Development of 24 h in vitro matured bovine oocytes following parthenogenetic activation by ethanol and cycloheximide treatment

Theriogenology41:277, 1994 DEVELOPMENT OF 24 h IN VITRO MATUREDBOVINE OOCYTESFOLLOWING PARTHENOGENETIC ACTIVATION BY ETHANOLAND CYCLOHEXIMIDE TREATMENT G. A. Presicce and X. Yang Department of Animal Science, Cornell University Ithaca, New York 14853-4801 USA Parthenogenetic activation of in vitro matured (IVM) young bovine oocytes remains a key inefficient step in the current nuclear transfer procedure. Our previous studies have shown that IVM oocytes (23-24 h) form pronuclei at high rates (90-95%) following a combined ethanol and cycloheximide treatment. In the present study, a series of experiments was undertaken to improve development rate of oocytes matured for 24 h and treated by various combinations of ethanol and cycloheximide. Three to four2replicates were conducted in each experiment. Data were analyzed by X . In Experiment I, oocytes (n=Sgl) were treated with 7% ethanol for 5 min followed by incubation in 10 pg/ml cycloheximide in Medium 199 for 0 (control), 5, 10 and 20 h and then cultured for three days in cycloheximide-free medium to allow cleavage development. They were evaluated morphologically and then stained with 10 pg/ml Hoechst 33342 to confirm cleavage development. The cleavage rates, respectively, for the four treatment groups were: 6%, 41%, 34% and 32% (P
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