- Email: [email protected]
Decursin inhibits tumor necrosis factor-alpha-induced insulin resistance in C2C12 skeletal muscle cells
Abstracts / Journal of Biotechnology 136S (2008) S75–S98
S91
exhibit good inhibition ability in MITF-DNA binding intensity test by MITF protein chip and also, it displayed low toxicity and melanin synthesis inhibition in the bioassay.
I3-P-048
References
Ae-Hee Kim ∗ , Ik-Hwan Kim
Han, J.S., Kwak, E.Y., Lee, H.B., Shin, J.H., Baek, S.H., Chung, B.H., Kim, E.K., 2004. Manufacturing protein-DNA chip for depigmenting agent. J. Soc. Cosmet. Scientists Korea, 479–483. Parvez, S., Kang, M., Chung, H.S., Cho, C., Hong, M.C., Shin, M.K., Bae, H., 2006. Survey and mechanism of skin depigmenting and lightening agents. Phytother. Res. 20, 921–934. Solano, F., Briganti, S., Picardo, M., Ghanem, G., 2006. Hypopigmenting agents: an updated review on biological, chemical and clinical aspects. Pigment Cell Res. 19 (December (6)), 550–571. S.H. Yang, 2007. Development of Protein Chip for HTS System of Depigmenting Agent Screening. Master Thesis. University of Inha, Incheon, Korea.
School of Life Sciences and Biotechnology, Korea University, Anam 5th Street, Seongbuk-Gu, Seoul 136-701, Republic of Korea
doi:10.1016/j.jbiotec.2008.07.205 I3-P-047 Decursin inhibits tumor necrosis factor-alpha-induced insulin resistance in C2C12 skeletal muscle cells Ji-Eun Kang ∗ , Ik-Hwan Kim School of Life Sciences and Biotechnology, Korea University, Anam 5th Street, Seongbuk-Gu, Seoul 136-70, Republic of Korea E-mail address: [email protected] (J.-E. Kang). Insulin resistance is a key feature of type 2 diabetes and is a characteristic of wide range of other clinical tests and experiments (Karim and Juleen, 2007). Systemic inflammation is also a feature of obesity and type 2 diabetes, raising the hypothesis that elevated cytokine levels may contribute to peripheral insulin resistance (Nicholas et al., 2006). In this study, the insulin resistance was established with the treatment of an inflammatory cytokine, tumor necrosis factor-alpha (TNF-alpha) against C2C12 skeletal muscle cells. The TNF-alpha treatment was well-validated experimental models for insulin resistance by impairing insulin signaling events involved in GLUT4 translocation and inducing ROS accumulation. We induced C2C12 differentiation by substituting the medium DMEM supplemented with 2% horse serum for 10% FBS. The development of TNF-alpha-induced insulin resistance will be determined by glucose uptake. Glucose transport is evaluated by measuring [3 H] 2-deoxy-d-glucose and cellular ROS levels are measured by using DCF-DA. Decursin is a compound containing coumarin ring isolated from a root of Angelica gigas (Kim et al., 2005). The effect of decursin sensitizing insulin signaling against the TNF-alpha-induced insulin resistance will be presented. The mechanism of action of decursin against insulin resistance also will be suggested.
Protective effect of decursin against PMA-induced cytotoxicity in lung cell fibroblast
E-mail address: [email protected] (A.-H. Kim). Protein kinase C (PKC) has become an important target for therapeutic intervention in cancer, cardiovascular, neurological, metabolic and immunological disorders and has been considered as a target of anticancer drug screening because it is the cellular receptor of tumor-promoting phorbol esters. Phorbol 12-myristate 13-acetate (PMA), the most commonly used phorbol ester, binds to PKC and activates it (Gelsher and Dale, 1989). The activated PKC causes an extremely wide range of effects in cells and tissues. In many cell types, including fibroblasts and T lymphocytes, phorbol esters stimulate cellular proliferation, suggesting a role for PKC activation in this process (Berry and Nishizuka, 1990; Nomura et al., 2007). Decursin, a PKC activator anticancer agent isolated from Angelica gigas was cytotoxic to cells (Kim et al., 2005; Yim et al., 2005). In this study, the effect of decursin in lung cell fibroblast and the mechanism of action against PMA will be protected. In order to examine the effect of decursin against PMA-induced cytotoxicity in lung cell fibroblast, cell viability, apoptosis, cell cycle and ROS levels were measured by FACS. To analyze signal pathways, western blot and immunocytochemistry would be presented. References Berry, N., Nishizuka, Y., 1990. Protein kinase C and T cell activation. Eur. J. Biochem. 189, 205–214. Gelsher, A., Dale, I.L., 1989. Protein kinase C—a novel target for rational anti-cancer drug design? Anticancer Drug Des. 4, 93–105. Kim, H.H., Bang, S.S., Choi, S.J., Han, H., Kim, I.H., 2005. Involvement of PKC and ROS in the cytotoxic mechanism of anti-leukemic decursin and its derivatives and their structure-activity relationship in human K562 erythroleukemia and U937 myeloleukemia cells. Cancer Lett. 223, 191–201. Nomura, N., Nomura, M., Takahira, M., Sugiyama, K., 2007. Phorbol 12-myristate 13-acetate-activated protein kinase C increased migratory activity of subconjunctival fibroblasts via stress-activated protein kinase pathways. Mol. Vis. 13, 2320–2327. Yim, D.S., Singh, R.P., Agarwal, C., Lee, S.Y., Choi, H.J., Agarwal, R., 2005. A novel anticancer agent, decursin, induces G1 arrest and apoptosis in human prostate carcinoma cells. Cancer Res. 65, 1035–1044.
doi:10.1016/j.jbiotec.2008.07.207 I3-P-051 Styraxjaponoside C mediates its antifungal activity via apoptosis
References
Cana Park ∗ , Woo Sang Sung, Juneyoung Lee, Dong Gun Lee ∗
Karim, B., Juleen, R.Z., 2007. MAP4K4 Gene silencing in human skeletal muscle prevents tumor necrosis factor-induced insulin resistance. J. Biol. Chem. 282, 7783–7789. Kim, H.H., Sik, B.S., Seok, C.J., Han, H., Kim, I.H., 2005. Involvement of PKC and ROS in the cytotoxic mechanism of anti-leukemic decursin and its derivatives and their structure activity relation ship in human K562 erythroleukemia and U937 myeloleukemia cells. Cancer Lett. 223, 191–201. Nicholas, H., Evan, D.R., Eric, S.L., 2006. Reactive oxygen species have a causal role in multiple forms of insulin resistance. Nature 440, 944–994.
School of Life Sciences & Biotechnology, College of Natural Sciences, Kyungpook National University, Daegu, Republic of Korea
doi:10.1016/j.jbiotec.2008.07.206
E-mail address: [email protected] (C. Park). Styraxjaponoside C is a novel glycoside isolated from the stem bark of Styrax japonica S. et Z., which is one of the lignans (Kim et al., 2007). In this study, we investigated novel antimicrobial effects and its mode of action of Styraxjaponoside C. The result showed that Styraxjaponoside C exhibited antimicrobial activity against human pathogenic microorganisms without hemolytic effect on human erythrocytes. To elucidate the antimicrobial effects of Styraxjaponoside C, its effects on the dimorphism of Candida albicans induced by fetal bovine serum (FBS), which plays a key role in the pathogenesis of a host invasion, were
S91
exhibit good inhibition ability in MITF-DNA binding intensity test by MITF protein chip and also, it displayed low toxicity and melanin synthesis inhibition in the bioassay.
I3-P-048
References
Ae-Hee Kim ∗ , Ik-Hwan Kim
Han, J.S., Kwak, E.Y., Lee, H.B., Shin, J.H., Baek, S.H., Chung, B.H., Kim, E.K., 2004. Manufacturing protein-DNA chip for depigmenting agent. J. Soc. Cosmet. Scientists Korea, 479–483. Parvez, S., Kang, M., Chung, H.S., Cho, C., Hong, M.C., Shin, M.K., Bae, H., 2006. Survey and mechanism of skin depigmenting and lightening agents. Phytother. Res. 20, 921–934. Solano, F., Briganti, S., Picardo, M., Ghanem, G., 2006. Hypopigmenting agents: an updated review on biological, chemical and clinical aspects. Pigment Cell Res. 19 (December (6)), 550–571. S.H. Yang, 2007. Development of Protein Chip for HTS System of Depigmenting Agent Screening. Master Thesis. University of Inha, Incheon, Korea.
School of Life Sciences and Biotechnology, Korea University, Anam 5th Street, Seongbuk-Gu, Seoul 136-701, Republic of Korea
doi:10.1016/j.jbiotec.2008.07.205 I3-P-047 Decursin inhibits tumor necrosis factor-alpha-induced insulin resistance in C2C12 skeletal muscle cells Ji-Eun Kang ∗ , Ik-Hwan Kim School of Life Sciences and Biotechnology, Korea University, Anam 5th Street, Seongbuk-Gu, Seoul 136-70, Republic of Korea E-mail address: [email protected] (J.-E. Kang). Insulin resistance is a key feature of type 2 diabetes and is a characteristic of wide range of other clinical tests and experiments (Karim and Juleen, 2007). Systemic inflammation is also a feature of obesity and type 2 diabetes, raising the hypothesis that elevated cytokine levels may contribute to peripheral insulin resistance (Nicholas et al., 2006). In this study, the insulin resistance was established with the treatment of an inflammatory cytokine, tumor necrosis factor-alpha (TNF-alpha) against C2C12 skeletal muscle cells. The TNF-alpha treatment was well-validated experimental models for insulin resistance by impairing insulin signaling events involved in GLUT4 translocation and inducing ROS accumulation. We induced C2C12 differentiation by substituting the medium DMEM supplemented with 2% horse serum for 10% FBS. The development of TNF-alpha-induced insulin resistance will be determined by glucose uptake. Glucose transport is evaluated by measuring [3 H] 2-deoxy-d-glucose and cellular ROS levels are measured by using DCF-DA. Decursin is a compound containing coumarin ring isolated from a root of Angelica gigas (Kim et al., 2005). The effect of decursin sensitizing insulin signaling against the TNF-alpha-induced insulin resistance will be presented. The mechanism of action of decursin against insulin resistance also will be suggested.
Protective effect of decursin against PMA-induced cytotoxicity in lung cell fibroblast
E-mail address: [email protected] (A.-H. Kim). Protein kinase C (PKC) has become an important target for therapeutic intervention in cancer, cardiovascular, neurological, metabolic and immunological disorders and has been considered as a target of anticancer drug screening because it is the cellular receptor of tumor-promoting phorbol esters. Phorbol 12-myristate 13-acetate (PMA), the most commonly used phorbol ester, binds to PKC and activates it (Gelsher and Dale, 1989). The activated PKC causes an extremely wide range of effects in cells and tissues. In many cell types, including fibroblasts and T lymphocytes, phorbol esters stimulate cellular proliferation, suggesting a role for PKC activation in this process (Berry and Nishizuka, 1990; Nomura et al., 2007). Decursin, a PKC activator anticancer agent isolated from Angelica gigas was cytotoxic to cells (Kim et al., 2005; Yim et al., 2005). In this study, the effect of decursin in lung cell fibroblast and the mechanism of action against PMA will be protected. In order to examine the effect of decursin against PMA-induced cytotoxicity in lung cell fibroblast, cell viability, apoptosis, cell cycle and ROS levels were measured by FACS. To analyze signal pathways, western blot and immunocytochemistry would be presented. References Berry, N., Nishizuka, Y., 1990. Protein kinase C and T cell activation. Eur. J. Biochem. 189, 205–214. Gelsher, A., Dale, I.L., 1989. Protein kinase C—a novel target for rational anti-cancer drug design? Anticancer Drug Des. 4, 93–105. Kim, H.H., Bang, S.S., Choi, S.J., Han, H., Kim, I.H., 2005. Involvement of PKC and ROS in the cytotoxic mechanism of anti-leukemic decursin and its derivatives and their structure-activity relationship in human K562 erythroleukemia and U937 myeloleukemia cells. Cancer Lett. 223, 191–201. Nomura, N., Nomura, M., Takahira, M., Sugiyama, K., 2007. Phorbol 12-myristate 13-acetate-activated protein kinase C increased migratory activity of subconjunctival fibroblasts via stress-activated protein kinase pathways. Mol. Vis. 13, 2320–2327. Yim, D.S., Singh, R.P., Agarwal, C., Lee, S.Y., Choi, H.J., Agarwal, R., 2005. A novel anticancer agent, decursin, induces G1 arrest and apoptosis in human prostate carcinoma cells. Cancer Res. 65, 1035–1044.
doi:10.1016/j.jbiotec.2008.07.207 I3-P-051 Styraxjaponoside C mediates its antifungal activity via apoptosis
References
Cana Park ∗ , Woo Sang Sung, Juneyoung Lee, Dong Gun Lee ∗
Karim, B., Juleen, R.Z., 2007. MAP4K4 Gene silencing in human skeletal muscle prevents tumor necrosis factor-induced insulin resistance. J. Biol. Chem. 282, 7783–7789. Kim, H.H., Sik, B.S., Seok, C.J., Han, H., Kim, I.H., 2005. Involvement of PKC and ROS in the cytotoxic mechanism of anti-leukemic decursin and its derivatives and their structure activity relation ship in human K562 erythroleukemia and U937 myeloleukemia cells. Cancer Lett. 223, 191–201. Nicholas, H., Evan, D.R., Eric, S.L., 2006. Reactive oxygen species have a causal role in multiple forms of insulin resistance. Nature 440, 944–994.
School of Life Sciences & Biotechnology, College of Natural Sciences, Kyungpook National University, Daegu, Republic of Korea
doi:10.1016/j.jbiotec.2008.07.206
E-mail address: [email protected] (C. Park). Styraxjaponoside C is a novel glycoside isolated from the stem bark of Styrax japonica S. et Z., which is one of the lignans (Kim et al., 2007). In this study, we investigated novel antimicrobial effects and its mode of action of Styraxjaponoside C. The result showed that Styraxjaponoside C exhibited antimicrobial activity against human pathogenic microorganisms without hemolytic effect on human erythrocytes. To elucidate the antimicrobial effects of Styraxjaponoside C, its effects on the dimorphism of Candida albicans induced by fetal bovine serum (FBS), which plays a key role in the pathogenesis of a host invasion, were