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Development and evaluation of a bivalent Shigella flexneri-sonnei I vaccine strain
Conference Abstracts
Development and evaluation of a bivalent Shigellaflexneri-sonnei I vaccine strain V.M. Bondarenko, Yu.E. Polotsky, I.A. Nastichkin, V.E. Efremov, G.V. Smirnova, Yu.M. Romanova and V.V. Utkin
N.F. Gamaleya Institute for Epidemiology and Microbiology, Gamaleya str. 18, Moscow-D98, USSR The bivalent S. flexneri y433-S, sonnei NR18 (Rifr Km r) strain harbouring a plasmid of S. sonnei I phase antigen with inactivation of invasion genes by Tn5 transposon mutagenesis has been obtained. This vaccine strain is negative on Sereny test. Bacteria manifested decreased adhesion and invasion on HEp-2 cells, lost cytotoxicity and ability to intercellular spread. In ligated rabbit gut loops challenged the microbes were also
characterized diminished adhesion to the enterocytes, they were almost devoid of invasive feature. Immunomorphologic response to peroral immunization was associated with bacterial translocation by M-cells of Peyer's patches. After oral immunization of rabbit challenge of their ligated intestinal loops with virulent strains revealed full protection.
Some characteristics of cholera vaccine for oral use L.S. Nazarova, I.V. Isupov, M.N. Dzhaparidze and L.P. Pavlova
Mikrob All Union Plague Res. Inst., Universitetskaya 46, Saratov 410071, USSR Using morphological histochemical immunological and serological tests on adult rabbits, suckling rabbits, some of which were gnotobiots and 3 strains of mice the immunologic efficiency and harmless of cholera chemical vaccine for oral use constructed in Microb All-Union Antiplague Res. Inst. had been studied. Toxicity of this preparation was manifested only
if the dose counted for the mass of the body of animal was equal 700 doses for man. Histopathologic changes were caused mainly by O-antigen. Inflammation in the intestine quickly reduced and immunomorphological changes took place. Immune response of biomodels on the cholera vaccine was genetically determined.
Perspectives to the use of cholera purified enzymes for modelling an artificial-cell vaccine A.K. Adamov, Yu.Yu. Yeliseev, N.V. Mayorov, T.V. Bugorkova
.4ll Union Research Antiplague Institute, "Microbe ', Universitetskaya, 46, 723 Saratov 103, 410071, Saratov, USSR Immunogenic properties of certain Vibrio cholerae purified enzymes was assessed with a view of modelling an artificial-cell cholera vaccine consisting of artificial cells with a microcapsule and cholera enzymes and antigens. Cholera enzymes were isolated from inactivated broth cultures by gel column chromatography: amilase and ATP-ase were obtained from V. cholerae strain 569B, and E. coli HB 101 pRD39 was used as a cholera neuraminidase producing strain. The enzymes demonstrated weak antigenic activity with poor stimulation of
antienzymatic antibodies in rabbits, the titres being as low as 1:128 to neuraminidase, 1:16 to amylase and 1:340 to ATP-ase. At the same time, agglutinins and vibriocidins in the titres from 1:640 to 1:1280 and 1:105 to 1:106, respectively, were detected in the immune sera. Antivibrio inhibitory titres correlated with the presence of antienzymatic antibodies. Antitoxic antibodies could not be detected either. Thus, cholera enzymes, though poor antigenic factors, do induce anticholera immunity in animals.
New oral cholera chemical bivalent vaccine and the results of the controlled epidemiological trial in volunteers M.N. Dzhaparidze, A.V. Naumov, A.A. Sumarokov, A.K. Adamov and Yu.Yu. Yeliseev
All Union Research Antiplague Institute 'Microbe ', Universitetskaya 46, 723 Saratov 103, 410071, USSR Oral cholera vaccine contains 45% O-antigen (serovars Ogawa and Inaba in equal parts) and at least 10 serologically active proteins; of these toxoid (about 60% of total amount of protein) and 8 enzymes. About 300 volunteers aged 19 years and over were placed under observation in the course of the trial of oral cholera vaccine in tablets, for safety, reactogenic properties and immunological effectiveness. Our studies showed
that the vaccine preparations assayed, stimulated the production of specific antitoxins and vibriocidal antibodies to both serovars of cholera vibrios. The oral vaccine was found to have an advantage over parenteral vaccines due to the absence of reactogenic properties and the formation of local immunity: coproantibodies appeared in 80% and 9% of the vaccinees, respectively.
Vaccine, Vol. 10, Issue 4, 1992 285
Development and evaluation of a bivalent Shigellaflexneri-sonnei I vaccine strain V.M. Bondarenko, Yu.E. Polotsky, I.A. Nastichkin, V.E. Efremov, G.V. Smirnova, Yu.M. Romanova and V.V. Utkin
N.F. Gamaleya Institute for Epidemiology and Microbiology, Gamaleya str. 18, Moscow-D98, USSR The bivalent S. flexneri y433-S, sonnei NR18 (Rifr Km r) strain harbouring a plasmid of S. sonnei I phase antigen with inactivation of invasion genes by Tn5 transposon mutagenesis has been obtained. This vaccine strain is negative on Sereny test. Bacteria manifested decreased adhesion and invasion on HEp-2 cells, lost cytotoxicity and ability to intercellular spread. In ligated rabbit gut loops challenged the microbes were also
characterized diminished adhesion to the enterocytes, they were almost devoid of invasive feature. Immunomorphologic response to peroral immunization was associated with bacterial translocation by M-cells of Peyer's patches. After oral immunization of rabbit challenge of their ligated intestinal loops with virulent strains revealed full protection.
Some characteristics of cholera vaccine for oral use L.S. Nazarova, I.V. Isupov, M.N. Dzhaparidze and L.P. Pavlova
Mikrob All Union Plague Res. Inst., Universitetskaya 46, Saratov 410071, USSR Using morphological histochemical immunological and serological tests on adult rabbits, suckling rabbits, some of which were gnotobiots and 3 strains of mice the immunologic efficiency and harmless of cholera chemical vaccine for oral use constructed in Microb All-Union Antiplague Res. Inst. had been studied. Toxicity of this preparation was manifested only
if the dose counted for the mass of the body of animal was equal 700 doses for man. Histopathologic changes were caused mainly by O-antigen. Inflammation in the intestine quickly reduced and immunomorphological changes took place. Immune response of biomodels on the cholera vaccine was genetically determined.
Perspectives to the use of cholera purified enzymes for modelling an artificial-cell vaccine A.K. Adamov, Yu.Yu. Yeliseev, N.V. Mayorov, T.V. Bugorkova
.4ll Union Research Antiplague Institute, "Microbe ', Universitetskaya, 46, 723 Saratov 103, 410071, Saratov, USSR Immunogenic properties of certain Vibrio cholerae purified enzymes was assessed with a view of modelling an artificial-cell cholera vaccine consisting of artificial cells with a microcapsule and cholera enzymes and antigens. Cholera enzymes were isolated from inactivated broth cultures by gel column chromatography: amilase and ATP-ase were obtained from V. cholerae strain 569B, and E. coli HB 101 pRD39 was used as a cholera neuraminidase producing strain. The enzymes demonstrated weak antigenic activity with poor stimulation of
antienzymatic antibodies in rabbits, the titres being as low as 1:128 to neuraminidase, 1:16 to amylase and 1:340 to ATP-ase. At the same time, agglutinins and vibriocidins in the titres from 1:640 to 1:1280 and 1:105 to 1:106, respectively, were detected in the immune sera. Antivibrio inhibitory titres correlated with the presence of antienzymatic antibodies. Antitoxic antibodies could not be detected either. Thus, cholera enzymes, though poor antigenic factors, do induce anticholera immunity in animals.
New oral cholera chemical bivalent vaccine and the results of the controlled epidemiological trial in volunteers M.N. Dzhaparidze, A.V. Naumov, A.A. Sumarokov, A.K. Adamov and Yu.Yu. Yeliseev
All Union Research Antiplague Institute 'Microbe ', Universitetskaya 46, 723 Saratov 103, 410071, USSR Oral cholera vaccine contains 45% O-antigen (serovars Ogawa and Inaba in equal parts) and at least 10 serologically active proteins; of these toxoid (about 60% of total amount of protein) and 8 enzymes. About 300 volunteers aged 19 years and over were placed under observation in the course of the trial of oral cholera vaccine in tablets, for safety, reactogenic properties and immunological effectiveness. Our studies showed
that the vaccine preparations assayed, stimulated the production of specific antitoxins and vibriocidal antibodies to both serovars of cholera vibrios. The oral vaccine was found to have an advantage over parenteral vaccines due to the absence of reactogenic properties and the formation of local immunity: coproantibodies appeared in 80% and 9% of the vaccinees, respectively.
Vaccine, Vol. 10, Issue 4, 1992 285