- Email: [email protected]
Dataset concerning haematological and biochemical parameters changes in show jumping horses subjected to exercise and plasmapheresis session
Journal Pre-proof Dataset concerning haematological and biochemical parameters changes in show jumping horses subjected to exercise and plasmapheresis session Réda Daden, Sara Chbihi, Fatima Zahraa Zarhouni, Jamal Chakir, Mohammed Piro, Mohamed Rachid Achaaban, Mohammed Ouassat, Khalid El Allali PII:
S2352-3409(19)31008-X
DOI:
https://doi.org/10.1016/j.dib.2019.104653
Reference:
DIB 104653
To appear in:
Data in Brief
Received Date: 22 July 2019 Revised Date:
31 August 2019
Accepted Date: 3 October 2019
Please cite this article as: R. Daden, S. Chbihi, F.Z. Zarhouni, J. Chakir, M. Piro, M.R. Achaaban, M. Ouassat, K. El Allali, Dataset concerning haematological and biochemical parameters changes in show jumping horses subjected to exercise and plasmapheresis session, Data in Brief, https://doi.org/10.1016/ j.dib.2019.104653. This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. © 2019 The Author(s). Published by Elsevier Inc.
1 2 3 4
Article Title Dataset concerning haematological and biochemical parameters changes in show jumping horses subjected to exercise and plasmapheresis session Authors
5
Réda Dadena,b, Sara Chbihia, Fatima Zahraa Zarhounia, Jamal Chakirc, Mohammed Pirod,
6
Mohamed Rachid Achaabana, Mohammed Ouassata& Khalid El Allalia.
7 8 9
Affiliations a
Comparative Anatomy Unit/URAC49, Department of Biological and Pharmaceutical
10
Veterinary Sciences, Hassan II Agronomy and Veterinary Medicine Institute, Rabat, Morocco.
11
b
Jumenterie de Zouada, Veterinary Hospital- Royal Guard, Tetouan-Morocco.
12
c
Veterinary Hospital- Royal Guard, Rabat-Morocco, Morocco.
13
d
14
Veterinary Medicine Institute, Rabat, Morocco.
15 16
PMC- EC, Department of Medicine, Surgery and Reproduction, Hassan II Agronomy and
Corresponding author
17
Khalid El Allali
18
Address: Hassan II Agronomy and Veterinary Medicine Institute,
19
B.P.6202 Rabat-Instituts, 1010, Rabat-Morocco.
20
Phone numbers: 00 212 6 61 14 04 70
21
Fax numbers: 00 212 0537 77 58 45
22
E-mail adresse : [email protected]
23
Abstract
24
This article presents data on the effect of plasmapheresis on clinical, haematological and
25
biochemical parameters in horses following exercise and after a plasmapheresis session. This
26
later was realized
27
(plasmapheresis group) that underwent three consecutive days of physical graded exercice
28
graded physical exercise. The control group (n=6) went through the same exercise but was not
This blood filtration technique was realised on six jumping horses
29
subjected to the plasmapheresis session. Blood was sampled before and after each exercise, and
30
also at the beginning and the end of plasmapheresis session. The presented data was obtained by
31
measuring clinical and haemato-biochemical parameters in both groups. The heart and
32
respiratory rates and rectal temperature were recorded. In addition, the number of red blood and
33
white cells, platelets and also of lymphocytes, monocytes, eosinophils and granulocytes were
34
counted. Other haematological parameters including, hemoglobin concentration, hematocrit,
35
mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin
36
concentration were determined. Concerning the biochemical parameters, the concentrations of
37
albumin, globulin, total protein, glucose, alkaline phosphatase, aspartate aminotransferase,
38
gamma glutamyl transferase, total bilirubin, lactate, creatinine kinase, urea, creatinine, calcium,
39
sodium and potassium were measured. All parameters data were analyzed by a two-way repeated
40
measures analysis of variance followed by Holm-Sidak post-hoc procedure to evaluate the effect
41
of plasmapheresis and time. This paper contains data related to and supporting research articles
42
currently published entitled "Plasmapheresis effect on haematological and biochemical
43
parameters in athletic horses subjected to exercise" (Daden et al, 2019) [1].
44 45
Keywords
46 47 48 49 50
Plasmapheresis, horse, exercise, haemato-biochemical parameters.
Specifications Table Subject
Veterinary Science and Veterinary Medicine
Specific subject area
Equine medicine, Equine exercise, Exercise physiology, Blood filtration technique, Plasmapheresis, Haematology Biochemistry
Type of data
Tables, Figure
How data were acquired
-Physiological parameters: Physiological parameters were measured in the two groups using the same procedures as in clinical examination. • Heart rate: the pulse on the facial artery was taken. • Respiratory rate: Horse's chest movements were counted.
•
Rectal temperature: recorded using a digital thermometer (range, 32– 42°C; accuracy, 0.1°C). -Plasmapheresis: was performed by using a Hemofenix® plasmapheresis machine commercialized by Hemofenix-France utilizing Trackpore® technology (Dubna Moscow region-Russian Federation). -Blood sampling: Blood samples were obtained by jugular venipuncture and were collected in BD-Vacutainer EDTA tubes for haematological parameters and in lithium heparin’BD-Vacutainer tubes for biochemical parameters. -Haematological parameters analysis was performed by using a veterinary cell-counter automate, the Celltac VET MEK-6550® haematology (Nihon Kohden, Tomioka-Japan) and its reagents, Hemolynac·3 MEK-660I, Isotonac·4 MEK-641I, Cleanac MEK520I (Celltac VET MEK-6550 J/K haematology, Nihon Kohden, Tomioka-Japan). -Biochemical parameters were assayed using the veterinary SkylaVB1® automate analyzer (Lite-On Technology Corporation, Hsinchu-Taiwan) and reagent disc (Equine Panel- Product code 900150, skylaTM VB1® reagent disc, Hsinchu-Taiwan). -Lactate concentrations were determined using Lactate-Pro™ 2 analyzer (Busimedic®, S.L, San Sebastián-Spain). Data format
Raw Data
Parameters for data collection
The data were obtained from 12 show jumping horses that were divided into plasmapheresis (n=6) and control (n=6) groups. Each group was formed by contained 3 females and 3 males of AngloHispano-Arab breed, aged from 4 to 12 years and weighing 400kg. Throughout the study, horses were healthy and their clinical state was rigorously controlled.
Description of data collection
All horses underwent the same daily physical exercise for three consecutive days. Following the 3rd day exercise, a plasmapheresis session was performed only on plasmapheresis group horses horses of plasmapheresis group. All physiological and haematobiochemical parameters data presented in this article were obtained from blood samples taken simultaneously on horses of the plasmapheresis and control groups before and after each exercise and after plasmapheresis session for the following 4 days.
Data source location
Institution: Royal Guard of Tétouan City/Town/Region: Tétouan Country: Morocco Latitude: 35.5730287 Longitude : -5.3515084 GPS Coordinates: 35° 34' 22.904" N 5° 21' 5.429" W
Data accessibility
Raw Data is available with this article
Related research article
Author’s name: Réda Dadena,b, Fatima Zahraa Zarhounia, Jamal Chakirc, Mohammed Pirod, Mohamed Rachid Achaabana, Mohammed Ouassata & Khalid El Allalia. Title: “Plasmapheresis Effect on Haematological and Biochemical Parameters in Athletic Horses Subjected To Exercise” Journal: Journal of Equine Veterinary Science DOI: https://doi.org/10.1016/j.jevs.2019.07.006
51 52 53
Value of the Data
54 55 56 57 58 59 60 61 62 63 64 65
•
These data highlight the effect of plasmapheresis and demonstrated that this blood filtration technique is able to modify the physiology post-exercise of the horse and to affect both the haematology and the biochemistry of the blood.
•
This data represents for the first time the changes in physiological, haematological and biochemical parameters in horses subjected to a plasmapheresis session after three days of exercise. It can be thus taken into consideration by other researchers interested in both sport physiology and blood filtration techniques field.
•
Such data can be used as a reference series for comparative approaches in the field. By providing relevant information, it allows a reuse and a better interpretation of the results. This is also useful for future studies.
66 67
•
The data demonstrate for the first in horse that plasmapheresis could be considered a s a doping technique.
•
In addition, the present data show informations that are complementing the related article and can be interesting for readers of the Data in Brief journal.
68 69 70 71
72 73 74
Data shown in this article provide information about the effect of plasmapheresis on
75
physiological, haematological and biochemical parameters in athletic horses subjected to
76
exercise. Horses of both plasmapheresis and control groups underwent each morning an exercise
77
of 30 min/day, during 3 successive days (Day -2, -1 and 0). Following the exercise session of the
78
3rd day (Day 0) a plasmapheresis session was performed only on the horses of the plasmapheresis
79
group. The control group were maintained in the same environment but without being subjected
80
to plasmapheresis. Data of haemato-biochemical parameters were obtained by analyzing blood
81
samples collected before and after exercise and plasmapheresis. Tables 1, 2 and 3 describe
82
clinical parameters variations subsequent to an exercise and a plasmapheresis session. Tables 4
83
to 10 represent each one parameters of red hemogram before and after exercise also before and
84
after a plasmapheresis session. Tables 11 to 15 describe parameters of white hemogram and their
85
changes subsequent to exercise and plasmapheresis session. Tables 16 to 30, represent variations
86
of biochemical parameters before and after graded exercise and plasmapheresis session.
87
Figure 1 illustrate Lactate concentrations before and following the exercise test standardized by
88
Demonceau and Auvinet [2], also before and following the modification of the warm-up phase of
89
the same exercise test.
90 91 92
1. Data
1.1.Tables
93
The following tables containing raw data concerning changes in physiological, heamtological
94
and biochemical parameters of horses subjected to subsequent to graded physical exercise and
95
plasmapheresis session. Data of each sample are presented individually for animals of the control
96
and plasmapheresis groups.
97
measurements after the exercise; S7: immediate measurement after the plasmapheresis session;
98
S8, S9, S10, S11: post-plasmapheresis measurements at rest. D-2: 48h before plasmapheresis D-
99
1: 24h before plasmapheresis; Day 0: day of plasmapheresis subsequent to the last exercise; D1,
100 101 102 103 104
S1, S3, S5: measurements before the exercise; S2, S4, S6:
D2, D3 and D4 coresspond to 24h, 48h, 72h and 96h respectively after plasmapheresis.
105 106 107
Table 1 Rectal temperature (°C)* variations in jumping horses subsequent to an exercise and a plasmapheresis session.
Horse
D-2 S1 S2
Time (Days / Samples) D-1 D0 D1 D2 D3 D4 S3 S4 S5 S6 S7 S8 S9 S10 S11
Control Group
ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO
37.5 37.2 37.8 37.6 37.5 37.8
40.1 40 40.6 39.9 39.9 41
37.5 37.8 37.6 37.6 37.6 37.6
40.2 40.8 40.6 39.9 40 39.9
37.5 37.5 37.5 37.6 37.6 37.6
40.3 40.9 40.5 40.1 40.1 40.1
38.1 37.5 38.1 38.2 38.3 38.2
37.5 38.3 37.9 37.5 37.6 37.5
37.8 38.6 37.6 37.5 37.5 37.5
37.6 38.5 37.5 37.5 37.5 37.5
37.5 38.6 37.6 37.5 37.6 37.5
Plasmapheresis Group
BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
37.6 37.5 37.7 37.7 37.6 37.8
39.9 40.8 40.9 40.6 40.6 39.9
37.6 37.5 37.8 37.8 37.6 37.9
39.9 40.9 40.8 41 40.6 40.2
37.6 37.5 37.7 37.8 37.5 37.5
40.1 40.9 40.9 40.9 40.5 40.1
38.2 37.5 37.9 40.1 38.1 39.1
37.5 38.3 37.8 37.9 37.9 38.2
37.5 38.6 37.8 37.8 37.6 37.5
37.5 38.5 37.9 37.8 37.5 38.5
37.5 38.6 37.8 37.8 37.6 39.5
Group
108 109 110 111 112
*Normal rectal temperature of adult horse : 37.0 – 38.0 °C [3]. Table 2 Heart rate (beats per minute)* changes in jumping horses subsequent to an exercise and a plasmapheresis session.
Group
113 114 115 116 117
Horse
Control Group
ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO
Plasmapheresis Group
BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
Time (Days / Samples) D-1 D0 D1 D2
D-2 S1
S2
S3
S4
S5
S6
S7
S8
S9
36 36 32 36 36 36 36 36 45 41 32 38
112 120 120 110 120 115 110 104 167 110 110 112
36 36 36 36 36 36 36 36 44 44 36 40
116 126 132 115 132 110 113 100 162 120 120 132
36 36 36 36 36 36 36 36 44 44 36 42
120 123 123 110 152 110 100 120 152 118 114 110
38 36 38 36 36 36 36 36 46 45 38 40
36 36 36 36 36 36 36 36 44 44 36 36
36 36 36 38 38 38 38 36 45 42 36 38
D3
D4
S10 S11 38 36 38 36 36 36 36 36 46 44 38 38
36 36 36 36 38 36 36 36 44 40 36 36
*Normal heart rate of adult horse: 30 – 40 beats per minute [3]. Table 3 Respiratory rate (breaths per minute)* changes in jumping horses subsequent to an exercise and a plasmapheresis session. Time (Days / Samples)
Group
Control Group
Plasmapheresis Group 118 119 120 121 122 123 124
D-2 S1 S2
D-1 S3 S4
S5
D0 S6
S7
D1 D2 D3 D4 S8 S9 S10 S11
ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
24 20 20 20 20 20 20 24 28 28 24 24
26 24 24 28 28 28 28 20 32 28 24 28
26 20 20 24 24 24 24 24 28 26 20 24
68 72 66 66 66 68 66 68 72 68 68 66
28 24 24 28 28 28 28 24 24 28 24 28
24 28 24 24 24 24 24 28 28 26 24 24
68 68 68 64 64 66 64 64 98 64 68 68
68 64 62 68 68 68 68 72 68 63 63 68
24 24 20 22 22 22 22 24 28 24 20 28
24 28 24 24 24 24 24 28 28 24 24 26
24 24 24 24 24 24 24 24 28 24 24 24
*Normal respiratory rate of adult horse: 8 – 20 breaths per minute [4].
Table 4 Red blood cell count (106/µl)* changes in jumping horses subsequent to exercise and a plasmapheresis session.
Horse
D-2 S1 S2
Time (Days / Samples) D-1 D0 D1 D2 D3 D4 S3 S4 S5 S6 S7 S8 S9 S10 S11
Control Group
ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO
8.88 8.63 8.71 7.59 7.59 7.53
10.6 13.3 12.2 12.1 12.1 12.1
8.58 8.58 9.42 9.74 9.74 9.74
10.4 10.4 11.9 11.6 11.6 11.6
10.2 8.61 11.2 9.34 8.02 8.1
13.2 10.7 14.3 11.2 11.9 12.4
8.01 8.67 10 10.2 7.99 9.52
7.47 10.3 8.49 7.58 7.58 7.75
7.92 9.6 9.11 7.75 7.75 7.53
8.62 8.17 8.56 7.53 7.53 7.74
8.71 8.63 9.01 7.56 7.56 13.4
Plasmapheresis Group
BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
11.7 11.2 8.26 7.56 8.71 7.92
11.3 15.3 11.4 12.1 12.2 11.4
9.74 9.94 8.3 9.42 9.42 7.39
12.9 14.5 11.5 11.9 12.3 11.5
10.2 11.2 8.35 9.79 10.4 8.35
13.1 14.7 11.3 12.6 12.4 11.3
13.2 13.5 11.9 12.8 12.7 13
12.3 12.1 10.3 10.2 8.03 7.25
10.6 10.3 10.2 7.96 6.95 8
10.4 9.27 7.96 7.82 7.22 8.19
9.31 10.3 7.43 10.2 10.5 8.39
Group
125 126 127 128 129
Horse
*Reference range: 6.70 – 12.90.106 /µL [5]. Table 5 Changes in blood hemoglobinconcentrations (mg/dL) (g/dl)* in jumping horses subsequent to exercise and a plasmapheresis session.
Group
Horse
D-2 S1 S2
Time (Days / Samples) D-1 D0 D1 D2 D3 S3 S4 S5 S6 S7 S8 S9 S10
D4 S11
130 131 132 133 134 135 136 137
Control Group
ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO
14.2 13.3 13 11 11 11.1
16.9 19.3 17.3 18.2 15.6 18.2
13.7 13.2 14.1 15.5 11.5 15.5
17.5 16.7 17.4 17.6 14.3 17.6
11.7 13.2 14.5 15.8 11.6 15.8
17.4 16.8 17.8 16.2 14.1 18.1
13 13.5 12.9 12.1 11.3 11.8
12.3 15.8 12.9 11.3 12.7 10.9
13.2 14.6 13.4 10.9 11.3 11
14.1 12.7 13.2 11 11.9 11
14.3 13.4 13 10.8 12.7 18.9
Plasmapheresis Group
BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
17 15.2 12.2 10.5 13 10.8
17.7 24.4 17.3 18 17.3 17.3
15.5 13.4 12.1 14.1 14.1 10.3
19.4 19.2 17 17.4 17.4 17
15.3 14.3 12 14.5 16.8 12
19.8 19.7 16.8 17.1 17.5 16.8
19.6 19.2 17 19.2 17.5 17
14.6 13.9 12 12.8 12.2 12.3
14.8 13.5 12.2 12 12.3 11.9
15.6 12.5 11.8 10.7 10.8 12.6
13.8 14.5 11.1 13.6 15.8 12.2
*Reference range: 11.0 – 19.0 g/dl [6].
Table 6 Blood hematocrit (g/dL)* variations changes in jumping horses subsequent to exercise and a plasmapheresis session.
Group
Control Group
Plasmapheresis Group 138 139 140 141 142 143
Horse
D-2 S1 S2
Time (Days / Samples) D-1 D0 D1 D2 D3 D4 S3 S4 S5 S6 S7 S8 S9 S10 S11
ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
43.9 40.4 39.5 34 36.6 33 37.5 37.9 36.1 38.5 42.1 37
42.5 40.4 42.5 43.8 36.8 42.9 43.8 41.3 36.8 42.5 42.5 32.8
52.5 59.8 52.4 54.9 48.5 54.9 53.3 53 51.3 55.2 52.4 51.3
51.7 54 52.4 50 53 52 57.9 53 53 52.4 52.4 51.4
36.5 40.3 44.4 43 35.9 43 45.7 43 37 44.4 45 33.8
52.9 50.8 53.3 49 56.1 54.8 59.2 61.4 51.2 53.9 53 51.2
39.5 41.1 38.9 36.2 48.9 36 59 60.9 53 54 50.6 53
39.9 44.9 38.5 38.5 43.1 37.7 31.9 32.8 32.7 31.2 37.7 34.7
38 39 36 37.4 49 36 35.4 33.3 31.1 40.1 36.8 36.7
35.2 37.7 34.2 34.9 51.3 37 33.7 32.6 28.2 37.3 38.1 33
34 39.5 38.8 35.6 34.4 34.2 35.6 32.8 30.6 31.3 35.4 33.8
*Reference range: 32.0 – 53.0 g/dl [6].
Table 7 Changes in blood mean corpuscular volume (fL)* in jumping horses subsequent to exercise and a plasmapheresis session.
Group
Horse
Control Group
ACOLITO
D-2 S1 S2 44.8
46
Time (Days / Samples) D-1 D0 D1 D2 D3 S3 S4 S5 S6 S7 S8 S9 S10 45
46
44.6
45.2 44.8 44.6
44.4
44.5
D4 S11 44.6
Plasmapheresis Group 144 145 146 147 148
41.4 44.3 42.1 45 43.4 45.4 46.8 41.4 44.8 41.2 44.8
44 45.2 45.6 46.8 45.2 46 47 43 45.4 42.8 45.4
41.5 44.2 45.4 44.6 43 45.5 46.3 41.3 45 40.9 45
44.1 45 45.5 45.5 45.3 46.1 47.4 45.5 45.6 42.3 45.6
41.3 44.3 45.4 44.7 44.3 44 45 45.4 45.2 41.2 43
43 45.2 43 45.2 45.2 45.5 46 45.9 45.5 42.1 43.1
41.7 44.9 42.7 45.6 43.9 45.5 47.1 45.2 45.3 42.4 43.2
41.3 44.4 42.3 45.3 43 44 45 45.1 45.1 41.6 44.9
41.6 44.1 42.2 45 43.6 44.9 44 44.9 45 41.3 45
41.2 44.3 42.2 45.3 45.5 45 47.2 45.2 45 41.8 43
40.9 44.5 42.2 45.5 43.9 45 47 44.9 44.7 41.7 42.9
*Reference range: 37.0 – 59.0 fL [6]. Table 8 Changes in blood mean corpuscular hemoglobin (pg)* in jumping horses subsequent to exercise and a plasmapheresis session.
Group
Control Group
Plasmapheresis Group 149 150 151 152 153
DAHBI CHAMAA DAHMAN DAMAA DALILANO BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
Horse ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
D-2 S1 S2
Time (Days / Samples) D-1 D0 D1 S3 S4 S5 S6 S7 S8
D2 S9
D3 S10
D4 S11
14.9 13.5 14.8 14.5 14.9 14 15 15.6 14 14.5 13.9 14.5
14.9 13.8 14.7 14.7 14.7 13.8 15.2 15.4 14 14.6 13.5 14.3
14.8 13.6 15 13.9 15.1 14.1 14.9 15.6 14.3 14.5 13.6 14.6
14.8 13.5 15.1 13.7 15 14.6 15.2 15.5 14.9 14.6 13.6 14.2
14.8 13.2 14.9 13.4 15.1 15.1 15 15.5 14.5 14.8 13.8 14.1
15.7 15.4 15.2 14.9 15 15.2 16 16.2 14.1 15 14.1 15
15.1 15.5 15 15.1 15.1 15 16.2 16.1 14.6 14.9 14.2 15
14.9 13.1 14.4 14.8 14.7 14.4 15 15.5 14 14.4 13.9 14.5
15.1 15.2 14.8 15.3 15.4 15.2 16.3 15.7 15.3 14.7 13.8 14.6
14.8 14.5 14.4 14.2 15.3 14.7 16.2 15.8 15.4 14.9 13.5 14.8
14.5 13.8 14.9 14 15.2 14.6 15 15.4 14.3 14.6 13.9 14.5
*Reference range: 12.3 – 19.9 pg [7]. Table 9 Changes in blood mean corpuscular hemoglobin concentration (g/dL)* in jumping horses subsequent to exercise and a plasmapheresis session.
Group
Control Group
Plasmapheresis
Horse ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO BELUC CHADDY
D-2 S1 S2 32.3 32.9 32.9 32.4 32.9 32.4 32.4 32.7
33 33.3 33 33.2 33 33.2 33.2 33.2
Time (Days / Samples) D-1 D0 D1 S3 S4 S5 S6 S7 S8 32.2 32.7 32.8 32.3 32.8 32.3 32.5 33.2
33.8 33.9 33.1 33.1 33.1 33.1 33.5 33.2
32.1 32.8 32.7 32.4 32.7 32.4 32.5 32.6
32.9 33.1 33.4 33.1 33.4 33 33.4 33.1
32.9 32.8 33.1 33.4 33.2 33 33.1 33.6
33.4 32.8 32.5 32.7 32.5 32.9 32.4 33.6
D2 S9 33.1 33.2 32.8 32.5 32.1 33 32.3 33
D3 D4 S10 S11 32.8 33.1 32.8 32.6 32 33 32 32.7
32.5 33 32.9 32.6 33 32.6 32.5 32.1
Group
154 155 156 157 158 159
33.3 33.7 32.8 33 32.6 32.8 32.9 33 32.8 32.3 33.7 32.1
33.5 33.1 33.1 33.5
32.4 32.7 32.2 32.4
33.2 33.1 33.2 33.6
32.2 32.1 32 31.6 32.1 32 32.4 32.6 32.5 33.2 32 32.9
32.5 32.4 32.6 33
33.1 32.6 32.5 33.2
*Reference range: 31 – 38.6 g/dl [7].
Table 10 Blood platelet count (103/µl)* variations in jumping horses subsequent to exercise and a plasmapheresis session.
Horse
D-2 S1 S2
Time (Days / Samples) D-1 D0 D1 S3 S4 S5 S6 S7 S8
Control Group
ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO
172 152 125 143 152 143
179 146 170 127 170 127
171 146 136 143 149 143
193 165 198 119 198 119
155 148 134 141 134 141
173 145 164 119 180 114
164 149 133 131 128 95
143 135 147 150 139 152
158 139 158 152 147 148
152 144 149 148 156 133
148 135 153 151 163 77
Plasmapheresis Group
BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
144 154 159 145 125 157
162 154 173 151 170 173
143 153 161 136 136 179
134 157 173 198 198 173
144 156 145 134 127 145
122 140 171 157 186 171
138 156 150 151 11 174
141 150 144 151 109 157
159 150 148 142 111 168
170 104 155 143 114 194
136 150 157 110 73 135
Group
160 161 162 163 164 165
PRINTANIER DAOUDIYA CAMELIA DOUALIYA
D2 D3 D4 S9 S10 S11
*Reference range: 100 – 270 103/µL [5].
Table 11 White blood cell count (103/µl)* changes in jumping horses subsequent to exercise and a plasmapheresis session.
Group
Control Group
Plasmapheresis Group
Horse
D-2 S1 S2
Time (Days / Samples) D-1 D0 D1 S3 S4 S5 S6 S7 S8
ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
8.7 5.6 10.8 8 7.9 8.1 7.8 11.1 9.3 8.8 10.8 9.4
8.4 6.3 10.6 8.4 8.6 8.4 8.4 10.6 9.4 10.6 10.6 9.7
10.4 8.1 14.2 10.1 10.6 10.1 7.5 15.3 11.7 13.8 14.2 11.7
9.9 7.7 13.9 12.1 12.6 12.1 7.7 13.4 11.6 13.9 13.9 11.6
9.1 8.2 10.2 8.5 10.2 10 9 11 9.9 10.7 10.4 9.9
9.5 10 13.2 11 10.5 8.9 8.1 16.4 11.8 12.3 14.3 11.8
D2 D3 D4 S9 S10 S11
11.8 8.5 7.9 8.1 8 7.6 5.8 5.7 5.6 5.1 14.5 11.1 10.8 10.7 11 12.4 9.3 8.9 8.6 7.2 7.6 8.4 7.6 7.8 7.9 9.9 8.9 8.6 10.2 5.9 12.6 8.7 7.8 7.6 7.6 16.8 14.5 11.3 10.1 11.3 12 9.4 8.8 8.2 7.8 12.5 10.3 9.8 8.1 5.8 14.6 9 8.6 7.8 6.9 12.4 9.3 9.7 9.3 9
166 167 168 169 170
*Reference range: 5.4 -14.3 103/µL [5]. Table 12 Changes in blood lymphocyte count (103/µl)* in jumping horses subsequent to exercise and a plasmapheresis session.
Group
Control Group
Plasmapheresis Group 171 172 173 174 175
D-2 S1 S2
ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
6.9 5.2 6.9 5.9 5.3 5.8 4 12.5 5.9 6.9 6.4 5.9
6.3 5.6 6.3 5.8 5.4 6.3 6.6 5.9 5.8 6.4 6.4 5.8
8.2 10.6 10.9 10.6 9.3 9.5 7.1 9.9 8.8 9.7 13.1 9.2
8.9 10.3 10.3 11.5 10.3 6.3 7.9 9.7 9 9.6 9.1 10
7.9 6.4 6.8 5.9 7.4 6.2 8.3 6.3 7.3 7.3 7.2 7.9
9.6 9.1 7.2 9.6 9.2 9.4 8.6 6 10.6 9.6 10.6 12
7.3 8.3 10.1 9.8 8.6 9.3 8.8 7.2 10.8 10.2 10.9 12.6
7.2 6.2 6.2 7.5 6.5 7.5 7.5 7.9 8.5 8.1 7.2 7
D2 D3 D4 S9 S10 S11 7 5.5 7.5 7.2 6.3 6.2 6.2 7.8 8.1 7.5 6.9 6.9
6.5 5.6 6.4 5.8 5.9 5.5 5.7 6 7.8 7.2 6.8 6.8
6.4 5.7 6.2 5.9 5.5 6.2 5.6 6.5 6 6.8 6.3 5.8
*Reference range: 1.5 – 7.7 103/µl [6]. Table 13 Variations of blood Monocyte count (103/µl)* in jumping horses subsequent to exercise and a plasmapheresis session.
Group
Control Group
Plasmapheresis Group 176 177 178 179 180
Horse
Time (Days / Samples) D-1 D0 D1 S3 S4 S5 S6 S7 S8
Horse
D-2 S1 S2
Time (Days / Samples) D-1 D0 D1 S3 S4 S5 S6 S7 S8
ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
0.2 0.3 0.3 0.2 0.3 0.2 0.2 0.1 0.3 0.2 0.3 0.2
0.2 0.2 0.3 0.3 0.3 0.3 0.1 0.1 0.2 0.2 0.4 0.2
0.3 0.3 0.3 0.4 0.3 0.4 0.3 0.3 0.2 0.4 0.3 0.2
0.2 0.3 0.4 0.5 0.4 0.5 0.3 0.3 0.2 0.3 0.3 0.4
0.2 0.2 0.3 0.2 0.3 0.2 0.2 0.2 0.3 0.3 0.2 0.2
0.3 0.3 0.5 0.4 0.4 0.5 0.4 0.6 0.3 0.3 0.4 0.3
0.4 0.2 0.3 0.2 0.3 0.2 0.4 0.5 0.2 0.4 0.6 0.2
0.2 0.2 0.2 0.3 0.2 0.3 0.2 0.4 0.6 0.2 0.1 0.2
D2 D3 D4 S9 S10 S11 0.2 0.1 0.2 0.3 0.2 0.3 0.3 0.3 0.2 0.3 0.2 0.2
0.3 0.3 0.1 0.1 0.2 0.3 0.3 0.1 0.3 0.3 0.2 0.2
*Reference range: 0 - 0.8 103/µl [8]. Table 14 Blood Eosinophil count (103/µl)* changes in jumping horses subsequent to exercise and a plasmapheresis session.
0.4 0.1 0.2 0.2 0.3 0.1 0.4 0.4 0.1 0.1 0.2 0.2
Group
Control Group
Plasmapheresis Group 181 182 183 184 185
D-2 S1 S2
ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
0.2 0.3 0.3 0.3 0.3 0.2 0.3 0.1 0.4 0.4 0.3 0.2
0.1 0.4 0.3 0.1 0.3 0.2 0.3 0.2 0.3 0.3 0.3 0.4
0.2 0.2 1.3 0.6 1.3 0.6 0.4 0.9 0.8 1.4 1.3 0.9
0.2 0.4 0.9 0.4 0.9 0.4 0.1 0.7 0.9 0.9 0.9 0.9
0.1 0.3 0.3 0.3 0.3 0.3 0.3 0.2 0.3 0.3 0.4 0.5
0.6 0.9 0.7 0.9 0.8 0.9 0.6 1.6 0.8 0.8 1 0.8
0.4 0.5 0.3 0.5 0.5 0.6 0.1 1.5 0.9 0.9 0.8 0.9
0.2 0.1 0.3 0.3 0.4 0.2 0.3 0.5 0.4 0.4 0.3 0.4
D2 D3 D4 S9 S10 S11 0.2 0.2 0.3 0.3 0.4 0.2 0.1 0.4 0.1 0.6 0.7 0.4
0.1 0.3 0.2 0.2 0.3 0.2 0 0.3 0.3 0.5 0.2 0.9
0.1 0.3 0.1 0.3 0.2 0.1 0 0.2 0.5 0.3 0.3 0.2
*Reference range: 0 – 1.0 103/µl [6]. Table 15 Blood Granulocyte count (103/µl)* variations in jumping horses subsequent to exercise and a plasmapheresis session.
Horse
D-2 S1 S2
Time (Days / Samples) D-1 D0 D1 S3 S4 S5 S6 S7 S8
Control Group
ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO
5.9 3.2 6.1 3.7 7.9 3.7
6.1 5.3 6.2 3.6 10.6 3.6
5.1 3 3.5 3.5 8.6 6.1
9.6 5.8 8.6 8.5 7.6 3.3
5.4 1.9 6.3 5.3 7.6 5.3
5.7 2.6 6.4 6.8 12.6 6.8
6.1 3 6.4 5.2 8.4 4.9
5.4 2.6 6.4 4.8 7.8 3.8
5.5 2.9 6.2 4.9 6.5 4.5
5 0.5 5.7 3.6 10.5 2.1
5.3 2.6 6.3 3.4 7.9 2
Plasmapheresis Group
BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
2.8 2.8 4.6 5.5 6.2 4.6
5.2 7.4 5.2 6.3 7.5 6.5
5.2 2.5 5.7 5.1 6.5 5.7
6.3 6.5 6.3 6.4 7.2 6.8
6.3 3.4 5.1 6.3 3.4 5.1
6.1 7 6.5 6.5 7.3 6.2
6.5 7.3 6.3 6.5 7.5 6.4
6.3 3.5 5.1 6.6 4.7 5
4.6 3.4 4.8 5.4 4.3 5.1
4.3 3.5 4.2 4.3 4 5
5.1 3.2 4.1 2.8 3.2 4.6
Group
186 187 188 189 190 191
Horse
Time (Days / Samples) D-1 D0 D1 S3 S4 S5 S6 S7 S8
D2 D3 D4 S9 S10 S11
*Reference range: 2.3 – 8.6 103/µl [7]. Table 16 Variations of glucose (mmol/L) concentrations subsequent to exercise and a plasmapheresis session. Blood glucose concentration (mmol/L)* in jumping horses submitted to plasmapheresis after graded exercise.
Group Control Group
Horse
D-2 S1 S2
Time (Days / Samples) D-1 D0 D1 S3 S4 S5 S6 S7 S8
ACOLITO
5.2
4.5
6
6
4.5
5.6
5.7
5.7
D2 D3 D4 S9 S10 S11 5.6
5.5
5.6
Plasmapheresis Group 192 193 194 195 196 197
7.2 5.5 4.6 5.5 4.6
6 7.1 5.6 7.1 5.6
4.9 6.2 4.5 6.2 4.5
5.8 5.8 5.7 8.2 5.7
4.8 5.3 5.3 6.6 5.3
6.8 6.9 6.3 6.2 6.3
5.3 6.2 5.7 5.8 5.1
5.7 5.8 5.4 5.6 4.8
5.4 5.3 5.3 5.3 5.3
5.3 5.6 4.9 5.6 4.9
5.2 5.9 4.8 6.2 5.4
BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
4.8 5.6 3.8 5.5 7.8 5.8
5.6 5.8 6.5 7.1 5.3 7.1
5.3 4.9 3.6 6 5.5 5.9
6.3 6.6 4.5 6.3 5.6 8.2
5.4 4.9 4.5 6.1 5.4 5.6
5.2 9.3 6.4 6.3 5.4 5.8
5.4 5.5 5.7 5.5 3.6 5.9
5.2 5.5 4.8 6.8 4.8 5
5.1 5.4 4.7 6.7 4.7 4.7
4.9 4.9 4.6 5.9 4.8 4.6
4.6 4.6 4.6 5.6 4.7 5.5
*Reference range: 3.5 – 6.0 mmol/L [9].
Table 17 Modifications of blood urea concentrations (mmol/L)* in jumping horses subsequent to exercise and a plasmapheresis session.
Horse
D-2 S1 S2
Time (Days / Samples) D-1 D0 D1 S3 S4 S5 S6 S7 S8
Control Group
ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO
4.1 4.8 5.3 5 3.9 5
4.2 5.3 5.9 5.4 4.1 5.4
4.1 4.9 5.6 5.2 4 5.1
4.1 5.4 6.3 5.8 4.2 5.8
3.8 5.1 6 5.3 4.5 5
3.9 5.5 6.2 5.6 5 5.7
3.9 5.5 5.8 5.3 4 4.9
3.8 5.6 5.6 5.4 3.9 4.9
3.9 5.1 5.3 5.6 3.8 4.8
3.8 4.9 4.6 4.9 3.7 4.7
3.6 4.7 4.7 4.6 3.5 4.6
Plasmapheresis Group
BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
4.3 3.9 4.5 3.8 6 5.1
4.9 3.9 4.6 4.1 6.5 5.2
4.6 4 4.8 3.9 6 5.1
4.7 4.3 5 4.2 6.5 5.2
4 3.8 5.1 4.6 6.3 4.9
4.2 3.9 5.8 5.3 7.8 4.6
3.6 3.2 4.9 5 6.3 4.3
3.2 3.5 4 5.1 5.6 4.2
3.8 3.2 4.1 5.3 5.4 4
3.7 3 4.1 4.2 5.6 4.1
3.2 3.1 4 3.1 5.6 4
Group
198 199 200 201 202
DAHBI CHAMAA DAHMAN DAMAA DALILANO
D2 D3 D4 S9 S10 S11
*Reference range: 1.2 à 3.6 mmol/L [9]. Table 18 Changes in blood creatinine concentrations (mg/dL)* in jumping horses subsequent to exercise and a plasmapheresis session.
Group
Control Group
Horse
D-2 S1 S2
Time (Days / Samples) D-1 D0 D1 S3 S4 S5 S6 S7 S8
ACOLITO DAHBI CHAMAA DAHMAN DAMAA
1.9 1.7 1.2 1.8 1.4
1.9 1.8 1.2 1.9 1.4
2.2 1.9 1.7 2.5 1.7
2 2.1 1.9 2.4 1.9
2 2 1.3 1.8 1.5
2.4 2.3 1.8 1.9 2
2 2.1 1.3 2 2.2
1.8 2 1.8 1.6 1.8
D2 D3 D4 S9 S10 S11 1.9 2.3 1.7 1.7 1.7
2 2.1 1.5 1.8 1.5
2.1 2 1.4 1.6 1.6
Plasmapheresis Group 203 204 205 206 207
1.8
2.5
1.9
2.4
2.1
2.2
2.1
1.8
1.7
1.8
1.5
BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
1.6 1.5 1.7 1.3 1.4 1.6
2.3 1.5 2.3 1.7 1.6 1.7
1.7 1.5 1.5 1.3 1.7 1.5
2 1.8 2.3 1.9 1.9 1.9
1.6 1.6 1.8 1.4 1.5 1.5
1.9 2 2.2 2.3 1.5 1.5
1.8 1.7 1.8 1.9 1.4 1.4
1.8 1.6 1.7 1.6 1.5 1.4
1.7 1.6 1.8 1.7 1.3 1.8
1.8 1.5 1.7 1.6 1.4 1.7
1.8 1.4 1.8 1.6 1.3 1.5
*Reference range: 0.8 – 1.5 mg/dL [10]. Table 19 Blood Alkaline phosphatase concentrations (U/L)* in jumping horses subsequent to exercise and a plasmapheresis session.
Horse
D-2 S1 S2
Time (Days / Samples) D-1 D0 D1 S3 S4 S5 S6 S7 S8
Control Group
ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO
158 84 117 110 117 110
162 98 141 124 141 112
141 88 127 141 127 112
184 88 144 142 144 142
145 86 140 140 140 111
174 112 154 142 136 125
174 102 150 130 143 124
159 96 132 129 141 124
158 95 139 123 139 123
159 93 140 110 140 110
160 93 141 101 122 118
Plasmapheresis Group
BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
100 131 110 117 161 116
104 139 113 141 139 141
140 88 111 127 160 127
156 156 117 144 142 144
135 136 115 140 159 140
140 177 119 147 140 126
164 141 117 123 148 133
137 141 112 111 171 123
136 132 111 111 165 111
132 129 112 110 169 112
129 121 110 110 146 110
Group
208 209 210 211 212 213
DALILANO
D2 D3 D4 S9 S10 S11
*Reference value : < 250U/L [5].
Table 20 Blood Gama Glutamyl Transferase concentrations (U/L)* in jumping horses subsequent to exercise and a plasmapheresis session.
Group
Control Group
Horse
D-2 S1 S2
Time (Days / Samples) D-1 D0 D1 S3 S4 S5 S6 S7 S8
ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO
10 10 11 10 11 10
10 10 14 10 14 10
10 10 15 10 15 10
10 10 18 10 18 10
10 10 18 10 18 10
10 10 22 10 11 13
10 10 17 10 10 10
10 10 16 10 10 10
10 10 10 10 10 10
10 10 12 10 12 10
10 10 13 10 10 10
BELUC
10 10 10
10 10 10
10 10 11
10 10 12
10 10 10
10 15 13
10 10 10
10 13 10
10 12 10
10 12 10
10 12 10
Plasmapheresis CHADDY Group PRINTANIER
D2 D3 D4 S9 S10 S11
DAOUDIYA CAMELIA DOUALIYA
214 215 216 217 218
15 13 10
18 11 18
18 12 18
13 10 11
14 10 11
11 10 10
11 11 10
10 11 10
10 10 10
Table 21 Blood Total bilirubin concentrations (mg/dL)* in jumping horses subsequent to exercise and a plasmapheresis session.
Horse
D-2 S1 S2
Time (Days / Samples) D-1 D0 D1 S3 S4 S5 S6 S7 S8
Control Group
ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO
2.2 1.7 1.2 1.6 1.2 1.6
2.5 3.8 1.7 1.8 1.7 1.8
1.9 1.6 1.2 1.9 1.2 1.9
2.5 1.7 1.5 2.6 1.5 2.6
2.4 1.6 1.2 2.2 1.4 1.6
2.3 1.6 1.2 3.2 1.2 3.2
2.3 2.3 1.3 1.8 2.6 1.9
2.2 2.1 1.6 1.9 2.3 1.2
2.2 2 1.7 1.5 1.7 1.5
2.4 1.8 1.5 1.6 1.5 1.6
2.3 1.3 1.4 1.4 1.7 1.2
Plasmapheresis Group
BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
3.1 1.7 0.7 1.2 1.6 1.6
1.9 1.9 2.1 2 2.3 2.45
3.2 1.6 1.9 1.2 1.4 1.5
3.7 1.8 2 1.5 1.6 1.5
3.5 1.6 2.1 1.2 1.5 1.2
3.2 2 2.1 1.7 1.7 1.6
3.1 2.1 2.2 1.8 1.6 1
2.6 1.6 1.7 1.7 1.6 1.7
2.1 1.7 1.6 1.6 1.6 1.7
2.3 1.8 1.5 1.3 1.5 1.7
1.5 1.6 0.8 1.1 1.8 1.6
D2 D3 D4 S9 S10 S11
*Reference value: 0.5 – 2.1 mg/dl [10]. Table 22 Blood Sodium concentrations (mmol/L)* in jumping horses subsequent to exercise and a plasmapheresis session.
Horse
D-2 S1 S2
Time (Days / Samples) D-1 D0 D1 S3 S4 S5 S6 S7 S8
Control Group
ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO
138 146 138 138 138 138
143 139 146 143 146 151
138 139 142 138 142 138
144 139 153 142 153 142
143 138 142 139 142 136
143 144 146 149 149 140
138 140 140 139 138 139
138 138 138 138 146 139
142 143 142 129 142 136
143 145 146 121 146 138
144 144 144 123 142 140
Plasmapheresis Group
BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
136 143 145 138 142 148
146 142 149 145 145 146
146 139 144 142 140 141
146 144 145 146 146 146
142 139 146 145 139 146
147 146 145 148 145 144
148 150 149 148 147 147
144 146 145 147 146 146
141 143 145 142 143 145
139 139 149 139 140 149
133 137 150 138 148 141
Group
224 225
14 12 10
*Reference value : < 30 U/l [5].
Group
219 220 221 222 223
11 10 11
*Reference range: 132-142 mmol/L [11, 12].
D2 D3 D4 S9 S10 S11
226 227 228
Table 23 Blood Potassium concentrations (mmol/L) * in jumping horses submitted to plasmapheresis after graded exercise.
Horse
D-2 S1 S2
Time (Days / Samples) D-1 D0 D1 S3 S4 S5 S6 S7 S8
Control Group
ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO
4.4 4.4 3.6 4.2 3.6 4.2
4.6 4.7 4.3 4.6 4.5 4.6
4.3 4.4 4.1 4.5 4.1 4.5
4.7 4.5 4.2 4.6 4.2 4.6
4.2 4.1 4.4 4.7 4.4 4.7
4.7 4.3 4.8 4.9 5.2 5.1
4.6 4.1 4.3 4.5 5.1 5
4.5 4.3 3.9 3.5 4.6 4.8
4.7 4.4 3 3.5 4.6 3
3.5 4.5 3.2 3.8 4.5 3.2
3.1 4.8 3.3 3.9 3.1 3
Plasmapheresis Group
BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
4.4 3.9 4.2 3.8 4 4.2
4.7 4.3 4.8 4.6 4.6 4.5
4.7 4.2 5.3 4.2 3.6 4.2
4.6 4.1 5.1 4.6 4.3 4.2
4.2 3.9 4.8 4.4 4.2 4.4
4.9 4.8 5.2 5.5 3.5 4.4
4.1 4.1 4.1 4.2 3.6 4.1
4.9 4.3 3.1 3.4 4.7 3.1
4.3 4.2 3.6 3.3 3.6 3.3
4.2 4.4 3.2 3.3 3.6 3.2
4.2 4.5 2.7 2.2 3.8 4.1
Group
229 230 231 232 233 234
*Reference range: 3.8 - 5.2 mmol/L [9].
Table 24 Blood Calcium concentrations (mmol/L) (mg/dL)* in jumping horses subsequent to exercise and a plasmapheresis session. Time (Days / Samples) D-1 D0 D1 S3 S4 S5 S6 S7 S8
Horse
D-2 S1 S2
Control Group
ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO
11 12 11.6 11.9 12 11 11.3 12 11.6 11.3 12.6 11.2 11.9 12.3 11.6 11.3 11.5 11
12 11.9 12.1 11.6 12.1 11.4
11.3 11.3 11.2 11.4 11.3 11.4
12.2 11 11.8 12.3 12.2 11.7
11.9 11.6 10.9 11.6 11.9 11.3
11.3 11.9 11.6 11.1 11.8 11.6
11.8 11.6 11.5 11.8 11.5 11.8
11.9 11.7 11.9 11.3 11.9 11.3
11.9 11.8 12 11.3 11.2 11.4
Plasmapheresis Group
BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
11.7 12 11.6 11.3 11.6 11.3 11 12.2 11 11.5 12 11.2 10.5 11.3 10.8 11.3 12 11.2
12 12 12.3 12.1 12.3 12.3
11.6 11.5 11.4 11.3 11.1 11.3
11.9 12.3 11.9 12.3 12 12.3
11.5 10.4 11.3 11.4 10.9 10.3
11.5 10.6 11.8 11.9 10.7 11.6
11.5 10.5 11.8 11.6 10.8 11
11.6 11.2 10.6 11.5 10.6 11.8
11.7 11.3 10.8 11.2 11.2 11.2
Group
235 236 237 238 239 240
D2 D3 D4 S9 S10 S11
D2 D3 D4 S9 S10 S11
*Reference range: 10.8-12.9 mg/dL [10].
Table 25 Plasma albumin (g/L)* changes in jumping horses subsequent to exercise and a plasmapheresis session.
Time (Days / Samples) Horse
D-2 S1 S2
D-1 S3 S4
Control Group
ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO
34 36 35 32 35 33
37 41 39 39 39 39
32 34 36 32 36 32
37 37 37 38 37 38
Plasmapheresis Group
BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
34 36 31 35 36 31
38 38 34 39 32 39
35 34 32 36 37 29
38 39 35 37 30 37
Group
241 242 243 244 245
33 36 38 33 38 33
36 38 38 39 38 38
35 35 32 38 35 38
36 42 36 39 31 33
S7
D1 S8
D2 D3 D4 S9 S10 S11
36 38 38 37 35 36
33 35 38 36 37 32
34 33 33 35 36 35
35 33 35 37 36 36
33 34 37 29 35 30
30 32 26 28 26 25
34 36 32 35 34 31
35 36 31 34 35 31
36 38 32 33 32 32
34 33 32 34 32 32
*Reference range: 26-37g/L [5]. Table 26 Plasma globulin (g/L)* changes in jumping horses subsequent to exercise and a plasmapheresis session.
Horse
D-2 S1 S2
Time (Days / Samples) D-1 D0 D1 S3 S4 S5 S6 S7 S8
Control Group
ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO
33 31 34 28 34 28
36 38 37 37 37 37
31 29 33 31 33 31
35 30 35 35 35 35
31 31 33 34 33 34
36 35 38 36 38 38
33 31 39 38 39 32
32 31 34 35 33 33
33 32 33 34 32 32
34 34 32 36 33 32
35 32 33 37 32 33
Plasmapheresis Group
BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
34 25 30 34 30 34
35 42 33 37 32 37
34 29 33 33 32 36
38 46 35 35 31 35
33 36 30 33 39 33
30 47 35 38 32 41
28 23 26 32 27 32
30 28 30 30 39 34
33 29 34 29 34 29
36 26 33 27 33 27
31 29 34 26 35 28
Group
246 247 248 249 250
S5
D0 S6
D2 D3 D4 S9 S10 S11
*Reference range: 25–45 g/L [5]. Table 27 Changes in Plasma total protein concentrations (g/L)* in jumping horses subsequent to exercise and a plasmapheresis session.
Group
Horse
Control Group ACOLITO
D-2 S1 S2
Time (Days / Samples) D-1 D0 D1 S3 S4 S5 S6 S7 S8
D2 D3 D4 S9 S10 S11
68
63
63
73
72
64
72
70
67
62
63
Plasmapheresis Group 251 252 253 254 255 256
74 72 64 70 66
79 76 76 76 76
73 71 65 74 66
67 76 78 76 78
73 71 65 70 65
73 76 75 76 74
72 76 72 70 70
71 73 66 73 67
71 73 67 74 62
71 69 68 73 64
71 70 68 73 63
BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
68 80 61 69 75 65
73 80 67 76 62 76
69 63 64 74 76 65
76 85 68 76 61 76
68 65 62 71 74 71
66 89 71 77 63 74
66 64 70 68 64 63
67 66 72 70 73 66
67 68 68 72 68 68
65 69 70 68 74 68
69 65 74 69 73 68
*Reference range: 62.5 – 75.0 g/L [13].
Table 28 Changes in blood aspartate Amino-transferase concentrations (U/L)* in jumping horses subsequent to exercise and a plasmapheresis session.
Horse
D-2 S1 S2
D-1 S3 S4
Time D0 S5 S6 S7
Control Group
ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO
242 311 310 192 310 192
275 350 365 251 365 251
276 293 352 254 375 259
285 331 388 263 388 263
283 350 365 255 345 350
304 359 391 267 352 362
302 354 380 282 370 378
287 338 378 256 369 380
279 336 344 246 355 360
275 334 345 232 345 346
274 332 348 226 334 325
Plasmapheresis Group
BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
165 231 300 310 357 335
348 245 310 365 319 365
212 275 325 372 359 375
215 289 349 388 312 388
219 296 358 376 345 389
223 309 359 387 355 400
230 221 295 289 201 230
219 227 315 299 221 238
230 233 321 304 232 256
223 236 305 301 226 306
240 220 318 302 210 235
Group
257 258 259 260 261
DAHBI CHAMAA DAHMAN DAMAA DALILANO
D1 S8
D2 D3 D4 S9 S10 S11
*Reference range: 160-412 U/L [11, 12]. Table 29 Variations of blood creatine kinase concentrations (U/L)* in jumping horses subsequent to exercise and a plasmapheresis session.
Group
Control Group
Horse
D-2 S1 S2
Time (Days / Samples) D-1 D0 D1 S3 S4 S5 S6 S7 S8
ACOLITO DAHBI CHAMAA DAHMAN DAMAA
165 150 149 112 149
172 151 156 212 156
180 160 230 210 169
182 168 268 250 162
136 152 179 172 179
135 175 231 205 201
274 165 133 189 200
212 166 154 212 189
D2 D3 D4 S9 S10 S11 182 165 150 201 169
174 165 136 196 156
169 153 152 189 146
Plasmapheresis Group 262 263 264 265 266
123
215
212
262
172
250
212
236
226
212
189
BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
120 122 123 123 306 149
160 201 151 146 315 212
125 191 128 156 302 146
189 203 159 156 332 232
153 135 132 179 151 179
159 183 149 196 220 202
105 109 110 123 132 132
111 102 103 102 103 123
110 112 114 123 114 112
110 120 117 120 116 115
112 121 120 120 123 123
*Reference range: 60-330 U/L [11 , 12]. Table 30 Changes in blood lactate concentrations (mmol/L)* in jumping horses subsequent to exercise and a plasmapheresis session.
Horse
D-2 S1 S2
Time (Days / Samples) D-1 D0 D1 S3 S4 S5 S6 S7 S8
Control Group
ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO
1 1.2 1.1 1 1.1 1
13.7 15.2 15.4 14.8 18.2 18.6
1.1 1.2 1 1 1.1 1.1
13.5 14.2 16.9 14.6 19.6 20.1
1 1.3 1 1 1.2 1.1
11.4 12.6 17.8 14.7 20.9 19.7
2.4 2.2 3.4 3.1 4.1 3.9
1 1.2 1.1 1.1 1.2 1.1
1 1.3 1.1 1 1.1 1.1
1 1.2 1 1 1.1 1
1 1.3 1.1 1.1 1.1 1.1
Plasmapheresis Group
BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
1 1.1 1 1 1.2 1.2
13.2 13.5 23.4 14.7 19.6 17.9
1 1 1.3 1 1.1 1.1
13.3 16 21.9 14.9 20.8 18.1
1 1 1.2 1 1 1.2
14.5 14.3 24.5 15.1 21.1 18.4
1.1 1.2 1.3 1.1 1.1 1.1
1 1.1 1.1 1 1.1 1
0.9 1.1 1.1 0.8 1 0.9
0.9 1.1 0.9 0.8 1 0.8
0.9 1 0.9 0.9 1 0.9
Group
267 268 269 270 271
DALILANO
D2 D3 D4 S9 S10 S11
*Reference value : < 2.5 mmol/L [9]. 2. Experimental Design, Materials, and Methods Investigations were carried out on twelve show jumping horses, they randomly allocated to two
272
groups: a plasmapheresis group (n=6) and a control group (n=6). All horses were healthy and
273
received the same food diet three times a day. First in the morning, they were offered each 1kg
274
of commercial feed (Destrier, France). Then, in midday, they received each 2.5kg of barley and
275
its bran (0.5kg) in addition to 30g of a vitamin and mineral supplement. Finally, in the evening,
276
horses were fed each 0.5kg of oats and 2kg of barley. A supplement of 2.5kg of alfalfa hay was
277
given for each horse after each food ration distribution. Water was provided ad-libitum.
278
Horses of both groups underwent each morning an exercise of 30 min/day, during 3 successive
279
days (Day -2, -1 and 0). Blood was sampled before and after exercise. Following the 3rd day
280
exercise (Day 0) and its corresponding subsequent blood sampling, a plasmapheresis session
281
was performed on the horses of the plasmapheresis group while horses of the control group
282
were maintained in the same experimental environment but without being subjected to
283
plasmapheresis. Then blood was sampled from each horse of both groups. Subsequent daily
284
blood samples were taken at the same hour for the following 4 days (Day 1, 2, 3 and 4). All
285
blood samples were obtained using jugular venipuncture. Sampling was realized in the morning
286
between the two first food diets of the day.
287
In order to verify the effect of plasmapheresis, the physical exercise executed by horses
288
subjected to horse should be capable to have to comply the condition of inducing a significant
289
raise of several hematobiochemical parameters and specifically providing moderate to high
290
values of lactates. The well-known triangular exercise carried out in the trotter horses by
291
Demonceau and Auvinet [1] [2] have been first used. This exercise corresponds to succession
292
of warm-up period (15 minutes) and 11minutes of workloads exercise (steps) and then a
293
recovery period of 10 minutes. Data of lactate concentrations obtained from blood samples
294
taken 5 to 10 minutes after exercise test finished varied from 2.0 and 3.7 mmol/L (Figure 1B).
295
To obtain high values of lactates, the previous exercise test was modified by increasing the
296
duration of the warm-up phase up to 30min and steps were substituted by gallop. The new
297
warm-up stage consisted on 5min trot, 3min of gallop, 5min of trot, 3min of gallop, 5min trot,
298
3min of gallop and finally 6min of trot. The speeds of horses at trot was 240 m/min while it
299
was 350 m/min for gallop. Data of blood lactate concentrations increased by 6 times (Figure
300
1B) varying between 13.2 and 23.4mmol/L. This new exercise was then used in the following
301
experiment investigating the effect of plasmapheresis.
302
303
Figure 1. Lactate concentrations in six jumping horses. (A): Before and following the
304
exercise test standardized by Demonceau and Auvinet. (B): Before and following the
305
modification of the warm-up phase of the same exercise test. The duration of this phase was
306
doubled to 30 min instead of 15 min and steps were substituted by gallop.
307 308
Plasmapheresis was performed by harvesting of 7L of plasma per horse which are replaced by
309
7L of NaCl 0.9%. The technique was performed by using a small-sized plasmapheresis
310
machine commercialized by Hemofenix-France utilizing Trackpore technology (Dubna
311
Moscow region-Russian Federation). The machine is equipped with Rosa® type membrane
312
filters with 0.4µm pores. The system is totally automated operating.
313
Haematological parameters included the number of red blood cells (RBCs), hemoglobin
314
concentration and hematocrit, the mean corpuscular volume (MCV), the mean corpuscular
315
hemoglobin (MCH), the mean corpuscular hemoglobin concentration (MCHC), the number of
316
platelets and also the number of white blood cells (WBCs) and the count of lymphocytes,
317
monocytes, eosinophils and granulocytes. All these parameters were determined using a
318
veterinary cell-counter automate, the Celltac VET MEK-6550 hematology (Nihon Kohden,
319
Tomioka-Japan) and its reagents, Hemolynac·3 MEK-660I, Isotonac·4 MEK-641I, Cleanac
320
MEK-520I (Celltac VET MEK-6550 J/K hematology, Nihon Kohden, Tomioka-Japan).
321
Concerning the biochemical parameters, the concentrations of albumin, globulin, total protein,
322
glucose, alkaline phosphatase (ALP), aspartate aminotransferase (AST), gamma glutamyl
323
transferase (GGT), total bilirubin, lactate, creatinine kinase (CK), urea, creatinine, calcium,
324
sodium and potassium were determined using the veterinary Skyla-VB1 automate analyzer
325
(Lite-On Technology Corporation, Hsinchu-Taiwan). All the chemical reactions completed
326
inside a circular and transparent plastic reagent disc (Equine Panel- Product code 900-150,
327
skylaTM VB1 reagent disc, Hsinchu-Taiwan), containing different reaction cuvettes with
328
specific assay reagent for each measured parameter. Concentrations quantified by photometric
329
measurement of the absorbance changes arising from the chemical reactions in cuvettes’ disc
330
are automatically given by the analyzer.
331
Lactate concentration was assayed using the portable analyzer Lactate-Pro™ 2. This test meter
332
is based on a reaction of the blood lactate with a reagent in the Lactate-Pro™ 2 Test Strip
333
(Busimedic®, S.L, San Sebastián-Spain). To validate and to ensure the accuracy of this
334
technique, blood lactate concentrations measured using the Lactate-Pro™2 were compared to
335
those measured by a reference analyzer, COBAS™ Gen.2 using the reagent COBAS
336
INTEGRA Lactate Gen.2 (Roche®, Maylan-France). These analyses were conducted on
337
specimens of six Athlete show jumping horses having performed the developed exercise, as
338
previously described. Blood was sampled before and after the exercise and lactate
339
concentration was immediately assayed by Lactate-Pro™2 and COBAS™ Gen.2 analyzers.
340
Measurements from both instruments were tested for correlation.
341 342
For further information concerning the experimental procedures please see the research
343
article [2][1]accompanying this data paper.
344 345 346 347
Acknowledgments Authors of this research work express sincere recognition to the Moroccan Royal Guard, the
348
Hassan II Institute of Agronomy and Veterinary Medicine (IAV Hassan II), the Royal Horse
349
Encouragement Society (SOREC), the Society for the Protection of Animals and Nature
350
(SPANA-Morocco), GIGA-LAB Morocco and finally Hemofenix France, funding this
351
research.
352 353 354 355 356 357 358 359 360 361 362 363 364 365 366
References [1] Daden R., Zarhouni F. Z., Chakir J., Piro M., Achaaban M.R., Ouassat M. & El Allali K. Plasmapheresis effect on haematological and biochemical parameters in athletic horses subjected to exercise. https://doi.org/10.1016/j.jevs.2019.07.006. [2] Demonceau T, Auvinet B. Exercise test for trotters in training: practical realization and first results. In: Report of the 18th Study Day. CEREOPA 1992; 120–132. Paris. [3] Marlin D., Nankervis K. (2013). Equine Exercice Physiology. Edition by Blackwell Publishing Company. [4] Huntington.P , Myers.J and Owens.E . (2004). Horse Sense : The Guide to Horse Care in Australia and New Zealand. Second edition , Ch 6 p:176. [5] Orsini A. and Divers J. (2001). Urgences en médecine équine, Traitement et procédures. MALOINE edition. 834p
367 368 369 370 371 372 373 374 375 376 377 378 379 380 381 382 383 384 385 386 387 388 389 390 391 392 393 394 395 396 397 398
[6] Grondin T.M., Dewitt S.F. Normal Hematology of the Horse and Donkey. In: Weiss D.J., Wardrop K.J. (dir.) (2010). Schalm´s Veterinary Hematology. Ames, Iowa, USA: Wiley Blackwell, pp. 821-828. [7] Desjardins I., Cadoré J.L. (2006). Analyses sanguines équines. I – Hématologie: approche Clinique. Pratique Vétérinaire Equine, 38(151), pp. 9-22. [8] Weiser G., Thrall M.A. Introduction to Leukocytes and the Leukogram. In: Thrall M.A., Baker D.C., Campbell T.W., Denicola D., Fettman M.J., Lassen E.D., Rebar A., Weiser G. (dir.) (2004). Veterinary Hematology and Clinical Chemistry. Baltimore, MD: Lippincott Williams & Wilkins, pp. 125-130. [9] Jaillardon. L (2017) . Petit memento de biochimie. Laboratoire ONIRIS. Site de la Chantrerie. Nantes. [10] Cornell University. Cornell University College of Veterinary Medicine [en ligne]. URL: https://ahdc.vet.cornell.edu/sects/clinpath/reference/chem.cfm (viewed on 17th August 2019 ) [11] Rose R. et Hodgson D. (1999). Hematology And Biochemistry. Dans R. H. Rose, The Athletic Horse. (Pp. 63-78). Saunders. [12] Smith BP. (2002). Large Animal Internal Medicine : diseases of horses, cattle, sheep and goats., 3th edition. St Louis : Mosby Cie,:1-1735. [13] Soares, J.C.M., Zanella, R., Bondan, C., Alves, L.P., Ragagnin de Lima, M., Costa da Motta, A., Zanella, E.L. (2011) Biochemical and antioxidant changes in plasma, serum, and erythrocytes of horses before and after a jumping competition. Journal of Equine Veterinary Science, 31, 357-360.
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46
S2352-3409(19)31008-X
DOI:
https://doi.org/10.1016/j.dib.2019.104653
Reference:
DIB 104653
To appear in:
Data in Brief
Received Date: 22 July 2019 Revised Date:
31 August 2019
Accepted Date: 3 October 2019
Please cite this article as: R. Daden, S. Chbihi, F.Z. Zarhouni, J. Chakir, M. Piro, M.R. Achaaban, M. Ouassat, K. El Allali, Dataset concerning haematological and biochemical parameters changes in show jumping horses subjected to exercise and plasmapheresis session, Data in Brief, https://doi.org/10.1016/ j.dib.2019.104653. This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. © 2019 The Author(s). Published by Elsevier Inc.
1 2 3 4
Article Title Dataset concerning haematological and biochemical parameters changes in show jumping horses subjected to exercise and plasmapheresis session Authors
5
Réda Dadena,b, Sara Chbihia, Fatima Zahraa Zarhounia, Jamal Chakirc, Mohammed Pirod,
6
Mohamed Rachid Achaabana, Mohammed Ouassata& Khalid El Allalia.
7 8 9
Affiliations a
Comparative Anatomy Unit/URAC49, Department of Biological and Pharmaceutical
10
Veterinary Sciences, Hassan II Agronomy and Veterinary Medicine Institute, Rabat, Morocco.
11
b
Jumenterie de Zouada, Veterinary Hospital- Royal Guard, Tetouan-Morocco.
12
c
Veterinary Hospital- Royal Guard, Rabat-Morocco, Morocco.
13
d
14
Veterinary Medicine Institute, Rabat, Morocco.
15 16
PMC- EC, Department of Medicine, Surgery and Reproduction, Hassan II Agronomy and
Corresponding author
17
Khalid El Allali
18
Address: Hassan II Agronomy and Veterinary Medicine Institute,
19
B.P.6202 Rabat-Instituts, 1010, Rabat-Morocco.
20
Phone numbers: 00 212 6 61 14 04 70
21
Fax numbers: 00 212 0537 77 58 45
22
E-mail adresse : [email protected]
23
Abstract
24
This article presents data on the effect of plasmapheresis on clinical, haematological and
25
biochemical parameters in horses following exercise and after a plasmapheresis session. This
26
later was realized
27
(plasmapheresis group) that underwent three consecutive days of physical graded exercice
28
graded physical exercise. The control group (n=6) went through the same exercise but was not
This blood filtration technique was realised on six jumping horses
29
subjected to the plasmapheresis session. Blood was sampled before and after each exercise, and
30
also at the beginning and the end of plasmapheresis session. The presented data was obtained by
31
measuring clinical and haemato-biochemical parameters in both groups. The heart and
32
respiratory rates and rectal temperature were recorded. In addition, the number of red blood and
33
white cells, platelets and also of lymphocytes, monocytes, eosinophils and granulocytes were
34
counted. Other haematological parameters including, hemoglobin concentration, hematocrit,
35
mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin
36
concentration were determined. Concerning the biochemical parameters, the concentrations of
37
albumin, globulin, total protein, glucose, alkaline phosphatase, aspartate aminotransferase,
38
gamma glutamyl transferase, total bilirubin, lactate, creatinine kinase, urea, creatinine, calcium,
39
sodium and potassium were measured. All parameters data were analyzed by a two-way repeated
40
measures analysis of variance followed by Holm-Sidak post-hoc procedure to evaluate the effect
41
of plasmapheresis and time. This paper contains data related to and supporting research articles
42
currently published entitled "Plasmapheresis effect on haematological and biochemical
43
parameters in athletic horses subjected to exercise" (Daden et al, 2019) [1].
44 45
Keywords
46 47 48 49 50
Plasmapheresis, horse, exercise, haemato-biochemical parameters.
Specifications Table Subject
Veterinary Science and Veterinary Medicine
Specific subject area
Equine medicine, Equine exercise, Exercise physiology, Blood filtration technique, Plasmapheresis, Haematology Biochemistry
Type of data
Tables, Figure
How data were acquired
-Physiological parameters: Physiological parameters were measured in the two groups using the same procedures as in clinical examination. • Heart rate: the pulse on the facial artery was taken. • Respiratory rate: Horse's chest movements were counted.
•
Rectal temperature: recorded using a digital thermometer (range, 32– 42°C; accuracy, 0.1°C). -Plasmapheresis: was performed by using a Hemofenix® plasmapheresis machine commercialized by Hemofenix-France utilizing Trackpore® technology (Dubna Moscow region-Russian Federation). -Blood sampling: Blood samples were obtained by jugular venipuncture and were collected in BD-Vacutainer EDTA tubes for haematological parameters and in lithium heparin’BD-Vacutainer tubes for biochemical parameters. -Haematological parameters analysis was performed by using a veterinary cell-counter automate, the Celltac VET MEK-6550® haematology (Nihon Kohden, Tomioka-Japan) and its reagents, Hemolynac·3 MEK-660I, Isotonac·4 MEK-641I, Cleanac MEK520I (Celltac VET MEK-6550 J/K haematology, Nihon Kohden, Tomioka-Japan). -Biochemical parameters were assayed using the veterinary SkylaVB1® automate analyzer (Lite-On Technology Corporation, Hsinchu-Taiwan) and reagent disc (Equine Panel- Product code 900150, skylaTM VB1® reagent disc, Hsinchu-Taiwan). -Lactate concentrations were determined using Lactate-Pro™ 2 analyzer (Busimedic®, S.L, San Sebastián-Spain). Data format
Raw Data
Parameters for data collection
The data were obtained from 12 show jumping horses that were divided into plasmapheresis (n=6) and control (n=6) groups. Each group was formed by contained 3 females and 3 males of AngloHispano-Arab breed, aged from 4 to 12 years and weighing 400kg. Throughout the study, horses were healthy and their clinical state was rigorously controlled.
Description of data collection
All horses underwent the same daily physical exercise for three consecutive days. Following the 3rd day exercise, a plasmapheresis session was performed only on plasmapheresis group horses horses of plasmapheresis group. All physiological and haematobiochemical parameters data presented in this article were obtained from blood samples taken simultaneously on horses of the plasmapheresis and control groups before and after each exercise and after plasmapheresis session for the following 4 days.
Data source location
Institution: Royal Guard of Tétouan City/Town/Region: Tétouan Country: Morocco Latitude: 35.5730287 Longitude : -5.3515084 GPS Coordinates: 35° 34' 22.904" N 5° 21' 5.429" W
Data accessibility
Raw Data is available with this article
Related research article
Author’s name: Réda Dadena,b, Fatima Zahraa Zarhounia, Jamal Chakirc, Mohammed Pirod, Mohamed Rachid Achaabana, Mohammed Ouassata & Khalid El Allalia. Title: “Plasmapheresis Effect on Haematological and Biochemical Parameters in Athletic Horses Subjected To Exercise” Journal: Journal of Equine Veterinary Science DOI: https://doi.org/10.1016/j.jevs.2019.07.006
51 52 53
Value of the Data
54 55 56 57 58 59 60 61 62 63 64 65
•
These data highlight the effect of plasmapheresis and demonstrated that this blood filtration technique is able to modify the physiology post-exercise of the horse and to affect both the haematology and the biochemistry of the blood.
•
This data represents for the first time the changes in physiological, haematological and biochemical parameters in horses subjected to a plasmapheresis session after three days of exercise. It can be thus taken into consideration by other researchers interested in both sport physiology and blood filtration techniques field.
•
Such data can be used as a reference series for comparative approaches in the field. By providing relevant information, it allows a reuse and a better interpretation of the results. This is also useful for future studies.
66 67
•
The data demonstrate for the first in horse that plasmapheresis could be considered a s a doping technique.
•
In addition, the present data show informations that are complementing the related article and can be interesting for readers of the Data in Brief journal.
68 69 70 71
72 73 74
Data shown in this article provide information about the effect of plasmapheresis on
75
physiological, haematological and biochemical parameters in athletic horses subjected to
76
exercise. Horses of both plasmapheresis and control groups underwent each morning an exercise
77
of 30 min/day, during 3 successive days (Day -2, -1 and 0). Following the exercise session of the
78
3rd day (Day 0) a plasmapheresis session was performed only on the horses of the plasmapheresis
79
group. The control group were maintained in the same environment but without being subjected
80
to plasmapheresis. Data of haemato-biochemical parameters were obtained by analyzing blood
81
samples collected before and after exercise and plasmapheresis. Tables 1, 2 and 3 describe
82
clinical parameters variations subsequent to an exercise and a plasmapheresis session. Tables 4
83
to 10 represent each one parameters of red hemogram before and after exercise also before and
84
after a plasmapheresis session. Tables 11 to 15 describe parameters of white hemogram and their
85
changes subsequent to exercise and plasmapheresis session. Tables 16 to 30, represent variations
86
of biochemical parameters before and after graded exercise and plasmapheresis session.
87
Figure 1 illustrate Lactate concentrations before and following the exercise test standardized by
88
Demonceau and Auvinet [2], also before and following the modification of the warm-up phase of
89
the same exercise test.
90 91 92
1. Data
1.1.Tables
93
The following tables containing raw data concerning changes in physiological, heamtological
94
and biochemical parameters of horses subjected to subsequent to graded physical exercise and
95
plasmapheresis session. Data of each sample are presented individually for animals of the control
96
and plasmapheresis groups.
97
measurements after the exercise; S7: immediate measurement after the plasmapheresis session;
98
S8, S9, S10, S11: post-plasmapheresis measurements at rest. D-2: 48h before plasmapheresis D-
99
1: 24h before plasmapheresis; Day 0: day of plasmapheresis subsequent to the last exercise; D1,
100 101 102 103 104
S1, S3, S5: measurements before the exercise; S2, S4, S6:
D2, D3 and D4 coresspond to 24h, 48h, 72h and 96h respectively after plasmapheresis.
105 106 107
Table 1 Rectal temperature (°C)* variations in jumping horses subsequent to an exercise and a plasmapheresis session.
Horse
D-2 S1 S2
Time (Days / Samples) D-1 D0 D1 D2 D3 D4 S3 S4 S5 S6 S7 S8 S9 S10 S11
Control Group
ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO
37.5 37.2 37.8 37.6 37.5 37.8
40.1 40 40.6 39.9 39.9 41
37.5 37.8 37.6 37.6 37.6 37.6
40.2 40.8 40.6 39.9 40 39.9
37.5 37.5 37.5 37.6 37.6 37.6
40.3 40.9 40.5 40.1 40.1 40.1
38.1 37.5 38.1 38.2 38.3 38.2
37.5 38.3 37.9 37.5 37.6 37.5
37.8 38.6 37.6 37.5 37.5 37.5
37.6 38.5 37.5 37.5 37.5 37.5
37.5 38.6 37.6 37.5 37.6 37.5
Plasmapheresis Group
BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
37.6 37.5 37.7 37.7 37.6 37.8
39.9 40.8 40.9 40.6 40.6 39.9
37.6 37.5 37.8 37.8 37.6 37.9
39.9 40.9 40.8 41 40.6 40.2
37.6 37.5 37.7 37.8 37.5 37.5
40.1 40.9 40.9 40.9 40.5 40.1
38.2 37.5 37.9 40.1 38.1 39.1
37.5 38.3 37.8 37.9 37.9 38.2
37.5 38.6 37.8 37.8 37.6 37.5
37.5 38.5 37.9 37.8 37.5 38.5
37.5 38.6 37.8 37.8 37.6 39.5
Group
108 109 110 111 112
*Normal rectal temperature of adult horse : 37.0 – 38.0 °C [3]. Table 2 Heart rate (beats per minute)* changes in jumping horses subsequent to an exercise and a plasmapheresis session.
Group
113 114 115 116 117
Horse
Control Group
ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO
Plasmapheresis Group
BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
Time (Days / Samples) D-1 D0 D1 D2
D-2 S1
S2
S3
S4
S5
S6
S7
S8
S9
36 36 32 36 36 36 36 36 45 41 32 38
112 120 120 110 120 115 110 104 167 110 110 112
36 36 36 36 36 36 36 36 44 44 36 40
116 126 132 115 132 110 113 100 162 120 120 132
36 36 36 36 36 36 36 36 44 44 36 42
120 123 123 110 152 110 100 120 152 118 114 110
38 36 38 36 36 36 36 36 46 45 38 40
36 36 36 36 36 36 36 36 44 44 36 36
36 36 36 38 38 38 38 36 45 42 36 38
D3
D4
S10 S11 38 36 38 36 36 36 36 36 46 44 38 38
36 36 36 36 38 36 36 36 44 40 36 36
*Normal heart rate of adult horse: 30 – 40 beats per minute [3]. Table 3 Respiratory rate (breaths per minute)* changes in jumping horses subsequent to an exercise and a plasmapheresis session. Time (Days / Samples)
Group
Control Group
Plasmapheresis Group 118 119 120 121 122 123 124
D-2 S1 S2
D-1 S3 S4
S5
D0 S6
S7
D1 D2 D3 D4 S8 S9 S10 S11
ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
24 20 20 20 20 20 20 24 28 28 24 24
26 24 24 28 28 28 28 20 32 28 24 28
26 20 20 24 24 24 24 24 28 26 20 24
68 72 66 66 66 68 66 68 72 68 68 66
28 24 24 28 28 28 28 24 24 28 24 28
24 28 24 24 24 24 24 28 28 26 24 24
68 68 68 64 64 66 64 64 98 64 68 68
68 64 62 68 68 68 68 72 68 63 63 68
24 24 20 22 22 22 22 24 28 24 20 28
24 28 24 24 24 24 24 28 28 24 24 26
24 24 24 24 24 24 24 24 28 24 24 24
*Normal respiratory rate of adult horse: 8 – 20 breaths per minute [4].
Table 4 Red blood cell count (106/µl)* changes in jumping horses subsequent to exercise and a plasmapheresis session.
Horse
D-2 S1 S2
Time (Days / Samples) D-1 D0 D1 D2 D3 D4 S3 S4 S5 S6 S7 S8 S9 S10 S11
Control Group
ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO
8.88 8.63 8.71 7.59 7.59 7.53
10.6 13.3 12.2 12.1 12.1 12.1
8.58 8.58 9.42 9.74 9.74 9.74
10.4 10.4 11.9 11.6 11.6 11.6
10.2 8.61 11.2 9.34 8.02 8.1
13.2 10.7 14.3 11.2 11.9 12.4
8.01 8.67 10 10.2 7.99 9.52
7.47 10.3 8.49 7.58 7.58 7.75
7.92 9.6 9.11 7.75 7.75 7.53
8.62 8.17 8.56 7.53 7.53 7.74
8.71 8.63 9.01 7.56 7.56 13.4
Plasmapheresis Group
BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
11.7 11.2 8.26 7.56 8.71 7.92
11.3 15.3 11.4 12.1 12.2 11.4
9.74 9.94 8.3 9.42 9.42 7.39
12.9 14.5 11.5 11.9 12.3 11.5
10.2 11.2 8.35 9.79 10.4 8.35
13.1 14.7 11.3 12.6 12.4 11.3
13.2 13.5 11.9 12.8 12.7 13
12.3 12.1 10.3 10.2 8.03 7.25
10.6 10.3 10.2 7.96 6.95 8
10.4 9.27 7.96 7.82 7.22 8.19
9.31 10.3 7.43 10.2 10.5 8.39
Group
125 126 127 128 129
Horse
*Reference range: 6.70 – 12.90.106 /µL [5]. Table 5 Changes in blood hemoglobinconcentrations (mg/dL) (g/dl)* in jumping horses subsequent to exercise and a plasmapheresis session.
Group
Horse
D-2 S1 S2
Time (Days / Samples) D-1 D0 D1 D2 D3 S3 S4 S5 S6 S7 S8 S9 S10
D4 S11
130 131 132 133 134 135 136 137
Control Group
ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO
14.2 13.3 13 11 11 11.1
16.9 19.3 17.3 18.2 15.6 18.2
13.7 13.2 14.1 15.5 11.5 15.5
17.5 16.7 17.4 17.6 14.3 17.6
11.7 13.2 14.5 15.8 11.6 15.8
17.4 16.8 17.8 16.2 14.1 18.1
13 13.5 12.9 12.1 11.3 11.8
12.3 15.8 12.9 11.3 12.7 10.9
13.2 14.6 13.4 10.9 11.3 11
14.1 12.7 13.2 11 11.9 11
14.3 13.4 13 10.8 12.7 18.9
Plasmapheresis Group
BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
17 15.2 12.2 10.5 13 10.8
17.7 24.4 17.3 18 17.3 17.3
15.5 13.4 12.1 14.1 14.1 10.3
19.4 19.2 17 17.4 17.4 17
15.3 14.3 12 14.5 16.8 12
19.8 19.7 16.8 17.1 17.5 16.8
19.6 19.2 17 19.2 17.5 17
14.6 13.9 12 12.8 12.2 12.3
14.8 13.5 12.2 12 12.3 11.9
15.6 12.5 11.8 10.7 10.8 12.6
13.8 14.5 11.1 13.6 15.8 12.2
*Reference range: 11.0 – 19.0 g/dl [6].
Table 6 Blood hematocrit (g/dL)* variations changes in jumping horses subsequent to exercise and a plasmapheresis session.
Group
Control Group
Plasmapheresis Group 138 139 140 141 142 143
Horse
D-2 S1 S2
Time (Days / Samples) D-1 D0 D1 D2 D3 D4 S3 S4 S5 S6 S7 S8 S9 S10 S11
ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
43.9 40.4 39.5 34 36.6 33 37.5 37.9 36.1 38.5 42.1 37
42.5 40.4 42.5 43.8 36.8 42.9 43.8 41.3 36.8 42.5 42.5 32.8
52.5 59.8 52.4 54.9 48.5 54.9 53.3 53 51.3 55.2 52.4 51.3
51.7 54 52.4 50 53 52 57.9 53 53 52.4 52.4 51.4
36.5 40.3 44.4 43 35.9 43 45.7 43 37 44.4 45 33.8
52.9 50.8 53.3 49 56.1 54.8 59.2 61.4 51.2 53.9 53 51.2
39.5 41.1 38.9 36.2 48.9 36 59 60.9 53 54 50.6 53
39.9 44.9 38.5 38.5 43.1 37.7 31.9 32.8 32.7 31.2 37.7 34.7
38 39 36 37.4 49 36 35.4 33.3 31.1 40.1 36.8 36.7
35.2 37.7 34.2 34.9 51.3 37 33.7 32.6 28.2 37.3 38.1 33
34 39.5 38.8 35.6 34.4 34.2 35.6 32.8 30.6 31.3 35.4 33.8
*Reference range: 32.0 – 53.0 g/dl [6].
Table 7 Changes in blood mean corpuscular volume (fL)* in jumping horses subsequent to exercise and a plasmapheresis session.
Group
Horse
Control Group
ACOLITO
D-2 S1 S2 44.8
46
Time (Days / Samples) D-1 D0 D1 D2 D3 S3 S4 S5 S6 S7 S8 S9 S10 45
46
44.6
45.2 44.8 44.6
44.4
44.5
D4 S11 44.6
Plasmapheresis Group 144 145 146 147 148
41.4 44.3 42.1 45 43.4 45.4 46.8 41.4 44.8 41.2 44.8
44 45.2 45.6 46.8 45.2 46 47 43 45.4 42.8 45.4
41.5 44.2 45.4 44.6 43 45.5 46.3 41.3 45 40.9 45
44.1 45 45.5 45.5 45.3 46.1 47.4 45.5 45.6 42.3 45.6
41.3 44.3 45.4 44.7 44.3 44 45 45.4 45.2 41.2 43
43 45.2 43 45.2 45.2 45.5 46 45.9 45.5 42.1 43.1
41.7 44.9 42.7 45.6 43.9 45.5 47.1 45.2 45.3 42.4 43.2
41.3 44.4 42.3 45.3 43 44 45 45.1 45.1 41.6 44.9
41.6 44.1 42.2 45 43.6 44.9 44 44.9 45 41.3 45
41.2 44.3 42.2 45.3 45.5 45 47.2 45.2 45 41.8 43
40.9 44.5 42.2 45.5 43.9 45 47 44.9 44.7 41.7 42.9
*Reference range: 37.0 – 59.0 fL [6]. Table 8 Changes in blood mean corpuscular hemoglobin (pg)* in jumping horses subsequent to exercise and a plasmapheresis session.
Group
Control Group
Plasmapheresis Group 149 150 151 152 153
DAHBI CHAMAA DAHMAN DAMAA DALILANO BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
Horse ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
D-2 S1 S2
Time (Days / Samples) D-1 D0 D1 S3 S4 S5 S6 S7 S8
D2 S9
D3 S10
D4 S11
14.9 13.5 14.8 14.5 14.9 14 15 15.6 14 14.5 13.9 14.5
14.9 13.8 14.7 14.7 14.7 13.8 15.2 15.4 14 14.6 13.5 14.3
14.8 13.6 15 13.9 15.1 14.1 14.9 15.6 14.3 14.5 13.6 14.6
14.8 13.5 15.1 13.7 15 14.6 15.2 15.5 14.9 14.6 13.6 14.2
14.8 13.2 14.9 13.4 15.1 15.1 15 15.5 14.5 14.8 13.8 14.1
15.7 15.4 15.2 14.9 15 15.2 16 16.2 14.1 15 14.1 15
15.1 15.5 15 15.1 15.1 15 16.2 16.1 14.6 14.9 14.2 15
14.9 13.1 14.4 14.8 14.7 14.4 15 15.5 14 14.4 13.9 14.5
15.1 15.2 14.8 15.3 15.4 15.2 16.3 15.7 15.3 14.7 13.8 14.6
14.8 14.5 14.4 14.2 15.3 14.7 16.2 15.8 15.4 14.9 13.5 14.8
14.5 13.8 14.9 14 15.2 14.6 15 15.4 14.3 14.6 13.9 14.5
*Reference range: 12.3 – 19.9 pg [7]. Table 9 Changes in blood mean corpuscular hemoglobin concentration (g/dL)* in jumping horses subsequent to exercise and a plasmapheresis session.
Group
Control Group
Plasmapheresis
Horse ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO BELUC CHADDY
D-2 S1 S2 32.3 32.9 32.9 32.4 32.9 32.4 32.4 32.7
33 33.3 33 33.2 33 33.2 33.2 33.2
Time (Days / Samples) D-1 D0 D1 S3 S4 S5 S6 S7 S8 32.2 32.7 32.8 32.3 32.8 32.3 32.5 33.2
33.8 33.9 33.1 33.1 33.1 33.1 33.5 33.2
32.1 32.8 32.7 32.4 32.7 32.4 32.5 32.6
32.9 33.1 33.4 33.1 33.4 33 33.4 33.1
32.9 32.8 33.1 33.4 33.2 33 33.1 33.6
33.4 32.8 32.5 32.7 32.5 32.9 32.4 33.6
D2 S9 33.1 33.2 32.8 32.5 32.1 33 32.3 33
D3 D4 S10 S11 32.8 33.1 32.8 32.6 32 33 32 32.7
32.5 33 32.9 32.6 33 32.6 32.5 32.1
Group
154 155 156 157 158 159
33.3 33.7 32.8 33 32.6 32.8 32.9 33 32.8 32.3 33.7 32.1
33.5 33.1 33.1 33.5
32.4 32.7 32.2 32.4
33.2 33.1 33.2 33.6
32.2 32.1 32 31.6 32.1 32 32.4 32.6 32.5 33.2 32 32.9
32.5 32.4 32.6 33
33.1 32.6 32.5 33.2
*Reference range: 31 – 38.6 g/dl [7].
Table 10 Blood platelet count (103/µl)* variations in jumping horses subsequent to exercise and a plasmapheresis session.
Horse
D-2 S1 S2
Time (Days / Samples) D-1 D0 D1 S3 S4 S5 S6 S7 S8
Control Group
ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO
172 152 125 143 152 143
179 146 170 127 170 127
171 146 136 143 149 143
193 165 198 119 198 119
155 148 134 141 134 141
173 145 164 119 180 114
164 149 133 131 128 95
143 135 147 150 139 152
158 139 158 152 147 148
152 144 149 148 156 133
148 135 153 151 163 77
Plasmapheresis Group
BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
144 154 159 145 125 157
162 154 173 151 170 173
143 153 161 136 136 179
134 157 173 198 198 173
144 156 145 134 127 145
122 140 171 157 186 171
138 156 150 151 11 174
141 150 144 151 109 157
159 150 148 142 111 168
170 104 155 143 114 194
136 150 157 110 73 135
Group
160 161 162 163 164 165
PRINTANIER DAOUDIYA CAMELIA DOUALIYA
D2 D3 D4 S9 S10 S11
*Reference range: 100 – 270 103/µL [5].
Table 11 White blood cell count (103/µl)* changes in jumping horses subsequent to exercise and a plasmapheresis session.
Group
Control Group
Plasmapheresis Group
Horse
D-2 S1 S2
Time (Days / Samples) D-1 D0 D1 S3 S4 S5 S6 S7 S8
ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
8.7 5.6 10.8 8 7.9 8.1 7.8 11.1 9.3 8.8 10.8 9.4
8.4 6.3 10.6 8.4 8.6 8.4 8.4 10.6 9.4 10.6 10.6 9.7
10.4 8.1 14.2 10.1 10.6 10.1 7.5 15.3 11.7 13.8 14.2 11.7
9.9 7.7 13.9 12.1 12.6 12.1 7.7 13.4 11.6 13.9 13.9 11.6
9.1 8.2 10.2 8.5 10.2 10 9 11 9.9 10.7 10.4 9.9
9.5 10 13.2 11 10.5 8.9 8.1 16.4 11.8 12.3 14.3 11.8
D2 D3 D4 S9 S10 S11
11.8 8.5 7.9 8.1 8 7.6 5.8 5.7 5.6 5.1 14.5 11.1 10.8 10.7 11 12.4 9.3 8.9 8.6 7.2 7.6 8.4 7.6 7.8 7.9 9.9 8.9 8.6 10.2 5.9 12.6 8.7 7.8 7.6 7.6 16.8 14.5 11.3 10.1 11.3 12 9.4 8.8 8.2 7.8 12.5 10.3 9.8 8.1 5.8 14.6 9 8.6 7.8 6.9 12.4 9.3 9.7 9.3 9
166 167 168 169 170
*Reference range: 5.4 -14.3 103/µL [5]. Table 12 Changes in blood lymphocyte count (103/µl)* in jumping horses subsequent to exercise and a plasmapheresis session.
Group
Control Group
Plasmapheresis Group 171 172 173 174 175
D-2 S1 S2
ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
6.9 5.2 6.9 5.9 5.3 5.8 4 12.5 5.9 6.9 6.4 5.9
6.3 5.6 6.3 5.8 5.4 6.3 6.6 5.9 5.8 6.4 6.4 5.8
8.2 10.6 10.9 10.6 9.3 9.5 7.1 9.9 8.8 9.7 13.1 9.2
8.9 10.3 10.3 11.5 10.3 6.3 7.9 9.7 9 9.6 9.1 10
7.9 6.4 6.8 5.9 7.4 6.2 8.3 6.3 7.3 7.3 7.2 7.9
9.6 9.1 7.2 9.6 9.2 9.4 8.6 6 10.6 9.6 10.6 12
7.3 8.3 10.1 9.8 8.6 9.3 8.8 7.2 10.8 10.2 10.9 12.6
7.2 6.2 6.2 7.5 6.5 7.5 7.5 7.9 8.5 8.1 7.2 7
D2 D3 D4 S9 S10 S11 7 5.5 7.5 7.2 6.3 6.2 6.2 7.8 8.1 7.5 6.9 6.9
6.5 5.6 6.4 5.8 5.9 5.5 5.7 6 7.8 7.2 6.8 6.8
6.4 5.7 6.2 5.9 5.5 6.2 5.6 6.5 6 6.8 6.3 5.8
*Reference range: 1.5 – 7.7 103/µl [6]. Table 13 Variations of blood Monocyte count (103/µl)* in jumping horses subsequent to exercise and a plasmapheresis session.
Group
Control Group
Plasmapheresis Group 176 177 178 179 180
Horse
Time (Days / Samples) D-1 D0 D1 S3 S4 S5 S6 S7 S8
Horse
D-2 S1 S2
Time (Days / Samples) D-1 D0 D1 S3 S4 S5 S6 S7 S8
ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
0.2 0.3 0.3 0.2 0.3 0.2 0.2 0.1 0.3 0.2 0.3 0.2
0.2 0.2 0.3 0.3 0.3 0.3 0.1 0.1 0.2 0.2 0.4 0.2
0.3 0.3 0.3 0.4 0.3 0.4 0.3 0.3 0.2 0.4 0.3 0.2
0.2 0.3 0.4 0.5 0.4 0.5 0.3 0.3 0.2 0.3 0.3 0.4
0.2 0.2 0.3 0.2 0.3 0.2 0.2 0.2 0.3 0.3 0.2 0.2
0.3 0.3 0.5 0.4 0.4 0.5 0.4 0.6 0.3 0.3 0.4 0.3
0.4 0.2 0.3 0.2 0.3 0.2 0.4 0.5 0.2 0.4 0.6 0.2
0.2 0.2 0.2 0.3 0.2 0.3 0.2 0.4 0.6 0.2 0.1 0.2
D2 D3 D4 S9 S10 S11 0.2 0.1 0.2 0.3 0.2 0.3 0.3 0.3 0.2 0.3 0.2 0.2
0.3 0.3 0.1 0.1 0.2 0.3 0.3 0.1 0.3 0.3 0.2 0.2
*Reference range: 0 - 0.8 103/µl [8]. Table 14 Blood Eosinophil count (103/µl)* changes in jumping horses subsequent to exercise and a plasmapheresis session.
0.4 0.1 0.2 0.2 0.3 0.1 0.4 0.4 0.1 0.1 0.2 0.2
Group
Control Group
Plasmapheresis Group 181 182 183 184 185
D-2 S1 S2
ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
0.2 0.3 0.3 0.3 0.3 0.2 0.3 0.1 0.4 0.4 0.3 0.2
0.1 0.4 0.3 0.1 0.3 0.2 0.3 0.2 0.3 0.3 0.3 0.4
0.2 0.2 1.3 0.6 1.3 0.6 0.4 0.9 0.8 1.4 1.3 0.9
0.2 0.4 0.9 0.4 0.9 0.4 0.1 0.7 0.9 0.9 0.9 0.9
0.1 0.3 0.3 0.3 0.3 0.3 0.3 0.2 0.3 0.3 0.4 0.5
0.6 0.9 0.7 0.9 0.8 0.9 0.6 1.6 0.8 0.8 1 0.8
0.4 0.5 0.3 0.5 0.5 0.6 0.1 1.5 0.9 0.9 0.8 0.9
0.2 0.1 0.3 0.3 0.4 0.2 0.3 0.5 0.4 0.4 0.3 0.4
D2 D3 D4 S9 S10 S11 0.2 0.2 0.3 0.3 0.4 0.2 0.1 0.4 0.1 0.6 0.7 0.4
0.1 0.3 0.2 0.2 0.3 0.2 0 0.3 0.3 0.5 0.2 0.9
0.1 0.3 0.1 0.3 0.2 0.1 0 0.2 0.5 0.3 0.3 0.2
*Reference range: 0 – 1.0 103/µl [6]. Table 15 Blood Granulocyte count (103/µl)* variations in jumping horses subsequent to exercise and a plasmapheresis session.
Horse
D-2 S1 S2
Time (Days / Samples) D-1 D0 D1 S3 S4 S5 S6 S7 S8
Control Group
ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO
5.9 3.2 6.1 3.7 7.9 3.7
6.1 5.3 6.2 3.6 10.6 3.6
5.1 3 3.5 3.5 8.6 6.1
9.6 5.8 8.6 8.5 7.6 3.3
5.4 1.9 6.3 5.3 7.6 5.3
5.7 2.6 6.4 6.8 12.6 6.8
6.1 3 6.4 5.2 8.4 4.9
5.4 2.6 6.4 4.8 7.8 3.8
5.5 2.9 6.2 4.9 6.5 4.5
5 0.5 5.7 3.6 10.5 2.1
5.3 2.6 6.3 3.4 7.9 2
Plasmapheresis Group
BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
2.8 2.8 4.6 5.5 6.2 4.6
5.2 7.4 5.2 6.3 7.5 6.5
5.2 2.5 5.7 5.1 6.5 5.7
6.3 6.5 6.3 6.4 7.2 6.8
6.3 3.4 5.1 6.3 3.4 5.1
6.1 7 6.5 6.5 7.3 6.2
6.5 7.3 6.3 6.5 7.5 6.4
6.3 3.5 5.1 6.6 4.7 5
4.6 3.4 4.8 5.4 4.3 5.1
4.3 3.5 4.2 4.3 4 5
5.1 3.2 4.1 2.8 3.2 4.6
Group
186 187 188 189 190 191
Horse
Time (Days / Samples) D-1 D0 D1 S3 S4 S5 S6 S7 S8
D2 D3 D4 S9 S10 S11
*Reference range: 2.3 – 8.6 103/µl [7]. Table 16 Variations of glucose (mmol/L) concentrations subsequent to exercise and a plasmapheresis session. Blood glucose concentration (mmol/L)* in jumping horses submitted to plasmapheresis after graded exercise.
Group Control Group
Horse
D-2 S1 S2
Time (Days / Samples) D-1 D0 D1 S3 S4 S5 S6 S7 S8
ACOLITO
5.2
4.5
6
6
4.5
5.6
5.7
5.7
D2 D3 D4 S9 S10 S11 5.6
5.5
5.6
Plasmapheresis Group 192 193 194 195 196 197
7.2 5.5 4.6 5.5 4.6
6 7.1 5.6 7.1 5.6
4.9 6.2 4.5 6.2 4.5
5.8 5.8 5.7 8.2 5.7
4.8 5.3 5.3 6.6 5.3
6.8 6.9 6.3 6.2 6.3
5.3 6.2 5.7 5.8 5.1
5.7 5.8 5.4 5.6 4.8
5.4 5.3 5.3 5.3 5.3
5.3 5.6 4.9 5.6 4.9
5.2 5.9 4.8 6.2 5.4
BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
4.8 5.6 3.8 5.5 7.8 5.8
5.6 5.8 6.5 7.1 5.3 7.1
5.3 4.9 3.6 6 5.5 5.9
6.3 6.6 4.5 6.3 5.6 8.2
5.4 4.9 4.5 6.1 5.4 5.6
5.2 9.3 6.4 6.3 5.4 5.8
5.4 5.5 5.7 5.5 3.6 5.9
5.2 5.5 4.8 6.8 4.8 5
5.1 5.4 4.7 6.7 4.7 4.7
4.9 4.9 4.6 5.9 4.8 4.6
4.6 4.6 4.6 5.6 4.7 5.5
*Reference range: 3.5 – 6.0 mmol/L [9].
Table 17 Modifications of blood urea concentrations (mmol/L)* in jumping horses subsequent to exercise and a plasmapheresis session.
Horse
D-2 S1 S2
Time (Days / Samples) D-1 D0 D1 S3 S4 S5 S6 S7 S8
Control Group
ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO
4.1 4.8 5.3 5 3.9 5
4.2 5.3 5.9 5.4 4.1 5.4
4.1 4.9 5.6 5.2 4 5.1
4.1 5.4 6.3 5.8 4.2 5.8
3.8 5.1 6 5.3 4.5 5
3.9 5.5 6.2 5.6 5 5.7
3.9 5.5 5.8 5.3 4 4.9
3.8 5.6 5.6 5.4 3.9 4.9
3.9 5.1 5.3 5.6 3.8 4.8
3.8 4.9 4.6 4.9 3.7 4.7
3.6 4.7 4.7 4.6 3.5 4.6
Plasmapheresis Group
BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
4.3 3.9 4.5 3.8 6 5.1
4.9 3.9 4.6 4.1 6.5 5.2
4.6 4 4.8 3.9 6 5.1
4.7 4.3 5 4.2 6.5 5.2
4 3.8 5.1 4.6 6.3 4.9
4.2 3.9 5.8 5.3 7.8 4.6
3.6 3.2 4.9 5 6.3 4.3
3.2 3.5 4 5.1 5.6 4.2
3.8 3.2 4.1 5.3 5.4 4
3.7 3 4.1 4.2 5.6 4.1
3.2 3.1 4 3.1 5.6 4
Group
198 199 200 201 202
DAHBI CHAMAA DAHMAN DAMAA DALILANO
D2 D3 D4 S9 S10 S11
*Reference range: 1.2 à 3.6 mmol/L [9]. Table 18 Changes in blood creatinine concentrations (mg/dL)* in jumping horses subsequent to exercise and a plasmapheresis session.
Group
Control Group
Horse
D-2 S1 S2
Time (Days / Samples) D-1 D0 D1 S3 S4 S5 S6 S7 S8
ACOLITO DAHBI CHAMAA DAHMAN DAMAA
1.9 1.7 1.2 1.8 1.4
1.9 1.8 1.2 1.9 1.4
2.2 1.9 1.7 2.5 1.7
2 2.1 1.9 2.4 1.9
2 2 1.3 1.8 1.5
2.4 2.3 1.8 1.9 2
2 2.1 1.3 2 2.2
1.8 2 1.8 1.6 1.8
D2 D3 D4 S9 S10 S11 1.9 2.3 1.7 1.7 1.7
2 2.1 1.5 1.8 1.5
2.1 2 1.4 1.6 1.6
Plasmapheresis Group 203 204 205 206 207
1.8
2.5
1.9
2.4
2.1
2.2
2.1
1.8
1.7
1.8
1.5
BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
1.6 1.5 1.7 1.3 1.4 1.6
2.3 1.5 2.3 1.7 1.6 1.7
1.7 1.5 1.5 1.3 1.7 1.5
2 1.8 2.3 1.9 1.9 1.9
1.6 1.6 1.8 1.4 1.5 1.5
1.9 2 2.2 2.3 1.5 1.5
1.8 1.7 1.8 1.9 1.4 1.4
1.8 1.6 1.7 1.6 1.5 1.4
1.7 1.6 1.8 1.7 1.3 1.8
1.8 1.5 1.7 1.6 1.4 1.7
1.8 1.4 1.8 1.6 1.3 1.5
*Reference range: 0.8 – 1.5 mg/dL [10]. Table 19 Blood Alkaline phosphatase concentrations (U/L)* in jumping horses subsequent to exercise and a plasmapheresis session.
Horse
D-2 S1 S2
Time (Days / Samples) D-1 D0 D1 S3 S4 S5 S6 S7 S8
Control Group
ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO
158 84 117 110 117 110
162 98 141 124 141 112
141 88 127 141 127 112
184 88 144 142 144 142
145 86 140 140 140 111
174 112 154 142 136 125
174 102 150 130 143 124
159 96 132 129 141 124
158 95 139 123 139 123
159 93 140 110 140 110
160 93 141 101 122 118
Plasmapheresis Group
BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
100 131 110 117 161 116
104 139 113 141 139 141
140 88 111 127 160 127
156 156 117 144 142 144
135 136 115 140 159 140
140 177 119 147 140 126
164 141 117 123 148 133
137 141 112 111 171 123
136 132 111 111 165 111
132 129 112 110 169 112
129 121 110 110 146 110
Group
208 209 210 211 212 213
DALILANO
D2 D3 D4 S9 S10 S11
*Reference value : < 250U/L [5].
Table 20 Blood Gama Glutamyl Transferase concentrations (U/L)* in jumping horses subsequent to exercise and a plasmapheresis session.
Group
Control Group
Horse
D-2 S1 S2
Time (Days / Samples) D-1 D0 D1 S3 S4 S5 S6 S7 S8
ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO
10 10 11 10 11 10
10 10 14 10 14 10
10 10 15 10 15 10
10 10 18 10 18 10
10 10 18 10 18 10
10 10 22 10 11 13
10 10 17 10 10 10
10 10 16 10 10 10
10 10 10 10 10 10
10 10 12 10 12 10
10 10 13 10 10 10
BELUC
10 10 10
10 10 10
10 10 11
10 10 12
10 10 10
10 15 13
10 10 10
10 13 10
10 12 10
10 12 10
10 12 10
Plasmapheresis CHADDY Group PRINTANIER
D2 D3 D4 S9 S10 S11
DAOUDIYA CAMELIA DOUALIYA
214 215 216 217 218
15 13 10
18 11 18
18 12 18
13 10 11
14 10 11
11 10 10
11 11 10
10 11 10
10 10 10
Table 21 Blood Total bilirubin concentrations (mg/dL)* in jumping horses subsequent to exercise and a plasmapheresis session.
Horse
D-2 S1 S2
Time (Days / Samples) D-1 D0 D1 S3 S4 S5 S6 S7 S8
Control Group
ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO
2.2 1.7 1.2 1.6 1.2 1.6
2.5 3.8 1.7 1.8 1.7 1.8
1.9 1.6 1.2 1.9 1.2 1.9
2.5 1.7 1.5 2.6 1.5 2.6
2.4 1.6 1.2 2.2 1.4 1.6
2.3 1.6 1.2 3.2 1.2 3.2
2.3 2.3 1.3 1.8 2.6 1.9
2.2 2.1 1.6 1.9 2.3 1.2
2.2 2 1.7 1.5 1.7 1.5
2.4 1.8 1.5 1.6 1.5 1.6
2.3 1.3 1.4 1.4 1.7 1.2
Plasmapheresis Group
BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
3.1 1.7 0.7 1.2 1.6 1.6
1.9 1.9 2.1 2 2.3 2.45
3.2 1.6 1.9 1.2 1.4 1.5
3.7 1.8 2 1.5 1.6 1.5
3.5 1.6 2.1 1.2 1.5 1.2
3.2 2 2.1 1.7 1.7 1.6
3.1 2.1 2.2 1.8 1.6 1
2.6 1.6 1.7 1.7 1.6 1.7
2.1 1.7 1.6 1.6 1.6 1.7
2.3 1.8 1.5 1.3 1.5 1.7
1.5 1.6 0.8 1.1 1.8 1.6
D2 D3 D4 S9 S10 S11
*Reference value: 0.5 – 2.1 mg/dl [10]. Table 22 Blood Sodium concentrations (mmol/L)* in jumping horses subsequent to exercise and a plasmapheresis session.
Horse
D-2 S1 S2
Time (Days / Samples) D-1 D0 D1 S3 S4 S5 S6 S7 S8
Control Group
ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO
138 146 138 138 138 138
143 139 146 143 146 151
138 139 142 138 142 138
144 139 153 142 153 142
143 138 142 139 142 136
143 144 146 149 149 140
138 140 140 139 138 139
138 138 138 138 146 139
142 143 142 129 142 136
143 145 146 121 146 138
144 144 144 123 142 140
Plasmapheresis Group
BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
136 143 145 138 142 148
146 142 149 145 145 146
146 139 144 142 140 141
146 144 145 146 146 146
142 139 146 145 139 146
147 146 145 148 145 144
148 150 149 148 147 147
144 146 145 147 146 146
141 143 145 142 143 145
139 139 149 139 140 149
133 137 150 138 148 141
Group
224 225
14 12 10
*Reference value : < 30 U/l [5].
Group
219 220 221 222 223
11 10 11
*Reference range: 132-142 mmol/L [11, 12].
D2 D3 D4 S9 S10 S11
226 227 228
Table 23 Blood Potassium concentrations (mmol/L) * in jumping horses submitted to plasmapheresis after graded exercise.
Horse
D-2 S1 S2
Time (Days / Samples) D-1 D0 D1 S3 S4 S5 S6 S7 S8
Control Group
ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO
4.4 4.4 3.6 4.2 3.6 4.2
4.6 4.7 4.3 4.6 4.5 4.6
4.3 4.4 4.1 4.5 4.1 4.5
4.7 4.5 4.2 4.6 4.2 4.6
4.2 4.1 4.4 4.7 4.4 4.7
4.7 4.3 4.8 4.9 5.2 5.1
4.6 4.1 4.3 4.5 5.1 5
4.5 4.3 3.9 3.5 4.6 4.8
4.7 4.4 3 3.5 4.6 3
3.5 4.5 3.2 3.8 4.5 3.2
3.1 4.8 3.3 3.9 3.1 3
Plasmapheresis Group
BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
4.4 3.9 4.2 3.8 4 4.2
4.7 4.3 4.8 4.6 4.6 4.5
4.7 4.2 5.3 4.2 3.6 4.2
4.6 4.1 5.1 4.6 4.3 4.2
4.2 3.9 4.8 4.4 4.2 4.4
4.9 4.8 5.2 5.5 3.5 4.4
4.1 4.1 4.1 4.2 3.6 4.1
4.9 4.3 3.1 3.4 4.7 3.1
4.3 4.2 3.6 3.3 3.6 3.3
4.2 4.4 3.2 3.3 3.6 3.2
4.2 4.5 2.7 2.2 3.8 4.1
Group
229 230 231 232 233 234
*Reference range: 3.8 - 5.2 mmol/L [9].
Table 24 Blood Calcium concentrations (mmol/L) (mg/dL)* in jumping horses subsequent to exercise and a plasmapheresis session. Time (Days / Samples) D-1 D0 D1 S3 S4 S5 S6 S7 S8
Horse
D-2 S1 S2
Control Group
ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO
11 12 11.6 11.9 12 11 11.3 12 11.6 11.3 12.6 11.2 11.9 12.3 11.6 11.3 11.5 11
12 11.9 12.1 11.6 12.1 11.4
11.3 11.3 11.2 11.4 11.3 11.4
12.2 11 11.8 12.3 12.2 11.7
11.9 11.6 10.9 11.6 11.9 11.3
11.3 11.9 11.6 11.1 11.8 11.6
11.8 11.6 11.5 11.8 11.5 11.8
11.9 11.7 11.9 11.3 11.9 11.3
11.9 11.8 12 11.3 11.2 11.4
Plasmapheresis Group
BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
11.7 12 11.6 11.3 11.6 11.3 11 12.2 11 11.5 12 11.2 10.5 11.3 10.8 11.3 12 11.2
12 12 12.3 12.1 12.3 12.3
11.6 11.5 11.4 11.3 11.1 11.3
11.9 12.3 11.9 12.3 12 12.3
11.5 10.4 11.3 11.4 10.9 10.3
11.5 10.6 11.8 11.9 10.7 11.6
11.5 10.5 11.8 11.6 10.8 11
11.6 11.2 10.6 11.5 10.6 11.8
11.7 11.3 10.8 11.2 11.2 11.2
Group
235 236 237 238 239 240
D2 D3 D4 S9 S10 S11
D2 D3 D4 S9 S10 S11
*Reference range: 10.8-12.9 mg/dL [10].
Table 25 Plasma albumin (g/L)* changes in jumping horses subsequent to exercise and a plasmapheresis session.
Time (Days / Samples) Horse
D-2 S1 S2
D-1 S3 S4
Control Group
ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO
34 36 35 32 35 33
37 41 39 39 39 39
32 34 36 32 36 32
37 37 37 38 37 38
Plasmapheresis Group
BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
34 36 31 35 36 31
38 38 34 39 32 39
35 34 32 36 37 29
38 39 35 37 30 37
Group
241 242 243 244 245
33 36 38 33 38 33
36 38 38 39 38 38
35 35 32 38 35 38
36 42 36 39 31 33
S7
D1 S8
D2 D3 D4 S9 S10 S11
36 38 38 37 35 36
33 35 38 36 37 32
34 33 33 35 36 35
35 33 35 37 36 36
33 34 37 29 35 30
30 32 26 28 26 25
34 36 32 35 34 31
35 36 31 34 35 31
36 38 32 33 32 32
34 33 32 34 32 32
*Reference range: 26-37g/L [5]. Table 26 Plasma globulin (g/L)* changes in jumping horses subsequent to exercise and a plasmapheresis session.
Horse
D-2 S1 S2
Time (Days / Samples) D-1 D0 D1 S3 S4 S5 S6 S7 S8
Control Group
ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO
33 31 34 28 34 28
36 38 37 37 37 37
31 29 33 31 33 31
35 30 35 35 35 35
31 31 33 34 33 34
36 35 38 36 38 38
33 31 39 38 39 32
32 31 34 35 33 33
33 32 33 34 32 32
34 34 32 36 33 32
35 32 33 37 32 33
Plasmapheresis Group
BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
34 25 30 34 30 34
35 42 33 37 32 37
34 29 33 33 32 36
38 46 35 35 31 35
33 36 30 33 39 33
30 47 35 38 32 41
28 23 26 32 27 32
30 28 30 30 39 34
33 29 34 29 34 29
36 26 33 27 33 27
31 29 34 26 35 28
Group
246 247 248 249 250
S5
D0 S6
D2 D3 D4 S9 S10 S11
*Reference range: 25–45 g/L [5]. Table 27 Changes in Plasma total protein concentrations (g/L)* in jumping horses subsequent to exercise and a plasmapheresis session.
Group
Horse
Control Group ACOLITO
D-2 S1 S2
Time (Days / Samples) D-1 D0 D1 S3 S4 S5 S6 S7 S8
D2 D3 D4 S9 S10 S11
68
63
63
73
72
64
72
70
67
62
63
Plasmapheresis Group 251 252 253 254 255 256
74 72 64 70 66
79 76 76 76 76
73 71 65 74 66
67 76 78 76 78
73 71 65 70 65
73 76 75 76 74
72 76 72 70 70
71 73 66 73 67
71 73 67 74 62
71 69 68 73 64
71 70 68 73 63
BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
68 80 61 69 75 65
73 80 67 76 62 76
69 63 64 74 76 65
76 85 68 76 61 76
68 65 62 71 74 71
66 89 71 77 63 74
66 64 70 68 64 63
67 66 72 70 73 66
67 68 68 72 68 68
65 69 70 68 74 68
69 65 74 69 73 68
*Reference range: 62.5 – 75.0 g/L [13].
Table 28 Changes in blood aspartate Amino-transferase concentrations (U/L)* in jumping horses subsequent to exercise and a plasmapheresis session.
Horse
D-2 S1 S2
D-1 S3 S4
Time D0 S5 S6 S7
Control Group
ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO
242 311 310 192 310 192
275 350 365 251 365 251
276 293 352 254 375 259
285 331 388 263 388 263
283 350 365 255 345 350
304 359 391 267 352 362
302 354 380 282 370 378
287 338 378 256 369 380
279 336 344 246 355 360
275 334 345 232 345 346
274 332 348 226 334 325
Plasmapheresis Group
BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
165 231 300 310 357 335
348 245 310 365 319 365
212 275 325 372 359 375
215 289 349 388 312 388
219 296 358 376 345 389
223 309 359 387 355 400
230 221 295 289 201 230
219 227 315 299 221 238
230 233 321 304 232 256
223 236 305 301 226 306
240 220 318 302 210 235
Group
257 258 259 260 261
DAHBI CHAMAA DAHMAN DAMAA DALILANO
D1 S8
D2 D3 D4 S9 S10 S11
*Reference range: 160-412 U/L [11, 12]. Table 29 Variations of blood creatine kinase concentrations (U/L)* in jumping horses subsequent to exercise and a plasmapheresis session.
Group
Control Group
Horse
D-2 S1 S2
Time (Days / Samples) D-1 D0 D1 S3 S4 S5 S6 S7 S8
ACOLITO DAHBI CHAMAA DAHMAN DAMAA
165 150 149 112 149
172 151 156 212 156
180 160 230 210 169
182 168 268 250 162
136 152 179 172 179
135 175 231 205 201
274 165 133 189 200
212 166 154 212 189
D2 D3 D4 S9 S10 S11 182 165 150 201 169
174 165 136 196 156
169 153 152 189 146
Plasmapheresis Group 262 263 264 265 266
123
215
212
262
172
250
212
236
226
212
189
BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
120 122 123 123 306 149
160 201 151 146 315 212
125 191 128 156 302 146
189 203 159 156 332 232
153 135 132 179 151 179
159 183 149 196 220 202
105 109 110 123 132 132
111 102 103 102 103 123
110 112 114 123 114 112
110 120 117 120 116 115
112 121 120 120 123 123
*Reference range: 60-330 U/L [11 , 12]. Table 30 Changes in blood lactate concentrations (mmol/L)* in jumping horses subsequent to exercise and a plasmapheresis session.
Horse
D-2 S1 S2
Time (Days / Samples) D-1 D0 D1 S3 S4 S5 S6 S7 S8
Control Group
ACOLITO DAHBI CHAMAA DAHMAN DAMAA DALILANO
1 1.2 1.1 1 1.1 1
13.7 15.2 15.4 14.8 18.2 18.6
1.1 1.2 1 1 1.1 1.1
13.5 14.2 16.9 14.6 19.6 20.1
1 1.3 1 1 1.2 1.1
11.4 12.6 17.8 14.7 20.9 19.7
2.4 2.2 3.4 3.1 4.1 3.9
1 1.2 1.1 1.1 1.2 1.1
1 1.3 1.1 1 1.1 1.1
1 1.2 1 1 1.1 1
1 1.3 1.1 1.1 1.1 1.1
Plasmapheresis Group
BELUC CHADDY PRINTANIER DAOUDIYA CAMELIA DOUALIYA
1 1.1 1 1 1.2 1.2
13.2 13.5 23.4 14.7 19.6 17.9
1 1 1.3 1 1.1 1.1
13.3 16 21.9 14.9 20.8 18.1
1 1 1.2 1 1 1.2
14.5 14.3 24.5 15.1 21.1 18.4
1.1 1.2 1.3 1.1 1.1 1.1
1 1.1 1.1 1 1.1 1
0.9 1.1 1.1 0.8 1 0.9
0.9 1.1 0.9 0.8 1 0.8
0.9 1 0.9 0.9 1 0.9
Group
267 268 269 270 271
DALILANO
D2 D3 D4 S9 S10 S11
*Reference value : < 2.5 mmol/L [9]. 2. Experimental Design, Materials, and Methods Investigations were carried out on twelve show jumping horses, they randomly allocated to two
272
groups: a plasmapheresis group (n=6) and a control group (n=6). All horses were healthy and
273
received the same food diet three times a day. First in the morning, they were offered each 1kg
274
of commercial feed (Destrier, France). Then, in midday, they received each 2.5kg of barley and
275
its bran (0.5kg) in addition to 30g of a vitamin and mineral supplement. Finally, in the evening,
276
horses were fed each 0.5kg of oats and 2kg of barley. A supplement of 2.5kg of alfalfa hay was
277
given for each horse after each food ration distribution. Water was provided ad-libitum.
278
Horses of both groups underwent each morning an exercise of 30 min/day, during 3 successive
279
days (Day -2, -1 and 0). Blood was sampled before and after exercise. Following the 3rd day
280
exercise (Day 0) and its corresponding subsequent blood sampling, a plasmapheresis session
281
was performed on the horses of the plasmapheresis group while horses of the control group
282
were maintained in the same experimental environment but without being subjected to
283
plasmapheresis. Then blood was sampled from each horse of both groups. Subsequent daily
284
blood samples were taken at the same hour for the following 4 days (Day 1, 2, 3 and 4). All
285
blood samples were obtained using jugular venipuncture. Sampling was realized in the morning
286
between the two first food diets of the day.
287
In order to verify the effect of plasmapheresis, the physical exercise executed by horses
288
subjected to horse should be capable to have to comply the condition of inducing a significant
289
raise of several hematobiochemical parameters and specifically providing moderate to high
290
values of lactates. The well-known triangular exercise carried out in the trotter horses by
291
Demonceau and Auvinet [1] [2] have been first used. This exercise corresponds to succession
292
of warm-up period (15 minutes) and 11minutes of workloads exercise (steps) and then a
293
recovery period of 10 minutes. Data of lactate concentrations obtained from blood samples
294
taken 5 to 10 minutes after exercise test finished varied from 2.0 and 3.7 mmol/L (Figure 1B).
295
To obtain high values of lactates, the previous exercise test was modified by increasing the
296
duration of the warm-up phase up to 30min and steps were substituted by gallop. The new
297
warm-up stage consisted on 5min trot, 3min of gallop, 5min of trot, 3min of gallop, 5min trot,
298
3min of gallop and finally 6min of trot. The speeds of horses at trot was 240 m/min while it
299
was 350 m/min for gallop. Data of blood lactate concentrations increased by 6 times (Figure
300
1B) varying between 13.2 and 23.4mmol/L. This new exercise was then used in the following
301
experiment investigating the effect of plasmapheresis.
302
303
Figure 1. Lactate concentrations in six jumping horses. (A): Before and following the
304
exercise test standardized by Demonceau and Auvinet. (B): Before and following the
305
modification of the warm-up phase of the same exercise test. The duration of this phase was
306
doubled to 30 min instead of 15 min and steps were substituted by gallop.
307 308
Plasmapheresis was performed by harvesting of 7L of plasma per horse which are replaced by
309
7L of NaCl 0.9%. The technique was performed by using a small-sized plasmapheresis
310
machine commercialized by Hemofenix-France utilizing Trackpore technology (Dubna
311
Moscow region-Russian Federation). The machine is equipped with Rosa® type membrane
312
filters with 0.4µm pores. The system is totally automated operating.
313
Haematological parameters included the number of red blood cells (RBCs), hemoglobin
314
concentration and hematocrit, the mean corpuscular volume (MCV), the mean corpuscular
315
hemoglobin (MCH), the mean corpuscular hemoglobin concentration (MCHC), the number of
316
platelets and also the number of white blood cells (WBCs) and the count of lymphocytes,
317
monocytes, eosinophils and granulocytes. All these parameters were determined using a
318
veterinary cell-counter automate, the Celltac VET MEK-6550 hematology (Nihon Kohden,
319
Tomioka-Japan) and its reagents, Hemolynac·3 MEK-660I, Isotonac·4 MEK-641I, Cleanac
320
MEK-520I (Celltac VET MEK-6550 J/K hematology, Nihon Kohden, Tomioka-Japan).
321
Concerning the biochemical parameters, the concentrations of albumin, globulin, total protein,
322
glucose, alkaline phosphatase (ALP), aspartate aminotransferase (AST), gamma glutamyl
323
transferase (GGT), total bilirubin, lactate, creatinine kinase (CK), urea, creatinine, calcium,
324
sodium and potassium were determined using the veterinary Skyla-VB1 automate analyzer
325
(Lite-On Technology Corporation, Hsinchu-Taiwan). All the chemical reactions completed
326
inside a circular and transparent plastic reagent disc (Equine Panel- Product code 900-150,
327
skylaTM VB1 reagent disc, Hsinchu-Taiwan), containing different reaction cuvettes with
328
specific assay reagent for each measured parameter. Concentrations quantified by photometric
329
measurement of the absorbance changes arising from the chemical reactions in cuvettes’ disc
330
are automatically given by the analyzer.
331
Lactate concentration was assayed using the portable analyzer Lactate-Pro™ 2. This test meter
332
is based on a reaction of the blood lactate with a reagent in the Lactate-Pro™ 2 Test Strip
333
(Busimedic®, S.L, San Sebastián-Spain). To validate and to ensure the accuracy of this
334
technique, blood lactate concentrations measured using the Lactate-Pro™2 were compared to
335
those measured by a reference analyzer, COBAS™ Gen.2 using the reagent COBAS
336
INTEGRA Lactate Gen.2 (Roche®, Maylan-France). These analyses were conducted on
337
specimens of six Athlete show jumping horses having performed the developed exercise, as
338
previously described. Blood was sampled before and after the exercise and lactate
339
concentration was immediately assayed by Lactate-Pro™2 and COBAS™ Gen.2 analyzers.
340
Measurements from both instruments were tested for correlation.
341 342
For further information concerning the experimental procedures please see the research
343
article [2][1]accompanying this data paper.
344 345 346 347
Acknowledgments Authors of this research work express sincere recognition to the Moroccan Royal Guard, the
348
Hassan II Institute of Agronomy and Veterinary Medicine (IAV Hassan II), the Royal Horse
349
Encouragement Society (SOREC), the Society for the Protection of Animals and Nature
350
(SPANA-Morocco), GIGA-LAB Morocco and finally Hemofenix France, funding this
351
research.
352 353 354 355 356 357 358 359 360 361 362 363 364 365 366
References [1] Daden R., Zarhouni F. Z., Chakir J., Piro M., Achaaban M.R., Ouassat M. & El Allali K. Plasmapheresis effect on haematological and biochemical parameters in athletic horses subjected to exercise. https://doi.org/10.1016/j.jevs.2019.07.006. [2] Demonceau T, Auvinet B. Exercise test for trotters in training: practical realization and first results. In: Report of the 18th Study Day. CEREOPA 1992; 120–132. Paris. [3] Marlin D., Nankervis K. (2013). Equine Exercice Physiology. Edition by Blackwell Publishing Company. [4] Huntington.P , Myers.J and Owens.E . (2004). Horse Sense : The Guide to Horse Care in Australia and New Zealand. Second edition , Ch 6 p:176. [5] Orsini A. and Divers J. (2001). Urgences en médecine équine, Traitement et procédures. MALOINE edition. 834p
367 368 369 370 371 372 373 374 375 376 377 378 379 380 381 382 383 384 385 386 387 388 389 390 391 392 393 394 395 396 397 398
[6] Grondin T.M., Dewitt S.F. Normal Hematology of the Horse and Donkey. In: Weiss D.J., Wardrop K.J. (dir.) (2010). Schalm´s Veterinary Hematology. Ames, Iowa, USA: Wiley Blackwell, pp. 821-828. [7] Desjardins I., Cadoré J.L. (2006). Analyses sanguines équines. I – Hématologie: approche Clinique. Pratique Vétérinaire Equine, 38(151), pp. 9-22. [8] Weiser G., Thrall M.A. Introduction to Leukocytes and the Leukogram. In: Thrall M.A., Baker D.C., Campbell T.W., Denicola D., Fettman M.J., Lassen E.D., Rebar A., Weiser G. (dir.) (2004). Veterinary Hematology and Clinical Chemistry. Baltimore, MD: Lippincott Williams & Wilkins, pp. 125-130. [9] Jaillardon. L (2017) . Petit memento de biochimie. Laboratoire ONIRIS. Site de la Chantrerie. Nantes. [10] Cornell University. Cornell University College of Veterinary Medicine [en ligne]. URL: https://ahdc.vet.cornell.edu/sects/clinpath/reference/chem.cfm (viewed on 17th August 2019 ) [11] Rose R. et Hodgson D. (1999). Hematology And Biochemistry. Dans R. H. Rose, The Athletic Horse. (Pp. 63-78). Saunders. [12] Smith BP. (2002). Large Animal Internal Medicine : diseases of horses, cattle, sheep and goats., 3th edition. St Louis : Mosby Cie,:1-1735. [13] Soares, J.C.M., Zanella, R., Bondan, C., Alves, L.P., Ragagnin de Lima, M., Costa da Motta, A., Zanella, E.L. (2011) Biochemical and antioxidant changes in plasma, serum, and erythrocytes of horses before and after a jumping competition. Journal of Equine Veterinary Science, 31, 357-360.
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46