- Email: [email protected]
Determination of serum albumin on gradiation of fatty liver diseases
Abstracts
Introduction: Cardiac syndrome X (CSX) includes chest pain and positive exercise and/or radionuclide test and normal coronary angiography. Previous studies have suggested that Helicobacter pylori (H. pylori) infection and endothelial function may play important roles in the pathogenesis of CSX. We studied association of H.pylori infection and endothelial function in cardiac syndrome X. Methods: The study population comprised 80 selected patients (30 males/50 females, mean age: 51.71 ± 9.2) who had been diagnosed as CSX. Patients were divided into two groups according to the presence or absence of anti-H. pylori IgG antibody. Also, plasma levels of intercellular adhesion molecule-1 (ICAM-1), as an endothelial function marker, were measured using ELISA method. Results: Fifty six anti-H. pylori (+) patients (23 males/33 females; mean age: 51.25 ± 8.86) were compared with 24 anti-H. pylori (−) patients (7 males/17 females; mean age: 52.79 ± 0.88) (p > 0.05). The levels of ICAM-1 were significantly higher in H. pylori (+) than H. pylori (−) patients (349.62± 20.23 vs 266.51 ± 25.54 ng/ml, respectively; (P< 0.05). Conclusion: This study showed that there is an association between H. pylori infection and ICAM-1 as an endothelial function marker. This data suggest that H. pylori infection might contribute to the development of CSX via endothelial dysfunction. Keywords: Cardiac syndrome X, Endothelial function, Helicobacter pylori, Intercellular adhesion molecule-1 doi:10.1016/j.clinbiochem.2011.08.094
E Poster – [A-10-335-1] Toxicity effects of hydroxy coumarin derivations on induction of apoptosis in MCF7 cell line Golahmadi Sanaza, Mohammad Soukhtanloob, Hamid Sadeghianc, Javad Sargolzaeia a Department of Biology, School of Basic Sciences, Payam nor University, Mashhad, Iran b Department of Clinical Biochemistry, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran c Department of Laboratory Science, School of Paramedical science, Mashhad University of Medical Sciences, Mashhad, Iran E-mail addresses: [email protected] (G. Sanaz), [email protected] (M. Soukhtanloo), [email protected] (H. Sadeghian), [email protected] (J. Sargolzaei) Introduction: Breast cancer, the most commonly diagnosed cancer among women, is the leading cause of death, resulting from the metastatic spread of primary tumors. In this study, we studied the toxicity effect of some o-prenylated coumarins on cellular class of MCF-7. Methods: MCF-7 in RPMI medium with bovine fetal serum and antibiotic were cultured, in the presense of different concentrations of drive prenyl hydroxy coumarin during 48 h. Cell viability was quantitated by MTT assay in order to determine dose and apoptotic cell was determined using PI staining of DNA fragmentation by flow cytometry (sub G1 peak). Result: 50 μm/ml 6-farnesyl oxi coumarin induces the apoptosis on the cells. Toxicity effect of 6-farnesyl in MCF-7 cell starts at 25 μm/ml concentration and increases relatively with concentration and time. This compound induces apoptosis in MCF-7 cells in 50 μm/ml and more, toxicity and apoptosis in medicines such as 6-farnesil oxi coumarin and 3farnesil oxi coumarin and 3-geranil oxi coumarin is more than 7-farnesil oxi coumarin and 6-geranil oxi coumarin in intomoric cells apoptosis and toxicity haven't seen in any time and concentration. Conclusion: In conclusion 3-farnesil oxi coumarin and 6-farnesil oxi coumarin and 3-geranil oxi coumarin could be considered as a promising chemotherapeutic agent in breast cancer treatment in the future.
S47
Keywords: Cancer, Apoptosis, Hydroxy coumarin derivations, Flow cytometry, MCF7 cell line doi:10.1016/j.clinbiochem.2011.08.095
E Poster – [A-10-348-1] Effects of melatonin on plasma and lenticular oxidative stress in rats with streptozotocin induced diabetic cataract Masoumeh Akmalia, Mojtaba Beheshtitabarb, Marjan Khorsandb a Department of Biochemistry, Shiraz University of Medical Sciences, Shiraz, Iran b Department of Biochemistry, Yazd Shahid Sadoughi University of Medical Sciences, Yazd, Iran E-mail addresses: [email protected] (M. Akmali), [email protected] (M. Beheshtitabar), [email protected] (M. Khorsand) Introduction: Melatonin (Mel) is a well known powerful antioxidant. The effects of oral administration of melatonin against streptozotocin (STZ)-induced diabetic cataract in rat were investigated. Materials and methods: Thirty adult male Sprague–Dawely rats were divided into three equal groups: control, diabetic model, and melatonin treated groups. The diabetic model and Mel treatment rats were induced to diabetes by intraperitonal injection with STZ (50 mg/kg B.W.). The melatonin groups were administrated Mel (5 mg/kg B.W.) once a day for 8 weeks. The control and diabetic group's rats also received the vehicle. Cataract progress was monitored using a slit lamp biomicroscope. Blood glucose levels were measured every two weeks up to the 8 weeks. After 8 weeks, all rats were killed and blood samples were collected from the heart. The lenses were dissected immediately. Malondialdehyde (MDA) and reduced glutathione (GSH) levels were measured in plasma and lenses homogenate. The activities of catalase (CAT) and glutathione reductase (GR) were also determined in rat's lenses. Results and conclusion: The specific activities of CAT and GR and GSH content decreased significantly in diabetic rats. STZ also significantly increased the MDA level, as an end product of lipid peroxidation. Melatonin administration increased specific activity of CAT and GR and GSH content in diabetic rat lenses (p <0.05). Diabetes resulted in increased plasma MDA levels and decreased plasma GSH levels whereas melatonin reverse these changes in plasma (p <0.05). Conclusion: Melatonin can inhibit the progression of the diabetic cataract by increasing of GSH content and antioxidant enzymes activities such as GR and CAT in lens. Keywords: Diabetic cataract, Melatonin, Antioxidant enzymes, Malondialdehyde, Streptozotocin doi:10.1016/j.clinbiochem.2011.08.096
E Poster – [A-10-349-1] Determination of serum albumin on gradiation of fatty liver diseases Ahmadpur Ghorbana, Qujeq Durdib, Abedin Saeidc, Hossini Vahidc a Dept. of Biology, Research and Science unit, Tehran Azad University, Iran b Dept. of Biochemistry Babol University of Medical Sciences, Babol, Iran c Faculty of Medicine, Mazandaran Uuiversity of Medical Sciences, Iran E-mail addresses: [email protected] (A. Ghorban), [email protected] (Q. Durdi) Introduction: Nonalcoholic fatty liver disease is a fatty liver disease occurring in patients without alcohol consumption and encompasses a spectrum of liver disease from simple steatosis without significant necroinflammation to varying degrees of steatohepatitis and fibrosis.
S48
Abstracts
Steatosis was graded as mild, moderate or severe. Human serum albumin synthesized in the liver and binds fatty acids. Oxidative stress can modify albumin and affect ligand binding. Materials and methods: Subjects with normal liver (30) and fatty liver sonogram (30) were randomly collected. Anthropometric parameters, fasting glucose, alaninaminotransferase (ALT) and albumin concentrations were measured in each subject. Results: Blood pressure was significantly higher, while fasting glucose was significantly lower in patients with fatty liver.The mean ALT were 66.29 ± 77.74, 40.00 ± 32.86 and 36.00 ± 4.00, respectively in mild, moderate and severe fatty liver patients and 22.45± 5.25 (IU/L) in control (p= 0.001). The mean albumin concentrations were 4.72 ± 0.23 (g/dL), 4.82± 0.27 (g/dL), 4.36 ± 0.15 (g/dL), respectively in mild, moderate and severe in fatty liver and 4.63 ± 0.26 (g/dL) in control group (p= 0.56). Conclusions: Blood pressure and AST activity were significantly higher, but blood sugar was significantly lower in fatty liver. Severity of steatosis and albumin concenteration is statistically significant. Keywords: Fatty liver, Steatosis, Albumin doi:10.1016/j.clinbiochem.2011.08.097
E Poster – [A-10-401-2] Polyethersulfone membranes for cell culture systems Azadbakht Mehria, Madaeni Sayed Siavashb, Sahebjamee Foroodb a Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran b Membrane Research Center, Department of Chemical Engineering, Razi University, Kermanshah, Iran E-mail addresses: [email protected] (A. Mehri), [email protected] (M.S. Siavash), [email protected] (S. Forood)
E Poster – [A-10-411-1] Lowering BNP cut-points is beneficial in obese patients diagnostic Opris Simona, Constantin Gianina Caldarusani 9, Bucharest, Romania E-mail addresses: [email protected] (O. Simona), [email protected] (C. Gianina) Introduction: Physiologically, brain natriuretic peptides (BNP) and lipolysis are closely linked. Obesity has been identified as a major risk factor for the development of cardiovascular diseases (CVD) and has been reported to have an impact on BNP in apparently healthy subjects but also in CVD patients. Thus, we speculate that BNP could play an important role in lipid metabolism and may affect the pathophysiology of obesity in CVD patients. Materials and methods: Serum samples were obtained from CVD elderly patients distributed in 2 groups: I-non-obese and II-obese. The plasma mature form of brain natriuretic peptide (NT-proBNP) was measured by a sandwich enzyme immunoassay with spectrophotometric detection at 450 nm. Results: Our study revealed for group I with BMI (22.77 ± 2.15) higher BNP levels (18.79 ± 16.87 pmol/L) versus group II (16.57 ± 20.81 pmol/L) with BMI 33.33 ± 4.75. Thereby, despite a similar severity of CVD, levels of BNP were 11.81% lower in obese than in non-obese patients. Conclusion: Overall, these data demonstrates that obesity is an important and independent determinant of BNP expression in patients with CVD. Inverse relationship between BNP and body mass index may suggests “beneficial” effects of obesity, but clearly lower levels did not confer a more favourable prognosis. The precise mechanisms linking obesity to CVD remain unsolved and may be due either to release attenuation or increases in clearance receptors. These effects should be taken into account for appropriate BNP reference values, so lower cut-points should be used for obese patients and a higher cut-point for lean patients to increase specificity. Keywords: Natriuretic peptides, Obesity, Cardiovascular diseases
Introduction: Polyethersulfone (PES) membranes with high chemical, thermal and mechanical stability can be used for low-cost membrane preparation. In this study, PES membranes and cell culture inserts were prepared and optimized for utilization in cell culture systems. Materials and methods: Polyethersulfone membranes were prepared using phase inversion via immersion precipitation. The film was moved to the non-solvent bath, a mixture of water and ethanol for precipitation. The membrane was stored in distilled water for 24 h. The performances of non-transparent, transparent and commercial PES membranes were compared. The structures of the membranes were observed by inverted optical microscope. The cyto-compatibility of PES membrane was evaluated by culturing HepG2, a human liver (carcinoma) cell line. Cell viability and adhesion were evaluated. The biological activity of cells was determined by uptake of Indocyanine Green (ICG), and Periodic Acid Schiff (PAS) staining. Results: Although the morphology of non-transparent and transparent PES membranes was different from commercial membrane, they demonstrated similar performances. The cells showed round or oval shape and adhered to form three-dimensional aggregates on the membrane. Cellular uptake of ICG was detected in cells and glycogen storage was determined by PAS staining methods. Conclusion: The activity of the cells cultured on membrane confirmed the acceptable properties of the prepared membranes. The homemade cell culture inserts showed satisfactory performance compared to commercial ones. Keywords: Membrane, Polyethersulfone, Cell culture, HepG2 cell line doi:10.1016/j.clinbiochem.2011.08.098
doi:10.1016/j.clinbiochem.2011.08.099
E Poster – [A-10-427-1] Glucose-6-phosphate dehydrogenase deficiency in the newborn in relation to neonatal hyperbilirubinaemia Mehrotra Vinita, Sharma Anitab, M. Tiwaric a Biochemistry, HIHT, Jollygrant, Doiwala, Dehradun, India b Medicine, HIH, Jollygrant, Doiwala, Dehradun, India c Medicine, HIHT, Jollygrant, Doiwala, Dehradun, India E-mail addresses: [email protected] (M. Vinit), [email protected] (S. Anita), [email protected] (M. Tiwari) Introduction: Hyperbilirubinaemia or jaundice is a common problem in newborn infants. Glucose-6-phosphate dehydrogenase (G6PD) deficiency can cause acute hemolysis during oxidative stress and also severe hyperbilirubinaemia in the newborn. Our aim was to study the prevalence of G6PD deficiency in relation to neonatal jaundice. Materials and methods: We performed G6PD assay in the samples of 280 subjects. Observation of jaundice and determination of bilirubin level as well as work up for other causes of jaundice were made in the G6PD deficiency group compared to a G6PD normal group. 160 were clinically jaundiced and only 106 were included in the study whereas rests were excluded due to presence of direct hyperbilirubinaemia. Results: Neonatal jaundice was found to be affecting 57.14% with male to female ratio of 2.4:1 and common cause was physiological jaundice 42.45%. It was more severe in cases due to Rh incompatibility
Introduction: Cardiac syndrome X (CSX) includes chest pain and positive exercise and/or radionuclide test and normal coronary angiography. Previous studies have suggested that Helicobacter pylori (H. pylori) infection and endothelial function may play important roles in the pathogenesis of CSX. We studied association of H.pylori infection and endothelial function in cardiac syndrome X. Methods: The study population comprised 80 selected patients (30 males/50 females, mean age: 51.71 ± 9.2) who had been diagnosed as CSX. Patients were divided into two groups according to the presence or absence of anti-H. pylori IgG antibody. Also, plasma levels of intercellular adhesion molecule-1 (ICAM-1), as an endothelial function marker, were measured using ELISA method. Results: Fifty six anti-H. pylori (+) patients (23 males/33 females; mean age: 51.25 ± 8.86) were compared with 24 anti-H. pylori (−) patients (7 males/17 females; mean age: 52.79 ± 0.88) (p > 0.05). The levels of ICAM-1 were significantly higher in H. pylori (+) than H. pylori (−) patients (349.62± 20.23 vs 266.51 ± 25.54 ng/ml, respectively; (P< 0.05). Conclusion: This study showed that there is an association between H. pylori infection and ICAM-1 as an endothelial function marker. This data suggest that H. pylori infection might contribute to the development of CSX via endothelial dysfunction. Keywords: Cardiac syndrome X, Endothelial function, Helicobacter pylori, Intercellular adhesion molecule-1 doi:10.1016/j.clinbiochem.2011.08.094
E Poster – [A-10-335-1] Toxicity effects of hydroxy coumarin derivations on induction of apoptosis in MCF7 cell line Golahmadi Sanaza, Mohammad Soukhtanloob, Hamid Sadeghianc, Javad Sargolzaeia a Department of Biology, School of Basic Sciences, Payam nor University, Mashhad, Iran b Department of Clinical Biochemistry, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran c Department of Laboratory Science, School of Paramedical science, Mashhad University of Medical Sciences, Mashhad, Iran E-mail addresses: [email protected] (G. Sanaz), [email protected] (M. Soukhtanloo), [email protected] (H. Sadeghian), [email protected] (J. Sargolzaei) Introduction: Breast cancer, the most commonly diagnosed cancer among women, is the leading cause of death, resulting from the metastatic spread of primary tumors. In this study, we studied the toxicity effect of some o-prenylated coumarins on cellular class of MCF-7. Methods: MCF-7 in RPMI medium with bovine fetal serum and antibiotic were cultured, in the presense of different concentrations of drive prenyl hydroxy coumarin during 48 h. Cell viability was quantitated by MTT assay in order to determine dose and apoptotic cell was determined using PI staining of DNA fragmentation by flow cytometry (sub G1 peak). Result: 50 μm/ml 6-farnesyl oxi coumarin induces the apoptosis on the cells. Toxicity effect of 6-farnesyl in MCF-7 cell starts at 25 μm/ml concentration and increases relatively with concentration and time. This compound induces apoptosis in MCF-7 cells in 50 μm/ml and more, toxicity and apoptosis in medicines such as 6-farnesil oxi coumarin and 3farnesil oxi coumarin and 3-geranil oxi coumarin is more than 7-farnesil oxi coumarin and 6-geranil oxi coumarin in intomoric cells apoptosis and toxicity haven't seen in any time and concentration. Conclusion: In conclusion 3-farnesil oxi coumarin and 6-farnesil oxi coumarin and 3-geranil oxi coumarin could be considered as a promising chemotherapeutic agent in breast cancer treatment in the future.
S47
Keywords: Cancer, Apoptosis, Hydroxy coumarin derivations, Flow cytometry, MCF7 cell line doi:10.1016/j.clinbiochem.2011.08.095
E Poster – [A-10-348-1] Effects of melatonin on plasma and lenticular oxidative stress in rats with streptozotocin induced diabetic cataract Masoumeh Akmalia, Mojtaba Beheshtitabarb, Marjan Khorsandb a Department of Biochemistry, Shiraz University of Medical Sciences, Shiraz, Iran b Department of Biochemistry, Yazd Shahid Sadoughi University of Medical Sciences, Yazd, Iran E-mail addresses: [email protected] (M. Akmali), [email protected] (M. Beheshtitabar), [email protected] (M. Khorsand) Introduction: Melatonin (Mel) is a well known powerful antioxidant. The effects of oral administration of melatonin against streptozotocin (STZ)-induced diabetic cataract in rat were investigated. Materials and methods: Thirty adult male Sprague–Dawely rats were divided into three equal groups: control, diabetic model, and melatonin treated groups. The diabetic model and Mel treatment rats were induced to diabetes by intraperitonal injection with STZ (50 mg/kg B.W.). The melatonin groups were administrated Mel (5 mg/kg B.W.) once a day for 8 weeks. The control and diabetic group's rats also received the vehicle. Cataract progress was monitored using a slit lamp biomicroscope. Blood glucose levels were measured every two weeks up to the 8 weeks. After 8 weeks, all rats were killed and blood samples were collected from the heart. The lenses were dissected immediately. Malondialdehyde (MDA) and reduced glutathione (GSH) levels were measured in plasma and lenses homogenate. The activities of catalase (CAT) and glutathione reductase (GR) were also determined in rat's lenses. Results and conclusion: The specific activities of CAT and GR and GSH content decreased significantly in diabetic rats. STZ also significantly increased the MDA level, as an end product of lipid peroxidation. Melatonin administration increased specific activity of CAT and GR and GSH content in diabetic rat lenses (p <0.05). Diabetes resulted in increased plasma MDA levels and decreased plasma GSH levels whereas melatonin reverse these changes in plasma (p <0.05). Conclusion: Melatonin can inhibit the progression of the diabetic cataract by increasing of GSH content and antioxidant enzymes activities such as GR and CAT in lens. Keywords: Diabetic cataract, Melatonin, Antioxidant enzymes, Malondialdehyde, Streptozotocin doi:10.1016/j.clinbiochem.2011.08.096
E Poster – [A-10-349-1] Determination of serum albumin on gradiation of fatty liver diseases Ahmadpur Ghorbana, Qujeq Durdib, Abedin Saeidc, Hossini Vahidc a Dept. of Biology, Research and Science unit, Tehran Azad University, Iran b Dept. of Biochemistry Babol University of Medical Sciences, Babol, Iran c Faculty of Medicine, Mazandaran Uuiversity of Medical Sciences, Iran E-mail addresses: [email protected] (A. Ghorban), [email protected] (Q. Durdi) Introduction: Nonalcoholic fatty liver disease is a fatty liver disease occurring in patients without alcohol consumption and encompasses a spectrum of liver disease from simple steatosis without significant necroinflammation to varying degrees of steatohepatitis and fibrosis.
S48
Abstracts
Steatosis was graded as mild, moderate or severe. Human serum albumin synthesized in the liver and binds fatty acids. Oxidative stress can modify albumin and affect ligand binding. Materials and methods: Subjects with normal liver (30) and fatty liver sonogram (30) were randomly collected. Anthropometric parameters, fasting glucose, alaninaminotransferase (ALT) and albumin concentrations were measured in each subject. Results: Blood pressure was significantly higher, while fasting glucose was significantly lower in patients with fatty liver.The mean ALT were 66.29 ± 77.74, 40.00 ± 32.86 and 36.00 ± 4.00, respectively in mild, moderate and severe fatty liver patients and 22.45± 5.25 (IU/L) in control (p= 0.001). The mean albumin concentrations were 4.72 ± 0.23 (g/dL), 4.82± 0.27 (g/dL), 4.36 ± 0.15 (g/dL), respectively in mild, moderate and severe in fatty liver and 4.63 ± 0.26 (g/dL) in control group (p= 0.56). Conclusions: Blood pressure and AST activity were significantly higher, but blood sugar was significantly lower in fatty liver. Severity of steatosis and albumin concenteration is statistically significant. Keywords: Fatty liver, Steatosis, Albumin doi:10.1016/j.clinbiochem.2011.08.097
E Poster – [A-10-401-2] Polyethersulfone membranes for cell culture systems Azadbakht Mehria, Madaeni Sayed Siavashb, Sahebjamee Foroodb a Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran b Membrane Research Center, Department of Chemical Engineering, Razi University, Kermanshah, Iran E-mail addresses: [email protected] (A. Mehri), [email protected] (M.S. Siavash), [email protected] (S. Forood)
E Poster – [A-10-411-1] Lowering BNP cut-points is beneficial in obese patients diagnostic Opris Simona, Constantin Gianina Caldarusani 9, Bucharest, Romania E-mail addresses: [email protected] (O. Simona), [email protected] (C. Gianina) Introduction: Physiologically, brain natriuretic peptides (BNP) and lipolysis are closely linked. Obesity has been identified as a major risk factor for the development of cardiovascular diseases (CVD) and has been reported to have an impact on BNP in apparently healthy subjects but also in CVD patients. Thus, we speculate that BNP could play an important role in lipid metabolism and may affect the pathophysiology of obesity in CVD patients. Materials and methods: Serum samples were obtained from CVD elderly patients distributed in 2 groups: I-non-obese and II-obese. The plasma mature form of brain natriuretic peptide (NT-proBNP) was measured by a sandwich enzyme immunoassay with spectrophotometric detection at 450 nm. Results: Our study revealed for group I with BMI (22.77 ± 2.15) higher BNP levels (18.79 ± 16.87 pmol/L) versus group II (16.57 ± 20.81 pmol/L) with BMI 33.33 ± 4.75. Thereby, despite a similar severity of CVD, levels of BNP were 11.81% lower in obese than in non-obese patients. Conclusion: Overall, these data demonstrates that obesity is an important and independent determinant of BNP expression in patients with CVD. Inverse relationship between BNP and body mass index may suggests “beneficial” effects of obesity, but clearly lower levels did not confer a more favourable prognosis. The precise mechanisms linking obesity to CVD remain unsolved and may be due either to release attenuation or increases in clearance receptors. These effects should be taken into account for appropriate BNP reference values, so lower cut-points should be used for obese patients and a higher cut-point for lean patients to increase specificity. Keywords: Natriuretic peptides, Obesity, Cardiovascular diseases
Introduction: Polyethersulfone (PES) membranes with high chemical, thermal and mechanical stability can be used for low-cost membrane preparation. In this study, PES membranes and cell culture inserts were prepared and optimized for utilization in cell culture systems. Materials and methods: Polyethersulfone membranes were prepared using phase inversion via immersion precipitation. The film was moved to the non-solvent bath, a mixture of water and ethanol for precipitation. The membrane was stored in distilled water for 24 h. The performances of non-transparent, transparent and commercial PES membranes were compared. The structures of the membranes were observed by inverted optical microscope. The cyto-compatibility of PES membrane was evaluated by culturing HepG2, a human liver (carcinoma) cell line. Cell viability and adhesion were evaluated. The biological activity of cells was determined by uptake of Indocyanine Green (ICG), and Periodic Acid Schiff (PAS) staining. Results: Although the morphology of non-transparent and transparent PES membranes was different from commercial membrane, they demonstrated similar performances. The cells showed round or oval shape and adhered to form three-dimensional aggregates on the membrane. Cellular uptake of ICG was detected in cells and glycogen storage was determined by PAS staining methods. Conclusion: The activity of the cells cultured on membrane confirmed the acceptable properties of the prepared membranes. The homemade cell culture inserts showed satisfactory performance compared to commercial ones. Keywords: Membrane, Polyethersulfone, Cell culture, HepG2 cell line doi:10.1016/j.clinbiochem.2011.08.098
doi:10.1016/j.clinbiochem.2011.08.099
E Poster – [A-10-427-1] Glucose-6-phosphate dehydrogenase deficiency in the newborn in relation to neonatal hyperbilirubinaemia Mehrotra Vinita, Sharma Anitab, M. Tiwaric a Biochemistry, HIHT, Jollygrant, Doiwala, Dehradun, India b Medicine, HIH, Jollygrant, Doiwala, Dehradun, India c Medicine, HIHT, Jollygrant, Doiwala, Dehradun, India E-mail addresses: [email protected] (M. Vinit), [email protected] (S. Anita), [email protected] (M. Tiwari) Introduction: Hyperbilirubinaemia or jaundice is a common problem in newborn infants. Glucose-6-phosphate dehydrogenase (G6PD) deficiency can cause acute hemolysis during oxidative stress and also severe hyperbilirubinaemia in the newborn. Our aim was to study the prevalence of G6PD deficiency in relation to neonatal jaundice. Materials and methods: We performed G6PD assay in the samples of 280 subjects. Observation of jaundice and determination of bilirubin level as well as work up for other causes of jaundice were made in the G6PD deficiency group compared to a G6PD normal group. 160 were clinically jaundiced and only 106 were included in the study whereas rests were excluded due to presence of direct hyperbilirubinaemia. Results: Neonatal jaundice was found to be affecting 57.14% with male to female ratio of 2.4:1 and common cause was physiological jaundice 42.45%. It was more severe in cases due to Rh incompatibility