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Detection of anti-Borna disease virus antibodies by Western blot analysis. Reply
Psychiatry Research 134 (2005) 106 www.elsevier.com/locate/psychres
Correspondence Detection of anti-Borna disease virus antibodies by Western blot analysis. Reply We thank Dr. Ghosh and associates for their useful comments on our article and the responses are as follows. (1) Readers may find two other references useful for background information on Borna disease (Nakamura et al., 2000; Rott and Becht, 1995). (2–3) The BDV antigens used for Western blotting were the horse BDV-derived recombinant full-length p10 (X protein) fusion proteins with GST (glutathione S-transferase), and the negative control antigen was GST alone (Kitani et al., 1996; Ludwig et al., 1993; Malik et al., 1999; Nakaya et al., 1996; Wolff et al., 2000).] (p. 202 lines 51–57) (Terayama et al., 2003). (4) We hope that the following additional information is helpful in understanding our Fig. 1: Lanes 2 and 3 showed representative results obtained by use of anti-BDV-p10 antibodypositive serum. Lanes 4 and 5 showed representative results obtained by use of anti-BDV-p10 antibodynegative serum. It has shifted from GST-p10 35 000 Da to GST-p10. (5) We very much regret the omission of Dr. Ghosh’s name from the Malik et al. reference in our bibliography and our accidental confusion of the first and last names in the Wolff reference. The corrected references are given below.
References Malik, T.H., Kobayashi, T., Ghosh, M., Kishi, M., Lai, P.K., 1999. Nuclear localization of the protein from the open reading frame 1 of the Borna disease virus was through interactions with the viral nucleoprotein. Virology 258, 65 – 72.
Nakamura, Y., Takahashi, H., Shoya, Y., Nakaya, T., Watanabe, M., Tomonaga, K., Iwahashi, K., Ameno, K., Momiyama, N., Taniyama, H., Sata, T., Kurata, T., de la Torre, J.C., Ikuta, K., 2000. Isolation of Borna disease virus from human brain tissue. Journal of Virology 74, 4601 – 4611. Rott, R., Becht, H., 1995. Natural and experimental Borna disease in animals. In: Koprowki, H., Lipkin, W.I. (Eds.), Borna Disease Virus. Springer-Verlag KG, Berlin, pp. 17 – 30. Terayama, H., Nishino, Y., Kishi, M., Ikuta, K., Itoh, M., Iwahashi, K., 2003. Detection of anti-Borna Disease Virus (BDV) antibodies from patients with schizophrenia and mood disorders in Japan. Psychiatry Research 120, 201 – 206. Wolff, T., Pfleger, R., Wehner, T., Reinhardt, J., Richt, J.A., 2000. A short leucine-rich sequence in the Borna disease virus p10 protein mediates association with the viral phospho- and nucleoprotein. Journal of General Virology 81, 939 – 947.
Hayato Terayamaa Yoshii Nishinob Masahiko Kishic Mashiro Itoha Kazuyoshi Ikutad Kazuhiko Iwahashie,T a
First Department of Anatomy, School of Tokyo Medical University, Tokyo, Japan b Research Institute of Biosciences, Azabu University, Kanagawa, Japan c Central Laboratories, Kyoritsu Shoji Corporation, Ibaraki, Japan d Department of Virology Research, Institute for Microbial Diseases, Osaka University, Osaka, Japan e Department of Neurophysiology, Graduate School of Azabu University, 1-71-1 Futinobe, Sagamihara-shi, Kanagawa 229-8501, Japan TCorresponding author. Tel./fax: +81 42 769 1930. E-mail address: [email protected].
DOI of original article: 10.1016/j.psychres.2004.10.002. 0165-1781/$ - see front matter D 2005 Published by Elsevier Ireland Ltd. doi:10.1016/j.psychres.2005.03.001
Correspondence Detection of anti-Borna disease virus antibodies by Western blot analysis. Reply We thank Dr. Ghosh and associates for their useful comments on our article and the responses are as follows. (1) Readers may find two other references useful for background information on Borna disease (Nakamura et al., 2000; Rott and Becht, 1995). (2–3) The BDV antigens used for Western blotting were the horse BDV-derived recombinant full-length p10 (X protein) fusion proteins with GST (glutathione S-transferase), and the negative control antigen was GST alone (Kitani et al., 1996; Ludwig et al., 1993; Malik et al., 1999; Nakaya et al., 1996; Wolff et al., 2000).] (p. 202 lines 51–57) (Terayama et al., 2003). (4) We hope that the following additional information is helpful in understanding our Fig. 1: Lanes 2 and 3 showed representative results obtained by use of anti-BDV-p10 antibodypositive serum. Lanes 4 and 5 showed representative results obtained by use of anti-BDV-p10 antibodynegative serum. It has shifted from GST-p10 35 000 Da to GST-p10. (5) We very much regret the omission of Dr. Ghosh’s name from the Malik et al. reference in our bibliography and our accidental confusion of the first and last names in the Wolff reference. The corrected references are given below.
References Malik, T.H., Kobayashi, T., Ghosh, M., Kishi, M., Lai, P.K., 1999. Nuclear localization of the protein from the open reading frame 1 of the Borna disease virus was through interactions with the viral nucleoprotein. Virology 258, 65 – 72.
Nakamura, Y., Takahashi, H., Shoya, Y., Nakaya, T., Watanabe, M., Tomonaga, K., Iwahashi, K., Ameno, K., Momiyama, N., Taniyama, H., Sata, T., Kurata, T., de la Torre, J.C., Ikuta, K., 2000. Isolation of Borna disease virus from human brain tissue. Journal of Virology 74, 4601 – 4611. Rott, R., Becht, H., 1995. Natural and experimental Borna disease in animals. In: Koprowki, H., Lipkin, W.I. (Eds.), Borna Disease Virus. Springer-Verlag KG, Berlin, pp. 17 – 30. Terayama, H., Nishino, Y., Kishi, M., Ikuta, K., Itoh, M., Iwahashi, K., 2003. Detection of anti-Borna Disease Virus (BDV) antibodies from patients with schizophrenia and mood disorders in Japan. Psychiatry Research 120, 201 – 206. Wolff, T., Pfleger, R., Wehner, T., Reinhardt, J., Richt, J.A., 2000. A short leucine-rich sequence in the Borna disease virus p10 protein mediates association with the viral phospho- and nucleoprotein. Journal of General Virology 81, 939 – 947.
Hayato Terayamaa Yoshii Nishinob Masahiko Kishic Mashiro Itoha Kazuyoshi Ikutad Kazuhiko Iwahashie,T a
First Department of Anatomy, School of Tokyo Medical University, Tokyo, Japan b Research Institute of Biosciences, Azabu University, Kanagawa, Japan c Central Laboratories, Kyoritsu Shoji Corporation, Ibaraki, Japan d Department of Virology Research, Institute for Microbial Diseases, Osaka University, Osaka, Japan e Department of Neurophysiology, Graduate School of Azabu University, 1-71-1 Futinobe, Sagamihara-shi, Kanagawa 229-8501, Japan TCorresponding author. Tel./fax: +81 42 769 1930. E-mail address: [email protected].
DOI of original article: 10.1016/j.psychres.2004.10.002. 0165-1781/$ - see front matter D 2005 Published by Elsevier Ireland Ltd. doi:10.1016/j.psychres.2005.03.001