- Email: [email protected]
Determination of genetic diversity of native Bombus terrestris (Hymenoptera: Apidae) populations in the Mediterranean Region by microsatellite markers
Current Opinion in Biotechnology 24S (2013) S48–S143
Contents lists available at ScienceDirect
Current Opinion in Biotechnology
Poster presentations
Animal Biotechnology
Transcription silencing in porcine oocytes
Determination of genetic diversity of native Bombus terrestris (Hymenoptera: Apidae) populations in the Mediterranean Region by microsatellite markers
Frantisek Strejcek 1 , Martin Morovic 1 , Irena Barnetova 4 , Olga Østrup 2 , Poul Hyttel 2 , Heiner Niemann 3 , Josef Fulka Jr. 4 , Helena Fulka 4 , Jozef Laurincik 1
Bahar Argun Karsli 1 , Fehmi Gurel 1 , Hasan Meydan 2 , Mehmet Ali Yildiz 2
Constantine the Philosopher University, Nitra, Slovak Republic Department of Basic Animal and Veterinary Sciences, Faculty of Life Sciences, University of Copenhagen, Copenhagen, Denmark 3 Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut, Neustadt, Germany 4 Department of Biology of Reproduction, Institute of Animal Science, Prague, Czech Republic E-mail address: [email protected] (F. Strejcek).
1
Department of Animal Science, Faculty of Agriculture Antalya, Akdeniz University, Turkey 2 Department of Animal Science, Faculty of Agriculture, Ankara Ankara University, Turkey E-mail address: [email protected] (F. Gurel). A total of 107 worker bee samples were collected from 3 native Bombus terrestris populations (Antalya-Kemer, Antalya-Termesos and Adana) in the Mediterranean Region of Turkey. The genetic structure of the native B. terrestris populations was investigated by using ten microsatellite markers (BT06, BT09, BT20, B100, B116, B118, B119, B121, B124 and B126). The mean number of alleles (Na), effective number of alleles (Ne), observed heterozygosity (Ho) and expected heterozygosity (He) values for the AntalyaKemer, Antalya-Termesos and Adana populations were estimated as Na = 10.3, 9.8 and 9.2, Ne = 6.4, 5.7 and 5.8, Ho = 0.73, 0.71 and 0.70 and He = 0.75, 0.72 and 0.74 respectively. There was not significant deviation from Hardy–Weinberg equilibrium in the population. Genetic data generated in the present study showed that examined microsatellite loci were not effective markers to distinguish of B. terrestris ecotypes. http://dx.doi.org/10.1016/j.copbio.2013.05.110
1
2
Global transcription silencing occurs in the oocyte during its final phase of growth. The particular mechanism of this silencing is not well understood. We investigated the transcriptional activity of fully grown germinal vesicle oocytes maturated: in vivo (derived from stimulated — 6 hours after hCG treatment and nonstimulated gilts — before hCG treatment) or in vitro (after 30 min or 6 hours cultivation). Oocytes were incubated with radioactive precursors (3H-uridine, 3H-adenosine) for 20 min to 6 hours to detect the accumulation of slowly labelled RNA. Among the test groups, there was no transcription detected in in vivo matured oocytes, and almost no transcription was detected in in vitromatured oocytes. As we did not observe any incorporation of the precursors in our autoradiography studies, we concluded that there is no active transcription in GV oocytes, and also we thus concluded that gonadotropins did not affect the oocyte transcriptional activity. The discrepancy of autoradiography labelling with another observation still remains to be elucidated. Acknowledgement: This work was supported by VEGA 1/0077/11 and GACR P302/11/P069. http://dx.doi.org/10.1016/j.copbio.2013.05.111
0958-1669/$ – see front matter http://dx.doi.org/10.1016/j.copbio.2013.05.017
Contents lists available at ScienceDirect
Current Opinion in Biotechnology
Poster presentations
Animal Biotechnology
Transcription silencing in porcine oocytes
Determination of genetic diversity of native Bombus terrestris (Hymenoptera: Apidae) populations in the Mediterranean Region by microsatellite markers
Frantisek Strejcek 1 , Martin Morovic 1 , Irena Barnetova 4 , Olga Østrup 2 , Poul Hyttel 2 , Heiner Niemann 3 , Josef Fulka Jr. 4 , Helena Fulka 4 , Jozef Laurincik 1
Bahar Argun Karsli 1 , Fehmi Gurel 1 , Hasan Meydan 2 , Mehmet Ali Yildiz 2
Constantine the Philosopher University, Nitra, Slovak Republic Department of Basic Animal and Veterinary Sciences, Faculty of Life Sciences, University of Copenhagen, Copenhagen, Denmark 3 Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut, Neustadt, Germany 4 Department of Biology of Reproduction, Institute of Animal Science, Prague, Czech Republic E-mail address: [email protected] (F. Strejcek).
1
Department of Animal Science, Faculty of Agriculture Antalya, Akdeniz University, Turkey 2 Department of Animal Science, Faculty of Agriculture, Ankara Ankara University, Turkey E-mail address: [email protected] (F. Gurel). A total of 107 worker bee samples were collected from 3 native Bombus terrestris populations (Antalya-Kemer, Antalya-Termesos and Adana) in the Mediterranean Region of Turkey. The genetic structure of the native B. terrestris populations was investigated by using ten microsatellite markers (BT06, BT09, BT20, B100, B116, B118, B119, B121, B124 and B126). The mean number of alleles (Na), effective number of alleles (Ne), observed heterozygosity (Ho) and expected heterozygosity (He) values for the AntalyaKemer, Antalya-Termesos and Adana populations were estimated as Na = 10.3, 9.8 and 9.2, Ne = 6.4, 5.7 and 5.8, Ho = 0.73, 0.71 and 0.70 and He = 0.75, 0.72 and 0.74 respectively. There was not significant deviation from Hardy–Weinberg equilibrium in the population. Genetic data generated in the present study showed that examined microsatellite loci were not effective markers to distinguish of B. terrestris ecotypes. http://dx.doi.org/10.1016/j.copbio.2013.05.110
1
2
Global transcription silencing occurs in the oocyte during its final phase of growth. The particular mechanism of this silencing is not well understood. We investigated the transcriptional activity of fully grown germinal vesicle oocytes maturated: in vivo (derived from stimulated — 6 hours after hCG treatment and nonstimulated gilts — before hCG treatment) or in vitro (after 30 min or 6 hours cultivation). Oocytes were incubated with radioactive precursors (3H-uridine, 3H-adenosine) for 20 min to 6 hours to detect the accumulation of slowly labelled RNA. Among the test groups, there was no transcription detected in in vivo matured oocytes, and almost no transcription was detected in in vitromatured oocytes. As we did not observe any incorporation of the precursors in our autoradiography studies, we concluded that there is no active transcription in GV oocytes, and also we thus concluded that gonadotropins did not affect the oocyte transcriptional activity. The discrepancy of autoradiography labelling with another observation still remains to be elucidated. Acknowledgement: This work was supported by VEGA 1/0077/11 and GACR P302/11/P069. http://dx.doi.org/10.1016/j.copbio.2013.05.111
0958-1669/$ – see front matter http://dx.doi.org/10.1016/j.copbio.2013.05.017