keywords: Short tandem repeats; Population data; AMPF`STR pro®ler; Greek Cypriot; Cyprus
Population: Unrelated Greek Cypriot individuals. Extraction: Phenol±chloroform±isoamyl alcohol procedure [1]. PCR: The 1±2 ng target DNA following manufacturer's instructions [AMPF`STR pro®ler plus PCR ampli®cation kit (PE Applied Biosystems)]. Typing: ABI PRISM 310 Genetic Analyzer and reference sequenced ladders (PE Applied Biosystem). Results: See Table 1. Quality control: Pro®ciency testing through GEDNAP and ENFSI DNA WG.
Analysis of data: Statistical package provided by Ranjit Chakraborty (University of Texas School of Biomedical Sciences, Houston, TX, USA). Access to the data: Data available on CD-ROM or diskette upon request. Other remarks: A Greek Cypriot population database for nine loci has been developed to resolve forensic and paternity cases in Cyprus.
* Corresponding author. Tel.: 357-2-392654/392651; fax: 357-2-358237. E-mail address: [email protected] (M.A. Cariolou).
0379-0738/01/$ ± see front matter # 2001 Elsevier Science Ireland Ltd. All rights reserved. PII: S 0 3 7 9 - 0 7 3 8 ( 0 1 ) 0 0 5 3 6 - 9
226
E. Bashiardes et al. / Forensic Science International 123 (2001) 225±226
Table 1 Allele frequencies for AMPF`STR pro®ler plus loci in the Greek Cypriot population of Cyprusa Allele
D3S1358 N:152
VWA N:152
FGA N:152
D8S1179 N:152
D21S11 N:152
D18S51 N:152
D5S818 N:152
D13S317 N:152
D7S820 N:152
6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 21.2 22 22.2 23 23.2 24 25 26 26.2 27 27.2 28 28.2 29 30 30.2 31 31.2 32 32.2 33.2 H PD CE P
± ± ± ± ± ± ± ± 0.046 0.234 0.224 0.293 0.191 0.013 ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± 0.796 0.903 0.549 0.434
± ± ± ± ± ± ± ± 0.069 0.086 0.194 0.309 0.237 0.102 0.003 ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± 0.796 0.923 0.588 0.481
± ± ± ± ± ± ± ± ± ± ± ± 0.020 0.079 0.082 0.118 ± 0.204 0.003 0.161 ± 0.151 0.105 0.063 ± 0.007 ± ± ± 0.007 ± ± ± ± ± ± ± 0.908 0.962 0.732 0.678
± ± 0.026 0.010 0.076 0.089 0.102 0.243 0.207 0.181 0.043 0.023 ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± 0.809 0.952 0.679 0.468
± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± 0.007 ± ± 0.020 0.003 0.125 ± 0.220 0.220 0.036 0.063 0.111 0.010 0.135 0.049 0.842 0.957 0.698 0.938
± ± ± ± 0.016 0.016 0.168 0.158 0.181 0.138 0.102 0.085 0.059 0.029 0.036 0.009 ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± 0.875 0.966 0.740 0.609
± ± 0.013 0.052 0.138 0.224 0.345 0.224 0.003 ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± 0.809 0.888 0.536 0.083
± ± 0.128 0.036 0.063 0.312 0.302 0.134 0.023 ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± 0.724 0.915 0.562 0.638
0.003 0.013 0.161 0.095 0.276 0.257 0.168 0.026 ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± 0.763 0.924 0.594 0.195
a
H: observed heterozygosity; PD: power of discrimination; CE: a priori chance of exclusion; P: Hardy±Weinberg equilibrium; exact test based on 2000 shuf¯ings.
Reference [1] F.M. Auxbel, R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith, et al., Current Protocols in Molecular Biology, Wiley, New York, 1987.