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Decarboxylase activity and zinc
P3-26 DECARBOXYLASE ACTIVITY AND ZINC Y. Shibata, Y.Sotokawa, M. Imai, H. Ishizu, M. Nakatsuka, T.Ohta, Y. Matsuda, F.Takeuchi,R. Tsubouchi,M. Haneda, M. Yoshino Department of Biochemistry, Aichi Medical University, Nagakute, Aichi,Japan
Okinaka and Nakasa r e p o r t e d t h a t Zn 2÷ i n h i b i t e d d e c a r b o x y l a s e a c t i v i t y in v i t r o ~) and a l s o Shibata and Nakatsuka r e p o r t e d t h a t Zn 2÷ c o n t e n t in serum incr eas ed in P a r k i n s o n ' s d i s e a s e s ~ In t h i s paper,we r e p o r t t h a t 1.The a c t i v i t y of b r a i n DOPA d e c a r b o x y l a s e d e c r e a s e d in excess Zn ~÷ a d m i n i s t e r e d rats. 2. This change was e s p e c i a l l y observed in the p a r t of s t r i a t u m of brai n. 3.5-OH-Tryptophan d e c a r b o x y l a s e a c t i v i t y was not so changed in b r a i n . 4. Serum Zn 2+ c o n t e n t i n c r e a s e d . In t h i s r e p o r t , w e i n d i c a t e t h a t the d e c a r b o x y l a s e a c t i v i t y might be a f f e c t e d by Zn z÷ c o n t e n t in b r a i n . R ef er en ces 1)T. Nakasa, J. Aichi Med. Univ. Assoc. Vol.13, No. 3, pp. 419-440(1985) 2)M. Nakatsuka, F. Takeuchi,R. Tsubouchi,M. Haneda, T. Ohta, Y. Shibata, h l z h e i m e r ' s and P a r k i n s o n ' s D i s e a s e s , B a s i c and T h e r a p e u t i c S t r a t e g i e s The Second I n t e r n a t i o n a l Conference, A b s t r a c t , 144(1989)
P3-27 THE EFFECT OF ANABOLIC STEROIDS EXTRACTEDFRON NATURALHERB ON SLOWINGAGING Dons Chess-Tong, Dong Yin, Wang Zhong-Tao, Li Ru-Qing I n s t i t u t e of Basic Medicine, Hebei Medical college, People's Republic of China
The herb extract was analysed by gas chromatography-mass spectromo technique, it contains 40 kinds of anabolic steroids: 5A-androstane-3A, 17B-diol; 5A- androstane 3A, 17B- diol 3acetate; 5A-androstane-17B-OL-3-one benzoate;5-androstene-3B, 17B-diol;5-androstene- 3B, 17Bdiol-3-acetate;5-androstene-3B, 17B-diol 17-benzoate;5-androstene-3B, 17B- diol diacetate; 5androstene-3B, 17B-diol diproplonate;androsterene;androsterone acetate; boldenone; boldenone undeoylenate;danazol;dehydroisoandrosterone;dehydroisoandrosterone 3-acetate;fluoxymesterone; mestanolone; methandriol; methandrlol dipropionate; methandrostenolone methyltestosterone; nandrolone; nandrolone phenylpropionate; nandrolone propionate; oxandrolone; oxymetholone s t a n o z o l o l ; t e s t o s t e r o n e ; t e s t o s t o r o n e a c e t a t e ; t e s t o s t e r o n e benzoate, etc, The e f f e c t s of steroids on rats and mice as following: giving protection against the morphological aging of the brain, liver, heart, t e s t i s , thyroid gland, kindney and adrenal cortex;improving the secretion of prostate and the amount of sperm; promoting the c e l l u l a r immunity and the phagocytes is, and also the humeral immunity; inhibiting the inflammatory reaction and the granuloma; enable quick minrocirculation and re- opening the closed c a p i l l a r i e s , and increasing in the mesenteric lymphatic spontaneous c o n t r a c t i l e frequency ; inhibiting the arthroedema; diminishing the free radical metabolite (MDA)in brain; enhancing the force and endurance of muscular contraction. The e f f i c a n c i e s of the extract in adult and aged people as following: improving sexual function and secondary sexual features and increasing in the secretion of somem, white hairs return to normal colour; hairs regrow on its lost area;skin senilmacula and verrula senili6 disappears; eliminating edema and improving nocturia; releasing from dysmenorrhea; releasing from angina pactorls; eliminating skin wrinkles; promoting the a b i l i t y of adaptation to environment and reeietance to diocese, etc.
495
Okinaka and Nakasa r e p o r t e d t h a t Zn 2÷ i n h i b i t e d d e c a r b o x y l a s e a c t i v i t y in v i t r o ~) and a l s o Shibata and Nakatsuka r e p o r t e d t h a t Zn 2÷ c o n t e n t in serum incr eas ed in P a r k i n s o n ' s d i s e a s e s ~ In t h i s paper,we r e p o r t t h a t 1.The a c t i v i t y of b r a i n DOPA d e c a r b o x y l a s e d e c r e a s e d in excess Zn ~÷ a d m i n i s t e r e d rats. 2. This change was e s p e c i a l l y observed in the p a r t of s t r i a t u m of brai n. 3.5-OH-Tryptophan d e c a r b o x y l a s e a c t i v i t y was not so changed in b r a i n . 4. Serum Zn 2+ c o n t e n t i n c r e a s e d . In t h i s r e p o r t , w e i n d i c a t e t h a t the d e c a r b o x y l a s e a c t i v i t y might be a f f e c t e d by Zn z÷ c o n t e n t in b r a i n . R ef er en ces 1)T. Nakasa, J. Aichi Med. Univ. Assoc. Vol.13, No. 3, pp. 419-440(1985) 2)M. Nakatsuka, F. Takeuchi,R. Tsubouchi,M. Haneda, T. Ohta, Y. Shibata, h l z h e i m e r ' s and P a r k i n s o n ' s D i s e a s e s , B a s i c and T h e r a p e u t i c S t r a t e g i e s The Second I n t e r n a t i o n a l Conference, A b s t r a c t , 144(1989)
P3-27 THE EFFECT OF ANABOLIC STEROIDS EXTRACTEDFRON NATURALHERB ON SLOWINGAGING Dons Chess-Tong, Dong Yin, Wang Zhong-Tao, Li Ru-Qing I n s t i t u t e of Basic Medicine, Hebei Medical college, People's Republic of China
The herb extract was analysed by gas chromatography-mass spectromo technique, it contains 40 kinds of anabolic steroids: 5A-androstane-3A, 17B-diol; 5A- androstane 3A, 17B- diol 3acetate; 5A-androstane-17B-OL-3-one benzoate;5-androstene-3B, 17B-diol;5-androstene- 3B, 17Bdiol-3-acetate;5-androstene-3B, 17B-diol 17-benzoate;5-androstene-3B, 17B- diol diacetate; 5androstene-3B, 17B-diol diproplonate;androsterene;androsterone acetate; boldenone; boldenone undeoylenate;danazol;dehydroisoandrosterone;dehydroisoandrosterone 3-acetate;fluoxymesterone; mestanolone; methandriol; methandrlol dipropionate; methandrostenolone methyltestosterone; nandrolone; nandrolone phenylpropionate; nandrolone propionate; oxandrolone; oxymetholone s t a n o z o l o l ; t e s t o s t e r o n e ; t e s t o s t o r o n e a c e t a t e ; t e s t o s t e r o n e benzoate, etc, The e f f e c t s of steroids on rats and mice as following: giving protection against the morphological aging of the brain, liver, heart, t e s t i s , thyroid gland, kindney and adrenal cortex;improving the secretion of prostate and the amount of sperm; promoting the c e l l u l a r immunity and the phagocytes is, and also the humeral immunity; inhibiting the inflammatory reaction and the granuloma; enable quick minrocirculation and re- opening the closed c a p i l l a r i e s , and increasing in the mesenteric lymphatic spontaneous c o n t r a c t i l e frequency ; inhibiting the arthroedema; diminishing the free radical metabolite (MDA)in brain; enhancing the force and endurance of muscular contraction. The e f f i c a n c i e s of the extract in adult and aged people as following: improving sexual function and secondary sexual features and increasing in the secretion of somem, white hairs return to normal colour; hairs regrow on its lost area;skin senilmacula and verrula senili6 disappears; eliminating edema and improving nocturia; releasing from dysmenorrhea; releasing from angina pactorls; eliminating skin wrinkles; promoting the a b i l i t y of adaptation to environment and reeietance to diocese, etc.
495