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Decidua-based γδ T cells are enriched and fully differentiated during early human pregnancy
Abstracts / Journal of Reproductive Immunology 122 (2017) 37–50
Conclusion: Testing of plasma sHLA-G in patients who undergo IVF-ET could be useful and may have prognostic value. This study was funded by the Polish National Science Centre (no. 2014/13/B/NZ5/00273).
P9
http://dx.doi.org/10.1016/j.jri.2017.07.028
Shuyi Yu, Yuye Li, Chunyu Huang, Chenyang Du, Cong Chen, Lanna Chen, Yong Zeng ∗
P8 Decidua-based ␥␦ T cells are enriched and fully differentiated during early human pregnancy A. Terzieva 1,∗ , Y. Hristova 2 , V. Dimitrova 2 , P. Dimitrova 3 , T. Dimova 1 1 Institute of Biology and Immunology of Reproduction “Acad. K. Bratanov”, Bulgarian Academy of Sciences, Sofia, Bulgaria 2 Medical University, University Obstetrics and Gynecology Hospital “Maichin Dom”, Sofia, Bulgaria 3 Institute of Microbiology “Acad. St. Angeloff”, Bulgarian Academy of Sciences, Sofia, Bulgaria
Background: Numerous studies have shown the important role of ␥␦ T cells as a link between innate and adaptive immune systems in the protection against infections, in tumor surveillance and in tissue repair in both inflammatory and metabolic stress. Maternal immune system during pregnancy is provoked by metabolic stress associated with vascular remodeling and placenta formation as well as by adoption of fetus with foreign genetic code. Data about importance of ␥␦ T cells for implantation/pregnancy outcome are still conflicting and further research needs to be carried out. We aimed to investigate early pregnancy induced changes in the amount and phenotype of decidual and peripheral maternal ␥␦ T cells. Material and methods: Decidual and blood samples from healthy women in early pregnancy and from non-pregnant women of reproductive age were examined by immunohistochemistry and by ex vivo flow cytometry. Results: ␥␦ T cells are resident cells of decidual tissue, distributed either in clusters adjacent to the uterine glands or scattered as single cells. Their analysis during early pregnancy revealed three important findings: (1) the amount of ␥␦ T cells at the maternal–fetal interface was higher than in the blood; (2) early pregnancy did not affect the amount of peripheral blood ␥␦ T cells; (3) terminally differentiated ␥␦ T cells predominated in the decidua, but not in the blood of pregnant women. Conclusion: Early pregnancy triggers an influx of terminally differentiated ␥␦ T cells at the materno-fetal interface. This study is funded by Bulgarian National Science Fund, project DH 03/5. http://dx.doi.org/10.1016/j.jri.2017.07.029
47
Increased CD8+ CD57+ cells are associated with recurrent miscarriage and repeated implantation failure
Shenzhen Key Laboratory of Reproductive Immunology for Peri-Implantation, Shenzhen Zhongshan Institute for Reproduction and Genetics Fertility Center, Shenzhen Zhongshan Urology Hospital, Shenzhen, Guangdong, PR China Background: Dysfunction of maternal immune cells can induce reproductive failure, but little work has been focused on the function of CD8+ cell on maternal–fetal interface. Previous studies had proved that CD57 expression correlated strongly with simultaneous expression of gA, gB, and Perf that could mark high cytolytic potential (P.K. Chattopadhyay, 2008, J. Leukocyte Biol.). However, the expression levels of CD57 in CD8+ T cells are unknown. Material and methods: Double immunohistochemical staining was performed on a BOND III Immunostainer (Leica Microsystems). CD57 was visualized with DAB chromogen and CD8 was visualized with Fast Red chromogen in endometrium taken from the women with RM (n = 8), RIF(n = 8) and controls (n = 5) during mid-luteal period. The images were acquired and analyzed by the ® Vectra automated quantitative pathology imaging system. Differences in the immune cells between two groups were determined by using independent t-test (the normally distributed data) or Mann–Whitney U test (the abnormally distributed data) as indicated. Results: Compared to control group, the number of CD8+ cells, CD57+ cells and CD8+ CD57+ cells were significantly increased in RM (P < 0.05, P < 0.001, P < 0.01) and RIF (P < 0.05, P < 0.01, P < 0.05) group (Fig. 1).
Fig. 1. Double immunohistochemical staining of CD8 (brown) and CD57 (fast red) positive endometrial cells in control, RIF and RM women.
Conclusion: We found CD8+ CD57+ cells were significantly increased in RM and RIF patients, suggesting CD8+ cell with high cytolytic ability may be involved in reproductive failure. http://dx.doi.org/10.1016/j.jri.2017.07.030
Conclusion: Testing of plasma sHLA-G in patients who undergo IVF-ET could be useful and may have prognostic value. This study was funded by the Polish National Science Centre (no. 2014/13/B/NZ5/00273).
P9
http://dx.doi.org/10.1016/j.jri.2017.07.028
Shuyi Yu, Yuye Li, Chunyu Huang, Chenyang Du, Cong Chen, Lanna Chen, Yong Zeng ∗
P8 Decidua-based ␥␦ T cells are enriched and fully differentiated during early human pregnancy A. Terzieva 1,∗ , Y. Hristova 2 , V. Dimitrova 2 , P. Dimitrova 3 , T. Dimova 1 1 Institute of Biology and Immunology of Reproduction “Acad. K. Bratanov”, Bulgarian Academy of Sciences, Sofia, Bulgaria 2 Medical University, University Obstetrics and Gynecology Hospital “Maichin Dom”, Sofia, Bulgaria 3 Institute of Microbiology “Acad. St. Angeloff”, Bulgarian Academy of Sciences, Sofia, Bulgaria
Background: Numerous studies have shown the important role of ␥␦ T cells as a link between innate and adaptive immune systems in the protection against infections, in tumor surveillance and in tissue repair in both inflammatory and metabolic stress. Maternal immune system during pregnancy is provoked by metabolic stress associated with vascular remodeling and placenta formation as well as by adoption of fetus with foreign genetic code. Data about importance of ␥␦ T cells for implantation/pregnancy outcome are still conflicting and further research needs to be carried out. We aimed to investigate early pregnancy induced changes in the amount and phenotype of decidual and peripheral maternal ␥␦ T cells. Material and methods: Decidual and blood samples from healthy women in early pregnancy and from non-pregnant women of reproductive age were examined by immunohistochemistry and by ex vivo flow cytometry. Results: ␥␦ T cells are resident cells of decidual tissue, distributed either in clusters adjacent to the uterine glands or scattered as single cells. Their analysis during early pregnancy revealed three important findings: (1) the amount of ␥␦ T cells at the maternal–fetal interface was higher than in the blood; (2) early pregnancy did not affect the amount of peripheral blood ␥␦ T cells; (3) terminally differentiated ␥␦ T cells predominated in the decidua, but not in the blood of pregnant women. Conclusion: Early pregnancy triggers an influx of terminally differentiated ␥␦ T cells at the materno-fetal interface. This study is funded by Bulgarian National Science Fund, project DH 03/5. http://dx.doi.org/10.1016/j.jri.2017.07.029
47
Increased CD8+ CD57+ cells are associated with recurrent miscarriage and repeated implantation failure
Shenzhen Key Laboratory of Reproductive Immunology for Peri-Implantation, Shenzhen Zhongshan Institute for Reproduction and Genetics Fertility Center, Shenzhen Zhongshan Urology Hospital, Shenzhen, Guangdong, PR China Background: Dysfunction of maternal immune cells can induce reproductive failure, but little work has been focused on the function of CD8+ cell on maternal–fetal interface. Previous studies had proved that CD57 expression correlated strongly with simultaneous expression of gA, gB, and Perf that could mark high cytolytic potential (P.K. Chattopadhyay, 2008, J. Leukocyte Biol.). However, the expression levels of CD57 in CD8+ T cells are unknown. Material and methods: Double immunohistochemical staining was performed on a BOND III Immunostainer (Leica Microsystems). CD57 was visualized with DAB chromogen and CD8 was visualized with Fast Red chromogen in endometrium taken from the women with RM (n = 8), RIF(n = 8) and controls (n = 5) during mid-luteal period. The images were acquired and analyzed by the ® Vectra automated quantitative pathology imaging system. Differences in the immune cells between two groups were determined by using independent t-test (the normally distributed data) or Mann–Whitney U test (the abnormally distributed data) as indicated. Results: Compared to control group, the number of CD8+ cells, CD57+ cells and CD8+ CD57+ cells were significantly increased in RM (P < 0.05, P < 0.001, P < 0.01) and RIF (P < 0.05, P < 0.01, P < 0.05) group (Fig. 1).
Fig. 1. Double immunohistochemical staining of CD8 (brown) and CD57 (fast red) positive endometrial cells in control, RIF and RM women.
Conclusion: We found CD8+ CD57+ cells were significantly increased in RM and RIF patients, suggesting CD8+ cell with high cytolytic ability may be involved in reproductive failure. http://dx.doi.org/10.1016/j.jri.2017.07.030