- Email: [email protected]
Determination of NS3 inhibitors drug resistance mutations in chronic Hepatitis C patients infected with genotype 1
S84
Abstracts / Journal of Biotechnology 256S (2017) S44–S116
The above processes occurring in the lipidic bilayer of erythrocytic membranes cause changes of cytoarchitectonics, conformation of hemoglobin and, consequently, affect the basic function of erythrocytes – transport of oxygen. This mechanism is probably one of the most important parts in the development of ischemic heart disease. This work was supported by the Russian Science Foundation, Grant No. 15-15-10025. http://dx.doi.org/10.1016/j.jbiotec.2017.06.1086 Determination of NS3 inhibitors drug resistance mutations in chronic Hepatitis C patients infected with genotype 1 Tamer Sanlıdag 1 , Tamer Sanlidag 2 , Murat Sayan 2 , ˙ Murat Sayan 3 , Sinem Akcali 1 , Elmas Kasap 4 , 4 Tahir˙ Buran , Ayse Arikan 5,∗ , Ayse Arikan 6,∗ 1 Celal Bayar University Faculty of Medicine, Department of Medical Microbiology, Manisa, Turkey 2 Near East University, Experimental Health Sciences Research Center, Nicosia, Cyprus 3 Kocaeli University Research and Practice Hospital, Kocaeli, Turkey 4 Celal Bayar University Faculty of Medicine, Department of Internal Medicine, Manisa, Turkey 5 Near East University Faculty of Medicine, Department of Medical Microbiology, Nicosia, Cyprus 6 Kyrenia University Faculty of Medicine, Department of Medical Microbiology, Kyrenia, Cyprus
E-mail address: [email protected] (A. Arikan). NS3 inhibitors are used for chronic hepatitis C (CHC) treatment although naturally occurring resistant variants can emerge. In this study, it was aimed to investigate new and old generation NS3 inhibitors resistance mutations. Treated naive 97 patients infected with hepatitis c virus (HCV) genotype 1 were analyzed. Magnetic particle based HCV RNA extraction, detection, quantification was performed using commercial real-time PCR assay QIASypmhony + Rotorgene Q/artus HCV QS-RGQ and COBAS Ampliprep/COBAS TaqMan HCV Test. NS3 viral protease genome region was amplified through PCR, mutation analysis was performed by Sanger dideoxy sequencing technique of NS3 protease codons. Asunaprevir, boceprevir, faldaprevir, grazoprevir, pariteprevir, simeprevir, telaprevir were analyzed for resistance mutation by Geno2pheno-HCV resistance tool. Eighty-eight patients had genotype 1. Eight and 80 patients had 1a and 1b subgenotypes respectively. Thirty-nine patients had amino acid substitutions and resistance mutations. Six had potential substitutions (Q80L, S122C/N, S138W); 34 had potential resistance (R109K, R117C, S122G, I132V, I170V, N174S); 7 had resistance (V36L, T54S, V55A, Q80H); 3 had high resistance mutations (Q80K, A156S). Based on resistant and high-resistance mutations, clinically significant mutations were identified in 10 patients. NS3 resistance analysis is required before treatment. Analysis of NS5A and NS5B genome region mutations may also be required in the near future. http://dx.doi.org/10.1016/j.jbiotec.2017.06.1087
Blood compatibility studies of silk fibroin-polyethylene glycol dimethacrylate microfibers Fatma Zor 1 , Rasime Kalkan 2 , Eda Becer 3,∗ , Tamer Sanlıdag 4 , Terin Adalı 1 1 Department of Biomedical Engineering, Near East University, Nicosia, Cyprus 2 Department of Medical Genetics, Faculty of Medicine, Near East University, Nicosia, Cyprus 3 Department of Biochemistry, Faculty of Pharmacy, Near East University, Nicosia, Cyprus 4 Research Center of Experimental Health Sciences, Near East University, Nicosia, Cyprus
E-mail address: [email protected] (E. Becer). Silk fibroin (SF), is a versatile protein-polymer, being synthesized into varitey of formats to match physical, chemical structural and morphological features for specific biomedical applications. The main aim of this study was to synthesize, characterize and investigate blood compatibility of polyethylene glycol dimethacrylate coated silk fibroin microfibers. Layer-by-layer method has been used for coating polyethylene glycol dimethacrylate on silk fibroin microfibers at 60 ◦ C and 1 rpm. The average size of four twisted microfibers after coating was reported as 559.96 m and 19.5% coating with polyethylene glycol dimethcrylate has been successfully achieved. The microfilms were not biodegradable with protease enzyme. Smooth surface coating has been observed on the SEM micrographs. The anticoagulation activity was evaluated using prothrombin time (PT), activated partial thromboplastin time (APTT) at different time intervals. The in vitro coagulation tests indicated that polyethylene glycol dimethacrylate coated silk fibroin microfibers exhibited better hemocompatibility in comparison with pure silk fibroin microfibers. These results showed that the silk fibroin - polyethylene glycol dimethacrylate (SF-PEGDMA) microfibers have potential applications as blood contact fabrics and patches. http://dx.doi.org/10.1016/j.jbiotec.2017.06.1088 “In vitro” testing of the antimicrobial action of certain hydroalcoholic plant extracts to control major bacterial diseases in bees Savu Vasilic˘a 1,∗ , Sapcaliu Agripina 1 , Negoita Carmen 2 , R˘adoi Ion 2 , T˘anase Petrut¸ 3 , Pîrvulet¸ Cristina Stefania 4 1
Beekeeping Research and Development Institute Bucharest, Romania 2 University of Agronomical Sciences and Veterinary Medicine Bucharest, Romania 3 Spiru Haret University Bucharest, Romania 4 Academy of Agricultural and Forestry Sciences “Gheorghe Ionescu-Sisesti”, Romania E-mail address: [email protected] (S. Vasilic˘a). For the purpose of obtaining an apiphytotherapeutic formula to control major bacterial diseases in bees (American foulbrood) 10 plants in Romania’s flora were evaluated for their antimicrobial activity. To establish the antimicrobial activity, 6 hydroalcoholic plant extracts (Ocimum basilicum, Melissa officinalis, Thymus serpyllum, Rosmarinum officinale, Artemisia absinthium, Origanum vulgare) were selected, according to the content of essential (volatile) oils, flavonoids and polyphenolic compounds, as well as the propolis extract. The “in vitro” testing of the antimicrobial activity was
Abstracts / Journal of Biotechnology 256S (2017) S44–S116
The above processes occurring in the lipidic bilayer of erythrocytic membranes cause changes of cytoarchitectonics, conformation of hemoglobin and, consequently, affect the basic function of erythrocytes – transport of oxygen. This mechanism is probably one of the most important parts in the development of ischemic heart disease. This work was supported by the Russian Science Foundation, Grant No. 15-15-10025. http://dx.doi.org/10.1016/j.jbiotec.2017.06.1086 Determination of NS3 inhibitors drug resistance mutations in chronic Hepatitis C patients infected with genotype 1 Tamer Sanlıdag 1 , Tamer Sanlidag 2 , Murat Sayan 2 , ˙ Murat Sayan 3 , Sinem Akcali 1 , Elmas Kasap 4 , 4 Tahir˙ Buran , Ayse Arikan 5,∗ , Ayse Arikan 6,∗ 1 Celal Bayar University Faculty of Medicine, Department of Medical Microbiology, Manisa, Turkey 2 Near East University, Experimental Health Sciences Research Center, Nicosia, Cyprus 3 Kocaeli University Research and Practice Hospital, Kocaeli, Turkey 4 Celal Bayar University Faculty of Medicine, Department of Internal Medicine, Manisa, Turkey 5 Near East University Faculty of Medicine, Department of Medical Microbiology, Nicosia, Cyprus 6 Kyrenia University Faculty of Medicine, Department of Medical Microbiology, Kyrenia, Cyprus
E-mail address: [email protected] (A. Arikan). NS3 inhibitors are used for chronic hepatitis C (CHC) treatment although naturally occurring resistant variants can emerge. In this study, it was aimed to investigate new and old generation NS3 inhibitors resistance mutations. Treated naive 97 patients infected with hepatitis c virus (HCV) genotype 1 were analyzed. Magnetic particle based HCV RNA extraction, detection, quantification was performed using commercial real-time PCR assay QIASypmhony + Rotorgene Q/artus HCV QS-RGQ and COBAS Ampliprep/COBAS TaqMan HCV Test. NS3 viral protease genome region was amplified through PCR, mutation analysis was performed by Sanger dideoxy sequencing technique of NS3 protease codons. Asunaprevir, boceprevir, faldaprevir, grazoprevir, pariteprevir, simeprevir, telaprevir were analyzed for resistance mutation by Geno2pheno-HCV resistance tool. Eighty-eight patients had genotype 1. Eight and 80 patients had 1a and 1b subgenotypes respectively. Thirty-nine patients had amino acid substitutions and resistance mutations. Six had potential substitutions (Q80L, S122C/N, S138W); 34 had potential resistance (R109K, R117C, S122G, I132V, I170V, N174S); 7 had resistance (V36L, T54S, V55A, Q80H); 3 had high resistance mutations (Q80K, A156S). Based on resistant and high-resistance mutations, clinically significant mutations were identified in 10 patients. NS3 resistance analysis is required before treatment. Analysis of NS5A and NS5B genome region mutations may also be required in the near future. http://dx.doi.org/10.1016/j.jbiotec.2017.06.1087
Blood compatibility studies of silk fibroin-polyethylene glycol dimethacrylate microfibers Fatma Zor 1 , Rasime Kalkan 2 , Eda Becer 3,∗ , Tamer Sanlıdag 4 , Terin Adalı 1 1 Department of Biomedical Engineering, Near East University, Nicosia, Cyprus 2 Department of Medical Genetics, Faculty of Medicine, Near East University, Nicosia, Cyprus 3 Department of Biochemistry, Faculty of Pharmacy, Near East University, Nicosia, Cyprus 4 Research Center of Experimental Health Sciences, Near East University, Nicosia, Cyprus
E-mail address: [email protected] (E. Becer). Silk fibroin (SF), is a versatile protein-polymer, being synthesized into varitey of formats to match physical, chemical structural and morphological features for specific biomedical applications. The main aim of this study was to synthesize, characterize and investigate blood compatibility of polyethylene glycol dimethacrylate coated silk fibroin microfibers. Layer-by-layer method has been used for coating polyethylene glycol dimethacrylate on silk fibroin microfibers at 60 ◦ C and 1 rpm. The average size of four twisted microfibers after coating was reported as 559.96 m and 19.5% coating with polyethylene glycol dimethcrylate has been successfully achieved. The microfilms were not biodegradable with protease enzyme. Smooth surface coating has been observed on the SEM micrographs. The anticoagulation activity was evaluated using prothrombin time (PT), activated partial thromboplastin time (APTT) at different time intervals. The in vitro coagulation tests indicated that polyethylene glycol dimethacrylate coated silk fibroin microfibers exhibited better hemocompatibility in comparison with pure silk fibroin microfibers. These results showed that the silk fibroin - polyethylene glycol dimethacrylate (SF-PEGDMA) microfibers have potential applications as blood contact fabrics and patches. http://dx.doi.org/10.1016/j.jbiotec.2017.06.1088 “In vitro” testing of the antimicrobial action of certain hydroalcoholic plant extracts to control major bacterial diseases in bees Savu Vasilic˘a 1,∗ , Sapcaliu Agripina 1 , Negoita Carmen 2 , R˘adoi Ion 2 , T˘anase Petrut¸ 3 , Pîrvulet¸ Cristina Stefania 4 1
Beekeeping Research and Development Institute Bucharest, Romania 2 University of Agronomical Sciences and Veterinary Medicine Bucharest, Romania 3 Spiru Haret University Bucharest, Romania 4 Academy of Agricultural and Forestry Sciences “Gheorghe Ionescu-Sisesti”, Romania E-mail address: [email protected] (S. Vasilic˘a). For the purpose of obtaining an apiphytotherapeutic formula to control major bacterial diseases in bees (American foulbrood) 10 plants in Romania’s flora were evaluated for their antimicrobial activity. To establish the antimicrobial activity, 6 hydroalcoholic plant extracts (Ocimum basilicum, Melissa officinalis, Thymus serpyllum, Rosmarinum officinale, Artemisia absinthium, Origanum vulgare) were selected, according to the content of essential (volatile) oils, flavonoids and polyphenolic compounds, as well as the propolis extract. The “in vitro” testing of the antimicrobial activity was