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Dendritic Cell Subtypes in Potentially Malignant Disorders and Squamous Cell Carcinoma of the Oral Cavity and Lip
OOOO Volume 120, Number 2 PPC-001 - CHARACTERIZATION OF TUMOR-ASSOCIATED MACROPHAGES SUBPOPULATION M1 AND THEIR RELATIONSHIP WITH CLINICOPATHOLOGICAL PARAMETERS IN LOWER LIP SQUAMOUS CELL CARCINOMAS. ARLLEY DE SOUSA LEITÃO, PRISCILLA SUASSUNA CARNEIRO LÚCIO, DANIELA COTTA RIBEIRO, MARIA CÁSSIA FERREIRA DE AGUIAR, POLLIANNA MUNIZ ALVES, CASSIANO FRANCISCO WEEGE NONAKA, GUSTAVO PINA GODOY. UNIVERSIDADE ESTADUAL DA PARAÍBA. Objective: To analyze the presence of tumor-associated macrophages (TAMs), subpopulation M1, in lower lip squamous cell carcinoma (LLSCC) by immunohistochemistry and to evaluate the possible association of this cell subtype with clinicopathological features of the tumor. Materials and Methods: Forty-two cases of LLSCC were evaluated. The histopathological grade of malignancy was determined, and the material was submitted to double staining with anti-CD68/anti-HLA-DR antibodies. Results: Analysis along the invasive tumor front revealed the presence of M1 TAMs. The numbers of M1 macrophages were greater in T2eT4 (large) tumors than in T1 tumors (p ¼ 0.031), as well as being greater in high-grade tumors than in lowgrade tumors (p ¼ 0.023). Conclusions: The significant differences observed for the M1 macrophage phenotype suggest the participation of these cells in the inhibition of LLSCC progression, because M1 TAMs are able to destroy neoplastic cells.
PPC-002 - DENDRITIC CELL SUBTYPES IN POTENTIALLY MALIGNANT DISORDERS AND SQUAMOUS CELL CARCINOMA OF THE ORAL CAVITY AND LIP. JORGE ESQUICHE LEÓN, LUCIANA YAMAMOTO DE ALMEIDA, SUZIE APARECIDA LACERDA, LUIZ GUILHERME BRENTEGANI, CHRISTIANO DE OLIVEIRA SANTOS. FACULDADE DE ODONTOLOGIA DE RIBEIRÃO PRETO e USP. Objective: Dendritic cell (DC) subtypes play a critical role in generating anti-tumor immunity. Little is known about DC subtype infiltration in potentially malignant disorders (PMDs) and squamous cell carcinomas (SCCs). Materials and Methods: Immature DC (imDC: S-100, CD1a, and CD207), mature DC (mDC: CD83 and CD208), CD11c, and CD123 immunomarkers were assessed in normal lip mucosa (n ¼ 15), oral leukoplakia (OL; n ¼ 24), actinic cheilitis (AC; n ¼ 16), oral cavity SCC (OSCC; n ¼ 17) and lower lip SCC (LLSCC; n ¼ 10). Results: The number of DCs was higher in OL than in AC, as well as being higher in LLSCC than in OSCC; PMDs presented more imDCs than mDCs, which were proportionally more common in SCCs (p < 0.05). A similar increase was observed for CD11c+ cells, the most common among DC subtypes. CD123+ cells were scarce. Conclusions: Our results suggest a failure of DC function in oral/lip carcinogenesis. This could explain why dysplastic or cancer cells grow despite the presence of infiltrating immune cells.
PPC-003 - QUALITY OF LIFE ASSESSMENT IN PATIENTS WITH HEAD AND NECK CANCER. ROGÉRIA LUCIO DE OLIVEIRA, RAFAELLE FERREIRA DOS SANTOS, SÉRGIO HENRIQUE GONÇALVES DE CARVALHO, SANDRA
ABSTRACTS Abstracts e83 APARECIDA MARINHO, TÁSSIA CRISTINA DE ALMEIDA PINTO SARMENTO, DMITRY JOSÉ DE SANTANA SARMENTO. UNIVERSIDADE ESTADUAL DA PARAÍBA. Objective: To evaluate the quality of life of patients with head and neck cancer at a referral hospital in northeastern Brazil. Materials and Methods: A qualitative, transversal, prospective study was performed. The survey instrument was the University of Washington Quality of Life Questionnaire (UWQoL). Data were analyzed with descriptive analysis. Results: Most patients (64%) were male, with mean age of 66.76 11.45 years. The most prevalent type of cancer was squamous cell carcinoma (72%). The domains in which there was the greatest impairment were pain (68% had some pain), appearance (60% reported some change), and anxiety (52% showed some change). With respect to the matters that the patients believed more important during the last 7 days, those most cited were pain (by 52%) and appearance (by 36%). Conclusions: There was a higher prevalence of males and of squamous cell carcinoma. In this quality of life assessment, pain, appearance, and anxiety were the domains that were most affected.
PPC-004 - BUILDING AN ORAL CANCER PATHWAY: THE RIGHT TO INFORMATION FOR COMPREHENSIVE CARE. MARIANNE DE VASCONCELOS CARVALHO, ALLAN VINÍCIUS MARTINS DE BARROS, ANA MARIA IPÓLITO BARROS, STEFÂNIA JERONIMO FERREIRA, TEREZA CRISTINA CORREIA, LUIZ GUTENBERG TOLEDO DE MIRANDA COELHO JUNIOR, LEONARDO CARNUT. UNIVERSIDADE DE PERNAMBUCO e CAMPUS ARCOVERDE. Objective: To develop a referral pathway for suspected lesions associated with an oral cancer screening program. Materials and Methods: A referral pathway was designed through the technique of face validity (consensus among 4 experts) and organization of flows through theoretical and logical models. An oral cancer screening program was also executed, because it is an effective strategy, together with a referral pathway, to promote early detection and reduce costs, as well as to reduce rates of morbidity and mortality. Results: The final oral cancer referral pathway comprised 5 centers at 3 different levels of health care, with 36 different pathways. Conclusions: The referral pathway is a statement of consensus based on current evidence and approaches to the management of oral cancer and suspected lesions. It promotes a consistent and standardized approach to managing care, to ensure that people affected by oral cancer experience coordinated care.
PPC-005 - CRACK USERS: SOCIODEMOGRAPHIC PROFILE, VIRAL INFECTIONS, AND ORAL HEALTH. DÉBORAH DANIELLA DINIZ FONSÊCA, IGOR HENRIQUE MORAIS SILVA, ANDREZA BARKOKEBAS SANTOS DE FARIA, LUIZ ALCINO MONTEIRO GUEIROS, ALESSANDRA ALBUQUERQUE TAVARES CARVALHO, JAIR CARNEIRO LEÃO. UNIVERSIDADE FEDERAL DE PERNAMBUCO. Objective: To evaluate the sociodemographic and oral health profiles, as well as seroprevalence of HIV, HCV and HBV in crack cocaine users. Materials and Methods: Ninety-four individuals were examined. The oral health evaluation included the DMFT and CPI indices. Unstimulated salivary flow rate, xerostomia, and oral lesions were also evaluated.
PPC-002 - DENDRITIC CELL SUBTYPES IN POTENTIALLY MALIGNANT DISORDERS AND SQUAMOUS CELL CARCINOMA OF THE ORAL CAVITY AND LIP. JORGE ESQUICHE LEÓN, LUCIANA YAMAMOTO DE ALMEIDA, SUZIE APARECIDA LACERDA, LUIZ GUILHERME BRENTEGANI, CHRISTIANO DE OLIVEIRA SANTOS. FACULDADE DE ODONTOLOGIA DE RIBEIRÃO PRETO e USP. Objective: Dendritic cell (DC) subtypes play a critical role in generating anti-tumor immunity. Little is known about DC subtype infiltration in potentially malignant disorders (PMDs) and squamous cell carcinomas (SCCs). Materials and Methods: Immature DC (imDC: S-100, CD1a, and CD207), mature DC (mDC: CD83 and CD208), CD11c, and CD123 immunomarkers were assessed in normal lip mucosa (n ¼ 15), oral leukoplakia (OL; n ¼ 24), actinic cheilitis (AC; n ¼ 16), oral cavity SCC (OSCC; n ¼ 17) and lower lip SCC (LLSCC; n ¼ 10). Results: The number of DCs was higher in OL than in AC, as well as being higher in LLSCC than in OSCC; PMDs presented more imDCs than mDCs, which were proportionally more common in SCCs (p < 0.05). A similar increase was observed for CD11c+ cells, the most common among DC subtypes. CD123+ cells were scarce. Conclusions: Our results suggest a failure of DC function in oral/lip carcinogenesis. This could explain why dysplastic or cancer cells grow despite the presence of infiltrating immune cells.
PPC-003 - QUALITY OF LIFE ASSESSMENT IN PATIENTS WITH HEAD AND NECK CANCER. ROGÉRIA LUCIO DE OLIVEIRA, RAFAELLE FERREIRA DOS SANTOS, SÉRGIO HENRIQUE GONÇALVES DE CARVALHO, SANDRA
ABSTRACTS Abstracts e83 APARECIDA MARINHO, TÁSSIA CRISTINA DE ALMEIDA PINTO SARMENTO, DMITRY JOSÉ DE SANTANA SARMENTO. UNIVERSIDADE ESTADUAL DA PARAÍBA. Objective: To evaluate the quality of life of patients with head and neck cancer at a referral hospital in northeastern Brazil. Materials and Methods: A qualitative, transversal, prospective study was performed. The survey instrument was the University of Washington Quality of Life Questionnaire (UWQoL). Data were analyzed with descriptive analysis. Results: Most patients (64%) were male, with mean age of 66.76 11.45 years. The most prevalent type of cancer was squamous cell carcinoma (72%). The domains in which there was the greatest impairment were pain (68% had some pain), appearance (60% reported some change), and anxiety (52% showed some change). With respect to the matters that the patients believed more important during the last 7 days, those most cited were pain (by 52%) and appearance (by 36%). Conclusions: There was a higher prevalence of males and of squamous cell carcinoma. In this quality of life assessment, pain, appearance, and anxiety were the domains that were most affected.
PPC-004 - BUILDING AN ORAL CANCER PATHWAY: THE RIGHT TO INFORMATION FOR COMPREHENSIVE CARE. MARIANNE DE VASCONCELOS CARVALHO, ALLAN VINÍCIUS MARTINS DE BARROS, ANA MARIA IPÓLITO BARROS, STEFÂNIA JERONIMO FERREIRA, TEREZA CRISTINA CORREIA, LUIZ GUTENBERG TOLEDO DE MIRANDA COELHO JUNIOR, LEONARDO CARNUT. UNIVERSIDADE DE PERNAMBUCO e CAMPUS ARCOVERDE. Objective: To develop a referral pathway for suspected lesions associated with an oral cancer screening program. Materials and Methods: A referral pathway was designed through the technique of face validity (consensus among 4 experts) and organization of flows through theoretical and logical models. An oral cancer screening program was also executed, because it is an effective strategy, together with a referral pathway, to promote early detection and reduce costs, as well as to reduce rates of morbidity and mortality. Results: The final oral cancer referral pathway comprised 5 centers at 3 different levels of health care, with 36 different pathways. Conclusions: The referral pathway is a statement of consensus based on current evidence and approaches to the management of oral cancer and suspected lesions. It promotes a consistent and standardized approach to managing care, to ensure that people affected by oral cancer experience coordinated care.
PPC-005 - CRACK USERS: SOCIODEMOGRAPHIC PROFILE, VIRAL INFECTIONS, AND ORAL HEALTH. DÉBORAH DANIELLA DINIZ FONSÊCA, IGOR HENRIQUE MORAIS SILVA, ANDREZA BARKOKEBAS SANTOS DE FARIA, LUIZ ALCINO MONTEIRO GUEIROS, ALESSANDRA ALBUQUERQUE TAVARES CARVALHO, JAIR CARNEIRO LEÃO. UNIVERSIDADE FEDERAL DE PERNAMBUCO. Objective: To evaluate the sociodemographic and oral health profiles, as well as seroprevalence of HIV, HCV and HBV in crack cocaine users. Materials and Methods: Ninety-four individuals were examined. The oral health evaluation included the DMFT and CPI indices. Unstimulated salivary flow rate, xerostomia, and oral lesions were also evaluated.