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Development and validation of a model that estimates body fat percentage based on simple anthropometric measurements
Abstracts / Clinical Nutrition ESPEN 13 (2016) e55ee74
Materials and methods: 107 patients diagnosed with cancer and treated in an oncology centre of excellence who have been referred to the community nursing home-care service. A pilot cross-sectional quantitative study, based on convenience sampling. The screening tool used to determine malnutrition was the ESPEN Nutrition Day questionnaire for oncological patients in greek language, as translated and validated by GrESPEN. Results: On average, a weight loss of 8 kilograms over the last year was noted for male patients, contrary to women, for whom no change was noted. In 76% of cases the weight loss was unintentional and 13% of the sample population has already lost more than 20% body weight. Minimum BMI was 19.8 with a good correlation to cancer stage. Risk factors for malnutrition identified included taste distortion, anorexia and constipation. None of the individuals at risk had been referred for nutrition counseling/support before the study. Conclusions: The community nurses should promote cancer patients’ education on nutritional status screening within the context of multidisciplinary nutrition teams. STUDY OF POSSIBLE BENEFICIAL EFFECT OF CROCIN IN AN ANIMAL MODEL OF DIABETES 1
2
Nantia Apostolidou , Eleftheria Konstantinos Feidantsis , Galatou 1, Antigone Lazou 1. 1 Laboratory of Animal Physiology, Department of Zoology, School of Biology, Aristotle University of Thessaloniki, GR 54124, Greece; 2 Alexander Technological Educational Institution of Thessaloniki, Department of Nutrition and Dietetics, Thessaloniki, Greece Introduction: Diabetes mellitus is a metabolic disease whose worldwide incidence is high (4-5%). The connection between diabetes with poor metabolic control and the high mortality due to cardiovascular disease, hyperlipidemia, nephropathies and neuropathies are very well established. Several recent studies demonstrate the protective effect of a wide variety of antioxidants to tissue damage as in the case of myocardial infarction. The extract of the plant Crocus sativus L. (commonly known as saffron) contains compounds, such as crocin, picrocrocin and safranal. Among the pharmacologically active ingredients, crocin is the most important and abundant antioxidant, has antitumor, antioxidant, anti-hyperlipidemic properties and is known to strengthen memory. Aim: The purpose of this study is the possible improvement of heart, liver and kidney function of diabetic rats after oral administration of crocin (10 and 20 mg / kg body weight) for 2 weeks and identification of the underlying molecular mechanisms that contribute to the improvement of cardiac, hepatic and renal function of the animals. Materials and methods: Specifically levels of biochemical indicators such as glucose, triglycerides, urea, total cholesterol, HDL and LDL and levels of enzymes SGOT, SGPT and GGT were detected in animal blood plasma. Results:
e65
Figure 2. SGOT, SGPT and gGT levels in control, diabetic and diabetic rats after the oral administration of crocin (10 and 20 mg/kg body weight). Values are means ± SD; n¼5 preparations from different animals. *P<0.05 compared to control animals, +P<0.05 compared to diabetic animals.
Conclusions: These indexes are restored to levels close to those of control compared to diabetic animals, confirming the ameliorating effect of crocin. For a more thorough investigation of the role of crocin in diabetes, it is necessary to study other molecular and biochemical markers. DIETARY HABITS OF WORKERS IN 301 ARMY DEPOT AND CORRELATION WITH THEIR BODY TYPE AND BEHAVIORAL INDICATORS Konstantina-Rafaella Mavropoulou 1, Stamatia Krachala 1, Nikolaos Rigopoulos 1, Charalampos Titakis 2, Misail Papadakis 2, Antonios Koutelidakis 1, a. 1 University of Aegean, Department of Food Science and Nutrition, Mitropoliti Ioakeim 2, Myrina, Lemnos, Greece; 2 301 ARMY DEPOT, Agioi Anargyroi, Attica, Greece Introduction: The diet of workers in a military Depot may affect their mental and physical performance. Aim: To investigate the dietary habits of 301 ARMY DEPOT employees and its correlation to the Body Mass Index (BMI) and behavioral factors, with aim to inform and encourage them for a proper diet that will lead to the optimization of work efficiency and the promotion of their health. Materials and methods: 166 men and women workers of 301 ARMY DEPOT completed, on a voluntary base and under the guidance of researchers, a food frequency questionnaire (FFQ). After BMI calculation, based on the information completed by the volunteers, the percentages of obese, overweight and underweight individuals were calculated. The results processing was performed with the Statistical Programme SPSS v1.8 by using correlation coefficients and applying T-test and Anova. Results: The diet of workers of 301 ARMY DEPOT seems to be close to the dietary recommendations, with women adopt healthier diet than men. 70% of men and 40% of women have frequent physical activity. 70% of workers said that follow little or no healthy diet, while 30% was smokers. 54% of men and 80% of women rarely dine in the unit restaurant. 52% of workers are of normal weight, 30% are overweight, 15% obese and 1.2% underweight. The BMI of workers was associated with skipping meals (30% skip breakfast), and with the number of daily meals (50% consume 2 or 3 meals daily) (p<0,05). Smoking and physical activity inside and outside of the work was not correlated to the foods frequency consumption (p>0,05). Conclusion: The implementation of a nutrition education program with aim to improve dietary habits, may lead to improved work efficiency and promoting the health of workers. DEVELOPMENT AND VALIDATION OF A MODEL THAT ESTIMATES BODY FAT PERCENTAGE BASED ON SIMPLE ANTHROPOMETRIC MEASUREMENTS Spyridon Kanellakis 1, Efstathios Vladlena Khudokonenko 1, Eftychia Apostolidou 1, Loukia Gerakiti 1, Maria Skoufas 1, Andrioti 1, Christianna Georgopoulou 3, Georgia Ziogou 2, Theodora Makridi 2, Yannis Manios 1. 1 Department of Nutrition and Dietetics, Harokopio University, Athens, Greece; 2 Department of Nutrition and Dietetics, T.E.I. of Thessaly, Karditsa, Greece; 3 Department of Nutrition and Dietetics, T.E.I. of Crete, Sitia, Greece
Figure 1. Glucose, triglycerides, urea, total cholesterol, HDL and LDL levels in control, diabetic and diabetic rats after the oral administration of crocin (10 and 20 mg/kg body weight). Values are means ± SD; n¼5 preparations from different animals. *P<0.05 compared to control animals, +P<0.05 compared to diabetic animals.
a The study was carried out as part of a nutritional educational program that is performed from Aegean University (Responsible Prof.: Lecturer PD 407 Koutelidakis A.) in collaboration with 301 ARMY DEPOT (Responsible from 301 Depot: C. Z. Titakis, PhD candidate, Dept of Chemical Engineering, National Technical University of Athens)
e66
Abstracts / Clinical Nutrition ESPEN 13 (2016) e55ee74
Aim: The aim of the current study was to develop a simple, valid and low cost model that estimates total body fat percentage (%TBF) based on measures obtained only with a measuring tape. Materials and methods: The study population was 642 healthy adults (408 women and 234 men) with mean age 41.3 years (18 to 75 years) and mean body mass index (BMI) 25.86 kg/m2. A full set of anthropometric data including weight, height, perimeters and skinfolds were obtained. The volunteers were randomized into a developing (n ¼ 426) and a validation group (n ¼ 214). Dual energy x-ray absorptiometry was used as a reference method. The model was formed with linear regression and its validity and reliability were determined by Bland Altman and Intraclass Correlation Coefficient tests. Results: The developed model is %BF¼ - 0,615 e 10,498 x Gender + 0,321 x waist circumference(cm) + 0,502 x hip circumference(cm) e 0,390 x forearm circumference(cm) e 19,678 x height(m) (r¼0,923, p< 0.001). The model had no significant bias (-0,052%), limits of agreement ± 8,1% and high Іntra Class Correlation Coefficient (0,955). ASSESSMENT OF ANTHROPOMETRY, DIETARY HABITS AND PHYSICAL ACTIVITY LEVEL OF GREEK STUDENTS: RESULTS FROM THE «ЕYΖНN» PROGRAM (NATIONAL ACTION FOR CHILDREN’S HEALTH) 2013-2014 Michael Georgoulis, Glykeria Psarra, Konstantinos Tambalis, Demosthenes Panagiotakos, Labros Sidossis. Department of Nutrition and Dietetics, School of Health Science and Education, Harokopio University, Athens, Greece Introduction: The evaluation of children’s anthropometric characteristics, dietary habits and physical activity level is important in terms of public health, however such an effort has not yet been made on a large scale in Greece. Aim: The anthropometric and lifestyle assessment of a representative sample of Greek students. Materials and methods: 473.665 students from 4.792 Primary and Secondary Education schools of all geographical regions of Greece, who participated in the «ЕYΖНN» program during the school year 2013-14. The evaluation of student’s anthropometric indices was conducted from physical education teachers and their lifestyle habits were assessed through a questionnaire. The program was financially supported by OPAP SA. Results: 22.3% of the students were classified as overweight (boys: 22.8%, girls: 21.7%) and 8.6% as obese (boys: 9.4%, girls: 7.8%). About half of the students (45.1%) were characterized by insufficient levels of physical activity, girls in a greater extent than boys (52.9% versus 37.5%), however a higher percentage of boys seemed to exceed 2 hours of screen time per day compared to girls (28.1% versus 21.5%). The level of adherence to the Mediterranean diet was found low in 63.6% of the participating students, to a similar extent in boys and girls (boys: 63.9%, girls: 62.3%), and appeared to be progressively decreasing from Elementary to High School (boys: -18.3%, girls: -23.6%). Conclusions: A significant proportion of Greek schoolchildren are characterized by excess body weight, high sedentariness and poor dietary habits. Interventions aiming at promoting a healthy lifestyle in the country’s student population are urgently needed. LIFESTYLE DETERMINANTS OF CHILDHOOD OBESITY: RESULTS FROM THE «ЕYΖНN» PROGRAM (NATIONAL ACTION FOR CHILDREN’S HEALTH) 2013-2014 Maria Maraki, Michael Georgoulis, Glykeria Psarra, Konstantinos Tambalis, Demosthenes Panagiotakos, Labros Sidossis. Department of Nutrition and Dietetics, School of Health Science and Education, Harokopio University, Athens, Greece Introduction: Dietary, physical activity and sleep habits are associated with body weight; however, their combined effect on the likelihood of childhood obesity has not been investigated in large scale epidemiological studies in Greece. Aim: To investigate the association between lifestyle and the weight status of a representative sample of Greek students. Materials and methods: 258.563 students aged 8-19 years from all geographical regions of Greece, who participated in the «ЕYΖНN» program
during the school year 2013-14. The evaluation of student’s anthropometric indices was conducted from physical education teachers and their lifestyle (dietary, physical activity and sleep habits) was assessed through a questionnaire. The program was financially supported by OPAP SA. Results: According to multiple logistic regression analysis, low adherence to the Mediterranean diet (KIDMED score 3), low sleep duration (<9 and <8 hours per day for children 8-10 and 10-19 years old, respectively) and increased time spent in sedentary activities (2 hours of screen time per day) were associated with 17% (95%CI¼1.11-1.23), 23% (95%CI¼1.19-1.17) and 21% (95%CI¼1.17-1.25) increased likelihood of obesity, respectively, after adjusting for children’s gender and age. By contrast, for each 1-day per week increase in the frequency of organized physical activity, the likelihood of childhood obesity was decreased by 6% (95%CI¼0.93-0.94), after adjusting for the aforementioned factors. Conclusions: Given their independent association with children’s weight status, dietary, physical activity and sleep habits should be assessed and taken into account in the context of health promotion programs and lifestyle interventions aiming at the prevention and treatment of childhood obesity. PAST TRENDS OF OBESITY ATTRIBUTABLE MORTALITY IN THE NETHERLANDS; AN APPLICATION OF AGE-PERIOD e COHORT ANALYSIS Nikoletta Vidra 1, Maarten J. Bijlsma 2, Fanny Janssen 1, 3. 1 Population Research Centre, Faculty of Spatial Sciences, University of Groningen, The Netherlands; 2 Unit PharmacoEpidemiology & PharmacoEconomics (PE2), Department of Pharmacy, University of Groningen, The Netherlands; 3 Netherlands Interdisciplinary Demographic Institute, The Hague, The Netherlands Introduction: Obesity has dramatically increased over time and constitutes a major health burden which can be estimated by the share of total deaths due to obesity (obesity-attributable mortality). Previous estimates varied widely according to the methodology used, as previous studies didn’t always account for the multiple dimensions of the obesity epidemic: age, period and birth cohort. Aim: To better capture the complexity of the obesity epidemic and its impact on mortality by assessing age, period and birth cohort effects and patterns in the Netherlands, in the period 1981 to 2010. Materials and methods: Data (by age and sex) were obtained from the following sources: Obesity prevalence from Statistics Netherlands, Relative Risks (RR) of dying from obesity from a recent meta-analysis and all-cause mortality by Human Mortality Database. We applied age-period-cohort analysis to assess obesity-attributable mortality by sex in the Netherlands. Results: Obesity-attributable mortality doubled in the Netherlands between 1981 and 2010; in men, the fraction of mortality due to obesity rose from 0.7 % to 1.3 % while in women from 1.0 to 2.0 %. The effect of birth cohort to obesity-attributable mortality was larger among women as compared to men, and for women even more important that the effect of period. In specific, for women born after 1941-1945, obesity-attributable mortality is increasing with every next generation. Conclusions: Next to age and period a substantial effect of birth cohort on obesity-attributable mortality was shown, especially in women. Future studies on obesityeattributable mortality should not ignore the multiple dimensions of obesity. AFLATOXIN M1 LEVELS IN MILK AND DAIRY PRODUCTS IN GREECE e RELATION TO PUBLIC HEALTH Eleni Malissiova 1, Eymorfia Maraidoni 1, Dimitra Kyriazi 1, Michael Gonidakis 2, Athanasios Manouras 1, Olga Gortzi 1, Constantinos Deligiannis 2. 1 Technological Educational Institute of Thessaly, Food Technology Department, Karditsa, Greece; 2 Technological Educational Institute of Thessaly, Nutrition & Dietetics Department, Karditsa, Greece Introduction: Aflatoxin M1 (AFM1) contaminates milk and dairy products that are consumed very often by children, thus ultimately exposed to the toxic action and have negative health effects. Aim: The aim of this study was to assess the contamination levels with AFM1, of different milk types and dairy in the Greek market and also to assess the relation to Public Health.
Materials and methods: 107 patients diagnosed with cancer and treated in an oncology centre of excellence who have been referred to the community nursing home-care service. A pilot cross-sectional quantitative study, based on convenience sampling. The screening tool used to determine malnutrition was the ESPEN Nutrition Day questionnaire for oncological patients in greek language, as translated and validated by GrESPEN. Results: On average, a weight loss of 8 kilograms over the last year was noted for male patients, contrary to women, for whom no change was noted. In 76% of cases the weight loss was unintentional and 13% of the sample population has already lost more than 20% body weight. Minimum BMI was 19.8 with a good correlation to cancer stage. Risk factors for malnutrition identified included taste distortion, anorexia and constipation. None of the individuals at risk had been referred for nutrition counseling/support before the study. Conclusions: The community nurses should promote cancer patients’ education on nutritional status screening within the context of multidisciplinary nutrition teams. STUDY OF POSSIBLE BENEFICIAL EFFECT OF CROCIN IN AN ANIMAL MODEL OF DIABETES 1
2
Nantia Apostolidou , Eleftheria Konstantinos Feidantsis , Galatou 1, Antigone Lazou 1. 1 Laboratory of Animal Physiology, Department of Zoology, School of Biology, Aristotle University of Thessaloniki, GR 54124, Greece; 2 Alexander Technological Educational Institution of Thessaloniki, Department of Nutrition and Dietetics, Thessaloniki, Greece Introduction: Diabetes mellitus is a metabolic disease whose worldwide incidence is high (4-5%). The connection between diabetes with poor metabolic control and the high mortality due to cardiovascular disease, hyperlipidemia, nephropathies and neuropathies are very well established. Several recent studies demonstrate the protective effect of a wide variety of antioxidants to tissue damage as in the case of myocardial infarction. The extract of the plant Crocus sativus L. (commonly known as saffron) contains compounds, such as crocin, picrocrocin and safranal. Among the pharmacologically active ingredients, crocin is the most important and abundant antioxidant, has antitumor, antioxidant, anti-hyperlipidemic properties and is known to strengthen memory. Aim: The purpose of this study is the possible improvement of heart, liver and kidney function of diabetic rats after oral administration of crocin (10 and 20 mg / kg body weight) for 2 weeks and identification of the underlying molecular mechanisms that contribute to the improvement of cardiac, hepatic and renal function of the animals. Materials and methods: Specifically levels of biochemical indicators such as glucose, triglycerides, urea, total cholesterol, HDL and LDL and levels of enzymes SGOT, SGPT and GGT were detected in animal blood plasma. Results:
e65
Figure 2. SGOT, SGPT and gGT levels in control, diabetic and diabetic rats after the oral administration of crocin (10 and 20 mg/kg body weight). Values are means ± SD; n¼5 preparations from different animals. *P<0.05 compared to control animals, +P<0.05 compared to diabetic animals.
Conclusions: These indexes are restored to levels close to those of control compared to diabetic animals, confirming the ameliorating effect of crocin. For a more thorough investigation of the role of crocin in diabetes, it is necessary to study other molecular and biochemical markers. DIETARY HABITS OF WORKERS IN 301 ARMY DEPOT AND CORRELATION WITH THEIR BODY TYPE AND BEHAVIORAL INDICATORS Konstantina-Rafaella Mavropoulou 1, Stamatia Krachala 1, Nikolaos Rigopoulos 1, Charalampos Titakis 2, Misail Papadakis 2, Antonios Koutelidakis 1, a. 1 University of Aegean, Department of Food Science and Nutrition, Mitropoliti Ioakeim 2, Myrina, Lemnos, Greece; 2 301 ARMY DEPOT, Agioi Anargyroi, Attica, Greece Introduction: The diet of workers in a military Depot may affect their mental and physical performance. Aim: To investigate the dietary habits of 301 ARMY DEPOT employees and its correlation to the Body Mass Index (BMI) and behavioral factors, with aim to inform and encourage them for a proper diet that will lead to the optimization of work efficiency and the promotion of their health. Materials and methods: 166 men and women workers of 301 ARMY DEPOT completed, on a voluntary base and under the guidance of researchers, a food frequency questionnaire (FFQ). After BMI calculation, based on the information completed by the volunteers, the percentages of obese, overweight and underweight individuals were calculated. The results processing was performed with the Statistical Programme SPSS v1.8 by using correlation coefficients and applying T-test and Anova. Results: The diet of workers of 301 ARMY DEPOT seems to be close to the dietary recommendations, with women adopt healthier diet than men. 70% of men and 40% of women have frequent physical activity. 70% of workers said that follow little or no healthy diet, while 30% was smokers. 54% of men and 80% of women rarely dine in the unit restaurant. 52% of workers are of normal weight, 30% are overweight, 15% obese and 1.2% underweight. The BMI of workers was associated with skipping meals (30% skip breakfast), and with the number of daily meals (50% consume 2 or 3 meals daily) (p<0,05). Smoking and physical activity inside and outside of the work was not correlated to the foods frequency consumption (p>0,05). Conclusion: The implementation of a nutrition education program with aim to improve dietary habits, may lead to improved work efficiency and promoting the health of workers. DEVELOPMENT AND VALIDATION OF A MODEL THAT ESTIMATES BODY FAT PERCENTAGE BASED ON SIMPLE ANTHROPOMETRIC MEASUREMENTS Spyridon Kanellakis 1, Efstathios Vladlena Khudokonenko 1, Eftychia Apostolidou 1, Loukia Gerakiti 1, Maria Skoufas 1, Andrioti 1, Christianna Georgopoulou 3, Georgia Ziogou 2, Theodora Makridi 2, Yannis Manios 1. 1 Department of Nutrition and Dietetics, Harokopio University, Athens, Greece; 2 Department of Nutrition and Dietetics, T.E.I. of Thessaly, Karditsa, Greece; 3 Department of Nutrition and Dietetics, T.E.I. of Crete, Sitia, Greece
Figure 1. Glucose, triglycerides, urea, total cholesterol, HDL and LDL levels in control, diabetic and diabetic rats after the oral administration of crocin (10 and 20 mg/kg body weight). Values are means ± SD; n¼5 preparations from different animals. *P<0.05 compared to control animals, +P<0.05 compared to diabetic animals.
a The study was carried out as part of a nutritional educational program that is performed from Aegean University (Responsible Prof.: Lecturer PD 407 Koutelidakis A.) in collaboration with 301 ARMY DEPOT (Responsible from 301 Depot: C. Z. Titakis, PhD candidate, Dept of Chemical Engineering, National Technical University of Athens)
e66
Abstracts / Clinical Nutrition ESPEN 13 (2016) e55ee74
Aim: The aim of the current study was to develop a simple, valid and low cost model that estimates total body fat percentage (%TBF) based on measures obtained only with a measuring tape. Materials and methods: The study population was 642 healthy adults (408 women and 234 men) with mean age 41.3 years (18 to 75 years) and mean body mass index (BMI) 25.86 kg/m2. A full set of anthropometric data including weight, height, perimeters and skinfolds were obtained. The volunteers were randomized into a developing (n ¼ 426) and a validation group (n ¼ 214). Dual energy x-ray absorptiometry was used as a reference method. The model was formed with linear regression and its validity and reliability were determined by Bland Altman and Intraclass Correlation Coefficient tests. Results: The developed model is %BF¼ - 0,615 e 10,498 x Gender + 0,321 x waist circumference(cm) + 0,502 x hip circumference(cm) e 0,390 x forearm circumference(cm) e 19,678 x height(m) (r¼0,923, p< 0.001). The model had no significant bias (-0,052%), limits of agreement ± 8,1% and high Іntra Class Correlation Coefficient (0,955). ASSESSMENT OF ANTHROPOMETRY, DIETARY HABITS AND PHYSICAL ACTIVITY LEVEL OF GREEK STUDENTS: RESULTS FROM THE «ЕYΖНN» PROGRAM (NATIONAL ACTION FOR CHILDREN’S HEALTH) 2013-2014 Michael Georgoulis, Glykeria Psarra, Konstantinos Tambalis, Demosthenes Panagiotakos, Labros Sidossis. Department of Nutrition and Dietetics, School of Health Science and Education, Harokopio University, Athens, Greece Introduction: The evaluation of children’s anthropometric characteristics, dietary habits and physical activity level is important in terms of public health, however such an effort has not yet been made on a large scale in Greece. Aim: The anthropometric and lifestyle assessment of a representative sample of Greek students. Materials and methods: 473.665 students from 4.792 Primary and Secondary Education schools of all geographical regions of Greece, who participated in the «ЕYΖНN» program during the school year 2013-14. The evaluation of student’s anthropometric indices was conducted from physical education teachers and their lifestyle habits were assessed through a questionnaire. The program was financially supported by OPAP SA. Results: 22.3% of the students were classified as overweight (boys: 22.8%, girls: 21.7%) and 8.6% as obese (boys: 9.4%, girls: 7.8%). About half of the students (45.1%) were characterized by insufficient levels of physical activity, girls in a greater extent than boys (52.9% versus 37.5%), however a higher percentage of boys seemed to exceed 2 hours of screen time per day compared to girls (28.1% versus 21.5%). The level of adherence to the Mediterranean diet was found low in 63.6% of the participating students, to a similar extent in boys and girls (boys: 63.9%, girls: 62.3%), and appeared to be progressively decreasing from Elementary to High School (boys: -18.3%, girls: -23.6%). Conclusions: A significant proportion of Greek schoolchildren are characterized by excess body weight, high sedentariness and poor dietary habits. Interventions aiming at promoting a healthy lifestyle in the country’s student population are urgently needed. LIFESTYLE DETERMINANTS OF CHILDHOOD OBESITY: RESULTS FROM THE «ЕYΖНN» PROGRAM (NATIONAL ACTION FOR CHILDREN’S HEALTH) 2013-2014 Maria Maraki, Michael Georgoulis, Glykeria Psarra, Konstantinos Tambalis, Demosthenes Panagiotakos, Labros Sidossis. Department of Nutrition and Dietetics, School of Health Science and Education, Harokopio University, Athens, Greece Introduction: Dietary, physical activity and sleep habits are associated with body weight; however, their combined effect on the likelihood of childhood obesity has not been investigated in large scale epidemiological studies in Greece. Aim: To investigate the association between lifestyle and the weight status of a representative sample of Greek students. Materials and methods: 258.563 students aged 8-19 years from all geographical regions of Greece, who participated in the «ЕYΖНN» program
during the school year 2013-14. The evaluation of student’s anthropometric indices was conducted from physical education teachers and their lifestyle (dietary, physical activity and sleep habits) was assessed through a questionnaire. The program was financially supported by OPAP SA. Results: According to multiple logistic regression analysis, low adherence to the Mediterranean diet (KIDMED score 3), low sleep duration (<9 and <8 hours per day for children 8-10 and 10-19 years old, respectively) and increased time spent in sedentary activities (2 hours of screen time per day) were associated with 17% (95%CI¼1.11-1.23), 23% (95%CI¼1.19-1.17) and 21% (95%CI¼1.17-1.25) increased likelihood of obesity, respectively, after adjusting for children’s gender and age. By contrast, for each 1-day per week increase in the frequency of organized physical activity, the likelihood of childhood obesity was decreased by 6% (95%CI¼0.93-0.94), after adjusting for the aforementioned factors. Conclusions: Given their independent association with children’s weight status, dietary, physical activity and sleep habits should be assessed and taken into account in the context of health promotion programs and lifestyle interventions aiming at the prevention and treatment of childhood obesity. PAST TRENDS OF OBESITY ATTRIBUTABLE MORTALITY IN THE NETHERLANDS; AN APPLICATION OF AGE-PERIOD e COHORT ANALYSIS Nikoletta Vidra 1, Maarten J. Bijlsma 2, Fanny Janssen 1, 3. 1 Population Research Centre, Faculty of Spatial Sciences, University of Groningen, The Netherlands; 2 Unit PharmacoEpidemiology & PharmacoEconomics (PE2), Department of Pharmacy, University of Groningen, The Netherlands; 3 Netherlands Interdisciplinary Demographic Institute, The Hague, The Netherlands Introduction: Obesity has dramatically increased over time and constitutes a major health burden which can be estimated by the share of total deaths due to obesity (obesity-attributable mortality). Previous estimates varied widely according to the methodology used, as previous studies didn’t always account for the multiple dimensions of the obesity epidemic: age, period and birth cohort. Aim: To better capture the complexity of the obesity epidemic and its impact on mortality by assessing age, period and birth cohort effects and patterns in the Netherlands, in the period 1981 to 2010. Materials and methods: Data (by age and sex) were obtained from the following sources: Obesity prevalence from Statistics Netherlands, Relative Risks (RR) of dying from obesity from a recent meta-analysis and all-cause mortality by Human Mortality Database. We applied age-period-cohort analysis to assess obesity-attributable mortality by sex in the Netherlands. Results: Obesity-attributable mortality doubled in the Netherlands between 1981 and 2010; in men, the fraction of mortality due to obesity rose from 0.7 % to 1.3 % while in women from 1.0 to 2.0 %. The effect of birth cohort to obesity-attributable mortality was larger among women as compared to men, and for women even more important that the effect of period. In specific, for women born after 1941-1945, obesity-attributable mortality is increasing with every next generation. Conclusions: Next to age and period a substantial effect of birth cohort on obesity-attributable mortality was shown, especially in women. Future studies on obesityeattributable mortality should not ignore the multiple dimensions of obesity. AFLATOXIN M1 LEVELS IN MILK AND DAIRY PRODUCTS IN GREECE e RELATION TO PUBLIC HEALTH Eleni Malissiova 1, Eymorfia Maraidoni 1, Dimitra Kyriazi 1, Michael Gonidakis 2, Athanasios Manouras 1, Olga Gortzi 1, Constantinos Deligiannis 2. 1 Technological Educational Institute of Thessaly, Food Technology Department, Karditsa, Greece; 2 Technological Educational Institute of Thessaly, Nutrition & Dietetics Department, Karditsa, Greece Introduction: Aflatoxin M1 (AFM1) contaminates milk and dairy products that are consumed very often by children, thus ultimately exposed to the toxic action and have negative health effects. Aim: The aim of this study was to assess the contamination levels with AFM1, of different milk types and dairy in the Greek market and also to assess the relation to Public Health.