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Development of novel nano-carrier with safety for vaccine against infectious diseases
Abstracts / Toxicology Letters 259S (2016) S4–S62
signaling pathways and their effect on the immune system and potential testing strategies, including HR assays will be reviewed. Materials and methods: HR assays are performed in mice or rats in a 28-day repeat dose protocol. Animals are dosed with the test compound for 7 days, infected with the challenge infectious agent and clearance of the infectious agent evaluated during the remainder of the 28 day period. The test agent is evaluated in three separate dose levels, compared with a vehicle control and a positive immunomodulatory control. Results: The effect of a test agent is measured by comparing clearance of the infectious agent as well as functional activity of one or more individual immune functions with the vehicle control and a positive immunomodulatory control. Conclusions: TLRs may exert either therapeutic or adverse immunotoxicity and each TLR must be evaluated separately. Financial support: Burleson Research Technologies, Inc. (BRT). http://dx.doi.org/10.1016/j.toxlet.2016.07.042 S01-4 Development of novel nano-carrier with safety for vaccine against infectious diseases Y. Yoshioka Vaccine Creation Project, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan As the recent Ebola virus outbreak in Africa and worldwide influenza pandemic have powerfully demonstrated, society demands the development of vaccines for infectious diseases. However, most protein antigens, such as non-living macromolecules or protein-subunit antigens, evoke only weak or undetectable adaptive immune responses. Therefore, to develop effective vaccines, it is necessary to develop vaccine adjuvants and antigen delivery carriers. In addition, to develop optimal vaccines for clinical applications, it is important to understand the mechanism of action of vaccines on the immune system in terms of efficacy as well as safety. In this regard, we have examined the relationships between physicochemical properties, bio-distribution, intracellular localization and biological responses (safety) of nano-carrier. In addition, recently, we are trying to develop novel nano-carrier with safety for vaccine against infectious diseases. That is, our research is focused on optimizing vaccines related to drug delivery systems and safety science. In this presentation, I will introduce our recent findings about vaccine nano-carrier with efficacy and safety. http://dx.doi.org/10.1016/j.toxlet.2016.07.043 Symposia S02: Effects of pesticides on human health and ways of prevention and control S02-1 Effects of pesticides on human health and ways of prevention and control: “custody” P. Calle-Uribe NorthLAN Stewardship Manager for Crop Protection, Department of Toxicology, LAN, Bogota, Bogota, Colombia Introduction: In 2050: A third more mouths to feed. We live on a planet with more than 7 billion people all with the same or greater needs for food, clean water, sanitation, housing, mobility, education and adequate health care, that ensures their welfare.
S5
Objective: Achieve a healthy and friendly production environment through the custody of the promoter’s products of food production. Materials and methods: The company as a leader in the development of new technologies for the field company combines this factor, with the implementation of programs that allow not only the transfer of technological knowledge but also the promotion of good agricultural practices, aimed towards the same goal: better results with less inputs, all focused on the search for a sustainable agriculture is an important contribution to food security. Results: Custody of the company’s products is the best example. Strengthens the commitments of the company with the “International Code of Conduct on the Distribution and Use of Pesticides” of the Food and Agriculture Organization – FAO. This program provides information and training to representatives in the field, distributors, customers and communities in general, responsible management products companies, in order to prevent any kind of adversity that may happen in relation to the use of products, inputs, processes, operations and waste materials resulting from their work. Conclusions: The continued commitment of the company as a responsible member of society, manifested in its constant concern for continuous improvement, providing a healthy working environment and safe for all its employees and seeking primarily to minimize the environmental impact of their actions and optimize the responsible use of natural resources. Financial support: The company is in charge of project financing of custody. http://dx.doi.org/10.1016/j.toxlet.2016.07.102 S02-2 Poisonings by pesticides in Guatemala M.C. Guzmán-Quilo Department of Toxicology, Centro de Información y Asesoría Toxicológica, Escuela de Química Farmacéutica, Facultad de Ciencias Químicas y Farmacia, Universidad de San Carlos de Guatemala, Guatemala Introduction: Guatemala is a country located at the northern of Central America. One of its main economical activities is agriculture. Agriculture also represents the main percentage of employment. As the rest of Central American countries, pesticides are the main causal agent of acute poisonings. According to a research developed by PLAGSALUD-PAHO on 2000, Guatemala’s registry system had a 97.5% of under registered cases. Objective: To compare the poisoning cases registered at the national service, including the geographical occurrence, population characterization by age, with the group of pesticides according to the chemical structure and poisoning circumstances. Materials and methods: 2013–2015 registries from the Epidemiology National Center were evaluated. These were compared to the population range of ages, the chemical groups of pesticides involved and the poisoning circumstances. Results: It was determined that the number of cases registered from 2013 to 2015 has decreased. The population involved includes the two extremes of life: children and the elderly, but most of the cases are recorded to be middle age workers. The three main chemical groups of pesticides poisoning identified were: organophosphorus, paraquat and phosphine. The circumstances according to the registries go from accidental cases on working activities up to the intentional cases because of suicide attempts.
signaling pathways and their effect on the immune system and potential testing strategies, including HR assays will be reviewed. Materials and methods: HR assays are performed in mice or rats in a 28-day repeat dose protocol. Animals are dosed with the test compound for 7 days, infected with the challenge infectious agent and clearance of the infectious agent evaluated during the remainder of the 28 day period. The test agent is evaluated in three separate dose levels, compared with a vehicle control and a positive immunomodulatory control. Results: The effect of a test agent is measured by comparing clearance of the infectious agent as well as functional activity of one or more individual immune functions with the vehicle control and a positive immunomodulatory control. Conclusions: TLRs may exert either therapeutic or adverse immunotoxicity and each TLR must be evaluated separately. Financial support: Burleson Research Technologies, Inc. (BRT). http://dx.doi.org/10.1016/j.toxlet.2016.07.042 S01-4 Development of novel nano-carrier with safety for vaccine against infectious diseases Y. Yoshioka Vaccine Creation Project, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan As the recent Ebola virus outbreak in Africa and worldwide influenza pandemic have powerfully demonstrated, society demands the development of vaccines for infectious diseases. However, most protein antigens, such as non-living macromolecules or protein-subunit antigens, evoke only weak or undetectable adaptive immune responses. Therefore, to develop effective vaccines, it is necessary to develop vaccine adjuvants and antigen delivery carriers. In addition, to develop optimal vaccines for clinical applications, it is important to understand the mechanism of action of vaccines on the immune system in terms of efficacy as well as safety. In this regard, we have examined the relationships between physicochemical properties, bio-distribution, intracellular localization and biological responses (safety) of nano-carrier. In addition, recently, we are trying to develop novel nano-carrier with safety for vaccine against infectious diseases. That is, our research is focused on optimizing vaccines related to drug delivery systems and safety science. In this presentation, I will introduce our recent findings about vaccine nano-carrier with efficacy and safety. http://dx.doi.org/10.1016/j.toxlet.2016.07.043 Symposia S02: Effects of pesticides on human health and ways of prevention and control S02-1 Effects of pesticides on human health and ways of prevention and control: “custody” P. Calle-Uribe NorthLAN Stewardship Manager for Crop Protection, Department of Toxicology, LAN, Bogota, Bogota, Colombia Introduction: In 2050: A third more mouths to feed. We live on a planet with more than 7 billion people all with the same or greater needs for food, clean water, sanitation, housing, mobility, education and adequate health care, that ensures their welfare.
S5
Objective: Achieve a healthy and friendly production environment through the custody of the promoter’s products of food production. Materials and methods: The company as a leader in the development of new technologies for the field company combines this factor, with the implementation of programs that allow not only the transfer of technological knowledge but also the promotion of good agricultural practices, aimed towards the same goal: better results with less inputs, all focused on the search for a sustainable agriculture is an important contribution to food security. Results: Custody of the company’s products is the best example. Strengthens the commitments of the company with the “International Code of Conduct on the Distribution and Use of Pesticides” of the Food and Agriculture Organization – FAO. This program provides information and training to representatives in the field, distributors, customers and communities in general, responsible management products companies, in order to prevent any kind of adversity that may happen in relation to the use of products, inputs, processes, operations and waste materials resulting from their work. Conclusions: The continued commitment of the company as a responsible member of society, manifested in its constant concern for continuous improvement, providing a healthy working environment and safe for all its employees and seeking primarily to minimize the environmental impact of their actions and optimize the responsible use of natural resources. Financial support: The company is in charge of project financing of custody. http://dx.doi.org/10.1016/j.toxlet.2016.07.102 S02-2 Poisonings by pesticides in Guatemala M.C. Guzmán-Quilo Department of Toxicology, Centro de Información y Asesoría Toxicológica, Escuela de Química Farmacéutica, Facultad de Ciencias Químicas y Farmacia, Universidad de San Carlos de Guatemala, Guatemala Introduction: Guatemala is a country located at the northern of Central America. One of its main economical activities is agriculture. Agriculture also represents the main percentage of employment. As the rest of Central American countries, pesticides are the main causal agent of acute poisonings. According to a research developed by PLAGSALUD-PAHO on 2000, Guatemala’s registry system had a 97.5% of under registered cases. Objective: To compare the poisoning cases registered at the national service, including the geographical occurrence, population characterization by age, with the group of pesticides according to the chemical structure and poisoning circumstances. Materials and methods: 2013–2015 registries from the Epidemiology National Center were evaluated. These were compared to the population range of ages, the chemical groups of pesticides involved and the poisoning circumstances. Results: It was determined that the number of cases registered from 2013 to 2015 has decreased. The population involved includes the two extremes of life: children and the elderly, but most of the cases are recorded to be middle age workers. The three main chemical groups of pesticides poisoning identified were: organophosphorus, paraquat and phosphine. The circumstances according to the registries go from accidental cases on working activities up to the intentional cases because of suicide attempts.