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Development of the female gametophyte in the sterile ecotype of the bolting Allium sativum L.
Scientia Horticulturae 121 (2009) 353–360
Contents lists available at ScienceDirect
Scientia Horticulturae journal homepage: www.elsevier.com/locate/scihorti
Development of the female gametophyte in the sterile ecotype of the bolting Allium sativum L. K. Winiarczyk a,*, A. Kosmala b a b
Department of Plant Anatomy and Cytology, Marie Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland Institute of Plant Genetics, Polish Academy of Sciences, Strzeszynska 34, 60-479 Poznan, Poland
A R T I C L E I N F O
A B S T R A C T
Article history: Received 9 December 2008 Received in revised form 27 January 2009 Accepted 4 February 2009
Manifestations of female sterility were investigated in a sterile bolting ecotype of Allium sativum (garlic). Morphological studies revealed interference during the formation of integuments around the nucellus and the lack of a micropylar channel and anatomical studies revealed disorders in female gametophyte formation. As the consequence of developmental abnormalities ovule abortion was observed. Finally, significant differences were revealed between 2D protein profiles of sterile ovules of A. sativum and fertile ovules of Allium tuberosum. Further possibilities of application of modern proteomic tools to identify proteins responsible for developmental changes in sterile ovules of A. sativum are discussed. Published by Elsevier B.V.
Keywords: Ovule abortion Megasporogenesis Embryo sac Protein Garlic
1. Introduction The ovule is the site of megasporogenesis, megagametogenesis and double fertilization. The mature ovule consists of tissues from both generations of the plant life cycle, the diploid sporophyte and the haploid gemetophyte. This structure is varied very deeply started from primordia initiation, specification, morphogenesis to cellular differentiation and seed formation. The intimate association of sporophytic and gametophytic tissues in ovule allows an investigation of their cellular interactions during ovule and seed development (
Contents lists available at ScienceDirect
Scientia Horticulturae journal homepage: www.elsevier.com/locate/scihorti
Development of the female gametophyte in the sterile ecotype of the bolting Allium sativum L. K. Winiarczyk a,*, A. Kosmala b a b
Department of Plant Anatomy and Cytology, Marie Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland Institute of Plant Genetics, Polish Academy of Sciences, Strzeszynska 34, 60-479 Poznan, Poland
A R T I C L E I N F O
A B S T R A C T
Article history: Received 9 December 2008 Received in revised form 27 January 2009 Accepted 4 February 2009
Manifestations of female sterility were investigated in a sterile bolting ecotype of Allium sativum (garlic). Morphological studies revealed interference during the formation of integuments around the nucellus and the lack of a micropylar channel and anatomical studies revealed disorders in female gametophyte formation. As the consequence of developmental abnormalities ovule abortion was observed. Finally, significant differences were revealed between 2D protein profiles of sterile ovules of A. sativum and fertile ovules of Allium tuberosum. Further possibilities of application of modern proteomic tools to identify proteins responsible for developmental changes in sterile ovules of A. sativum are discussed. Published by Elsevier B.V.
Keywords: Ovule abortion Megasporogenesis Embryo sac Protein Garlic
1. Introduction The ovule is the site of megasporogenesis, megagametogenesis and double fertilization. The mature ovule consists of tissues from both generations of the plant life cycle, the diploid sporophyte and the haploid gemetophyte. This structure is varied very deeply started from primordia initiation, specification, morphogenesis to cellular differentiation and seed formation. The intimate association of sporophytic and gametophytic tissues in ovule allows an investigation of their cellular interactions during ovule and seed development (