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Detection of human papillomavirus in squamous cell carcinoma
P220
OtolaryngologyHead and Neck Surgery August T999
Scientific Posters
geal lipomas. Additionally, this patient's unique ENT syndrome will be discussed with a literature review describing the entity. Results: Benign symmetric lipomatosis, Madelung's disease, and Lannois-Bensaude syndrome are all names used to describe the same rare subgroup of lipomatous disease. The disease involves a marked symmetric and disproportionate accumulation of normal subcutaneous adipose tissue. The distribution is characteristic, occurring behind the ears, on the submandibular and supraclavicular areas, as well as on the shoulders, resulting in a 'horse collar' appearance. Since its first description in 1888, some 200 cases have been reported. The etiology of this disease is unknown. Treatment is limited to surgical removal for cosmetic reasons or to resolve the pain sometimes associated with it. The larynx and hypopharynx are relatively immune to the presence of lipomas, and the intrinsic form of lipoma in these regions is rare. Until 1994 less then 90 cases of laryngeal and hypopharyngeal lipomas had been reported in the world literature. The larynx and hypopharynx are not more prone to lipomas in cases of multiple lipomatosis. There have been rare reports in the literature of intrinsic laryngeal/hypopharyngeal lipomas occurring in patients with diffuse lipomatosis. Only 2 of these cases had a diagnosis of and clinical characteristics consistent with Launois-Bensaude syndrome. This case represents the third.
58
Detection of Human Papillomavirus in Squamous Cell Carcinoma VIVIAN MAC) MD; OREN FRIEDMAN MD; WILLIAM M KEANE MD; Philadelphia PA
Objectives: To present 2 interesting cases of squamous cell carcinoma and to evaluate for the presence of human papillomavirus (HPV) as a possible viral etiology. Methods: Two interesting cases of squamous cell carcinoma (SCCA) are presented. The first is the only known case of an SCCA arising in a tongue base papilloma, and the second is an aggressive SCCA of the larynx that presented in a 17year-old girl. Tumor specimens were analyzed by polymerase chain reaction (PCR) for the presence of HPV DNA. Results: PCR analysis revealed the presence of HPV-16 DNA in both tumor specimens. HPV types 6, 11, and 18 were not detected. Conclusion: HPV type 16 has been implicated in SCCA of the head and neck. This report details 2 more cases of SCCA in which HPV 16 is implicated as a causative agent and lends support to the possible role of HPV in tumorigenesis. 59
Serum Concentrations of Cytokines in Kumura's Disease NOBUO OHTA MD; SHIGERV FUKASEMD; MASARU AOYAGI MD; Yamagata Japan
Objectives: Kimura's disease is a chronic disease characterized histologically by granulomas with massive eosinophilic infiltration. IL-4, IL-5, and GM-CSF are known as eosinophilopoletins. To examine the role of these cytokines in inductive eosinophilopoiesis, the concentrations of these cytokines were examined. Methods: A total of 14 sera from 8 patients with Kimura's disease were studied. The number of eosinophils in peripheral blood from patients with Kimura's disease were counted. The concentrations of IgE, ECP, IL-4, IL-5, IL-6, GM-CSF, and CD23 in serum from patients with Kimura's disease were measured by ELISA (EASIA Genzyme, Denmark, and Quantikine R&D Systems, USA, respectively). Results: Serum levels of IgE and ECP were significantly higher than those of control and those decreased after treatment. We could not detect IL-4, IL-5, IL-6, and GM-CSF in sera from patients with Kimura's disease. Conclusion: The question of what leads to eosinophilia in patients without IL-4, IL-5, and GM-CSF is still obscure. These results may allow us to speculate that eosinophilia in patients with Kimura's disease is not simply due to an increase of these cytokines in sera. 6O
Neoplasms of the Temporomandibular Joint: Diagnostic Dilemma SAM P MOSTAFAPOUR MD; NEAL D FUTRANMD DMD; Seaffle WA
Objectives: Neoplasms of the temporomandibular joint (TMJ) are rare. However, the presentation of these lesions usually mimics that of much more common causes of TMJ syndromes, such as pain, swelling, or click, leading to delay in diagnosis. In order to increase awareness of TMJ neoplasms and establish guidelines for early intervention, a retrospective analysis of our experience with a series of patients with neoplasms of the TMJ was performed. Methods: The study involved retrospective review of the records of patients with neoplasms of the TMJ from 1990 to 1997. Presenting symptoms, time to initial radiographic study, interventions, time to final pathologic diagnosis, and outcomes were examined. Results: Eight patients were identified. All were female and had unilateral primary neoplasms of the TMJ, which were initially diagnosed as "TMJ syndrome." The unusual neoplasms included benign (calcium pyrophosphate, dihydrate crystal deposition disease, or pseudogout) as well as malignant (chondrosarcoma) neoplasms. Time from initial presentation to final diagnosis was in most cases prolonged, ranging from 3 months to 8 years. Patients typically showed advanced lesions radiographically. All patients required surgical extirpation. Postoperative radiation therapy was used for malignant lesions. Patient outcomes are delineated. Conclusion: Neoplasms, both benign and malignant, of the
OtolaryngologyHead and Neck Surgery August T999
Scientific Posters
geal lipomas. Additionally, this patient's unique ENT syndrome will be discussed with a literature review describing the entity. Results: Benign symmetric lipomatosis, Madelung's disease, and Lannois-Bensaude syndrome are all names used to describe the same rare subgroup of lipomatous disease. The disease involves a marked symmetric and disproportionate accumulation of normal subcutaneous adipose tissue. The distribution is characteristic, occurring behind the ears, on the submandibular and supraclavicular areas, as well as on the shoulders, resulting in a 'horse collar' appearance. Since its first description in 1888, some 200 cases have been reported. The etiology of this disease is unknown. Treatment is limited to surgical removal for cosmetic reasons or to resolve the pain sometimes associated with it. The larynx and hypopharynx are relatively immune to the presence of lipomas, and the intrinsic form of lipoma in these regions is rare. Until 1994 less then 90 cases of laryngeal and hypopharyngeal lipomas had been reported in the world literature. The larynx and hypopharynx are not more prone to lipomas in cases of multiple lipomatosis. There have been rare reports in the literature of intrinsic laryngeal/hypopharyngeal lipomas occurring in patients with diffuse lipomatosis. Only 2 of these cases had a diagnosis of and clinical characteristics consistent with Launois-Bensaude syndrome. This case represents the third.
58
Detection of Human Papillomavirus in Squamous Cell Carcinoma VIVIAN MAC) MD; OREN FRIEDMAN MD; WILLIAM M KEANE MD; Philadelphia PA
Objectives: To present 2 interesting cases of squamous cell carcinoma and to evaluate for the presence of human papillomavirus (HPV) as a possible viral etiology. Methods: Two interesting cases of squamous cell carcinoma (SCCA) are presented. The first is the only known case of an SCCA arising in a tongue base papilloma, and the second is an aggressive SCCA of the larynx that presented in a 17year-old girl. Tumor specimens were analyzed by polymerase chain reaction (PCR) for the presence of HPV DNA. Results: PCR analysis revealed the presence of HPV-16 DNA in both tumor specimens. HPV types 6, 11, and 18 were not detected. Conclusion: HPV type 16 has been implicated in SCCA of the head and neck. This report details 2 more cases of SCCA in which HPV 16 is implicated as a causative agent and lends support to the possible role of HPV in tumorigenesis. 59
Serum Concentrations of Cytokines in Kumura's Disease NOBUO OHTA MD; SHIGERV FUKASEMD; MASARU AOYAGI MD; Yamagata Japan
Objectives: Kimura's disease is a chronic disease characterized histologically by granulomas with massive eosinophilic infiltration. IL-4, IL-5, and GM-CSF are known as eosinophilopoletins. To examine the role of these cytokines in inductive eosinophilopoiesis, the concentrations of these cytokines were examined. Methods: A total of 14 sera from 8 patients with Kimura's disease were studied. The number of eosinophils in peripheral blood from patients with Kimura's disease were counted. The concentrations of IgE, ECP, IL-4, IL-5, IL-6, GM-CSF, and CD23 in serum from patients with Kimura's disease were measured by ELISA (EASIA Genzyme, Denmark, and Quantikine R&D Systems, USA, respectively). Results: Serum levels of IgE and ECP were significantly higher than those of control and those decreased after treatment. We could not detect IL-4, IL-5, IL-6, and GM-CSF in sera from patients with Kimura's disease. Conclusion: The question of what leads to eosinophilia in patients without IL-4, IL-5, and GM-CSF is still obscure. These results may allow us to speculate that eosinophilia in patients with Kimura's disease is not simply due to an increase of these cytokines in sera. 6O
Neoplasms of the Temporomandibular Joint: Diagnostic Dilemma SAM P MOSTAFAPOUR MD; NEAL D FUTRANMD DMD; Seaffle WA
Objectives: Neoplasms of the temporomandibular joint (TMJ) are rare. However, the presentation of these lesions usually mimics that of much more common causes of TMJ syndromes, such as pain, swelling, or click, leading to delay in diagnosis. In order to increase awareness of TMJ neoplasms and establish guidelines for early intervention, a retrospective analysis of our experience with a series of patients with neoplasms of the TMJ was performed. Methods: The study involved retrospective review of the records of patients with neoplasms of the TMJ from 1990 to 1997. Presenting symptoms, time to initial radiographic study, interventions, time to final pathologic diagnosis, and outcomes were examined. Results: Eight patients were identified. All were female and had unilateral primary neoplasms of the TMJ, which were initially diagnosed as "TMJ syndrome." The unusual neoplasms included benign (calcium pyrophosphate, dihydrate crystal deposition disease, or pseudogout) as well as malignant (chondrosarcoma) neoplasms. Time from initial presentation to final diagnosis was in most cases prolonged, ranging from 3 months to 8 years. Patients typically showed advanced lesions radiographically. All patients required surgical extirpation. Postoperative radiation therapy was used for malignant lesions. Patient outcomes are delineated. Conclusion: Neoplasms, both benign and malignant, of the