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Dedifferentiated liposarcoma with homologous lipoblastic differentiation
IAP 2014 ABSTRACTS
Rhabdomyosarcoma (RMS) is the most common soft tissue sarcoma among children. The identification of cancer stem cells (CSCs) represents one of the very relevant research topics because CSCs play important roles in tumor initiation and progression, as well as during metastasis formation and relapse of the disease. We examined the expression of several putative CSCs markers in tumor tissue sample obtained from 16-years-old female patient suffering from embryonal RMS and in the derived NSTS-11 cell line. This cell line was injected into immunodeficient mice and cell lines derived from all arising tumors were repeatedly xenografted. Both xenograft-induced tumors and respective cell lines were also analysed. Immunohistochemistry and immunofluorescence were used to examine FFPE samples; cell lines were analyzed by immunofluorescence, flow cytometry, and RT-PCR. We detected the following CSCs markers: CXCR4, CD133, nestin, ALDH, OCT3/4, nucleostemin, SOX2. Expression of these markers showed an increase in xenografted cell lines when compared with NSTS-11 cells; these results indicate an extended proportion of cells with CSCs phenotype during the process of repeated tumorigenicity testing. This study was supported by grants IGA MZCR NT13443-4, MUNI/C/0956/2012 and by the European Regional Development Fund and the State Budget of the Czech Republic (RECAMO, CZ.1.05/2.1.00/03.0101).
S49
as a large retrorectal or sacrococcygeal mass. We report a case of a 45-year-old woman with radicular symptoms, unsteady gait and urinary incontinence associated with a sacrococcygeal mass chordoma. Histologic sections showed aggregates of cells separated into lobules by an extensive amount of mucoid intercellular tissue and thin fibrous septa. Some of the tumor cells appearing physaliferous, were extremely large, with vacuolated cytoplasm, hyperchromatic and vesicular nuclei with prominent nucleoli. Other tumor cells were small, with inconspicuous nuclei and mitoses are sparse. The tumor showed positive reactivity to S100, EMA and AE1/AE3 antibodies, and negativity to GFAP, CK-7, CK-20 and Desmin. To the best of our knowledge, chordoma has not shown to have occurred in Southeast Nigeria with an over 20 million population as evidenced by dearth of documentations in the literature.
Bone and Soft Tissue Pathology: Poster#020 DEDIFFERENTIATED LIPOSARCOMA WITH HOMOLOGOUS LIPOBLASTIC DIFFERENTIATION Joon Hyuk Choi1, Jae Hwang Kim2 and Jae Chun Chang3 1Department of Pathology, 2Department of Surgery, and 3Department of Radiology, Yeungnam University College of Medicine, Daegu, South Korea
Bone and Soft Tissue Pathology: Poster#018 CHONDROID TENOSYNOVIAL GIANT CELL TUMOUR OF THE TEMPORO-MANDIBULAR JOINT: A CASE REPORT WITH REVIEW OF THE LITERTURE Ngendahayo Placide and Myant Nathalie Institut de Pathologie et Ge´ne´tique, Gosselies, Belgium Localised tenosynovial giant-cell tumour (TGCT) is one of the most common soft tissue tumours of the hand (finger joints and tendon sheaths) but is exceedingly rare in the head or neck. Chondroid metaplasia in TGCT is also rare and very few cases have been described. The aothors report a case of a well circumscribed nodule occurring in the temporo-mandibular joint of a 74-year-old woman and with histological and immunohistochemical features of chondroid tenosynovial giant-cell tumour. The presence of dystrophic lace-like calcifications can mimick the so-called chicken-wire calcifications found in chondrolastoma. The differential diagnosis is dicussed and a review of the literature is presented.
Bone and Soft Tissue Pathology: Poster#019 CHORDOMA: A RARITY IN SOUTH-EAST NIGERIA Chukwudi Onyeaghana Okani1 and Chimezie Madubogwu2 1Department of Histopathology, Benue State University Teaching Hospital Makurdi, Benue state, Nigeria, and 2Department of Surgery, Anambra State University Teaching Hospital Amaku Awka, Anambra State, Nigeria Chordomas originate from embryonic remnant of the notochord. They are primary bone neoplasms located on the midline from the skull base to the sacrum. Chordomas are slow growing, locally aggressive tumor with predilection for the spheno-occipital region of the skull base and sacral regions. The usual clinical manifestations of chordoma are insidious and nonspecific, making late presentation of patients inevitable. Chordomas arising in the sacrum may appear
Dedifferentiated liposarcoma is a distinct subtype of liposarcoma showing by transition from an atypical lipomatous tumor/well differentiated liposarcoma to non-lipogenic sarcoma of variable histologic grade. Recent studies have shown that the dedifferentiated component can show lipoblastic differentiation. We describe a case of dedifferentiated liposarcoma with homologous lipoblastic differentiation arising from the abdominal cavity. The patient was a 67-year-old woman who presented with the left lower quadrant abdominal mass for one month. Abdominal CT scan showed a well defined mass with heterogeneous signal intensity in the abdominal cavity. Excision of the mass was performed. Grossly, the mass measured 20 12 6 cm. The cut surface was well circumscribed, yellow-brown, rubbery soft, with focal hemorrhage. Histologically, the tumor showed spindle and pleomorphic tumor cells in myxoid stroma with scattered lipoblasts. In addition, there were smaller foci of well differentiated liposarcoma showing atypical hyperchromatic stromal cells. The tumor cells in the dedifferentiated component were immunoreactive for MDM2 and CDK4. Fluorescence in situ hybridization showed high-level amplification of MDM2. Dedifferentiated liposarcomas with homologous lipoblastic differentiation should not be confused with pleomorphic liposarcomas and other types of pleomorphic sarcomas with fat component. We describe the histopathological features of liposarcomas. Bone and Soft Tissue Pathology: Poster#021 ISOLATED CALCIFIED AMYLOIDOMA OF THE ABDOMINAL WALL MIMICKING SARCOMA Pouneh Dokouhaki1, Peter Graham2 and Rani Kanthan1 1Department of Pathology & Lab Medicine, and 2Department of Surgery, University of Saskatchewan, Canada Amyloidoma is a localized tumoral deposition of amyloid protein without systemic involvement. Though rare, it has been reported in various tissues. Calcified amyloidoma is an extremely uncommon condition that can mimic benign and malignant soft tissue tumors.
Copyright © Royal College of pathologists of Australasia. Unauthorized reproduction of this article is prohibited.
Rhabdomyosarcoma (RMS) is the most common soft tissue sarcoma among children. The identification of cancer stem cells (CSCs) represents one of the very relevant research topics because CSCs play important roles in tumor initiation and progression, as well as during metastasis formation and relapse of the disease. We examined the expression of several putative CSCs markers in tumor tissue sample obtained from 16-years-old female patient suffering from embryonal RMS and in the derived NSTS-11 cell line. This cell line was injected into immunodeficient mice and cell lines derived from all arising tumors were repeatedly xenografted. Both xenograft-induced tumors and respective cell lines were also analysed. Immunohistochemistry and immunofluorescence were used to examine FFPE samples; cell lines were analyzed by immunofluorescence, flow cytometry, and RT-PCR. We detected the following CSCs markers: CXCR4, CD133, nestin, ALDH, OCT3/4, nucleostemin, SOX2. Expression of these markers showed an increase in xenografted cell lines when compared with NSTS-11 cells; these results indicate an extended proportion of cells with CSCs phenotype during the process of repeated tumorigenicity testing. This study was supported by grants IGA MZCR NT13443-4, MUNI/C/0956/2012 and by the European Regional Development Fund and the State Budget of the Czech Republic (RECAMO, CZ.1.05/2.1.00/03.0101).
S49
as a large retrorectal or sacrococcygeal mass. We report a case of a 45-year-old woman with radicular symptoms, unsteady gait and urinary incontinence associated with a sacrococcygeal mass chordoma. Histologic sections showed aggregates of cells separated into lobules by an extensive amount of mucoid intercellular tissue and thin fibrous septa. Some of the tumor cells appearing physaliferous, were extremely large, with vacuolated cytoplasm, hyperchromatic and vesicular nuclei with prominent nucleoli. Other tumor cells were small, with inconspicuous nuclei and mitoses are sparse. The tumor showed positive reactivity to S100, EMA and AE1/AE3 antibodies, and negativity to GFAP, CK-7, CK-20 and Desmin. To the best of our knowledge, chordoma has not shown to have occurred in Southeast Nigeria with an over 20 million population as evidenced by dearth of documentations in the literature.
Bone and Soft Tissue Pathology: Poster#020 DEDIFFERENTIATED LIPOSARCOMA WITH HOMOLOGOUS LIPOBLASTIC DIFFERENTIATION Joon Hyuk Choi1, Jae Hwang Kim2 and Jae Chun Chang3 1Department of Pathology, 2Department of Surgery, and 3Department of Radiology, Yeungnam University College of Medicine, Daegu, South Korea
Bone and Soft Tissue Pathology: Poster#018 CHONDROID TENOSYNOVIAL GIANT CELL TUMOUR OF THE TEMPORO-MANDIBULAR JOINT: A CASE REPORT WITH REVIEW OF THE LITERTURE Ngendahayo Placide and Myant Nathalie Institut de Pathologie et Ge´ne´tique, Gosselies, Belgium Localised tenosynovial giant-cell tumour (TGCT) is one of the most common soft tissue tumours of the hand (finger joints and tendon sheaths) but is exceedingly rare in the head or neck. Chondroid metaplasia in TGCT is also rare and very few cases have been described. The aothors report a case of a well circumscribed nodule occurring in the temporo-mandibular joint of a 74-year-old woman and with histological and immunohistochemical features of chondroid tenosynovial giant-cell tumour. The presence of dystrophic lace-like calcifications can mimick the so-called chicken-wire calcifications found in chondrolastoma. The differential diagnosis is dicussed and a review of the literature is presented.
Bone and Soft Tissue Pathology: Poster#019 CHORDOMA: A RARITY IN SOUTH-EAST NIGERIA Chukwudi Onyeaghana Okani1 and Chimezie Madubogwu2 1Department of Histopathology, Benue State University Teaching Hospital Makurdi, Benue state, Nigeria, and 2Department of Surgery, Anambra State University Teaching Hospital Amaku Awka, Anambra State, Nigeria Chordomas originate from embryonic remnant of the notochord. They are primary bone neoplasms located on the midline from the skull base to the sacrum. Chordomas are slow growing, locally aggressive tumor with predilection for the spheno-occipital region of the skull base and sacral regions. The usual clinical manifestations of chordoma are insidious and nonspecific, making late presentation of patients inevitable. Chordomas arising in the sacrum may appear
Dedifferentiated liposarcoma is a distinct subtype of liposarcoma showing by transition from an atypical lipomatous tumor/well differentiated liposarcoma to non-lipogenic sarcoma of variable histologic grade. Recent studies have shown that the dedifferentiated component can show lipoblastic differentiation. We describe a case of dedifferentiated liposarcoma with homologous lipoblastic differentiation arising from the abdominal cavity. The patient was a 67-year-old woman who presented with the left lower quadrant abdominal mass for one month. Abdominal CT scan showed a well defined mass with heterogeneous signal intensity in the abdominal cavity. Excision of the mass was performed. Grossly, the mass measured 20 12 6 cm. The cut surface was well circumscribed, yellow-brown, rubbery soft, with focal hemorrhage. Histologically, the tumor showed spindle and pleomorphic tumor cells in myxoid stroma with scattered lipoblasts. In addition, there were smaller foci of well differentiated liposarcoma showing atypical hyperchromatic stromal cells. The tumor cells in the dedifferentiated component were immunoreactive for MDM2 and CDK4. Fluorescence in situ hybridization showed high-level amplification of MDM2. Dedifferentiated liposarcomas with homologous lipoblastic differentiation should not be confused with pleomorphic liposarcomas and other types of pleomorphic sarcomas with fat component. We describe the histopathological features of liposarcomas. Bone and Soft Tissue Pathology: Poster#021 ISOLATED CALCIFIED AMYLOIDOMA OF THE ABDOMINAL WALL MIMICKING SARCOMA Pouneh Dokouhaki1, Peter Graham2 and Rani Kanthan1 1Department of Pathology & Lab Medicine, and 2Department of Surgery, University of Saskatchewan, Canada Amyloidoma is a localized tumoral deposition of amyloid protein without systemic involvement. Though rare, it has been reported in various tissues. Calcified amyloidoma is an extremely uncommon condition that can mimic benign and malignant soft tissue tumors.
Copyright © Royal College of pathologists of Australasia. Unauthorized reproduction of this article is prohibited.